CN104757333A - Artificial feed for asparagus caterpillar, preparation of artificial feed and feeding method - Google Patents
Artificial feed for asparagus caterpillar, preparation of artificial feed and feeding method Download PDFInfo
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Abstract
The invention relates to an artificial feed for asparagus caterpillar, preparation of the artificial feed and a feeding method, and belongs to the field of insect feeds. The artificial feed for asparagus caterpillar comprises the following components: wheat bran, chenopodium album powder, corn flour, ripe soybean powder, yeast powder, agar powder, distilled water, cholesterol, soybean edible oil, ascorbic acid, methyl parahydroxybenzoats and sorbic acid. Raw materials used for the feed are easy to obtain; the preparation technology is simple; the cost is relatively low; the guarantee period is relatively long (6 months); chenopodium album can reduce the viscosity of the feed, enhance the survival rate and percentage of pupation of larvae, can reduce the abnormal pupa rate, and can provide various vitamins required for the development of larva, so that the fed asparagus caterpillar has the average pupal weight of 111.8mg, has the average moth weight of 100.4mg, has the larva survival rate of 91%, and has the eclosion rate of 98%; the pupas have strong vitality, and can be stored in a refrigerator with the temperature of 5 DEG C for 7 days; the feed is relatively good in feeding effect and achieves the aim of long-term large-scale continuous rearing.
Description
Technical field
The present invention relates to insect feedstuff and breeding technology, be specifically related to the man-made feeds of beet armyworm, its preparation method and adopt the method for breeding of this feed.
Background technology
Beet armyworm spodoptera exigua
belong to Lepidoptera Lepidoptera, Noctuidae Noctuidae, be that a kind of feeding habits are mixed, the insect that the resistance to the action of a drug is strong, often causes serious threat to agricultural production and economy.In China, before the eighties in 20th century, beet armyworm is mainly caused harm the ground such as Beijing, Hebei, Henan, Shandong and Shaanxi, though there is distribution northeast, northwest and the Yangtze river basin, causes harm and does not weigh.Since the middle and later periods eighties, beet armyworm area of causing harm expands gradually, and the increasing extent of causing harm is large, and the economic loss caused is more and more serious (Yu Yousen etc., 1989 also; Jiang Xingfu, 1998; Song Guochun, 1998; Car Jin Dian etc., 2001; Zhang Zhen woods, 2004; Song Tianjun etc., 2004; Cao Chuntian etc., 2005; Tan Xiufang etc., 2005; Explain the peak etc. that jumps, 2007).
And the element task that the efficient medicament of beet armyworm and recipe determination, resistance risk assessment, natural enemy and virus expand numerous, biological control and the multinomial research such as science of heredity and Physiology and biochemistry all needs the beet exigua larvae of large batch of normal development, neat and consistent.The domestic and international development to beet exigua larvae man-made feeds and method for breeding thereof have carried out large quantifier elimination at present.Though the man-made feeds effect of open report is better, the following shortcoming of ubiquity: (1) feed viscosity is large, easily produces mildew, repeatedly need change feed, easily cause survival rate of larvae while wasting time and energy, percentage of pupation declines, and abnormal pupa rate is high.(2) nutrition is balanced not, the easy abnormal wing of adult, and raises how to have degeneration for rear population.(3) use hand operated mixing forage mixture, not only require great effort and stir uneven, thus nutritional labeling can not fully be mixed, larva growing way is uneven.In addition, for the rearing method of beet armyworm, Huang Chunxia etc. raise beet armyworm (Huang Chunxia etc., 2002) with supporting worm cylinder; And Mu Wei etc. use flat bottom glass pipe, 1-3 group support in age, then two first tube feed are supported to pupating (Mu Wei etc., 2002); Xu Jinhan etc. use water spinach leaf to raise 1-3 instar larvae (Xu Jinhan etc., 1998); The uses such as Wang Wei 24 orifice plate group support 1-3 instar larvae, then uses flat bottom glass Guan Yiguan two to raise (Wang Wei etc., 2011).Above-mentioned method for breeding has following shortcoming: it is air-dry that (1) causes feed to be easy to foster worm cylinder and the raising of flat glass tube, repeatedly need change feed, and flat bottom glass Guan Yiguan can only feed two, after being finished scrub and sterilizing works also quite huge, to take a lot of work so time-consuming effort, be not suitable for extensive raising.(2) because beet armyworm raising temperature is higher, and humidity is quite large on beet armyworm impact, use water spinach leaf to raise delicacies of the season leaf and be easy to languescent water outlet, thus drowned larva, if and put into a large amount of blade wherein, humidity can be caused excessive because of crossing multiple-blade wound again in insect box, except drowning easily larva, excessive humidity also easily can cause bacterium mould virus infections.
