CN111264479A - Indoor breeding device and method for spodoptera frugiperda - Google Patents
Indoor breeding device and method for spodoptera frugiperda Download PDFInfo
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- CN111264479A CN111264479A CN202010279282.9A CN202010279282A CN111264479A CN 111264479 A CN111264479 A CN 111264479A CN 202010279282 A CN202010279282 A CN 202010279282A CN 111264479 A CN111264479 A CN 111264479A
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Abstract
An indoor breeding device and a breeding method for spodoptera frugiperda relate to insect breeding. The invention comprises a larva feeding pipe and an adult feeder, wherein the larva feeding pipe comprises a finger-shaped pipe, filter paper sheets and a gauze, the filter paper sheets are circular and are horizontally placed in the finger-shaped pipe, and the gauze is tightly tied at the pipe orifice of the finger-shaped pipe by a rubber band; the adult feeder comprises an upper water cup, a lower water cup and absorbent cotton balls, wherein the lower water cup is a right-side transparent plastic cup, the upper water cup is an inverted transparent plastic cup, and the lower water cup is oppositely connected with an opening of the upper water cup; the absorbent cotton balls are placed in the lower water cup, a major arc-shaped opening is cut at the upper bottom of the lower water cup, the major arc angle is larger than 270 degrees and smaller than 330 degrees, and a transparent adhesive tape is adhered to the back of the major arc sheet; the pipe orifice of the finger-shaped pipe is inserted into the opening and connected. According to the biological habits and characteristics mastered in the process of researching Spodoptera frugiperda in a laboratory, the invention forms a set of indoor Spodoptera frugiperda feeding method, simplifies the operation steps, saves the time and effectively improves the survival rate of the Spodoptera frugiperda.
Description
Technical Field
The invention relates to insect breeding, in particular to an indoor breeding device and a breeding method for spodoptera frugiperda, which are used for breeding spodoptera frugiperda to obtain data related to biological characteristics of insects.
Background
The plant growth regulator can be used for controlling the growth of spodoptera frugiperda,Spodoptera frugiperdabelongs to the lepidoptera noctuidae family, and is a pest with extremely strong migratory flight capability. The insect is native to the tropical and subtropical regions of the americas, was first discovered in africa in 2016 (1) and rapidly spread to approximately 44 countries in south of sahara, causing severe yield loss in corn. Move into india in 2018 in 5 months, and further spread to southeast asian countries such as srilanka, bangla, thailand, burma, etc. 1 month in 2019, Spodoptera frugiperda was found for the first time in Jiangcheng county of Yunnan province of China. By 10.8 days in 2019, Spodoptera frugiperda has invaded 1538 counties (districts and cities) in 26 provinces (districts and cities) of the country. The spodoptera frugiperda has a wide host range, and the larvae of the spodoptera frugiperda can damage 353 plants in 76 families such as corn, rice, wheat and soybean, and the like, and the spodoptera frugiperda has a great influence on the food production safety in China since the spodoptera frugiperda invades China.
Indoor feeding is an important means for obtaining data related to biological characteristics of the spodoptera frugiperda, and can provide an important theoretical basis for prediction, prediction and comprehensive prevention and control in fields, and because spodoptera frugiperda is a newly-invaded species, no professional spodoptera frugiperda feeding method exists at present, and only the feeding technologies of other species of the spodoptera frugiperda family can be referred to temporarily, but in the present, the feeding technologies have the following defects:
1) the method of breeding the larvae still adopts a fingered tube breeding method, but the 1 st larvae of Spodoptera frugiperda easily die or escape accidentally during the larva transferring process due to the aggregation habit of the larvae; the number of raised spodoptera frugiperda larvae is reduced sharply due to the factors that the high-age larvae are easy to kill each other and eat the low-age larvae;
2) the adult breeding still uses the self-made iron wire insect cage, and is wrapped by the preservative film, and the preservative film is cut to obtain egg grains after the adult lays eggs on the preservative film; the method is very time-consuming and troublesome to operate, and the imagoes of the spodoptera frugiperda have extremely strong flight capability, so that the imagoes are easy to escape by taking the plane in the egg block taking process.
