JP3879427B2

JP3879427B2 - A method for preparing the scallop that does not exhibit short-term reproductive dormancy and a pest control method using the same

Info

Publication number: JP3879427B2
Application number: JP2001112437A
Authority: JP
Inventors: 美徳庄野; 昇浮城
Original assignee: Sumitomo Chemical Co Ltd
Current assignee: Sumitomo Chemical Co Ltd
Priority date: 2001-04-11
Filing date: 2001-04-11
Publication date: 2007-02-14
Anticipated expiration: 2018-03-13
Also published as: JP2001346481A

Description

【０００１】
【発明の属する技術分野】
本発明は温室等の施設における栽培時に問題となる微小害虫の天敵昆虫として有用なヒメハナカメムシ、その調製方法、およびそれを用いる害虫防除方法に関する。
【０００２】
【従来の技術】
ヒメハナカメムシ（Orius属）は、難防除害虫であるアザミウマ類などの優れた天敵として知られており、かかる害虫の防除方法として、ナミヒメハナカメムシ、タイリクヒメハナカメムシ等の利用が試みられている。
これらのヒメハナカメムシの天敵昆虫としての活動期間は僅かに1ヶ月程度であり、また、中秋季〜早春季の短日条件下に幼虫時代を経過した成虫の産卵率が激減する、いわゆる、短日下生殖休眠性を呈するために、晩秋季から早春季にかけては、ハウス内においても、その正常な世代交代が行われない。その結果、晩秋季〜早春季における花卉、蔬菜、果樹等のハウス栽培が一層拡大しつつある昨今の状況の中で、当該時期においては、このようなヒメハナカメムシによる害虫防除が実質上できなくなってしまうという問題があった。
【０００３】
【発明が解決しようとする課題】
本発明の目的は短日下生殖休眠性を呈さないヒメハナカメムシおよびその選択飼育方法を提供することにある。
本発明の他の目的は、かかる短日下生殖休眠性を呈さないヒメハナカメムシを用いる害虫の防除方法を提供することにある。
【０００４】
【課題を解決するための手段】
本発明者らは、ヒメハナカメムシを用いる害虫防除について検討を重ねた結果、短日下生殖休眠性ヒメハナカメムシの飼育を短日条件下で累代にわたって行うことにより、短日下においても、その生殖休眠性を呈さないヒメハナカメムシが得られること、およびこのようにして調製されるヒメハナカメムシが秋季、冬季ないし春季の短日条件下での作物栽培時の害虫防除に優れた効果を発揮することを見いだし本発明に至った。
【０００５】
即ち、本発明は、短日下生殖休眠性を呈さないヒメハナカメムシ、その調製法としての、(1)短日下生殖休眠性ヒメハナカメムシを短日条件下飼育し、該ヒメハナカメムシ成虫に産卵させた後、(2)該卵を短日条件下飼育し、得られる成虫に産卵させる工程を繰り返すことにより累代飼育を行うことによる短日下生殖休眠性を呈さないヒメハナカメムシの調製方法および、作物栽培地域に短日下生殖休眠性を呈さないヒメハナカメムシを放飼することによる害虫防除方法に関するものである。
【０００６】
【発明の実施の形態】
以下、本発明を詳細に説明する。
本発明において、「短日条件下飼育」とは、一日当たりの昼時間（明期）が１２時間未満（夜時間（暗期）が１２時間以上）の条件での飼育を指し、自然環境における中秋季〜早春季にかけての条件下での飼育がこれに当たる。
また、「ヒメハナカメムシの飼育」とは、ヒメハナカメムシの卵、幼虫または成虫を飼い育てることをいい、「成虫に産卵させる」とは、成虫を飼育することによりその雌に卵を産ませることをいう。
【０００７】
本発明に使用されるヒメハナカメムシとしては通常、主として温帯地域に分布し短日下生殖休眠性を呈するものを挙げることができ、例えば、ナミヒメハナカメムシ、タイリクヒメハナカメムシ、ツヤヒメハナカメムシ、コヒメハナカメムシを挙げることができる。
短日下生殖休眠性のヒメハナカメムシはその種類、産地等により異なるが、長日条件下で幼虫時代を経過した成虫に比べ産卵雌率（雌成虫数に対する産卵した雌成虫数の割合）が一般的には、１／２〜１／１０程度となるものである。
【０００８】
飼育は通常、明期／暗期、温度、湿度等が調節可能な恒温恒湿器中に設置された飼育器中で行われ、かかる飼育器としては例えば、透明樹脂容器、透明ガラス容器等を挙げることができる。
飼育における短日条件は、自然光を用いて行うこともできるが、一般には蛍光燈、白熱電球等の室内照明などの人工照明を用いて、通常は、１日当たりの暗期が１２〜１８時間、好ましくは１３〜１６時間の範囲に調整して行われる。
飼育の温度は通常、１８〜３０℃、好ましくは２０〜２８℃である。湿度は通常、相対湿度２０〜８０％、好ましくは３０〜７０％である。
【０００９】
飼育における餌としては、例えば、スジコナマダラメイガ卵、コナダニ類、スリップス類、ハダニ類を挙げることができる。餌としてスジコナマダラメイガ卵等を与える際には予め殺卵処理したものを与えることが飼育環境を保つという点からは好ましい。また、ヒメハナカメムシへの給水方法としては、例えば、水分を含ませた脱脂綿を飼育器内に置いておく方法を挙げることができる。
飼育においてシェルターを用いることは共食い防止のために好ましく、その素材としては、種もみ、発泡プラスチック、ろ紙、段ボール等を挙げることができる。
【００１０】