Summary of the invention
The present invention is directed to the deficiency that above-mentioned field exists, the man-made feeds that a kind of novel suitable beet armyworm is raised are provided, the preparation method of these man-made feeds be provided simultaneously and raise the method for beet armyworm, thus realizing extensive, long-term, the continuously rearing in standard worm source.
A kind of Artificial Diets For Beet Armyworm, is made up of following composition and weight percentage thereof:
Wheat bran: 3.5%-5%; Ash dish powder: 2.5%-3%; Corn flour: 1.0%-1.6%; Casein: 2.0%-3.0%; Ripe soy meal: 2.5%-3.5%; Dusty yeast: 1.5%-2.5%; Agar powder: 1.0%-1.8%; Distilled water: 75%-83%; Cholesterol: 0.06%-0.08%; Soybean edible oil: 0.2%-0.5%; Ascorbic acid: 0.4%-0.7%; Methyl p-hydroxybenzoate: 0.2%-0.3%; Sorbic acid: 0.05%-0.15%.
As preferably, every part of feed comprises: wheat bran: 4.05%-4.2%; Ash dish powder: 2.7%-2.8%; Corn flour: 1.3%-1.5%; Casein: 2.7%-2.8%; Ripe soy meal: 2.7%-2.8%; Dusty yeast: 2.0%-2.3%; Agar powder: 1.4%-1.5%; Distilled water: 80%-83%; Cholesterol: 0.06%-0.07%; Soybean edible oil: 0.25%-0.30%; Ascorbic acid: 0.5%-0.6%; Methyl p-hydroxybenzoate: 0.23%-0.25%; Sorbic acid: 0.10%-0.12%.
I.e. wheat bran: 30g; Ash dish powder: 20g; Corn flour: 10g; Casein: 20g; Ripe soy meal: 20g; Dusty yeast: 15g; Agar powder: 11g; Distilled water: 600ml; Cholesterol: 0.45g; Soybean edible oil: 2.1ml; Ascorbic acid: 4.2g (0.56%); Methyl p-hydroxybenzoate: 1.75g; Sorbic acid: 0.84g.
