CN108094051A - A kind of Coriaria sinica mushroom strain culture medium and preparation method thereof and the method for cultivating Coriaria sinica mushroom - Google Patents
A kind of Coriaria sinica mushroom strain culture medium and preparation method thereof and the method for cultivating Coriaria sinica mushroom Download PDFInfo
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- CN108094051A CN108094051A CN201810127057.6A CN201810127057A CN108094051A CN 108094051 A CN108094051 A CN 108094051A CN 201810127057 A CN201810127057 A CN 201810127057A CN 108094051 A CN108094051 A CN 108094051A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
Abstract
The invention discloses a kind of Coriaria sinica mushroom strain culture medium, include each component of following mass fraction：0.5~1.2 part of 75~85 parts of Coriaria sinica sawdust, 15~25 parts of wheat bran, 0.5~1.2 part of calcium superphosphate and sucrose, and Coriaria sinica sawdust is without going mouldy, water content≤12%, pulverized particles≤5mm, wheat bran is without going mouldy, water content≤13%；Calcium superphosphate 80%~95% is dissolved in water, and P2O5Mass fraction be 14%~20%.The method for also disclosing the preparation method of the culture medium simultaneously, the method for Coriaria sinica mushroom strain being obtained using the culture medium and fructification is obtained using the Coriaria sinica mushroom strain cultivation Coriaria sinica mushroom obtained.The present invention without Tube propagation, is directly separated the Coriaria sinica sawdust that access prepares, wheat bran, the medium culture of calcium superphosphate+sucrose purifies by transferring, successfully obtains Coriaria sinica mushroom Pure cultured spawn using tissue isolation；It is convenient with isolation and culture matrix manufacturing, it is separately cultured without test tube, the Spawn incubation cycle can be shortened 20 days, and Coriaria sinica mushroom fruiting body can be obtained.
Description
Technical field
The present invention relates to agricultural biological technical field, more particularly to bacterium culture medium of Coriaria sinica mushroom and preparation method thereof
And the selection method of Spawn incubation and culturing raw material.
Background technology
Coriaria sinica mushroom (Lentinus edodes Sing.), alias Coriaria sinica bacterium；Five inner perfume (or spice).Spring and autumn is born in Coriaria sinica【Coriaria sinica
(Coriaria sinica Maxinm) is subordinate to Coriariaceae, and complete stool contains coriarine】Deadwood on.The bacterium is found in Lentinus
Unique kind being grown on Coriaria sinica tree, its fragrance gene has been in long-term cultivation for other mushroom kinds at present
It loses (Shanghai Academy of Agricultural Sciences Chen Mingjie researcher), causes these mushroom kind fragrance not dense.Since Coriaria sinica mushroom is delicious clear
Perfume (or spice) has very big value of exploiting and utilizing.But the strain separating and culture technique and culturing raw material in relation to Coriaria sinica mushroom are
Blank of the prior art seriously restricts the development of the industry.
The content of the invention
The technical problem to be solved by the present invention is to overcome the not strain separating on Coriaria sinica mushroom and trainings in the prior art
The defects of technology of supporting, provides a kind of Coriaria sinica mushroom strain culture medium and preparation method thereof and the method for cultivating Coriaria sinica mushroom.
In order to solve the above technical problem, the present invention provides following technical solutions：
A kind of Coriaria sinica mushroom strain culture medium includes each component of following mass fraction：75~85 parts of Coriaria sinica sawdust, wheat bran
15~25 parts, 0.5~1.2 part of 0.5~1.2 part of calcium superphosphate and sucrose, and Coriaria sinica sawdust is without going mouldy, water content≤12%, powder
Broken particle≤5mm, wheat bran is without going mouldy, water content≤13%；Calcium superphosphate 80%~95% is dissolved in water, and the mass fraction of P2O5
For 14%~20%.Sucrose selects commercially available food-grade.
Preferably, each component of following mass fraction is included：80 parts of Coriaria sinica sawdust, 18 parts of wheat bran, 1 part of calcium superphosphate and sugarcane
1 part of sugar.
