CN102885008A - Artificial propagation method of Coccygomimus disparis - Google Patents
Artificial propagation method of Coccygomimus disparis Download PDFInfo
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Abstract
The invention discloses an artificial propagation method of Coccygomimus disparis, comprising the following steps of a, choosing a fresh pupa of hyphantria cunea as a host, half embedding the host into wet sandy soil and putting in a raising gauze cage; b, pairing male and female adults of the Coccygomimus disparis and leading in the raising gauze cage to ensure that the adults freely mate under the conditions of room temperature and natural light, spawn and parasitically live in the host; and c, taking out the hose, culturing the host under the conditions that the temperature is 21-28 DEG C and the relative humidity is 50-70% and obtaining next-generation Coccygomimus disparis through eclosion. The method is not limited by season and capable of massively propagating the Coccygomimus disparis indoors, therefore, long-term massive supply source of the Coccygomimus disparis is guaranteed and long-term effective biological control can be carried out on lepidopterous defoliators such as woodland gypsy moth, the hyphantria cunea, pine moth and the like.
Description
Technical field
The present invention relates to insect breeding field, the specifically artificial fecundation method of a kind of gypsymoth melanoma ichneumon wasp.
Background technology
Gypsymoth melanoma ichneumon wasp (
Coccygomimus disparis(Viereck), Hymenoptera: Ichneumonidae) be the parasitic wasp of Lepidoptera (Lepidoptera) pupa, domestic known distribution in Jilin, the province such as Liaoning, Beijing, Hebei, Shanxi, Heilungkiang, Shanghai, Jiangsu, Anhui, Zhejiang, Jiangxi, Fujian, Hunan, Henan, Shaanxi, Ningxia, Yunnan, Sichuan, be distributed in Japan, Korea, Mongolia, the former Soviet Union and India abroad.
The host of gypsymoth melanoma ichneumon wasp is a lot, main parasitic pine caterpillars (
DendrolimusSpp), gypsymoth [
Lymantria dispar(Linnaeus)], fall webworm [
Hyphantria cunea(Drury)], tent caterpillar (
Malacosoma neustriaLinnaeus), the arbor-vitae poison moth [
Parocneria furva(Leech)], Clania variegata Snellen (
Cryptothelea variegataSnellen), poplar caterpillar [
Clostera anachoreta(Fabricius)], lebeda nobilis (
Lebeda nobilisWalker) etc. in the pupa of 30 various pests.According to 1993 in the investigation of Chinese Rizhao and northern Suzhou, gypsymoth melanoma ichneumon wasp main host plants be eligma narcissus (
Eligmanar cissusGramer), Clania variegata Snellen, dendrolimus spectabilis (
Dendrolimus spectabilisButler), swallowtail butterfly (Papilionidae), the highest with the parasitic rate on the eligma narcissus pupa, reach 52.33%, parasitic rate on other 3 kinds of insects be respectively 2.56%, 3.98% and 1.92%(Wang Chang expensive etc. gypsymoth melanoma ichneumon wasp biological property preliminary study. the Shandong forestry science and technology, 1996, (5): 32 ~ 33).Found when investigating in Beijing in 1996 to 1997 that gypsymoth melanoma ichneumon wasp has reached 24.3%(Feng Ji China etc. in Yongning, Yanqing to the parasitic rate of gypsymoth. the Gypsy moth in Beijing area natural enemy insect and naturally control study. forest-science, 1999,35 (2): 50 ~ 56).In the investigation on the ground such as Dalian, Yantai, Qinhuangdao, found respectively in 1996, gypsymoth melanoma ichneumon wasp can reach 9.8%(Yang Xiu minister in ancient times etc. to the natural parasitic rate of fall webworm. Dalian Area fall webworm parasitic enemy insect. and the Chinese biological control, 2001, (1): 40 ~ 42; Yang et al. Survey of the native insect natural enemies of
Hyphantria cunea(Drury) (Lepidoptera:Arctiidae) in China. Bulletin of Entomological Research, 2008,98:293 ~ 302).
