CN109329221B - Large-scale breeding method of orius sauteri - Google Patents
Large-scale breeding method of orius sauteri Download PDFInfo
- Publication number
- CN109329221B CN109329221B CN201811483238.9A CN201811483238A CN109329221B CN 109329221 B CN109329221 B CN 109329221B CN 201811483238 A CN201811483238 A CN 201811483238A CN 109329221 B CN109329221 B CN 109329221B
- Authority
- CN
- China
- Prior art keywords
- orius
- eggs
- cage
- spawning
- filter paper
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000009395 breeding Methods 0.000 title claims abstract description 61
- 241001635523 Orius sauteri Species 0.000 title claims abstract description 24
- 235000013601 eggs Nutrition 0.000 claims abstract description 76
- 239000000758 substrate Substances 0.000 claims abstract description 55
- 230000017448 oviposition Effects 0.000 claims abstract description 43
- 241001635528 Orius minutus Species 0.000 claims abstract description 38
- 230000001488 breeding effect Effects 0.000 claims abstract description 37
- 241000209140 Triticum Species 0.000 claims abstract description 30
- 235000021307 Triticum Nutrition 0.000 claims abstract description 30
- 238000000034 method Methods 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 54
- 229920000742 Cotton Polymers 0.000 claims description 30
- 241001635529 Orius Species 0.000 claims description 14
- 238000005192 partition Methods 0.000 claims description 10
- 241000819999 Nymphes Species 0.000 claims description 9
- 239000011159 matrix material Substances 0.000 claims description 9
- 238000002955 isolation Methods 0.000 claims description 8
- 235000013339 cereals Nutrition 0.000 claims description 5
- 239000012266 salt solution Substances 0.000 claims description 4
- 229920006395 saturated elastomer Polymers 0.000 claims description 4
- 241001641310 Cunea Species 0.000 claims description 2
- 238000011534 incubation Methods 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 230000003020 moisturizing effect Effects 0.000 claims description 2
- 241000238631 Hexapoda Species 0.000 abstract description 8
- 230000004083 survival effect Effects 0.000 abstract description 5
- 241001414825 Miridae Species 0.000 description 10
- 230000000384 rearing effect Effects 0.000 description 8
- 241000500437 Plutella xylostella Species 0.000 description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical class [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 244000046052 Phaseolus vulgaris Species 0.000 description 5
- 230000012447 hatching Effects 0.000 description 5
- 241000607479 Yersinia pestis Species 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- -1 saturated saline solution (saturated potassium sulfate Chemical class 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 241001124076 Aphididae Species 0.000 description 2
- 241000320508 Pentatomidae Species 0.000 description 2
- 241001454295 Tetranychidae Species 0.000 description 2
- 241001414989 Thysanoptera Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 230000007480 spreading Effects 0.000 description 2
- 241000238421 Arthropoda Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000255967 Helicoverpa zea Species 0.000 description 1
- 241000258937 Hemiptera Species 0.000 description 1
- 241000255777 Lepidoptera Species 0.000 description 1
- 241001414826 Lygus Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 241000500441 Plutellidae Species 0.000 description 1
- 241000694540 Pluvialis Species 0.000 description 1
- 241000256626 Pterygota <winged insects> Species 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 238000003763 carbonization Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 235000021332 kidney beans Nutrition 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Images
Classifications
-
- A01K67/033—
Landscapes
- Housing For Livestock And Birds (AREA)
- Farming Of Fish And Shellfish (AREA)
- Artificial Fish Reefs (AREA)
Abstract
The invention belongs to the technical field of insect feeding, and particularly relates to a large-scale breeding method of orius minutus. According to the scale breeding method of the orius sauteri, disclosed by the invention, the wheat moth eggs are used as a main feed, the three layers of filter paper are used as main egg laying substrates, and the orius sauteri is bred by respectively separating and breeding the eggs, nymphs and adults, so that the orius sauteri is high in egg laying amount and survival rate of adults, low-cost, high-efficiency and scale breeding of the orius sauteri can be realized, and the method is suitable for industrial application.
