CN107581159A - A kind of figured cloth moths attracted by lamplight natural enemy hangs the artificial fecundation method of cocoon honeybee - Google Patents
A kind of figured cloth moths attracted by lamplight natural enemy hangs the artificial fecundation method of cocoon honeybee Download PDFInfo
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- CN107581159A CN107581159A CN201710896002.7A CN201710896002A CN107581159A CN 107581159 A CN107581159 A CN 107581159A CN 201710896002 A CN201710896002 A CN 201710896002A CN 107581159 A CN107581159 A CN 107581159A
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- honeybee
- lamplight
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Abstract
A kind of figured cloth moths attracted by lamplight natural enemy hangs the artificial fecundation method of cocoon honeybee, and the cloth moths attracted by lamplight of collection worm bag of surviving the winter is placed into raising box, sprouts wings and outstanding cocoon honeybee；Collection figured cloth moths attracted by lamplight pieces of an egg or newly hatched larvae are brought into laboratory and cultivated；Or the figured cloth moths attracted by lamplight of laboratory squamous subculture are used after figured cloth moths attracted by lamplight worm's ovum turns to larva, to be put into feeding box and raise, obtain the low instar larvae of figured cloth moths attracted by lamplight as host；By outstanding cocoon honeybee into honeybee is female, male pairing, it is introduced into raising box, allows its natural mating, and the ovipositing hosts in host；Then change to the new feeding box for having not parasitic host and relay continued access honeybee, until queen bee is dead；Parasitic wasp feeds natural feed or man-made feeds, you can sprouts wings and outstanding cocoon honeybee of future generation；Cocoon honeybee can be hanged using this method by large-scale breeding indoors, and practical biological prevention means are provided for Stand control figured cloth moths attracted by lamplight or other Lepidoptera leafeating insects.
Description
Technical field
It is specifically a kind of to parasitize the outstanding of figured cloth moths attracted by lamplight larva the present invention relates to agriculture and forestry biological control of insect pests field
The artificial fecundation method of cocoon honeybee.
Background technology
Figured cloth moths attracted by lamplightCamptoloma interiorata(Walker) it is a kind of important leafeating insect for endangering toothed oak tree,
It is the extremely strong Pest in Forest species of a kind of adaptability and fulminant.The worm is extensive in China distributed area, and the worm is to locality
Tree growth, woodland scenery can do great damage, and have a strong impact on the development of local economy and tourist industry.The flower of China's preventing and treating at present
The means of cloth moths attracted by lamplight are still more traditional, overall based on artificial physics preventing and treating and chemical prevention, and the research phase of biological control
To weaker.In order to explore figured cloth moths attracted by lamplight biological prevention, and new way and new tool are provided for preventing and treating of the worm in future.I
When studying figured cloth moths attracted by lamplight larval phase insect parasite find have a kind of parasitic wasp parasitic rate highest, it should be the Northeast's figured cloth
A kind of Dominant Natural Enemies of moths attracted by lamplight larva.The parasitic wasp is a kind of outstanding cocoon honeybee through expert appraisalMeteorussp..Up to the present,
The artificial diet technique of this outstanding cocoon honeybee is had no, related article is also there are no and delivers and be available for using for reference.
The content of the invention
, can be in room using this method it is an object of the invention to provide the artificial fecundation method that a kind of figured cloth moths attracted by lamplight hang cocoon honeybee
Interior large-scale breeding hangs cocoon honeybee, and practical biological control skill is provided for Stand control figured cloth moths attracted by lamplight or other Lepidoptera leafeating insects
Art means.
