CN108012995B - Indoor breeding method and device for Diaphania ciliata - Google Patents
Indoor breeding method and device for Diaphania ciliata Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
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Abstract
The invention relates to a method and a device for raising insects, in particular to a method and a device for raising green-wing serici medinalis, which comprises the steps of raising larvae, pupae and adults and collecting and hatching eggs, wherein the method comprises the steps of raising the larvae of the green-wing serici medinalis, raising the larvae to be pre-pupated by taking sugar-gum leaves as foodstuff, and putting the larvae into a pupation device until the larvae are pupated; identifying male and female pupae, separately placing in an eclosion box, after eclosion, placing in an adult breeding device in a pairing mode, and waiting for mating and oviposition; collecting eggs in the adult feeding device, putting the eggs into a clean round plastic culture dish with the bottom paved with moisturizing filter paper, paving tender chicle leaves in the culture dish, putting the culture dish into an artificial climate box, and paying attention to the moisturizing every day until the eggs are hatched; after the larvae are hatched, the larvae are circularly subcultured according to the steps. The invention provides an insect source with relatively consistent insect-state development progress for researching the influence of biological and non-biological factors on the survival and reproduction of the Diaphania furnacalis population.
Description
Technical Field
The invention relates to a method and a device for breeding insects, in particular to a method and a device for breeding diaphania viridea hybrid webworm indoors.
Background
Diaphania angularis (Lepidoptera, Pyralidae) is the major foliar-feeding pest of the Saccharum saccharinum Alastonia schalaris L. The harm degree of the plant disease is increased year by year, and people attract attention. The worm uses longitudinal rolling leaf sheets with silk laid by larvae to form bracts, and eating mesophyll inside the bracts, so that the bracts are strong in concealment and large in food intake, and the plant damage rate of heavy people reaches 90% -95%; the tender leaf damage rate reaches 85 percent; leads to withered and yellow branches and leaves, causes fallen leaves, causes bald branch polished rods, seriously reduces the use value, the medicinal value and the ornamental value, and influences the growth of the gummy trees and the development of biological medicine industry. Although the Diaphania furnacalis is widely distributed in China, the conditions of harmfulness of Diaphania furnacalis occur in different places due to different conditions such as air temperature and the like, generally 6-7 generations occur in one year, the harmfulness time is long, and the prevention and control difficulty is high. Seriously-harmful landscaping plants and whole leaves of street trees are all exposed to food, only dead leaves remain, and urban landscape, image and ecological benefits are seriously affected.
Studies have shown that the major contributing factors to insect population outbreaks are hosts, population bases (including overwintering and overwintering bases), and an increased number of beneficial populations (e.g., lack of effective natural enemies) (mengqing et al, progress in the study of forest plant community diversity and natural control of pests, world forestry study 2003). Firstly, species abundance and multi-degree value of a garden ecosystem are low, and planting of a large-area single tree species provides abundant food for pest populations to promote mass propagation of the pest populations, and is one of main reasons for population outbreak. Because of the limited number of species in landscaping, large-area planting of a single species provides an abundant feed for pests, contributing to rapid propagation of pest (particularly phytophagous species) populations. Secondly, the pest population outbreak is the basis for adapting to local climatic conditions and forming a certain overwintering and overwintering base. Mainly embodied in the tolerance capacity (including the duration time of the limit high/low temperature and the high/low temperature) of pest populations to the limit high/low temperature of the local area, strong tolerance capacity, high survival rate and high population base. Finally, due to the low diversity of species in landscape ecosystems, a diverse habitat and abundant food sources cannot be formed to accommodate more insect species, natural enemies, predatory arthropods, insect-feeding birds and other animal species, and the harmfulness of pests cannot be regulated or resisted through the diversity of species in the system itself. Due to the influence of limited urban greening space and the tree species selected for artificial greening, serious artificial interference in the city, narrow underground space for tree growth, poor plant nutrition caused by poor management and protection and other factors, the landscape with high species enrichment is difficult to construct, so that the urban green land system is difficult to establish stable and balanced ecological function. So that more abundant Natural enemy species cannot be attracted, and thus the pest population lacks effective Natural enemy inhibition to cause explosive growth of the pest population, Natural enemy hypothesis (bengent. progress of research on diversity of forest plant communities and Natural control of pests. world forestry research 2003) has been proposed to this scholarly. Therefore, the understanding of the influence of main and key biological factors (such as hosts, natural enemies) and non-biological factors (temperature, humidity and illumination) on the survival and reproduction of the Mucuna furnacalis linne population is the premise and the root for analyzing the population outbreak.
