CN105145495B - Jujube tree steals the artificial breeding method of poison moth - Google Patents
Jujube tree steals the artificial breeding method of poison moth Download PDFInfo
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- CN105145495B CN105145495B CN201510573938.7A CN201510573938A CN105145495B CN 105145495 B CN105145495 B CN 105145495B CN 201510573938 A CN201510573938 A CN 201510573938A CN 105145495 B CN105145495 B CN 105145495B
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- poison moth
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The invention discloses the artificial breeding method that a kind of jujube tree steals poison moth, detailed process is：Poison moth ovum will be stolen and be placed in raising box and hatched；Wait stealing poison moth egg hatching into after larva, feed is put into box to raising, and its top is blocked with gobo；Record raises humiture in box sooner or later daily, and the replacing speed of feed is daily 3~5 times, is raised；Count dead larvae number, number of times of casting off a skin, a number of cocooing within every two days, and the larva cocoond on the same day is placed into bottom and be covered with the raising box of 3~5mm wet soils, its top is blocked with gobo, until stealing poison moth emerging adult, is produced.The present invention, using artificial breeding method, realizes the large-scale breeding for stealing poison moth by the use of the jujube leaf, silk flosssilk wadding wood leaf and Chinese cabbage leaf easily obtained as feed, provides basis for novel toxin development and utilization, and can provide target spot test worm for the research such as pharmacology.
Description
Technical field
The invention belongs to technical field of insect feeding, and in particular to a kind of jujube tree steals the artificial breeding method of poison moth.
Background technology
Steal poison moth (Porthesia similis) also known as mulberry poison moth, yellow tail poison moth, category Lepidoptera (Lepidoptera) poison
Moth section (Lymantriidae), is a kind of polyphagy insect, and the country is distributed mainly on northeast, North China, northwest and Central China；State
It is outer to be distributed mainly on Japan, the Korea peninsula and Russia.Oak class, Chinese ash, willow, Chinese parasol tree, hawthorn, apple can be seriously endangered by stealing poison moth
Really, a variety of shrubs such as mulberry, oil tea, can be by leaf eat everything up during serious generation；However, poison moth whole body tool many places poison gland is stolen,
Human contact can trigger dermatitis to the malicious hair stolen on poison moth, and (Li Deng Gansu Agriculture Universities of kingdom are learned for the lymph inflammation that causes having
Report, 2011,04:53-56；The close northwests gardening (fruit tree) of Fan Chang, 2011,04:29-30).Therefore high-volume breeding is conducive to its big
Amount is gathered venom, for toxicity, pharmaceutical research, and is conducive to the development and utilization of novel toxin medicine, preparation.Meanwhile, relative to it
His experimental animal, steals the relatively low feeding cost of poison moth, target spot test worm can be provided for pharmaceutical research.
The content of the invention
It is an object of the invention to provide the artificial breeding method that a kind of jujube tree steals poison moth, trained by the method for artificial breeding
Bring batch up and steal poison moth, provide basis for novel toxin development and utilization, and target spot examination can be provided for the research such as pharmacology
Worm.
The technical solution adopted in the present invention is that jujube tree steals the artificial breeding method of poison moth, specifically implements according to the following steps：
Step 1：Poison moth ovum will be stolen and be placed in raising box and hatched；
Step 2：Wait stealing poison moth egg hatching into after larva, feed is put into box to raising, and shading fabric width is used at its top
It is stifled；Record raises humiture in box sooner or later daily, and the replacing speed of feed is daily 3~5 times, is raised；
Step 3：Every two days statistics dead larvae number, number of times of casting off a skin, a number of cocooing, and by the larva cocoond on the same day
It is placed into bottom to be covered with the raising box of 3~5mm wet soils, its top is blocked with gobo, until stealing poison moth emerging adult, i.e.,
.
It is of the invention to be further characterized in that,
In step 1, the bottom for raising box is equipped with the wet soil that thickness is 2~5mm.
Wet soil is through obtained by 30~50min of ultra violet lamp.
In step 2, feed is one kind in silk flosssilk wadding wood leaf, jujube leaf or Chinese cabbage leaf.
In step 2, it is 26~30 DEG C to raise temperature in box, and relative humidity is 60~70%.
Gained adult is placed on bottom and is equipped with captive breeding in the open-top receptacle of 1~2mm wet soils；Put in open-top receptacle
It is equipped with robber's poison moth ovum collecting rod and nutritional supplementation liquid.
Steal hardboard or branch bar that poison moth ovum collecting rod is monolayer medical gauze wrapped.
Nutritional supplementation liquid is that concentration is 10% syrup or 3% hydromel.