The present invention provides the artificial fecundation method that a kind of figured cloth moths attracted by lamplight natural enemy hangs cocoon honeybee, comprises the following steps：
A. honeybee kind gathers：In spring, survived the winter worm bag in field acquisition figured cloth moths attracted by lamplight, take back laboratory, be placed into raising box,
It is placed in 22-28 DEG C of temperature, relative humidity 50-70%, cultivated under conditions of the natural photoperiod, you can sprouts wings and outstanding cocoon honeybee, into honeybee
10-15% hydromel is used after emergence daily, is to be supplemented the nutrients into honeybee, to improve spawning quantity, and extends the life-span into honeybee；The
Also cocoon bee imago directly can be hanged as kind of a honeybee by the use of the figured cloth moths attracted by lamplight of squamous subculture within 2 years；
B. gather and raise the host of outstanding cocoon honeybee：Mid or late June, field acquisition figured cloth moths attracted by lamplight pieces of an egg or newly hatched larvae, will be adopted
The figured cloth moths attracted by lamplight worm's ovum collected is brought into laboratory and cultivated；Or the figured cloth moths attracted by lamplight of laboratory squamous subculture are used as host,
2~3 layers of blotting paper is spread in sterile raising box, worm's ovum is put into wherein, is placed in illumination box and cultivates after closed.Culture
22~28 DEG C of temperature, photoperiod L:D=14h:10h, under conditions of relative humidity is 50-70%.Treat that figured cloth moths attracted by lamplight worm's ovum turns to children
After worm, figured cloth moths attracted by lamplight larva is put into feeding box and raised, per box larva 10-15 heads, obtains the low instar larvae of figured cloth moths attracted by lamplight；
C. honeybee is connect：By outstanding cocoon honeybee into honeybee is female, male pairing, it is introduced into raising box, under room temperature condition and natural lighting, allows it
Natural mating, and the ovipositing hosts in host；Outstanding cocoon honeybee queen bee starts to lay eggs, and it is 10/box to connect honeybee ratio, takes out and posts after 24 hours
Main, it is 20/box to connect honeybee ratio, rear after 48 hours to take out host, is then changed to the new feeding box relaying for having not parasitic host
Continued access honeybee, until queen bee is dead；
D. parasitic wasp development management：Take out by the host of parasitism, be placed in 20-28 DEG C, photoperiod L:D=14h:10h is relatively wet
Spend to be cultivated under conditions of 50-70%, feed natural feed or man-made feeds, you can sprout wings and outstanding cocoon honeybee of future generation；
The good effect that the present invention has：
1st, the present invention is successfully realized the natural enemies of indoors artificial large-scale breeding figured cloth moths attracted by lamplight ----outstanding cocoon honeybee so that the honeybee it is big
Amount breeding is not subject to seasonal restrictions, and can be effectively improved labor productivity and equipment service efficiency, saved cost, ensure that parasitic wasp
Long-term substantial amounts of source of supply, be advantageous to carry out permanently effective biological control to figured cloth moths attracted by lamplight or other Lepidoptera leafeating insects.
2nd, technical scheme provided by the invention make use of natural host using raising, be advantageous to keep the parasitic energy of outstanding cocoon honeybee
Power, ensure control effect in forest, and reduce the frequency of natural enemy insect Rejuvenate species, at the same using man-made feeds host plant for rearing into
It work(, efficiently avoid because of season reason, be not easy to obtain natural feed（Mongolian oak and Quercus liaotungensis）The problem of.
3rd, the wild type of the outstanding cocoon honeybee that the present invention is bred, its female, male size and field acquisition hangs cocoon honeybee
Body difference in size unobvious, in addition, in life-span, male and female sex ratio etc. also with wild type without marked difference.Experiment shows,
The filial generation of the filial generation and its natural propagation of the outstanding cocoon honeybee of the inventive method breeding is very much like.
4th, the rearing method of outstanding cocoon honeybee of the invention is simple and easy, is particularly adapted to indoor feeding and observes its life
History, even if keeper can also learn simultaneously independent operation in a short time without relevant professional knowledge.
Below by way of specific embodiment, the present invention is described in further detail, but does not carry out any limit to the present invention with this
System.
Embodiment
Embodiment 1
A. annual April in spring is upper, the middle ten days, collection figured cloth moths attracted by lamplight survived the winter worm bag in Quercus liaotungensis or Mongolian oak standing forest in the wild, takes back
Laboratory, it is placed into sterilized raising box,（Density is per 10, box）, it is placed in 22-28 DEG C of temperature, relative humidity 50-
70%th, cultivated under conditions of the natural photoperiod, you can sprout wings and outstanding cocoon honeybee, into bee eclosion after daily use diameter 1cm rayon balls
Plus 10% hydromel, make cotton balls moistening but no liquid is oozed out, be put into feeding box, be to be supplemented the nutrients into honeybee, to improve spawning
Quantity, and extend the life-span into honeybee.Second Year also directly can hang cocoon bee imago as kind of a honeybee by the use of the figured cloth moths attracted by lamplight of squamous subculture.
