CN108207487A - A kind of cultural method of agaric - Google Patents

A kind of cultural method of agaric Download PDF

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Publication number
CN108207487A
CN108207487A CN201810076097.2A CN201810076097A CN108207487A CN 108207487 A CN108207487 A CN 108207487A CN 201810076097 A CN201810076097 A CN 201810076097A CN 108207487 A CN108207487 A CN 108207487A
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China
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bacterium bag
bag
water
bacterium
bed
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CN201810076097.2A
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郑传勇
季占军
李日静
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Yunnan Qiang Feng Agricultural Science And Technology Co Ltd
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Yunnan Qiang Feng Agricultural Science And Technology Co Ltd
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Priority to CN201810076097.2A priority Critical patent/CN108207487A/en
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D3/00Calcareous fertilisers
    • C05D3/02Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products

Abstract

The invention discloses a kind of cultural methods of agaric, including following methods step, matrix is stirred, is packed, is sterilized, is inoculated with, bacterium germination management, does bed, vernalization, outdoor management, picking and drying, the agaric nutritive value of invention cultivation is high, and production cost is low, reduce living contaminants, the fungus mycelium speed of growth is fast, and the quality of agaric is greatly improved with yield.

Description

A kind of cultural method of agaric
Technical field
The present invention relates to agricultural plantation technology field, more specifically, being a kind of cultural method of agaric.
Background technology
Agaric (English:Agaric), mainly it is grown in China and Japan.Most of China is northeast agaric and Qinling Mountains wood Ear.Agaric not only can be wild but also can cultivate, and kind fructification is in ear, lobate or cup-shaped, thin, edge is wavy, wide 3-10 Centimetre, it is 2 millimeters or so thick, it is bonded in matrix with the short handle or narrow thin facies posterior hepatis of side life.Color and luster is dark brown, and soft texture is in Colloidal substance, thin and flexible, when moistening, is translucent, is shunk when dry and becomes brittle cutin approximation keratin.Delicious flavour, can Element can meat or fish, it is full of nutrition.Agaric is sweet in flavor, mild-natured, has many medicinal efficacies.Energy QI invigorating salubrity, has promoting blood circulation efficiency, and can prevent Control hypoferric anemia etc.;Can blood-nourishing preserve youthful looks, it is ruddy to make us skin, radiant, can dredge stomach, lubricates enteron aisle, while right Hypertensive patient also has certain help.Because agaric has a higher nutritive value, and wild agaric negligible amounts, therefore, people The agaric that work is cultivated is more and more, and cultivation agaric technology is also more and more skillful.
Invention content
In order to insufficient more than making up, the present invention provides a kind of cultural methods of agaric.
The scheme of the invention is:
1) by matrix spice, spice will be mixed thoroughly, ensure water content 65% or so;
2) it packs, the matrix artificial after stirring evenly is wanted to be compacted with hand in charging when feeding, and makes described in bacterium bag Elasticity is consistent up and down for matrix;
3) it sterilizes, it is available to frame up, shelve and sterilize using normal-pressure sterilization, high pressure sterilization mode, it treats warm in bacterium bag Degree up to keeping 12 hours or more after 100 DEG C again;It is taken the dish out of the pot while hot after sterilizing when bacterium bag temperature drops to 60 DEG C, bacterium bag feeding is connect Kind room is cooled down;
4) it is inoculated with, when material temperature in bacterium bag is down to below 30 DEG C, so that it may be inoculated with;
5) bacterium germination management will put culturing room's bacterium germination after sterilization after bacterium bag inoculation, Initial stage of culture, bacterium bag should be upright whole Put together,, can be opposite by bacterium bag bag bottom after mycelia is stretched into compost there are appropriately distance between bacterium bag, mouth outwardly, It is sleeping to put 2 rows, 4 rows are repeatedly folded up and down;Bacterium bag is put into " # " shape during bacterium germination, 9-11 layers high, one layer of the bacterium bag of Initial stage of culture four, per pile Between will be there are appropriately distance, as the raising of temperature can be changed to three one layer or two one layer;
6) make bed, selection of land gesture is flat, close to water source, draining is good, Around the house vacant lot, and requires to avoid air port;Bed with There are gutters between bed, and after bed is carried out, bed surface should pour a heavy water, then spray 500 times of thiophanate methyl solution disinfections;
7) vernalization, the bacterium bag being inoculated with were cultivated through 40~50 days, and mycelia can cover with bacterium bag;After mycelia is covered with, continue to train It supports 10~15 days, culturing room's shading, while reduces humidity, cultured bacterium bag, which can transport to, does a place, infects the bacterium of miscellaneous bacteria Bag will individually be put, and be placed on last opening, and when transport will handle with care, be carried out disinfection with 1% limewash to entire bacterium bag, then It is drained after pulling out, bacterium bag removes outer bag, then pricks hole, hole depth 0.5cm;Every bag of bacterium bag pricks 100 to 200 holes, the bottom It hole should more than 5cm from the ground;The bacterium bag of two beds is emitted on progress vernalization management in a bed;After discharge, modeling is covered Expect film, woven straw is covered on film, then into vernalization period management;Bacterium bag after trepanning enters vernalization management phase, this Phase does not water in bacterium bag, it should be noted that the humidity in bed, monitor during this bed surface relative air humidity should keep 85%~ 90%;After ear base is formed, into produce agaric period management, until ear bud grows to 1cm or so, start point bed and enter outdoor management;
8) outdoor management needs to be laid with one layer of mulch on the ground before dividing bed, and bacterium bag must handle with care, uprightly put, often put down Square rice puts 20 bags, bag interval 10cm, and spray water belt is spread on pavement, and water pump is controlled with autocontrol switch and sprayed water;
9) it picks, when the spore of auricle behind appearance white, is harvested when reaching medium well;
10) air dry, wants timely air dry after adopting, when air dry wants auricle upper, and ear base is under, by ear during the air dry of big Piece is torn, and into single sheet, air dry mesh, full communication between the higher and lower levels, air dry midway can not be stirred, disposably dry.
As preferred technical solution, the step 1) mesostroma includes mulberry branch, crops bagasse, peanut shell, huge Juncao, precipitated calcium carbonate, lime, titanium of obliging, neutral water.
As preferred technical solution, when bacterium bag feeds in the step 2) with indeformable, bag face without broken pleat, smooth be Standard, cultigen bacterium bag packaging is using charging, compacting, tying, and 1.5 kilograms of cultigen weight in wet base, the same day bacterium bag of dress will be on the day of Sterilizing.