Taking the invention patent 'a prodenia litura indoor artificial propagation method' applied in 2014 as an example, the method adopted for breeding the prodenia litura in the larval stage is as follows: the prodenia litura larvae are placed in a larva breeding box in an artificial climate chamber, and the length, the width and the height of the larva breeding box are 30cm, 20cm and 30cm respectively; the bottom of the insect breeding box needs to be paved with a sand soil layer which is 3-5cm thick and is sieved by a 10-mesh sieve and subjected to ultraviolet sterilization, 200-500 insects are bred in each box, and the box is tightly covered by a 160-mesh gauze, but the method is not suitable for breeding and propagating the Spodoptera frugiperda in large quantities for the following reasons:
1) the number of larvae in the insect-raising box described in the patent is too large, spodoptera frugiperda is very easy to bite each other under the condition, so that most of the larvae are killed by the same kind of larvae or eaten;
2) the insect-breeding box is covered by the gauze, which wastes time and labor, the whole box opening is covered by the gauze, so that the probability that the Spodoptera frugiperda bites the gauze to escape is increased, and the phenomenon is observed in the breeding process;
3) the ultraviolet irradiation does not completely kill the microorganisms in the sandy soil layer.
Disclosure of Invention
The invention aims to solve the problems that the existing method is not suitable for indoor breeding of spodoptera frugiperda and can not accurately obtain the habit of spodoptera frugiperda, and provides an indoor breeding device and a breeding method of spodoptera frugiperda, which are used for breeding spodoptera frugiperda to obtain data related to the biological characteristics of insects.
The indoor feeding device for the Spodoptera frugiperda is characterized by comprising a larva feeding pipe and an adult feeder, wherein the larva feeding pipe comprises a finger-shaped pipe, filter paper sheets and a gauze, the filter paper sheets are circular and are horizontally placed inside the finger-shaped pipe, and the gauze is tightly tied to the pipe orifice of the finger-shaped pipe by a rubber band; the adult feeder comprises an upper water cup, a lower water cup and absorbent cotton balls, wherein the lower water cup is a positive transparent plastic cup with an upward opening, the upper water cup is an inverted transparent plastic cup with a downward opening, the lower water cup is oppositely connected with the opening of the upper water cup and is fixedly connected by a transparent adhesive tape; the absorbent cotton balls are placed in the lower water cup, a major arc-shaped opening is cut at the upper bottom of the lower water cup, the major arc angle is larger than 270 degrees and smaller than 330 degrees, and a transparent adhesive tape is adhered to the back of the major arc sheet; the pipe orifice of the finger-shaped pipe is inserted into the opening and connected.
The wall of the upper water cup and the wall of the lower water cup are provided with a plurality of holes in a staggered manner, and the holes are formed by pricking holes with the needles of the injection needles, so that the adult feeder is convenient to ventilate.
The breeding method of Spodoptera frugiperda comprises two types of independent breeding and population amplification breeding, and specifically comprises the following steps:
1) and independent breeding: the breeding device is used for breeding independently, and the breeding process is as follows:
A. incubation of worm eggs: putting filter paper soaked by distilled water into a culture dish for moisturizing, putting eggs laid by the same adult in the same day into the culture dish, sealing the opening of the culture dish by using a preservative film, ventilating by using a needle prick hole of an insect on the preservative film, recording the laying date and the laying amount of the eggs on the side wall of the culture dish by using a marker pen, and putting the culture dish into a climatic incubator; adding host tender leaves into the culture dish after the egg grains turn black for 1-1.5 days; after 0.5-1 day, the larvae are hatched, the hatching amount is observed and recorded, and the hatching rate is calculated; feeding the primarily hatched larvae on host tender leaves of a culture dish for 12 hours, and then transferring the primarily hatched larvae into a larva feeding pipe for independent feeding to ensure the survival rate of the primarily hatched larvae;
B. larva breeding: putting round filter paper sheets at the bottom of the larva feeding pipe horizontally to facilitate the cleaning of larva excreta in the pipe, wrapping the newly hatched larva with host plants, putting the newly hatched larva into a finger-shaped pipe for single-end feeding, sealing the pipe orifice with gauze, fastening with rubber band, and then putting into an artificial climate incubator; observing and recording the age and the weight of the larvae every day, simultaneously replacing fresh host plants, removing excrement and rotten leaves, cleaning the fresh host plants with clean water and controlling the surface moisture before feeding; pupating the larva after 12-14 days;
C. and (3) pupal stage: stopping adding host plant after pupation of larva, and allowing emergence for 8-9 days; recording the pupation period and calculating the pupation rate in the period;
D. eclosion: after eclosion of the adult, transferring the adult into an adult feeder according to the male-female ratio of 1:1 for pairing and oviposition; identifying the sex of the adult according to the patterns of the front wings of the adult, and calculating the life span, the eclosion rate and the male-female ratio of the adult;
E. adult breeding: after adults in the larva feeding pipe emerge, judging the males and females, putting the females and males into an adult feeder according to the ratio of 1:1, opening the major arc sheet at the bottom of the upper water cup, aligning the finger-shaped pipe opening to the opening, slowly drawing out the gauze, enabling the adults to automatically fly into the upper water cup and the lower water cup, drawing out the finger pipe, rapidly re-sticking the major arc sheet and the bottom of the upper water cup by using a transparent adhesive tape, and recording information required by an experiment on the outer side of the cup wall; after adult insects are paired, supplementing moisture to cotton balls every day, regularly checking the egg laying condition of female insects, taking a new adult feeder after egg laying, opening the major arc pieces at the tops of the two feeders, rightly placing the original adult feeder, inverting the new adult feeder to the upper part, fixing the opened holes in opposite directions, beating the cup bottom at the lower end of the adult feeder below, and flying the adult Spodoptera frugiperda into the new feeder above to continuously mate and lay eggs; after all the adults fly into the new adult feeder, closing the major arc sheet of the new adult feeder, sealing the major arc sheet by using a transparent adhesive tape, shearing off egg masses on the cup wall of the original adult feeder by using scissors, and putting the eggs into a culture dish for continuous incubation;
2) and population amplification propagation:
A. incubation of worm eggs: putting filter paper soaked in distilled water into a transparent plastic box for moisturizing, putting insect eggs into the box, covering a box cover, penetrating air through insect pinprick holes in the box cover, recording date and egg laying amount on the outer wall of the transparent plastic box by using a marking pen, and putting the transparent plastic box into a climatic incubator; adding host tender leaves into the transparent plastic box when the egg grains turn black; after the larvae are hatched out, observing and recording hatching amount, and calculating the hatching rate;
B. larva breeding: opening a 10cm × 5cm opening in the middle of the cover of the transparent plastic box, covering a gauze, adhering the gauze to the edge of the opening by using hot melt adhesive, keeping 16-24 larvae in each transparent plastic box, replacing fresh host tender leaves every day, cleaning the fresh host tender leaves with clear water, drying the leaves in the air before feeding, and removing excrement and rotten leaves;
C. and (3) pupal stage: taking fine sand, carrying out high-pressure sterilization at 120 ℃ for 15 minutes, putting the fine sand into an oven, drying the fine sand for 6 hours at 65 ℃, and cooling the fine sand for later use; taking a transparent plastic box, uniformly spreading a layer of sterilized fine sand with the thickness of 1cm on the bottom of the box, putting 4-5 days of larvae into the box for pupation, wherein the pre-pupation period is 1-3 days, and no plant is added in the pupation period; taking out pupa when the color of pupa becomes dark brown, putting six pupae into an adult feeder according to the proportion of three female pupae and three male pupae, and sealing;
D. eclosion: after the male and female pupae eclosion, sucking 10% honey water with an injector, piercing the outer wall of the adult feeder, injecting the honey water into the degreased cotton ball inside to ensure that the honey water is soaked in the degreased cotton ball for feeding adults;
E. pairing adults: when the adults are paired, supplementing moisture to cotton balls every day, checking the egg laying condition of female insects, replacing an adult feeder when egg grains appear on the cup wall, taking a new adult feeder after egg laying, opening the major arc sheets at the tops of the two feeders, placing the original adult feeder right under, inverting the new adult feeder to the upper part, fixing the open holes in opposite directions, beating the cup bottom at the lower end of the adult feeder below, and flying the adult Spodoptera frugiperda into the new feeder above to continuously mate and lay eggs because the adult Spodoptera frugiperda has the upward flying habit; and after all the adults fly into the new adult feeder, closing the major arc sheet of the new adult feeder, sealing the major arc sheet by using a transparent adhesive tape, shearing off egg masses on the cup wall of the original adult feeder by using scissors, and putting the eggs into a culture dish for continuous incubation.
The illumination period L of the artificial climate incubator is 16h to 8h, the temperature is 27 +/-1 ℃, and the humidity is 70 +/-5%.
The host plant is selected from tender leaves of corn, tender leaves of wheat or fresh corn kernels, the leaves of the corn are all tender leaves before the large bell mouth, and only the tender leaves are tender leaves between the large bell mouth and the heading stage; the wheat plant height is 20 + -2 cm and is tender leaf.
The gauze is 150 meshes.
According to the indoor feeding device and the feeding method for spodoptera frugiperda, the biological habits and the characteristics of different insect states of spodoptera frugiperda are mastered in the process of researching the spodoptera frugiperda in a laboratory, the original feeding method for spodoptera frugiperda is greatly improved, a set of indoor feeding method for spodoptera frugiperda is formed, the operation steps are greatly simplified, a large amount of time is saved, the survival rate of the spodoptera frugiperda is effectively improved, and the method has the following specific advantages:
1. the invention provides a feeding device and a matching method suitable for the spodoptera frugiperda adults, which are convenient for pairing, nutrition supplement and timely transfer of egg masses, greatly improve the working efficiency and improve the hatching rate of egg granules;
2. the invention provides a breeding method which is beneficial to pupation of the aged larvae, and the survival rate of the aged larvae and the eclosion rate of pupae are improved;
3. the situation that 1 st larva dies and escapes in large quantity, and old larva eats low-age larva or larvae of the same age to eat each other is avoided;
4. different feeding methods are adopted according to different feeding purposes (mass feeding for population expansion breeding or biological habit observation), which is beneficial to stabilizing the indoor population quantity;
5. the materials used in the invention are simple and easily available, and the price is low, so that the feeding cost can be greatly reduced.
Drawings
FIG. 1 is a schematic view of the structure of the adult rearing device of the present invention.
Wherein, go up drinking cup 1, lower drinking cup 2, major arc piece 3, finger pipe 4.
Detailed Description
Example 1: an indoor raising device for spodoptera frugiperda is characterized by comprising a larva raising pipe and an adult raising device, wherein the larva raising pipe comprises a finger-shaped pipe 4, filter paper sheets and a gauze with 150-mesh specification, the filter paper sheets are circular and are horizontally placed inside the finger-shaped pipe 4, and the gauze is tied tightly at the pipe orifice of the finger-shaped pipe 4 by a rubber band; the adult feeder comprises an upper water cup 1, a lower water cup 2 and absorbent cotton balls, wherein the lower water cup 2 is a positive transparent plastic cup with an upward opening, the upper water cup 1 is an inverted transparent plastic cup with a downward opening, the lower water cup 2 is oppositely connected with the opening of the upper water cup 1 and is fixedly connected by using a transparent adhesive tape; the absorbent cotton ball is placed in the lower water cup 2, a major arc-shaped opening is cut at the upper bottom of the lower water cup 2, the major arc angle is larger than 270 degrees and smaller than 330 degrees, and a transparent adhesive tape is adhered to the back of the major arc sheet 3; the pipe orifice of the finger-shaped pipe 4 is inserted into the opening and connected.
The wall of the upper water cup 1 and the wall of the lower water cup 2 are alternately provided with a plurality of holes which are formed by pricking holes with the needles of the injection needles, so that the adult feeder is convenient to ventilate.
The breeding method of Spodoptera frugiperda comprises two types of independent breeding and population amplification breeding, and specifically comprises the following steps:
1) and independent breeding: the breeding device is used for breeding independently, and the breeding process is as follows:
A. incubation of worm eggs: putting filter paper soaked by distilled water into a culture dish for moisturizing, putting eggs laid by the same adult in the same day into the culture dish, sealing the opening of the culture dish by using a preservative film, ventilating by using insect needle prick holes on the preservative film, recording the laying date and the laying egg amount by using a marker pen on the side wall of the culture dish, and putting the culture dish into a phytotron, wherein the phytotron is an MG (-300A) type culture box produced by Shanghai-Hengscientific instruments, Ltd; adding host tender leaves into the culture dish after the egg grains turn black for 1-1.5 days; after 0.5-1 day, the larvae are hatched, the hatching amount is observed and recorded, and the hatching rate is calculated; feeding the primarily hatched larvae on host tender leaves of a culture dish for 12 hours, and then transferring the primarily hatched larvae into a larva feeding pipe for independent feeding to ensure the survival rate of the primarily hatched larvae;
B. larva breeding: the bottom of the larva breeding pipe is horizontally provided with a circular filter paper sheet for facilitating the cleaning of the excrement of the larva in the pipe, the initially hatched larva is wrapped by a host plant and is placed into the finger-shaped pipe 4 for single-end breeding, the pipe orifice is sealed by a gauze and is fastened by a rubber band, and then the pipe is placed into an artificial climate incubator; observing and recording the age and the weight of the larvae every day, simultaneously replacing fresh host plants, removing excrement and rotten leaves, cleaning the fresh host plants with clean water and controlling the surface moisture before feeding; pupating the larva after 12-14 days;
C. and (3) pupal stage: stopping adding host plant after pupation of larva, and allowing emergence for 8-9 days; recording the pupation period and calculating the pupation rate in the period;
D. eclosion: after eclosion of the adult, transferring the adult into an adult feeder according to the male-female ratio of 1:1 for pairing and oviposition; identifying the sex of the adult according to the patterns of the front wings of the adult, and calculating the life span, the eclosion rate and the male-female ratio of the adult;
E. adult breeding: after adults in the larva feeding pipe emerge, judging the males and females, putting the females and males into an adult feeder according to the ratio of 1:1, opening the major arc sheet 3 at the bottom of the upper water cup 1, aligning the opening of the finger-shaped pipe 4 to the opening, slowly drawing out the gauze, enabling the adults to fly into the upper water cup 1 and the lower water cup 2 by themselves, drawing out the finger-shaped pipe, quickly re-sticking the major arc sheet 3 and the bottom of the upper water cup 1 by using a transparent adhesive tape, and recording information required by an experiment outside of the cup wall; after adult pairing, supplementing moisture to cotton balls every day, regularly checking the egg laying condition of female insects, taking a new adult feeder after egg laying, opening the major arc pieces 3 at the tops of the two feeders, rightly placing the original adult feeder, inverting the new adult feeder to the upper part, fixing the open holes in opposite directions, beating the cup bottom at the lower end of the adult feeder below, and flying the adult Spodoptera frugiperda into the new feeder above to continuously mate and lay eggs; after all the adults fly into the new adult feeder, closing the major arc sheet 3 of the new adult feeder, sealing the minor arc sheet with a transparent adhesive tape, shearing off egg masses on the cup wall of the original adult feeder with scissors, and placing the eggs into a culture dish for continuous incubation;
2) and population amplification propagation:
A. incubation of worm eggs: putting filter paper soaked in distilled water into a transparent plastic box for moisturizing, putting insect eggs into the box, covering a box cover, penetrating air through insect pinprick holes in the box cover, recording date and egg laying amount on the outer wall of the transparent plastic box by using a marking pen, and putting the transparent plastic box into a climatic incubator; adding host tender leaves into the transparent plastic box when the egg grains turn black; after the larvae are hatched out, observing and recording hatching amount, and calculating the hatching rate;
B. larva breeding: opening a 10cm × 5cm opening in the middle of the cover of the transparent plastic box, covering a 150-mesh gauze, adhering the gauze to the edge of the opening with hot melt adhesive, keeping 16-24 larvae in each transparent plastic box, replacing fresh host tender leaves every day, cleaning the fresh host tender leaves with clear water, drying in the air, and removing feces and rotten leaves;
C. and (3) pupal stage: taking fine sand, carrying out high-pressure sterilization at 120 ℃ for 15 minutes, putting the fine sand into an oven, drying the fine sand for 6 hours at 65 ℃, and cooling the fine sand for later use; taking a transparent plastic box, uniformly spreading a layer of sterilized fine sand with the thickness of 1cm on the bottom of the box, putting 4-5 days of larvae into the box for pupation, wherein the pre-pupation period is 1-3 days, and no plant is added in the pupation period; taking out pupa when the color of pupa becomes dark brown, putting six pupae into an adult feeder according to the proportion of three female pupae and three male pupae, and sealing;
D. eclosion: after the male and female pupae eclosion, sucking 10% honey water with an injector, piercing the outer wall of the adult feeder, injecting the honey water into the degreased cotton ball inside to ensure that the honey water is soaked in the degreased cotton ball for feeding adults;
E. pairing adults: when the adults are paired, supplementing moisture to cotton balls every day, checking the egg laying condition of female insects, replacing an adult feeder when egg grains appear on the cup wall, taking a new adult feeder after egg laying, opening the major arc sheets 3 at the tops of the two feeders, placing the original adult feeder right under, inverting the new adult feeder to the upper part, fixing the open holes in opposite directions, beating the cup bottom at the lower end of the adult feeder below, and flying the adults into the new feeder above due to the upward flying habit of the Spodoptera frugiperda adults, and continuing mating and egg laying; after all the adults fly into the new adult feeder, the major arc sheet 3 of the new adult feeder is closed and sealed by using the transparent adhesive tape, and then the egg mass on the cup wall of the original adult feeder is cut off by using scissors and placed in a culture dish for continuous incubation.
In the whole culture period, the illumination period L: D of the artificial climate incubator is 16h:8h, the temperature is 27 +/-1 ℃, and the humidity is 70 +/-5%.
The host plant is selected from tender leaves of corn, tender leaves of wheat or fresh corn kernels, wherein the leaves of the corn are tender leaves before the large bell mouth, and only the tender leaves are tender leaves between the large bell mouth and the heading stage; the wheat plant height is 20 + -2 cm and is tender leaf.
The gauze is 150 meshes.
Claims (6)
1. An indoor feeding device for Spodoptera frugiperda is characterized by comprising a larva feeding pipe and an adult feeder, wherein the larva feeding pipe comprises a finger-shaped pipe (4), filter paper sheets and a gauze, the filter paper sheets are circular and horizontally placed inside the finger-shaped pipe (4), and the gauze is tightly tied at the pipe orifice of the finger-shaped pipe (4) by a rubber band; the adult feeder comprises an upper water cup (1), a lower water cup (2) and absorbent cotton balls, wherein the lower water cup (2) is a positive transparent plastic cup with an upward opening, the upper water cup (1) is an inverted transparent plastic cup with a downward opening, the lower water cup (2) is oppositely connected with the opening of the upper water cup (1) and is fixedly connected by a transparent adhesive tape; the absorbent cotton balls are placed in the lower water cup (2), a major arc-shaped opening is cut at the upper bottom of the lower water cup (2), the major arc angle is larger than 270 degrees and smaller than 330 degrees, and a transparent adhesive tape is adhered to the back of the major arc piece (3); the pipe orifice of the finger-shaped pipe (4) is inserted into the opening hole for connection.
2. The indoor feeding device of Spodoptera frugiperda as claimed in claim 1, wherein the wall of the upper water cup (1) and the lower water cup (2) are provided with a plurality of holes in a staggered manner, and the holes are formed by pricking with needles of injection needles.
3. A method for breeding Spodoptera frugiperda is characterized by comprising the following steps of individual breeding and population expansion breeding:
1) and independent breeding: the breeding device is used for breeding independently, and the breeding process is as follows:
A. incubation of worm eggs: putting filter paper soaked by distilled water into a culture dish for moisturizing, putting eggs laid by the same adult in the same day into the culture dish, sealing the opening of the culture dish by using a preservative film, ventilating by using a needle prick hole of an insect on the preservative film, recording the laying date and the laying amount of the eggs on the side wall of the culture dish by using a marker pen, and putting the culture dish into a climatic incubator; adding host tender leaves into the culture dish after the egg grains turn black for 1-1.5 days; after 0.5-1 day, the larvae are hatched, the hatching amount is observed and recorded, and the hatching rate is calculated; feeding the primarily hatched larvae on host tender leaves of a culture dish for 12 hours, and then transferring the primarily hatched larvae into a larva feeding pipe for independent feeding to ensure the survival rate of the primarily hatched larvae;
B. larva breeding: the bottom of the larva feeding pipe is horizontally provided with a circular filter paper sheet for facilitating the cleaning of the excrement of the larva in the pipe, the initially hatched larva is wrapped by a host plant and is placed into a finger-shaped pipe (4) for single-end feeding, the pipe orifice is sealed by a gauze and is tightly tied by a rubber band, and then the pipe is placed into an artificial climate incubator; observing and recording the age and the weight of the larvae every day, simultaneously replacing fresh host plants, removing excrement and rotten leaves, cleaning the fresh host plants with clean water and controlling the surface moisture before feeding; pupating the larva after 12-14 days;
C. and (3) pupal stage: stopping adding host plant after pupation of larva, and allowing emergence for 8-9 days; recording the pupation period and calculating the pupation rate in the period;
D. eclosion: after eclosion of the adult, transferring the adult into an adult feeder according to the male-female ratio of 1:1 for pairing and oviposition; identifying the sex of the adult according to the patterns of the front wings of the adult, and calculating the life span, the eclosion rate and the male-female ratio of the adult;
E. adult breeding: after adults in the larva feeding pipe emerge, judging the males and females, putting the females and males into an adult feeder according to the ratio of 1:1, opening the major arc sheet (3) at the bottom of the upper water cup (1), aligning the opening of the finger-shaped pipe (4) to the opening, slowly drawing out the gauze, enabling the adults to fly into the upper water cup (1) and the lower water cup (2) by themselves, drawing out the finger-shaped pipe, quickly sticking the major arc sheet (3) and the bottom of the upper water cup (1) again by using a transparent adhesive tape, and recording information required by an experiment on the outer side of the cup wall; after adult pairing, supplementing moisture to cotton balls every day, regularly checking the egg laying condition of female insects, taking a new adult feeder after egg laying, opening the superior arc sheets (3) at the tops of the two feeders, rightly placing the original adult feeder, inverting the new adult feeder to the upper side, fixing the open holes in opposite directions, beating the cup bottom at the lower end of the adult feeder below, and enabling the adult Spodoptera frugiperda to fly into the new feeder above due to the upward flying habit of the adult Spodoptera frugiperda to continuously mate and lay eggs; after all the adults fly into the new adult feeder, closing the excellent arc sheet (3) of the new adult feeder, sealing the excellent arc sheet by using a transparent adhesive tape, shearing off egg masses on the cup wall of the original adult feeder by using scissors, and putting the egg masses into a culture dish for continuous incubation;
2) and population amplification propagation:
A. incubation of worm eggs: putting filter paper soaked in distilled water into a transparent plastic box for moisturizing, putting insect eggs into the box, covering a box cover, penetrating air through insect pinprick holes in the box cover, recording date and egg laying amount on the outer wall of the transparent plastic box by using a marking pen, and putting the transparent plastic box into a climatic incubator; adding host tender leaves into the transparent plastic box when the egg grains turn black; after the larvae are hatched out, observing and recording hatching amount, and calculating the hatching rate;
B. larva breeding: opening a 10cm × 5cm opening in the middle of the cover of the transparent plastic box, covering a gauze, adhering the gauze to the edge of the opening by using hot melt adhesive, keeping 16-24 larvae in each transparent plastic box, replacing fresh host tender leaves every day, cleaning the fresh host tender leaves with clear water, drying the leaves in the air before feeding, and removing excrement and rotten leaves;
C. and (3) pupal stage: taking fine sand, carrying out high-pressure sterilization at 120 ℃ for 15 minutes, putting the fine sand into an oven, drying the fine sand for 6 hours at 65 ℃, and cooling the fine sand for later use; taking a transparent plastic box, uniformly spreading a layer of sterilized fine sand with the thickness of 1cm on the bottom of the box, putting 4-5 days of larvae into the box for pupation, wherein the pre-pupation period is 1-3 days, and no plant is added in the pupation period; taking out pupa when the color of pupa becomes dark brown, putting six pupae into an adult feeder according to the proportion of three female pupae and three male pupae, and sealing;
D. eclosion: after the male and female pupae eclosion, sucking 10% honey water with an injector, piercing the outer wall of the adult feeder, injecting the honey water into the degreased cotton ball inside to ensure that the honey water is soaked in the degreased cotton ball for feeding adults;
E. pairing adults: when the adults are paired, supplementing water to cotton balls every day, checking the egg laying condition of female insects, when egg grains appear on the cup wall, replacing an adult feeder, taking a new adult feeder after egg laying, opening the superior arc sheets (3) at the tops of the two feeders, rightly placing the original adult feeder below, inverting the new adult feeder above, fixing the opened holes opposite to the opened holes, beating the cup bottom at the lower end of the adult feeder below, and enabling the adults to fly into the new feeder above due to the fact that the Spodoptera frugiperda adults have upward flying habits, and continuing mating and egg laying; and after all the adults fly into the new adult feeder, closing the excellent arc sheet (3) of the new adult feeder, sealing the excellent arc sheet by using a transparent adhesive tape, shearing off egg masses on the cup wall of the original adult feeder by using scissors, and putting the egg masses into a culture dish for continuous incubation.
4. The method for raising Spodoptera frugiperda as claimed in claim 3, wherein the illumination period L: D: 16h:8h, the temperature is 27 ± 1 ℃, and the humidity is 70 ± 5%.
5. The method for raising Spodoptera frugiperda as claimed in claim 3, wherein the host plant is selected from young leaves of corn, young leaves of wheat or fresh corn kernels, the leaves of corn are young leaves before the large bell mouth, and only the new leaves are young leaves between the large bell mouth and heading stage; the wheat plant height is 20 + -2 cm and is tender leaf.
6. An indoor feeding device for Spodoptera frugiperda as claimed in claim 1, wherein the gauze is 150 mesh.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111758672A (en) * | 2020-07-16 | 2020-10-13 | 中国热带农业科学院环境与植物保护研究所 | Group breeding method for feeding spodoptera frugiperda larvae with young corn shoots |
| CN111758673A (en) * | 2020-07-16 | 2020-10-13 | 中国热带农业科学院环境与植物保护研究所 | Method for expanding propagation of spodoptera frugiperda trichogramma by preserving spodoptera frugiperda egg masses |
| CN112205358A (en) * | 2020-09-28 | 2021-01-12 | 云南农业大学 | Indoor population propagation method for spodoptera frugiperda |
| CN114403098A (en) * | 2021-12-22 | 2022-04-29 | 中国农业科学院农业基因组研究所 | Lotus herb direct chest flea beetle pupating device and pupating method thereof |
| CN116982681A (en) * | 2023-08-04 | 2023-11-03 | 烟台大学 | Spodoptera frugiperda artificial feed and preparation method thereof |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111758672A (en) * | 2020-07-16 | 2020-10-13 | 中国热带农业科学院环境与植物保护研究所 | Group breeding method for feeding spodoptera frugiperda larvae with young corn shoots |
| CN111758673A (en) * | 2020-07-16 | 2020-10-13 | 中国热带农业科学院环境与植物保护研究所 | Method for expanding propagation of spodoptera frugiperda trichogramma by preserving spodoptera frugiperda egg masses |
| CN111758673B (en) * | 2020-07-16 | 2021-11-23 | 中国热带农业科学院环境与植物保护研究所 | Method for expanding propagation of spodoptera frugiperda trichogramma by preserving spodoptera frugiperda egg masses |
| CN111758672B (en) * | 2020-07-16 | 2022-02-01 | 中国热带农业科学院环境与植物保护研究所 | Group breeding method for feeding spodoptera frugiperda larvae with young corn shoots |
| CN112205358A (en) * | 2020-09-28 | 2021-01-12 | 云南农业大学 | Indoor population propagation method for spodoptera frugiperda |
| CN114403098A (en) * | 2021-12-22 | 2022-04-29 | 中国农业科学院农业基因组研究所 | Lotus herb direct chest flea beetle pupating device and pupating method thereof |
| CN116982681A (en) * | 2023-08-04 | 2023-11-03 | 烟台大学 | Spodoptera frugiperda artificial feed and preparation method thereof |
| CN116982681B (en) * | 2023-08-04 | 2024-02-06 | 烟台大学 | Spodoptera frugiperda artificial feed and preparation method thereof |
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