CN109122597A - A kind of method for breeding of the small beetle of beehive - Google Patents
A kind of method for breeding of the small beetle of beehive Download PDFInfo
- Publication number
- CN109122597A CN109122597A CN201811246046.6A CN201811246046A CN109122597A CN 109122597 A CN109122597 A CN 109122597A CN 201811246046 A CN201811246046 A CN 201811246046A CN 109122597 A CN109122597 A CN 109122597A
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- China
- Prior art keywords
- beehive
- beetle
- small
- breeding
- larva
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000254173 Coleoptera Species 0.000 title claims abstract description 47
- 238000000034 method Methods 0.000 title claims abstract description 18
- 238000009395 breeding Methods 0.000 title claims abstract description 17
- 230000001488 breeding effect Effects 0.000 title claims abstract description 17
- 241000238631 Hexapoda Species 0.000 claims abstract description 16
- 238000010010 raising Methods 0.000 claims abstract description 12
- 239000004576 sand Substances 0.000 claims abstract description 12
- 235000012907 honey Nutrition 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 241000382353 Pupa Species 0.000 claims abstract description 7
- 230000012447 hatching Effects 0.000 claims abstract description 4
- 239000002023 wood Substances 0.000 claims description 15
- 229910001220 stainless steel Inorganic materials 0.000 claims description 12
- 239000010935 stainless steel Substances 0.000 claims description 12
- 239000011521 glass Substances 0.000 claims description 9
- 229920003229 poly(methyl methacrylate) Polymers 0.000 claims description 4
- 239000004926 polymethyl methacrylate Substances 0.000 claims description 4
- 230000000384 rearing effect Effects 0.000 claims description 3
- 241000167854 Bourreria succulenta Species 0.000 claims 1
- 239000002994 raw material Substances 0.000 abstract description 3
- 235000013601 eggs Nutrition 0.000 description 12
- 241000256844 Apis mellifera Species 0.000 description 5
- 102000002322 Egg Proteins Human genes 0.000 description 4
- 108010000912 Egg Proteins Proteins 0.000 description 4
- 210000004681 ovum Anatomy 0.000 description 4
- 239000004033 plastic Substances 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 230000001418 larval effect Effects 0.000 description 2
- 230000017448 oviposition Effects 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 241000220276 Aethina tumida Species 0.000 description 1
- 108010034145 Helminth Proteins Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 229920000715 Mucilage Polymers 0.000 description 1
- 241000220274 Nitidulidae Species 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000006399 behavior Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 230000003031 feeding effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 244000000013 helminth Species 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of method for breeding of the small beetle of beehive.It is raised the following steps are included: the small adult beetle of beehive is put into insect cage, timing feeds man-made feeds and water, after sexal maturity, egg apparatus will be adopted and be put into insect cage, next day takes out, it is put into raising in the larva raising box equipped with man-made feeds, egg hatching is at larva, until growing to mature larva, mature larva is put into wet sand, mature larva, which pierces in wet sand, pupates, and pupa is sprouted wings into adult；The man-made feeds include pollen and honey, and the mass ratio of the pollen and honey is 1:1.The method for breeding of the small beetle of beehive of the invention, raw material sources are extensive, and low in cost, practicability is extremely strong；It is not only simple to operation, and standardized breeding technology more standardizes, and readily available age in days is consistent, meets and tests required amount of small beetle of beehive.
Description
Technical field:
The invention belongs to the small beetles of beehive to raise field, and in particular to a kind of method for breeding of the small beetle of beehive.
Background technique:
The small beetle of beehive, scientific name: Aethina tumida Murray, 1867 belong to coleoptera, Nitidulidae, world animal
Health organization (OIE) is classified as one of big important pathogen body of honeybee six, is the international quarantine pest for destroying honeybee industry.Bee
The small beetle of case belongs to holometabolous insect, including ovum phase, larval phase, pupa time and adult stage.One small beetle of beehive from ovum to
Adult, about between 38~81 days, the specific time changes with the variation of the factors such as ambient temperature, soil moisture.It is closing
Under suitable environment, the small beetle of beehive can breed for 6 generations every year.
The small beetle larva of beehive is food with honey and pollen, they dig a hole across lair, and institute is destroyed entirely through place, makes bee
Honey is off color often, and with fermentation phenomenon, also gives out a kind of peculiar smell similar to rotten orange.It is broken in lair and capping
It is bad, and in the case where fermentation, honey can blister and overflow lair, or even outflow beehive.In addition, the small beetle larva institute of beehive is through it
Place can leave a kind of mucilage with stink, and this substance can force honeybee to abandon nest and escape.In order to preferably carry out the small first of beehive
Worm scientific research needs the small beetle of a certain number of beehives, therefore, it is non-to establish the laboratory labor standardization raising small beetle of beehive
Want necessary and urgent.