Description
Technical Field
The invention belongs to the technical field of insect feeding, and particularly relates to a large-scale breeding method of orius minutus.
Background
Orius Orius of the genus Orius, is a natural enemy insect widely distributed in northern China. The east Asian orius pollidii can prey on various agricultural and forestry pests, nymphs and adults can prey on a large number of small agricultural and forestry pests such as thrips, aphids, whiteflies, spider mites and the like, and eggs of lepidoptera pests (such as cotton bollworms, diamond back moths and the like) can also be prey on, so that the east Asian orius pollidii has great application value in comprehensive control of the pests, and the east Asian orius pollidii is subjected to commercial production at home and abroad. However, the existing artificial feeding technology and experience of orius minutus in China are quite insufficient, and a considerable gap exists between the artificial feeding technology and the experience and the large-scale production and application.
The artificial feeding process of the orius minutus mainly comprises the processes of spawning, egg hatching, nymph and adult development and the like. According to the research, the large-scale breeding of orius minutus in east asia is under two crucial conditions: on one hand, the feed is used, living feeds are mostly used at present in China, and the living feeds mainly comprise small arthropods such as aphids, thrips, spider mites and the like; on the other hand, the spawning substrate is the spawning substrate, and young living plant buds and stems (such as bean sprouts, kidney bean seedling stems and the like) are mostly adopted at present. Both the feed and the oviposition substrate have the defects of high production condition, high cost, low yield, long production period and the like.
In addition, the raising density of the orius minutus and the humidity in the raising container are all key factors influencing the development rate, the service life, the death rate, the adult acquisition rate and the egg laying amount of adults of the orius minutus. If the breeding container is dry, the death rate of the east Asian orius minutus eggs and the nymphs of the young adults is greatly increased, but the humidity of the breeding container is too high, water drops can be condensed on the wall of the breeding container, the east Asian orius minutus is easily stuck and even drowned, and meanwhile, the feed and the spawning substrate are easily rotted and mildewed due to high humidity, so that mildew spots on the wall of the breeding container are caused. The disadvantages limit the large-scale breeding of the orius minutus from various aspects and also restrict the industrial application of the orius minutus.
Disclosure of Invention
Therefore, the technical problem to be solved by the invention is to provide a large-scale breeding method of orius sauteri, which has the advantages of low cost and high efficiency and solves the problem that large-scale artificial breeding of orius sauteri is difficult in the prior art.
In order to solve the technical problems, the invention discloses a large-scale breeding method of orius sauteri.
Specifically, the scale breeding method of orius minutus comprises the following steps:
(1) paving absorbent cotton in a selected breeding container to serve as a moisturizing material, covering filter paper on the surface of the absorbent cotton, and then filling water into the absorbent cotton to keep the filter paper in a wet state to form the spawning substrate;
(2) placing the oviposition substrate in a oviposition cage, placing the adult orius miniatus into the oviposition cage, and feeding the carpesia cunea eggs in time for feeding;
(3) placing the oviposition substrate with the orius sauteri eggs into an incubator, filling saturated salt solution at the bottom of the incubator, and starting incubation of the eggs after 2-3 days;
(4) and when the eggs begin to hatch, transferring the oviposition matrix into a nymph breeding device, and breeding by feeding fresh wheat moth eggs until the eggs grow to the old nymphs and adults and discharging the products.
In the step (1), the filter paper is three layers of filter paper.
In the step (1), the feeding container is of a U-shaped groove structure, and the size of the feeding container is as follows: the length is 8-12cm, the width is 0.5-2cm, and the height is 0.3-0.8 cm.
In the step (2), before the spawning substrate is placed in a spawning cage, a step of paving an isolation medium in the spawning cage is further included.
The isolation medium comprises sawdust and/or carbonized wheat grains, and preferably, the laying thickness of the isolation medium is 2-3 cm.