As preferred technical solution, the bacterium bag of cooling is put into inoculating hood;75% wipes of alcohol of cultigen bottle outer wall It is also placed in inoculating hood after wiping disinfection, then with every cubic metre of 5 grams of potassium permanganate, 10 milliliters of formaldehyde are smoked 0.5~1 hour;Inoculation When light alcolhol burner, cultigen is broken into pieces with the tweezers of sterilizing, in the aseptic area for lighting alcolhol burner, makes the bottle of cultigen bottle Strain is equably sprinkling upon in bacterium bag and expects on surface, form a thin layer, every bottle of cultigen bottle kind can against the sack of bacterium bag by mouth 14-16 bags of inoculum bag.
As preferred technical solution, the step 5) middle culture early period is in 15 days, when cultivating early period, trains after inoculation The temperature for supporting room is maintained at 22 DEG C~25 DEG C;After mid-term is cultivated as inoculation 15 days, when cultivating mid-term, by the temperature liter of culturing room Height is to 26 DEG C or so;Late stage of culture is that culture is down to 20 DEG C~24 by 10 days of end, then the temperature of culturing room DEG C, temperature during bacterium germination in bacterium bag is 32 DEG C hereinafter, the humidity of culturing room is generally kept between 55%~65%, culture The close dark of indoor light;Culturing room will divulge information 20~30 minutes daily;Late stage of culture will increase ventilation time and number, protect It is fresh to hold culture room air.
As preferred technical solution, it is between pore size distribution mode uses Kong Yukong in every bag of bacterium bag in the step 7) The one of which of hole even distribution pattern on isosceles triangle arrangement mode or bacterium bag.
The bacterium bag of two beds is emitted in a bed as preferred technical solution, in the step 7) and carries out vernalization The discharge method of management:Every square metre can put 20 bags, stay the distance of 3cm between bag and bag, ground not spreading during discharge, row The drying of when putting such as ground need to spray bottom water;Ground it is moister can direct emission.
As preferred technical solution, the bacterium bag after the step 7) split shed enters vernalization management phase, monitors bed surface The method that relative air humidity should keep 85%~90%:See on plastic film whether there is water mist or droplet, if any being dripped under droplet, Then humidity is excessive, to increase degree of ventilation;Without water mist or droplet on plastic foil, it is that humidity is too small, reduces ventilation quantity, and need Spraying water in bed both sides increases bed surface humidity, determines the size of ventilation quantity according to humidity condition, bed surface during vernalization management phase If temperature will be controlled at 24 DEG C hereinafter, temperature is more than 24 DEG C, should divulge information makes temperature drop to less than 24 DEG C.
As preferred technical solution, after step 7) the middle ear base is formed, into produce agaric period management, the produce agaric phase Management method:Directly to feed water, when feedwater, removes woven straw, film, gives the lower film of water recession, covers the woven straw of wetting, Wetness in bed is kept, confluent should not be excessive, and prevents water from pouring into bag, should be according to humidity, degree of ventilation, natural epidemic disaster in booth in bed Degree, ear bud situation etc. consider decision, and bud is made to be maintained at good growth conditions, is aerated at dusk daily, until ear bud Grow to 1cm or so.
As preferred technical solution, described in the step 8) with water for limpid free of contamination river water or well water wherein One kind, the pH value of water is neutral, is sprayed water in 3 points to 7 points and 15 points to 19 points two periods.
By adopting the above-described technical solution, an a kind of agaric load kind method, 1) by matrix spice, spice will be mixed thoroughly, ensure Water content is 65% or so;
2) it packs, the matrix artificial after stirring evenly is wanted to be compacted with hand in charging when feeding, and makes described in bacterium bag Elasticity is consistent up and down for matrix;
3) it sterilizes, it is available to frame up, shelve and sterilize using normal-pressure sterilization, high pressure sterilization mode, it treats warm in bacterium bag Degree up to keeping 12 hours or more after 100 DEG C again;It is taken the dish out of the pot while hot after sterilizing when bacterium bag temperature drops to 60 DEG C, bacterium bag feeding is connect Kind room is cooled down;
4) it is inoculated with, when material temperature in bacterium bag is down to below 30 DEG C, so that it may be inoculated with;
5) bacterium germination management will put culturing room's bacterium germination after sterilization after bacterium bag inoculation, Initial stage of culture, bacterium bag should be upright whole Put together,, can be opposite by bacterium bag bag bottom after mycelia is stretched into compost there are appropriately distance between bacterium bag, mouth outwardly, It is sleeping to put 2 rows, 4 rows are repeatedly folded up and down;Bacterium bag is put into " # " shape during bacterium germination, 9-11 layers high, one layer of the bacterium bag of Initial stage of culture four, per pile Between will be there are appropriately distance, as the raising of temperature can be changed to three one layer or two one layer;
6) make bed, selection of land gesture is flat, close to water source, draining is good, Around the house vacant lot, and requires to avoid air port;Bed with There are gutters between bed, and after bed is carried out, bed surface should pour a heavy water, then spray 500 times of thiophanate methyl solution disinfections;
7) vernalization, the bacterium bag being inoculated with were cultivated through 40~50 days, and mycelia can cover with bacterium bag;After mycelia is covered with, continue to train It supports 10~15 days, culturing room's shading, while reduces humidity, cultured bacterium bag, which can transport to, does a place, infects the bacterium of miscellaneous bacteria Bag will individually be put, and be placed on last opening, and when transport will handle with care, be carried out disinfection with 1% limewash to entire bacterium bag, then It is drained after pulling out, bacterium bag removes outer bag, then pricks hole, hole depth 0.5cm;Every bag of bacterium bag pricks 100 to 200 holes, the bottom It hole should more than 5cm from the ground;The bacterium bag of two beds is emitted on progress vernalization management in a bed;After discharge, modeling is covered Expect film, woven straw is covered on film, then into vernalization period management;Bacterium bag after trepanning enters vernalization management phase, this Phase does not water in bacterium bag, it should be noted that the humidity in bed, monitor during this bed surface relative air humidity should keep 85%~ 90%;After ear base is formed, into produce agaric period management, until ear bud grows to 1cm or so, start point bed and enter outdoor management;
8) outdoor management needs to be laid with one layer of mulch on the ground before dividing bed, and bacterium bag must handle with care, uprightly put, often put down Square rice puts 20 bags, bag interval 10cm, and spray water belt is spread on pavement, and water pump is controlled with autocontrol switch and sprayed water;
9) it picks, when the spore of auricle behind appearance white, is harvested when reaching medium well;
10) air dry, wants timely air dry after adopting, when air dry wants auricle upper, and ear base is under, by ear during the air dry of big Piece is torn, and into single sheet, air dry mesh, full communication between the higher and lower levels, air dry midway can not be stirred, disposably dry.