The beneficial effects of the invention are as follows by the use of the jujube leaf, silk flosssilk wadding wood leaf and Chinese cabbage leaf easily obtained as feed, using people
Work mating system, realizes the large-scale breeding for stealing poison moth, provides basis for novel toxin development and utilization, and can be medicine
The research such as Neo-Confucianism provides target spot test worm.
Brief description of the drawings
Fig. 1 is the long variation diagram of body for the robber poison moth that the embodiment of the present invention 1 is bred；
Fig. 2 is the changes of weight figure for the robber poison moth that the embodiment of the present invention 1 is bred；
Fig. 3 is the long variation diagram of body for the robber poison moth that the embodiment of the present invention 2 is bred；
Fig. 4 is the changes of weight figure for the robber poison moth that the embodiment of the present invention 2 is bred；
Fig. 5 is the long variation diagram of body for the robber poison moth that the embodiment of the present invention 3 is bred；
Fig. 6 is the changes of weight figure for the robber poison moth that the embodiment of the present invention 3 is bred.
Embodiment
The present invention is described in detail with reference to the accompanying drawings and detailed description.
Jujube tree of the present invention steals the artificial breeding method of poison moth, specifically implements according to the following steps：
Step 1：Poison moth ovum will be stolen and be placed in raising box and hatched；
Step 2：Wait stealing poison moth egg hatching into after larva, feed is put into box to raising, and shading fabric width is used at its top
It is stifled；Record raises humiture in box sooner or later daily, and the replacing speed of feed is daily 3~5 times, is raised；
Step 3：Every two days statistics dead larvae number, number of times of casting off a skin, a number of cocooing, and by the larva cocoond on the same day
It is placed into bottom to be covered with the raising box of 3~5mm wet soils, its top is blocked with gobo, until stealing poison moth emerging adult, i.e.,
.Wherein：
In step 1, the bottom for raising box is equipped with the wet soil that thickness is 2~5mm.
Wet soil is through obtained by 30~50min of ultra violet lamp.
In step 2, feed is one kind in silk flosssilk wadding wood leaf, jujube leaf or Chinese cabbage leaf.
In step 2, it is 26~30 DEG C to raise temperature in box, and relative humidity is 60~70%.
Gained adult is placed on bottom and is equipped with captive breeding in the open-top receptacle of 1~2mm wet soils；Put in open-top receptacle
It is equipped with robber's poison moth ovum collecting rod and nutritional supplementation liquid.Wherein, the hardboard that poison moth ovum collecting rod is monolayer medical gauze wrapped is stolen
Or branch bar；Nutritional supplementation liquid is that concentration is 10% syrup or 3% hydromel.
Embodiment 1
By feed of silk flosssilk wadding wood leaf, poison moth is stolen in breeding：
Step 1,0.6L crisper is taken, its bottom is spread into about 2-5mm soil layer is used for moisturizing, places it in purple
Sterilized 30 minutes under outer lamp.
Step 2, the crisper bottom in step 1 is divided into six zonules, each region inserts the fresh silk flosssilk wadding wood of 2-3 pieces
Leaf, uniformly sprays appropriate water moisturizing.
Step 3, it is put into be covered with black cloth after larva and tightens mouth with rubber band.
Step 4, room temperature humidity is recorded sooner or later daily, it is 26~30 DEG C to control its temperature, relative humidity is 60~70%, and
Silk flosssilk wadding wood leaf is changed in time.
Step 5, dead number, number of times of casting off a skin, number of cocooing are counted within every two days once.The larva cocoond on the same day is placed into
Bottom is covered with the crisper of 3-5mm wet soils, and covering rubber band with black cloth seals.
Step 6, the UP pipes collection with 3-5ml after adult is turned into, is placed into during collection before the window of closing, single
Collect.
Step 7, adult enters after UP pipes, pins the mouth of pipe with thumb rapidly, is then put into 1000ml beaker rapidly.
Step 8, beaker bottom spreads about 1-2mm wet soil, and on beaker one side, oblique cutting enters to be wrapped with the hard of hospital gauze (individual layer)
Cardboard is apart from rim of a cup about 1.5cm (being used for collecting robber poison moth ovum).
Step 9, (or 3% hydromel) equipped with 10% syrup sterilized penicillin is put into the another side of beaker
Bottle, is put into absorbent cotton in this penicillin bottle or filter paper exceeds bottle 2-3cm.
Step 10, beaker mouthful covers rubber band with black cloth and sealed.
Step 11, after after Adult worms producting eggs, gauze is changed in time.
Step 12, the gauze with ovum is put into be covered with the crisper of 1-2mm wet soil and hatched.The second generation after hatching
Larva treats that next time, experiment was used.