Summary of the invention:
The object of the present invention is to provide a kind of raisings of small beetle of beehive easy to operate, safe and practical, standardization raising
Method.
The method for breeding of the small beetle of beehive of the invention, comprising the following steps: the small adult beetle of beehive is put into insect cage
Middle raising, periodically feeds man-made feeds and water, after sexal maturity, will adopt egg apparatus and is put into insect cage, and next day takes out, and is put into and is equipped with
Raising in the larva raising box of man-made feeds, egg hatching is at larva, until growing to mature larva, mature larva is put into wet sand
In, mature larva, which pierces in wet sand, pupates, and pupa is sprouted wings into adult；The man-made feeds include pollen and honey, the flower
The mass ratio of powder and honey is 1:1.
The small adult beetle of the beehive and the rearing conditions of larva are both preferably 32 DEG C of temperature, and relative humidity 80% hides
Light.
The water content of the wet sand is preferably 10%.
It is preferred that the coverslip for adopting egg apparatus and including two pieces of glass slides He being fixed among two pieces of glass slides.
It is preferred that the insect cage includes cuboid wood frame, the top and bottom of the cuboid wood frame
It is respectively equipped with poly (methyl methacrylate) plate and plank, four side of cuboid wood frame is equipped with to be matched not with side frame
Become rusty steel gauze, and the stainless steel yarn of the cuboid wood frame one side is equipped with the tubular yarn of operation port and both ends open on the net
Cloth, it is online that the stainless steel yarn at operation port is fixed in one end of the Tubular shape gauze.
The gauze is preferably the stainless steel gauze of 120 mesh.
It is preferred that the bottom of the cuboid wood frame is equipped with support leg.
The method for breeding of the small beetle of beehive of the invention, the method for establishing the small beetle of laboratory artificial feeding, convenient for obtaining
Age in days is consistent, meets and tests required amount of small beetle of beehive.
Advantage of the present invention is conducive to carry out indoor each in the small beetle of laboratory rearing beehive, standardized breeding technology foundation
Item research work.
Primary raw material pollen and honey of the present invention, raw material sources are extensive, and low in cost, practicability is extremely strong；Secondly, this method
It is not only simple to operation, and standardized breeding technology more standardizes.
Detailed description of the invention:
Fig. 1 is the structural schematic diagram of insect cage of the invention；
Fig. 2 is the side view for adopting egg apparatus of the invention；
Fig. 3 is the worm state of the small beetle different times of beehive；Wherein a: the ovum phase of the small beetle of beehive；B: the pupa of the small beetle of beehive
Phase；C: the larval phase of the small beetle of beehive；D: the adult stage of the small beetle of beehive；
Fig. 4 is the feeding effect figure of the small beetle of beehive；Wherein a: the sexal maturity male and female mating of the small beetle of beehive；B: beehive is small
Beetle adopts egg apparatus；C: the small beetle larva of beehive and its man-made feeds；D: the small beetle of the beehive pupated in sandy soil.
Appended drawing reference meaning in figure: 1, cuboid wood frame；2, poly (methyl methacrylate) plate；3, plank；4, stainless steel gauze；5,
Operation port；6, Tubular shape gauze；7, support leg；8, glass slide；9, coverslip.
Specific embodiment:
The following examples are further illustrations of the invention, rather than limiting the invention.
The preparation of man-made feeds in the embodiment of the present invention: pollen and honey are uniformly mixed in mass ratio for 1:1, are placed on
Storage in 0 DEG C of refrigerator.
Embodiment 1:
As shown in Figure 1, insect cage includes cuboid wood frame 1 (length × width × height is about 30cm × 30cm × 30cm),
The top of cuboid wood frame 1 is equipped with poly (methyl methacrylate) plate 2, and the bottom of cuboid wood frame 1 is equipped with plank 3, cuboid wood
The bottom of matter frame 1 is additionally provided with support leg 7 (high 3cm)；1 four side of cuboid wood frame is equipped with to be matched with side frame
120 mesh stainless steel gauze 4, facilitate observation the small beetle of beehive behavior, the stainless steel yarn of 1 one side of cuboid wood frame
Net 4 is equipped with the Tubular shape gauze 6 of operation port 5 (orifice diameter 35cm) and both ends open, and Tubular shape gauze 6 is tubular black nylon
Gauze, one end of Tubular shape gauze 6 are fixed on the stainless steel gauze 4 at operation port 5, are conducive to adopt worm, are placed feed, adopt egg apparatus
Deng channel, Tubular shape gauze 6 is knotted when not used, prevents adult from escaping from outside insect cage.Every cage insect population is suitble to establish
300-1000 or so.