Further, the water content of culture medium is 50~55%.
The preparation method of above-mentioned Coriaria sinica mushroom strain culture medium is：Boiling water is added in into Coriaria sinica sawdust floods its whole,
Impregnate 24 it is small when, filter off moisture, add in wheat bran, calcium superphosphate, sucrose, stir evenly, adjustment water content is 50%~55%,
It bottles or packs up to Coriaria sinica mushroom strain culture medium.The method of bottling is by Coriaria sinica mushroom strain culture medium loading amount to seed bottle
Shoulder, compresses the culture medium of bottle inner surface, and tightness is：Seed bottle is inverted, culture medium does not fall；Then cleaned with clear water
Bottle is outer, then is put in gauze in bottle, cleans bottleneck shoulder, and the dry bottleneck moisture of sassafras, tampon, bottleneck is encased with brown paper beyond the Great Wall, bundle
It pricks；Then seed bottle is put into high-pressure steam sterilizing pan, in 1.5kg/cm2Under pressure, 1h is kept；The bacterium that will have been sterilized
Kind of bottle is moved on in superclean bench, allows its natural cooling, forms finished product seed bottle；The method of pack is that culture medium is packed into bacterium bag
In, the 4/5 of loading amount to bacterium bag, the culture medium of bacterium bag inner surface is compressed, tightness is：Cultivating in a fungus bag base appearance tight；So
Afterwards, bacterium bag appearance is cleaned with clear water, then the net bacterium bag top sack of sassafras in bag is put in gauze, put on collarium, bacterium is covered with collarium
Collar extension；Then bacterium bag is put into high-pressure steam sterilizing pan, in 1.5kg/cm2Under pressure, 1h is kept；The bacterium bag that will have been sterilized
It moves on in superclean bench, allows its natural cooling, form finished product bacterium bag.
Use the method for above-mentioned Coriaria sinica mushroom strain culture medium cultivation Coriaria sinica mushroom strain for
1) the fresh non-parachute-opening of same day acquisition or the Coriaria sinica mushroom fruiting body of firm parachute-opening, are taken, cuts off stem base portion, use is sterile
Absorbent cotton sassafras removes the sundries on cap, is packed into the polybag by sterilizing, is put into plastic casing and takes back laboratory in time；
2) above-mentioned polybag outer wall is wiped with 75% alcohol swab, moves on to superclean bench, under aseptic condition, drawn with scalpel
Above-mentioned one osculum of aseptic plastic bag is opened, makes just to expose at the top of cap, then cap epidermis is pruned with scalpel, is exposed in cap
White tissues access seed bottle culture with stem engaging portion covering with scalpel picking white tissues 3~5mm size tissue blocks
In the middle part of base；
3) seed bottle, is placed in 20 DEG C~25 DEG C insulating box cultures, the sprouting shape of the tissue block of observation access daily
Condition：It is normal, strain separating success if the tissue block and pollution-free, the single bacterium colony of surrounding；Otherwise it is pollution, detects in time；
It will normally sprout, and form the seed bottle of single bacterium colony, when it is 2~4cm bacterium colonies that a loop diameter is formed on culture medium, in time
Switching, is allowed to purify, and switching forms Coriaria sinica mushroom strain afterwards more than twice.
The identification of Coriaria sinica mushroom strain
Colony characteristics：White, mycelia villiform, sprawl growth, colony edge is neat, edge and central part color one
It causes, it is homogeneous.
Mycelia body characteristics：White mycelium is visually observed, mycelia is transparent under microscope, branch, has every primary mycelium thousand
Carefully, secondary mycelium is sturdy.
Further, the method for strain transfer is under superclean bench aseptic condition, with transfer needle picking colony edge
Mycelium is transferred on another seed bottle culture medium, is placed in 20 DEG C~25 DEG C insulating box cultures, the thalline life of observation access daily
Long situation, mycelia material feeding, growth is neat, dense, healthy and strong, pollution-free.