B. mid or late June adult field acquisition figured cloth moths attracted by lamplight pieces of an egg or newly hatched larvae, the figured cloth moths attracted by lamplight worm's ovum that will be collected
Bring into laboratory and cultivate.Or the figured cloth moths attracted by lamplight of laboratory squamous subculture are used as host, spread 2 in sterile raising box~
3 layers of blotting paper, worm's ovum is put into wherein, is placed in illumination box and cultivates after closed.22~28 DEG C of cultivation temperature, photoperiod
L:D=14h:10h, under conditions of relative humidity is 50-70%.After figured cloth moths attracted by lamplight worm's ovum turns to larva, by figured cloth moths attracted by lamplight larva
It is put into feeding box and raises.Per box larva 10, fresh Quercus liaotungensis, Mongolian oak tender leaf are changed daily or uses fall webworms larva
Feed is raised（The fall webworms larvae feeds that purchase is developed using China Forestry Science Research Institute, the patent No.：
200510127628.9；）, and excrement, residual feed and dead larva in cleaning box.Obtain the low instar larvae of figured cloth moths attracted by lamplight
（1-2 ages）.
C. outstanding cocoon honeybee is introduced into raising box into honeybee is female, male pairing, under room temperature condition and natural lighting, allow its from
So mating, and the ovipositing hosts in host.The quantitative proportion of queen bee and host are 1：10（One raising box）.Outstanding cocoon honeybee queen bee opens
Begin to take out outstanding cocoon honeybee after laying eggs 24 hours into honeybee, then change to the new feeding box for having not parasitic host and relay continued access honeybee, directly
It is dead to queen bee.
D. take out by the host of parasitism, be placed in 20-28 DEG C, photoperiod L:D=14h:10h, relative humidity 50-70%
Under conditions of cultivate, change fresh Quercus liaotungensis, Mongolian oak tender leaf or fall webworms larvae feeds daily and raised, and cleaning box
The larva of interior excrement, residual feed and death.It can sprout wings and outstanding cocoon honeybee of future generation.
Feeding box is organic plastics box, and length, width and height size is 12cm × 9cm × 4.5cm, box top uncovered, covers 30 Muchuangs
Gauze.
Embodiment 2
A. annual April in spring is upper, the middle ten days, collection figured cloth moths attracted by lamplight survived the winter worm bag in Quercus liaotungensis or Mongolian oak standing forest in the wild, takes back
Laboratory, it is placed into sterilized raising box（Density is per 20, box）, it is placed in 22-28 DEG C of temperature, relative humidity 50-
70%th, cultivated under conditions of the natural photoperiod, you can sprout wings and outstanding cocoon honeybee, into bee eclosion after daily use diameter 1cm rayon balls
Plus 15% hydromel, make cotton balls moistening but no liquid is oozed out, be put into feeding box, be to be supplemented the nutrients into honeybee, to improve spawning
Quantity, and extend the life-span into honeybee.Second Year also directly can be used as kind of a honeybee by the use of the outstanding cocoon bee imago of squamous subculture.
B. mid or late June adult field acquisition figured cloth moths attracted by lamplight pieces of an egg or newly hatched larvae, the figured cloth moths attracted by lamplight worm's ovum that will be collected
Bring into laboratory and cultivate.Or the figured cloth moths attracted by lamplight of laboratory squamous subculture are used as host, spread 2 in sterile raising box~
3 layers of blotting paper, worm's ovum is put into wherein, is placed in illumination box and cultivates after closed.22~28 DEG C of cultivation temperature, photoperiod
L:D=14h:10h, under conditions of relative humidity is 50-70%.After figured cloth moths attracted by lamplight worm's ovum turns to larva, by figured cloth moths attracted by lamplight larva
It is put into feeding box and raises.Per box larva 20, fresh Quercus liaotungensis, Mongolian oak tender leaf or fall webworms larvae feeds are changed daily
Raised.And excrement, residual feed and dead larva in cleaning box.Obtain the low instar larvae of figured cloth moths attracted by lamplight（1-2 ages）.
C. outstanding cocoon honeybee is introduced into raising box into honeybee is female, male pairing, under room temperature condition and natural lighting, allow its from
So mating, and the ovipositing hosts in host.The quantitative proportion of queen bee and host are 1：20（One raising box）.Queen bee starts to lay eggs
Outstanding cocoon honeybee is taken out after 48 hours into honeybee, then changes to the new feeding box for having not parasitic host and relays continued access honeybee, until queen bee
It is dead.
D. take out by the host larva of parasitism, be placed in 20-28 DEG C, photoperiod L:D=14h:10h, relative humidity 50-
Cultivated under conditions of 70%, change fresh Quercus liaotungensis, Mongolian oak tender leaf or fall webworms larvae feeds daily and raised, and clearly
Manage the larva of excrement, residual feed and death in box.It can sprout wings and outstanding cocoon honeybee of future generation.
Feeding box is organic plastics box, and length, width and height size is 12cm × 9cm × 4.5cm, box top uncovered, covers 30 Muchuangs
Gauze.