And (2) replacing oviposition matrixes every 1-3 days and feeding the moths in time.
In the step (3), a step of disposing a mesh partition above the saturated salt solution is further included, and the spawning substrate is placed on the mesh partition.
In the step (4), the nymph breeding device comprises a product bottle or a breeding cage.
In the step (4), the nymph breeding step comprises breeding to form products or breeding to preserve seeds.
The feeding of nymphs in product bottles is completed in the form of feeding products, namely, 500ml of transparent plastic bottles are selected as product bottles, air holes are formed in the bottle caps, a certain amount of sawdust is filled in the bottles, a certain amount of eggs are placed in the bottles, and the products (100 + 200 adults) are produced when the nymphs and adults grow.
The breeding mode of breeding and breed conservation is that nymphs are bred in a breeding cage to keep the population quantity, namely when eggs begin to hatch, fresh wheat moth eggs are evenly fed into the breeding cage, and the fresh wheat moth eggs are fed every 2-3d, when 30-40% of small stinkbugs in the cage emerge into adults, the small stinkbugs are collected and transferred into an egg laying cage.
According to the scale breeding method of the orius sauteri, disclosed by the invention, the wheat moth eggs are used as a main feed, the three layers of filter paper are used as main egg laying substrates, and the orius sauteri is bred by respectively separating and breeding the eggs, nymphs and adults, so that the orius sauteri is high in egg laying amount and survival rate of adults, low-cost, high-efficiency and scale breeding of the orius sauteri can be realized, and the method is suitable for industrial application.
The scale breeding method of the orius minutus is characterized in that the orius minutus is bred by adopting an insect cage suitable for scale breeding of the orius minutus, the insect cage is made of PVC plates, and the insect cage is moderate in weight and convenient to carry; meanwhile, the sealing effect is good, and the light transmission is strong; the two side surfaces of the cage body are distributed with air holes and are sealed by gauze, so that the air permeability of the cage body is enhanced without damaging the sealing degree. The drawer provided with the insect cage can be pulled out, so that the egg laying medium can be conveniently replaced; the arrangement of the egg platform can prevent the wheat moth eggs from being damped and mildewed; the arrangement of the water tank and the water injector can ensure the humidity environment in the cage body, and a suitable place is provided for breeding orius minutus.
Drawings
In order that the present disclosure may be more readily and clearly understood, the following detailed description of the present disclosure is provided in connection with specific embodiments thereof and the accompanying drawings, in which,
FIG. 1 is a front view of the structure of the rearing device of the present invention;
FIG. 2 is a right side view of the feeding apparatus of the present invention;
FIG. 3 is a top view of the drawer;
FIG. 4 is a schematic view of the assembly of the injector, sink and egg platform;
FIG. 5 is a schematic cross-sectional view of the injector, sink and egg table;
FIG. 6 is a schematic view of the water injector when water is added;
FIG. 7 is a schematic layout of a convex ring on the right side of the cage body;
FIG. 8 is a schematic view of a gauze mask;
FIGS. 9 to 11 show the results of the survival rate and the spawning rate test of orius sauteri in the breeding method according to embodiments 4 to 6 of the present invention;
the reference numbers in the figures denote: 1-cage body, 11-convex ring, 12-air hole, 2-drawer, 21-handle, 3-gauze, 31-gauze cover, 4-water injector, 41-cover plate, 42-piston rod, 43-handle, 44-piston, 45-water outlet pipe, 46-pressure valve, 47-supporting block, 5-egg table, 51-placing groove, 52-positioning groove, 6-water tank and 7-water.