Using the method for above-mentioned cultivating bacterial spawn Coriaria sinica mushroom, include the following steps：
1) bacterium bag outer wall is wiped with 75% alcohol swab, moves on to superclean bench, under aseptic condition, take 1.5~2cm sizes
In the middle part of Coriaria sinica mushroom strain block access cultivating in a fungus bag base, it is made to have with culture medium and is preferably contacted；
2) 20 DEG C~25 DEG C insulating box cultures, are placed in, the sprouting state of the strain block of observation access daily：It is if described
Strain block and pollution-free, the single bacterium colony of surrounding, are normal, bacterium bag is transferred successfully；
2) above-mentioned bacterium bag is normally sprouted, forms the bacterium bag of single bacterium colony, wait after covering with bacterium bag, its annesl is allowed to go out
Mushroom.
Following methods can also be used：
1), take more than diameter 4cm, felling obtains Coriaria sinica branch after 15 days, is cut into 0.8~1.2m, forms finished product bacterium material；
2), punched on bacterium material, access cultured Coriaria sinica mushroom strain, it is made to have with Coriaria sinica bacterium material and is preferably contacted；From
Right temperature conditionss are after mycelia covers with bacterium material, annesl fruiting.
Superclean bench in the present invention refers to adapt to modernization industry, opto-electronics, bio-pharmaceuticals and scientific research
The demand of local working areas cleanliness factor is designed in the fields such as experiment.Superclean bench principle be in specific space,
Room air is considered through prefilter chuck, and plenum chamber is pressed by Small Centrifugal Fan, then through high-efficiency air filter two level mistake
Filter, the clean gas flow blown out from high-efficiency air filter outlet air surface have certain and uniform sectional wind velocity, can exclude work
Make the original air in area, dust granules and biologic grain are taken away, to form the working environment of sterile high-cleanness.Ultra-clean work
The advantages of platform is easy to operate freely, and pleasant, work efficiency, readiness time is short, and start can be operated for 10 minutes or more, base
It can be used at any time on this.In factorial praluction, inoculation workload is very big, and when needing often muchly to work, superclean bench is
Highly desirable equipment.Superclean bench makees blowing power, power 145-260W or so by three phase electric machine, and air is passed through by special
" super filter " after-blow of microcellular foam lamella overlapping group send out, form the ultra-clean of continuously dust-free sterile
Lamina air flow, i.e., so-called " effective special air ", it eliminates the dust more than 0.3 μm, fungi and bacterial spore etc..It is super
The flow velocity of net air is 24-30m/min, this has prevented neighbouring air from being polluted caused by may harassing enough, such flow velocity
It will not interfere and the calcination of instrument etc. is sterilized using alcolhol burner or alcolhol burner.Staff just grasps under such aseptic condition
Make, keep sterilizable material not contaminated in seeded process is shifted.But just in case operation midway runs into power failure, exposed to not filtering
Material in air is just difficult to pollution of escaping by luck.At this moment answer rapid power cut-off, and mark made on bottle, it is interior in material as locate
Multiplicative stage is then no longer serve as being proliferated and being transferred to culture of rootage later.It is such as general production material, it is extremely abundant to abandon
It goes.It plants and uses if place took root, then after can remaining.
The advantageous effect that is reached of the present invention is：
1st, the present invention, without Tube propagation, is directly separated the Coriaria sinica sawdust that access prepares, bran using tissue isolation
Skin, the medium culture of calcium superphosphate+sucrose are purified by transferring, successfully obtain Coriaria sinica mushroom Pure cultured spawn；
2nd, the present invention has isolation and culture matrix manufacturing convenient, is separately cultured without test tube, shortens the Spawn incubation cycle.
Specific embodiment
The preferred embodiment of the present invention is illustrated below, it should be understood that preferred embodiment described herein is only used
In the description and interpretation present invention, it is not intended to limit the present invention.
Embodiment 1
A kind of Coriaria sinica mushroom strain culture medium includes each component of following mass fraction：75 parts of Coriaria sinica sawdust, wheat bran 15
Part, 0.5 part of 0.5 part of calcium superphosphate and sucrose, and Coriaria sinica sawdust is without going mouldy, water content≤12%, pulverized particles≤5mm, wheat bran
Nothing is gone mouldy, water content≤13%；Calcium superphosphate 80%~95% is dissolved in water, and the mass fraction of P2O5 is 14%~20%.Sugarcane
Sugar selects commercially available food-grade；The water content of culture medium is 50~55%.