Gypsymoth melanoma ichneumon wasp individuality is larger, and the long 9 ~ 18mm of body survives the winter in Host Pupae with mature larva, is generally solitary parasitism.Can finish 4 ~ 5 generations every year.In Host Pupae, become prepupa at the beginning of the late March to 4 month, pupate 24 ~ 30 days pupa time, average 27 days after 1 ~ 2 day.Various places are different period with the different emergence of temperature.Life of Adult is longer, generally all more than 30 days.The adult ability of circling in the air is strong, and certain phototaxis is arranged.After female worm sprouts wings can with the male worm mating, mating time 35 seconds did not wait to 1 minute.The preoviposition period of female worm about 3 ~ 4 days.Gypsymoth melanoma ichneumon wasp build is large, the power of circling in the air is strong, and higher to the parasitic rate of lepidopterous insects, so gypsymoth melanoma ichneumon wasp is a kind of outstanding natural enemy insect, and application potential and prospect in biological control are larger.But gypsymoth melanoma ichneumon wasp is not high in the Natural Population quantity of woodland, insect is not reached the purpose of nature control.If can realize artificial mass rearing, a large amount of release before insect arriving in pupa time then might be improved the parasitic rate to insect, realizes the control to pest population quantity.Up to the present, have no the artificial mass rearing technology of gypsymoth melanoma ichneumon wasp, also delivering there are no relevant article can be for using for reference.
Summary of the invention
Purpose of the present invention just provides the artificial fecundation method of a kind of gypsymoth melanoma ichneumon wasp, use the method indoor large-scale breeding gypsymoth melanoma ichneumon wasp, to provide the biological prevention means of practicality for Lepidoptera defoliators such as woodland control gypsymoth, fall webworm, pine caterpillars.
The object of the present invention is achieved like this:
The artificial fecundation method of gypsymoth melanoma ichneumon wasp provided by the present invention may further comprise the steps:
A, the fresh pupa of selection fall webworm are the host, and the host is partly imbedded in the wetting sandy soil, place and raise sarong;
B, with the pairing of the one-tenth honeybee male and female of gypsymoth melanoma ichneumon wasp, introduce and raise in the sarong, under room temperature condition and natural lighting, allow its natural mating and ovipositing hosts in the host;
C, take out by the host of parasitism, placing 21 ~ 28 ℃, relative moisture is to cultivate under 50 ~ 70% the condition, and can sprout wings gypsymoth melanoma ichneumon wasp of future generation.
The preparation method of the fresh pupa of described fall webworm: buy the fall webworm pupa from China Forestry Science Research Institute, 4 ~ 8 ℃ of lower preservations, through dissecting, it is unusual to have no pupa in 30 days with it, the reproduction rate that also has no gypsymoth melanoma ichneumon wasp has harmful effect, is fresh pupa.
Gypsymoth melanoma ichneumon wasp becomes the preparation method of honeybee kind honeybee: gather the fall webworm pupa that is subjected to gypsymoth ichneumon wasp parasitism annual March in spring in the open air, brings back the indoor ichneumon wasp emergence for the treatment of, can obtain into honeybee kind honeybee.
In order to guarantee that gypsymoth melanoma ichneumon wasp queen bee can more effectively lay eggs, described gypsymoth melanoma ichneumon wasp queen bee and host's the preferred 1:5 of quantitative proportion ~ 10;
For the Effective Raise parasitic rate, after beginning to lay eggs 24 hours, described gypsymoth melanoma ichneumon wasp queen bee takes out the host, then change a collection of new host, changed a collection of host in after this per 24 hours, until gypsymoth melanoma ichneumon wasp queen bee is dead.
Be placed with gypsymoth melanoma ichneumon wasp feed in the described raising sarong, this feed is that every 100ml hydromel contains pollen 0.5 ~ 2g.This feed can be adult sufficient nutrient component is provided.