However, the influence of biological factors (such as hosts and natural enemies) and non-biological factors (temperature, humidity and light) on the survival and reproduction of the Mucuna furnacalis linne population needs a large number of indoor populations with more consistent insect-shaped development progress as a source of the test insects. At present, no indoor feeding technology of the Diaphania furnacalis is reported at home and abroad. In view of this, a set of indoor artificial feeding technology for the yellow mealworm walker is researched to realize long-term large-scale subculture of the test insects, so that sufficient insect sources are provided for exploring the outbreak mechanism of the green mealworm walker, and the method is very important.
Disclosure of Invention
The invention aims to provide an indoor breeding method and device for Diaphania ciliate Royle Guleri, which can breed Diaphania ciliate Royle Guleri with large quantity and relatively consistent insect-state development progress as an insect source for researching the influence of biological factors (such as hosts and natural enemies) and non-biological factors (temperature, humidity and illumination) on the survival and reproduction of Diaphania ciliate Royle Guleri population.
In order to achieve the purpose, the invention adopts the technical scheme that:
an indoor breeding method of yellow-fin silk moth comprises the breeding steps of larva, pupa, imago and egg, specifically,
the specific process from initial incubation to preputial is as follows:
spreading the chickpea leaves at the bottom of the larva breeding device, taking out the chickpea leaves fully hatched in the first hatching larva from the hatching device and covering the chickpea leaves on the larva breeding device, then moving the larva breeding device to an artificial climate box for breeding,
the foodstuff is changed once every day, when the foodstuff is changed the next day, the separation net is installed at the middle part of the larva feeding device, and on the separation net, the fresh foodstuff is flatly paved for feeding, on the third day, the separation net is taken out and the bottom insect dung of the larva feeding device is cleaned, then the insects and the foodstuff on the separation net are paved into the bottom of the larva feeding device, and the fresh foodstuff is flatly paved on the separation net, and the steps are repeated until the color of the larva turns red to be in a pre-pupa state.
The specific process of pupation is as follows:
picking the larvae in a pre-pupation state from the larva feeding device to a paper groove of a pupation device, moving the pupation device to an artificial climate box, spinning the larvae to conjugate the paper groove to make the cocoons and pupate, taking out the paper groove after the larvae in the pupation device pupate, pulling the two sides, slightly pulling the two ends to tear the cocoons, and taking out the pupas by using sterilized tweezers.
The specific process of eclosion is as follows:
distinguishing male and female pupae under a stereoscope, respectively placing the male and female pupae into two different sterilized eclosion devices, placing the pupae on moisture-preserving filter paper, and moving the eclosion devices into an artificial climate box until eclosion occurs.
The specific process from mating to spawning is as follows:
transferring the female and male worms which are just eclosion on the same day into a mating and spawning device, putting the sugarcoated leaves into the copulation and spawning device, transferring the copulation and spawning device into an artificial climate box, allowing the copulation and spawning device to freely mate, taking out the male worms when egg granules appear in the copulation and spawning device, and then taking out and replacing parts with worm eggs on the copulation and spawning device every day until spawning is finished;
the specific process from hatching to initial hatching of larvae is as follows:
placing eggs of Sesamia viridis Sesami in an incubator with moisture keeping filter paper at the bottom, spreading tender chicle leaves on the incubator, placing in a climatic chamber until the eggs hatch into larvae,
after the larvae are hatched, the larvae are circularly subcultured according to the steps.
Further, the larva breeding device comprises a first box body (6), a first box cover (5) and a separation net (8), wherein the first box cover (5) covers the top of the first box body (6), the separation net (8) is movably arranged in the middle of the first box body (6), and the mesh size of the separation net (8) is 6-8 mm.
