CN103571788B - A kind of thecasporous Collection and preservation method of Cordyceps militaris (L.) Link. - Google Patents
A kind of thecasporous Collection and preservation method of Cordyceps militaris (L.) Link. Download PDFInfo
- Publication number
- CN103571788B CN103571788B CN201210282858.2A CN201210282858A CN103571788B CN 103571788 B CN103571788 B CN 103571788B CN 201210282858 A CN201210282858 A CN 201210282858A CN 103571788 B CN103571788 B CN 103571788B
- Authority
- CN
- China
- Prior art keywords
- sand
- collected
- link
- thecasporous
- cordyceps militaris
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a kind of thecasporous Collection and preservation method of Cordyceps militaris (L.) Link., collection method comprises the steps: sand to be put into many spores separated and collected container that can seal, sterilizing, drying; Select Cordyceps militaris (L.) Link. ascoma fully-developed sporophore, be placed with in many spores separated and collected container of sand; Many spores separated and collected of vibrating container, the thecasporous sand of maturation collected the thecaspore containing the maturation of launching out and do not launch out.The sand collected is placed in dry sterile chamber seal, room temperature lucifuge or refrigerator cold-storage are preserved.Collection method provided by the invention is simple to operate, improve thecasporous collection rate, can retain the inherited character of Cordyceps militaris (L.) Link. original parent after the thecaspore activation of preservation, and for screening degeneration-resistant new variety.
Description
Technical field
The invention belongs to cordyceps cultivation technology field, particularly, relate to a kind of thecasporous Collection and preservation method of Cordyceps militaris (L.) Link..
Background technology
Cordyceps militaris (L.) Link. Cordycepsmilitaris(L.exFr) Link, have another name called Cordyceps militaris (L.) Link., Cordyceps militaris, colonize on the insect pupal cells such as lepidopteran, Coleoptera, Diptera.Growth, in the region, Plain of low altitude area, is mainly distributed in the ground such as northeast, North China, northwest.Modern study shows, Cordyceps militaris (L.) Link. contains the chemical compositions such as cordycepin (3'-Deoxyadenosine), cordycepic acid (PEARLITOL 25C), adenosine, Cordyceps polysaccharide, ergosterol, superoxide-dismutase (SOD), selenium (Se).There is effects such as improving immune function of human body, anticancer, antibacterial, antifatigue, anti-ageing, anticonvulsion, hypoxia tolerance, calmness, establishing-Yang are reinforced the kidney.Also there is the effects such as fat-reducing, weight reducing, beauty treatment simultaneously.
In recent years, a lot of research all shows, tame Cordyceps militaris (L.) Link. chemical composition and pharmacological action similar to Cordyceps sinensis, but price is well below Cordyceps sinensis, and therefore, the Application and Development of Cordyceps militaris (L.) Link. has great potential market.Tissue Culture of Cordyceps militaris will obtain sporophore, and key is that this cordyceps militaris link bacterial strain will have the ability forming sporophore, and the genetic background that different strains possesses is different, so the ability that they possess the sporophore of formation is variant.But, in actual production, even advantage cordyceps militaris link bacterial strain, along with bacterial classification go down to posterity, the increase of number of times of transferring or the prolongation of preservation time, microorganism resource is very easily degenerated.