ヒメハナカメムシの卵から成虫までの飼育及び、成虫による産卵時の飼育を同一飼育器内で行うこともできるが、ヒメハナカメムシの世代管理、飼育環境の保全等の点からは両者を分離して行うこと及び、世代別に飼育、産卵を行わせることが好ましい。このような態様としては、例えば、ある世代の卵を飼育器内に接種し、飼育して成虫となし、得られる成虫を別の飼育器に移し、飼育、産卵後、採卵し、飼育器の容量、卵の成虫化率等を勘案して採卵したものの全部または一部を、また別の飼育器中で飼育する方法を挙げることができる。
【００１１】
また、成虫の産卵においては、産卵用基材を飼育器内に置いておくことが好ましく、かかる基材としては、大豆、ソラマメ等の幼苗、ゼラニウム等の葉などを挙げることができる。
【００１２】
短日下生殖休眠性ヒメハナカメムシから短日下生殖休眠性を呈さないヒメハナカメムシを調製するのに要する期間は、その種類、産地等により異なるが、通常は約6ヶ月〜３年程度である。該生殖休眠性を呈さないヒメハナカメムシの確立は、明期１０時間／暗期１４4時間、温度２５℃条件で飼育、産卵させたときの産卵雌率（雌成虫数に対する産卵した雌成虫数の割合）を調査し、その比率が約６０％以上となることにより確認できる。
【００１３】
かくして得られる短日下生殖休眠性を呈さないヒメハナカメムシは、例えばピーマン、キュウリ、ナス、イチゴ、トマト等の果樹、蔬菜、花卉等の栽培、特にそれらのハウス栽培において、アザミウマ類、ダニ類、アブラムシ類等の害虫の防除、好ましくはアザミウマ類の防除に適用され、殊に、通常の短日下生殖休眠性の系統においては害虫防除ができなくなる晩秋季から冬季さらには早春季において有用となる。
害虫防除は通常、作物植物を定植後、または作物植物が発芽後、栽培地域内、例えばハウス内に、該短日下生殖休眠性を呈さないヒメハナカメムシ成虫を放飼することにより行われる。
放飼する該ヒメハナカメムシの数は害虫の発生状況、作物植物の密集度等を考慮して適宜決定される。
【００１４】
尚、該ヒメハナカメムシの生育に影響のない範囲において、例えばピリプロキシフェン、ブプロフェジン、ピメトロジン、アミトラズ、ミルベメクチン等の選択性殺虫剤、殺ダニ剤；ＢＴ剤、ボーベリア、バーティシリウム等の微生物殺虫剤；多くの殺菌剤等の化学防除剤を併用してもよい。また、オンシツツヤコバチ、チリカブリダニ、コレマニアブラバチ、ディグリファスイサエア（Digluphus isaea)、ダクヌーサシビリカ (Dacunusa sibirica)、ヤマトクサカゲロウ、ククメリスカブリダニ、デジェネレンスカブリダニ等の生物（天敵）農薬を併用することもできる。
【００１５】
以下、実施例及び試験例により、本発明をより詳細に説明するが、本発明は実施例、試験例のみに限定されるものではない。
【００１６】
参考例１
１９９５年１０月初旬に和歌山県那賀郡において、セイタカアワダチソウ上からタイリクヒメハナカメムシを約１００頭採集した。それに由来する原系統を確立し、長日（明期１４時間、暗期１０時間）、温度２５℃、湿度６０％の条件下で累代飼育を行っている。飼育方法は、次の通りである。
大型樹脂製容器（６００−９００ｍｌ）中に、水分を含ませた脱脂綿を入れ、その上に種もみ、または発泡スチロール小片を１００−２００ｍｌ入れる。そこに大豆幼苗に産卵させたタイリクヒメハナカメムシの卵をカップ当たり約５０−１００卵接種する。その後紫外線で殺卵処理を行ったスジコナマダラメイガ卵１回当たり約２００mgを週３回給餌して、長日（明期１４時間、暗期１０時間）、温度２５℃、湿度６０％の条件で約３週間飼育すると、次世代成虫が得られる。
次世代成虫を吸虫管にて採集し、前述と同じ方法で作成した別の大型樹脂容器に約５０頭放虫する。容器中には前述の殺卵済みスジコナマダラメイガ卵約２００ｍｇを給餌する。その中に大豆幼苗を入れ、前記と同じ条件で飼育すると、その茎の組織内に雌成虫が産卵を行う。３−４日毎に、産卵された大豆幼苗を取り出し、新しい幼苗と取り替え、採卵を約２〜３週間続ける。スジコナマダラメイガ卵の給餌も大豆幼苗の取り替えと同時に行う。
取り出した産卵された幼苗は、５０−１００卵ずつまとめて、前述と同様な方法で新たに作成した大型樹脂容器に入れ、次々世代の飼育を開始する。
以上の操作を繰り返し行う。
【００１７】
実施例１
(1)１９９６年４月より、参考例１に記載の方法で調製されたタイリクヒメハナカメムシを短日（明期１０時間、暗期１４時間）条件で累代飼育を行う。温度、湿度条件、および飼育方法については参考例１の場合と同様。１９９７年４月の時点においては産卵雌率が６０％を越えており、新系統が確立していることを確認した。確認方法を下記する。
【００１８】
(2)大型樹脂製容器（９００ｍｌ）中に、水分を含ませた脱脂綿を入れ、その上に発泡スチロール小片を２００ｍｌ入れる。そこに大豆幼苗に産卵させた実施例１(1)で得られた累代飼育タイリクヒメハナカメムシ卵をカップ当たり約１００卵を接種する。その後紫外線で殺卵処理を行ったスジコナマダラメイガ卵１回当たり約２００mgを週３回給餌して、短日（明期１０時間、暗期１４時間）、温度２５℃、湿度６０％の条件で約３週間飼育し、次世代成虫を得る。次世代成虫を吸虫管にて採集し、雌成虫１０頭を１頭毎に直径９ｃｍのシャーレ内に放虫し、脱脂綿に水をしみ込ませたものと、大豆幼苗をいれ、殺卵済みのスジコナマダラメイガ卵少量を給餌し、短日（明期１０時間、暗期１４時間）、温度２５℃、湿度６０％の条件で３週間飼育を続ける。大豆幼苗を３−４日毎に取り替え、実体顕微鏡下で産卵数の観察を行う。大豆幼苗を取り替えるときに同時に、脱脂綿への給水及び、給餌を行う。
結果を表１に示す。
【００１９】
尚、このようにして調製される新系統は、兵庫県宝塚市高司４−２―１住友化学工業農業化学品研究所内で、実施例１(1)の条件下、累代飼育、維持されている。
【００２０】
比較例１
参考例１に記載の方法により累代飼育された原系統のタイリクヒメハナカメムシ卵約１００卵を用いて、実施例１(2)と同一条件で飼育、採卵を行い、産卵数の観察を行う。
結果を表１に示す。
【表１】