Further, the pupation device comprises a second box body (10), a second box cover (9) and a paper groove (11), wherein the second box cover (9) covers the top of the second box body (10), the paper groove (11) is placed in the second box body (10), and the paper groove (11) is double-layered.
Further the eclosion device packages a base I (16) and a cup body (14), the cup body (14) is buckled on the base I (16) in an inverted mode, the top surface of the cup body (14) is provided with a through hole, the through hole is sealed by a gauze (15), the mesh size of the gauze (15) is 10-12 meshes, and a cotton ball (13) stained with sugar water is arranged above the through hole; the middle part of the first base (16) is sunken downwards, and the moisturizing filter paper (4) is paved on the first base (16).
Further, mating spawning device includes two bases (19), a cup body section of thick bamboo (18) and bowl cover (17), and a cup body section of thick bamboo (18) set up on two bases (19), and bowl cover (17) lid is on a cup body section of thick bamboo (18), and bowl cover (17) top surface is equipped with the through-hole, and the through-hole is sealed by gauze (15), and gauze (15) mesh size is 10-12 meshes, and the through-hole top sets up cotton balls (13) that are stained with the sweet water, and two bases (19) middle part undercut, and base two (19) upper berths have filter paper (4) of moisturizing.
6. The indoor raising method of diaphania citri francis according to claim 1, characterized in that the temperature in the artificial climate box is 25 ℃ ± 1 ℃, and the photoperiod is 14L: 10D, humidity 70% +/-10%.
The outstanding substantive features and remarkable progress of the invention are as follows:
1. the invention can provide an insect source with more consistent insect state development progress for researching the influence of biological factors (such as hosts, natural enemies and pathogenic bacteria) and non-biological factors (temperature, humidity and illumination) on the survival and the propagation of the Diaphania furnacalis population. The related feeding device has simple structure, low cost, simple operation, labor saving and recycling.
2. The invention adopts a circular plastic box as a feeding device for the larva of the diaphania punctiferalis, and the middle part of the device is provided with a detachable net. Supplementary foodstuff on the net, the larva can initiatively climb to fresh foodstuff, this contact to the polypide that not only reducible when changing the foodstuff, can also keep apart the excrement and to the pollution of foodstuff, solve the problem of dying because the contact infects pathogenic microorganism in the indoor feeding of larva, can use manpower sparingly simultaneously in a large number.
3. The pupation device adopts the double-layer paper slot to supply pre-pupation, compared with the mode of natural pupation of the pupation, the pupation device adopts conjugated leaf pupation (the pupation rate is 88 percent, the aberration rate is 1.5 percent) and pupation in soil (the pupation rate is 68 percent, the aberration rate is 7.2 percent), the pupation success rate of the device is high, the aberration rate is low (the pupation rate is 92 percent, the aberration rate is 1.1 percent), the use area is saved, and each device can contain 20 pre-pupation heads on average to carry out the pupation process. And, the design of double-deck paper groove can make the larva all can conjugate pupate about the paper groove, can not influence each other, also can not cause the cocoon room to be destroyed to transfer the cocoon many times and cause physical demands and pupate failure. In addition, the labor is saved and the mechanical damage to the user is reduced in the pupa taking process.
4. The eclosion device can provide a good eclosion place, so that wings can be successfully spread after imagoes are eclosion, and nutrition can be timely supplied. The device is suitable in size, is convenient for taking the insects, and cannot cause escape of the adults during experimental operation. Meanwhile, the system can also be used as a feeding place for short-term adults, can provide research on reproduction behaviors of adults of different ages in days, and provides data support for improvement of biological control technology.
5. The mating and spawning device of the invention provides a good mating place, adults can have a high mating rate of only 15% in a 56% mesh cage in the device, and all egg grains can be completely collected. The device can be split into three parts, an upper cover, a cup body and a lower cover, and can be freely assembled, so that the whole device can be prevented from being consumed due to the fact that the female is spawned in a certain part of the device, and cost is saved. In this device the life of the adults is long, with an average life of up to 21 days, whereas the life of adults living in the net cage is only 12 days. Therefore, the female insects with longer laying period can be obtained by using the device, more eggs are obtained, and the purpose of mass propagation is achieved.
