JP6085889B2 - Straw cultivation method using reusable fiber as a base material, and cultivation medium used therefor - Google Patents
Straw cultivation method using reusable fiber as a base material, and cultivation medium used therefor Download PDFInfo
- Publication number
- JP6085889B2 JP6085889B2 JP2014524685A JP2014524685A JP6085889B2 JP 6085889 B2 JP6085889 B2 JP 6085889B2 JP 2014524685 A JP2014524685 A JP 2014524685A JP 2014524685 A JP2014524685 A JP 2014524685A JP 6085889 B2 JP6085889 B2 JP 6085889B2
- Authority
- JP
- Japan
- Prior art keywords
- cultivation
- culture
- culture medium
- mushrooms
- base material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
- A01G18/64—Cultivation containers; Lids therefor
- A01G18/66—Cultivation bags
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
- A01G18/64—Cultivation containers; Lids therefor
- A01G18/68—Cultivation bottles
Description
本発明は、菌床栽培により茸を栽培する方法に関する。より詳細には、培養基材を再利用することができ、廃棄物の軽減に寄与できる茸の栽培方法に関する。更に、本発明は当該栽培方法に使用される栽培用培地に関する。 The present invention relates to a method for cultivating straw by fungus bed cultivation. More specifically, the present invention relates to a method for growing straw that can reuse the culture substrate and contribute to the reduction of waste. Furthermore, this invention relates to the culture medium used for the said cultivation method.
近年、健康志向の高まりを受け、健康食品として茸が注目されている。従来、多くの市販の茸は、おが屑に水と栄養を加えた栽培用培地を用いて人工菌床栽培により生産されている。しかしながら、茸の菌床栽培の普及と茸の生産量の増加により、栽培後に廃棄物となるおが屑の量が飛躍的に増加している。そのため、使用後のおが屑の使い捨てによる廃棄物の処理方法が悩みとなると共に、おがくずの将来的な供給不足が危惧されている。 In recent years, salmon has attracted attention as a health food in response to growing health consciousness. Conventionally, many commercially available cocoons have been produced by artificial fungus bed cultivation using a cultivation medium in which water and nutrients are added to sawdust. However, the amount of sawdust, which becomes waste after cultivation, has increased dramatically due to the spread of fungus bed cultivation and the increase in production of straw. For this reason, there are concerns about the waste disposal method by disposable sawdust after use, and there is a concern about the future supply shortage of sawdust.
そこで、従来、茸の栽培用培地において、おが屑の使用量を低減させることが試みられている。例えば、特許文献１には、廃棄紙材を小片状に破砕して得た破砕紙材とおが屑を混ぜ合わせた栽培用培地を用いて、茸を菌床栽培する方法が提案されている。しかしながら、特許文献１の方法では、あくまで、おが屑の減量が可能になるに止まり、おが屑を使用せずに茸の菌床栽培を可能にしている技術ではない。更に、特許文献１の方法では、依然として、栽培後の栽培用培地の再利用が困難であり、資源の有効活用に資するとは言い難い。 Therefore, conventionally, attempts have been made to reduce the amount of sawdust used in the cultivation medium for straw. For example, Patent Document 1 proposes a method for cultivating straw by using a cultivation medium in which waste paper material is obtained by crushing waste paper material into small pieces and mixing sawdust. However, the method of Patent Document 1 is not limited to the technology that enables the reduction of sawdust, and enables the cultivation of potato beds without using sawdust. Furthermore, in the method of Patent Document 1, it is still difficult to reuse the culture medium after cultivation, and it is difficult to say that it contributes to effective utilization of resources.
また、おが屑を使用せずに茸の菌床栽培を可能にしている技術についても、幾つか報告されている。例えば、特許文献２には、ガラスビーズ、セラミックボール等の球状成型物を培養基材として使用することにより、栽培後でも培養基材を回収して再利用できることが開示されている。更に、特許文献３には、茸の栽培用培地において、合成樹脂材からなる粒状の培養基材を使用することにより、培養基材の再利用が可能であることが開示されている。しかしながら、特許文献２及び３の方法では、培養基材以外に、バーミキュライト等の保水材や、米糠、ふすま等の保水性の不溶性栄養源を比較的多量に使用することが必要とされているため、栽培後の培地から培養基材を分離するのが困難であり、培養基材を洗浄する装置を開発しなければならないという問題点がある。更に、保水材や保水性の不溶性栄養源は、栽培後には廃棄物となるため、廃棄物の低減という点でも欠点がある。 In addition, some techniques have been reported that make it possible to cultivate potato beds without using sawdust. For example, Patent Document 2 discloses that by using a spherical molded product such as glass beads and ceramic balls as a culture substrate, the culture substrate can be recovered and reused even after cultivation. Furthermore, Patent Document 3 discloses that the culture substrate can be reused by using a granular culture substrate made of a synthetic resin material in the cultivation medium for strawberries. However, in the methods of Patent Documents 2 and 3, in addition to the culture substrate, it is necessary to use a relatively large amount of a water retention material such as vermiculite and a water retention insoluble nutrient source such as rice bran and bran. However, it is difficult to separate the culture substrate from the cultured medium, and there is a problem that an apparatus for cleaning the culture substrate must be developed. Furthermore, the water-retaining material and the water-retaining insoluble nutrient source become waste after cultivation, and thus have a drawback in terms of reducing waste.
本発明の目的は、栽培後に培養基材を簡便に回収して再利用でき、栽培後に生じる廃棄物を大幅に低減できる茸の栽培技術を提供することである。 An object of the present invention is to provide a cultivation technique for straw that can easily collect and reuse a culture substrate after cultivation, and can greatly reduce waste generated after cultivation.
本発明は、前記課題を解決すべく鋭意検討を行ったところ、再利用可能な繊維基材を培養基材として用いて、これに水と栄養源を含浸させた栽培用培地を使用すると、茸の栽培が可能になることを見出した。また、当該栽培用培地は、栽培後に培養基材を容易に回収でき、既存の洗浄装置を使用して簡便に洗浄して培養基材として再利用が可能になり、茸の栽培によって生じる廃棄物を大幅に低減できることも見出した。本発明は、これらの知見に基づいて、更に検討を重ねることにより完成したものである。 The present invention has been intensively studied to solve the above problems. As a result, when a reusable fiber base material is used as a culture base material, and a culture medium impregnated with water and nutrient sources is used, It became possible to grow. In addition, the culture medium can easily recover the culture base material after cultivation, can be easily washed using an existing cleaning device, and can be reused as a culture base material. It has also been found that can be greatly reduced. The present invention has been completed by further studies based on these findings.
即ち、本発明は、下記に掲げる態様の茸の栽培方法、及び茸の栽培用培地を提供する。
項１． 再利用可能な繊維基材、水、及び栄養源を含む栽培用培地を用いて、茸を菌床栽培することを特徴とする、茸の栽培方法。
項２． 再利用可能な繊維基材が、線状繊維基材又はシート状繊維基材である、項１に記載の茸の栽培方法。
項３． 茸の菌床栽培が、ビン栽培又は袋栽培にて行われる、項１又は２に記載の茸の栽培方法。
項４． 茸の栽培後に栽培用培地から繊維基材を回収し、当該繊維基材を培養基材として再利用して茸の栽培を繰り返し行う、項１〜３のいずれかに記載の茸の栽培方法。
項５． 茸が、ヒラタケ、エノキタケ、エリンギ、タモギタケ、ブナシメジ、シイタケ及びマイタケよりなる群から選択される少なくとも１種である、項１〜４のいずれかに記載の茸の栽培方法。
項６． 茸が、エノキタケ、ヒラタケ、エリンギ、タモギタケ、及びブナシメジよりなる群から選択される少なくとも１種であり、菌床栽培がビン栽培にて行われる、項１〜５のいずれかに記載の茸の栽培方法。
項７． 茸が、シイタケ、タモギタケ、ヒラタケ、及びマイタケよりなる群から選択される少なくとも１種であり、菌床栽培が袋栽培にて行われる、項１〜５のいずれかに記載の茸の栽培方法。
項８． 再利用可能な繊維基材、水、及び栄養源を含むことを特徴とする、茸の栽培用培地。
項９． 再利用可能な繊維基材が、線状繊維基材又はシート状繊維基材である、項８に記載の茸の栽培用培地。
項１０． ビン栽培用の培地である、項８又は９に記載の茸の栽培用培地。
項１１． 袋栽培用の培地である、項８又は９に記載の茸の栽培用培地。
項１２． ヒラタケ、エノキタケ、エリンギ、タモギタケ、ブナシメジ、シイタケ及びマイタケよりなる群から選択される少なくとも１種の茸の栽培に使用される、項８〜１１のいずれかに記載の茸の栽培用培地。
項１３． エノキタケ、ヒラタケ、エリンギ、タモギタケ、及びブナシメジよりなる群から選択される少なくとも１種の茸の栽培に使用される、項１０に記載の茸の栽培用培地。
項１４． シイタケ、タモギタケ、ヒラタケ、及びマイタケよりなる群から選択される少なくとも１種の茸の栽培に使用される、項１１に記載の茸の栽培用培地。That is, this invention provides the cultivation method of the grape of the aspect hung up below, and the culture medium for cultivation of straw.
Item 1. A method for cultivating persimmons, comprising cultivating persimmon beds using a reusable fiber base material, water, and a culture medium containing a nutrient source.
Item 2. Item 2. The cultivation method for strawberries according to Item 1, wherein the reusable fiber substrate is a linear fiber substrate or a sheet fiber substrate.
Item 3. Item 3. A method for cultivating a koji according to item 1 or 2, wherein the fungus bed cultivation of koji is performed by bottle cultivation or bag cultivation.