Spore separation method, is sprout into mycelia with the sexual spore of edible mushrooms or asexual spore, is trained the method for bacterial classification.This bacterial classification vitality is comparatively strong, but variant between spore individuality, and natural differentiation phenomenon is comparatively serious, and variation is large, need could application in production through fruiting experiment.Current spore separation method mainly contains: (one) single spore separation method: be each or often prop up test tube and only get a sporidium, allows it sprout into mycelium to obtain the method for pure strain.Single spore separation produces upper less employing, and technical sophistication, general employing many spores partition method.(2) many spores partition method be many spore inoculatings on same substratum, allow their are sprouted, pangamy to be to obtain a kind of method of edible mushrooms pure strain.Concrete operation method, has following several: 1. kind of mushroom spore launches method: eight or nine points that selection adaptation is strong ripe kind mushrooms, stem aseptic water washing number all over after blot surface-moisture with sterilized gauze or absorbent cotton, filter paper again.In inoculation tank or sterilisable chamber, the lamella of kind of mushroom is hung upside down below glass funnel with iron wire down, and funnel covers on culture dish; Upper end aperture clogs with cotton.Culture dish is placed on one and is covered with on the enamel tray of gauze, and leave standstill 12 ~ 20 hours, the spore on lamella will be scattered in culture dish.Form the Powdered spore print of one deck.Pick outside the agar of a small amount of spore in test tube or streak inoculation on culture dish with inoculating needle.Treat spore germination, when generating bacterium colony, select spore germination early, bacterium colony that growing way is good carries out Tube propagation.Also spore collected by available spore collector.By said procedure, raise bell glass gently, kind of a mushroom handle is inserted on the wire frame of spore collector down, is placed on culture dish centre.Build lens immediately, with gauze, the clock palm is around stoppered.And on gauze a little mercuric chloride or sterilized water.Move into about 20 DEG C thermostat containers to cultivate.2. streak method on pleat: select ripe kind mushroom, directly insert between gusset with inoculating needle, smears the spore got gusset surface sporophore and not yet launch gently, then streak inoculation on substratum.3. hook hangs method: several lamella or a fritter auricle (black fungus, Auricularia polytricha (Mout) Sacc., Tremella) of getting ripe cap, hang on the top of the substratum in triangular flask with aseptic Stainless Steel Wire (or other suspension materials such as iron wire, cotton thread), do not make to touch substratum or surrounding bottle wall.Cultivate under putting optimal temperature, transfer.4. attach method: the lamella of maturation or auricle are got a fritter, is attached on the test tube wall directly over pipe arrangement slant medium with the nutrient agar dissolved or gum arabic, paste etc.Through the cultivation of 6 ~ 12 hours, treat that spore drops on inclined-plane, spore is transplanted in new test tube together with part nutrient agar cultivates immediately.Mother's kind that spore separation obtains, to purificate and rejuvenate further, when mother plants field planting about one week, when mycelia is covered with inclined-plane, select mycelia healthy and strong, grow vigorous without aging, plant test tube without the mother infecting assorted mattress, and then tube expands.Mother's kind that spore separation obtains, must pass through fruiting experiment, after being accredited as good quality strain, just can supply production and application.There are two deficiencies in aforesaid method: the first, complicated operation.The second, can only collect ripe by the spore launched out, and but can not collected by the ripe spore launched out of maturation.
Summary of the invention
The object of this invention is to provide a kind of thecasporous Collection and preservation method of Cordyceps militaris (L.) Link., to overcome above-mentioned deficiency.
In order to realize the object of the invention, the thecasporous collection method of a kind of Cordyceps militaris (L.) Link. of the present invention, it comprises the steps:
1) sand is put into many spores separated and collected container that can seal, sterilizing, drying;
2) Cordyceps militaris (L.) Link. ascoma fully-developed sporophore puts into many spores separated and collected container of step 1);
3) many spores separated and collected of vibrating container, the thecasporous sand of maturation collected the thecaspore containing the maturation of launching out and do not launch out.
Wherein, described step 1) sand is neutral dry sand, and it obtains by the following method:
A) river sand is crossed 60 mesh sieves, discard macrobead and impurity;
B) sieve with 80 orders again, discard fine sand, collect unsifted sand;
C) irony in sand is removed;
D) with after the salt acid soak sand 24h of 10%-20%, acid of desalting, washes bubble with water to neutral, dries sand.
Further, the irony in magnet removing sand can be used in step c).
Many spores separated and collected container that the present invention uses, the sealable Glass Containers that can have any shape or metal vessel.
Wherein, the laying method that many spores separated and collected container that can seal put into by the sand described in step 1) be by etc. after the sand of quality mixes with distilled water, 1:3-10 is placed in many spores separated and collected container by volume.
Wherein, the sterilizing described in step 1) is 121 DEG C, moist heat sterilization 30min.
Wherein, the drying described in step 1) is 80 DEG C of dry 24-48h.
In the step of above-mentioned sterilizing and drying, many spores separated and collected container all strictly seals and carries out sterilizing and drying.
Wherein, step 2) the abundant sporophore head of the preferred 1-2cm ascoma of Cordyceps militaris (L.) Link. ascoma fully-developed sporophore, put into many spores separated and collected container of step 1), room temperature places 10-30 days, to sporophore complete drying.
Wherein, step 2) described in sporophore put into many spores separated and collected container, the sporophore number put into and the number mass ratio of container sand are 1: 2.5-5g.