The preparation method of above-mentioned Artificial Diets For Beet Armyworm, carries out according to the following steps:
(1) preparation of ripe soy meal: buy soya bean from the market and toast 2h in 120 DEG C of baking ovens, then grind in beveller, then ground analysis for soybean powder to be put in kraft paper bag sterilizing 1.5h in high-temperature heat sterilization pot, after taking the dish out of the pot, soya-bean cake is pulverized, then evenly spread out after drying and place back in kraft paper bag, putting on label, to be placed on 5 DEG C of Refrigerator stores for subsequent use;
(2) preparation of grey dish powder: fresh goosefoot leaf is cleaned, dry or naturally dry under being placed in sunlight, then pulverized 90 mesh sieves, and got sieve inferior lobe powder and load and be placed in 120 DEG C of baking ovens in kraft paper bag and toast 0.5h, putting on label, to be placed on 5 DEG C of Refrigerator stores for subsequent use;
(3) processing of wheat bran: ground in beveller by the wheat bran that market is buied, is loaded in kraft paper bag and is placed in 120 DEG C of baking ovens simultaneously and toasts 0.5h, and putting on label, to be placed on 5 DEG C of Refrigerator stores for subsequent use;
(4) be loaded in kraft paper bag by commercially available corn flour and be placed in 120 DEG C of baking ovens and toast 0.5h, putting on label, to be placed on 5 DEG C of Refrigerator stores for subsequent use;
(5) dusty yeast is commercially available low sugar Angel Yeast, is placed in 5 DEG C of Refrigerator stores for subsequent use without the need to sterilizing;
(6) take ripe soy meal, grey dish powder, wheat bran, dusty yeast, corn flour and casein respectively in above feed ratio, be then put in homogenizer;
(7) take agar powder and be placed in pot, pour in pot by the distilled water measured, slowly rock pot until agar dissolves, then boiled by aqueous agar solution, period constantly stirs, and after seething with excitement again after infusion 5min, is transferred in homogenizer;
(8) by adherent for homogenizer powder stirring and dissolving, after all mixtures stir, add soybean edible oil, again stir;
(9) fume hood is opened make to add the cholesterol, ascorbic acid, methyl p-hydroxybenzoate and the sorbic acid that take after below mixture fast cooling to 60 DEG C, preprepared is poured in the porcelain dish of high-temperature sterilization after stirring, after its cooled and solidified, be cut into 2-3mm thin slice by knife and be placed in 5 DEG C of refrigerators and save backup.
Feed is in enormous quantities, the method for continuously rearing beet armyworm, carries out according to the following steps:
(1) ovum-newly hatched larvae: have the ovum paper scissors of Beet Armyworm Eggs to be cut into the little scraps of paper product and be placed in commercially available 90mm × 15mm aseptic plastic culture dish, after ovum blackening, culture dish is placed in the arbitrary man-made feeds thin slice of tweezers gripping two pieces of 20mm × 20mm × 2mm claims 1 to 3, and note being sure not feed to be pressed on pieces of an egg, put 6 layers of 100mm × 150mm toilet paper on culture dish, cover culture dish lid, wait for that larva hatches completely and abandon ovum paper;
(2) 1-3 instar larvaes: 1-3 instar larvaes are raised always in culture dish, larva 200-300 head in each culture dish, places two pieces of arbitrary man-made feeds thin slices of 40mm × 20mm × 2mm claims 1 to 3 in each culture dish;
(3) 3 instar larvaes-pupate: grow to after 3 ages until newly hatched larvae, being divided by larva with writing brush is allocated in other culture dish, each culture dish 10-17 head, two pieces of arbitrary man-made feeds thin slices of 40mm × 20mm × 2mm claims 1 to 3 are placed in each culture dish, wait that growing to for 4 age Mos removes excreta, adds a block length 30mm × 20mm × arbitrary man-made feeds of 2mm claims 1 to 3, and changes a toilet paper, support to during prepupal period just feed eaten up, and let alone to pupate in culture dish;
(4) pupate-sprout wings: after pupating 3-4 days with little spoon pupa drawn out distinguish male and female after be placed in 100mm × 25mm glass culture dish that bottom is placed with filter paper respectively, then the upper 90mm × 21cm glass paper cap of cover, then cover on top in order to ventilate with nylon gauze, then wait for that it is sprouted wings;
(5) sprout wings-lay eggs: dustbin with holes for family expenses is put polybag as mating cage, at bottom layer overlay filter paper or newspaper, inside put 10% honey as food; Use 190mm × 110mm 80g brown paper as ovum card, brown paper needs folding 10-15 to roll over before the use, by sprout wings after adult according to male and female 2:1 ratio totally 100 adults put into mating cage, mating put into ovum card after one day, change ovum card two days later, within every two days later, change a deutovum card, and change food every day;
Environmental condition in described insectary is temperature is 27 ± 1 DEG C, and indoor relative humidity is 40%-50%, and the photoperiod is L14: D10.
Need irradiate 2h under uviol lamp after wherein a generation is often raised in insectary, and ventilation is windowed once in insectary by every 3 days.