Item 4. Item 4. The method for cultivating cocoons according to any one of Items 1 to 3, wherein a fiber base material is recovered from the culture medium after cultivating the cocoon, and the fiber base material is reused as a culture substrate to repeatedly cultivate the cocoon.
Item 5. Item 5. The method for cultivating a koji according to any one of items 1 to 4, wherein the koji is at least one selected from the group consisting of oyster mushrooms, enokitake mushrooms, eringgi, tamogitake mushrooms, beech shimeji mushrooms, shiitake mushrooms and maitake mushrooms.
Item 6. Item 6. The cultivation of koji according to any one of items 1 to 5, wherein the koji is at least one selected from the group consisting of enokitake mushrooms, oyster mushrooms, eringi, tamogitake, and bunashimeji, and fungus bed cultivation is performed in bottle cultivation. Method.
Item 7. Item 6. The method for cultivating a strawberry according to any one of Items 1 to 5, wherein the cocoon is at least one selected from the group consisting of shiitake mushroom, bamboo shoot, oyster mushroom, and maitake.
Item 8. A culture medium for straw cultivation, comprising a reusable fiber substrate, water, and a nutrient source.
Item 9. Item 10. The cultivation medium for strawberries according to Item 8, wherein the reusable fiber substrate is a linear fiber substrate or a sheet fiber substrate.
Item 10. Item 10. The cultivation medium for strawberries according to Item 8 or 9, which is a medium for bottle cultivation.
Item 11. Item 10. A medium for cultivation of strawberries according to Item 8 or 9, which is a medium for bag cultivation.
Item 12. Item 12. The medium for cultivation of koji according to any one of items 8 to 11, which is used for cultivation of at least one kind of koji selected from the group consisting of oyster mushrooms, enokitake mushrooms, eringi, tamagotake, beech shimeji mushrooms, shiitake mushrooms and maitake mushrooms.
Item 13. Item 11. The medium for cultivation of koji according to item 10, which is used for cultivation of at least one kind of koji selected from the group consisting of enokitake, oyster mushrooms, eringi, tamogitake, and bunashimeji.
Item 14. Item 12. The cultivation medium for strawberries according to Item 11, which is used for cultivation of at least one kind of strawberries selected from the group consisting of shiitake mushrooms, bamboo shoots, oyster mushrooms, and maitakes.
本発明によれば、おが屑を使用せずとも、茸の菌床栽培が可能になるので、おが屑の使い捨てによる廃棄物問題と将来的なおが屑供給問題の悩みを解消することができる。また、本発明によれば、栽培後の栽培用培地から、培養基材を簡便に回収し、洗濯機等の既存の洗浄装置で容易に洗浄して、培養基材として再利用できるので、廃棄物を大幅に低減することができる。更に、本発明において、培養基材として使用される培養基材は、それ自体保水性を備えるので、保水材を使用せずに茸の菌床栽培が可能になり、更には、将来的に茸の水耕栽培を実現する上での基幹技術にもなり得る。 According to the present invention, the fungus bed can be cultivated without using sawdust, so the problems of waste due to disposable sawdust and future sawdust supply problems can be solved. In addition, according to the present invention, the culture substrate can be easily recovered from the cultivation medium after cultivation, easily washed with an existing washing apparatus such as a washing machine, and reused as the culture substrate. Things can be greatly reduced. Furthermore, in the present invention, the culture substrate used as the culture substrate itself has water retention, so that the fungus bed can be cultivated without using a water retention material. It can also be a basic technology for realizing hydroponics.
また、従来の茸の菌床栽培では、栽培する茸の種類に応じておが屑の種類を選ぶ必要があり、栽培用培地は茸の種類により、おが屑の種類を変えなければならなかった。しかし、本発明によれば、おが屑を使用せずとも栽培用培地を調製できるので、栽培用培地の調製を簡便に行えるという利点もある。 Further, in the conventional fungus bed cultivation of straw, it is necessary to select the kind of sawdust according to the kind of straw to be cultivated, and the cultivation medium has to change the kind of sawdust depending on the kind of straw. However, according to the present invention, since the cultivation medium can be prepared without using sawdust, there is also an advantage that the cultivation medium can be easily prepared.
更に、従来の菌床栽培では培地を充填した栽培瓶が重くなり、瓶の運搬などで多大の労力を必要とした。これに対して、本発明では、おが屑培地に比べて重さを25％程度にまで軽減させることができ、更に培養液の工夫次第では栽培瓶の大きさ自体も小さくすることができる可能性がある。また、おが屑培地を用いる場合はその原料であるおが屑を貯蔵する施設が必要となるが、本発明ではおが屑を使用しないため、貯蔵場所を無くしてしまうことができる。そして、通常のおが屑培地では殺菌を行うために長時間の高温、高圧滅菌を要するが、本発明では滅菌時間を大幅に短縮することもできる。このように、本発明には、菌床栽培の技術の簡便化と現場の合理化を図ることができ、従来の菌床栽培に比して商業上の大きな利点がある。 Furthermore, in the conventional fungus bed cultivation, the cultivation bottle filled with the culture medium becomes heavy, and much labor is required for carrying the bottle. In contrast, in the present invention, the weight can be reduced to about 25% compared to sawdust medium, and the size of the cultivation bottle itself may be reduced depending on the culture solution. is there. Moreover, when using a sawdust culture medium, the facility which stores the sawdust which is the raw material is needed, but since a sawdust is not used in this invention, a storage place can be eliminated. And, in normal sawdust medium, long time high temperature and high pressure sterilization is required for sterilization, but in the present invention, the sterilization time can be greatly shortened. As described above, the present invention can simplify the technique of fungus bed cultivation and rationalize the field, and has a great commercial advantage compared to conventional fungus bed cultivation.
本発明の茸の栽培方法は、繊維基材、水、及び栄養源を含む栽培用培地を用いて、茸を菌床栽培することを特徴とする。以下、本発明について、詳述する。 The method for cultivating cocoons of the present invention is characterized in that the cocoons are cultivated on a fungus using a culture medium containing a fiber base material, water, and a nutrient source. Hereinafter, the present invention will be described in detail.
栽培対象となる茸
本発明に用いることができる茸の種類については、特に制限されず、例えば、ヒラタケ、エノキタケ、エリンギ、タモギタケ、ブナシメジ、ナメコ、ハタケシメジ、シイタケ、マイタケ等が挙げられる。これらの中でも、ヒラタケ、シイタケ、エノキタケ、エリンギ、タモギタケ、ブナシメジ、シイタケ、マイタケは、本発明の栽培方法の適用対象茸として好適である。また、本発明の栽培方法は、従来公知の茸のみならず、将来、新たに発見又は作出される茸に対しても適用することができる。There are no particular restrictions on the type of koji that can be used in the present invention to be cultivated , and examples include oyster mushrooms, enokitake mushrooms, eringi, tamagotake mushrooms, bunashimeji, nameko, hayashi shimeji mushrooms, maitake mushrooms, and the like. Among these, oyster mushrooms, shiitake mushrooms, enokitake mushrooms, eringgi, tamogitake mushrooms, beech shimeji mushrooms, shiitake mushrooms, and maitake mushrooms are suitable as application target pods of the cultivation method of the present invention. Moreover, the cultivation method of this invention can be applied not only to a conventionally well-known cocoon but also to the cocoon newly discovered or produced in the future.
栽培用培地
本発明において、再利用可能な繊維基材、水、及び栄養源を含む栽培用培地を使用する。このように、栽培用培地の培養基材として、繊維基材を使用することにより、おが屑を使用せずとも、茸の菌床栽培が実現される。 Cultivation Medium In the present invention, a cultivation medium containing a reusable fiber substrate, water, and a nutrient source is used. Thus, by using a fiber base material as a culture base material for a culture medium, fungal bed culture of strawberries is realized without using sawdust.
本発明において、再利用可能な繊維基材とは、繊維から形成されている線状又はシート状の基材であり、茸栽培用培地の培養基材として一度使用しても、一般的な家庭用洗濯機又は商業用の洗濯機等で洗浄して再利用することができるものを指す。このような繊維基材として、具体的には、糸、縄、紐、綱等の線状繊維基材；不織布、織物、編物、フェルト等のシート状繊維基材が例示される。これらの中でも、不織布、織物、編物、フェルト等のシート状繊維基材は、栽培後の回収、洗浄が容易であるため、本発明において好適に使用される。 In the present invention, the reusable fiber base material is a linear or sheet base material formed from fibers, and even if it is used once as a culture base material for straw cultivation medium, It can be washed and reused in an industrial washing machine or a commercial washing machine. Specific examples of such fiber base materials include linear fiber base materials such as yarns, ropes, strings, and ropes; and sheet-like fiber base materials such as nonwoven fabrics, woven fabrics, knitted fabrics, and felts. Among these, sheet-like fiber substrates such as nonwoven fabrics, woven fabrics, knitted fabrics, and felts are preferably used in the present invention because they can be easily collected and washed after cultivation.
再利用可能な繊維基材を構成する繊維については、特に制限されず、天然繊維又は化学繊維の別を問わない。繊維基材を構成できる天然繊維の具体例としては、木綿、麻、羊毛、絹、カシミア等が挙げられる。また、繊維基材を構成できる化学繊維の具体例としては、ポリエチレンテレフタレート、ポリブチレンテレフタレート等のポリエステル、ナイロン６、ナイロン６，６等のポリアミド、ポリアクリロニトリル等のアクリル、ポリエチレン、ポリプロピレン等のポリオレフィン、ポリウレタン等の合成繊維；レーヨン、キュプラ、ポリノジック等の再生繊維；アセテート、プロミックス等の半合成繊維が挙げられる。これらの繊維は、１種単独で使用してもよく、また２種以上を組み合わせて使用してもよい。 The fiber constituting the reusable fiber substrate is not particularly limited, and may be natural fiber or chemical fiber. Specific examples of natural fibers that can constitute the fiber substrate include cotton, hemp, wool, silk, and cashmere. Specific examples of chemical fibers that can constitute the fiber substrate include polyesters such as polyethylene terephthalate and polybutylene terephthalate, polyamides such as nylon 6 and nylon 6,6, acrylics such as polyacrylonitrile, polyolefins such as polyethylene and polypropylene, Synthetic fibers such as polyurethane; regenerated fibers such as rayon, cupra and polynosic; semisynthetic fibers such as acetate and promix. These fibers may be used individually by 1 type, and may be used in combination of 2 or more type.