Step 2 in Cordyceps militaris (L.) Link. thecaspore collection method provided by the invention) in, room temperature places 10-30 days, until sporophore complete drying.In the meantime, the ascoma that a part is ripe can launch thecaspore on fine sand by nature, have the ascoma of Partial mature not yet to launch thecaspore just dry, some not full ripe ascoma does not enter the period of launching, and thecaspore is still retained in dry ascoma.
Wherein, the vibration described in step 3) is with 80-200r/min speed oscillation 24-48h by many spores separated and collected container.
The thecasporous collection method of a kind of Cordyceps militaris (L.) Link. provided by the invention also comprises takes out the sporophore of drying.
The present invention also provides a kind of Cordyceps militaris (L.) Link. thecasporous store method, and the thecaspore containing the maturation of launching out obtained by aforesaid method and the thecasporous sand of maturation do not launched out are placed in dry sterile chamber sealing preservation.
Further, the present invention preserves the thecasporous storage conditions of Cordyceps militaris (L.) Link. is room temperature lucifuge or 0-6 DEG C of refrigeration.
The invention provides the application of Cordyceps militaris (L.) Link. thecaspore collection method in Cordyceps militaris (L.) Link. breeding.
The present invention utilizes the rubbing effect of fine sand, when the ascoma of the maturation of drying and immature ascoma are subject to the rubbing effect of fine sand thus broken and release thecaspore wherein, falls into sand carrier.By collecting the sand of macroscopic, thus collect the sightless Cordyceps militaris (L.) Link. thecaspore of naked eyes, therefore present method can not only collect the thecaspore of ejection, can also collect the thecaspore do not ejected, thecasporous collection rate reaches 100%, improves thecasporous collection effciency.And the thecaspore adopting catapult technique to collect in prior art is the parton cystospore launched out after ascoma maturation, thecaspore quantity is less than 1% of thecaspore total amounts all in sporophore ascoma.The sporophore of drying is taken out by the present invention further, by thecaspore activation or save backup, finally achieves the object of the thecasporous separation of Cordyceps militaris (L.) Link., collection and preservation.Cordyceps militaris (L.) Link. thecaspore collection method provided by the invention is simple to operate, use manpower and material resources sparingly, improve thecasporous collection rate simultaneously, the Cordyceps militaris (L.) Link. thecaspore store method provided effectively can keep thecasporous biological activity in 2-4, maintain the inherited character of original parent, for seed selection novel bacterial provides effective ways, be with a wide range of applications.
Embodiment
Following examples further illustrate content of the present invention, but should not be construed as limitation of the present invention.Without departing from the spirit and substance of the case in the present invention, the amendment do the inventive method, step or condition or replacement, all belong to scope of the present invention.
If do not specialize, the conventional means that technique means used in embodiment is well known to those skilled in the art.
The cultivation of embodiment 1 Cordyccps-militaris-(L.)-link. Sporophore
1, bacterial classification: the strain number adopting autonomous screening to obtain is HX-64 cordyceps militaris link bacterial strain (purchased from Beijing City Agriculture and Forestry Institute Bio-Centers).
2, liquid shaking bottle is with substratum (1L): peeled potatoes 300g(liquor), glucose 10g, peptone 2g, potassium primary phosphate 0.5g, magnesium sulfate 1g, water 1000ml, pH7.2.
3, sporophore produces nutritive medium: the ratio of soya bean 2%, glucose 1%, milk powder 5%, potassium primary phosphate 0.5%, magnesium sulfate 0.05%, VB11 0mg/1000ml is made into nutritive medium, and pH is 5.6 ~ 7.2 in adjustment.
4, PP is adopted to expect the cultivation box (go to the bottom diameter 8cm for high 10cm, upper base diameter 12cm) made.Every box rice 40g and sporophore produce nutritive medium 56g packing mixing, disinfection inoculation.
5, mycelium culture management
Culturing room's optimal temperature is 18 ~ 25 DEG C, and humidity is 70 ~ 85%, and lucifuge cultivates 8 days.Mycelia starts aerlbic culture after being covered with surface.
