CN104628471A - Preparation method of pleurotus eryngii cultivation material - Google Patents

Abstract

The invention discloses a preparation method of a pleurotus eryngii cultivation material, which is a technology of applying lentinus edodes stems to pleurotus eryngii cultivation. Compared with the prior art, the preparation method disclosed by the invention has the following advantages: (I) the lentinus edodes stem extract is a pure natural nutrient solution containing carbohydrates, amino acids, peptides, nucleic acid and trace elements as well as biological hormone of abundant types; the lentinus edodes stem residue also contains a large quantity of un-extracted bioactive substances; a lentinus edodes stem soaking material is added and mixed with the cultivation raw material, and stacking fermentation is performed so as to promote the reproduction of beneficial microorganisms, improve the microbial flora in the materials and accelerate the catalytic decomposition of the cultivation material; (II) the fermented cultivation material contains high-activity beneficial bacteria as well as metabolites thereof and realizes a promotion effect on the growth of pleurotus eryngii hyphae, wherein the promotion effect is shown by vigorous hypha growth and strong resistance to infectious microbes; and (III) the resources such as manpower and coal are saved, environmental pollution is reduced, and thus the preparation method conforms to the concept of low carbon and environmental protection.

Description

A kind of making method of planting almond abalone mushroom material

Technical field

The making method of a kind of planting almond abalone mushroom material of the present invention, is the technology be applied to by mushroom tang in planting almond abalone mushroom, belongs to fungus growing technique field.

Background technology

Pleurotus eryngii has another name called pleurotus eryngii, is a kind of rare edible fungus variety.Its meat is plump, nutritious, long fresh-keeping period, and Pleurotus eryngii has a kind of special almond flavor, has again the crisp smooth mouth of a kind of abalone, is received by the market.

The traditional cultivating method of Pleurotus eryngii is grog cultivation, and planting material carries out inoculation culture after high pressure or normal-pressure sterilization, and the miscellaneous bacteria now in material is killed, and therefore, when planting material sends out bacterium, living contaminants is few.But along with the significantly rise of the price of labour power and fuel price, grog cultivation cost increases severely, and high-temperature sterilization also causes the loss of planting material nutritive substance, causes planting material transformation efficiency to reduce simultaneously.

Mushroom is famous and precious edible one of the medicinal fungus of holding concurrently in the world, and current China mushroom production accounts for 70% of world's mushroom ultimate production, has the equal mushroom culture in the province of more than 70% in China.Because champignon stems stiffness of foot in children, content of cellulose are higher, palatability is poor, and therefore, mushroom is being eaten raw or in the course of processing, the tang of mushroom can remove as tankage by the producer, causes the mushroom tang accounting for mushroom weight about 30% to become waste.

Summary of the invention

For the deficiencies in the prior art, the present invention adds the immersion of mushroom tang and expects to mix and stir to carry out banking up with planting material to ferment, and do not add sterilant, sterilant during windrow, the planting material after fermentation directly packs inoculation, bacterium bag living contaminants is few, and the Pleurotus eryngii quality of producing is good, output is high.

The technical solution adopted in the present invention is:

A making method for planting almond abalone mushroom material, comprises batching, fermentation, turning, pack, inoculation, sends out bacterium, concrete operation step:

(1) prepare burden: cotton seed hulls 76-80%, soya-bean cake 14-16%, mushroom tang 3-4%, lime 2.5%, calcium carbonate 1%, be all the quality of dry-matter, proportioning sum is 100%; Described mushroom tang is the tankage in the Lentnus edodes course of processing, is ground into the particle of 2mm-3mm after drying;

(2) ferment: mushroom tang particle is added clear water by 20-30 weight ratio doubly, soak 18h-24h at normal temperatures, then leach liquor is admixed in cotton seed hulls, soya-bean cake compound uniformly together with champignon stems slag, stockpile is become high 1.0m-1.2m, wide 2m-3m, the trapezoidal heap that length is not limit, the culture material often piled controls at dry weight 300kg-500kg, after carrying out heap, surrounding is patted, on heap, insert some vertical pores with the wooden stick of diameter 10cm-15cm again, at the bottom of straight through reactor, stomatal frequency is 1/m ³, finally cover straw mat by its spontaneous fermentation;

(3) turning: after material temperature reaches 60 DEG C, keeps 24h, then carries out turning, and turning every day later once, add lime during first time turning, add calcium carbonate during second time turning, altogether turning 3-4 time, time remaining 5d-6d, when stopping fermentation, the water content of planting material is 63%-65%;

(4) pack, inoculate, send out bacterium: the planting material fermented is after heat radiation, load the polypropylene plastics pocket of 17cm × 35cm × 0.004cm, stamp and plant bacterium hole, during pack, bag two ends and plant in bacterium hole and put into bacterial classification, then cultivating bag is emitted in dark bacteria room, temperature controls at 20 DEG C-22 DEG C, humid control at 60%-75%, through the cultivation of 24-26d, mycelia covers with pocket, enters management of producing mushroom routinely.

Mushroom tang is applied to the method for planting almond abalone mushroom by the present invention, and apart from outside explanation, other operation steps is all undertaken by this area routine techniques.

The present invention's advantage is compared with prior art:

1. mushroom tang leach liquor is full-natural nutritive liquid, containing glucide, amino acid, peptide class, nucleic acid and trace element, bio-hormone also containing abundant species, also the biologically active substance do not leached in a large number is had in champignon stems slag, add and expect to mix and stir to carry out banking up with culturing raw material to ferment with the immersion of mushroom tang, promote beneficial microorganism breeding, improve the microorganism species in material, accelerate the catalytic decomposition of planting material;

2. the planting material after fermentation has high reactivity probiotics and active metabolite thereof, has accelerating effect, show as prosperous, the anti-miscellaneous bacteria ability of mycelium growth vigor strong to the growth of Pleurotus eryngii mycelia;

3. save the resource such as manpower, coal, decrease environmental pollution, meet low-carbon environment-friendly theory.

Embodiment

Below in conjunction with embodiment, the invention will be further described, and what do not illustrate in detail in embodiment is all state of the art.

Embodiment 1: a kind of making method of planting almond abalone mushroom material, comprises batching, fermentation, turning, pack, inoculation, sends out bacterium, concrete operation step:

1. prepare burden: cotton seed hulls 78%, soya-bean cake 15%, mushroom tang 3.5%, lime 2.5%, calcium carbonate 1%, be all the quality of dry-matter, proportioning sum is 100%;

2. ferment: mushroom tang particle is added clear water by 20-30 weight ratio doubly, soak 18h-24h at normal temperatures, then leach liquor is admixed in cotton seed hulls, soya-bean cake compound uniformly together with champignon stems slag, stockpile is become high 1.0m-1.2m, wide 2m-3m, the trapezoidal heap that length is not limit, the culture material often piled controls at dry weight 300kg-500kg, after carrying out heap, surrounding is patted, on heap, insert some vertical pores with the wooden stick of diameter 10cm-15cm again, at the bottom of straight through reactor, stomatal frequency is 1/m ³, finally cover straw mat by its spontaneous fermentation;

3. turning: after material temperature reaches 60 DEG C, keeps 24h, then carries out turning, and turning every day later once, add lime during first time turning, add calcium carbonate during second time turning, altogether turning 3 times, time remaining 5d, when stopping fermentation, the water content of planting material is 63%-65%;

4. pack, inoculate, send out bacterium: the planting material fermented is after heat radiation, load the polypropylene plastics pocket of 17cm × 35cm × 0.004cm, stamp and plant bacterium hole, during pack, bag two ends and plant in bacterium hole and put into bacterial classification, then cultivating bag is emitted in dark bacteria room, temperature controls at 20 DEG C-22 DEG C, humid control at 60%-75%, through the cultivation of 25d, mycelia covers with pocket, enters management of producing mushroom routinely.

Embodiment 2: a kind of making method of planting almond abalone mushroom material, comprises batching, fermentation, turning, pack, inoculation, sends out bacterium, concrete operation step:

1. prepare burden: cotton seed hulls 78%, soya-bean cake 15%, mushroom tang 3.5%, lime 2.5%, calcium carbonate 1%, be all the quality of dry-matter, proportioning sum is 100%;

2. ferment: mushroom tang particle is added clear water by 20-30 weight ratio doubly, soak 18h-24h at normal temperatures, then the leach liquor after removing slag is admixed in cotton seed hulls, soya-bean cake compound uniformly, stockpile is become high 1.0m-1.2m, wide 2m-3m, the trapezoidal heap that length is not limit, the culture material often piled controls at dry weight 300kg-500kg, after carrying out heap, surrounding is patted, on heap, insert some vertical pores with the wooden stick of diameter 10cm-15cm again, at the bottom of straight through reactor, stomatal frequency is 1/m ³, finally cover straw mat by its spontaneous fermentation;