The invention has the advantage that:
The present invention by the use of agricultural product crops bagasse, peanut shell and byproduct as fungus cultivation culture medium Fungus cultivation is carried out, because of stalk and the high nutrition containing higher suitable agaric growth in husk and byproduct of agricultural product. Under using culture medium of the present invention and cultural method collective effect of the present invention, cultivation gained agaric yield is higher, nutritive value It is higher;Further, the raw material as culture medium such as the stalk of agricultural product of the present invention and husk and byproduct, Production cost is greatly reduced, while the anti-miscellaneous bacteria of this method and premunition are strong, yield and the quality of agaric have obtained conspicuousness It improves.
Specific embodiment
In order to be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention, below In conjunction with specific embodiments, the present invention is further explained.
Embodiment one:
(1) matrix mixes, and takes mulberry branch, crops bagasse, peanut shell, Jujun grasses, precipitated calcium carbonate, lime, obliges Titanium, neutral water are mixed.
(2) technological process
Bed → vernalization → outdoor management → picking → air dry is done in spice → pack → sterilizing → inoculation → bacterium germination management →
1) spice is carried out after matrix is mixed.Spice will be mixed thoroughly, ensure water content 65% or so.
2) it packs.Want to be compacted with hand in charging during hand charging, it is desirable that upper and lower elasticity it is consistent (when bacterium bag feeds with Indeformable, bag face without broken pleat, it is smooth be standard).Original seed bacterium bag packaging using charging → compacting charge level → set it is bad → upper shield.It plants Bacterium bag packaging is cultivated using charging → compacting → tying.0.3 kilogram of original seed weight in wet base, 1.5 kilograms of cultigen weight in wet base.Same day dress Bacterium bag (bottle) will sterilize on the day of, it is impossible to stand overnight, in order to avoid generating miscellaneous bacteria, ferment, is rancid.If the same day cannot sterilize, Ying Fang To cooling, ventilation is stayed overnight.
3) it sterilizes.It is available to frame up, shelve and sterilize using normal-pressure sterilization, high pressure sterilization mode, it treats warm in bacterium bag Degree up to keeping 12 hours or more after 100 DEG C again.It is taken the dish out of the pot while hot after sterilizing when bacterium bag temperature drops to 60 DEG C, bacterium bag feeding is connect Kind room is cooled down.
4) it is inoculated with.When material temperature in bag is down to below 30 DEG C, so that it may be inoculated with.Inoculation will accomplish sterile working, bacterium bag Vaccine program is:The bacterium bag of cooling is put into inoculating hood;Cultigen bottle outer wall is also placed in after being sterilized with 75% alcohol wipe and connects In plants case, then with every cubic metre of 5 grams of potassium permanganate, 10 milliliters of formaldehyde are smoked 0.5 hour.Alcolhol burner is lighted during inoculation, with sterilizing Tweezers cultigen is broken into pieces, in the aseptic area for lighting alcolhol burner, make bottleneck that strain are equably sprinkling upon bag against sack On interior material surface, a thin layer is formed, such black fungus mycelium germination is fast, captures charge level, miscellaneous bacteria to be inhibited to infect.Every bottle Three-level kind connects 15 bags.
5) bacterium germination management.Bacterium bag connects the culturing room's bacterium germination that put after bacterium after sterilization, and Initial stage of culture, bag should be uprightly neat It puts, there are appropriately distances between bag, can be opposite by bag bottom after mycelia is stretched into compost, and mouth is outwardly, sleeping to put 2 rows, 4 rows are repeatedly folded up and down.Culture bag is put into " # " shape during bacterium bag bacterium germination, high ten layers or so, one layer of the bacterium bag of initial stage four often will between pile There are appropriately distance, as the raising of temperature can be changed to three one layer.
Early period is cultivated, that is, after being inoculated in 15 days, the temperature of culturing room is appropriate lower, is maintained at 22 DEG C DEG C, makes just inoculated Mycelia slowly restoration ecosystem, mycelia is sturdy vitality, can reduce living contaminants.Mid-term, that is, after being inoculated with 15 days, black fungus bacterial Silk growth is dominant, and temperature is increased to 26 DEG C or so, accelerates bacterium germination speed.In the later stage, when mycelia sends out completely soon, i.e. culture will In 10 days terminated, then temperature is down to 20 DEG C DEG C, mycelia grows healthy and strong at a lower temperature, nutrition decompose absorb it is abundant. The bacterium bag produce agaric turned out in this way is early, and differentiation is fast, and premunition is strong, and yield is high.Temperature during bacterium germination in bacterium bag must be controlled always System is at 32 DEG C hereinafter, subject to the more than temperature survey number second layer and lowest level.The humidity of culturing room be generally kept in 55% it Between.Black fungus does not need to light in the cultural hypha stage, and the window of culturing room will paste newspaper over, makes indoor light close to black Secretly.Culturing room will divulge information 20 minutes daily.Guarantee has enough oxygen to maintain the normal metabolism of black fungus mycelia.Afterwards Phase will more increase ventilation time and number, keep culture room air fresh.
6) bed is made.Selection of land gesture is flat, close to water source, draining is good, Around the house vacant lot, and requires to avoid air port.Soil property The plot for gluing weight is made away from 5cm, and the unlimited bed of wide 150cm length, there are gutters between bed and bed.After bed is carried out, bed surface should pour Then heavy water sprays 500 times of thiophanate methyl solution disinfections.