Detailed Description
Example 1
The scale breeding method of the orius sauteri in the embodiment is to use wheat moth eggs as main feed, three layers of filter paper as main spawning substrates and sawdust as main isolation media, and to feed the orius sauteri by respectively separating and feeding the eggs, nymphs and adults, and the specific feeding method comprises the following steps:
(1) using a U-shaped groove with the length of 10cm, the width of 1cm and the height of 0.5cm as an oviposition substrate, laying a layer of absorbent cotton in the groove, covering the surface of the absorbent cotton with three layers of filter paper, and filling water in the absorbent cotton to keep the filter paper moist to prepare the required oviposition substrate;
(2) paving a layer of sawdust with the thickness of 2cm at the bottom of a spawning cage, then putting a proper amount of spawning substrate and a certain amount of adult small plant bugs (1 spawning substrate is put in every 100 adult small plant bugs), replacing the spawning substrate every 1-3d, and timely feeding the wheat moth eggs (0.3 ml of wheat moth eggs are put in every 100 adult small plant bugs);
(3) transferring the substrate with the orius minutus ova to an egg hatching box, arranging a certain amount of saturated saline solution (saturated potassium sulfate solution) with the volume of 1L in the box, adding 100ml of saturated saline solution, installing a partition plate with meshes above the saturated saline solution, placing the substrate with the orius minutus ova on the partition plate to keep filter paper moist, and transferring the substrate to a rearing cage when the ova begin to hatch;
(4) laying a layer of sawdust with the thickness of 2cm at the bottom of a rearing cage, feeding fresh wheat moth eggs every 2-3d, and collecting and transferring the small plant bugs to a spawning cage when 30-40% of the small plant bugs in the cage emerge into adults.
Example 2
The scale breeding method of the orius sauteri in the embodiment is to use a wheat moth egg as a main feed, three layers of filter paper as a main egg laying substrate and carbonized wheat grains as a main isolation medium, and feed the orius sauteri by respectively separating and feeding the egg, the nymph and the adult, wherein the specific feeding method comprises the following steps:
(1) using a U-shaped groove with the length of 10cm, the width of 1cm and the height of 0.5cm as an oviposition substrate, laying a layer of absorbent cotton in the groove, covering the surface of the absorbent cotton with three layers of filter paper, and filling water in the absorbent cotton to keep the filter paper moist to prepare the required oviposition substrate;
(2) spreading a layer of carbonized wheat grains with the thickness of 2cm at the bottom of a spawning cage (the wheat grains are carbonized at the temperature of 180 ℃ for 5 hours, and after the carbonization treatment, spreading and blowing air in a clean environment at room temperature for 5 hours), then putting a proper amount of spawning substrates and a certain amount of adult plutella xylostella (every 100 adult plutella xylostella put 1 spawning substrate), replacing the spawning substrates every 1-3 days, and timely feeding the plutella xylostella eggs (every 100 adult plutella xylostella put 0.3ml of plutella xylostella eggs);
(3) transferring the substrate with the orius minutus ova to an egg hatching box, arranging a certain amount of saturated saline solution (saturated potassium sulfate solution) with the volume of 1L in the box, adding 100ml of saturated saline solution, installing a partition plate with meshes above the saturated saline solution, placing the substrate with the orius minutus ova on the partition plate to keep filter paper moist, and transferring the substrate to a rearing cage when the ova begin to hatch;
(4) laying a layer of sawdust with the thickness of 2cm at the bottom of a rearing cage, feeding fresh wheat moth eggs every 2-3d, and collecting and transferring the small plant bugs to a spawning cage when 30-40% of the small plant bugs in the cage emerge into adults.