The preferred feed of described gypsymoth melanoma ichneumon wasp is: its hydromel is that honey and distilled water are being made into (with reference to the forage compounding of general parasitic wasp of 20 ~ 30:100 according to mass ratio, see tight quiet monarch etc., the forest pest natural enemy insect. Beijing: China Forest publishing house, 1989), the particle diameter of pollen described in the hydromel after pulverizing is less than 100 orders, and so enough thin pollen can guarantee that ichneumon wasp can sufficient its nutrient component of picked-up.Pollen is commercial assorted pollen.
Described sandy soil preferable particle size is less than 40 purpose sandy soil.The particle of sandy soil can guarantee can comparatively to firmly fix after wetting the part health less than 40 orders and be embedded in wherein Host Pupae.
The size of described raising sarong is 40cm * 30cm * 30cm, and the width of mesh of the husky cage gauze of described raising is 20 ~ 30 orders.Sarong is too little, and adult does not have enough activity spaces.As long as the width of mesh of sarong gauze can limit the escape of adult, adopt commercially available conventional gauze to get final product.
After described gypsymoth melanoma ichneumon wasp becomes honeybee introduce to raise sarong, through natural lighting or artificial lighting every day 8-14 hour, do not change or keep its biological property similar to wild species to guarantee its natural quality.
The present invention has following beneficial effect:
1, the present invention has successfully realized indoors artificial large-scale breeding gypsymoth melanoma ichneumon wasp, so that the amount reproduction of gypsymoth melanoma ichneumon wasp is not subject to seasonal restrictions, energy Effective Raise labor productivity and equipment service efficiency, saved cost, guaranteed the long-term a large amount of source of supply of parasitic wasp, be conducive to the Lepidoptera defoliators such as gypsymoth, fall webworm, pine caterpillars are carried out permanently effective biological control.
2, technical scheme provided by the invention adopts the fresh pupa of fall webworm as the vector of parasitic wasp; utilized natural host; be conducive to keep the parasitic ability of gypsymoth melanoma ichneumon wasp; guarantee control effect in forest; and reduced the frequency of natural enemy insect Rejuvenate species; the feeding habits of fall webworm are very assorted simultaneously; relevant large-scale breeding technology is comparative maturity also; be easy to obtain a large amount of mature larva and pupa by indoor feeding, have more low cost production in Hosts than other host as breeding.
3, the gypsymoth melanoma ichneumon wasp that breeds of the present invention, it is female, the wild type gypsymoth melanoma ichneumon wasp Individual Size difference of the size of male and field acquisition is not obvious, in addition, at aspects such as life-span, male and female sex ratios also with wild type gypsymoth melanoma ichneumon wasp without marked difference.Experiment shows that the filial generation of the gypsymoth melanoma ichneumon wasp of the inventive method breeding is very similar to the filial generation of gypsymoth melanoma ichneumon wasp natural propagation.
Below the present invention is described in further detail by specific embodiment, but with this present invention is not carried out any restriction.
Embodiment
Embodiment 1
A, the fresh pupa of selection fall webworm are the host, then cut-off directly is the culture dish of 9cm, in the thickness of packing into be the sandy soil of 0.8 ~ 1cm, the sandy soil particle diameter is less than 40 orders, in this culture dish, add the next wetting sandy soil of 8 ~ 12ml distilled water, again the host is partly imbedded in the moistening sandy soil, this culture dish is put into raised sarong, preferred size of raising sarong is 40cm * 30cm * 30cm, and the width of mesh of raising the sarong gauze is 20 ~ 30 orders.
Cut-off directly is the culture dish of 35 ~ 60cm simultaneously, the bottom spreads one deck filter paper, splashing into several distilled water makes filter paper moistening, but can not there be water droplet to ooze out, (this feed is that every 100ml hydromel contains pollen 0.5g to get the feed of 2 ~ 3ml gypsymoth melanoma ichneumon wasp, the particle diameter of pollen is less than 100 orders, and its hydromel is that honey and distilled water are being made into of 20:100 according to mass ratio.) be added on the absorbent cotton ball that diameter is 1cm, the wetting but no liquid of cotton balls is oozed out, put into this culture dish, then this culture dish is put into and raised sarong.