200-300 grain ovum is placed in each culture dish in described step (1).
50 pupas are put at most in each culture dish in described step (4).
Before wherein supporting worm, support worm little writing brush used, tweezers, scissors, little spoon etc. will through high-temperature heat sterilizations, the softness and little writing brush hairbrush used will be tried one's best.
This feed beneficial effect of the invention is:
(1) this feed raw materials is all easy to obtain, make grey dish powder grey dish used to be seen everywhere in the wild, can dry and pulverize and sieve for subsequent use with plant pulverizer tapping in a large number the 5-10 month grey dish, manufacture craft is simple, and cost is lower, the shelf-life longer (6 months), and grey dish can reduce feed viscosity, improve survival rate of larvae, percentage of pupation, reduce abnormal pupa rate, and the various vitamins needed for larvae development can be provided;
(2) use homemade ripe soy meal in this feed, it with on market the advantage of selling compared with soy meal be that, containing a large amount of oil content, it provides the linoleic acid needed for insect wing development together with edible oil, improve eclosion rate, reduce abnormal wing rate;
(3) in this feed, cholesterol used contributes to the hatching of ovum, improves incubation rate;
(4) use homogenizer in feed making process, without the need to hand operated mixing, labour-saving enables nutritional labeling mix simultaneously, makes larva growing way neat;
(5) the average pupa of this diet beet armyworm out weighs 111.8mg, and average moth weighs 100.4mg, and survival rate of larvae reaches 91%, eclosion rate 98%, and the vitality of pupa is comparatively strong, can store and reach 7 days in 5 DEG C of refrigerators.
This method for breeding beneficial effect of the invention is:
(1) use commercially available 90mm × 15mm aseptic plastic culture dish in the present invention in larva raising process always, buy and can use, without the need to sterilizing, and cheap, cost is lower;
(2) in the present invention, larva grows to without the need to single head picking after 3 ages, only need divide with writing brush and be allocated in other culture dishes, and can breeding grub 17 more than in each culture dish, greatly reduces labour and feeding time, realizes extensive batch and raise;
(3) toilet paper is used to cover on culture dish in the present invention, the benefit of toilet paper is that it can absorb moisture unnecessary in feeding environment, larva is made to exempt from because of the excessive drowned danger of humidity, and can make conversely humidity in feeding environment remain on larva be applicable to grow scope, indoor without the need to humidifier in feeding process, simultaneously suitable humidity can make feed not easily air-dry, 3 instar larvaes-period of pupating only need add a feed, and not easily fungal infection, time saving and energy saving province feed, toilet paper is also for larvae pupation provides space in addition;
(4) use repeatedly folding 190mm × 110mm 80g brown paper as ovum card, cater to beet armyworm and assemble the characteristic of laying eggs, beet armyworm prefers to lay eggs at backlight folding line place, and egg laying amount is more.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail.
Embodiment 1 (preparation 735g man-made feeds)
Wheat bran: 30g; Ash dish powder: 20g; Corn flour: 10g; Casein: 20g; Ripe soy meal: 20g; Dusty yeast: 15g; Agar powder: 11g; Distilled water: 600ml; Cholesterol: 0.45g; Soybean edible oil: 2.1ml; Ascorbic acid: 4.2g; Methyl p-hydroxybenzoate: 1.75g; Sorbic acid: 0.84g.
Embodiment 2 feed formulation method:
In implementation process, homogenizer is soaked in the chlorine water of 400mg/L the 20min that sterilizes, in case feed sense bacterium in preparation process.