7) vernalization.The culture bag being inoculated with was cultivated through 40 days, and mycelia can cover with bacterium bag.Mycelia does not rush after covering with Ear is urged, should be cultivated for 10 days, makes the abundant material feeding of mycelia, gathers nutriment, improves anti-mildew disease resistance.At this moment, it cultivates Shading is wanted in room, while suitably reduces humidity, prevents ear bud from occurring and mycelia aging.Cultured bacterium bag can transport place to, sense The bacterium bag of dye miscellaneous bacteria will individually be put, and be placed on last opening.It to handle with care during transport.Entire bacterium bag is carried out with 1% limewash Disinfection, drains after then pulling out.(14cm × 55cm) bacterium bag removes outer bag, then pricks hole, hole depth 0.5cm;Every bag of bacterium bag is pricked 100 holes, the hole of the bottom should more than 5cm from the ground.It is arranged between Kong Yukong in isosceles triangle.Also it can be punched with puncher, Even distribution, this hole knockout auricle are mostly monolithic, and product quality is high.The bacterium bag of two beds is placed in a bed and is carried out Vernalization management.Every square metre can put 20 bags (14cm × 55cm bacterium bags can put 20 bags), and the distance of 3cm is stayed between bag and bag, discharge When ground not spreading, the drying of during discharge such as ground need to spray bottom water;Ground is moister directly to put.After discharge, Plastic film is covered, woven straw is covered on film, then into vernalization period management.Bacterium bag after opening enters vernalization management rank Section, this phase can not water on bag, it should be noted that the humidity in bed, bed surface relative air humidity should keep 85% during this.It crosses It is unfavorable for opening wound reparation in drying;Humidity is too big, and mycelia is easily pierced by from opening, influences ear base and is formed.Short-cut method It is:See on plastic film whether there is water mist or droplet, if any being dripped under droplet, then humidity is excessive, to increase degree of ventilation;On plastic foil It is that humidity is too small without water mist or droplet, reduces ventilation quantity, and needs increase bed surface humidity of spraying water in bed both sides.According to humidity Situation determines the size of ventilation quantity.Bed surface temperature will be controlled at 24 DEG C hereinafter, if temperature is more than 24 DEG C during this, Ying Tong Wind makes temperature drop to less than 24 DEG C.After ear base is formed, into produce agaric period management.The management main points in this period are:Directly to Water, when feedwater, remove woven straw, film, give the lower film of water recession, cover the woven straw of wetting, keep wetness in bed, Confluent should not be excessive, and prevents water from pouring into bag, should be comprehensive according to humidity, degree of ventilation, natural temperature, ear bud situation etc. in bed It closes and considers to determine.Bud is made to be maintained at good growth conditions, is aerated at dusk daily, until ear bud grows to 1cm or so, i.e., Ear bud will be higher by bacterium bag plane after doing, and start point bed and enter outdoor management.
8) outdoor management.It needs to be laid with one layer of mulch on the ground before dividing bed, bacterium bag must handle with care, uprightly put (14cm × 55cm bacterium bags will be cut open from centre), every square metre is put 20 bags, bag interval 10cm, in order to avoid agaric grows up, adhesion and influence are empty The circulation of gas.Spray water belt is spread on pavement, water pump is controlled with autocontrol switch and sprayed water.
Production black fungus water spray is a ring of most critical.To use limpid free of contamination river water or well water.During pH value is Property, it is sprayed water using special water spray facility, used water spray facility mainly has micro- spray nozzle, spray water belt etc..Water spraying time should Reasonable arrangement cannot change once fixed.If started from childhood, water sooner or later, then can not to the last become, it is impossible to Regardless of when think watering just water.It can take:It is 3 points to 7 points early, 15 points to 19 points at night of sprinkling method.Dry dry and wet Wet, alternation of wetting and drying, black fungus drought tolerance is strong, after ear bud and auricle drying contraction, under suitable damp condition, can restore raw Long development.When dry, mycelia growth accumulates nutrient;Auricle is grown during moistening, consumes nutrient, in entirely management period, should be slapped " preceding dry rear wet " is held, forms the management method of holding " dry dry and wet is wet, alternation of wetting and drying " after ear bud.
9) it picks.When the spore of auricle behind appearance white, to be harvested in time when reaching medium well.If treating, auricle extends Or it is picked again when scrolling up and will influence quality and yield.It is cut off, not kept with saw foam in ear Ji Chu with blade during harvesting The cleaning of auricle.It can not grasp and be backed out at auricle note root.
10) air dry.Want timely air dry after adopting, when air dry wants auricle upper, and ear base is under, by ear during the air dry of big Piece is torn, into single sheet.Air dry mesh, full communication between the higher and lower levels.Air dry midway can not be stirred, and disposably dry.
Embodiment two,
(1) matrix mixes, and takes mulberry branch, crops bagasse, peanut shell, Jujun grasses, precipitated calcium carbonate, lime, obliges Titanium, neutral water are mixed.
(2) technological process
Bed → vernalization → outdoor management → picking → air dry is done in spice → pack → sterilizing → inoculation → bacterium germination management →
1) spice is carried out after matrix is mixed.Spice will be mixed thoroughly, ensure water content 65% or so.
2) it packs.Want to be compacted with hand in charging during hand charging, it is desirable that upper and lower elasticity it is consistent (when bacterium bag feeds with Indeformable, bag face without broken pleat, it is smooth be standard).Original seed bacterium bag packaging using charging → compacting charge level → set it is bad → upper shield.It plants Bacterium bag packaging is cultivated using charging → compacting → tying.0.3 kilogram of original seed weight in wet base, 1.5 kilograms of cultigen weight in wet base.Same day dress Bacterium bag (bottle) will sterilize on the day of, it is impossible to stand overnight, in order to avoid generating miscellaneous bacteria, ferment, is rancid.If the same day cannot sterilize, Ying Fang To cooling, ventilation is stayed overnight.
3) it sterilizes.It is available to frame up, shelve and sterilize using normal-pressure sterilization, high pressure sterilization mode, it treats warm in bacterium bag Degree up to keeping 12 hours or more after 100 DEG C again.It is taken the dish out of the pot while hot after sterilizing when bacterium bag temperature drops to 60 DEG C, bacterium bag feeding is connect Kind room is cooled down.
4) it is inoculated with.When material temperature in bag is down to below 30 DEG C, so that it may be inoculated with.Inoculation will accomplish sterile working, bacterium bag Vaccine program is:The bacterium bag of cooling is put into inoculating hood;Cultigen bottle outer wall is also placed in after being sterilized with 75% alcohol wipe and connects In plants case, then with every cubic metre of 5 grams of potassium permanganate, 10 milliliters of formaldehyde are smoked 1 hour.Alcolhol burner is lighted during inoculation, with sterilizing Tweezers break cultigen into pieces, in the aseptic area for lighting alcolhol burner, bottleneck are made equably to be sprinkling upon strain in bag against sack Expect on surface, form a thin layer, such black fungus mycelium germination is fast, captures charge level, miscellaneous bacteria to be inhibited to infect.Every bottle three Grade kind can connect 16 bags.