Preparation method is：Boiling water is added in into Coriaria sinica sawdust floods its whole, when immersion 24 is small, filters off moisture, adds
Enter wheat bran, calcium superphosphate, sucrose, stir evenly, adjustment water content is 50%~55%, bottles or packs up to Coriaria sinica Lenlinus edodes
Kind culture medium.The method of bottling is that Coriaria sinica mushroom strain culture medium loading amount to seed bottle shoulder is compressed to the culture of bottle inner surface
Base, tightness are：Seed bottle is inverted, culture medium does not fall；Then use outside clear water detergent bottle, then put in gauze in bottle,
Bottleneck shoulder is cleaned, the dry bottleneck moisture of sassafras, tampon, encases bottleneck with brown paper, tie up beyond the Great Wall；Then seed bottle is put into high pressure
In steam sterilization pan, in 1.5kg/cm2Under pressure, 1h is kept；The seed bottle to have sterilized is moved on in superclean bench, is allowed
Its natural cooling forms finished product seed bottle；Culture medium is is fitted into bacterium bag by the method for pack, and the 4/5 of loading amount to bacterium bag, it compresses
The culture medium of bacterium bag inner surface, tightness are：Cultivating in a fungus bag base appearance tight；Then, bacterium bag appearance is cleaned with clear water, then
The net bacterium bag top sack of sassafras in bag is put in gauze, collarium is put on, collarium mouth is covered with collarium lid；Then bacterium bag is put into high pressure steaming
In vapour autoclave, in 1.5kg/cm2Under pressure, 1h is kept；The bacterium bag to have sterilized is moved on in superclean bench, allows it certainly
So cooling forms finished product bacterium bag.
Use the method for above-mentioned Coriaria sinica mushroom strain culture medium cultivation Coriaria sinica mushroom strain for
1), the Coriaria sinica mushroom fruiting body of the fresh non-parachute-opening on the day of Guizhou Province Dafang County gathers or firm parachute-opening, cuts off bacterium
Handle base portion, removes the sundries on cap with sterile absorbent cotton sassafras, is packed into the polybag by sterilizing, is put into plastic casing and timely band
Go back to laboratory；For example the Coriaria sinica mushroom fruiting body of Guizhou Province Dafang County can be selected；
2) above-mentioned polybag outer wall is wiped with 75% alcohol swab, moves on to superclean bench, under aseptic condition, drawn with scalpel
Above-mentioned one osculum of aseptic plastic bag is opened, makes just to expose at the top of cap, then cap epidermis is pruned with scalpel, is exposed in cap
White tissues access seed bottle culture with stem engaging portion covering with scalpel picking white tissues 3~5mm size tissue blocks
In the middle part of base；
3) seed bottle, is placed in 20 DEG C~25 DEG C insulating box cultures, the sprouting shape of the tissue block of observation access daily
Condition：It is normal, strain separating success if the tissue block and pollution-free, the single bacterium colony of surrounding；Otherwise it is pollution, detects in time；
It will normally sprout, and form the seed bottle of single bacterium colony, when it is 2~4cm bacterium colonies that a loop diameter is formed on culture medium, in time
Switching, is allowed to purify, and switching forms Coriaria sinica mushroom strain afterwards more than twice.
The method of strain transfer is under superclean bench aseptic condition, with transfer needle picking colony edge mycelium, is turned
It is connected on another seed bottle culture medium, is placed in 20 DEG C~25 DEG C insulating box cultures, the thalli growth situation of observation access daily, bacterium
Silk material feeding, growth is neat, dense, healthy and strong, pollution-free.