6, sporophore is cultivated
After by kind, treat that mycelia is covered with whole culture vessel and dark bundle arrives container bottom, start to see light, but avoid sunlight direct projection.If culturing room's light is too dark, fluorescent lamp can be supplemented and shine.After mycelia sees light, see there is orange chromogenesis on culture material surface or surrounding, and occur the orange button of grain of rice shape, namely button becomes sporophore after extending.Sporophore breeding phase, temperature should be 20 ~ 23 DEG C, and more than 25 DEG C of poor growths, humidity should bring up to 80 ~ 85%.The mycelia atrophy more than 27 DEG C can not go out bacterium bundle.In sporophore late stage of culture, strengthen circulation and the exchange of room air gradually, if culturing room's cultivation stage finds that pollution should be removed immediately.
7, gather: adopt rear mean fresh and reach 28.7 grams/cultivation box.
The thecasporous Collection and preservation of embodiment 2 Cordyceps militaris (L.) Link. (1)
(1) preparation of many spores carrier adsorption sand:
River sand 60 orders are sieved, discards macrobead and impurity, then sieve with 80 orders, remove fine sand.Suck irony with magnet, put into container 10% salt acid soak, acid of desalting after 24 hours, wash bubble with water for several times to neutral, sand is dried.
(2) sterilizing of multi spore collection container and drying treatment:
Get after the many spores carrier adsorption sand about 25g handled well mixes with equivalent distilled water and put into 250mL triangular flask, then overall 121 DEG C of moist heat sterilization 30min.Loft drier 80 DEG C of dryings 24 hours are put into until complete drying after sterilizing.
(3) many spores are separated the selection of parent's sporophore:
Choose just not polluted of Later growth in culture bottle in embodiment 1, the sporophore growth cycle is short, bar shaped neat, and even thickness, color Exocarpium Citri Rubrum, ascoma fully-developed culture bottle put into Bechtop after bottle surface sterilization.
(4) collection of many spores:
Choose ascoma fully-developed sporophore, through aseptic technique, the head scissors that ascoma is abundant is cut 1cm, put into many spores separated and collected bottle that sterilizing-drying is crossed, collect 5 for every bottle.
Room temperature places 10 days, until sporophore complete drying.In the meantime, the ascoma that a part is ripe can launch thecaspore on fine sand by nature, have the ascoma of Partial mature not yet to launch thecaspore just dry, some not full ripe ascoma does not enter the period of launching, and thecaspore is still retained in dry ascoma.
After static drying terminates, many spores separated and collected bottle is put into shaking table, carry out concussion 24 hours with the rotating speed of 80r/min.
Due to the rubbing effect of fine sand, the ascoma of dry maturation and immature ascoma receive the rubbing effect of fine sand thus broken and then release thecaspore wherein.Then the sporophore part of drying is taken out, the thecasporous sand of maturation collected the thecaspore containing the maturation of launching out and do not launch out.Finally achieve the object of the thecasporous separation of Cordyceps militaris (L.) Link., collection.The present embodiment can collect the whole thecaspores in sporophore in ascoma, and collection rate is 100%.
(5) preservation of many spores
Small test tube with 160 DEG C of 2 hours dry sterilizations, by collect containing launching out ripe thecaspore and the thecasporous sand of maturation that do not launch out is sub-packed in small test tube, then seal with solid paraffin, prevent moisture from entering, room temperature keeps in Dark Place 2 years.
The thecasporous Collection and preservation of embodiment 3 Cordyceps militaris (L.) Link. (2)
(1) preparation of many spores carrier adsorption sand:
River sand 60 orders are sieved, discards macrobead and impurity, then sieve with 80 orders, remove fine sand.Suck irony with magnet, put into container 15% salt acid soak, acid of desalting after 24 hours, wash bubble with water for several times to neutral, sand is dried.
(2) sterilizing of multi spore collection container and drying treatment:
Get after the many spores carrier adsorption sand about 50g handled well mixes with equivalent distilled water and put into 250mL triangular flask, then overall 121 DEG C of moist heat sterilization 30min.Loft drier 80 DEG C of dryings 48 hours are put into until complete drying after sterilizing.
(3) many spores are separated the selection of parent's sporophore:
To choose in embodiment 1 just not polluted of Later growth in culture bottle, the sporophore growth cycle is short, bar shaped neat, even thickness, color Exocarpium Citri Rubrum, ascoma fully-developed culture bottle put into Bechtop after bottle surface sterilization.