【００２１】
実施例２
１９９６年６月に兵庫県加西市のクローバ上より、ナミヒメハナカメムシを２００頭採集した。それを参考例１と同じ方法で、原系統を確立した。その後１９９６年８月から、実施例１と同じ方法で、短日条件下で飼育を開始した。１９９７年７月には産卵雌率が６０％を越えており、新系統が確立していることを確認した。
【００２２】
尚、このようにして調製された新系統は、兵庫県宝塚市高司４−２―１住友化学工業農業化学品研究所内で、実施例１(1)と同一条件下、累代飼育、維持されている。
【００２３】
試験例１
ピーマン（品種土佐ひかりＤ及び京ゆたか）を１９９７年９月２５日に、最低夜温を１８℃に設定したハウス（約２５０ｍ²）内に１２１株定植し、１１月２０日にクロロフェナピル１０％水和剤（商品名：コテツ水和剤）の２０００倍希釈液を散布し、その後ハウスを細かい網で半分に区切り２区を設定した。
【００２４】
１区（天敵放飼区）
１２月１１日に、実施例１に準じて得られるのタイリクヒメハナカメムシの新系統を２５０頭（２頭／ｍ²）放飼した。その後１２月２７日に該タイリクヒメハナカメムシ新系統をさらに２５０頭（２頭／ｍ²）放飼した。この区を天敵（タイリクヒメハナカメムシ新系統）放飼区として設定した。この区では、アザミウマ類に対する殺虫剤防除を全く行わなかった。
【００２５】
２区（慣行防除区）
１２月２６日に、殺虫剤アセタミプリド２０％水和剤（商品名：モスピラン水和剤）の４０００倍希釈液を散布した。この２区を慣行防除区として設定した。その後１月３１日にアザミウマ類用追加防除殺虫剤としてアクリナトリン３％水和剤（商品名：アーデント水和剤）の１０００倍希釈液を散布した。
両区から収穫された、任意のピーマン５０果の被害果数を観察して、被害果率を求めた。結果を表２に示す。
【表２】