Example 3
The large-scale breeding method of orius nudus in the embodiment is characterized in that the orius nudus is bred by taking a wheat moth egg as a main feed, taking three layers of filter paper as a main oviposition matrix and taking sawdust as a main isolation medium and respectively separating and breeding the egg, the nymph and the adult and directly producing the product, and the specific breeding method comprises the following steps:
(1) using a U-shaped groove with the length of 10cm, the width of 1cm and the height of 0.5cm as an oviposition substrate, laying a layer of absorbent cotton in the groove, covering the surface of the absorbent cotton with three layers of filter paper, and filling water in the absorbent cotton to keep the filter paper moist to prepare the required oviposition substrate;
(2) paving a layer of sawdust with the thickness of 2cm at the bottom of a spawning cage, then putting a proper amount of spawning substrate and a certain amount of adult small plant bugs (1 spawning substrate is put in every 100 adult small plant bugs), replacing the spawning substrate every 1-3d, and timely feeding the wheat moth eggs (0.1-0.3 ml of wheat moth eggs are put in every 100 adult small plant bugs);
(3) transferring the substrate with the orius minutus ova to an egg hatching box, arranging a certain amount of saturated saline solution (saturated potassium sulfate solution) with the volume of 1L in the box, adding 100ml of saturated saline solution, arranging a partition plate with meshes above the saturated saline solution, placing the substrate with the orius minutus ova on the partition plate to keep filter paper moist, and placing a certain amount of ova into a product bottle when the ova begin to hatch;
(4) the product bottle is a 500ml transparent plastic wide-mouth bottle, the bottle cap is provided with air holes, a certain amount of sawdust is filled in the bottle, and the product is fed with the wheat moth eggs periodically (0.1-0.3 ml of wheat moth eggs are fed every 1-3 days) until the young nymphs and adults grow out.
Experimental example oviposition of orius minutus on different substrates
Substrate 1: taking a U-shaped groove with the length of 10cm, the width of 1cm and the height of 0.5cm as an oviposition substrate, laying a layer of absorbent cotton in the groove, covering the surface of the absorbent cotton with three layers of filter paper, and filling water into the absorbent cotton to keep the filter paper moist to prepare the required oviposition substrate.
Matrix 2: a U-shaped groove with the length of 10cm, the width of 1cm and the height of 0.5cm is used as an oviposition substrate, a layer of absorbent cotton is laid in the groove, the surface of the absorbent cotton is covered by a layer of filter paper, and water is filled in the absorbent cotton to keep the filter paper moist.
Matrix 3: a U-shaped groove with the length of 30cm, the width of 1cm and the height of 0.5cm is used as a secondary oviposition substrate, a layer of absorbent cotton is laid in the groove, the surface of the absorbent cotton is covered by three layers of filter paper, and water is filled into the absorbent cotton to keep the filter paper moist.
Matrix 4: fresh kidney beans were used as spawning substrate.
Appropriate amount of wheat moth eggs and 100 adult small-head orius bugs are placed in the spawning cage, spawning matrixes of the matrixes 1-4 are placed in the spawning cage and are randomly arranged, the spawning matrixes are taken out after 3d, the spawning amount of each matrix is observed, and the results are shown in the following table 1.
TABLE 1 oviposition of orius minutus on different substrates
| Treatment of | |
|
|
|
| Egg laying amount/ |
200 | 70 | 110 | 212 |
The result shows that the oviposition amount of the orius minutus on the oviposition substrate with three layers of filter paper, the length of 10cm and the width of 1cm is relatively large, the oviposition amount is not obviously different from the oviposition amount on the kidney bean, the oviposition substrate can be used for replacing the fresh kidney bean, and the low-cost, high-efficiency and large-scale breeding of the orius minutus is realized.
Examples 4 to 6
The scale breeding method of the orius minutus disclosed in the following embodiments 4-6 is completed in the insect cage which is suitable for scale breeding of the orius minutus shown in the attached drawings 1-8.
As shown in fig. 1-8, the insect-raising cage suitable for scale breeding of orius minutus mainly comprises a cage body 1, a drawer 2, gauze 3, a water injector 4, an egg platform 5 and a water tank 6.
As shown in fig. 1 and 2, the cage body 1 is a cuboid hollow structure, is surrounded by PVC plates, and is transparent, which is beneficial to light penetration. The length, width and/or height of the cage body 1 is about 30 cm. The left side surface and the right side surface of the cage body are both provided with air holes 12, and the air holes are arranged in a matrix form. As shown in figure 7, a plurality of convex rings 11 are arranged on the left side surface and the right side surface of the cage body, the convex rings correspond to the air holes one by one, and the convex rings and the air holes are arranged coaxially. The arrangement of the air holes can facilitate the circulation of air inside and outside the cage body, in order to prevent winged insects or orius minutus from entering and exiting the cage body through the air holes, a gauze cover 31 is arranged on the convex ring in a threaded manner, and gauze 3 is arranged on the gauze cover.