5) bacterium germination management.Bacterium bag connects the culturing room's bacterium germination that put after bacterium after sterilization, and Initial stage of culture, bag should be uprightly neat It puts, there are appropriately distances between bag, can be opposite by bag bottom after mycelia is stretched into compost, and mouth is outwardly, sleeping to put 2 rows, 4 rows are repeatedly folded up and down.Culture bag is put into " # " shape during bacterium bag bacterium germination, high ten layers or so, one layer of the bacterium bag of initial stage four often will between pile There are appropriately distance, as the raising of temperature can be changed to two one layer.
Early period is cultivated, that is, after being inoculated in 15 days, the temperature of culturing room is appropriate lower, is maintained at 25 DEG C, makes just inoculated bacterium Silk slowly restoration ecosystem, mycelia is sturdy vitality, can reduce living contaminants.Mid-term, that is, after being inoculated with 15 days, black fungus mycelia Growth is dominant, and temperature is increased to 26 DEG C or so, accelerates bacterium germination speed.In the later stage, when mycelia sends out completely soon, i.e. culture will knot In 10 days of beam, then temperature is down to 24 DEG C, mycelia grows healthy and strong at a lower temperature, nutrition decompose absorb it is abundant.In this way The bacterium bag produce agaric turned out is early, and differentiation is fast, and premunition is strong, and yield is high.Temperature during bacterium germination in bacterium bag must control always 32 DEG C hereinafter, subject to the more than temperature survey number second layer and lowest level.The humidity of culturing room is generally kept between 65%.It is black Agaric does not need to light in the cultural hypha stage, and the window of culturing room will paste newspaper over, makes indoor light close to dark.Culture Room will divulge information 30 minutes daily.Guarantee has enough oxygen to maintain the normal metabolism of black fungus mycelia.Later stage more will Increase ventilation time and number, keep culture room air fresh.
6) bed is made.Selection of land gesture is flat, close to water source, draining is good, Around the house vacant lot, and requires to avoid air port.Soil property The plot for gluing weight is made away from 5cm, and the unlimited bed of wide 150cm length, there are gutters between bed and bed.After bed is carried out, bed surface should pour Then heavy water sprays 500 times of thiophanate methyl solution disinfections.
7) vernalization.The culture bag being inoculated with was cultivated through 50 days, and mycelia can cover with bacterium bag.Mycelia does not rush after covering with Ear is urged, should be cultivated for 15 days, makes the abundant material feeding of mycelia, gathers nutriment, improves anti-mildew disease resistance.At this moment, it cultivates Shading is wanted in room, while suitably reduces humidity, prevents ear bud from occurring and mycelia aging.Cultured bacterium bag can transport place to, sense The bacterium bag of dye miscellaneous bacteria will individually be put, and be placed on last opening.It to handle with care during transport.Entire bacterium bag is carried out with 1% limewash Disinfection, drains after then pulling out.(14cm × 55cm) bacterium bag removes outer bag, then pricks hole, hole depth 0.5cm;Every bag of bacterium bag is pricked 200 holes, the hole of the bottom should more than 5cm from the ground.It is arranged between Kong Yukong in isosceles triangle.Also it can be punched with puncher, Even distribution, this hole knockout auricle are mostly monolithic, and product quality is high.The bacterium bag of two beds is placed in a bed and is carried out Vernalization management.Every square metre can put 20 bags (14cm × 55cm bacterium bags can put 20 bags), and the distance of 3cm is stayed between bag and bag, discharge When ground not spreading, the drying of during discharge such as ground need to spray bottom water;Ground is moister directly to put.After discharge, Plastic film is covered, woven straw is covered on film, then into vernalization period management.Bacterium bag after opening enters vernalization management rank Section, this phase can not water on bag, it should be noted that the humidity in bed, bed surface relative air humidity should keep 90% during this.It crosses It is unfavorable for opening wound reparation in drying;Humidity is too big, and mycelia is easily pierced by from opening, influences ear base and is formed.Short-cut method It is:See on plastic film whether there is water mist or droplet, if any being dripped under droplet, then humidity is excessive, to increase degree of ventilation;On plastic foil It is that humidity is too small without water mist or droplet, reduces ventilation quantity, and needs increase bed surface humidity of spraying water in bed both sides.According to humidity Situation determines the size of ventilation quantity.Bed surface temperature will be controlled at 24 DEG C hereinafter, if temperature is more than 24 DEG C during this, Ying Tong Wind makes temperature drop to less than 24 DEG C.After ear base is formed, into produce agaric period management.The management main points in this period are:Directly to Water, when feedwater, remove woven straw, film, give the lower film of water recession, cover the woven straw of wetting, keep wetness in bed, Confluent should not be excessive, and prevents water from pouring into bag, should be comprehensive according to humidity, degree of ventilation, natural temperature, ear bud situation etc. in bed It closes and considers to determine.Bud is made to be maintained at good growth conditions, is aerated at dusk daily, until ear bud grows to 1cm or so, i.e., Ear bud will be higher by bacterium bag plane after doing, and start point bed and enter outdoor management.
8) outdoor management.It needs to be laid with one layer of mulch on the ground before dividing bed, bacterium bag must handle with care, uprightly put (14cm × 55cm bacterium bags will be cut open from centre), every square metre is put 20 bags, bag interval 10cm, in order to avoid agaric grows up, adhesion and influence are empty The circulation of gas.Spray water belt is spread on pavement, water pump is controlled with autocontrol switch and sprayed water.
Production black fungus water spray is a ring of most critical.To use limpid free of contamination river water or well water.During pH value is Property, it is sprayed water using special water spray facility, used water spray facility mainly has micro- spray nozzle, spray water belt etc..Water spraying time should Reasonable arrangement cannot change once fixed.If started from childhood, water sooner or later, then can not to the last become, it is impossible to Regardless of when think watering just water.It can take:It is 3 points to 7 points early, 15 points to 19 points at night of sprinkling method.Dry dry and wet Wet, alternation of wetting and drying, black fungus drought tolerance is strong, after ear bud and auricle drying contraction, under suitable damp condition, can restore raw Long development.When dry, mycelia growth accumulates nutrient;Auricle is grown during moistening, consumes nutrient, in entirely management period, should be slapped " preceding dry rear wet " is held, forms the management method of holding " dry dry and wet is wet, alternation of wetting and drying " after ear bud.
9) it picks.When the spore of auricle behind appearance white, to be harvested in time when reaching medium well.If treating, auricle extends Or it is picked again when scrolling up and will influence quality and yield.It is cut off, not kept with saw foam in ear Ji Chu with blade during harvesting The cleaning of auricle.It can not grasp and be backed out at auricle note root.