(4) collection of many spores:
Choose ascoma fully-developed sporophore, through aseptic technique, the head scissors that ascoma is abundant is cut 2cm, put into many spores separated and collected bottle that sterilizing-drying is crossed, approximately collect 10 for every bottle.
Room temperature places 30 days, until sporophore complete drying.In the meantime, the ascoma that a part is ripe can launch thecaspore on fine sand by nature, have the ascoma of Partial mature not yet to launch thecaspore just dry, some not full ripe ascoma does not enter the period of launching, and thecaspore is still retained in dry ascoma.
After static drying terminates, many spores separated and collected bottle is put into shaking table, carry out concussion 48 hours with the rotating speed of 200r/min.
Due to the rubbing effect of fine sand, the ascoma of dry maturation and immature ascoma receive the rubbing effect of fine sand thus broken and then release thecaspore wherein.Then the sporophore part of drying is taken out, the thecasporous sand of maturation collected the thecaspore containing the maturation of launching out and do not launch out.Finally achieve the object of the thecasporous separation of Cordyceps militaris (L.) Link., collection.The present embodiment can collect the whole thecaspores in sporophore in ascoma, and collection rate is 100%.
(5) preservation of many spores
Small test tube is with 160 DEG C of 2 hours dry sterilizations, by collect containing launching out ripe thecaspore and the thecasporous sand of maturation that do not launch out is sub-packed in small test tube, then with solid paraffin sealing, prevent moisture from entering, storage conditions is 0 DEG C of refrigeration 4 years.
The thecasporous collection of embodiment 4 Cordyceps militaris (L.) Link. (3)
(1) preparation of many spores carrier adsorption sand:
River sand 60 orders are sieved, discards macrobead and impurity, then sieve with 80 orders, remove fine sand.Suck irony with magnet, put into container 20% salt acid soak, acid of desalting after 24 hours, wash bubble with water for several times to neutral, sand is dried.
(2) sterilizing of multi spore collection container and drying treatment:
Get after the many spores carrier adsorption sand about 50g handled well mixes with equivalent distilled water and put into 500mL triangular flask, then overall 121 DEG C of moist heat sterilization 30min.Loft drier 80 DEG C of dryings 38 hours are put into until complete drying after sterilizing.
(3) many spores are separated the selection of parent's sporophore:
To choose in embodiment 1 just not polluted of Later growth in culture bottle, the sporophore growth cycle is short, bar shaped neat, even thickness, color Exocarpium Citri Rubrum, ascoma fully-developed culture bottle put into Bechtop after bottle surface sterilization.
(4) collection of many spores:
Choose ascoma fully-developed sporophore, through aseptic technique, the head scissors that ascoma is abundant is cut 2cm, put into many spores separated and collected bottle that sterilizing-drying is crossed, approximately collect 8 for every bottle.
Room temperature places 25 days, until sporophore complete drying.In the meantime, the ascoma that a part is ripe can launch thecaspore on fine sand by nature, have the ascoma of Partial mature not yet to launch thecaspore just dry, some not full ripe ascoma does not enter the period of launching, and thecaspore is still retained in dry ascoma.
After static drying terminates, many spores separated and collected bottle is put into shaking table, carry out concussion 40 hours with the rotating speed of 150r/min.
Due to the rubbing effect of fine sand, the ascoma of dry maturation and immature ascoma receive the rubbing effect of fine sand thus broken and then release thecaspore wherein.Then the sporophore part of drying is taken out, the thecasporous sand of maturation collected the thecaspore containing the maturation of launching out and do not launch out.Finally achieve the object of the thecasporous separation of Cordyceps militaris (L.) Link., collection.The present embodiment can collect the whole thecaspores in sporophore in ascoma, and collection rate reaches 100%.
(5) preservation of many spores
Small test tube is with 160 DEG C of 2 hours dry sterilizations, by collect containing launching out ripe thecaspore and the thecasporous sand of maturation that do not launch out is sub-packed in small test tube, then with solid paraffin sealing, prevent moisture from entering, storage conditions is 6 DEG C of refrigerations 3 years.
The thecasporous activation of Cordyceps militaris (L.) Link. of embodiment 5 Collection and conservation and cultivation
The Cordyceps militaris (L.) Link. thecaspore of Example 2-4 Collection and preservation, aseptically opens many spores preservation pipe, gets the part grains of sand on PDA slant medium, transfer once after growing bacterium colony again respectively.Or get the grains of sand in suitable liquid nutrient medium (formula of 1L liquid culture medium is peeled potatoes 300g(liquor), glucose 10g, peptone 2g, potassium primary phosphate 0.5g, magnesium sulfate 1g, water 1000ml) in, directly do fruiting experiment or inclined-plane of transferring again after multiplication culture.
