CN108012995A - A kind of indoor feeding method and device of green wing thin,tough silk open country snout moth's larva - Google Patents

Abstract

The present invention relates to a feeding method and a feeding device for insects, in particular to an indoor feeding method and a device for the green-winged silk borer, including the feeding of larvae, pupae and adults, and the steps of collecting and hatching eggs, in particular to the green-winged silk borer The newly hatched larvae are raised in a larva rearing device, fed with sugar gum leaves until they are pre-pupated, and then placed in a pupation device until they pupate; after identifying male and female pupae, they are placed separately in eclosion boxes, and paired after eclosion Wait for them to mate and lay eggs in the adult rearing device; collect the eggs in the adult rearing device, put them in a clean round plastic petri dish with moisturizing filter paper on the bottom, spread young sugar gum leaves on top, and place them in an artificial climate box , pay attention to moisturizing every day until it hatches; after the larvae hatch, the larvae are subcultured according to the above-mentioned steps. The invention is for the study of the influence of biological and abiotic factors on the survival and reproduction of the green silk borer population, and provides an insect source with relatively consistent progress of insect state development.

Description

Indoor breeding method and device for the green-winged silk borer

technical field

The invention relates to an insect feeding method and a feeding device thereof, in particular to an indoor feeding method and device of the green silk borer.

Background technique

Diaphania angustalis Snellen (Lepidoptera: Pyralidae) is the main foliar pest of sugar tree Alastonia scalaris L. The degree of its harm is increasing year by year, which has aroused people's attention. The larva spins silk and rolls the leaves vertically to form a bud, and eats the mesophyll inside to cause damage. It has strong concealment and large food intake. In severe cases, the damage rate of plants reaches 90%-95%; the damage rate of young leaves reaches 85%; Withered and yellow, causing fallen leaves, resulting in bare branches and bare poles, seriously reducing its use value, medicinal value, and ornamental value, and affecting the growth of sugar gum and the development of biopharmaceutical industry. Although the green-winged silk borer is widely distributed in my country, the damage is different due to the different temperature and other conditions in different places. Generally, there are 6-7 generations a year, and the damage takes a long time and is difficult to control. The entire leaves of the severely damaged landscaping plants and street trees are eaten up, leaving only dead leaves, which seriously affects the urban landscape, image and ecological benefits.

Studies have shown that the main factors affecting insect population outbreaks are hosts, insect source bases (including overwintering and oversummer bases), and ecological environments that are conducive to population increase (such as lack of effective natural enemies) (Meng Qingfan. Forest plant community diversity and insect pest natural Advances in Control Research. World Forestry Research 2003). First of all, the species richness and abundance of garden ecosystems are low, and the planting of a large area of a single tree species provides abundant food for the pest population to promote its massive reproduction, which is one of the main reasons for its population outbreak. Because in the garden ecosystem, the tree species for greening are limited, and the large-scale planting of a single tree species provides abundant food for pests, which helps the rapid reproduction of pest (especially herbivorous species) populations. Secondly, being able to adapt to the local climatic conditions and forming a certain winter and summer base is the basis for the outbreak of pest populations. It is mainly reflected in the tolerance of the pest population to the local extreme high/low temperature (including the extreme high/low temperature and the duration of the high/low temperature), strong tolerance, high survival rate, and high population base. Finally, due to the low species diversity of the garden ecosystem, it is impossible to form a diversified habitat and rich food sources to accommodate more insect species, natural enemies, predatory arthropods, insectivorous birds and other animal species. It cannot regulate or resist the damage of pests through the species diversity in its own system. Due to the limited tree species selected for urban greening space and artificial greening, severe human disturbance in the city, narrow underground space for tree growth, and plant malnutrition caused by poor management and maintenance, it is difficult to create a garden landscape with high species richness , making it difficult for the urban green space system to establish a stable and balanced ecological function. As a result, it can not attract more abundant natural enemy species, so that the pest population lacks the suppression of effective natural enemies and causes its population to grow explosively. Some scholars have proposed the natural enemy hypothesis (Natural enemy hypothesis) (Meng Qingfan. Forest plant community diversity and natural pest control ability Research Advances. World Forestry Research 2003). Therefore, mastering the influence of main and key biotic (such as hosts, natural enemies) and abiotic factors (temperature, humidity, and light) on the survival and reproduction of the P. chinensis population is the premise and basis for analyzing its population outbreak.