【００２６】
上記に示すように、タイリクヒメハナカメムシの新系統は、冬季短日条件下のハウス内においても、ピーマンに寄生するアザミウマ類（主としてミカンキイロアザミウマ）対し高い防除効果を長期間示した。
【００２７】
【発明の効果】
本発明によれば、短日下においても、その生殖休眠性を呈さないヒメハナカメムシが得られる。また、このようにして調製されるヒメハナカメムシを作物栽培における害虫防除に適用すると、殊に、秋季、冬季ないし春季の短日条件においても優れた害虫防除効果を発揮する。[0001]
BACKGROUND OF THE INVENTION
TECHNICAL FIELD The present invention relates to a giant bug that is useful as a natural enemy of micro-pests that are problematic during cultivation in a facility such as a greenhouse, a preparation method thereof, and a pest control method using the same.
[0002]
[Prior art]
Himehanakamemushi (Orius genus) is known as an excellent natural enemy such as thrips, which are difficult to control pests, and as a method for controlling such pests, the use of Namihimehanamemushi, Tarimikumekamemushi, etc. has been attempted Yes.
The period of activity as a natural enemy insect is only about one month, and the egg-laying rate of adults that have passed through the larval period under the short-day conditions of mid-autumn to early spring is drastically reduced. In order to exhibit the dormant reproductive dormancy, the normal generation change is not performed in the house from late autumn to early spring. As a result, in the current situation where the cultivation of flowers, sugar beets, fruit trees, etc. in the late autumn to early spring seasons is further expanding, pest control by this kind of bug is virtually impossible in this period. There was a problem that.
[0003]
[Problems to be solved by the invention]
An object of the present invention is to provide a long-horned beetle that does not exhibit short-term reproductive dormancy and a method for selectively raising the same.
It is another object of the present invention to provide a method for controlling pests using the long-horned bug that does not exhibit such short-day reproductive dormancy.
[0004]
[Means for Solving the Problems]
As a result of repeated investigations on pest control using a long-horned beetle, the present inventors conducted breeding of short-day reproductive dormant Himehana-kamemushi under short-day conditions, so that even under short days, Giant beetle that does not exhibit reproductive dormancy can be obtained, and the beetle that is prepared in this way has an excellent effect on pest control when cultivating crops under short-day conditions in autumn, winter or spring As a result, the present invention has been found.
[0005]
That is, the present invention relates to a short-day reproductive dormancy that does not exhibit short-day reproductive dormancy, and a method for preparing the same, (1) a short-day reproductive dormant long-eared stink bug is bred under short-day conditions, (2) Breeding the eggs under short-day conditions, and repeating the process of laying eggs on the resulting adults to prepare them in a short day, so that they do not exhibit short-day reproductive dormancy The present invention relates to a method and a pest control method by releasing a long-horned beetle that does not exhibit short-term reproductive dormancy in a crop cultivation area.
[0006]
DETAILED DESCRIPTION OF THE INVENTION
Hereinafter, the present invention will be described in detail.
In the present invention, “bred under short-day conditions” refers to breeding under conditions where the daytime (light period) per day is less than 12 hours (night time (dark period) is 12 hours or more), and in a natural environment. Breeding under conditions from mid-autumn to early spring corresponds to this.
Also, “bringing of the stink bugs” means rearing eggs, larvae or adults of stink bugs, and “bringing the adults” lays eggs in the female by rearing the adults. That means.
[0007]
Examples of the common beetle used in the present invention include those that are distributed mainly in temperate regions and exhibit short-day reproductive dormancy, such as Namihimehanamemushi, Tairikhimemekamemushi, Tsuyahimemekamemushi. Can be mentioned.
The short-day reproductive dormancy of the beetle is different depending on the type and place of production, but the rate of female laying eggs (ratio of the number of female laying eggs to the number of adult females) compared to adults that have passed the larval period under long-day conditions. Generally, it is about 1/2 to 1/10.
[0008]
Breeding is usually carried out in a breeder set up in a constant temperature and humidity chamber in which the light period / dark period, temperature, humidity, etc. can be adjusted. Examples of such breeders include transparent resin containers and transparent glass containers. Can be mentioned.
Short day conditions in breeding can be performed using natural light, but generally using artificial lighting such as indoor lighting such as fluorescent lamps and incandescent bulbs, the dark period per day is usually 12 to 18 hours, Preferably, the adjustment is performed in the range of 13 to 16 hours.
The breeding temperature is usually 18 to 30 ° C, preferably 20 to 28 ° C. The humidity is usually 20-80% relative humidity, preferably 30-70%.
[0009]
Examples of the food used in the breeding include a white-spotted cod egg, acarid mites, a slips, and a spider mite. It is preferable from the standpoint of keeping the breeding environment to give eggs that have been previously slaughtered when feeding eggs such as Spodoptera cristatus. Moreover, as a method for supplying water to the Japanese bug, for example, a method of placing absorbent cotton soaked with moisture in an incubator can be mentioned.
It is preferable to use a shelter in breeding to prevent cannibalism, and examples of the material include seed meal, foamed plastic, filter paper, cardboard and the like.
[0010]
Breeding from the eggs to adults of the beetle and adults can be carried out in the same incubator, but they are separated from the viewpoint of generation management of the beetle, conservation of the breeding environment, etc. It is preferable to carry out and breed and lay eggs by generation. As such an embodiment, for example, a certain generation of eggs is inoculated into an incubator, reared to become adults, the resulting adults are transferred to another incubator, and after breeding, egg laying, egg collection, A method of raising all or part of the eggs collected in consideration of the capacity, the adult rate of eggs, etc. in another incubator can be mentioned.
[0011]