10) air dry.Want timely air dry after adopting, when air dry wants auricle upper, and ear base is under, by ear during the air dry of big Piece is torn, into single sheet.Air dry mesh, full communication between the higher and lower levels.Air dry midway can not be stirred, and disposably dry.
Embodiment three,
(1) matrix mixes, and takes mulberry branch, crops bagasse, peanut shell, Jujun grasses, precipitated calcium carbonate, lime, obliges Titanium, neutral water are mixed.
(2) technological process
Bed → vernalization → outdoor management → picking → air dry is done in spice → pack → sterilizing → inoculation → bacterium germination management →
1) spice is carried out after matrix is mixed.Spice will be mixed thoroughly, ensure water content 65% or so.
2) it packs.Want to be compacted with hand in charging during hand charging, it is desirable that upper and lower elasticity it is consistent (when bacterium bag feeds with Indeformable, bag face without broken pleat, it is smooth be standard).Original seed bacterium bag packaging using charging → compacting charge level → set it is bad → upper shield.It plants Bacterium bag packaging is cultivated using charging → compacting → tying.0.3 kilogram of original seed weight in wet base, 1.5 kilograms of cultigen weight in wet base.Same day dress Bacterium bag (bottle) will sterilize on the day of, it is impossible to stand overnight, in order to avoid generating miscellaneous bacteria, ferment, is rancid.If the same day cannot sterilize, Ying Fang To cooling, ventilation is stayed overnight.
3) it sterilizes.It is available to frame up, shelve and sterilize using normal-pressure sterilization, high pressure sterilization mode, it treats warm in bacterium bag Degree up to keeping 12 hours or more after 100 DEG C again.It is taken the dish out of the pot while hot after sterilizing when bacterium bag temperature drops to 60 DEG C, bacterium bag feeding is connect Kind room is cooled down.
4) it is inoculated with.When material temperature in bag is down to below 30 DEG C, so that it may be inoculated with.Inoculation will accomplish sterile working, bacterium bag Vaccine program is:The bacterium bag of cooling is put into inoculating hood;Cultigen bottle outer wall is also placed in after being sterilized with 75% alcohol wipe and connects In plants case, then with every cubic metre of 5 grams of potassium permanganate, 10 milliliters of formaldehyde are smoked 0.8 hour.Alcolhol burner is lighted during inoculation, with sterilizing Tweezers cultigen is broken into pieces, in the aseptic area for lighting alcolhol burner, make bottleneck that strain are equably sprinkling upon bag against sack On interior material surface, a thin layer is formed, such black fungus mycelium germination is fast, captures charge level, miscellaneous bacteria to be inhibited to infect.Every bottle Three-level kind can connect 14 bags.
5) bacterium germination management.Bacterium bag connects the culturing room's bacterium germination that put after bacterium after sterilization, and Initial stage of culture, bag should be uprightly neat It puts, there are appropriately distances between bag, can be opposite by bag bottom after mycelia is stretched into compost, and mouth is outwardly, sleeping to put 2 rows, 4 rows are repeatedly folded up and down.Culture bag is put into " # " shape during bacterium bag bacterium germination, high ten layers or so, one layer of the bacterium bag of initial stage four often will between pile There are appropriately distance, as the raising of temperature can be changed to three one layer.
Early period is cultivated, that is, after being inoculated in 15 days, the temperature of culturing room is appropriate lower, is maintained at 23 DEG C, makes just inoculated bacterium Silk slowly restoration ecosystem, mycelia is sturdy vitality, can reduce living contaminants.Mid-term, that is, after being inoculated with 15 days, black fungus mycelia Growth is dominant, and temperature is increased to 26 DEG C or so, accelerates bacterium germination speed.In the later stage, when mycelia sends out completely soon, i.e. culture will knot In 10 days of beam, then temperature is down to 22 DEG C, mycelia grows healthy and strong at a lower temperature, nutrition decompose absorb it is abundant.In this way The bacterium bag produce agaric turned out is early, and differentiation is fast, and premunition is strong, and yield is high.Temperature during bacterium germination in bacterium bag must control always 32 DEG C hereinafter, subject to the more than temperature survey number second layer and lowest level.The humidity of culturing room is generally kept between 60%.It is black Agaric does not need to light in the cultural hypha stage, and the window of culturing room will paste newspaper over, makes indoor light close to dark.Culture Room will divulge information 25 minutes daily.Guarantee has enough oxygen to maintain the normal metabolism of black fungus mycelia.Later stage more will Increase ventilation time and number, keep culture room air fresh.
6) bed is made.Selection of land gesture is flat, close to water source, draining is good, Around the house vacant lot, and requires to avoid air port.Soil property The plot for gluing weight is made away from 5cm, and the unlimited bed of wide 150cm length, there are gutters between bed and bed.After bed is carried out, bed surface should pour Then heavy water sprays 500 times of thiophanate methyl solution disinfections.
7) vernalization.The culture bag being inoculated with was cultivated through 45 days, and mycelia can cover with bacterium bag.Mycelia does not rush after covering with Ear is urged, should be cultivated for 12 days, makes the abundant material feeding of mycelia, gathers nutriment, improves anti-mildew disease resistance.At this moment, it cultivates Shading is wanted in room, while suitably reduces humidity, prevents ear bud from occurring and mycelia aging.Cultured bacterium bag can transport place to, sense The bacterium bag of dye miscellaneous bacteria will individually be put, and be placed on last opening.It to handle with care during transport.Entire bacterium bag is carried out with 1% limewash Disinfection, drains after then pulling out.(14cm × 55cm) bacterium bag removes outer bag, then pricks hole, hole depth 0.5cm;Every bag of bacterium bag is pricked 150 holes, the hole of the bottom should more than 5cm from the ground.It is arranged between Kong Yukong in isosceles triangle.Also it can be punched with puncher, Even distribution, this hole knockout auricle are mostly monolithic, and product quality is high.The bacterium bag of two beds is placed in a bed and is carried out Vernalization management.Every square metre can put 20 bags (14cm × 55cm bacterium bags can put 20 bags), and the distance of 3cm is stayed between bag and bag, discharge When ground not spreading, the drying of during discharge such as ground need to spray bottom water;Ground is moister directly to put.After discharge, Plastic film is covered, woven straw is covered on film, then into vernalization period management.Bacterium bag after opening enters vernalization management rank Section, this phase can not water on bag, it should be noted that the humidity in bed, bed surface relative air humidity should keep 87% during this.It crosses It is unfavorable for opening wound reparation in drying;Humidity is too big, and mycelia is easily pierced by from opening, influences ear base and is formed.Short-cut method It is:See on plastic film whether there is water mist or droplet, if any being dripped under droplet, then humidity is excessive, to increase degree of ventilation;On plastic foil It is that humidity is too small without water mist or droplet, reduces ventilation quantity, and needs increase bed surface humidity of spraying water in bed both sides.According to humidity Situation determines the size of ventilation quantity.Bed surface temperature will be controlled at 24 DEG C hereinafter, if temperature is more than 24 DEG C during this, Ying Tong Wind makes temperature drop to less than 24 DEG C.After ear base is formed, into produce agaric period management.The management main points in this period are:Directly to Water, when feedwater, remove woven straw, film, give the lower film of water recession, cover the woven straw of wetting, keep wetness in bed, Confluent should not be excessive, and prevents water from pouring into bag, should be comprehensive according to humidity, degree of ventilation, natural temperature, ear bud situation etc. in bed It closes and considers to determine.Bud is made to be maintained at good growth conditions, is aerated at dusk daily, until ear bud grows to 1cm or so, i.e., Ear bud will be higher by bacterium bag plane after doing, and start point bed and enter outdoor management.