However, to understand the effects of biotic (such as hosts, natural enemies) and abiotic factors (temperature, humidity, and light) on the survival and reproduction of the P. chinensis population requires a large number of indoor populations with relatively consistent morphological development progress. Source of test insects. At present, there is no report on the technology of indoor rearing of the green-winged silk borer both at home and abroad. In view of this, it is particularly important to develop a set of indoor artificial rearing techniques for the green-winged silk borer to achieve long-term and large-scale subculture of test insects, so as to provide sufficient insect sources for exploring its outbreak mechanism.

Contents of the invention

The object of the present invention is to provide a kind of indoor breeding method and device of the green silk borer, the raising method and the device can raise a large number, and the relatively consistent development progress of the green silk borer, as biological (such as host , natural enemies) and abiotic factors (temperature, humidity and light) on the population survival and reproduction of P.

In order to achieve the above object, the technical scheme adopted in the present invention is:

A kind of indoor rearing method of green silk borer, comprising the feeding steps of larvae, pupae, adults and eggs, specifically,

The specific process from newly hatched larvae to prepupa is:

Lay the chicle leaves on the bottom of the larva rearing device, take out the chicle leaves covered with newly hatched larvae from the hatching device and cover them on the gum leaves of the larva rearing device, then move the larva rearing device to an artificial climate box to raise,

Change the food once a day. When changing the food on the second day, install the partition net in the middle of the larva rearing device, and spread fresh food on the partition net for feeding. On the third day, take out the partition net and clean the bottom of the larva rearing device. Feces, and then spread the worms and food materials on the separation net into the bottom of the larva rearing device, and spread fresh food materials on the separation net, repeat the above steps until the larva body color turns red and is in a pre-pupa state.

The specific process of pupation is:

Pick the larvae in the pre-pupation state from the larva rearing device to the paper trough of the pupation device, and move the pupation device to the artificial climate box. The larvae spin silk and bind the paper trough to make cocoons and pupate, and wait for pupation After the larvae in the device pupate, take out the paper trough, pull the two sides, and gently pull towards the two ends to tear the cocoon, and then take out the pupae with sterilized tweezers.

The specific process of feathering is:

The male and female pupae were identified under a stereoscope, and the male and female pupae were placed in two different sterile eclosion devices. The pupae were placed on moisturizing filter paper, and the eclosion device was moved to an artificial climate box until eclosion.

The specific process from mating to spawning is:

Move the females and males that have just emerged at the same age to the mating and oviposition device, and put the sugar gum leaves, and then move the mating and oviposition device to the artificial climate box, and let them mate freely. When the mating and oviposition device appears Take out the male worm when the eggs are in, and then take out and replace the parts of the mating and oviposition device with eggs every day until the end of oviposition;

The specific process from hatching to newly hatched larvae is:

Put the eggs of the green-winged silk moth into the hatching device with moisturizing filter paper on the bottom, spread the young sugar gum leaves on top, and place them in an artificial climate box until the eggs hatch into newly hatched larvae.

After the larvae hatch, the larvae are subcultured according to the above-mentioned steps.

Further, the larva rearing device comprises a box body one (6), a box cover one (5) and a partition net (8), the box cover one (5) covers the top of the box body one (6), and the compartment net (8) The movable one is arranged in the middle part of the box body one (6), and the mesh size of the partition net (8) is 6-8mm.