前記繊維基材は、栽培用培地において保水材としての役割も果たすので、本発明では、保水能力が高いものを使用することが望ましい。 Since the fiber base material also plays a role as a water retention material in the cultivation medium, it is desirable to use a material having a high water retention capacity in the present invention.
本発明で使用される栽培用培地には、水が含まれる。栽培用培地の調製に使用される水としては、特に制限されず、例えば、水道水、イオン交換水、蒸留水、超純水等のいずれであってもよい。 The cultivation medium used in the present invention contains water. It does not restrict | limit especially as water used for preparation of the culture medium for cultivation, For example, any of tap water, ion-exchange water, distilled water, ultrapure water, etc. may be sufficient.
栽培用培地中の水の含量については、使用する繊維基材の種類や量等に応じて適宜設定される。例えば、繊維基材１００ｇ当たり、水が１２０〜３６０ｇ程度となる範囲で繊維基材の種類に応じて適宜設定すればよい。 About the content of the water in the culture medium for cultivation, it sets suitably according to the kind, quantity, etc. of the fiber base material to be used. For example, what is necessary is just to set suitably according to the kind of fiber base material in the range from which water becomes about 120-360g per 100g of fiber base materials.
また、本発明で使用される栽培用培地には、茸を生育させるのに必要とされる栄養源が含まれる。このような栄養源としては、例えば、米糠、ふすま、コーンブラン、オカラ、ビール酵母、ダイズ粕等の不溶性栄養源；これらの不溶性栄養源の水抽出物、スクロース、カザミノ酸、無機塩類、塩酸チアミン等の可溶性栄養源挙げられる。これらの栄養源は、１種単独で使用してもよく、また２種以上を組み合わせて使用してもよい。 Further, the culture medium used in the present invention contains a nutrient source required for growing cocoons. Examples of such nutrient sources include insoluble nutrient sources such as rice bran, bran, corn bran, okara, brewer's yeast, soybean meal, etc .; water extracts of these insoluble nutrient sources, sucrose, casamino acids, inorganic salts, thiamine hydrochloride And other soluble nutrient sources. These nutrient sources may be used alone or in combination of two or more.
前記可溶性栄養源の内、不溶性栄養源の水抽出物については、例えば、固形状栄養源１ｇ当たり水３〜１０ｍｌ程度を混合して、必要に応じて加熱（例えば、８０〜１３０℃程度で１０〜３０分間の加熱）し、抽出処理し、液体画分を回収することにより得ることができる。 About the water extract of the insoluble nutrient source among the soluble nutrient sources, for example, about 3 to 10 ml of water per 1 g of the solid nutrient source is mixed and heated as necessary (for example, about 10 at 80 to 130 ° C.). It can be obtained by heating for ~ 30 minutes), extracting, and collecting the liquid fraction.
栽培用培地中の栄養源の含量については、栽培対象となる茸の種類、使用する栄養源の種類等に応じて適宜設定すればよい。本発明において、不溶性栄養源の使用量を減らす程、栽培後に培養基材の再利用のための回収が容易になるため、不溶性栄養源の使用量は低量であることが好ましい。不溶性栄養源の使用量を低減する場合、不溶性栄養源の水抽出物を使用することにより、茸の生育に必要とされる栄養源を補うことができる。使用される栽培用培地において、可溶性栄養源と不溶性栄養源の添加比率については、使用する各栄養源の種類と菌糸の増殖具合等を勘案して、適宜設定すればよい。 What is necessary is just to set suitably about the content of the nutrient source in the culture medium for cultivation according to the kind of cultivation target, the kind of nutrient source to be used, etc. In this invention, since the collection | recovery for reuse of a culture | cultivation base material becomes easy after cultivation, so that the usage-amount of an insoluble nutrient source is reduced, it is preferable that the usage-amount of an insoluble nutrient source is low. When reducing the amount of insoluble nutrient source to be used, the nutrient source required for the growth of the cocoon can be supplemented by using an aqueous extract of the insoluble nutrient source. In the cultivation medium to be used, the addition ratio of the soluble nutrient source and the insoluble nutrient source may be appropriately set in consideration of the type of each nutrient source to be used and the degree of growth of mycelia.
栽培方法
本発明の茸の栽培方法は、前記栽培用培地を用いて、茸を菌床栽培することにより行われる。茸の菌床栽培の方法としては、ビン栽培、袋栽培、箱栽培等のいずれであってもよいが、好ましくはビン栽培、袋栽培が挙げられる。本発明において、前記栽培用培地を用いること以外は、ビン栽培、袋栽培、箱栽培等の菌床栽培の条件は、通常の茸の菌床栽培と同様である。 Cultivation method The cultivation method of the koji of the present invention is carried out by cultivating the mushroom bed using the cultivation medium. As a method for cultivating the fungus bed of koji, any of bottle cultivation, bag cultivation, box cultivation and the like may be used, and preferably bottle cultivation and bag cultivation are mentioned. In this invention, except using the said culture medium, the conditions of fungal bed cultivation, such as bottle cultivation, bag cultivation, and box cultivation, are the same as that of normal fungal bed cultivation.
以下、例としてビン栽培及び袋栽培を挙げて、本発明の茸の栽培方法を実施する方法について説明する。 Hereinafter, a bottle cultivation and a bag cultivation are mentioned as an example, and the method of implementing the cultivation method of the straw of this invention is demonstrated.
［ビン栽培］
ビン栽培による菌床栽培は、ビン詰め、殺菌、接種、培養、必要に応じて菌掻き、芽出し、生育、収穫等の各工程を経て行われる。[Bin cultivation]
Bacteria bed cultivation by bottle cultivation is carried out through steps such as bottle stuffing, sterilization, inoculation, culture, and if necessary, bacteria scraping, budding, growth, and harvesting.
「ビン詰め」とは、前記栽培用培地を培養ビンに詰める工程である。ビン詰めは、予め調製した前記栽培用培地を培養ビンに詰めてもよく、また前記繊維基材を培養ビンに収容した後に、水及び栄養源の混合物を培養ビンに添加してもよい。培養ビンに収容する栽培用培地の量については、培養ビンの大きさに応じて適宜設定されるが、通常、栽培用培地が、培養ビンの容積の８０〜９５％を占めるように圧詰すればよい。 “Bottled” is a step of filling the culture medium in the culture bottle. In the bottle filling, the culture medium prepared in advance may be packed in a culture bottle, or the mixture of water and nutrients may be added to the culture bottle after the fiber base material is accommodated in the culture bottle. About the quantity of the culture medium accommodated in a culture bottle, it sets suitably according to the magnitude | size of a culture bottle, However, Usually, the culture medium is stuffed so that it may occupy 80 to 95% of the volume of a culture bottle. That's fine.
「殺菌」とは、培養ビンや栽培用培地の中に微生物を死滅させる工程である。殺菌は、通常、加熱殺菌法により行われる。殺菌の具体的条件として、１００〜１３０℃で１０〜３０分間が挙げられる。 “Sterilization” is a process of killing microorganisms in culture bottles or culture media. Sterilization is usually performed by a heat sterilization method. Specific conditions for sterilization include 10 to 30 minutes at 100 to 130 ° C.
「接種」とは、殺菌後に放冷した栽培用培地に、種菌を植え付ける工程である。種菌としては、液体培地で培養して得られた液体種菌、寒天培地で培養して得られた固体種菌のいずれを使用してもよい。種菌の接種量については、一般的なビン栽培の場合と同様である。 “Inoculation” is a process of planting inoculum into a culture medium that is allowed to cool after sterilization. As the inoculum, either a liquid inoculum obtained by culturing in a liquid medium or a solid inoculum obtained by culturing on an agar medium may be used. The inoculation amount of the inoculum is the same as in the case of general bottle cultivation.
「培養」とは、菌糸を蔓延させて成熟させる工程である。培養の条件については、使用する茸の種類、培養ビンの大きさ等に応じて適宜設定されるが、通常、２５℃、暗黒の条件で８〜３６日間程度が挙げられる。より具体的には、２５℃、暗黒の条件下で、ヒラタケであれば約８〜３６日、エノキタケであれば約１３〜３６日、エリンギであれば約１０〜３１日、タモギタケであれば約９〜１６日、ブナシメジであれば約１４〜３３日が挙げられる。なお、培養時の相対湿度は瓶のフタを閉めた状態で行うため、ほぼ１００％である。 “Cultivation” is a process in which hyphae are spread and matured. The culture conditions are appropriately set according to the type of koji used, the size of the culture bottle, and the like, and usually include about 8 to 36 days at 25 ° C. under dark conditions. More specifically, at 25 ° C. in the dark, about 8 to 36 days for oyster mushrooms, about 13 to 36 days for enokitake mushrooms, about 10 to 31 days for eringi, about 10 to 31 days for tamogitake If it is 9-16 days, it will be about 14-33 days for Bunashimeji. Note that the relative humidity during culture is approximately 100% because the bottle lid is closed.