Using the method for above-mentioned cultivating bacterial spawn Coriaria sinica mushroom, include the following steps：
1) bacterium bag outer wall is wiped with 75% alcohol swab, moves on to superclean bench, under aseptic condition, take 1.5~2cm sizes
In the middle part of Coriaria sinica mushroom strain block access cultivating in a fungus bag base, it is made to have with culture medium and is preferably contacted；
2) 20 DEG C~25 DEG C insulating box cultures, are placed in, the sprouting state of the strain block of observation access daily：It is if described
Strain block and pollution-free, the single bacterium colony of surrounding, are normal, bacterium bag is transferred successfully；
2) above-mentioned bacterium bag is normally sprouted, forms the bacterium bag of single bacterium colony, wait after covering with bacterium bag, its annesl is allowed to go out
Mushroom.
Embodiment 2
A kind of Coriaria sinica mushroom strain culture medium includes each component of following mass fraction：75~85 parts of Coriaria sinica sawdust, wheat bran
15~25 parts, 0.5~1.2 part of 0.5~1.2 part of calcium superphosphate and sucrose, and Coriaria sinica sawdust is without going mouldy, water content≤12%, powder
Broken particle≤5mm, wheat bran is without going mouldy, water content≤13%；Calcium superphosphate 80%~95% is dissolved in water, and the mass fraction of P2O5
For 14%~20%.Sucrose selects commercially available food-grade.
The preparation method of the present embodiment bacterium culture medium and the cultural method of strain are the same as embodiment 1.
Using the method for above-mentioned cultivating bacterial spawn Coriaria sinica mushroom, include the following steps：
1), take more than diameter 4cm, felling obtains Coriaria sinica branch after 15 days, is cut into 0.8~1.2m, forms finished product bacterium material；
2), punched on bacterium material, access cultured Coriaria sinica mushroom strain, it is made to have with Coriaria sinica bacterium material and is preferably contacted；From
Right temperature conditionss are after mycelia covers with bacterium material, annesl fruiting.
Embodiment 3
A kind of Coriaria sinica mushroom strain culture medium includes each component of following mass fraction：80 parts of Coriaria sinica sawdust, wheat bran 18
Part, 1 part of 1 part of calcium superphosphate and sucrose, and Coriaria sinica sawdust, without going mouldy, water content≤12%, pulverized particles≤5mm, wheat bran is without mould
Become, water content≤13%；Calcium superphosphate 80%~95% is dissolved in water, and the mass fraction of P2O5 is 14%~20%.Sucrose selects
With commercially available food-grade.
The preparation method of the present embodiment bacterium culture medium and the cultural method of strain are the same as embodiment 1.
The method of this implementation cultivation Coriaria sinica mushroom is the same as embodiment 1.
1-3 of the embodiment of the present invention can obtain the entity of Coriaria sinica mushroom strain.The present invention uses tissue isolation, without
Tube propagation is crossed, is directly separated the Coriaria sinica sawdust that access prepares, wheat bran, the medium culture of calcium superphosphate+sucrose, by turning
Purifying is connect, successfully obtains Coriaria sinica mushroom Pure cultured spawn.The present invention has isolation and culture matrix manufacturing convenient, is separated without test tube
Culture, shortens the Spawn incubation cycle --- and it only needs 60 days, and generally goes through test tube separation in the prior art, purify, culture obtains
Parent species, then original seed of transferring, cultigen, it is necessary to 80-90 days, this technology 60-70 days can shorten the Spawn incubation cycle 20 days.It is existing
There are other seeds sawdusts of generally use in technology or use other seeds arboriculture Coriaria sinica mushrooms, nutrient growth can only be carried out
(i.e. mycelial growth), it is impossible to carry out robustness growth (i.e. sporophore growth), this technology is considered to be worth doing using Coriaria sinica trees or Coriaria sinica tree
Cultivation basswood Coriaria sinica bacterium, can obtain Coriaria sinica mushroom fruiting body.
Finally it should be noted that：The foregoing is only a preferred embodiment of the present invention, is not intended to limit the invention,
Although the present invention is described in detail with reference to the foregoing embodiments, for those skilled in the art, still may be used
To modify to the technical solution recorded in foregoing embodiments or carry out equivalent substitution to which part technical characteristic.