As shown in fig. 1, three drawers 2 are arranged in the inner cavity of the cage body, the three drawers are sequentially arranged from top to bottom, and the drawers can be drawn out from the front of the cage body and can also be pushed into the inner cavity of the cage body through the front of the cage body. A handle 21 is fixed to the drawer to facilitate the drawing out and pushing in of the drawer. As shown in fig. 3, a plurality of parallel egg platforms 5 are arranged on the inner side of the drawer, and a placing groove 51 is arranged on the top of each egg platform for placing the wheat moth eggs for the adult plutella xylostella to eat. The length, width and height of the egg platform 5 are respectively 20cm, 2cm and 1cm, and the wheat moth eggs are uniformly placed on the egg platform for adult plutella xylostella to eat. Positioning grooves 52 are respectively arranged at two ends of the bottom of the egg platform, as shown in fig. 4, a water tank 6 is arranged below the egg platform, absorbent cotton which completely absorbs water is paved in the water tank, and 3 layers of filter paper are paved above the absorbent cotton to keep the filter paper moist for the adult orius miniatus to lay eggs. The length, width and height of the water tank 6 are respectively 15cm, 1cm and 0.3cm, and the water tank is fixed on the drawer 2 which can be pushed and pulled at the bottom of each layer at the position 0.2cm away from the bottom. The drawer is also provided with a water injector 4, as shown in fig. 5, the water injector 4 is a cuboid hollow structure, an end cover 41 is arranged above the water injector, and the end cover is in threaded connection with the water injector. A piston rod 42 is slidably provided on the end cap, a handle 43 is fixed to the upper portion of the piston rod, and a piston 44 is fixed to the bottom of the piston rod, and the piston is placed in the inner cavity of the syringe and contacts the inner wall of the syringe. The upper portion of the outer wall of the water injector is provided with a support block 47, the inner wall of the drawer is also provided with the support block 47, the support block is of an L-shaped structure, and the support block is arranged to be in plug-in fit with the positioning groove, so that the egg platform is supported. During installation, the positioning groove on the egg platform is aligned with the supporting block, and then the supporting block is placed in the positioning groove. A water outlet pipe 45 is arranged between the lower part of the water injector and the water tank, and water in the water injector can enter the water tank through the water outlet pipe. A pressure valve 46 is arranged in the water outlet pipe, as shown in fig. 6, the end cover can be detached from the water injector, and then water 7 is added into the inner cavity of the water injector; when water needs to be added into the water tank, the handle on the end cover is pressed, the piston moves downwards at the moment, water in the inner cavity of the water injector is pressed, the pressure valve is opened when the pressure exceeds the threshold value of the pressure valve, and the water enters the water tank through the water outlet pipe. The water in the water tank can keep the humidity environment in the cage body, and is beneficial to the propagation of the orius minutus. 3 layers of filter paper are laid on the absorbent cotton, and the filter paper is kept wet for the adult orius minutus to lay eggs. When the oviposition medium is replaced each time, the whole oviposition medium is directly pulled out, and a new drawer is replaced. And putting the drawer with the eggs into a cage body without adults, feeding the wheat moth eggs after the eggs are hatched, and enabling the lygus pluvialis nymphs to grow to adults after catching the wheat moth eggs.
In examples 4 to 6 of the present invention, 200 eggs of orius minutus to be hatched were taken and placed in the above-mentioned rearing apparatus, and rearing and breeding were performed according to the IDE rearing method described in examples 1 to 3, respectively, and hatching, development, survival, and adult egg laying amount of the eggs were observed, respectively, and the results are shown in fig. 9 to 11, respectively.