B, with the pairing of the one-tenth honeybee male and female of gypsymoth melanoma ichneumon wasp, introduce and raise in the sarong, implement natural lighting to raising sarong every day.At ambient temperature, allow its natural mating; Queen bee ovipositing hosts in the host of the gypsymoth melanoma ichneumon wasp of post-coitum, gypsymoth melanoma ichneumon wasp queen bee and host's quantitative proportion is 1:5.
C, parasitic wasp were taken out the host after beginning to lay eggs 24 hours, and placing 21 ℃, relative moisture is to cultivate under 60% the condition, and after 28 days, can sprout wings gypsymoth melanoma ichneumon wasp of future generation and become honeybee.Change simultaneously a collection of new host, changed a collection of host in after this per 24 hours, until parasitic wasp is dead.
Embodiment 2
A, the fresh pupa of selection fall webworm are the host, then cut-off directly is the culture dish of 9cm, in the thickness of packing into be the sandy soil of 0.8 ~ 1cm, the sandy soil particle diameter is less than 40 orders, in this culture dish, add the next wetting sandy soil of 8 ~ 12ml distilled water, again the host is partly imbedded in the moistening sandy soil, this culture dish is put into raised sarong, preferred size of raising sarong is 40cm * 30cm * 30cm, and the width of mesh of raising the sarong gauze is 20 ~ 30 orders.
Cut-off directly is the culture dish of 35 ~ 60cm simultaneously, the bottom spreads one deck filter paper, splashing into several distilled water makes filter paper moistening, but can not there be water droplet to ooze out, (this feed is that every 100ml hydromel contains pollen 1g to get the feed of 2 ~ 3ml gypsymoth melanoma ichneumon wasp, the particle diameter of pollen is less than 100 orders, and its hydromel is that honey and distilled water are being made into of 25:100 according to mass ratio.) be added on the absorbent cotton ball that diameter is 1cm, the wetting but no liquid of cotton balls is oozed out, put into this culture dish, then this culture dish is put into and raised sarong.
B, with the pairing of the one-tenth honeybee male and female of gypsymoth melanoma ichneumon wasp, introduce and raise in the sarong, carry out natural lighting to raising sarong every day.At ambient temperature, allow its natural mating; Queen bee ovipositing hosts in the host of the gypsymoth melanoma ichneumon wasp of post-coitum, gypsymoth melanoma ichneumon wasp queen bee and host's quantitative proportion is 1:8.
C, parasitic wasp were taken out the host after beginning to lay eggs 24 hours, and placing 25 ℃, relative moisture is to cultivate under 60% the condition, and after 21 days, can sprout wings gypsymoth melanoma ichneumon wasp of future generation and become honeybee.Change simultaneously a collection of new host, changed a collection of host in after this per 24 hours, until parasitic wasp is dead.
Embodiment 3
A, the fresh pupa of selection fall webworm are the host, then cut-off directly is the culture dish of 9cm, in the thickness of packing into be the sandy soil of 0.8 ~ 1cm, the sandy soil particle diameter is less than 40 orders, in this culture dish, add the next wetting sandy soil of 8 ~ 12ml distilled water, again the host is partly imbedded in the moistening sandy soil, this culture dish is put into raised sarong, preferred size of raising sarong is 40cm * 30cm * 30cm, and the width of mesh of raising the sarong gauze is 20 ~ 30 orders.
Cut-off directly is the culture dish of 35 ~ 60cm simultaneously, one deck filter paper is put in the bottom, splashing into several distilled water makes filter paper moistening, but can not there be water droplet to ooze out, (this feed is that every 100ml hydromel contains pollen 2g to get the feed of 2 ~ 3ml gypsymoth melanoma ichneumon wasp, the particle diameter of pollen is less than 100 orders, and its hydromel is that honey and distilled water are being made into of 30:100 according to mass ratio.) be added on the absorbent cotton ball that diameter is 1cm, the wetting but no liquid of cotton balls is oozed out, put into this culture dish, then this culture dish is put into and raised sarong.