After the Cordyceps militaris (L.) Link. thecaspore activation that embodiment 2-4 preserves, the method recorded according to embodiment 1 carries out the cultivation of Cordyccps-militaris-(L.)-link. Sporophore.Cultivation results finds, the Cordyceps militaris (L.) Link. thecaspore that embodiment 2-4 preserves all can be cultivated and obtain sporophore, adopt rear mean fresh and reach 28.7,28.9,28.8 grams/cultivation box respectively, the output of sporophore and biological character and embodiment 1 cultivate the sporophore that obtains all without significant difference, illustrate that the thecasporous method of Collection and preservation Cordyceps militaris (L.) Link. of the present invention can retain parent's inherited character.The present embodiment result illustrates: Cordyceps militaris (L.) Link. thecaspore fungi preservation 2-4 under room temperature or general refrigerator condition of the collection in above-described embodiment 2-4 still has biological activity, and fruiting body yield and biological transformation ratio are without significant difference, substantially remain the inherited character of original parent.
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (8)
1. the thecasporous collection method of Cordyceps militaris (L.) Link., is characterized in that, comprise the steps:
1) sand is put into many spores separated and collected container that can seal, sterilizing, drying;
2) Cordyceps militaris (L.) Link. ascoma fully-developed, dry sporophore are put into step 1) many spores separated and collected container;
3) many spores separated and collected of vibrating container, the thecasporous sand of maturation collected the thecaspore containing the maturation of launching out and do not launch out; Wherein, step 3) described in vibration be with 80-200r/min speed oscillation 24-48h by many spores separated and collected container.
2. collection method as claimed in claim 1, is characterized in that, described step 1) sand is neutral dry sand, it obtains by the following method:
A) river sand is crossed 60 mesh sieves, discard macrobead and impurity;
B) sieve with 80 orders again, discard fine sand, collect unsifted sand;
C) irony in sand is removed;
D) with after the salt acid soak sand 24h of 10%-20%, acid of desalting, washes bubble with water to neutral, dries sand.
3. collection method as claimed in claim 1, it is characterized in that, step 1) described in the sand laying method of putting into many spores separated and collected container that can seal be by etc. after the sand of quality mixes with distilled water, 1:3-10 is placed in many spores separated and collected container by volume.
4. collection method as claimed in claim 1, is characterized in that, step 1) described in sterilizing be 121 DEG C, moist heat sterilization 30min.
5. collection method as claimed in claim 1, is characterized in that, step 1) described in drying be 80 DEG C of dry 24-48h.
6. collection method as claimed in claim 1, is characterized in that, step 2) described in sporophore put into many spores separated and collected container, the sporophore number put into and the mass ratio of container sand are 1: 2.5-5g.
7. the collection method as described in as arbitrary in claim 1-6, is characterized in that, step 3) in also comprise the sporophore of drying taken out.