The long variation diagram of body for the robber poison moth that the present embodiment is bred is as shown in Figure 1；The changes of weight for stealing poison moth is as shown in Figure 2；
Silk flosssilk wadding wood leaf is that feed raises robber poison moth as can be seen from Figure, and its body weight gain is dramatically increased after raising 9 days.
Embodiment 2
By feed of jujube leaf, poison moth is stolen in breeding：
Step 1,0.6L crisper is taken, its bottom is spread into about 2-5mm soil layer is used for moisturizing, places it in purple
Sterilized 30 minutes under outer lamp.
Step 2, the crisper bottom in step 1 is divided into six zonules, each region inserts the fresh Jujube Leaves of 2-3 pieces
Son, uniformly sprays appropriate water moisturizing.
Step 3, it is put into after larva and fresh-keeping box mouth is covered with black cloth, is tightened mouth with rubber band.
Step 4, room temperature humidity is recorded sooner or later daily, it is 26~30 DEG C to control its temperature, relative humidity is 60~70%, and
Jujube leaf is changed in time.
Step 5, dead number, number of times of casting off a skin, number of cocooing are counted within every two days once.The larva cocoond on the same day is placed into
Bottom is covered with the crisper of 3-5mm wet soils, and covering sturdy pines band with black cloth seals.
Step 6, the UP pipes collection with 3-5ml after adult is turned into, is placed into during collection before the window of closing, single
Collect.
Step 7, adult enters after UP pipes, pins the mouth of pipe with thumb rapidly, is then put into 1000ml beaker rapidly.
Step 8, beaker bottom spreads about 1-2mm wet soil, and on beaker one side, oblique cutting enters to be wrapped with the hard of hospital gauze (individual layer)
Cardboard is apart from rim of a cup about 1.5cm (being used for collecting robber poison moth ovum).
Step 9, (or 3% hydromel) equipped with 10% syrup sterilized penicillin is put into the another side of beaker
Bottle, is put into absorbent cotton in this penicillin bottle or filter paper exceeds bottle 2-3cm.
Step 10, beaker mouthful covers sturdy pines band with black cloth and sealed.
Step 11, after after Adult worms producting eggs, gauze is changed in time.
Step 12, the gauze with ovum is put into be covered with the crisper of 1-2mm wet soil and hatched.The second generation after hatching
Larva treats that next time, experiment was used.
The long variation diagram of body for the robber poison moth that the present embodiment is bred is as shown in Figure 3；The changes of weight for stealing poison moth is as shown in Figure 4；
Raised as can be seen from Figure by feed of jujube leaf and steal poison moth, precursor length is increased rapidly within 18 days, and body weight is then slowly increased；With
Time lengthening body length is gradually in the stage of stable development, and body weight is then in quick weightening.
Embodiment 3
By feed of Chinese cabbage leaf, poison moth is stolen in breeding：
Step 1, Chinese cabbage leaf purchased in market is cleaned 2-3 times with clear water, it is stand-by.
Step 2,0.6L crisper is taken, its bottom is spread into about 2-3mm soil layer is used for moisturizing, places it in ultraviolet
Sterilized 30 minutes under lamp.
Step 3, cassette bottom part is six zonules, and each region keeps flat 2-3 piece fresh white dish leafs.
Step 4, it is put into be covered with black cloth after larva and tightens mouth with rubber band.
Step 5, room temperature humidity is recorded sooner or later daily, it is 26~30 DEG C to control its temperature, relative humidity is 60~70%, and
Chinese cabbage leaf is changed in time.
Step 6, dead number, number of times of casting off a skin, number of cocooing are counted within every two days once.The larva cocoond on the same day is placed into
Bottom is covered with the crisper of 3-5mm wet soils, and covering sturdy pines band with black cloth seals.
Step 7, the UP pipes collection with 3-5ml after adult is turned into, is placed into during collection before the window of closing, single
Collect.
Step 8, adult enters after UP pipes, pins the mouth of pipe with thumb rapidly, is then put into 1000ml beaker rapidly.
Step 9, beaker bottom spreads about 1-2mm wet soil, and on beaker one side, oblique cutting enters to be wrapped with the hard of hospital gauze (individual layer)
Cardboard is apart from rim of a cup about 1.5cm (being used for collecting robber poison moth ovum).
Step 10, (or 3% hydromel) equipped with 10% syrup sterilized penicillin is put into the another side of beaker
Bottle, is put into absorbent cotton in this penicillin bottle or filter paper exceeds bottle 2-3cm.
Step 11, beaker mouthful covers sturdy pines band with black cloth and sealed.
Step 12, after after Adult worms producting eggs, gauze is changed in time.
Step 13, the gauze with ovum is put into be covered with the crisper of 1-2mm wet soil and hatched.The second generation after hatching
Larva treats that next time, experiment was used.