By 100g man-made feeds, water-feed and just 500 small adult beetles of beehive (female male imago ratio be 1:1) for sprouting wings are put
It is placed in insect cage, the water-feed 125mm culture dish of adult, and it is equally big to put in ware perimeter in one piece of perimeter and culture dish
Small clean pollution-free sponge pours into after water not only allow worm to drink water but also can prevent from falling into water and be drowned.Insect cage is placed in manually
Weather incubator is raised under 32 DEG C of temperature, relative humidity 80%, dark conditions, and water and man-made feeds change once every 3d.It is female
Male imago 3-7d after emergence starts to mate, and female adult starts largely to lay eggs, and female adult starts that egg apparatus (such as Fig. 2 will be adopted when oviposition
It is shown, adopt the coverslip 9 that egg apparatus includes two pieces of glass slides 8 and is fixed among two pieces of glass slides 8；Add among two glass slides
Enter coverslip, adhered with glass cement, be prepared and adopt egg apparatus, lay eggs for the small beetle female adult of beehive) it is put into insect cage, next day takes
(after taking out after 12h, a female adult stays overnight general 100 of egg laying amount) out, is put into the larva raising box equipped with 20g man-made feeds
It is interior, larva raising box is placed in insect cage, is raised under 32 DEG C of temperature, relative humidity 80%, dark conditions, egg hatching at
Larva (survival rate 100%), until grow to mature larva, larva enter after ageing stage (meet 15-20d after ovum) will be from
It is climbed out of in larva raising box.The mature larva climbed out of is put into the wet sand equipped with 10cm depth, and (water content of wet sand is 10%, and sand makes
With the 2h that sterilizes under 120 DEG C of high temperature is required before, to kill helminth) plastic storage case (length × width × height be 42cm ×
26cm × 15cm) in, plastic storage case mouth is sealed with gauze and is tightened with rubber band, is trained after preventing pupa from sprouting wings in artificial climate
It supports and flies around in case, plastic storage case is put into artificial climate incubator, in 32 DEG C of temperature, relative humidity 80%, shading
Under the conditions of raise, mature larva, which pierces in wet sand, pupates, pupa sprout wings at adult (Fig. 3 and 4).
Claims (7)
1. a kind of method for breeding of the small beetle of beehive, which comprises the following steps: be put into the small adult beetle of beehive feeding
It is raised in worm cage, periodically feeds man-made feeds and water, after sexal maturity, egg apparatus will adopted and be put into insect cage, next day takes out, and is put into
It is raised in larva raising box equipped with man-made feeds, egg hatching is at larva, until growing to mature larva, mature larva is put into
In wet sand, mature larva, which pierces in wet sand, pupates, and pupa is sprouted wings into adult；The man-made feeds include pollen and honey, described
Pollen and honey mass ratio be 1:1.
2. the method for breeding of the small beetle of beehive according to claim 1, which is characterized in that the small adult beetle of the beehive
Rearing conditions with larva are 32 DEG C of temperature, relative humidity 80%, shading.
3. the method for breeding of the small beetle of beehive according to claim 1 or 2, which is characterized in that the wet sand it is aqueous
Amount is 10%.
4. the method for breeding of the small beetle of beehive according to claim 1, which is characterized in that the egg apparatus of adopting includes two pieces
Glass slide and the coverslip being fixed among two pieces of glass slides.
5. the method for breeding of the small beetle of beehive according to claim 1, which is characterized in that the insect cage includes rectangular
Body wood frame is respectively equipped with poly (methyl methacrylate) plate and plank, the length at the top and bottom of the cuboid wood frame
Four side of cube wood frame is equipped with the stainless steel gauze matched with side frame, the cuboid wood frame side
The stainless steel yarn in face is equipped with the Tubular shape gauze of operation port and both ends open on the net, and operation is fixed in one end of the Tubular shape gauze
Stainless steel yarn at mouthful is online.
6. the method for breeding of the small beetle of beehive according to claim 5, which is characterized in that the stainless steel gauze is
The stainless steel gauze of 120 mesh.