Drawings
Fig. 1 is a schematic view of the structure of a larva raising apparatus of the present invention.
Fig. 2 is a schematic structural view of the pupation device of the present invention.
Fig. 3 is a schematic structural view of the adult eclosion apparatus of the present invention.
FIG. 4 is a schematic structural diagram of the adult mating and spawning device of the present invention.
Fig. 5 is a schematic structural view of the hatching apparatus of the present invention.
Detailed Description
The technical solution of the present invention is further illustrated by the following examples.
An indoor breeding method of yellow-fin silk moth comprises the breeding steps of larva, pupa, imago and egg, specifically,
the specific process from initial incubation to preputial is as follows:
spreading the chickpea leaves at the bottom of the larva breeding device, taking out the chickpea leaves fully hatched in the first hatching larva from the hatching device and covering the chickpea leaves on the larva breeding device, then moving the larva breeding device to an artificial climate box for breeding,
the foodstuff is changed once every day, when the foodstuff is changed the next day, the separation net is installed at the middle part of the larva feeding device, and on the separation net, the fresh foodstuff is flatly paved for feeding, on the third day, the separation net is taken out and the bottom insect dung of the larva feeding device is cleaned, then the insects and the foodstuff on the separation net are paved into the bottom of the larva feeding device, and the fresh foodstuff is flatly paved on the separation net, and the steps are repeated until the color of the larva turns red to be in a pre-pupa state.
The specific process of pupation is as follows:
picking the larva in a pre-pupation state from a larva feeding device to a paper groove of a pupation device, moving the pupation device to an artificial climate box, making the cocoon into a conjugated paper groove when spinning until pupation occurs, taking out the paper groove after the larva in the pupation device pupates, pulling the two sides, slightly pulling the two ends to tear the cocoon, and taking out the pupation by using sterilized tweezers.
The specific process of eclosion is as follows:
distinguishing male and female pupae under a stereoscope, respectively placing the male and female pupae into two different sterilized eclosion devices, placing the pupae on moisture-preserving filter paper, and moving the eclosion devices into an artificial climate box until eclosion occurs.
The specific process from mating to spawning is as follows:
transferring the female and male worms which are just eclosion on the same day into a mating and spawning device, putting the sugarcoated leaves into the copulation and spawning device, transferring the copulation and spawning device into an artificial climate box, allowing the copulation and spawning device to freely mate, taking out the male worms when egg granules appear in the copulation and spawning device, and then taking out and replacing parts with worm eggs on the copulation and spawning device every day until spawning is finished;
the specific process from hatching to initial hatching of larvae is as follows:
placing eggs of Sesamia viridis Sesami in an incubator with moisture keeping filter paper at the bottom, spreading tender chicle leaves on the incubator, placing in a climatic chamber until the eggs hatch into larvae,
after the larvae are hatched, the larvae are circularly subcultured according to the steps.
Referring to fig. 1, 1 is a culture dish, 2 is a leaf, 3 is an insect egg, 4 is a moisturizing filter paper, and 21 is a cover.
As shown in figure 2, the larva breeding device comprises a box body I6, a box cover I5 and a separation net 8, wherein the box cover I5 covers the top of the box body I6, the separation net 8 is movably arranged in the middle of the box body I6, and the mesh size of the separation net 8 is 6-8 mm.
As shown in fig. 3, the pupation device comprises a second box body 10, a second box cover 9 and a paper groove 11, wherein the second box cover 9 covers the top of the second box body 10, the paper groove 11 is placed in the second box body 10, and the paper groove 11 is double-layered.
As shown in fig. 4, the eclosion device packages a base I16 and a cup body 14, the cup body 14 is buckled on the base I16 upside down, the top surface of the cup body 14 is provided with a through hole, the through hole is sealed by a gauze 15, the mesh size of the gauze 15 is 10-12 meshes, and a cotton ball 13 stained with sugar water is arranged above the through hole; the middle part of the first base 16 is sunken downwards, and the moisturizing filter paper 4 is paved on the first base 16.