Within the spirit and principles of the invention, any modifications, equivalent replacements and improvements are made should be included in the present invention's
Within protection domain.
Claims (10)
1. a kind of Coriaria sinica mushroom strain culture medium, which is characterized in that include each component of following mass fraction：Coriaria sinica sawdust 75~
85 parts, 15~25 parts of wheat bran, 0.5~1.2 part of 0.5~1.2 part of calcium superphosphate and sucrose, and Coriaria sinica sawdust is without going mouldy, water content
≤ 12%, pulverized particles≤5mm, wheat bran is without going mouldy, water content≤13%；Calcium superphosphate 80%~95% is dissolved in water, and P2O5's
Mass fraction is 14%~20%.
2. Coriaria sinica mushroom strain culture medium as described in claim 1, which is characterized in that include each group of following mass fraction
Point：1 part of 80 parts of Coriaria sinica sawdust, 18 parts of wheat bran, 1 part of calcium superphosphate and sucrose.
3. Coriaria sinica mushroom strain culture medium as claimed in claim 1 or 2, which is characterized in that the water content of culture medium for 50~
55%.
4. a kind of preparation method of Coriaria sinica mushroom strain culture medium as described in claim 1, which is characterized in that Coriaria sinica sawdust
Middle addition boiling water floods its whole, when immersion 24 is small, filters off moisture, adds in wheat bran, calcium superphosphate, sucrose, stir evenly,
It is 50%~55% to adjust water content, bottles or packs up to Coriaria sinica mushroom strain culture medium.
5. the preparation method of Coriaria sinica mushroom strain culture medium as claimed in claim 4, which is characterized in that the method for bottling is will
Coriaria sinica mushroom strain culture medium loading amount compresses the culture medium of bottle inner surface to seed bottle shoulder, and tightness is：Seed bottle is fallen
It puts, culture medium does not fall；Then use outside clear water detergent bottle, then put in gauze in bottle, clean bottleneck shoulder, the dry bottleneck water of sassafras
Point, tampon, encases bottleneck with brown paper, ties up beyond the Great Wall；Then seed bottle is put into high-pressure steam sterilizing pan, in 1.5kg/
cm2Under pressure, 1h is kept；The seed bottle to have sterilized is moved on in superclean bench, allows its natural cooling, forms finished product bacterium
Kind bottle.
6. the preparation method of Coriaria sinica mushroom strain culture medium as claimed in claim 4, which is characterized in that the method for pack is will
Culture medium is fitted into bacterium bag, and the 4/5 of loading amount to bacterium bag, the culture medium of bacterium bag inner surface is compressed, tightness is：Cultivating in a fungus bag base
Appearance tight；Then, bacterium bag appearance is cleaned with clear water, then the net bacterium bag top sack of sassafras in bag is put in gauze, put on collarium,
Collarium mouth is covered with collarium lid；Then bacterium bag is put into high-pressure steam sterilizing pan, in 1.5kg/cm2Under pressure, 1h is kept；It will
The bacterium bag to have sterilized is moved on in superclean bench, allows its natural cooling, forms finished product bacterium bag.
7. a kind of side of Coriaria sinica mushroom strain culture medium cultivation Coriaria sinica mushroom strain prepared by method using described in claim 4
Method, which is characterized in that
1) the fresh non-parachute-opening of same day acquisition or the Coriaria sinica mushroom fruiting body of firm parachute-opening, are taken, stem base portion is cut off, uses sterile absorbent
Cotton sassafras removes the sundries on cap, is packed into the polybag by sterilizing, is put into plastic casing and takes back laboratory in time；
2) above-mentioned polybag outer wall is wiped with 75% alcohol swab, moves on to superclean bench, under aseptic condition, scratched with scalpel
One osculum of aseptic plastic bag is stated, makes just to expose at the top of cap, then cap epidermis is pruned with scalpel, exposes the white in cap
Tissue, is accessed with scalpel picking white tissues 3~5mm size tissue blocks in seed bottle culture medium covering with stem engaging portion
Portion；
3) seed bottle, is placed in 20 DEG C~25 DEG C insulating box cultures, the sprouting state of the tissue block of observation access daily：If
The tissue block and pollution-free, the single bacterium colony of surrounding are normal, strain separating success；Otherwise it is pollution, detects in time；It will just
It often sprouts, forms the seed bottle of single bacterium colony, when it is 2~4cm bacterium colonies that a loop diameter is formed on culture medium, turn in time
It connects, is allowed to purify, switching forms Coriaria sinica mushroom strain afterwards more than twice.