7. the method for breeding of the small beetle of beehive according to claim 5 or 6, which is characterized in that the cuboid is wooden
The bottom of frame is equipped with support leg.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811246046.6A CN109122597A (en) | 2018-10-23 | 2018-10-23 | A kind of method for breeding of the small beetle of beehive |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811246046.6A CN109122597A (en) | 2018-10-23 | 2018-10-23 | A kind of method for breeding of the small beetle of beehive |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109122597A true CN109122597A (en) | 2019-01-04 |
Family
ID=64809872
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811246046.6A Pending CN109122597A (en) | 2018-10-23 | 2018-10-23 | A kind of method for breeding of the small beetle of beehive |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109122597A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109984093A (en) * | 2019-03-29 | 2019-07-09 | 西南大学 | A kind of Groton bug rearging cage and Groton bug method for breeding |
| CN110115318A (en) * | 2019-04-09 | 2019-08-13 | 上海师范大学 | A kind of versatility beetle jelly and preparation method thereof |
| CN111480621A (en) * | 2020-06-10 | 2020-08-04 | 中南大学 | Method for collecting and feeding cadaveric beetles in laboratory |
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| US20090126258A1 (en) * | 2007-11-16 | 2009-05-21 | Kennedy Anthony Joseph | Insect trap |
| US8066988B1 (en) * | 2004-12-29 | 2011-11-29 | The United States Of America As Represented By The Secretary Of Agriculture | In-hive trap and lure for control of the small hive beetle, Aethina tumida |
| CN202857585U (en) * | 2012-09-05 | 2013-04-10 | 东莞市农业科学研究中心 | Insect feeding cage used for feeding citrus fruit flies |
| AU2015238870A1 (en) * | 2015-10-08 | 2017-04-27 | Bruce Quentin Wallace | A Small Hive Beetle Trap And Method Of Use |
| CN107517934A (en) * | 2017-09-06 | 2017-12-29 | 云南省林业科学院 | A kind of more worm state box for feeding pests of citrus fruit fly and its method for breeding |
| CN206866408U (en) * | 2017-05-23 | 2018-01-12 | 中国热带农业科学院环境与植物保护研究所 | A kind of simple breeding apparatus of parasitic wasp for being easy to spawning |
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2018
- 2018-10-23 CN CN201811246046.6A patent/CN109122597A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8066988B1 (en) * | 2004-12-29 | 2011-11-29 | The United States Of America As Represented By The Secretary Of Agriculture | In-hive trap and lure for control of the small hive beetle, Aethina tumida |
| US20090126258A1 (en) * | 2007-11-16 | 2009-05-21 | Kennedy Anthony Joseph | Insect trap |
| CN202857585U (en) * | 2012-09-05 | 2013-04-10 | 东莞市农业科学研究中心 | Insect feeding cage used for feeding citrus fruit flies |
| AU2015238870A1 (en) * | 2015-10-08 | 2017-04-27 | Bruce Quentin Wallace | A Small Hive Beetle Trap And Method Of Use |
| CN206866408U (en) * | 2017-05-23 | 2018-01-12 | 中国热带农业科学院环境与植物保护研究所 | A kind of simple breeding apparatus of parasitic wasp for being easy to spawning |
| CN107517934A (en) * | 2017-09-06 | 2017-12-29 | 云南省林业科学院 | A kind of more worm state box for feeding pests of citrus fruit fly and its method for breeding |
Non-Patent Citations (4)
| Title |
|---|
| BRONWEN W. CRIBB ET AL.: "Aiming for the management of the small hive beetle, Aethina tumida, using relative humidity and diatomaceous earth", 《APIDOLOGIE》 * |
| LILIA I DE GUZMAN ET AL.: "Temperature affects Aethina tumida (Coleoptera: Nitidulidae) Development", 《JOURNAL OF APICULTURAL RESEARCH》 * |
| PETER NEUMANN ET AL.: "Laboratory Rearing of Small Hive Beetles Aethina Tumida(Coleoptera, Nitidulidae)", 《JOURNAL OF APICULTURAL RESEARCH》 * |
| THOMAS MURRLE ET AL.: "Mass production of small hive beetles(Aethina tumida, Coleoptera: Nitidulidae)", 《JOURNAL OF APICULTURAL RESEARCH》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109984093A (en) * | 2019-03-29 | 2019-07-09 | 西南大学 | A kind of Groton bug rearging cage and Groton bug method for breeding |
| CN110115318A (en) * | 2019-04-09 | 2019-08-13 | 上海师范大学 | A kind of versatility beetle jelly and preparation method thereof |
| CN111480621A (en) * | 2020-06-10 | 2020-08-04 | 中南大学 | Method for collecting and feeding cadaveric beetles in laboratory |
| CN111480621B (en) * | 2020-06-10 | 2021-06-01 | 中南大学 | Method for collecting and feeding cadaveric beetles in laboratory |
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| PB01 | Publication | ||
| PB01 | Publication | ||
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Application publication date: 20190104 |