(1) preparation of ripe soy meal: buy good quality soybeans from the market and toast 2h in 120 DEG C of baking ovens, then grind in beveller, grind very thin as far as possible, then ground analysis for soybean powder is put in self-control kraft paper bag in high-temperature heat sterilization pot sterilizing 1.5h, after taking the dish out of the pot, the soya-bean cake gone out is pulverized on newspaper, then evenly spread out after drying and place back in kraft paper bag, putting on label, to be placed on 5 DEG C of Refrigerator stores for subsequent use;
(2) preparation of grey dish powder: fresh goosefoot leaf is cleaned, naturally dry in baking oven after drying in the sun, then pulverized 90 mesh sieves, and got sieve inferior lobe powder and load and be placed in 120 DEG C of baking ovens in kraft paper bag and toast 0.5h, putting on label, to be placed on 5 DEG C of Refrigerator stores for subsequent use;
(3) processing of wheat bran: ground in beveller by the wheat bran that market is buied, is loaded in kraft paper bag and is placed in 120 DEG C of baking ovens simultaneously and toasts 0.5h;
(4) be loaded in kraft paper bag by commercially available Corn bread and be placed in 120 DEG C of baking ovens and toast 0.5h, putting on label, to be placed on 5 DEG C of Refrigerator stores for subsequent use;
(5) dusty yeast is commercially available low sugar Angel Yeast, is placed in 5 DEG C of Refrigerator stores for subsequent use without the need to sterilizing;
(6) take ripe soy meal, grey dish powder, wheat bran, dusty yeast, Corn bread bits and casein respectively in Disposable paper cup in above feed ratio, be then put in homogenizer;
(7) take agar powder and be placed in pot, the distilled water measured is poured in pot, slowly rock pot until agar dissolves, open electromagnetic oven, be adjusted to maximum until agar boils, period constantly stirs, be adjusted to middle gear infusion 5min after seething with excitement after, be transferred in homogenizer;
(8) with chopsticks by adherent for homogenizer powder stirring and dissolving, open homogenizer power supply use low or first gear stir, after all mixtures stir, add soybean edible oil, again stir;
(9) fume hood is opened make to add the cholesterol, ascorbic acid, methyl p-hydroxybenzoate and the sorbic acid that take after below mixture fast cooling to 60 DEG C, preprepared is poured in the porcelain dish of high-temperature sterilization after stirring, after its cooled and solidified, be cut into 2-3mm thin slice by knife and be placed in 5 DEG C of refrigerators and save backup.
Extensive, long-term, the continuously rearing method of embodiment 3 beet armyworm
Environmental condition in the insectary of artificial feeding beet armyworm is temperature is 27 ± 1 DEG C, indoor relative humidity is 40%-50%, photoperiod is L14: D10, and after often raising a generation need insectary to irradiate 2h under uviol lamp, with the disinfectant spray disinfectant 20min of 400mg/L, and ventilation is windowed once in insectary by every 3 days.Also will note indoor clean and tidy clean in feeding process, the middle polypide as thoughts bacterium or infection virus will clean out insectary in time.Before foster worm, support worm little writing brush used, tweezers, scissors, little spoon etc. will through high-temperature heat sterilizations, the softness and little writing brush hairbrush used will be tried one's best.
(1) ovum-newly hatched larvae: have the ovum paper scissors of Beet Armyworm Eggs to be cut into the little scraps of paper product and be placed in commercially available 90mm × 15mm aseptic plastic culture dish, 200-300 grain ovum is placed in each culture dish, after ovum blackening, culture dish is placed in tweezers gripping two pieces of 20mm × 20mm × 2mm feed thin slice, and note being sure not feed to be pressed on pieces of an egg, put 6 layers of (i.e. commercially available 3 layers of toilet paper two segment doubling) 100mm × 150mm toilet paper on culture dish, cover culture dish lid, wait for that larva hatches completely and abandon ovum paper;
(2) 1-3 instar larvaes: 1-3 instar larvaes are raised always in culture dish, larva 200-300 head in each culture dish, places two pieces of 40mm × 20mm × 2mm feed thin slices in each culture dish;
(3) 3 instar larvaes-pupate: grow to after 3 ages until newly hatched larvae, being divided by larva with writing brush is allocated in other culture dish, each culture dish 10-17 head, two pieces of 40mm × 20mm × 2mm feed thin slices are placed in each culture dish, wait that growing to for 4 age Mos removes excreta, adds a block length 30mm × 20mm × 2mm feed, and changes a toilet paper, support to during prepupal period just feed eaten up, and let alone to pupate in culture dish;
(4) pupate-sprout wings: after pupating 3-4 days with little spoon pupa drawn out distinguish male and female after be placed in 100mm × 25mm glass culture dish that bottom is placed with filter paper respectively, then the upper 90mm × 21cm glass paper cap of cover, then covers on top in order to ventilate with nylon gauze.Put at most 50 pupas in each culture dish, then wait for that it is sprouted wings;
(5) sprout wings-lay eggs: dustbin with holes for family expenses is put polybag as mating cage, at bottom layer overlay filter paper or newspaper, inside put 10% honey as food.Use 190mm × 110mm 80g brown paper as ovum card, brown paper needs folding 10-15 to roll over before the use.By the adult after sprouting wings according to male and female 2:1 ratio totally 100 adults put into mating cage, mating put into ovum card after one day, changed ovum card two days later, within later every two days, changed a deutovum card, and changed food every day;
(6) continue, by above-mentioned steps (1), (2), (3), (4), (5) cycling continuously rearing, constantly to provide required worm source, terminate until raise.