In the case of adult egg laying, it is preferable to place a substrate for laying eggs in an incubator. Examples of the substrate include young seedlings such as soybeans and broad beans, leaves such as geranium, and the like.
[0012]
The time required to prepare the short-day reproductive dormancy of the short-day reproductive dormancy depends on the type and place of production, but it is usually about 6 months to 3 years. is there. The establishment of the beetle that does not exhibit reproductive dormancy is based on the ratio of the number of female laying eggs to the number of female laying eggs relative to the number of female laying eggs when bred and spawned under conditions of 10 hours light period / 144 hours dark period and temperature of 25 ° C. It can be confirmed by investigating the ratio) and the ratio is about 60% or more.
[0013]
The long-horned beetle that does not exhibit short-term reproductive dormancy obtained in this way is, for example, fruit trees such as peppers, cucumbers, eggplants, strawberries, tomatoes, side dishes, flower buds, etc., especially in their house cultivation, thrips, mites It is applicable to the control of pests such as aphids, preferably thrips, and is particularly useful in late autumn, winter and even early spring when pest control is not possible in normal short-day reproductive and dormant lines. Become.
Pest control is usually carried out by releasing the adult bug, which does not exhibit reproductive dormancy in the short day, after planting the crop plant or after germination of the crop plant, for example, in a house.
The number of the bugs to be released is appropriately determined in consideration of the occurrence of pests, the density of crop plants, and the like.
[0014]
In the range that does not affect the growth of the beetle, for example, selective insecticides and acaricides such as pyriproxyfen, buprofezin, pymetrozine, amitraz, and milbemectin; microbial insecticides such as BT agent, Boberia and Verticillium Agents: Many chemical control agents such as bactericides may be used in combination. In addition, on-situ gloss Kovacs, persimilis, Kore mania bra bees, Digurifasu Isaea (Digluphus isaea), Dakunusa Shibirika (Dacunusa sibirica), Yamato lacewings, Kuku cucumeris, also be used in combination with the biological (natural enemies) agricultural chemicals such as DeGeneres Reference californicus it can.
[0015]
Hereinafter, although an example and a test example explain the present invention in detail, the present invention is not limited only to an example and a test example.
[0016]
Reference example 1
At the beginning of October 1995, about 100 head-winged beetles were collected from the top of the smelt hawk in Naka-gun, Wakayama Prefecture. The original line derived from it has been established, and it is raised for generations under conditions of long days (14 hours light period, 10 hours dark period), temperature 25 ° C. and humidity 60%. The breeding method is as follows.
In a large resin container (600-900 ml), put absorbent cotton soaked with water, and seed seeds or 100-200 ml of styrofoam pieces on it. About 50-100 eggs per cup are inoculated with the eggs of the tiger beetle spawned on soybean seedlings. After that, about 200 mg per week was fed 3 times a week, and the conditions were long temperature (14 hours light period, 10 hours dark period), temperature 25 ° C, humidity 60%. The next-generation adults can be obtained after breeding for about 3 weeks.
Next-generation adults are collected in the sucking tube, and about 50 insects are released in another large resin container prepared in the same manner as described above. The container is fed with about 200 mg of the above-mentioned egg-killed eggplant egg. If soybean seedlings are put in it and bred under the same conditions as described above, adult females lay eggs in the tissue of the stem. Every 3-4 days, the spawned soybean seedlings are removed, replaced with new seedlings, and egg collection is continued for about 2-3 weeks. Feeding the Japanese white flounder eggs at the same time as changing the soybean seedlings.
The extracted spawned seedlings are put together in 50-100 eggs and placed in a newly created large resin container by the same method as described above, and the breeding of the next generation is started.
Repeat the above operation.
[0017]
Example 1
(1) Beginning in April 1996, the tiger beetle prepared by the method described in Reference Example 1 is bred for generations on short days (light period 10 hours, dark period 14 hours). The temperature, humidity conditions, and breeding method are the same as in Reference Example 1. As of April 1997, the egg-laying female rate exceeded 60%, confirming the establishment of a new line. The confirmation method is as follows.
[0018]
(2) Put absorbent cotton soaked in water in a large resin container (900 ml), and put 200 ml of polystyrene foam on it. About 100 eggs per cup are inoculated with the successive breeding tiger beetle eggs obtained in Example 1 (1) on which soybean seedlings are laid. After that, about 200 mg per week was fed 3 times a week, and the conditions were a short day (light period 10 hours, dark period 14 hours), temperature 25 ° C. and humidity 60%. To raise the next generation adults. Next-generation adults are collected in the sucker tube, 10 adult females are released into a petri dish with a diameter of 9 cm, and each cotton-soaked cotton is soaked in water and soy seedlings are added. Feed a small amount of Sorbus mackerel eggs and continue the breeding for 3 weeks under the conditions of short day (light period 10 hours, dark period 14 hours), temperature 25 ° C. and humidity 60%. Replace soybean seedlings every 3-4 days and observe the number of eggs laid under a stereomicroscope. At the same time when the soybean seedlings are replaced, water is supplied to the cotton wool and feeding is performed.
The results are shown in Table 1.
[0019]
The new line prepared in this way is maintained and maintained under the conditions of Example 1 (1) in the Sumitomo Chemical Industry Agricultural Chemicals Research Laboratory 4-2-1 Takashi, Takarazuka, Hyogo Prefecture. .
[0020]
Comparative Example 1
Using about 100 eggs from the original strain of the tiger beetle that have been bred by the method described in Reference Example 1, the eggs are bred and collected under the same conditions as in Example 1 (2), and the number of eggs laid is observed.
The results are shown in Table 1.
[Table 1]