As shown in figure 5, the mating and spawning device comprises a second base 19, a cup body cylinder 18 and a cup cover 17, wherein the cup body cylinder 18 is arranged on the second base 19, the cup cover 17 covers the cup body cylinder 18, the top surface of the cup cover 17 is provided with through holes, the through holes are sealed by a gauze 15, the mesh size of the gauze 15 is 10-12 meshes, cotton balls 13 stained with sugar water are arranged above the through holes, the middle of the second base 19 is sunken downwards, and moisturizing filter paper 4 is paved on the second base 19. In the figure 20 is the adult of the Diaphania viridis Walker.
The temperature in the artificial climate box is 25 +/-1 ℃, and the photoperiod is 14L: 10D, humidity 70% +/-10%.
Example 2
An indoor breeding method of Sesamia viridissima Henryi comprises breeding larva, pupa, imago and ovum, specifically comprises breeding larva of Sesamia viridisei Henryi in larva breeding device, breeding to pre-pupa with chicle leaf as foodstuff, and placing into pupation device until pupation; identifying male and female pupae, separately placing in an eclosion box, after eclosion, placing in an adult breeding device in a pairing mode, and waiting for mating and oviposition; collecting eggs in the adult feeding device, putting the eggs into a clean round plastic box with the bottom being paved with moisturizing filter paper, paving young chicle leaves on the box, putting the box into an artificial climate box, and paying attention to the moisturizing every day until the box is incubated; after the larvae are hatched, the larvae are circularly subcultured according to the steps.
The specific process from initial incubation to pupation is as follows: spreading the chickenea as foodstuff at the bottom of the device, covering the leaves of the hatching device full of larvae (the larvae which are hatched for the first time gather on tender leaves to take food) on the foodstuff, covering the box cover, and then putting the hatching device into an artificial climate box for breeding; the temperature in the artificial climate box is 25 +/-1 ℃, and the photoperiod is 14L: 10D and humidity of 70% +/-10%; changing the foodstuff once every day, when changing the foodstuff the next day, putting the separation net, laying fresh leaves on the separation net for feeding, taking out the separation net and cleaning the feces of the insects at the bottom of the box on the third day, laying the insects and leaves on the separation net at the bottom of the box, laying the fresh leaves on the separation net, repeating the above steps until the color of the larva turns red to be in a pre-pupa state, and transferring the larva to a pupation device.
The specific process of pupation is as follows: picking the larvae in a pre-pupation state from a feeding device into double-layer paper grooves in a square pupation device by using a sterilized writing brush, placing 20 heads in each device, placing a square plastic box in an artificial climate box, and making cocoons in a spinning conjugate paper groove until the cocoons are pupated; the temperature in the artificial climate box is 25 +/-1 ℃, and the photoperiod is 14L: 10D and a humidity of 70% + -10%.
The specific process of eclosion is as follows: after the larva in the pupation device pupates, taking out the paper groove, pulling the paper groove to two sides, slightly pulling outwards to tear the cocoon, taking out the pupa by using sterilized tweezers, and distinguishing male and female under a stereoscope; putting male and female pupae into sterilized eclosion box, spreading moisture-keeping filter paper, putting cotton balls with 10% sucrose solution, and putting square plastic box into artificial climate box until eclosion; the temperature in the artificial climate box is 25 +/-1 ℃, and the photoperiod is 14L: 10D and a humidity of 70% + -10%.
The specific process from mating to spawning is as follows: placing the male and female insects which are eclosized at the same age in an adult mating device, placing 2-3 sugar gum leaves and 10% sucrose cotton balls in the adult mating device, placing a mating cup in an artificial climate box, mating freely until egg grains appear on the cup wall or the blade, taking out the male insects, replacing filter paper, the blade and the mating cup, and checking whether the adult device contains the egg grains every day; if so, the filter paper, the leaf and the mating cup are continuously replaced with new ones.
The specific process from hatching to larva is as follows: collecting filter paper and leaves with egg granules, cutting egg masses on the mating cup walls together with the cup walls, putting into a clean round plastic box with the bottom paved with moisturizing filter paper, paving tender chicle leaves on the box, and putting into a climatic chamber until the box is incubated; the temperature in the artificial climate box is 25 +/-1 ℃, and the photoperiod is 14L: 10D and the humidity is 70% +/-10%, and the hatched larvae continue to be raised for subculture circularly according to the steps.