8) outdoor management.It needs to be laid with one layer of mulch on the ground before dividing bed, bacterium bag must handle with care, uprightly put (14cm × 55cm bacterium bags will be cut open from centre), every square metre is put 20 bags, bag interval 10cm, in order to avoid agaric grows up, adhesion and influence are empty The circulation of gas.Spray water belt is spread on pavement, water pump is controlled with autocontrol switch and sprayed water.
Production black fungus water spray is a ring of most critical.To use limpid free of contamination river water or well water.During pH value is Property, it is sprayed water using special water spray facility, used water spray facility mainly has micro- spray nozzle, spray water belt etc..Water spraying time should Reasonable arrangement cannot change once fixed.If started from childhood, water sooner or later, then can not to the last become, it is impossible to Regardless of when think watering just water.It can take:It is 3 points to 7 points early, 15 points to 19 points at night of sprinkling method.Dry dry and wet Wet, alternation of wetting and drying, black fungus drought tolerance is strong, after ear bud and auricle drying contraction, under suitable damp condition, can restore raw Long development.When dry, mycelia growth accumulates nutrient;Auricle is grown during moistening, consumes nutrient, in entirely management period, should be slapped " preceding dry rear wet " is held, forms the management method of holding " dry dry and wet is wet, alternation of wetting and drying " after ear bud.
9) it picks.When the spore of auricle behind appearance white, to be harvested in time when reaching medium well.If treating, auricle extends Or it is picked again when scrolling up and will influence quality and yield.It is cut off, not kept with saw foam in ear Ji Chu with blade during harvesting The cleaning of auricle.It can not grasp and be backed out at auricle note root.
10) air dry.Want timely air dry after adopting, when air dry wants auricle upper, and ear base is under, by ear during the air dry of big Piece is torn, into single sheet.Air dry mesh, full communication between the higher and lower levels.Air dry midway can not be stirred, and disposably dry.
Basic principle, main feature and the advantages of the present invention of the present invention has been shown and described above.The technology of the industry Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this The principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these Changes and improvements all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by appended claims And its equivalent circle.

Claims (10)

1. a kind of cultural method of agaric, which is characterized in that including following following methods step:
1) by matrix spice, spice will be mixed thoroughly, ensure water content 65% or so;
2) it packs, the matrix artificial after stirring evenly is wanted to be compacted with hand in charging when feeding, and makes the matrix in bacterium bag Upper and lower elasticity is consistent;
3) it sterilizes, it is available to frame up, shelve and sterilize using normal-pressure sterilization, high pressure sterilization mode, treat bacterium bag Nei Wenduda It is kept again after 100 DEG C 12 hours or more;Taken the dish out of the pot while hot after sterilizing when bacterium bag temperature drops to 60 DEG C, by bacterium bag be sent into transfer room into Row cooling;
4) it is inoculated with, when material temperature in bacterium bag is down to below 30 DEG C, so that it may be inoculated with;
5) bacterium germination management, will put culturing room's bacterium germination after sterilization, Initial stage of culture after bacterium bag inoculation, bacterium bag uprightly should neatly be put It puts, there are appropriately distances between bacterium bag, can be opposite by bacterium bag bag bottom after mycelia is stretched into compost, and mouth is outwardly, sleeping to put 2 Row repeatedly folds 4 rows up and down;Bacterium bag is put into " # " shape during bacterium germination, 9-11 layers high, one layer of the bacterium bag of Initial stage of culture four will often be stayed between pile There is appropriately distance, as the raising of temperature can be changed to three one layer or two one layer;
6) make bed, selection of land gesture is flat, close to water source, draining is good, Around the house vacant lot, and requires to avoid air port;Bed with bed it Between there are gutters, after bed is carried out, bed surface should pour a heavy water, then spray 500 times of thiophanate methyl solution disinfections;
7) vernalization, the bacterium bag being inoculated with were cultivated through 40~50 days, and mycelia can cover with bacterium bag;After mycelia is covered with, continue culture 10 ~15 days, culturing room's shading, while humidity is reduced, cultured bacterium bag, which can transport to, does a place, and the bacterium bag for infecting miscellaneous bacteria will It individually puts, is placed on last opening, when transport will handle with care, be carried out disinfection with 1% limewash to entire bacterium bag, after then pulling out It drains, bacterium bag removes outer bag, then pricks hole, hole depth 0.5cm;Every bag of bacterium bag pricks 100 to 200 holes, and the hole of the bottom should be from More than ground 5cm;The bacterium bag of two beds is emitted on progress vernalization management in a bed;After discharge, plastic film is covered, Woven straw is covered on film, then into vernalization period management;Bacterium bag after trepanning enters vernalization management phase, this phase is not to bacterium bag Upper watering, it should be noted that the humidity in bed, monitoring bed surface relative air humidity should keep 85%~90% during this;Ear base is formed Afterwards, into produce agaric period management, until ear bud grows to 1cm or so, start point bed and enter outdoor management;
8) outdoor management needs to be laid with one layer of mulch on the ground before dividing bed, and bacterium bag must handle with care, uprightly put, every square metre 20 bags, bag interval 10cm are put, spray water belt is spread on pavement, water pump is controlled with autocontrol switch and sprayed water;
9) it picks, when the spore of auricle behind appearance white, is harvested when reaching medium well;
10) air dry, wants timely air dry after adopting, when air dry wants auricle upper, and for ear base under, when air dry of big tears auricle It opens, into single sheet, air dry mesh, full communication between the higher and lower levels, air dry midway can not be stirred, disposably dry.