「菌掻き」とは、必要に応じて行なわれる工程であり、種菌部分と培養基表面をかき取る工程である。 The “bacteria scraping” is a process performed as necessary, and is a process of scraping off the inoculum part and the culture medium surface.
「芽出し」とは、子実体原基や幼子実体を形成・生育させていく工程である。芽出しの条件については、使用する茸の種類に応じて適宜設定されるが、通常、１０〜１０００ルクス、１５〜１８℃の条件で３〜３０日間程度が挙げられる。より具体的には、１０〜１０００ルクス、１５〜１８℃の条件で、ヒラタケであれば約７〜１５日、エノキタケであれば約１２〜１９日、エリンギであれば約１１〜１８日、タモギタケであれば約３〜１１日、ブナシメジであれば約１８〜３０日が挙げられる。なお、芽出し時の相対湿度は瓶のフタを閉めた状態で行うため、ほぼ１００％である。 “Sprouting” is a process of forming and growing fruit body primordia and baby fruit body. About the conditions of budding, although it sets suitably according to the kind of koji to be used, about 3 to 30 days are normally mentioned on the conditions of 10-1000 lux and 15-18 degreeC. More specifically, under conditions of 10 to 1000 lux and 15 to 18 ° C., about 7 to 15 days for oyster mushroom, about 12 to 19 days for enokitake, about 11 to 18 days for eringi, About 3 to 11 days, and about 18 to 30 days for Bunashimeji. The relative humidity at the time of sprouting is almost 100% because it is performed with the bottle lid closed.
「生育」とは、子実体原基や幼子実体を収穫可能な成熟子実体に生育させる工程である。また、菌掻き後又は芽出し中に、必要に応じて水を栽培用培地に注水することにより、培地中の水分量の調整を行ってもよい。生育の条件については、使用する茸の種類、培養ビンの大きさ等に応じて適宜設定されるが、通常、１０〜１０００ルクス、１５〜１８℃の条件で６〜１６日間程度が挙げられる。より具体的には、１０〜１０００ルクス、１５〜１８℃の条件で、ヒラタケであれば約６〜８日、エノキタケであれば約９〜１１日、エリンギであれば約９〜１１日、タモギタケであれば約６〜８日、ブナシメジであれば約１２〜１６日が挙げられる。なお、生育時の相対湿度は瓶のフタを閉めた状態で行うため、ほぼ１００％である。 “Growth” is a process of growing a fruit body primordium or a young fruit body into a harvestable mature fruit body. Moreover, you may adjust the moisture content in a culture medium by pouring water into the culture medium as needed after scraping a fungus or during germination. The growth conditions are appropriately set according to the type of koji used, the size of the culture bottle, and the like, and usually include about 6 to 16 days under conditions of 10 to 1000 lux and 15 to 18 ° C. More specifically, under conditions of 10 to 1000 lux, 15 to 18 ° C., about 6 to 8 days for oyster mushrooms, about 9 to 11 days for enokitake, about 9 to 11 days for eringi, If so, about 6 to 8 days, and if Buna-Shimeji, about 12 to 16 days may be mentioned. The relative humidity during growth is almost 100% because the bottle lid is closed.
以上の工程を行うことにより、茸の成熟子実体を得ることができる。茸の成熟子実体を収穫すると、茸のビン栽培の全工程を終了する。 By performing the above steps, a mature fruit body of cocoon can be obtained. When the mature fruit body of the persimmon is harvested, the whole process of the persimmon bottle cultivation is completed.
ビン栽培に適した茸の種類としては、例えば、エノキタケ、ヒラタケ、エリンギ、タモギタケ、ブナシメジ等が挙げられる。 Examples of the types of koji suitable for bottle cultivation include enokitake, oyster mushrooms, eringi, tamogitake and bunashimeji.
［袋栽培］
袋栽培による菌床栽培は、袋詰め、殺菌、接種、培養、芽出し、生育、収穫等の各工程を経て行われる。[Bag cultivation]
Bacteria bed cultivation by bag cultivation is carried out through various processes such as bagging, sterilization, inoculation, culture, budding, growth, and harvesting.
「袋詰め」とは、前記栽培用培地を培養袋に詰める工程である。袋詰めは、前記繊維基材に水及び栄養源の混合物を含浸させた後に培養袋に詰めて行ってもよく、培養袋に繊維基材を詰めた後に水及び栄養源の混合物を含浸させてもよい。また、培養袋に詰める栽培用培地の量については、培養袋や繊維基材の大きさに応じて適宜設定され得るが、通常、培養袋の容積に対して４２〜７１％程度となるように調整すればよい。 “Packing” is a process of packing the cultivation medium in a culture bag. The bag filling may be performed by impregnating the fiber base material with a mixture of water and nutrient sources and then filling the culture bag, or after filling the culture bag with the fiber base material and impregnating the mixture of water and nutrient sources. Also good. In addition, the amount of the culture medium to be packed in the culture bag can be appropriately set according to the size of the culture bag or the fiber base material, but is usually about 42 to 71% with respect to the volume of the culture bag. Adjust it.
「殺菌」及び「接種」については、前記ビン栽培において記載される通りである。なお、「接種」工程における種菌の接種量については、一般的な袋栽培の場合と同様である。 "Sterilization" and "inoculation" are as described in the bottle cultivation. In addition, the inoculation amount of the inoculum in the “inoculation” step is the same as in the case of general bag cultivation.
「培養」についても前記ビン栽培の場合と同様の工程を指す。袋栽培の場合の培養の条件についても、使用する茸の種類、培養ビンの大きさ等に応じて適宜設定され、通常、２５℃、暗黒の条件で１３〜９０日間程度が挙げられる。より具体的には、２５℃、暗黒の条件下で、タモギタケであれば約１７〜２０日、ヒラタケであれば約１３〜１８日、シイタケであれば約９０日が挙げられる。なお、培養時の相対湿度は袋のフタを閉めた状態で行うため、ほぼ１００％である。 “Cultivation” refers to the same process as in the bottle cultivation. The culture conditions in the case of bag cultivation are also appropriately set according to the type of koji used, the size of the culture bottle, etc., and usually include about 13 to 90 days at 25 ° C. in the dark. More specifically, under conditions of 25 ° C. and dark, about 17 to 20 days for Tamogitake, about 13 to 18 days for Oyster mushroom, and about 90 days for Shiitake mushroom. The relative humidity during the culture is almost 100% because the bag is closed.
「芽出し」についても前記ビン栽培の場合と同様の工程を指す。袋栽培の場合の芽出しの条件についても、使用する茸の種類に応じて適宜設定され、通常、１０〜１０００ルクス、１５〜１８℃の条件で３〜１０日間程度が挙げられる。より具体的には、１０〜１０００ルクス、１５〜１８℃の条件で、タモギタケであれば約３〜７日、ヒラタケであれば約６〜１０日、シイタケであれば約５〜７日が挙げられる。なお、通常、芽出しは袋から培養基を取り出し、表面を露出させて行い、培養基への散水後、相対湿度が８０〜１００％の環境下で行われる。また、芽出し中に、必要に応じて水を栽培用培地に散水することにより、培地中の水分量の調整を行ってもよい。 “Sprouting” refers to the same process as in the bottle cultivation. The conditions for budding in the case of bag cultivation are also appropriately set according to the type of koji used, and usually include about 3 to 10 days under conditions of 10 to 1000 lux and 15 to 18 ° C. More specifically, under conditions of 10 to 1000 lux and 15 to 18 ° C., about 3 to 7 days for Tamogitake, about 6 to 10 days for Oyster mushrooms, about 5 to 7 days for Shiitake mushrooms It is done. In general, sprouting is performed by removing the culture medium from the bag and exposing the surface, and after watering the culture medium, the germination is performed in an environment where the relative humidity is 80 to 100%. Moreover, you may adjust the moisture content in a culture medium by sprinkling water to the culture medium for cultivation as needed.
「生育」についても前記ビン栽培の場合と同様の工程を指す。袋栽培の場合の生育の条件についても、使用する茸の種類、培養ビンの大きさ等に応じて適宜設定され、通常、１０〜１０００ルクス、１５〜１８℃の条件で７〜１０日間程度が挙げられる。より具体的には、１０〜１０００ルクス、１５〜１８℃の条件で、タモギタケであれば約７〜９日、ヒラタケであれば約７〜１０日、シイタケであれば約７〜９日が挙げられる。なお、生育は、通常、袋から培養基を取り出し、表面を露出させた状態で行い、相対湿度が８０〜１００％の環境下で行われる。また、生育中に、必要に応じて水を栽培用培地に散水することにより、培地中の水分量の調整を行ってもよい。 “Growth” refers to the same process as in the case of bottle cultivation. The conditions for growth in the case of bag cultivation are also set as appropriate according to the type of koji used, the size of the culture bottle, etc., and usually about 7 to 10 days under conditions of 10 to 1000 lux and 15 to 18 ° C. Can be mentioned. More specifically, under conditions of 10 to 1000 lux and 15 to 18 ° C., about 7 to 9 days for Tamotake, about 7 to 10 days for oyster mushroom, and about 7 to 9 days for shiitake mushroom. It is done. The growth is usually performed in a state where the culture medium is taken out of the bag and the surface is exposed, and the relative humidity is 80 to 100%. Moreover, you may adjust the moisture content in a culture medium by sprinkling water to the culture medium for cultivation as needed during growth.
以上の工程を行うことにより、茸の成熟子実体を得ることができる。茸の成熟子実体を収穫すると、茸の袋栽培の全工程を終了する。 By performing the above steps, a mature fruit body of cocoon can be obtained. When the mature fruit body of the cocoon is harvested, the whole process of bag cultivation of the cocoon is completed.