B, with the pairing of the one-tenth honeybee male and female of gypsymoth melanoma ichneumon wasp, introduce and raise in the sarong, keep natural lighting to raising sarong every day.Under indoor conditions, allow its natural mating; Queen bee ovipositing hosts in the host of the gypsymoth melanoma ichneumon wasp of post-coitum, gypsymoth melanoma ichneumon wasp queen bee and host's quantitative proportion is 1:10.
C, parasitic wasp were taken out the host after beginning to lay eggs 24 hours, and placing 28 ℃, relative moisture is to cultivate under 60% the condition, and after 17 days, can sprout wings gypsymoth melanoma ichneumon wasp of future generation and become honeybee.Change simultaneously a collection of new host, changed a collection of host in after this per 24 hours, until parasitic wasp is dead.
Embodiment 5:
Individual Size: each 10 of the male and female gypsymoth melanoma ichneumon wasps of the present invention's breeding and each 10 of the gypsymoth melanoma ichneumon wasps of field acquisition are measured respectively size, obtain data, female, the drone Individual Size of the present invention's breeding are respectively the long 1.21cm of average body, 1.17cm, female, the drone Individual Size of wild type are respectively the long 1.14cm of average body, 1.15cm, check through T, the result shows, the size of female, the male of the gypsymoth melanoma ichneumon wasp that the present invention breeds and the wild type gypsymoth melanoma ichneumon wasp Individual Size difference of field acquisition are not obvious.
Life-span: the gypsymoth melanoma ichneumon wasp drone life-span average out to of the present invention's breeding 25.05 days, queen bee life-span average out to 35.75 days.Can satisfy the needs as the biological and ecological methods to prevent plant disease, pests, and erosion factor.
Male and female sex ratio: become the female-male proportion of honeybee to compare to the indoor type of the present invention's breeding and the gypsymoth melanoma ichneumon wasp of wild type.Respectively get 200 adults, check that it is female male.Data are through multiple analysis method (One Way Anova, the SPSS statistical software) carries out statistical comparison, the result shows, (female: (female: hero=1.90:1) does not have significant difference (F=1.725 for hero=1.60:1) and the average female and male ratio of wild type gypsymoth melanoma ichneumon wasp through the average female and male ratio of the gypsymoth melanoma ichneumon wasp of artificial feeding among the present invention
P＞0.05).
Claims (7)
1. the artificial fecundation method of a gypsymoth melanoma ichneumon wasp is characterized in that it comprises the steps:
A, the fresh pupa of selection fall webworm are the host, and the host is partly imbedded in the wetting sandy soil, place and raise sarong;
B, with the pairing of the one-tenth honeybee male and female of gypsymoth melanoma ichneumon wasp, introduce and raise in the sarong, under room temperature condition and natural lighting, allow its pangamy and ovipositing hosts in the host;
C, take out by the host of parasitism, placing 21 ~ 28 ℃, relative moisture is to cultivate under 50 ~ 70% the condition, and can sprout wings gypsymoth melanoma ichneumon wasp of future generation.
2. the artificial fecundation method of gypsymoth melanoma ichneumon wasp according to claim 1 is characterized in that the female one-tenth honeybee of gypsymoth melanoma ichneumon wasp and host's quantitative proportion is 1:5 ~ 10.
3. the artificial fecundation method of gypsymoth melanoma ichneumon wasp according to claim 1 and 2, it is characterized in that taking out the host after the female bee imago of described gypsymoth melanoma ichneumon wasp began to lay eggs 24 hours, then change a collection of new host, after this changed a collection of host in per 24 hours, until gypsymoth melanoma ichneumon wasp queen bee is dead.
4. the artificial fecundation method of gypsymoth melanoma ichneumon wasp according to claim 1 and 2 is characterized in that being placed with in the described raising sarong gypsymoth melanoma ichneumon wasp feed, and this feed is to contain pollen 0.5 ~ 2g in every 100ml hydromel.