Embodiment 4 man-made feeds compliance test result
Test material and method:
For examination worm source: the winter generation adult that worm source gathers from field, Qingdao of Shandong province, through the Beet Armyworm Eggs in indoors artificial diet 10 generation.
For examination feed: man-made feeds and preparation method thereof described in embodiment 1,2.
Test method: examination worm method for breeding is with reference to method described in embodiment 3, often process and repeat for 5 times, often repeat for examination larva 20, all record the survival rate of larvae of larva in feeding process, eclosion rate, larva go through the phase, pupa time, percentage of pupation, preoviposition period, 4d egg laying amount after emergence, sprout wings after 4d number of copulations, pupa heavy, the moth index such as heavily.This test and in February ,-2015 in October, 2014 complete in Plant Protection institute, Chinese Academy of Agricultral Sciences laboratory.
Data statistics:
Analysis of experimental data adopts SAS software (version is The SAS System for Windows V8), and in literary composition, data all represent with mean value ± standard error (Mean ± SE).
Result of the test:
The effect that beet armyworm raised by man-made feeds is as follows:
As can be seen from top: with this diet beet armyworm, the average pupa of beet armyworm weighs 111.8mg, and first emergence moth weighs 100.4 ㎎, and larva average viability is 91%, and eclosion rate is 98%, and after sprouting wings, 4d is average singly femalely lays eggs 729.8.In sum, this diet effect is better, reaches object that is long-term, extensive, continuously rearing beet armyworm.
Claims (8)
1. an Artificial Diets For Beet Armyworm, is made up of following composition and weight percentage thereof:
Wheat bran: 3.5%-5%; Ash dish powder: 2.5%-3%; Corn flour: 1.0%-1.6%; Casein: 2.0%-3.0%; Ripe soy meal: 2.5%-3.5%; Dusty yeast: 1.5%-2.5%; Agar powder: 1.0%-1.8%; Distilled water: 75%-83%; Cholesterol: 0.06%-0.08%; Soybean edible oil: 0.2%-0.5%; Ascorbic acid: 0.4%-0.7%; Methyl p-hydroxybenzoate: 0.2%-0.3%; Sorbic acid: 0.05%-0.15%.
2. Artificial Diets For Beet Armyworm according to claim 1, wherein wheat bran: 4.05%-4.2%; Ash dish powder: 2.7%-2.8%; Corn flour face: 1.3%-1.5%; Casein: 2.7%-2.8%; Ripe soy meal: 2.7%-2.8%; Dusty yeast: 2.0%-2.3%; Agar powder: 1.4%-1.5%; Distilled water: 80%-83%; Cholesterol: 0.06%-0.07%; Soybean edible oil: 0.25%-0.30%; Ascorbic acid: 0.5%-0.6%; Methyl p-hydroxybenzoate: 0.23%-0.25%; Sorbic acid: 0.10%-0.12%.