袋栽培に適した茸の種類としては、シイタケ、タモギタケ、ヒラタケ、マイタケが挙げられ、中でもシイタケは菌糸の成長方向が一定ではないため袋栽培の対象となる茸として好適である。 Examples of the types of koji suitable for bag cultivation include shiitake mushrooms, mushrooms, oyster mushrooms, and maitake mushrooms. Among these mushrooms, shiitake mushrooms are suitable for bag cultivation because the hyphal growth direction is not constant.
また、いずれの栽培方法においても茸の栽培後には、使用後の培地から回収し、洗浄された繊維基材は、再度、培養基材として再利用することができる。繊維基材は、使用後からの回収が容易であり、洗濯機等の既存の洗浄装置で簡便に洗浄することできる。 In any cultivation method, after cultivation of straw, the fiber base material recovered from the used medium and washed can be reused as a culture base material again. The fiber base material can be easily collected after use, and can be easily washed with an existing washing apparatus such as a washing machine.
以下、本発明を実施例により具体的に説明するが、本発明は以下の実施例の範囲のみに限定されるものではない。 EXAMPLES Hereinafter, although an Example demonstrates this invention concretely, this invention is not limited only to the range of a following example.
実施例１：エノキタケのビン栽培
１．培養液の調製
米糠５０ｇに水３００ｍｌを加え、１２１℃１０分で高温高圧滅菌を行い、放冷後、ガーゼを用いて液体画分を取り出し、米糠の熱水抽出物を得た。 Example 1: Enokitake Bin Cultivation Preparation of culture solution 300 ml of water was added to 50 g of rice bran, sterilized at high temperature and high pressure at 121 ° C. for 10 minutes, allowed to cool, and then the liquid fraction was taken out using gauze to obtain a hot water extract of rice bran.
また、別途、スクロース１０ｇ／Ｌ、カザミノ酸１０ｇ／Ｌ、ムラシゲ・スクーグ混合塩類粉末５ｇ／Ｌ、及び塩酸チアミン１０μｇ／Ｌを含む栄養補給液（残部は水）を調製した。 Separately, a nutrient replenisher solution (with the remainder being water) containing 10 g / L of sucrose, 10 g / L of casamino acid, 5 g / L of Murashige-Skoog mixed salt powder, and 10 μg / L of thiamine hydrochloride was prepared.
前記で得られた米糠の熱水抽出物３０ｍＬと栄養補給液３０ｍＬを混合し、更にこの混合液６０ｍＬに黒糖を０．６ｇ（最終濃度が１重量％）添加して混合することにより、培養液を調製した。 By mixing 30 mL of the rice bran hot water extract obtained above and 30 mL of the nutrient replenisher, and further adding 0.6 g (final concentration is 1% by weight) of brown sugar to this mixed solution, the culture solution is obtained. Was prepared.
２．栽培用培地の調製
２００ｍＬ容の培養ビン（内径４．５ｃｍ、表１に示す条件１〜８の場合）又は５００ｍＬ容の培養ビン（内径８．０ｃｍ、表１に示す条件９の場合）に、表１に示す各繊維基材を表１に示す形状に折り畳んで詰め込み、その上から米糠２ｇを添加した。次いで、上記で調整した培養液を表１に示す量添加し、ポリプロピレン製のネジ口蓋をした。これを１２１℃で１５分間、高温高圧滅菌することにより、栽培用培地を調製した。なお、条件８及び９で使用したおしぼり１枚当たりに添加する培養液の量は６０〜１００ｍｌが最適な範囲であることが確認できている。2. Preparation of culture medium In a 200 mL culture bottle (inner diameter 4.5 cm, conditions 1-8 shown in Table 1) or a 500 mL culture bottle (inner diameter 8.0 cm, conditions 9 shown in Table 1), Each fiber base material shown in Table 1 was folded and packed into the shape shown in Table 1, and 2 g of rice bran was added from above. Next, the amount of the culture solution prepared above was added as shown in Table 1, and a polypropylene screw cap was formed. This was cultivated at 121 ° C. for 15 minutes at high temperature and high pressure to prepare a culture medium. It has been confirmed that the optimal amount of the culture solution added per towel used in conditions 8 and 9 is 60 to 100 ml.
３．エノキタケの栽培
エノキタケの保存菌株を、ポテトデキストロース寒天培地（ＰＤＡ培地）を含む直径９０ｍｍのシャーレ上で、２５℃の暗黒条件下で１４日間培養させたものを種菌とした。生育した菌糸を直径３ｍｍのコルクボーラーで打ち抜き、前記で調製した栽培用培地の表面に１０個植菌した。２５℃、暗黒条件下で所定期間培養（表１）して、菌糸を培地全体に蔓延させた。3. Cultivation of Enokitake A stock of Enokitake was cultured on a petri dish with a diameter of 90 mm containing potato dextrose agar medium (PDA medium) under dark conditions at 25 ° C. for 14 days as an inoculum. The grown mycelium was punched with a cork borer having a diameter of 3 mm, and 10 cells were inoculated on the surface of the culture medium prepared above. Culture was performed for a predetermined period of time at 25 ° C. under dark conditions (Table 1), and the mycelium was spread throughout the medium.
その後、菌掻き作業を行い、培地表面が湿る程度に水を注水した。メンブレン付きのフタに代えて１５℃、３００ｌｕｘの光条件下において所定期間培養（表１）をすると、子実体原基を確認した。子実体原基の形成確認後に、同条件で更に約１０日間培養すると、成熟した子実体が得られた。 Then, the fungus scraping operation was performed, and water was poured to such an extent that the surface of the culture medium became wet. When culturing for a predetermined period (Table 1) under light conditions of 15 ° C. and 300 lux instead of the lid with the membrane, the fruiting body primordium was confirmed. After confirming the formation of the fruiting body primordium, when cultured for about 10 days under the same conditions, a mature fruiting body was obtained.
表１にエノキタケの栽培結果を纏めて示す。また、各条件での栽培後のエノキタケの外観を観察した結果を図１及び２に示す。表１に示す繊維基材を使用した栽培結果は、おが屑２０ｇ、米糠１６ｇ及び水６５ｍＬを含む栽培用培地を２００ｍＬ容の培養ビンに詰めてエノキタケを栽培した場合と、生育速度、栽培日数、子実体の大きさや形状の点で同程度のものであった。 Table 1 summarizes the cultivation results of enokitake mushrooms. Moreover, the result of having observed the appearance of the enokitake mushroom after cultivation on each condition is shown in FIGS. The results of cultivation using the fiber base material shown in Table 1 are the growth rate, the number of days of cultivation, and the child, when the cultivation medium containing 20 g of sawdust, 16 g of rice bran, and 65 mL of water is packed in a 200 mL culture bottle and grown. It was about the same size in terms of size and shape.
実施例２：ヒラタケのビン栽培
１．培養液の調製
実施例１の場合と同様の方法で、培養液を調製した。 Example 2: Oyster mushroom bottle cultivation Preparation of culture solution A culture solution was prepared in the same manner as in Example 1.
２．栽培用培地の調製
２００ｍＬ容の培養ビン（内径４．５ｃｍ、表２に示す条件１〜１０の場合）又は５００ｍＬ容の培養ビン（内径８．０ｃｍ、表２に示す条件１１の場合）に、表２に示す各繊維基材を表２に示す形状に折り畳んで詰め込み、その上から米糠２ｇを添加した。次いで、上記で調整した培養液を表２に示す量添加し、ポリプロピレン製のネジ口蓋をした。これを１２１℃で１５分間、高温高圧滅菌することにより、栽培用培地を調製した。なお、条件１０及び１１で使用したおしぼり１枚当たりに添加する培養液の量は６０〜１００ｍｌが最適な範囲であることが確認できている。2. Preparation of cultivation medium In a 200 mL culture bottle (inner diameter 4.5 cm, conditions 1 to 10 shown in Table 2) or a 500 mL culture bottle (inner diameter 8.0 cm, conditions 11 shown in Table 2), Each fiber base material shown in Table 2 was folded and packed into the shape shown in Table 2, and 2 g of rice bran was added from above. Subsequently, the culture solution prepared above was added in the amount shown in Table 2, and a screw cap made of polypropylene was used. This was cultivated at 121 ° C. for 15 minutes at high temperature and high pressure to prepare a culture medium. It has been confirmed that the amount of the culture solution added per towel used in conditions 10 and 11 is in an optimal range of 60 to 100 ml.
３．ヒラタケの栽培
ヒラタケの保存菌株を、ポテトデキストロース寒天培地（ＰＤＡ培地）を含む直径９０ｍｍのシャーレ上で、２５℃の暗黒条件下で１４日間培養させたものを種菌とした。生育した菌糸を直径３ｍｍのコルクボーラーで打ち抜き、前記で調製した栽培用培地の表面に１０個植菌した。２５℃、暗黒条件下で所定期間培養（表２）して、菌糸を培地全体に蔓延させた。3. Cultivation of oyster mushrooms A stock strain of oyster mushrooms cultured on a petri dish having a diameter of 90 mm containing potato dextrose agar medium (PDA medium) under dark conditions at 25 ° C. for 14 days was used as an inoculum. The grown mycelium was punched with a cork borer having a diameter of 3 mm, and 10 cells were inoculated on the surface of the culture medium prepared above. Culture was performed for a predetermined period of time at 25 ° C. under dark conditions (Table 2), and the mycelium was spread throughout the medium.