Further, the pupation device includes box body two (10), box cover two (9) and a paper slot (11), the box cover two (9) is covered on the top of the box body two (10), and the paper slot (11) Put in the box body two (10), the paper groove (11) is double-layer.

Further described feathering device packaging base one (16) and cup body (14), cup body (14) is buckled on the base one (16), and cup body (14) top surface is provided with through hole, and through hole is made of yarn Net (15) is sealed, and gauze (15) mesh size is 10-12 order, and the cotton ball (13) that is stained with sugar water is arranged above the through hole; There are moisturizing filter papers (4).

Further, the mating and spawning device includes a base two (19), a cup body (18) and a cup cover (17), the cup body (18) is arranged on the base two (19), and the cup cover (17) covers On the cup body tube (18), the top surface of the cup cover (17) is provided with a through hole, and the through hole is sealed by a gauze (15). The mesh size of the gauze (15) is 10-12 mesh. The cotton ball (13) of sugar water, the base two (19) middle part is sunken downwards, and the base two (19) is covered with moisturizing filter paper (4).

6. The indoor rearing method of the green leaf borer according to claim 1, characterized in that the temperature in the artificial climate box is 25°C±1°C, the photoperiod is 14L:10D, and the humidity is 70% ±10%.

Outstanding substantive features and remarkable progress of the present invention are:

1. The present invention can provide a comparatively consistent worm source for researching the influence of organisms (such as hosts, natural enemies and pathogens) and abiotic factors (temperature, humidity and light) on the survival and reproduction of the green leaf silkworm population. Moreover, the related feeding device has simple structure, low cost, easy operation, manpower saving and recycling use.

2, adopt circular plastic box among the present invention as the rearing device of silk borer larvae, and there is detachable net in the middle part of the device. Add food on the Internet, and the larvae will actively crawl to fresh food, which can not only reduce the contact with the worms when changing the food, but also isolate the pollution of the excrement to the food, and solve the problem of larvae dying due to contact with pathogenic microorganisms in indoor breeding problems, while saving a lot of manpower.

3, the pupation device among the present invention adopts the double-deck paper tank to supply pre-pupation pupation, is the conjugation blade pupation (pupation rate: 88%; Abnormality rate: 1.5%;) and Pupation in the soil (pupation rate: 68%; deformity rate: 7.2%), this device not only has a higher success rate of pupation, but also a low deformity rate (pupation rate: 92%; deformity rate: 1.1%), and The use area is saved, and each device can accommodate 20 prepupae on average for the pupation process. Moreover, the design of the double-layer paper tank can make the larvae pupate both on the upper and lower sides of the paper tank, and will not affect each other, and will not cause the cocoon chamber to be destroyed and transferred to make cocoons for many times, resulting in physical exertion and failure to pupate. In addition, it also saves manpower and reduces mechanical damage to the body during the process of taking pupae.

4. The eclosion device in the present invention can provide a good eclosion place, so that adults can successfully spread their wings after eclosion, and can supply nutrition in time. The size of the device is suitable, and it is convenient to take the worms, and will not cause the adult worms to escape during the experimental operation. At the same time, it can also be used as a breeding place for short-term adults, which can provide adults of different ages for research on reproductive behavior, and provide data support for the improvement of biological control technology.

5. The mating and oviposition device among the present invention provides a good mating place, and adults can have a higher mating rate of 56% in this device and only 15% in the cage, and can completely collect all ovum grains. The device can be disassembled into three parts, the upper cover, the cup body and the lower cover, which can be freely assembled, so that the entire device can be avoided due to the female laying eggs in a certain part of the device, thereby saving costs. Adults in this device have a long lifespan, with an average lifespan of up to 21 days, while the lifespan of adults living in net cages is only 12 days. Therefore, using the device can obtain females with a longer oviposition period, obtain more eggs, and achieve the purpose of mass reproduction.

Description of drawings

Fig. 1 is a schematic structural view of the larva rearing device of the present invention.