3. Artificial Diets For Beet Armyworm according to claim 1, wherein wheat bran: 30g; Ash dish powder: 20g; Corn flour: 10g; Casein: 20g; Ripe soy meal: 20g; Dusty yeast: 15g; Agar powder: 11g; Distilled water: 600ml; Cholesterol: 0.45g; Soybean edible oil: 2.1ml; Ascorbic acid: 4.2g; Methyl p-hydroxybenzoate: 1.75g; Sorbic acid: 0.84g.
4. the preparation method of Artificial Diets For Beet Armyworm, carries out according to the following steps:
(1) preparation of ripe soy meal: buy soya bean from the market and toast 2h in 120 DEG C of baking ovens, then grind in beveller, then ground analysis for soybean powder to be put in kraft paper bag sterilizing 1.5h in high-temperature heat sterilization pot, after taking the dish out of the pot, soya-bean cake is pulverized, then evenly spread out after drying and place back in kraft paper bag, putting on label, to be placed on 5 DEG C of Refrigerator stores for subsequent use;
(2) preparation of grey dish powder: fresh goosefoot leaf is cleaned, dry or naturally dry under being placed in sunlight, then pulverized 90 mesh sieves, and got sieve inferior lobe powder and load and be placed in 120 DEG C of baking ovens in kraft paper bag and toast 0.5h, putting on label, to be placed on 5 DEG C of Refrigerator stores for subsequent use;
(3) processing of wheat bran: ground in beveller by the wheat bran that market is buied, is loaded in kraft paper bag and is placed in 120 DEG C of baking ovens simultaneously and toasts 0.5h, and putting on label, to be placed on 5 DEG C of Refrigerator stores for subsequent use;
(4) be loaded in kraft paper bag by commercially available Corn bread and be placed in 120 DEG C of baking ovens and toast 0.5h, putting on label, to be placed on 5 DEG C of Refrigerator stores for subsequent use;
(5) dusty yeast is commercially available low sugar Angel Yeast, is placed in 5 DEG C of Refrigerator stores for subsequent use without the need to sterilizing;
(6) take ripe soy meal, grey dish powder, wheat bran, dusty yeast, corn flour and casein respectively in above feed ratio, be then put in homogenizer;
(7) take agar powder and be placed in pot, pour in pot by the distilled water measured, slowly rock pot until agar dissolves, then boiled by aqueous agar solution, period constantly stirs, and after seething with excitement again after infusion 5min, is transferred in homogenizer;
(8) by adherent for homogenizer powder stirring and dissolving, after all mixtures stir, add soybean edible oil, again stir;
(9) fume hood is opened make to add the cholesterol, ascorbic acid, methyl p-hydroxybenzoate and the sorbic acid that take after below mixture fast cooling to 60 DEG C, preprepared is poured in the porcelain dish of high-temperature sterilization after stirring, after its cooled and solidified, be cut into 2-3mm thin slice by knife and be placed in 5 DEG C of refrigerators and save backup.