その後、菌掻き作業を行い、培地表面が湿る程度に水を注水した。メンブレン付きのフタに代えて１５℃、３００ｌｕｘの光条件下において所定期間培養（表２）をすると、子実体原基を確認した。子実体原基の形成確認後に、更に同条件で約７日間培養すると、成熟した子実体が得られた。 Then, the fungus scraping operation was performed, and water was poured to such an extent that the surface of the culture medium was moistened. When culturing for a predetermined period (Table 2) under a light condition of 15 ° C. and 300 lux instead of the lid with the membrane, the fruiting body primordium was confirmed. After confirming the formation of the fruiting body primordium, when further cultured for about 7 days under the same conditions, a mature fruiting body was obtained.
表２に各条件でヒラタケを栽培した結果を纏めて示す。また、条件１１での栽培後のヒラタケの外観を観察した結果を図３に示す。表２に示す繊維基材を使用した栽培結果は、おが屑２０ｇ、米糠約１６ｇ及び水６５ｍＬを含む栽培用培地を２００ｍＬ容の培養ビンに詰めてヒラタケを栽培した場合と、生育速度、栽培日数、子実体の大きさや形状は同程度のものであった。 Table 2 summarizes the results of cultivation of oyster mushrooms under each condition. Moreover, the result of having observed the appearance of the oyster mushroom after cultivation on condition 11 is shown in FIG. The cultivation results using the fiber base material shown in Table 2 are the growth rate, the number of days of cultivation, and the case where the cultivation medium containing sawdust 20 g, rice bran about 16 g and water 65 mL is packed in a 200 mL culture bottle and oyster mushrooms are cultivated. The size and shape of the fruiting bodies were similar.
実施例３：エリンギのビン栽培
１．培養液の調製
実施例１の場合と同様の方法で、培養液を調製した。 Example 3: Elingi bottle cultivation Preparation of culture solution A culture solution was prepared in the same manner as in Example 1.
２．栽培用培地の調製
２００ｍＬ容の培養ビン（内径４．５ｃｍ、表３に示す条件１〜９の場合）又は５００ｍＬ容の培養ビン（内径８．０ｃｍ、表３に示す条件１０の場合）に、表３に示す各繊維基材を表３に示す形状に折り畳んで詰め込み、その上から米糠２ｇを添加した。次いで、上記で調整した培養液を表３に示す量添加し、ポリプロピレン製のネジ口蓋をした。これを１２１℃で１５分間、高温高圧滅菌することにより、栽培用培地を調製した。なお、条件９及び１０で使用したおしぼり１枚当たりに添加する培養液の量は６０〜１００ｍｌが最適な範囲であることが確認できている。2. Preparation of cultivation medium In a 200 mL culture bottle (inner diameter 4.5 cm, conditions 1 to 9 shown in Table 3) or a 500 mL culture bottle (inner diameter 8.0 cm, conditions 10 shown in Table 3), Each fiber base material shown in Table 3 was folded and packed into the shape shown in Table 3, and 2 g of rice bran was added from above. Subsequently, the culture solution prepared above was added in the amount shown in Table 3, and a polypropylene screw cap was formed. This was cultivated at 121 ° C. for 15 minutes at high temperature and high pressure to prepare a culture medium. It has been confirmed that the optimal amount of the culture solution added per hand towel used in conditions 9 and 10 is in the range of 60 to 100 ml.
３．エリンギの栽培
エリンギの保存菌株を、ポテトデキストロース寒天培地（ＰＤＡ培地）を含む直径９０ｍｍのシャーレ上で、２５℃の暗黒条件下で１４日間培養させたものを種菌とした。生育した菌糸を直径３ｍｍのコルクボーラーで打ち抜き、前記で調製した栽培用培地の表面に１０個植菌した。２５℃、暗黒条件下で所定期間培養（表３）して、菌糸を培地全体に蔓延させた。3. Cultivation of eringi A stock strain of eringi was cultured on a petri dish having a diameter of 90 mm containing a potato dextrose agar medium (PDA medium) for 14 days under dark conditions at 25 ° C. as a seed. The grown mycelium was punched with a cork borer having a diameter of 3 mm, and 10 cells were inoculated on the surface of the culture medium prepared above. Culture was performed for a predetermined period of time under dark conditions at 25 ° C. (Table 3), and the mycelium was spread throughout the medium.
その後、菌掻き作業を行い、培地表面が湿る程度に水を注水した。メンブレン付きのフタに代えて１５℃、３００ｌｕｘの光条件下において所定期間培養（表３）をすると、子実体原基を確認した。子実体原基の形成確認後に、更に同条件で約１０日間培養すると、成熟した子実体が得られた。 Then, the fungus scraping operation was performed, and water was poured to such an extent that the surface of the culture medium became wet. When culturing for a predetermined period (Table 3) under a light condition of 15 ° C. and 300 lux instead of the lid with the membrane, the fruiting body primordium was confirmed. After confirming the formation of the fruiting body primordium, further culturing for about 10 days under the same conditions yielded a mature fruiting body.
表３に各条件でエリンギを栽培した結果を纏めて示す。また、条件９での栽培後のエリンギの外観を観察した結果を図４に示す。表３に示す繊維基材を使用した栽培結果は、おが屑２０ｇ、米糠１６ｇ及び水６５ｍＬを含む栽培用培地を２００ｍＬ容の培養ビンに詰めてエリンギを栽培した場合と生育速度、栽培日数、子実体の大きさや形状の点で、同程度のものであった。 Table 3 summarizes the results of cultivating eringi under each condition. Moreover, the result of having observed the external appearance of the eringi after cultivation on condition 9 is shown in FIG. The results of cultivation using the fiber base material shown in Table 3 are the growth rate, cultivation days, fruiting body, and the case where cultivation medium containing 20 g of sawdust, 16 g of rice bran, and 65 mL of water is packed in a 200 mL culture bottle and cultivated eringi. In terms of size and shape.
実施例４：タモギタケのビン栽培
１．培養液の調製
実施例１の場合と同様の方法で、培養液を調製した。 Example 4: Cultivation of Tamogitake bottles Preparation of culture solution A culture solution was prepared in the same manner as in Example 1.
２．栽培用培地の調製
２００ｍＬ容の培養ビン（内径４．５ｃｍ）に、表４に示す各繊維基材を表４に示す形状に折り畳んで詰め込み、その上から米糠２ｇを添加した。次いで、上記で調整した培養液を表４に示す量添加し、ポリプロピレン製のネジ口蓋をした。これを１２１℃で１５分間、高温高圧滅菌することにより、栽培用培地を調製した。なお、条件１０で使用したおしぼり１枚当たりに添加する培養液の量は６０〜１００ｍｌが最適な範囲であることが確認できている。2. Preparation of culture medium Each fiber substrate shown in Table 4 was folded and packed into a 200 mL culture bottle (inner diameter: 4.5 cm) in the shape shown in Table 4, and 2 g of rice bran was added thereto. Next, the amount of the culture solution prepared above was added as shown in Table 4 to form a screw cap made of polypropylene. This was cultivated at 121 ° C. for 15 minutes at high temperature and high pressure to prepare a culture medium. In addition, it has confirmed that the amount of the culture solution added per one hand towel used on the conditions 10 is the optimal range of 60-100 ml.
３．タモギタケの栽培
タモギタケの保存菌株を、ポテトデキストロース寒天培地（ＰＤＡ培地）を含む直径９０ｍｍのシャーレ上で、２５℃の暗黒条件下で１４日間培養させたものを種菌とした。生育した菌糸を直径３ｍｍのコルクボーラーで打ち抜き、前記で調製した栽培用培地の表面に１０個植菌した。２５℃、暗黒条件下で所定期間培養（表４）して、菌糸を培地全体に蔓延させた。3. Cultivation of Tamogitake A stock strain of Tamogitake was cultured on a petri dish having a diameter of 90 mm containing potato dextrose agar medium (PDA medium) under dark conditions at 25 ° C. for 14 days, and was used as an inoculum. The grown mycelium was punched with a cork borer having a diameter of 3 mm, and 10 cells were inoculated on the surface of the culture medium prepared above. Culture was performed for a predetermined period of time at 25 ° C. under dark conditions (Table 4), and the mycelium was spread throughout the medium.
その後、フタを外し、培地の上からビン一杯に蒸留水を加え、2時間の灌水作業を行った。２５℃、３００ｌｕｘの光条件下において所定期間培養（表４）すると、子実体原基を確認した。子実体原基の形成確認後に、更に同条件で約７日間培養すると、成熟した子実体が得られた。 Thereafter, the lid was removed, distilled water was added to the full bottle from the top of the medium, and watering was performed for 2 hours. When cultured for a predetermined period of time under light conditions of 25 ° C. and 300 lux (Table 4), fruiting body primordia were confirmed. After confirming the formation of the fruiting body primordium, when further cultured for about 7 days under the same conditions, a mature fruiting body was obtained.
表４に各条件でタモギタケを栽培した結果を纏めて示す。表４に示す繊維基材を使用した栽培結果は、おが屑２０ｇ、米糠１６ｇ及び水６５ｍＬを含む栽培用培地を２００ｍＬ容の培養ビンに詰めてタモギタケを栽培した場合と、生育速度、栽培日数、子実体の大きさや形状の点で、同程度のものであった。 Table 4 summarizes the results of cultivating Tamogitake under each condition. The cultivation results using the fiber substrate shown in Table 4 are the growth rate, the number of cultivation days, and the child, when cultivation medium containing 20 g of sawdust, 16 g of rice bran and 65 mL of water is packed in a 200 mL culture bottle and grown It was about the same size and shape.
実施例５：ブナシメジのビン栽培
１．培養液の調製
実施例１の場合と同様の方法で、培養液を調製した。 Example 5: Buna shimeji bottle cultivation Preparation of culture solution A culture solution was prepared in the same manner as in Example 1.