The long variation diagram of body for the robber poison moth that the present embodiment is bred is as shown in Figure 5；The changes of weight for stealing poison moth is as shown in Figure 6；
As can be seen from Figure as Chinese cabbage leaf is that feed raises robber poison moth, its body length and body weight gradually increase as feeding time is elapsed
Plus, and rate of rise was accelerated since the 9th day.
Claims (4)
1. jujube tree steals the artificial breeding method of poison moth, it is characterised in that specifically implement according to the following steps：
Step 1：Poison moth ovum will be stolen and be placed in raising box and hatched；
Wherein, the bottom for raising box is equipped with the wet soil that thickness is 2~5mm；
The wet soil is through obtained by 30~50min of ultra violet lamp；
Step 2：Wait stealing poison moth egg hatching into after larva, feed is put into box to raising, and its top is blocked with gobo；Often
Humiture in its box of record raising sooner or later, the replacing speed of feed is daily 3~5 times, is raised；
Feed is one kind in silk flosssilk wadding wood leaf, jujube leaf or Chinese cabbage leaf；
It is 26~30 DEG C to raise temperature in box, and relative humidity is 60~70%；
Step 3：Every two days statistics dead larvae number, number of times of casting off a skin, a number of cocooing, and the larva cocoond on the same day is placed
It is covered with to bottom in the raising box of 3~5mm wet soils, its top is blocked with gobo, until stealing poison moth emerging adult, is produced；
Step 4：The UP pipes collection with 3-5ml after adult is turned into, is placed into during collection before the window of closing, it is single only to receive
Collection；
Step 5：Adult enters after UP pipes, pins the mouth of pipe with thumb rapidly, is then put into 1000ml beaker rapidly, beaker mouthful
Rubber band is covered with black cloth to seal；
Step 6, after after Adult worms producting eggs, gauze is changed in time；
Step 7, the gauze with ovum is put into be covered with the crisper of 1-2mm wet soil and hatched；Second generation larva after hatching
Treat that next time, experiment was used.
2. jujube tree according to claim 1 steals the artificial breeding method of poison moth, it is characterised in that be placed on gained adult
Bottom is equipped with captive breeding in the beaker of 1~2mm wet soils；Robber's poison moth ovum collecting rod and nutritional supplementation liquid are placed with beaker.
3. jujube tree according to claim 1 steals the artificial breeding method of poison moth, it is characterised in that the robber poison moth ovum collecting
Rod is the hardboard or branch bar of monolayer medical gauze wrapped.
4. jujube tree according to claim 2 steals the artificial breeding method of poison moth, it is characterised in that the nutritional supplementation liquid is
Concentration is 10% syrup or 3% hydromel.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550489A (en) * | 2012-01-04 | 2012-07-11 | 河北大学 | Artificial propagation method for Ooencyrtus kuvanae |
| CN102885008A (en) * | 2012-10-31 | 2013-01-23 | 河北大学 | Artificial propagation method of Coccygomimus disparis |
| CN103960201A (en) * | 2014-05-12 | 2014-08-06 | 山西省农业科学院农产品质量安全与检测研究所 | Plutella xylostella indoor reproduction method |
| CN104186430A (en) * | 2014-08-14 | 2014-12-10 | 中国热带农业科学院椰子研究所 | Method for feeding a large number of laboratory test populations of Opisina arenosella Walkers |
| CN104757333A (en) * | 2015-04-23 | 2015-07-08 | 中国农业科学院植物保护研究所 | Artificial feed for asparagus caterpillar, preparation of artificial feed and feeding method |
| CN105379683A (en) * | 2015-11-13 | 2016-03-09 | 云南农业大学 | Artificial breeding method of gelechiid moths |
-
2015
- 2015-09-10 CN CN201510573938.7A patent/CN105145495B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550489A (en) * | 2012-01-04 | 2012-07-11 | 河北大学 | Artificial propagation method for Ooencyrtus kuvanae |
| CN102885008A (en) * | 2012-10-31 | 2013-01-23 | 河北大学 | Artificial propagation method of Coccygomimus disparis |
| CN103960201A (en) * | 2014-05-12 | 2014-08-06 | 山西省农业科学院农产品质量安全与检测研究所 | Plutella xylostella indoor reproduction method |
| CN104186430A (en) * | 2014-08-14 | 2014-12-10 | 中国热带农业科学院椰子研究所 | Method for feeding a large number of laboratory test populations of Opisina arenosella Walkers |
| CN104757333A (en) * | 2015-04-23 | 2015-07-08 | 中国农业科学院植物保护研究所 | Artificial feed for asparagus caterpillar, preparation of artificial feed and feeding method |
| CN105379683A (en) * | 2015-11-13 | 2016-03-09 | 云南农业大学 | Artificial breeding method of gelechiid moths |
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