Fig. 2 is a schematic structural view of the pupation device of the present invention.

Fig. 3 is a structural schematic diagram of the adult eclosion device of the present invention.

Fig. 4 is a schematic structural view of the adult mating and oviposition device of the present invention.

Fig. 5 is a schematic structural view of the hatching device of the present invention.

Detailed ways

The technical solution of the present invention will be further elaborated below through examples.

A kind of indoor rearing method of green silk borer, comprising the feeding steps of larvae, pupae, adults and eggs, specifically,

The specific process from newly hatched larvae to prepupa is:

Lay the chicle leaves on the bottom of the larva rearing device, take out the chicle leaves covered with newly hatched larvae from the hatching device and cover them on the gum leaves of the larva rearing device, then move the larva rearing device to an artificial climate box to raise,

Change the food once a day. When changing the food on the second day, install the partition net in the middle of the larva rearing device, and spread fresh food on the partition net for feeding. On the third day, take out the partition net and clean the bottom of the larva rearing device. Feces, and then spread the worms and food materials on the separation net into the bottom of the larva rearing device, and spread fresh food materials on the separation net, repeat the above steps until the larva body color turns red and is in a pre-pupa state.

The specific process of pupation is:

Pick the larvae in the pre-pupation state from the larva rearing device to the paper trough of the pupation device, and move the pupation device to the artificial climate box, and wait for spinning to combine with the paper trough to make cocoons until they pupate, and then pupate. After the larvae in the device pupate, take out the paper trough, pull the two sides, and gently pull towards the two ends to tear the cocoon, and then take out the pupae with sterilized tweezers.

The specific process of feathering is:

The male and female pupae were identified under a stereoscope, and the male and female pupae were placed in two different sterile eclosion devices. The pupae were placed on moisturizing filter paper, and the eclosion device was moved to an artificial climate box until eclosion.

The specific process from mating to spawning is:

Move the females and males that have just emerged at the same age to the mating and oviposition device, and put the sugar gum leaves, and then move the mating and oviposition device to the artificial climate box, and let them mate freely. When the mating and oviposition device appears Take out the male worm when the eggs are in, and then take out and replace the parts of the mating and oviposition device with eggs every day until the end of oviposition;

The specific process from hatching to newly hatched larvae is:

Put the eggs of the green-winged silk moth into the hatching device with moisturizing filter paper on the bottom, spread the young sugar gum leaves on top, and place them in an artificial climate box until the eggs hatch into newly hatched larvae.

After the larvae hatch, the larvae are subcultured according to the above-mentioned steps.

As shown in Figure 1, 1 is a petri dish, 2 is a leaf, 3 is an egg, 4 is a moisturizing filter paper, and 21 is a cover.

As shown in Fig. 2, described larva rearing device comprises box body one 6, box cover one 5 and partition net 8, and box cover one 5 is covered on box body one 6 top, and partition net 8 is arranged on box body one 6 middle part movably, so The mesh size of the above-mentioned spacer 8 is 6～8mm.

As Fig. 3, described pupation device comprises box body two 10, box cover two 9 and paper slot 11, box cover two 9 covers on box body two 10 tops, paper slot 11 is placed in box body two 10, paper slot 11 For double layer.

As shown in Fig. 4, described eclosion device packs base one 16 and cup body 14, and cup body 14 is buckled on the base one 16 upside down, and cup body 14 top surface is provided with through hole, and through hole is sealed by gauze 15, and gauze 15 meshes The size is 10-12 mesh, and the cotton ball 13 that is stained with sugar water is arranged above the through hole;

As shown in Figure 5, the mating and oviposition device includes a base 2 19, a cup body 18 and a cup cover 17, the cup body 18 is arranged on the base 2 19, the cup cover 17 is covered on the cup body 18, and the cup cover 17 tops Surface is provided with through hole, and through hole is sealed by gauze 15, and gauze 15 mesh size is 10-12 order, and the cotton ball 13 that is stained with sugar water is set above through hole, and the middle part of base two 19 is sunken downwards, and base two 19 is laid With moisturizing filter paper4. 20 among the figure is the adult of the green silk borer.