Example 3 influence of pupation site on pupation of Diaphania nubilalis
1. Test materials:
glass petri dishes (15cm), disposable plastic cups, old leaves of the sugar-gum trees, a4 white paper slots, sand (sticky: sand 2: 1), rubber bands, scissors, tweezers and other tools.
2. The test method comprises the following steps:
temperature: 25. + -.1 ℃ RH: 70% + -5% and photoperiod L: D ═ 14: 10. Insect sources: selecting 12 heads/dish of mature larva (0.2-0.25g) with regular and healthy development level to pupate, and 5 dishes in each group.
The printing paper is folded along the long edge once by the white paper slot to obtain two layers of rectangular paper sheets, and then the printing paper is folded along the front and the back of the short edge alternately to obtain the sawtooth wave-shaped paper slot. The paper slot is in the shape of a WWW seen from the side, each slot being V-shaped. And (4) cocooning by taking the groove walls on the two sides of the V-shaped groove as supporting points. Therefore, the paper grooves are arranged in two layers, and the two V-shaped grooves on the upper layer are respectively provided with independent wall surfaces. There is also a separate wall surface in each V-groove when cocooning takes place in the lower paper groove.
If a double-layer paper groove is not used, for a single-layer paper groove, a V-shaped groove with an upward opening and an adjacent V-shaped groove with a downward opening have a common wall surface, so that the common wall surface can simultaneously support the force of two cocoons, the strength is insufficient, and the cocooning and pupating rate is low. Putting a 1.5cm double-layer paper groove into a glass culture dish, and putting the old larva of the diaphania green-wing juba portulacea into the double-layer paper groove for pupation.
The leaf is prepared by spreading old leaves of the chickenaf on a layer and placing the aged larvae in a glass culture dish by using sterilized forceps, and covering a layer of old leaves of the chickenaf with the old leaves of the chickenaf for pupation.
The soil is prepared by mixing clay and sandy soil in a certain proportion with water, stirring, holding into a mass, and placing into a disposable plastic cup. Placing the aged larva into sterilized forceps, covering with gauze, and sealing with rubber band for pupation.
The state of the aged larvae is observed and recorded every day, the pupation time, the number of malformations, deaths and successful pupations are recorded, the weight, the sex ratio and the eclosion rate of the pupation are recorded after the pupation, and the test is set for 3 times of repetition.
3. Test results
By comparing pupation conditions of the Anemophilus furnacalis in three different pupation places of soil, a white paper groove and leaves, the mortality rate, the aberration rate and the pre-pupation duration of the Anemophilus furnacalis in the white paper groove are observed to be lower than those of the other pupation places; the pupation weight of the yellow-fin webworm is higher than that of other pupation places; the ratio of male to female is close to 1: 1. The results are shown in Table 1.
TABLE 1 influence of different pupation sites on pupation of Ostrinia furnacalis
Note: data in the same column indicate (mean ± sem), and data in different lower case letters indicate significant differences (TUKEY method, P < 0.05).
4. Conclusion
By comparing the pupation conditions of the three different transformation places of the old-aged green-wing eulotalis nubilalis larvae, it can be seen that the pupation in the white paper groove not only has short used period, higher pupation rate and heavier pupation weight, but also has the highest pupation rate of the three. In addition, the success rate of pupation of the sesamia anguillata in the leaves is relatively high, because the place is the most common pupation place in natural population, but compared with pupation in a paper groove, the method uses special materials, leaves need to be picked additionally, and in addition, the pupation in the leaves has low space utilization rate and large occupied area, and the procedure is relatively complicated when pupating, so that the manual work is increased. The pupation method in the paper groove is characterized in that 20 mature larvae can be accommodated in the paper groove made of 16 pieces of paper for pupation, and when pupation is taken, only two sides of the paper groove are pulled to slightly pull outwards to tear the cocoons, so that the pupae can be taken out by using sterilized tweezers. Therefore, the method of pupating with a paper groove is more suitable for indoor mass propagation of the Diaphania viridis.