Claims (4)
1. a kind of figured cloth moths attracted by lamplight natural enemy hangs the artificial fecundation method of cocoon honeybee, it is characterised in that:Comprise the following steps：
A. honeybee kind gathers：In spring, survived the winter worm bag in field acquisition figured cloth moths attracted by lamplight, take back laboratory, be placed into raising box,
It is placed in 22-28 DEG C of temperature, relative humidity 50-70%, cultivated under conditions of the natural photoperiod, you can sprouts wings and outstanding cocoon honeybee, into honeybee
10% hydromel is used after emergence daily, is to be supplemented the nutrients into honeybee, to improve spawning quantity, and extension is into the life-span of honeybee；Second Year
Also cocoon bee imago directly can be hanged as kind of a honeybee by the use of the figured cloth moths attracted by lamplight of squamous subculture；
B. gather and raise the host of outstanding cocoon honeybee：Mid or late June, field acquisition figured cloth moths attracted by lamplight pieces of an egg or newly hatched larvae, will be adopted
The figured cloth moths attracted by lamplight worm's ovum collected is brought into laboratory and cultivated；Or the figured cloth moths attracted by lamplight of laboratory squamous subculture are used as host,
2~3 layers of blotting paper is spread in sterile raising box, worm's ovum is put into wherein, is placed in illumination box and cultivates after closed；
22~28 DEG C of cultivation temperature, photoperiod L:D=14h:10h, under conditions of relative humidity is 50-70%；
After figured cloth moths attracted by lamplight worm's ovum turns to larva, figured cloth moths attracted by lamplight larva is put into feeding box and raised, obtains the low of figured cloth moths attracted by lamplight
Instar larvae；
C. honeybee is connect：By outstanding cocoon honeybee into honeybee is female, male pairing, it is introduced into raising box, under room temperature condition and natural lighting, allows it
Natural mating, and the ovipositing hosts in host；Outstanding cocoon honeybee queen bee takes out outstanding cocoon honeybee into honeybee after starting spawning 24 hours, then change
Continued access honeybee is relayed to the new feeding box for having not parasitic host, until queen bee is dead；
D. parasitic wasp development management：Take out by the host of parasitism, be placed in 20-28 DEG C, photoperiod L:D=14h:10h is relatively wet
Spend to be cultivated under conditions of 50-70%, feed natural feed or man-made feeds, you can sprout wings and outstanding cocoon honeybee of future generation.
2. figured cloth moths attracted by lamplight according to claim 1 hang cocoon honeybee artificial fecundation method, it is characterised in that outstanding cocoon honeybee is female into honeybee
Quantitative proportion with host is 1:10-20.
3. figured cloth moths attracted by lamplight according to claim 1 hang the artificial fecundation method of cocoon honeybee, it is characterised in that described queen bee into
When worm starts spawning, it is 10/box to connect honeybee ratio, and host is taken out after 24 hours, and it is 20/box to connect honeybee ratio, rear after 48 hours to take out
Host, a collection of new host is then changed, change a collection of host within hereafter every 24 hours or 48 hours, until queen bee is dead.
4. figured cloth moths attracted by lamplight according to claim 1 hang the artificial fecundation method of cocoon honeybee, it is characterised in that feeding box is organic
Plastic casing, length, width and height size are 12cm × 9cm × 4.5cm, box top uncovered, cover 30 Muchuang gauzes.
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| CN108522430A (en) * | 2018-03-23 | 2018-09-14 | 海南大学 | A kind of indoor feeding method of common big thrips |
| CN111727936A (en) * | 2020-06-30 | 2020-10-02 | 扬州大学 | Indoor propagation method of chilo suppressalis fringed cocoon bee |
| CN111887207A (en) * | 2020-07-09 | 2020-11-06 | 武汉市园林科学研究院 | Application of paralytic function of gelechiid chogramma in artificial cultivation of natural enemy insects |
| CN113424805A (en) * | 2021-08-04 | 2021-09-24 | 重庆市畜牧科学院 | Artificial breeding method for Scleroderma stevensis |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108522430A (en) * | 2018-03-23 | 2018-09-14 | 海南大学 | A kind of indoor feeding method of common big thrips |
| CN111727936A (en) * | 2020-06-30 | 2020-10-02 | 扬州大学 | Indoor propagation method of chilo suppressalis fringed cocoon bee |
| CN111887207A (en) * | 2020-07-09 | 2020-11-06 | 武汉市园林科学研究院 | Application of paralytic function of gelechiid chogramma in artificial cultivation of natural enemy insects |
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