5. the method for in enormous quantities, the continuously rearing beet armyworm of feed, carry out according to the following steps:
(1) ovum-newly hatched larvae: have the ovum paper scissors of Beet Armyworm Eggs to be cut into the little scraps of paper product and be placed in commercially available 90mm × 15mm aseptic plastic culture dish, after ovum blackening, culture dish is placed in the arbitrary man-made feeds thin slice of claims 1 to 3 that tweezers gripping two block size is 20mm × 20mm × 2mm, and note being sure not feed to be pressed on pieces of an egg, put 6 layers of 100mm × 150mm toilet paper on culture dish, cover culture dish lid, wait for that larva hatches completely and abandon ovum paper;
(2) 1-3 instar larvaes: 1-3 instar larvaes are raised always in culture dish, larva 200-300 head in each culture dish, places two pieces of arbitrary man-made feeds thin slices of 40mm × 20mm × 2mm claims 1 to 3 in each culture dish;
(3) 3 instar larvaes-pupate: grow to after 3 ages until newly hatched larvae, being divided by larva with writing brush is allocated in other culture dish, each culture dish 10-17 head, the arbitrary man-made feeds thin slice of claims 1 to 3 of two pieces of 40mm × 20mm × 2mm is placed in each culture dish, wait that growing to for 4 age Mos removes excreta, adds a block length 30mm × 20mm × arbitrary man-made feeds of 2mm claims 1 to 3, and changes a toilet paper, support to during prepupal period just feed eaten up, and let alone to pupate in culture dish;
(4) pupate-sprout wings: after pupating 3-4 days with little spoon pupa drawn out distinguish male and female after be placed in 100mm × 25mm glass culture dish that bottom is placed with filter paper respectively, then the upper 90mm × 21cm glass paper cap of cover, then cover on top in order to ventilate with nylon gauze, then wait for that it is sprouted wings;
(5) sprout wings-lay eggs: dustbin with holes for family expenses is put polybag as mating cage, at bottom layer overlay filter paper or newspaper, inside put 10% honey as food; Use 190mm × 110mm 80g brown paper as ovum card, brown paper needs folding 10-15 to roll over before the use, by sprout wings after adult according to male and female 2:1 ratio totally 100 adults put into mating cage, mating put into ovum card after one day, change ovum card two days later, within every two days later, change a deutovum card, and change food every day;
Environmental condition in described insectary is temperature is 27 ± 1 DEG C, and indoor relative humidity is 40%-50%, and the photoperiod is L14: D10.
6. method according to claim 5, need irradiate 2h after wherein a generation is often raised in insectary under uviol lamp, and ventilation is windowed once in insectary by every 3 days.
7. method according to claim 5, places 200-300 grain ovum in each culture dish in described step (1).
8. method according to claim 5, puts at most 50 pupas in each culture dish in described step (4).
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| CN105124220B (en) * | 2015-08-21 | 2020-01-17 | 福建省农业科学院植物保护研究所 | Semi-synthetic artificial feed for mango horizontal line tail noctuid larvae and preparation method thereof |
| CN105145495A (en) * | 2015-09-10 | 2015-12-16 | 延安大学 | Artificial breeding method for jujube tree porthesia similis |
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| CN105192263A (en) * | 2015-10-09 | 2015-12-30 | 刘新霞 | Armadillidium breeding fodder and preparation method thereof |
| CN106260794A (en) * | 2016-08-09 | 2017-01-04 | 金陵科技学院 | A kind of Prodenia litura man-made feeds and Prodenia litura indoors artificial method for breeding |
| CN106259221B (en) * | 2016-08-19 | 2019-08-27 | 华巧波 | A method of raising beet armyworm |
| CN106259221A (en) * | 2016-08-19 | 2017-01-04 | 华巧波 | A kind of method raising beet armyworm |
| CN106306389A (en) * | 2016-08-19 | 2017-01-11 | 华巧波 | Fodder for improving breeding efficiency of beet armyworm |
| CN106942157A (en) * | 2017-03-04 | 2017-07-14 | 云南农业大学 | A kind of Moringa Nao snout moth's larva method for breeding of use man-made feeds |
| CN106954765A (en) * | 2017-04-11 | 2017-07-18 | 广东人为峰健康管理有限公司 | A kind of insect feedstuff of green safety and its preparation method and application |
| CN110089642A (en) * | 2019-06-15 | 2019-08-06 | 安徽省农业科学院植物保护与农产品质量安全研究所 | A kind of Spodopterafrugiperda man-made feeds |
| CN111328935A (en) * | 2020-04-08 | 2020-06-26 | 四川省农业科学院植物保护研究所 | Feed for beet armyworm and preparation method and application thereof |
| CN111328935B (en) * | 2020-04-08 | 2022-12-23 | 四川省农业科学院植物保护研究所 | Feed for beet armyworm and preparation method and application thereof |
| CN116530621A (en) * | 2023-06-14 | 2023-08-04 | 烟台大学 | Mythimna separata feed and indoor mythimna separata feeding method |
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