２．栽培用培地の調製
２００ｍＬ容の培養ビン（内径４．５ｃｍ）に、表５に示す各繊維基材を表５に示す形状に折り畳んで詰め込み、その上から米糠２ｇを添加した。次いで、上記で調整した培養液を表５に示す量添加し、ポリプロピレン製のネジ口蓋をした。これを１２１℃で１５分間、高温高圧滅菌することにより、栽培用培地を調製した。なお、条件５で使用したおしぼり１枚当たりに添加する培養液の量は６０〜１００ｍｌが最適な範囲であることが確認できている。2. Preparation of culture medium Each fiber base material shown in Table 5 was folded into a 200 mL culture bottle (inner diameter: 4.5 cm) and packed in the shape shown in Table 5, and 2 g of rice bran was added thereto. Next, the amount of the culture solution prepared above was added as shown in Table 5, and a screw cap made of polypropylene was used. This was cultivated at 121 ° C. for 15 minutes at high temperature and high pressure to prepare a culture medium. It has been confirmed that the optimal amount of the culture solution added per hand towel used in Condition 5 is 60 to 100 ml.
３．ブナシメジの栽培
ブナシメジの保存菌株を、ポテトデキストロース寒天培地（ＰＤＡ培地）を含む直径９０ｍｍのシャーレ上で、２５℃の暗黒条件下で２０日間培養させたものを種菌とした。生育した菌糸を直径３ｍｍのコルクボーラーで打ち抜き、前記で調製した栽培用培地の表面に１０個植菌した。２５℃、暗黒条件下で所定期間培養（表５）して、菌糸を培地全体に蔓延させた。3. Cultivation of Buna Shimeji A stock of Buna shimeji was cultured on a petri dish having a diameter of 90 mm containing a potato dextrose agar medium (PDA medium) under dark conditions at 25 ° C. for 20 days as a seed fungus. The grown mycelium was punched with a cork borer having a diameter of 3 mm, and 10 cells were inoculated on the surface of the culture medium prepared above. Culture was performed for a predetermined period of time at 25 ° C. under dark conditions (Table 5), and the mycelium was spread throughout the medium.
その後、菌掻き作業を行い、培地表面が湿る程度に水を注水した。メンブレン付きのフタに代えて１５℃、３００ｌｕｘの光条件下において所定期間培養（表５）をすると、子実体原基を確認した。子実体原基の形成確認後に、更に同条件で約１４日間培養すると、成熟した子実体が得られた。 Then, the fungus scraping operation was performed, and water was poured to such an extent that the surface of the culture medium became wet. When culturing for a predetermined period (Table 5) under light conditions of 15 ° C. and 300 lux instead of the lid with the membrane, the fruiting body primordium was confirmed. After confirming the formation of the fruiting body primordium, further culturing for about 14 days under the same conditions, a mature fruiting body was obtained.
表５に各条件でブナシメジを栽培した結果を纏めて示す。また、条件５での栽培後のブナシメジの外観を観察した結果を図５に示す。表５に示す繊維基材を使用した栽培結果は、おが屑２０ｇ、米糠１６ｇ及び水６５ｍＬを含む栽培用培地を２００ｍＬ容の培養ビンに詰めてブナシメジを栽培した場合と、生育速度、栽培日数、子実体の大きさや形状の点で、同程度のものであった。 Table 5 summarizes the results of cultivating Bunashimeji under each condition. Moreover, the result of having observed the appearance of the beech shimeji after cultivation on condition 5 is shown in FIG. The cultivation results using the fiber base materials shown in Table 5 are the growth rate, the number of cultivation days, and the child, when cultivating beech shimeji mushrooms in a 200 ml culture bottle containing 20 g of sawdust, 16 g of rice bran and 65 mL of water. It was about the same size and shape.
実施例６：タモギタケの袋栽培
１．培養液の調製
実施例１の場合と同様の方法で、培養液を調製した。 Example 6: Bag cultivation of Tamogitake Preparation of culture solution A culture solution was prepared in the same manner as in Example 1.
２．栽培用培地の調製
縦４５ｃｍ×横２０ｃｍの栽培用袋の中に、１枚につき６０〜１００ｍｌの培養液を浸み込ませた、縦２７ｃｍ×横２７ｃｍのおしぼり（綿）を二つ折りにした物を３枚、重ねて敷いた。重ねたおしぼりの間には、１０ｇの米ぬかをおしぼり全体に広がるように入れ、合計で２０ｇの米ぬかが入るようにした。その結果、栽培袋の中に占める繊維基材の体積は、約４２０ｃｍ3となった。これを、１２１℃、３０分で高温・高圧滅菌を行うことにより、栽培用培地を調製した。2. Preparation of cultivation medium Cultured bag of length 45cm x width 20cm soaked 60-100ml of culture solution per piece, folded into 27cm x 27cm width hand towel (cotton) Three sheets were laid on top of each other. Between the wet towels, 10 g of rice bran was placed so as to spread over the whole towel, so that 20 g of rice bran was added in total. As a result, the volume of the fiber base material in the cultivation bag was about 420 cm 3 . This was sterilized at 121 ° C. for 30 minutes at a high temperature and high pressure to prepare a culture medium for cultivation.
３．タモギタケの栽培
タモギタケの保存菌株を、ポテトデキストロース寒天培地（ＰＤＡ培地）を含む直径９０ｍｍのシャーレ上で、２５℃の暗黒条件下で１４日間培養させたものを種菌とした。生育した菌糸を直径３ｍｍのコルクボーラーで打ち抜き、前記で調製した栽培用培地の表面に３０個植菌した。２５℃、暗黒条件下で所定期間培養（表６）して、菌糸を培地全体に蔓延させた。3. Cultivation of Tamogitake A stock strain of Tamogitake was cultured on a petri dish having a diameter of 90 mm containing potato dextrose agar medium (PDA medium) under dark conditions at 25 ° C. for 14 days, and was used as an inoculum. The grown mycelium was punched with a cork borer having a diameter of 3 mm, and 30 cells were inoculated on the surface of the culture medium prepared above. Culture was performed for a predetermined period of time at 25 ° C. under dark conditions (Table 6), and the mycelium was spread throughout the medium.
その後、袋から菌糸が蔓延したおしぼりを取り出し、プラスチック製のタッパーの中に入れ、霧吹きで水を噴霧した後、１５℃、３００ｌｕｘの光条件下において所定期間培養（表６）すると、子実体原基を確認した。子実体原基の形成確認後に、更に同条件で約７日間培養すると、成熟した子実体が得られた。 Thereafter, a hand towel with a spread of hyphae is taken out from the bag, placed in a plastic tapper, sprayed with water by spraying, and then cultured for a predetermined period of time under light conditions of 15 ° C. and 300 lux (Table 6). The group was confirmed. After confirming the formation of the fruiting body primordium, when further cultured for about 7 days under the same conditions, a mature fruiting body was obtained.
実施例７：ヒラタケの袋栽培
１．培養液の調製
実施例１の場合と同様の方法で、培養液を調製した。 Example 7: Oyster mushroom bag cultivation Preparation of culture solution A culture solution was prepared in the same manner as in Example 1.
２．栽培用培地の調製
縦４５ｃｍ×横２０ｃｍの栽培用袋の中に、１枚につき６０〜１００ｍｌの培養液を浸み込ませた、縦２７ｃｍ×横２７ｃｍのおしぼり（綿）を二つ折りにした物を３枚、重ねて敷いた。重ねたおしぼりの間には、１０ｇの米ぬかをおしぼり全体に広がるように入れ、合計で２０ｇの米ぬかが入るように入れた。その結果、栽培袋の中に占める繊維基材の体積は、約４２０ｃｍ3となった。その後、１２１℃、３０分で高温・高圧滅菌を行うことにより、栽培用培地を調製した。2. Preparation of cultivation medium Cultured bag of length 45cm x width 20cm soaked 60-100ml of culture solution per piece, folded into 27cm x 27cm width hand towel (cotton) Three sheets were laid on top of each other. Between the wet towels, 10 g of rice bran was put so as to spread over the whole wet towel, and 20 g of rice bran was put in total. As a result, the volume of the fiber base material in the cultivation bag was about 420 cm 3 . Then, the culture medium for cultivation was prepared by performing high temperature and high pressure sterilization at 121 degreeC for 30 minutes.
３．ヒラタケの栽培
ヒラタケの保存菌株を、ポテトデキストロース寒天培地（ＰＤＡ培地）を含む直径９０ｍｍのシャーレ上で、２５℃の暗黒条件下で１４日間培養させたものを種菌とした。生育した菌糸を直径３ｍｍのコルクボーラーで打ち抜き、前記で調製した栽培用培地の表面に３０個植菌した。２５℃、暗黒条件下で所定期間培養（表７）して、菌糸を培地全体に蔓
延させた。3. Cultivation of oyster mushrooms A stock strain of oyster mushrooms cultured on a petri dish having a diameter of 90 mm containing potato dextrose agar medium (PDA medium) under dark conditions at 25 ° C. for 14 days was used as an inoculum. The grown mycelium was punched with a cork borer having a diameter of 3 mm, and 30 cells were inoculated on the surface of the culture medium prepared above. Culture was performed for a predetermined period of time at 25 ° C. under dark conditions (Table 7), and the mycelium was spread throughout the medium.
その後、袋から菌糸が蔓延したおしぼりを取り出し、プラスチック製のタッパーの中に入れ、霧吹きで水を噴霧した後に１５℃、３００ｌｕｘの光条件下において所定期間培養（表７）すると、子実体原基を確認した。子実体原基の形成確認後に、更に同条件で約７日間培養すると、成熟した子実体が得られた。 After that, the hand towel in which the mycelium has spread is taken out from the bag, put into a plastic tapper, sprayed with water by spraying, and then cultured at 15 ° C. under a light condition of 300 lux for a predetermined period (Table 7). It was confirmed. After confirming the formation of the fruiting body primordium, when further cultured for about 7 days under the same conditions, a mature fruiting body was obtained.