[0021]
Example 2
In June 1996, 200 Namihimehanakamushi were collected from clovers in Kasai City, Hyogo Prefecture. The original system was established by the same method as in Reference Example 1. Thereafter, from August 1996, breeding was started under the short day condition in the same manner as in Example 1. In July 1997, the egg-laying female rate exceeded 60%, and it was confirmed that a new line was established.
[0022]
The new line prepared in this way was raised and maintained in the same conditions as in Example 1 (1) in the Sumitomo Chemical Industries Agricultural Chemicals Research Laboratory 4-2-1 Takarazuka City, Hyogo Prefecture. Yes.
[0023]
Test example 1
Bell peppers (varieties Tosa Hikari D and Kyo Yutaka) were planted on September 25, 1997, in a house (about 250 m ² ) with a minimum night temperature set at 18 ° C., and on November 20, Chlorofenapyr 10 A 2000-fold diluted solution of% wettable powder (trade name: Kotetsu wettable powder) was sprayed, and then the house was divided in half with a fine net to set 2 sections.
[0024]
1 ward (natural enemy release zone)
On December 11, 250 (2 / m ² ) new strains of the tiger beetle obtained according to Example 1 were released. Thereafter, on December 27, 250 new mice (2 / m ² ) were released. This ward was set as a natural enemy (a new strain of the mosquito). In this section, no insecticide control was done against thrips.
[0025]
2 wards (conventional control ward)
On December 26, a 4000-fold diluted solution of the insecticide acetamiprid 20% wettable powder (trade name: Mospiran wettable powder) was sprayed. These two districts were set as customary control zones. Then, on January 31, a 1000-fold diluted solution of acrinatriline 3% wettable powder (trade name: Ardent wettable powder) was sprayed as an additional control insecticide for thrips.
The number of damaged fruits of 50 arbitrary peppers harvested from both districts was observed to determine the rate of damaged fruits. The results are shown in Table 2.
[Table 2]