The temperature in the artificial climate chamber is 25°C±1°C, the photoperiod is 14L:10D, and the humidity is 70%±10%.

Example 2

The invention discloses an indoor rearing method of the green silk borer, which comprises the steps of raising larvae, pupae, adults and eggs, specifically, the newly hatched larvae of the green silk borer are raised in a larva raising device, and sugar gum leaves are used as food, and are raised until Pre-pupae, put them into the pupation device until they pupate; after identifying the male and female pupae, place them separately in the eclosion box, and place them in the adult feeding device in pairs after eclosion to wait for them to mate and lay eggs; collect the eggs in the adult feeding device granules, put them into a clean round plastic box with moisturizing filter paper on the bottom, spread young sugar gum leaves on top, put them in an artificial climate box, pay attention to moisturizing every day, until they hatch; after the larvae hatch, cycle the larvae according to the above steps Carry out subculture.

The specific process from newly hatched larvae to pupation is: use sugar gum tree as food material, spread it on the bottom of the device, and cover the food with leaves that are full of larvae (the newly hatched larvae will gather on the young leaves to feed) in the hatching device , after covering the box lid, it is placed in an artificial climate box to raise; the temperature in the artificial climate box is 25°C ± 1°C, the photoperiod is 14L: 10D and the humidity is 70% ± 10%; it is replaced once a day Food material, when changing the food material the next day, put the net into it, and spread fresh leaves on the net for it to eat, take out the net on the third day and clean up the insect droppings at the bottom of the box, and then put the insects on the net and Lay the leaves on the bottom of the box, and lay fresh leaves on the grid. Repeat the above steps until the larvae turn red and appear in a pre-pupation state, and then transfer them to the pupation device.

The specific process of pupation is: use a sterilized writing brush to pick the larvae in the pre-pupation state from the feeding device into the double-layer paper tank in the square pupation device, put 20 larvae in each device, and put the square plastic The box is placed in an artificial climate box, and the cocoon is made of spinning silk and paper trough until it pupates; the temperature in the artificial climate box is 25°C±1°C, the photoperiod is 14L:10D and the humidity is 70% ±10%.

The specific process of eclosion is: after the larvae in the pupation device have pupated, take out the paper trough, pull the two sides, and gently pull outwards to tear the cocoon, then take out the pupae with sterilized tweezers, and put them under the stereoscopic view. Identify male and female pupae under a microscope; put male and female pupae into sterilized eclosion boxes respectively, lay moisturizing filter paper in the box, and put cotton balls dipped in 10% sucrose water, and place the square plastic box in an artificial climate box, Until it emerges; the temperature in the artificial climate box is 25°C±1°C, the photoperiod is 14L:10D and the humidity is 70%±10%.

The specific process from mating to oviposition is as follows: place the male and female worms that have just emerged at the same age in an adult mating device, put 2-3 gum leaves and 10% sucrose water cotton balls inside, and place the mating cup in an artificial climate box. Let them mate freely until eggs appear on the cup wall or leaves, take out the males, replace the filter paper, leaves and mating cups, check whether there are eggs in the adult device every day; if so, continue to replace new filter paper, leaves and mating cups .

The specific process of hatching to larvae is as follows: collect the filter paper and leaves with eggs, cut off the eggs on the wall of the mating cup together with the cup wall, put them into a clean round plastic box with moisturizing filter paper on the bottom, and put young tenders on the top. Sugar gum leaves are placed in an artificial climate box until it hatches; the temperature in the artificial climate box is 25°C±1°C, the photoperiod is 14L:10D and the humidity is 70%±10%, and the hatched larvae Continue to carry out subculture according to the cycle of above-mentioned steps.