From the results, the breeding of the orius sauteri is carried out by using the breeding device, basically, the eggs begin to hatch in the fourth day, and the hatchability is over 90 percent; adults begin to appear around day 15, eggs begin to lay 3-4 days after the adults, the peak of egg laying is 22-33 days, and the survival number and egg laying amount of the adults gradually decrease to zero after 40 days. The total egg production yield of 2384, 2193 and 2177 granules in the above examples 4-6 was calculated.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.
Claims (8)
1. The large-scale breeding method of the orius sauteri is characterized in that the orius sauteri eggs are used as a main feed, and the humidity of a breeding environment is controlled by an egg laying substrate containing filter paper and absorbent cotton to carry out artificial breeding on the orius sauteri;
the method comprises the following steps:
(1) paving absorbent cotton in a selected breeding container to serve as a moisturizing material, covering filter paper on the surface of the absorbent cotton, and then filling water into the absorbent cotton to keep the filter paper in a wet state to form the spawning substrate;
(2) paving an isolation medium in a spawning cage, placing the spawning substrate in the spawning cage, placing the adult orius miniatus into the spawning cage, and feeding the imagoes of the orius miniatus with the eggs of the wheat moth in time for feeding;
(3) placing the oviposition substrate with the orius sauteri eggs into an incubator, filling saturated salt solution at the bottom of the incubator, and starting incubation of the eggs after 2-3 days;
(4) when eggs begin to hatch, transferring the oviposition matrix into a nymph breeding device, and breeding by feeding fresh wheat moth eggs until the eggs grow to old nymphs and adults to produce products;
the spawning cage comprises a hollow and transparent cage body (1), air holes (12) are formed in the left side surface and the right side surface of the cage body, and the air holes (12) are arranged in a matrix form; three drawers (2) are arranged up and down in the inner cavity of the cage body, a plurality of egg platforms (5) which are arranged in parallel are arranged on the inner sides of the drawers (2), a placing groove (51) for placing a wheat moth egg is arranged at the top of each egg platform (5), and positioning grooves (52) are respectively arranged at two ends of the bottom of each egg platform (5); a water tank (6) is arranged below the egg platform (5), the absorbent cotton is placed in the water tank (6), and the filter paper is placed on the absorbent cotton; the inner side of the drawer is also provided with a water injector (4), and the water injector (4) is communicated with the water tank (6).
2. The scale breeding method of orius minutus according to claim 1, wherein in the step (1), the filter paper is three layers of filter paper.
3. The scale breeding method of orius minutus according to claim 2, wherein in the step (1), the feeding container is of a U-shaped groove structure, and the size of the feeding container is as follows: the length is 8-12cm, the width is 0.5-2cm, and the height is 0.3-0.8 cm.
4. The scale breeding method for orius minutus according to any one of claims 1 to 3, wherein the isolation medium comprises sawdust and/or carbonized wheat grains.
5. The scale breeding method of orius nudus according to claim 4, wherein the step (2) further comprises the steps of replacing oviposition matrixes every 1-3d and feeding carpesia cunea eggs in time.
6. The scale breeding method of orius minutus according to claim 5, wherein the step (3) further comprises a step of disposing a mesh partition above the saturated salt solution, and placing the spawning substrate on the mesh partition.
7. The scale breeding method of orius nudus according to claim 6, wherein in the step (4), the nymph raising device comprises a product bottle or a raising cage.