Claims (2)
1. An indoor breeding method of diaphania sinica (Royle) Sesamina, which is characterized by comprising the breeding steps of larvae, pupae, adults and eggs, in particular,
the specific process from initial incubation to preputial is as follows:
spreading the chickpea leaves at the bottom of the larva breeding device, taking out the chickpea leaves fully hatched in the first hatching larva from the hatching device and covering the chickpea leaves on the larva breeding device, then moving the larva breeding device to an artificial climate box for breeding,
changing foodstuff once a day, when changing foodstuff on the next day, installing the separation net in the middle of the larva feeding device, flatly laying fresh foodstuff on the separation net for feeding, taking out the separation net on the third day, cleaning the bottom of the larva feeding device, laying the insects and the foodstuff on the separation net into the bottom of the larva feeding device, flatly laying the fresh foodstuff on the separation net, and repeating the steps until the color of the larva turns red to be in a pre-pupa state; the larva breeding device comprises a box body I (6), a box cover I (5) and a separation net (8), wherein the box cover I (5) covers the top of the box body I (6), the separation net (8) is movably arranged in the middle of the box body I (6), and the mesh size of the separation net (8) is 6-8 mm;
the specific process of pupation is as follows:
picking the larvae in a pre-pupation state from the larva feeding device to a paper groove of a pupation device, moving the pupation device to an artificial climate box, spinning the larvae to be conjugated with the paper groove to make cocoons and pupate, taking out the paper groove after the larvae in the pupation device pupate, pulling the two sides, slightly pulling the two ends to tear the cocoons, and taking out the pupae by using sterilized tweezers; the pupation device comprises a box body II (10), a box cover II (9) and a paper groove (11), wherein the box cover II (9) covers the top of the box body II (10), the paper groove (11) is placed in the box body II (10), and the paper groove (11) is double-layer;
the specific process of eclosion is as follows:
distinguishing male and female pupae under a stereoscope, respectively placing the male and female pupae into two different sterilized eclosion devices, placing the pupae on moisture-preserving filter paper, and moving the eclosion devices into an artificial climate box until eclosion occurs; the eclosion device is characterized in that a base I (16) and a cup body (14) are packaged, the cup body (14) is buckled on the base I (16) in an inverted mode, a through hole is formed in the top surface of the cup body (14), the through hole is sealed by a gauze (15), the mesh size of the gauze (15) is 10-12 meshes, and a cotton ball (13) stained with sugar water is arranged above the through hole; the middle part of the first base (16) is sunken downwards, and moisturizing filter paper (4) is laid on the first base (16);
the specific process from mating to spawning is as follows:
transferring the female and male worms which are just eclosion on the same day into a mating and spawning device, putting the sugarcoated leaves into the copulation and spawning device, transferring the copulation and spawning device into an artificial climate box, allowing the copulation and spawning device to freely mate, taking out the male worms when egg granules appear in the copulation and spawning device, and then taking out and replacing parts with worm eggs on the copulation and spawning device every day until spawning is finished; the mating and spawning device comprises a second base (19), a cup body cylinder (18) and a cup cover (17), wherein the cup body cylinder (18) is arranged on the second base (19), the cup cover (17) covers the cup body cylinder (18), the top surface of the cup cover (17) is provided with a through hole, the through hole is sealed by a gauze (15), the mesh size of the gauze (15) is 10-12 meshes, a cotton ball (13) stained with sugar water is arranged above the through hole, the middle part of the second base (19) is sunken downwards, and moisturizing filter paper (4) is paved on the second base (19);
the specific process from hatching to initial hatching of larvae is as follows:
placing eggs of Sesamia viridis Sesami in an incubator with moisture keeping filter paper at the bottom, spreading tender chicle leaves on the incubator, placing in a climatic chamber until the eggs hatch into larvae,
after the larvae are hatched, the larvae are circularly subcultured according to the steps.
2. The indoor raising method of diaphania citri francis according to claim 1, characterized in that the temperature in the artificial climate box is 25 ℃ ± 1 ℃, and the photoperiod is 14L: 10D, humidity 70% +/-10%.
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