Example 3 The effect of the pupation place on the pupation of the green leaf borer

1. Test material:

Glass Petri dishes (15cm), disposable plastic cups, old chicle leaves, A4 white paper slots, sand (sticky: sand = 2:1), rubber bands, scissors, tweezers and other tools.

2. Test method:

Temperature: 25±1°C, RH: 70%±5%, photoperiod L:D=14:10. Insect source: Select 12 mature larvae (0.2-0.25g) that are relatively well-developed and healthy and about to pupate, 5 dishes per group.

White Paper Slot Fold the printing paper in half once along the long side to get two layers of rectangular paper, then fold the front and back alternately along the short side to get a zigzag wavy paper slot. The paper slot is a WWW shape viewed from the side, and each slot is V-shaped. Cocooning is made by using the groove walls on both sides of the V-shaped groove as support points. Therefore, we set the paper trough as two layers, and the two V-shaped troughs on the upper layer have independent walls respectively. There is also an independent wall in each V-shaped groove when cocooning in the lower floor paper groove.

If no double-layer paper chute is used, for a single-layer paper chute, a V-shaped groove with the opening facing upwards and an adjacent V-shaped groove with the opening facing downwards have a common wall, so that the common wall will be at the same time The strength to support the two cocoons is not strong enough, so that the cocoon pupation rate is low. The 1.5cm double-layer paper tank was put into a glass petri dish, and the mature larvae of the green silk borer were put into it and waited for it to pupate.

For the leaves, spread the old leaves of sugar gum tree in a layer and put them in a glass petri dish, put the mature larvae into it with sterilized tweezers, cover with a layer of old sugar gum leaves, and wait for them to pupate.

Soil Mix clay and sand with water according to a certain ratio, hold it into a ball, and it is better to disperse when it falls on the ground, and put it into a disposable plastic cup. Put the mature larva into it with sterilized tweezers, cover it with gauze and seal it with a rubber band, and wait for it to pupate.

Observe and record the state of mature larvae every day, record the pupation time, the number of deformities, deaths and successful pupation, and record the pupae weight, sex ratio and eclosion rate after pupation. The experiment was repeated three times.

3. Test results

By comparing the pupation status of the green silk borer in soil, white paper trough and leaves, the mortality rate, deformity rate and prepupation period of the green silk borer in the white paper trough were observed. It was lower than other pupation places; the weight of pupae after pupation of S. chinensis was also higher than that of other pupation places; the sex ratio of male and female was close to 1:1. The results are shown in Table 1.

Table 1. Effects of different pupation places on the pupation of Leaf Borer

Note: The data in the same column are represented (mean ± standard error), and those with different lowercase letters indicate significant differences (TUKEY method, P<0.05).

4 Conclusion

By comparing the pupation conditions of the mature larvae of the green silk borer in three different places, it can be seen that pupation in the white paper tank can not only take a short time, but also has a higher pupation rate and a heavier pupae weight. , and the eclosion rate of pupae was also the highest among the three. In addition, the success rate of pupation in the leaves of the leaf borer is relatively high, because this place is the most common place for pupation in natural populations, but compared with paper tank pupation, the materials used in this method are more special. The leaves need to be picked separately. In addition, the pupation in the leaves has a low space utilization rate and takes up a large area. As for the pupation method in the paper tank, a paper tank made of a 16-kilometer paper can hold 20 mature larvae for pupation. When taking the pupae, you only need to pull the two sides of the paper tank and gently pull it outward. , so that the cocoon is torn, and the pupa can be taken out with sterilized forceps. Therefore, in summary, the method of pupation using paper troughs is more suitable as a method for the mass reproduction of Leucopteris spp.