8. The scale breeding method of orius minutus according to claim 7, wherein in the step (4), the nymph raising comprises raising to form products or raising to breed.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811483238.9A CN109329221B (en) | 2018-12-05 | 2018-12-05 | Large-scale breeding method of orius sauteri |
| FR1905375A FR3089386B3 (en) | 2018-12-05 | 2019-05-22 | Large scale rearing method of Orius sauti |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811483238.9A CN109329221B (en) | 2018-12-05 | 2018-12-05 | Large-scale breeding method of orius sauteri |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109329221A CN109329221A (en) | 2019-02-15 |
| CN109329221B true CN109329221B (en) | 2021-07-30 |
Family
ID=65297544
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811483238.9A Active CN109329221B (en) | 2018-12-05 | 2018-12-05 | Large-scale breeding method of orius sauteri |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN109329221B (en) |
| FR (1) | FR3089386B3 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109717154B (en) * | 2019-02-28 | 2024-02-20 | 新疆农业科学院植物保护研究所 | Method for artificial breeding of lygus pratensis and special breeding box |
| CN111493031A (en) * | 2020-05-18 | 2020-08-07 | 河南省农业科学院烟草研究所 | Method for reducing mortality rate of middle-aged and low-aged nymphs in raising orius sauteri |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104938429B (en) * | 2015-06-30 | 2017-04-05 | 山东省农业科学院植物保护研究所 | A kind of method for raising Orius |
| CN105075992A (en) * | 2015-07-07 | 2015-11-25 | 华中农业大学 | Method for raising natural enemy insect southern minute pirate bug indoor in great deal and raising box |
| CN106472428A (en) * | 2016-09-28 | 2017-03-08 | 天津拓华生物科技有限责任公司 | A kind of utilization fruit bat and the method for bean weevil mixing raising ladybug |
-
2018
- 2018-12-05 CN CN201811483238.9A patent/CN109329221B/en active Active
-
2019
- 2019-05-22 FR FR1905375A patent/FR3089386B3/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| FR3089386B3 (en) | 2020-12-11 |
| CN109329221A (en) | 2019-02-15 |
| FR3089386A3 (en) | 2020-06-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101569297B (en) | Breeding method of pyemotes zhonghuajia and application thereof in pest biological control | |
| CN102578051A (en) | Method for artificially feeding Chilo suppressalis in room | |
| CN104604814B (en) | Method for efficient chilo suppressalis breeding | |
| CN105284747A (en) | Method and device for indoor breeding of phauda flammans walker | |
| CN102113471B (en) | Artificial rearing method of larvae of beet armyworm | |
| CN103478117B (en) | The diffusion breeding method for releasing of a kind of harmonia axyridia control cigarette aphid | |
| CN108012995B (en) | Indoor breeding method and device for Diaphania ciliata | |
| CN102125002A (en) | Method for artificially raising plant bug parasitic wasps | |
| CN109329221B (en) | Large-scale breeding method of orius sauteri | |
| CN106900656B (en) | A kind of cigarette fleahopper industrial production method | |
| CN109984093A (en) | A kind of Groton bug rearging cage and Groton bug method for breeding | |
| CN112042598B (en) | Whole set method for breeding harmonia axyridis | |
| CN103583286A (en) | Genetic control method for tea geometrids | |
| CN107581159A (en) | A kind of figured cloth moths attracted by lamplight natural enemy hangs the artificial fecundation method of cocoon honeybee | |
| CN216722754U (en) | Device is raised to scale of harris siya | |
| CN113519468A (en) | Method for feeding phyllotreta striolata in indoor generation | |
| CN205213898U (en) | Device of laying eggs of moire ladybug | |
| CN111493031A (en) | Method for reducing mortality rate of middle-aged and low-aged nymphs in raising orius sauteri | |
| CN204070205U (en) | Food aphid cecidomyiia mature larva gathering unit | |
| CN216567798U (en) | All-insect-state feeding and automatic collecting device for pink moth | |
| CN211482530U (en) | Improve food replacement device of chilo suppressalis larva survival rate | |
| CN110122426B (en) | Method for trapping coccinella septempunctata to lay eggs | |
| CN211482531U (en) | Simple and convenient, device of high-efficient change prodenia litura larva foodstuff | |
| CN109122589B (en) | Lotus herb flea beetle stem externalization pupation device based on humidity control and experiment method | |
| CN107853253B (en) | Indoor circulating breeding device and method for macrobrachium giganteum |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |