KR19990079066A - Swallowtail butterfly breeding method - Google Patents

Swallowtail butterfly breeding method Download PDF

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KR19990079066A
KR19990079066A KR1019980011456A KR19980011456A KR19990079066A KR 19990079066 A KR19990079066 A KR 19990079066A KR 1019980011456 A KR1019980011456 A KR 1019980011456A KR 19980011456 A KR19980011456 A KR 19980011456A KR 19990079066 A KR19990079066 A KR 19990079066A
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mating
breeding
spawning
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lux
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KR100271852B1 (en
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설광열
이희권
홍성진
김남정
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김강권
대한민국(관리부서:농촌진흥청)
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/90Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
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Abstract

본 발명은 동양특산종인 호랑나비(Papilio xuthus)를 실내 또는 온실에서의 교미 및 산란법을 개발하여 실내 대량증식법을 체계화한 것이다.The present invention is to develop a mating and spawning method of the Oriental Swallowtail butterfly (Papilio xuthus) indoors or in the greenhouse to systemize the indoor mass growth method.

교미는 실내에서 우화후 2-3일째 설탕물을 먹여 안정시킨 다음 인공교배(Hand mating)시키거나 형광등 3.400lux 조도 아래에 기주식물을 놓고 암수비율 1 : 1로 방사하여 자연교미시킨다.The mating is stabilized by feeding sugar water 2-3 days after allegory indoors, then artificial mating (Hand mating) or placing the host plant under fluorescent light 3.400lux illuminance and sprinkling with male and female ratio 1: 1.

산란은 실내에서 형광등 3,400 lux와 700 lux 조도사이에 기주식물이나 기주식물의 잎분말 또는 메탄올 추출물을 바른 여지를 놓고 설탕물을 계속공급하면서 산란을 유도하거나 온실의 자연광하에서 기주식물에 산란을 유도한다.Scattering induces spawning by continuously supplying sugar water with room to apply host plant or leaf powder or methanol extract between 3,400 lux and 700 lux illuminance of fluorescent lamp indoors or inducing spawning of host plant under natural light in greenhouse .

산란된 알은 1일 이내에 채란하여 뚜껑이 있는 용기에 넣어 25℃에서 잘 보호하고 3일후 부화된 유충을 기주 또는 인공먹이로 사육한다.Scattered eggs are fertilized within 1 day and placed in a container with a lid, and then well protected at 25 ° C., and after 3 days, hatched larvae are raised by host or artificial feeding.

Description

호랑나비의실내 대량증식법Swallowtail butterfly breeding method

본 발명은 동양특산종인 호랑나비를 실내에서 대량증식시키는 방법에 관한 것이다. 보다 상세하게는 호랑나비의 교미, 산란법을 체계화하여 실내에서 효과적으로 교미 및 산란시킴으로써 호랑나비를 연중 대량증식이 가능하도록 하여 사라져가는 곤충자원을 보존하는데 목적을 두고 있다.The present invention relates to a method of mass-proliferating indoor swallowtail butterfly larvae. More specifically, it aims to preserve the disappearing insect resources by systematically mating and spawning the swallowtail butterfly, effectively mating and spawning it indoors to enable mass breeding of the swallowtail butterfly throughout the year.

본 발명과 관련된 종래기술은 없으며 인공교배(hand mating)법이 있으나 산란법이 확립되지 않아 대량증식에 한계가 있다.There is no prior art related to the present invention and there is artificial mating (hand mating) method, but the scattering method is not established, there is a limit to mass growth.

그러나 본 발명은 실내에서 호랑나비의 교미방법과 산란방법을 개발하여 종래 대량증식의 문제점을 해결하였다.However, the present invention has developed a mating method and spawning method of swallowtail butterfly indoors to solve the problem of conventional mass breeding.

본 발명은 호랑나비의 증식에 있어서 광생리를 이용하여 실내 또는 온실에서 인공교배와 자연교배로 교미시켜 실내의 형광등하에서 방사하여 산란을 유도하거나 온실의 자연광하에서 방사하여 산란시킨 다음 채란하여 용기내에서 부화시켜 유충을 사육한 후 용화된 번데기의 정상우화를 유도시켜 대량증식하는 방법을 제공하고자 한다.In the present invention, in the growth of the swallowtail butterfly, mating by artificial mating and natural mating in the indoor or greenhouse using light physiology to induce the scattering by emitting under the fluorescent lamp in the room or by scattering by scattering in the natural light of the greenhouse and then hatched in the container After the breeding of larvae to induce normal allegory of the pupae, and to provide a method of mass growth.

도 1 은 호랑나비의 실내 대량증식법에 대한 체계도이다.1 is a schematic diagram of the indoor mass multiplication method of the swallowtail butterfly.

교미는 실내에서 우화후 2-3일째 설탕물을 먹여 안정시킨 다음 인공교배시키거나 형광등 3.400lux 조도아래에 기주식물을 놓고 암수비율 1 : 1로 방사하여 자연교미를 유도한다.The mating is stabilized by feeding sugar water 2-3 days after allegory indoors, then artificially mating or placing host plants under fluorescent light 3.400lux illuminance to induce natural mating by radiating male and female ratio 1: 1.

산란은 실내에서 3,400 lux 와 700 lux 조도사이에 기주식물이나 기주식물의 잎분말 또는 메탄올 추출물을 바른 여지를 놓고 설탕물을 계속 공급하면서 산란을 유도하거나, 온실의 자연광하에서 기주식물에 산란을 유도한다.Spawning induces spawning by continuously supplying sugar water in the room with 3,400 lux and 700 lux illuminance, leaving room with leaf powder or methanol extract of host plant, or spawning host plant under natural light in greenhouse. .

유충사육은 산란된 알은 1일 이내에 채란하여 뚜껑이 있는 용기에 넣어 25℃에서 보호하고 3일후 부화된 유충을 기주식물 또는 인공먹이로 사육한다.Larvae eggs are laid within 1 day and placed in a container with a lid and protected at 25 ° C. After 3 days, the larvae are reared with host plants or artificial feed.

우화는 유충사육후 용화된 번데기를 수집하여 용화후 7-8일째 번데기를 본드 등으로 철망에 부착시켜 정상우화를 유도한다.The allegory collects the pupae after larval rearing and attaches the pupa 7 to 8 days after the larvae to bond to the wire mesh to induce normal allegory.

[실시예 1] 교미Example 1 Mating

인공교배는 실내에서 우화 2-3일째Artificial mating takes place 2-3 days indoors

설탕물을 먹여 안정시킨 다음 30분 - 1 시간후 두꺼운 책갈피에 암컷의 날개를 접어서 고정하고 핀셋으로 수컷의 파악기를 벌려 암컷의 배 끝부분에 수컷의 등판끝 마디 갈고리를 넣으면 20-30초후에 교미가 이루어져 얌전해지며 결합된 암수를 기주식물에 붙여 주어 평균 40-60분 동안 교미시킨다.After 30 minutes-1 hour of stabilization with sugar water, the female's wings are folded and fixed in a thick bookmark, and the male's abdomen is opened at the end of the female's belly with tweezers. It is made calm and puts the combined male and female to host plants for mating for an average of 40 to 60 minutes.

자연교배는 실내에서 우화 2-3일째 설탕물을 먹인 다음 형광등 3,400 lux하에 기주식물을 놓고 암수비율을 1 : 1로 투입, 설탕물을 계속 공급하면서 자연교미를 유도시킨다.Natural breeding feeds sugar water indoors 2-3 days in fable, then places host plants under 3,400 lux of fluorescent lamp, and puts male and female ratio at 1: 1.

[실시예 2] 산란Example 2 Scattering

실내 산란은 교미한 암컷을 형광등 아래 3,400 lux-700 lux 조도 사이에 방사하여 기주식물이나 기주식물의 잎분말 또는 메탄올 추출물을 바른 여지에 산란토록 유도한다. 온실산란은 온실의 자연광에 기주식물을 놓고 교미시킨 암컷을 방사하여 산란시킨다.Indoor spawning releases mated females between 3,400 lux and 700 lux illuminances under fluorescent lamps to induce spawning of host plants, leaf powder of host plants or methanol extract. Greenhouse spawning involves spawning females mated with host plants in the natural light of the greenhouse.

[실험예 1] 호랑나비의 조건별 교미율, 산란수 및 부화율 확인시험.Experimental Example 1 Test of mating rate, spawning number and hatching rate of the swallowtail butterfly.

실내 및 온실에서 형광등, 백열등, 자연광별로 암수비율에 따른 교미율, 산란수,부화율을 측정하였던 바, 표 1과 같이 나타났고 온실의 자연광하에서도 교미율은 낮았으나 산란수는 많았고 부화율도 높았다.The mating rate, scattering number, and hatching rate according to the male and female ratio of fluorescent lamps, incandescent lamps, and natural light were measured as shown in Table 1.

한편 사육실내에서는 일반형광등하에서 암수비율 1 : 1일 때 교미율 및 부화율이 높았다.On the other hand, the mating and hatching rates were high at male to female ratios of 1: 1 under normal fluorescent lamps.

호랑나비의 조건별 교미율, 산란수 및 부화율 확인시험 결과Test results of mating rate, spawning number and hatching rate of the swallowtail butterfly 구분division 광원Light source 공간space 암수비율Male and female ratio 교미율(%)Mating rate (%) 산란수/마리Spawning / mari 부화율Hatching rate 실내inside 일반형광등General fluorescent light 큰케이지Big cage 2:12: 1 5.65.6 231231 57.757.7 *1:1* 1: 1 40.040.0 277277 56.956.9 1:21: 2 26.726.7 315315 31.531.5 작은케이지Small cage 2:12: 1 00 00 00 1:11: 1 00 130130 00 1:21: 2 00 4242 00 got빛형광등got fluorescent 큰케이지Big cage 2:12: 1 5.65.6 140140 21.421.4 1:11: 1 13.313.3 190190 28.628.6 1:21: 2 5.65.6 194194 21.121.1 작은케이지Small cage 2:12: 1 00 192192 39.039.0 1:11: 1 13.313.3 212212 42.942.9 1:21: 2 00 8181 00 백열등Incandescent light 큰케이지Big cage 2:12: 1 00 3939 00 1:11: 1 6.76.7 152152 37.537.5 1:21: 2 6.76.7 145145 32.432.4 작은케이지Small cage 2:12: 1 00 1717 00 1:11: 1 00 5252 00 1:21: 2 6.76.7 163163 20.920.9 일반형광등General fluorescent light 사육실Breeding room *1:1* 1: 1 45.545.5 427.9427.9 68.068.0 온실greenhouse 자연광Natural light 2:12: 1 5.65.6 382382 59.859.8 1:11: 1 26.726.7 432432 67.067.0 1:21: 2 20.020.0 397397 63.363.3 실내inside (인공교배)(Artificial cross) 사육실Breeding room 1:11: 1 100100 391.7391.7 68.468.4

* 큰케이지 : 120×150×70cm, 작은케이지 : 50×60×40cm* Large Cage: 120 × 150 × 70cm, Small Cage: 50 × 60 × 40cm

사육실 : 430×260×240cm 온실 : 700×500×300cm Hand matingBreeding room: 430 × 260 × 240cm Greenhouse: 700 × 500 × 300cm Hand mating

[실험예 2] 형광등으로부터 조도별 교미용 및 산란수 확인실험Experimental Example 2 Experiment for Confirming Coagulation and Scattering Number by Illuminance from Fluorescent Lamps

사육실( 430×260×240cm)에서 기주식물을 상, 중, 하 높이별로 놓고 10%설탕용액을 공급하면서 일반형광등의 조도에 따라 교미율과 산란수를 측정한 바, 표 2와 같이 나타났고 조도가 높은 상층부(3,400 lux)에서 교미율이 높았고 산란 역시 700 lux이상의 상층부에 중점적으로 산란하는 경향이었다.In the breeding room (430 × 260 × 240cm), host plants were placed at the top, middle, and bottom heights, and 10% sugar solution was supplied, and the mating rate and the number of scattered eggs were measured according to the illuminance of general fluorescent lamps. The copulation rate was high at the upper layer (3,400 lux) and scattering also tended to focus on the upper layer above 700 lux.