Claims (6)

1. a kind of indoor rearing method of silk borer, is characterized in that, comprises the raising step of larva, pupa, adult and ovum, specifically, The specific process from newly hatched larvae to prepupa is: Lay the chicle leaves on the bottom of the larva rearing device, take out the chicle leaves covered with newly hatched larvae from the hatching device and cover them on the gum leaves of the larva rearing device, then move the larva rearing device to an artificial climate box to raise, Change the food once a day. When changing the food on the second day, install the partition net in the middle of the larva rearing device, and spread fresh food on the partition net for feeding. On the third day, take out the partition net and clean the bottom of the larva rearing device. Feces, and then spread the worms and food materials on the separation net into the bottom of the larva rearing device, and spread fresh food materials on the separation net, repeat the above steps until the larva body color turns red and is in a pre-pupa state. The specific process of pupation is: Pick the larvae in the pre-pupation state from the larva rearing device to the paper trough of the pupation device, and move the pupation device to the artificial climate box. The larvae spin silk and bind the paper trough to make cocoons and pupate, and wait for pupation After the larvae in the device pupate, take out the paper trough, pull the two sides, and gently pull towards the two ends to tear the cocoon, and then take out the pupae with sterilized tweezers. The specific process of feathering is: The male and female pupae were identified under a stereoscope, and the male and female pupae were placed in two different sterile eclosion devices. The pupae were placed on moisturizing filter paper, and the eclosion device was moved to an artificial climate box until eclosion. The specific process from mating to spawning is: Move the females and males that have just emerged at the same age to the mating and oviposition device, and put the sugar gum leaves, and then move the mating and oviposition device to the artificial climate box, and let them mate freely. When the mating and oviposition device appears Take out the male worm when the eggs are in, and then take out and replace the parts of the mating and oviposition device with eggs every day until the end of oviposition; The specific process from hatching to newly hatched larvae is: Put the eggs of the green-winged silk moth into the hatching device with moisturizing filter paper on the bottom, spread the young sugar gum leaves on top, and place them in an artificial climate box until the eggs hatch into newly hatched larvae. After the larvae hatch, the larvae are subcultured according to the above-mentioned steps. 2. the indoor rearing method of the green silk borer according to claim 1, is characterized in that, described larva rearing device comprises box body one (6), box cover one (5) and spacer net (8), box Cover one (5) is covered on box body one (6) top, and partition net (8) is arranged on box body one (6) middle part movably, and the mesh size of described partition net (8) is 6～8mm. 3. the indoor rearing method of green-winged silk borer according to claim 1, is characterized in that, described pupation device comprises box body two (10), box cover two (9) and paper groove (11), box Lid two (9) are covered in box body two (10) tops, and paper groove (11) is put in the box body two (10), and paper groove (11) is double-layer. 4. the indoor rearing method of green-winged silk borer according to claim 1, is characterized in that, described eclosion device packs base one (16) and cup body (14), and cup body (14) buckles upside down on base one (16), the cup body (14) top surface is provided with a through hole, and the through hole is sealed by gauze (15), and the gauze (15) mesh size is 10-12 mesh, and the cotton ball that is stained with sugar water is set above the through hole (13); the middle part of the base one (16) is sunken downwards, and the base one (16) is covered with moisturizing filter paper (4). 5. the indoor rearing method of green-winged silkworm according to claim 1, is characterized in that, described mating and oviposition device comprises base two (19), cup body tube (18) and cup cover (17), cup The body tube (18) is arranged on the base two (19), the cup cover (17) is covered on the cup body tube (18), the top surface of the cup cover (17) is provided with a through hole, and the through hole is sealed by a gauze (15) , gauze (15) mesh size is 10-12 order, and the cotton ball (13) that is stained with sugar water is arranged above the through hole, and the middle part of base two (19) is sunken downwards, is covered with moisturizing filter paper (4) on the base two (19). ). 6. The indoor rearing method of the green-winged silk borer according to claim 1, characterized in that, the temperature in the artificial climate box is 25°C±1°C, the photoperiod is 14L:10D, and the humidity is 70% ±10%.