3. turning: after material temperature reaches 60 DEG C, keeps 24h, then carries out turning, and turning every day later once, add lime during first time turning, add calcium carbonate during second time turning, altogether turning 3 times, time remaining 5d, when stopping fermentation, the water content of planting material is 63%-65%;

4. pack, inoculate, send out bacterium: the planting material fermented is after heat radiation, load the polypropylene plastics pocket of 17cm × 35cm × 0.004cm, stamp and plant bacterium hole, during pack, bag two ends and plant in bacterium hole and put into bacterial classification, then cultivating bag is emitted in dark bacteria room, temperature controls at 20 DEG C-22 DEG C, humid control at 60%-75%, through the cultivation of 26d, mycelia covers with pocket, enters management of producing mushroom routinely.

Control group: Pleurotus eryngii grog is cultivated, concrete operation step:

1. prepare burden: cotton seed hulls 82%, soya-bean cake 16%, lime 1%, calcium carbonate 1%, be all the quality of dry-matter, proportioning sum is 100%;

2. spice: first cotton seed hulls, soya-bean cake, lime, calcium carbonate siccative are mixed thoroughly, then adding water stirs, makes the water content of planting material remain on 63%-65%;

3. pack, sterilizing: employing specification is the high-pressure polypropylene plastics bag charging of 17cm × 35cm × 0.005cm, carries out normal-pressure sterilization after pack, when kettle temperature reaches 100 DEG C, keeps 10h-12h;

4. cool, inoculate: naturally cool to less than 25 DEG C and access bacterial classification, then cultivating bag is emitted in dark bacteria room, temperature controls at 20 DEG C-22 DEG C, and humid control is at 60%-75%, and through the cultivation of 28d, mycelia covers with pocket, enters management of producing mushroom routinely.

Table one Pleurotus eryngii biological efficiency compares

As can be seen from Table I: the ferment effect utilizing mushroom tang leach liquor to add than single leach liquor together with the ferment effect that champignon stems slag adds is good, mycelial growth rate is fast, and bacterium bag pollution rate is low, and biological efficiency is high; Compared with control group, embodiment 1 all has obvious advantage in mycelial growth rate, anti-living contaminants and output.

Claims (1)

1. a making method for planting almond abalone mushroom material, comprises batching, fermentation, turning, pack, inoculation, sends out bacterium, it is characterized in that:

(1) prepare burden: cotton seed hulls 76-80%, soya-bean cake 14-16%, mushroom tang 3-4%, lime 2.5%, calcium carbonate 1%, be all the quality of dry-matter, proportioning sum is 100%; Described mushroom tang is the tankage in the Lentnus edodes course of processing, is ground into the particle of 2mm-3mm after drying;

(2) ferment: mushroom tang particle is added clear water by 20-30 weight ratio doubly, soak 18h-24h at normal temperatures, then leach liquor is admixed in cotton seed hulls, soya-bean cake compound uniformly together with champignon stems slag, stockpile is become high 1.0m-1.2m, wide 2m-3m, the trapezoidal heap that length is not limit, the culture material often piled controls at dry weight 300kg-500kg, after carrying out heap, surrounding is patted, on heap, insert some vertical pores with the wooden stick of diameter 10cm-15cm again, at the bottom of straight through reactor, stomatal frequency is 1/m ³, finally cover straw mat by its spontaneous fermentation;

(3) turning: after material temperature reaches 60 DEG C, keeps 24h, then carries out turning, and turning every day later once, add lime during first time turning, add calcium carbonate during second time turning, altogether turning 3-4 time, time remaining 5d-6d, when stopping fermentation, the water content of planting material is 63%-65%;

(4) pack, inoculate, send out bacterium: the planting material fermented is after heat radiation, load the polypropylene plastics pocket of 17cm × 35cm × 0.004cm, stamp and plant bacterium hole, during pack, bag two ends and plant in bacterium hole and put into bacterial classification, then cultivating bag is emitted in dark bacteria room, temperature controls at 20 DEG C-22 DEG C, humid control at 60%-75%, through the cultivation of 24-26d, mycelia covers with pocket, enters management of producing mushroom routinely.