2. a kind of cultural method of agaric as described in claim 1, it is characterised in that:Step 1) the mesostroma includes ramulus mori Item, crops bagasse, peanut shell, Jujun grasses, precipitated calcium carbonate, lime, titanium of obliging, neutral water.
3. a kind of cultural method of agaric as described in claim 1, it is characterised in that:In the step 2) bacterium bag feed when with Indeformable, bag face is without broken pleat, smooth for standard, and cultigen bacterium bag packaging is successively using charging, compacting, tying method, cultigen 1.5 kilograms of weight in wet base, the same day bacterium bag of dress will sterilize on the day of.
A kind of 4. cultural method of agaric as described in claim 1, which is characterized in that the method being inoculated in the step 4) For:The bacterium bag of cooling is put into inoculating hood;Cultigen bottle outer wall is also placed in after being sterilized with 75% alcohol wipe in inoculating hood, so Afterwards with every cubic metre of 5 grams of potassium permanganate, 10 milliliters of formaldehyde are smoked 0.5~1 hour;Alcolhol burner is lighted during inoculation, with the tweezers of sterilizing Cultigen is broken into pieces, in the aseptic area for lighting alcolhol burner, makes the bottleneck of cultigen bottle against the sack of bacterium bag, strain is uniform Ground is sprinkling upon in bacterium bag on material surface, forms a thin layer, every bottle of 14-16 bags of cultigen bottle kind inoculum bag.
5. a kind of cultural method of agaric as described in claim 1, it is characterised in that:Culture early period is connects in the step 5) After kind in 15 days, when cultivating early period, the temperature of culturing room is maintained at 22 DEG C~25 DEG C;After mid-term is cultivated as inoculation 15 days, culture During mid-term, the temperature of culturing room is increased to 26 DEG C or so;Late stage of culture be culture by 10 days of end, then culturing room Temperature is down to 20 DEG C~24 DEG C, and the temperature during bacterium germination in bacterium bag is for 32 DEG C hereinafter, the humidity of culturing room is generally kept in Between 55%~65%, the close dark of culture indoor light;Culturing room will divulge information 20~30 minutes daily;Late stage of culture will increase Add ventilation time and number, keep culture room air fresh.
6. a kind of cultural method of agaric as described in claim 1, it is characterised in that:It is pricked in every bag of bacterium bag in the step 7) Pore size distribution mode is using the one of which between hole and oral pore being in isosceles triangle arrangement mode or the hole even distribution pattern in bacterium bag.
7. a kind of cultural method of agaric as described in claim 1, which is characterized in that by the bacterium of two beds in the step 7) Bag is emitted on the discharge method that vernalization management is carried out in a bed:Every square metre can put 20 bags, stayed between bag and bag 3cm away from Ground not spreading during from, discharge, during discharge such as ground drying need to spray bottom water;Ground it is moister can direct emission.
A kind of 8. cultural method of agaric as described in claim 1, which is characterized in that the bacterium bag after the step 7) split shed Into vernalization management phase, the method that bed surface relative air humidity should keep 85%~90% is monitored:See on plastic film whether there is Water mist or droplet, if any being dripped under droplet, then humidity is excessive, to increase degree of ventilation;It is humidity mistake without water mist or droplet on plastic foil It is small, ventilation quantity is reduced, and need increase bed surface humidity of spraying water in bed both sides, the big of ventilation quantity is determined according to humidity condition Small, if bed surface temperature will be controlled at 24 DEG C hereinafter, temperature is more than 24 DEG C during vernalization management phase, should divulge information makes temperature drop to 24 Below DEG C.
9. a kind of cultural method of agaric as described in claim 1, which is characterized in that after step 7) the middle ear base is formed, Into produce agaric period management, the produce agaric period management method:It directly feeds water, when feedwater removes woven straw, film, gives water recession Lower film covers the woven straw of wetting, keeps wetness in bed, confluent should not be excessive, and prevents water from pouring into bag, should basis Humidity, degree of ventilation, natural temperature, ear bud situation etc. consider decision in bed, bud are made to be maintained at good growth conditions, daily It is aerated at dusk, until ear bud grows to 1cm or so.
10. a kind of cultural method of agaric as described in claim 1, it is characterised in that:It is with water described in the step 8) Limpid free of contamination river water or well water one of which, the pH value of water is neutral, in 3 points to 7 points and 15 points to 19 points two times Duan Jinhang sprays water.
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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109122051A (en) * 2018-08-31 2019-01-04 景洪宏臻农业科技有限公司 A kind of cultural method of Boletus aereus solid spawn
CN109511463A (en) * 2019-01-11 2019-03-26 昆明旭日丰华农业科技有限公司 A kind of novel breeding method of cloud ear
CN111919661A (en) * 2020-08-12 2020-11-13 南京康之春生物科技有限公司 Auricularia fuscosuccinea cultivation method
CN112352788A (en) * 2020-10-14 2021-02-12 王峰 Agent for preventing and treating mixed fungi and mold on fungus stick
CN115918438A (en) * 2022-12-16 2023-04-07 陕西理工大学 Breeding and cultivating method of new variety of black fungus

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MBAZUS: "黑木耳栽培技术", 《百度文库》 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109122051A (en) * 2018-08-31 2019-01-04 景洪宏臻农业科技有限公司 A kind of cultural method of Boletus aereus solid spawn
CN109122051B (en) * 2018-08-31 2021-06-15 景洪宏臻农业科技有限公司 Culture method of boletus aereus solid strain
CN109511463A (en) * 2019-01-11 2019-03-26 昆明旭日丰华农业科技有限公司 A kind of novel breeding method of cloud ear
CN111919661A (en) * 2020-08-12 2020-11-13 南京康之春生物科技有限公司 Auricularia fuscosuccinea cultivation method
CN112352788A (en) * 2020-10-14 2021-02-12 王峰 Agent for preventing and treating mixed fungi and mold on fungus stick
CN115918438A (en) * 2022-12-16 2023-04-07 陕西理工大学 Breeding and cultivating method of new variety of black fungus

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Application publication date: 20180629