조도별 교미율 및 산란수 확인 실험결과Experimental results of mating rate and scattering number by roughness 조도 (lux)Roughness (lux) 교미율(%)Mating rate (%) 산란수(개)/마리Spawning water () 3,400 (상)3,400 (upper) 36.436.4 149.7149.7 700 (중)700 (medium) 9.19.1 192.1192.1 280 (하)280 (bottom) 00 86.186.1

[실험예 3]Experimental Example 3

설탕용액의 농도에 따른 호랑나비의 수명 및 산란수확인실험Life Test and Scattering Water Test of Swallowtail Butterfly According to the Concentration of Sugar Solution

사육실 (430×260×240cm)에서 인공교배시킨 암컷에 설탕물을 공급하면서 그 수명 및 산란수를 조사한 결과 표 3과 같이 나타났고 10% 이상의 농도의 설탕물을 공급했을 경우 수명이 길어지고 산란수도 많았다.As a result of examining the lifespan and spawning water while supplying sugar water to females artificially bred in the breeding room (430 × 260 × 240cm), the lifespan was longer and the number of spawning water was longer when the sugar water of 10% or more was supplied. .

설탕용액 농도별 수명 및 산란수 확인실험 결과Experimental Results of Life and Scattering Water by Sugar Solution Concentration 농도 (%)Concentration (%) 수명(일)Lifetime (days) 산란수(마리당)Spawning water (per head) 대조구(물)Control (water) 3.3±0.743.3 ± 0.74 65.865.8 1010 13.8±1.3413.8 ± 1.34 387.2387.2 2020 14.8±1.6714.8 ± 1.67 411.9411.9

[실험예 4]Experimental Example 4

호랑나비의 산란선호성Swallowtail butterfly's spawn preference

상자(120×150×70cm)내에서 인공교배시킨 암컷을 10%설탕용액을 공급하면서 그 산란선호성을 조사한 결과 표 4와 같이 나타났고 기주식물이 아닌 곳(대조구)에서 거의 산란하지 않았으나 기주식물의 잎분말을 도말한 여지에 상당량을 산란하였고 기주식물의 추출물은 그 농도가 높을수록 산란수가 많아졌다.In the box (120 × 150 × 70cm), the females artificially cross-bred with 10% sugar solution, and their scattering preferences were shown in Table 4.They showed little scattering in non-host plants (control) but A large amount of egg was scattered in the leaves where the leaf powder was smeared.

호랑나비의 산란선호성Swallowtail butterfly's spawn preference 구분division 대조구Control 기주식물Host plants 기주잎분말(1g)Host Leaf Powder (1g) 기주식물의 메탄올추출물(g)Methanol extract of host plant (g) 0.40.4 0.80.8 1.61.6 총산란수Total scattering 55 387387 152152 00 6.16.1 117117 마리당평균Average 1.71.7 129129 50.750.7 00 20.320.3 39.039.0

[실시예 3] 호랑나비의 실내 대량증식법Example 3 Indoor Mass Propagation Method of Swallowtail Butterfly

기주식물(탱자 또는 산초나무)또는 인공사료를 이용하여 23℃, 65%RH. 광주 16L : 8D(휴면용 : 20℃, 65%, 12L:2D)로 조절한 실내에서 2-3일에 한번씩 먹이를 교체하면서 유충을 사육하고 용화후 2일째 번데기를 수집한다.23 ° C, 65% RH using host plants (tanza or vinegar) or artificial feed. Gwangju 16L: 8D (sleeping: 20 ℃, 65%, 12L: 2D) in the room controlled by changing the food every 2-3 days, breeding larvae and pupa 2 days after gestation.

수집된 번데기를 샤레에 넣어 보호한 후 7-8일째 몸이 투명해지면서 날개형성이 보이기 시작하면 본드를 이용해 철망등에 부착시켜 우화시키며, 휴면용 용화후 약 20일이 경과할 때 4℃에서 60일간 두었다가 다시 23℃로 옮겨 우화시킨다. 우화한 성충들을 케이지내에서 설탕물을 공급해 주며 보호한 후 2-3일째 인공먹이를 먹여 실내에서 인공교배 또는 자연교배시키고, 온실 또는 실내에서 산란을 유도한다.After protecting the collected pupa in the saree, after 7-8 days, the body becomes transparent and wing formation begins to appear, attach it to a wire mesh using a bond and allegorize it. Leave for a day, then transfer to 23 ° C for allegory. The adult females are fed with sugar water in the cage and protected by artificial feeding 2-3 days after the artificial feeding or indoor breeding, and induces spawning in the greenhouse or indoors.

알은 산란1일 이내에 채란하여 뚜껑이 있는 용기에 잘 보호하고 3일후에 부화된 유충을 기주 또는인공사료에 옮겨준다.Eggs are egg-layed within 1 day of laying, well protected in lidded containers, and after 3 days the hatched larvae are transferred to host or man-made feed.

본 발명은 호랑나비에 설탕물을 먹인후 인공교배 또는 형광등 3,40O LUX이하 조도하에서 기주식물위에 암수비율 1 : 1로 방사하여 자연교미를 유도시켜 실내에서 3,400 - 700 lux 조도하 또는 자연광에서 산란을 유도하여 용기에서 유충을 사육하고 정상우화를 유도하여 연중 호랑나비를 대량증식시킬 수 있다.The present invention, after feeding sugar water to the tiger butterfly, artificial radiation or fluorescent lamp under the illuminance of 3,40O LUX or less in the male and female ratio 1: 1 on the host plants to induce natural mating and scattering in the room under 3,400-700 lux illuminance or natural light Induce the larvae in the container to induce a normal allegory can increase the year-round swallowtail butterfly.

특히, 암수비율 1 : 1에서 교미율 40%, 산란수 200 이상, 부화율 57% 등 높은 증식효과를 제공하여 곤충자원의 자연학습재료 및 펫트곤충상품화를 촉진시킬 수 있다.In particular, it can promote the commercialization of natural learning materials and pet insects of insect resources by providing a high proliferation effect such as a male and female ratio of 1: 1, a mating rate of 40%, a spawning water of 200 or more, and a hatching rate of 57%.

Claims (5)

우화후 2-3일째인 호랑나비성충을 형광등 아래서 방사하여 자연교배시키는 교배단계와, 형광등 아래서 설탕물을 먹이로 공급하며 기주식물 또는 그 대체물에 산란을 유도하는 산란단계와, 산란 1일이내에 채란하여 기주식물 또는 인공먹이로 사육하는 부화사육단계와, 용화후 7-8일 지난 번데기를 철망 등에 부착하여 우화를 유도하는 우화단계로 이루어짐을 특징으로 하는 호랑나비의 실내 대량증식 방법A mating step of spontaneous mating by radiating a swallowtail butterfly, which is 2-3 days after allegory, under a fluorescent lamp, a laying step of feeding sugar water under a fluorescent lamp to induce spawning of host plants or its substitutes, and egg production within 1 day of laying. Indoor breeding method of swallowtail butterfly characterized in that it consists of a hatching breeding step of breeding with host plants or artificial feed, and an allegory step of inducing allegory by attaching chrysalis past 7-8 days after yonghwa 제 1 항에 있어서,The method of claim 1, 교배단계는 인공교배 또는 암수 1 : 1의 비율로 자연교배시킴을 특징으로 하는 호랑나비의 실내 대량증식 방법The breeding stage is a method of mass breeding indoors of swallowtail butterflies, characterized by natural breeding by artificial breeding or male to female ratio. 제 1 항에 있어서,The method of claim 1, 교배단계의 형광등 조도는 3,400 lux인 것을 특징으로 하는 호랑나비의 실내 대량증식 방법Fluorescent light intensity of the mating stage is 3,400 lux 제 1 항에 있어서,The method of claim 1, 산란단계의 형광등 조도는 3,400 - 700 lux인 것을 특징으로 하는 호랑나비의 실내 대량증식방법.Fluorescent light intensity in the scattering stage is 3,400-700 lux indoor mass growth method of the swallowtail butterfly. 제 1 항에 있어서,The method of claim 1, 산란단계의 기주식물의 대체물은 기주식물의 잎분말 또는 그 메탄올 추출물을 도포한 여과지임을 특징으로 하는 호랑나비의 실내 대량증식 방법Substituents of host plants in the spawning stage are the leaf powder of host plants or filter paper coated with methanol extract thereof.
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