8. the method for cultivation Coriaria sinica mushroom strain as claimed in claim 7, which is characterized in that the method for strain transfer is super
Under net workbench aseptic condition, with transfer needle picking colony edge mycelium, it is transferred on another seed bottle culture medium, is placed in
20 DEG C~25 DEG C insulating box cultures, the thalli growth situation of observation access daily, mycelia material feeding, growth is neat, dense, healthy and strong, nothing
Pollution.
9. a kind of method of Coriaria sinica mushroom strain cultivation Coriaria sinica mushroom using described in claim 7, which is characterized in that including such as
Lower step：
1) bacterium bag outer wall is wiped with 75% alcohol swab, moves on to superclean bench, under aseptic condition, take the Coriaria sinica of 1.5~2cm sizes
In the middle part of mushroom strain block access cultivating in a fungus bag base, it is made to have with culture medium and is preferably contacted；
2) 20 DEG C~25 DEG C insulating box cultures, are placed in, the sprouting state of the strain block of observation access daily：If the strain
Block and pollution-free, the single bacterium colony of surrounding, are normal, bacterium bag is transferred successfully；
2) above-mentioned bacterium bag is normally sprouted, forms the bacterium bag of single bacterium colony, wait after covering with bacterium bag, allow its annesl fruiting.
A kind of 10. method using Coriaria sinica mushroom strain cultivation Coriaria sinica mushroom described in claim 7, which is characterized in that including such as
Lower step：
1), take more than diameter 4cm, felling obtains Coriaria sinica branch after 15 days, is cut into 0.8~1.2m, forms finished product bacterium material；
2), punched on bacterium material, access cultured Coriaria sinica mushroom strain, it is made to have with Coriaria sinica bacterium material and is preferably contacted；Natural epidemic disaster in booth
Degree condition is after mycelia covers with bacterium material, annesl fruiting.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110278829A (en) * | 2019-08-01 | 2019-09-27 | 遵义市康绿诚农业科技有限责任公司 | A method of cultivation Coriaria sinica mushroom |
| CN113207545A (en) * | 2021-04-21 | 2021-08-06 | 湄潭县众志菌业有限公司 | Wild-imitating shiitake mushroom cultivation method |
| CN114711092A (en) * | 2022-05-26 | 2022-07-08 | 遵义市农业科学研究院 | Cultivation method of wild-like coriaria |
| CN114731905A (en) * | 2022-05-23 | 2022-07-12 | 贵州省马桑菌生物科技有限公司 | Plastic biological particle strain preparation formula |
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| CN103980027A (en) * | 2014-05-29 | 2014-08-13 | 贵州省山地资源研究所 | Volvariella brumalis strain culture medium and preparation method thereof, and strain culture method by using culture medium |
| CN104557211A (en) * | 2015-01-20 | 2015-04-29 | 浙江大学 | Special strain culture medium for mushroom liquefaction and corresponding culture method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110278829A (en) * | 2019-08-01 | 2019-09-27 | 遵义市康绿诚农业科技有限责任公司 | A method of cultivation Coriaria sinica mushroom |
| CN113207545A (en) * | 2021-04-21 | 2021-08-06 | 湄潭县众志菌业有限公司 | Wild-imitating shiitake mushroom cultivation method |
| CN114731905A (en) * | 2022-05-23 | 2022-07-12 | 贵州省马桑菌生物科技有限公司 | Plastic biological particle strain preparation formula |
| CN114711092A (en) * | 2022-05-26 | 2022-07-08 | 遵义市农业科学研究院 | Cultivation method of wild-like coriaria |
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