実施例８：シイタケの袋栽培
１．培養液の調製
実施例１の場合と同様の方法で、培養液を調製した。 Example 8: Bag cultivation of shiitake mushrooms Preparation of culture solution A culture solution was prepared in the same manner as in Example 1.
２．栽培用培地の調製
縦４５ｃｍ×横２０ｃｍの栽培用袋の中に、１枚につき６０〜１００ｍｌの培養液を浸み込ませた、縦２７ｃｍ×横２７ｃｍのおしぼりを二つ折りにした物を４枚、重ねて敷いた。重ねたおしぼりの間には、１０ｇの米ぬかをおしぼり全体に広がるように入れ、合計で３０ｇの米ぬかが入るように入れた。その結果、栽培袋の中に占める繊維基材の体積は、約５６０ｃｍ3となった。その後、１２１℃、３０分で高温・高圧滅菌を行うことにより、栽培用培地を調製した。2. Preparation of cultivation medium 4 pieces of folds of 27cm x 27cm horizontal hand towel soaked with 60-100ml culture medium in a 45cm long x 20cm wide cultivation bag Laid on top of each other. Between the wet towels, 10 g of rice bran was put so as to spread over the whole wet towel, and a total of 30 g of rice bran was put. As a result, the volume of the fiber base material in the cultivation bag was about 560 cm 3 . Then, the culture medium for cultivation was prepared by performing high temperature and high pressure sterilization at 121 degreeC for 30 minutes.
３．シイタケの栽培
シイタケの保存菌株を、ポテトデキストロース寒天培地（ＰＤＡ培地）を含む直径９０ｍｍのシャーレ上で、２５℃の暗黒条件下で１４日間培養させたものを種菌とした。生育した菌糸を直径３ｍｍのコルクボーラーで打ち抜き、前記で調製した栽培用培地の表面に３０個植菌した。２５℃、暗黒条件下で所定期間培養（表８）して、菌糸を培地全体に蔓延させた。3. Cultivation of shiitake mushrooms A stock strain of shiitake mushrooms was cultured on a petri dish having a diameter of 90 mm containing potato dextrose agar medium (PDA medium) under dark conditions at 25 ° C. for 14 days as an inoculum. The grown mycelium was punched with a cork borer having a diameter of 3 mm, and 30 cells were inoculated on the surface of the culture medium prepared above. Culture was performed for a predetermined period of time at 25 ° C. under dark conditions (Table 8), and the mycelium was spread throughout the medium.
その後、袋から菌糸が蔓延したおしぼりを取り出し、プラスチック製のタッパーの中に入れ、霧吹きで水を噴霧した後に１５℃、３００ｌｕｘの光条件下において所定期間培養（表８）すると、子実体原基を確認した。子実体原基の形成確認後に、更に同条件で約７日間培養すると、成熟した子実体が得られた。 Thereafter, a hand towel in which hyphae have spread is taken out from the bag, placed in a plastic tapper, sprayed with water by spraying, and then cultured under a light condition of 15 ° C. and 300 lux for a predetermined period of time (Table 8). It was confirmed. After confirming the formation of the fruiting body primordium, when further cultured for about 7 days under the same conditions, a mature fruiting body was obtained.
Claims (12)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2012157757 | 2012-07-13 | ||
| JP2012157757 | 2012-07-13 | ||
| JP2013016095 | 2013-01-30 | ||
| JP2013016095 | 2013-01-30 | ||
| PCT/JP2013/064210 WO2014010314A1 (en) | 2012-07-13 | 2013-05-22 | Method for cultivating mushrooms using reusable fiber substrate, and culture medium for cultivation using same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPWO2014010314A1 JPWO2014010314A1 (en) | 2016-06-20 |
| JP6085889B2 true JP6085889B2 (en) | 2017-03-01 |
Family
ID=49915788
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014524685A Expired - Fee Related JP6085889B2 (en) | 2012-07-13 | 2013-05-22 | Straw cultivation method using reusable fiber as a base material, and cultivation medium used therefor |
Country Status (3)
| Country | Link |
|---|---|
| JP (1) | JP6085889B2 (en) |
| TW (1) | TW201402001A (en) |
| WO (1) | WO2014010314A1 (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5704671B1 (en) * | 2014-09-11 | 2015-04-22 | 合同会社クリエーション | How to grow tamogitake |
| CN104628471A (en) * | 2015-02-02 | 2015-05-20 | 邬方成 | Preparation method of pleurotus eryngii cultivation material |
| CN104744151A (en) * | 2015-03-06 | 2015-07-01 | 邬方成 | Method for manufacturing pleurotus eryngii compost |
| CN104744150A (en) * | 2015-03-06 | 2015-07-01 | 邬方成 | Method for manufacturing pleurotus eryngii compost |
| CN104744149A (en) * | 2015-03-06 | 2015-07-01 | 邬方成 | Method for manufacturing pleurotus eryngii compost |
| JP6517640B2 (en) * | 2015-09-08 | 2019-05-22 | 仁実サポート 株式会社 | Cultivation method of Bunashiji |
| ITUA20162732A1 (en) * | 2016-04-20 | 2017-10-20 | Mario Alfredo Antonio Castelluccio | PROCEDURE FOR CULTIVATION OF THE MUSHROOM MUSHROOM IN A CLIMATE AND STERILE ENVIRONMENT |
| NL2022282B1 (en) * | 2018-12-21 | 2020-07-21 | Rijk Zwaan Zaadteelt En Zaadhandel Bv | Substrate for producing consumer-ready vegetables, mushrooms or herbs in a closed container |
| CN111802170A (en) * | 2019-04-10 | 2020-10-23 | 四季洋圃生物机电股份有限公司 | Environment-friendly liquid cultivation method of mushroom |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3806225B2 (en) * | 1997-05-26 | 2006-08-09 | ホクト株式会社 | Method for producing mushroom medium |
| JP2003009656A (en) * | 2001-06-28 | 2003-01-14 | Akita Jujo Kasei Kk | Method for culturing mushrooms |
| JP3533200B2 (en) * | 2001-11-05 | 2004-05-31 | 博一 藤澤 | Mushroom cultivation method |
| JP2003310050A (en) * | 2002-04-26 | 2003-11-05 | Poran:Kk | Medium and method for culturing mushroom |
| JP2011078394A (en) * | 2009-10-06 | 2011-04-21 | Kimio Munemura | Fermentation medium for edible mushroom and production method therefor |
-
2013
- 2013-05-22 JP JP2014524685A patent/JP6085889B2/en not_active Expired - Fee Related
- 2013-05-22 WO PCT/JP2013/064210 patent/WO2014010314A1/en active Application Filing
- 2013-05-24 TW TW102118433A patent/TW201402001A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO2014010314A1 (en) | 2014-01-16 |
| JPWO2014010314A1 (en) | 2016-06-20 |
| TW201402001A (en) | 2014-01-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP6085889B2 (en) | Straw cultivation method using reusable fiber as a base material, and cultivation medium used therefor | |
| JP6548124B2 (en) | A culture solution for mulberry cultivation, a culture medium for mulberry cultivation, a method for producing a culture liquid for mulberry cultivation, and a cultivation method for mulberry | |
| CN102405843B (en) | Seedling method for tissue culture and rapid breeding of camellia semiserrata chi | |
| CN106818207B (en) | A kind of bag cultivation growing straight method of needle mushroom | |
| CN103875717B (en) | Method for promoting orchid to grow and preventing diseases | |
| CN104322276A (en) | Method for improving preparing efficiency and yield and quality of shiitake sticks | |
| CN102612966B (en) | Method for obtaining dendrobium aphyllum seeds to germinate effective symbiotic fungi | |
| CN107711290A (en) | The culture medium and its synchronous cultural method of mycorhiza edible mushroom symbiosis seedling | |
| CN104541938A (en) | Merge-cultivating method of Ganoderma | |
| CN108207487A (en) | A kind of cultural method of agaric | |
| CN103749154A (en) | Culture medium for promoting cross infection among truffle mycorrhizas and using method thereof | |
| CN108094051A (en) | A kind of Coriaria sinica mushroom strain culture medium and preparation method thereof and the method for cultivating Coriaria sinica mushroom | |
| CN106879557A (en) | A kind of method for raising black striped plant bug | |
| CN101914474B (en) | Ectomycorrhizal helper bacteria bacillus pumilus and application thereof | |
| CN104273176B (en) | A kind of plant organic matter-bacillus subtilis mix bacterium agent, preparation method and application | |
| CN104285677B (en) | A kind of preparation method of edible mushroom peg wood bacterial classification | |
| CN103571788B (en) | A kind of thecasporous Collection and preservation method of Cordyceps militaris (L.) Link. | |
| CN109845516B (en) | Device and method for breeding blueberry specific symbiotic mycorrhizal fungi | |
| CN102599014A (en) | Method for artificially inducing field wheat scab | |
| CN106834143A (en) | Microbial bacterial agent, green garbage soil-repairing agent and its application | |
| CN109661972A (en) | A kind of selection of high temperature resistant mushroom | |
| CN105660709B (en) | A kind of method for the accelerating agent of Chinese yew root growth and its for crossbreeding | |
| CN101914469A (en) | Bacillus cereus ectotrophic mycorrhiza helper bacteria and use thereof | |
| CN102150571B (en) | Production method for biological source theanine raw material | |
| CN106508362A (en) | Seedling-raising method for increasing seeding germination rate and seedling transplanting preservation rate of axillary choerospondias fruits |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20160509 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20170110 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20170116 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 6085889 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| LAPS | Cancellation because of no payment of annual fees |