8. the arbitrary described application of thecasporous collection method in Cordyceps militaris (L.) Link. breeding of claim 1-6.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210282858.2A CN103571788B (en) | 2012-08-09 | 2012-08-09 | A kind of thecasporous Collection and preservation method of Cordyceps militaris (L.) Link. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210282858.2A CN103571788B (en) | 2012-08-09 | 2012-08-09 | A kind of thecasporous Collection and preservation method of Cordyceps militaris (L.) Link. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103571788A CN103571788A (en) | 2014-02-12 |
| CN103571788B true CN103571788B (en) | 2015-11-25 |
Family
ID=50044503
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210282858.2A Active CN103571788B (en) | 2012-08-09 | 2012-08-09 | A kind of thecasporous Collection and preservation method of Cordyceps militaris (L.) Link. |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103571788B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107955794B (en) * | 2017-11-27 | 2021-04-13 | 沈阳农业大学 | High-quality preservation method of cordyceps militaris strains |
| CN108739053A (en) * | 2018-06-22 | 2018-11-06 | 辽东学院 | A kind of method of long-term preservation high cordycepin Cordyceps militaris spawn |
| CN109122049A (en) * | 2018-08-03 | 2019-01-04 | 内蒙古农业大学 | A kind of more spore separators of Cordyceps militaris and method |
| CN117063780B (en) * | 2023-10-17 | 2023-12-26 | 四川朕源生物科技有限公司 | Cordyceps sinensis ascospore harvesting culture medium and harvesting method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1676598A (en) * | 2004-03-29 | 2005-10-05 | 龙泉市兴龙科技开发研究所 | Glassy ganoderm spore powder breeding and collecting technique |
| CN101748073A (en) * | 2008-12-18 | 2010-06-23 | 北京农业生物技术研究中心 | Method for separating and preserving Cordyceps militaris spawn |
| CN101861795A (en) * | 2010-05-10 | 2010-10-20 | 云南农业大学 | Method for promoting propagation of tricholoma matsutake artificially |
-
2012
- 2012-08-09 CN CN201210282858.2A patent/CN103571788B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1676598A (en) * | 2004-03-29 | 2005-10-05 | 龙泉市兴龙科技开发研究所 | Glassy ganoderm spore powder breeding and collecting technique |
| CN101748073A (en) * | 2008-12-18 | 2010-06-23 | 北京农业生物技术研究中心 | Method for separating and preserving Cordyceps militaris spawn |
| CN101861795A (en) * | 2010-05-10 | 2010-10-20 | 云南农业大学 | Method for promoting propagation of tricholoma matsutake artificially |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103571788A (en) | 2014-02-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100469867C (en) | Static culture technology of cordycep liquid | |
| CN101215527A (en) | Method for cultivating silkworm chrysalis Cordyceps sinensis | |
| CN101473808A (en) | Artificial culture, breeding and preservation method of plant root-knot nematode | |
| CN103404364A (en) | Grifola frondosa liquid culture cultivating and high-yield cultivating method | |
| CN102612966B (en) | Method for obtaining dendrobium aphyllum seeds to germinate effective symbiotic fungi | |
| CN107034147A (en) | The selection and cultural method of flat mushroom kind are planted for industrial bottle | |
| WO2014010314A1 (en) | Method for cultivating mushrooms using reusable fiber substrate, and culture medium for cultivation using same | |
| CN105660191A (en) | Amauroderma rugosum sporocarp culture method | |
| CN104686196B (en) | Method for preserving and separating toadstool strain through sporocarp dried in shade | |
| CN103843583A (en) | Industrialized production method of green cordyceps militaris | |
| CN106635884B (en) | The separation and its application of a kind of Kiwi berry root endogeny rayungus | |
| CN103571788B (en) | A kind of thecasporous Collection and preservation method of Cordyceps militaris (L.) Link. | |
| CN112021068B (en) | Rejuvenation method of tremella aurantialba sporocarp strains | |
| CN101333550B (en) | Method for preparing cyclic dipeptides compounds and use thereof | |
| CN102630534B (en) | Method for controlling pine wood nematode diseases by utilizing body surface bacteria of American pine wood nematodes | |
| CN101874453A (en) | Culture method of snow white mushroom strains and sporocarps | |
| CN110140591A (en) | Hickory chick superior strain and its application | |
| CN106434349A (en) | Method for collecting mycorrhizal fungi through using tissue culture seedling of sterile blueberry | |
| CN103891526A (en) | Method for cultivating bud-thinning-free pleurotus eryngii cultivated in bags | |
| CN100574614C (en) | A kind of calla lily germplasm resources in vitro conservation method | |
| JP2017038541A (en) | Cultivation method of boletus | |
| CN112442449B (en) | Ramaria original strain culture medium and application thereof as well as Ramaria original strain and culture method thereof | |
| CN105103947A (en) | Using method of special bacterium source formatted by ectotrophic mycorrhiza under natural forest | |
| CN101748073B (en) | Method for separating and preserving Cordyceps militaris spawn | |
| CN116396869A (en) | Domesticated strain of Morchella and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 100195 Beijing city Haidian District West Fourth Ring Road No. 15 East Building Room 307 Patentee after: Beijing Zhongyi Yuanda Agriculture Development Co. Ltd. Address before: 100195 Beijing city Haidian District West Fourth Ring Road No. 15 East Building Room 307 Patentee before: BEIJING ZHONGYI YUANDA MECHANICAL AND ELECTRICAL EQUIPMENT CO., LTD. |