5. the artificial fecundation method of gypsymoth melanoma ichneumon wasp according to claim 4, it is characterized in that described gypsymoth melanoma ichneumon wasp feed, its hydromel is that honey and distilled water are made into by 20 ~ 30:100 according to mass ratio, and the particle diameter of pollen described in the hydromel after grinding is less than 100 orders.
6. the artificial fecundation method of gypsymoth melanoma ichneumon wasp according to claim 1 and 2 is characterized in that described sandy soil particle diameter is less than 40 orders.
7. the artificial fecundation method of gypsymoth melanoma ichneumon wasp according to claim 1 and 2, the size that it is characterized in that described raising sarong is 40cm * 30cm * 30cm, the width of mesh of the husky cage gauze of described raising is 20 ~ 30 orders.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105145495A (en) * | 2015-09-10 | 2015-12-16 | 延安大学 | Artificial breeding method for jujube tree porthesia similis |
| CN105900933A (en) * | 2016-05-12 | 2016-08-31 | 南京林业大学 | Method for increasing sex proportion of offspring of anastatus disparis |
| CN107581159A (en) * | 2017-09-28 | 2018-01-16 | 沈阳农业大学 | A kind of figured cloth moths attracted by lamplight natural enemy hangs the artificial fecundation method of cocoon honeybee |
| CN113080149A (en) * | 2021-05-11 | 2021-07-09 | 武汉市华明达蜂业有限公司 | Breeding and breeding method of high-yield bee with royal jelly and high-yield honey |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1196878A (en) * | 1998-05-22 | 1998-10-28 | 沈阳农业大学 | Tech. of breeding Tetrastichidae by utilizing tussah chrysalis |
| CN1511450A (en) * | 2002-12-31 | 2004-07-14 | 杨忠岐 | Biological preventing and controlling of American white moth by chouioia cunea yang |
| CN101785445A (en) * | 2010-03-26 | 2010-07-28 | 段东红 | Scleroderma guani breeding method |
| CN101904320A (en) * | 2010-07-27 | 2010-12-08 | 中山大学 | Method for breeding a large number of microplitis bicoloratus |
-
2012
- 2012-10-31 CN CN2012104243075A patent/CN102885008A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1196878A (en) * | 1998-05-22 | 1998-10-28 | 沈阳农业大学 | Tech. of breeding Tetrastichidae by utilizing tussah chrysalis |
| CN1511450A (en) * | 2002-12-31 | 2004-07-14 | 杨忠岐 | Biological preventing and controlling of American white moth by chouioia cunea yang |
| CN101785445A (en) * | 2010-03-26 | 2010-07-28 | 段东红 | Scleroderma guani breeding method |
| CN101904320A (en) * | 2010-07-27 | 2010-12-08 | 中山大学 | Method for breeding a large number of microplitis bicoloratus |
Non-Patent Citations (1)
| Title |
|---|
| 孙守慧等: "白蛾周氏啮小蜂成虫补充营养及低温贮藏虫期的研究", 《中国生物防治学报》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105145495A (en) * | 2015-09-10 | 2015-12-16 | 延安大学 | Artificial breeding method for jujube tree porthesia similis |
| CN105145495B (en) * | 2015-09-10 | 2017-10-27 | 延安大学 | Jujube tree steals the artificial breeding method of poison moth |
| CN105900933A (en) * | 2016-05-12 | 2016-08-31 | 南京林业大学 | Method for increasing sex proportion of offspring of anastatus disparis |
| CN107581159A (en) * | 2017-09-28 | 2018-01-16 | 沈阳农业大学 | A kind of figured cloth moths attracted by lamplight natural enemy hangs the artificial fecundation method of cocoon honeybee |
| CN113080149A (en) * | 2021-05-11 | 2021-07-09 | 武汉市华明达蜂业有限公司 | Breeding and breeding method of high-yield bee with royal jelly and high-yield honey |
| CN113080149B (en) * | 2021-05-11 | 2023-02-03 | 武汉市华明达蜂业有限公司 | Breeding and breeding method of high-yield bee with royal jelly and high-yield honey |
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