[0026]
As described above, the new strain of the tiger beetle showed a high control effect over a long period of time against the thrips parasitic on bell peppers (mainly Citrus thrips) even in the house under winter short day conditions.
[0027]
【The invention's effect】
According to the present invention, a rotifer which does not exhibit reproductive dormancy even under short days is obtained. In addition, when the insect worms thus prepared are applied to pest control in crop cultivation, they exhibit an excellent pest control effect especially in the short days of autumn, winter or spring.

Claims

(1) Breeding short-day reproductive diapause beetle beetle under short-day conditions, and allowing the adult beetle to lay eggs,
(2) A method for preparing a long-horned beetle that does not exhibit reproductive dormancy in short days, wherein the eggs are bred under short-day conditions and the breeding is repeated by repeating the steps of allowing the resulting adult to lay eggs.

A method for controlling pests, characterized by releasing a long-nosed beetle that does not exhibit reproductive dormancy in a crop cultivation area.

(1) Breeding short-day reproductive diapause beetle beetle under short-day conditions, and allowing the adult beetle to lay eggs,
(2) The long-tailed beetle that does not exhibit short-day reproductive dormancy prepared by a method of successive breeding by repeating the steps of breeding the egg under short-day conditions and allowing the resulting adult to lay eggs is used. the method of.

The method according to claim 2 or 3, wherein the crop cultivation area is indoors.

The pest control method according to any one of claims 2 to 4, which is applied to crop cultivation in autumn, winter or spring.