CN104757333B - A kind of man-made feeds of beet armyworm, preparation and method for breeding - Google Patents
A kind of man-made feeds of beet armyworm, preparation and method for breeding Download PDFInfo
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Abstract
The present invention relates to a kind of man-made feeds of beet armyworm, preparation and method for breeding belong to insect feedstuff field.Artificial Diets For Beet Armyworm is made of following composition：Wheat bran, grey vegetable powder, maize flour, casein, ripe soy meal, yeast powder, agar powder, distilled water, cholesterol, soybean edible oil, ascorbic acid, methyl p-hydroxybenzoate and sorbic acid.This feed raw materials are easy to obtain, and manufacture craft is simple, and cost is relatively low, shelf-life is longer (6 months), and grey dish can reduce feed viscosity, improve survival rate of larvae, percentage of pupation, reduce abnormal pupa rate, and the beet armyworm that the various vitamins needed for larvae development the raise out pupa that is averaged can be provided and weighed 111.8mg, average moth weighs 100.4mg, and survival rate of larvae is up to 91%, eclosion rate 98%, and the vitality of pupa is stronger, can store and be up to 7 days in 5 DEG C of refrigerators.This feed feeding effect is preferable, has achieved the purpose that long-term, extensive, continuously rearing.
Description
Technical field
The present invention relates to insect feedstuff and breeding technologies, and in particular to the man-made feeds of beet armyworm, preparation method and
Using the method for breeding of the feed.
Background technology
Beet armyworm spodoptera exiguaBelong to Lepidoptera Lepidoptera, Noctuidae Noctuidae,
It is that a kind of feeding habits are miscellaneous, the strong pest of drug resistance often causes to seriously threaten to agricultural production and economy.In China, 80 years 20th century
Mainly cause harm the ground such as Beijing, Hebei, Henan, Shandong and Shaanxi for preceding beet armyworm, though northeast, northwest and the Yangtze river basin have point
Cloth, but cause harm and do not weigh.Since the middle and later periods eighties, beet armyworm area of causing harm is gradually expanded, and the range to cause harm is increasing, makes
At economic loss it is also increasingly severe (Yu You is gloomy etc., 1989；Jiang Xingfu, 1998；Song Guochun, 1998；Vehicle Shanxi Yunnan etc., 2001；
Zhang Zhen woods, 2004；Song Tianjun etc., 2004；Cao Chuntian etc., 2005；Tan Xiufang etc., 2005；Explain jump peak etc., 2007).
And the efficient medicament of beet armyworm and recipe determination, resistance risk assessment, natural enemy and virus expand numerous, biological control and
The element task of the multinomial research such as science of heredity and Physiology and biochemistry be required for large batch of normal development, neat and consistent beet armyworm
Larva.The development to beet exigua larvae man-made feeds and its method for breeding have been carried out a large amount of research both at home and abroad at present.It is public
Though the man-made feeds effect for opening report is preferable, the following disadvantage of generally existing：(1) feed viscosity is big, is also easy to produce mildew, needs multiple
Feed is replaced, survival rate of larvae is easily led to while time-consuming and laborious, percentage of pupation declines, and abnormal pupa rate is high.(2) nutrition is not balanced enough,
The easily abnormal wing of adult, and raising mostly has degeneration for rear population.(3) hand operated mixing forage mixture is used, not only laborious and stirring is not
Uniformly, to prevent nutritional ingredient from mixing well, larva growing way is irregular.In addition, the artificial feeding for beet armyworm
Method, yellow spring rosy clouds etc. raise beet armyworm (yellow spring rosy clouds etc., 2002) with worm cylinder is supported；And Mu Wei etc. uses flat bottom glass pipe, 1-3 ages
Group rearing, then two first tube feed support to pupate (Mu Wei etc., 2002)；Xu Jinhan etc. uses hollow dish leaf raising 1-3 instar larvae (Xu
Jin Han etc., 1998)；Wang Wei etc. uses 24 orifice plate group rearing 1-3 instar larvaes, then the both ends flat bottom glass Guan Yiguan is used to raise
(Wang Wei etc., 2011).Above-mentioned method for breeding has the disadvantage that：(1) feed is caused to hold very much with foster worm cylinder and the raising of flat glass tube
It easily air-dries, need to repeatedly replace feed, and flat bottom glass Guan Yiguan can only feed both ends, scrubbing after being finished and sterilizing works
It is quite huge, thus it is time-consuming laborious, it is not suitable for raising on a large scale.(2) because beet armyworm raising temperature is higher, and humidity
On beet armyworm influence it is quite big, dish leaf is easy to languescent water outlet when raise using hollow dish leaf, to be drowned larva, and if
A large amount of blades are wherein put into, humidity can be caused excessive because of multiple-blade wound is crossed again in insect box, other than drowning easily larva,
Excessive humidity can also be easy to cause the infection of bacterium mould virus.
Invention content
The present invention provides a kind of artificial feeding of novel suitable beet armyworm raising for deficiency existing for above-mentioned field
Material, at the same provide the man-made feeds preparation method and raise beet armyworm method, to realize standard worm sources it is extensive,
For a long time, continuously rearing.
A kind of Artificial Diets For Beet Armyworm is made of following composition and its weight percentage：
Wheat bran：3.5%-5%；Grey vegetable powder：2.5%-3%；Maize flour：1.0%-1.6%；Casein：2.0%-
3.0%；Ripe soy meal：2.5%-3.5%；Yeast powder：1.5%-2.5%；Agar powder：1.0%-1.8%；Distilled water：75%-
83%；Cholesterol：0.06%-0.08%；Soybean edible oil：0.2%-0.5%；Ascorbic acid：0.4%-0.7%；To hydroxyl
Methyl benzoate：0.2%-0.3%；Sorbic acid：0.05%-0.15%.
Preferably, every part of feed includes：Wheat bran：4.05%-4.2%；Grey vegetable powder：2.7%-2.8%；Maize flour：
1.3%-1.5%；Casein：2.7%-2.8%；Ripe soy meal：2.7%-2.8%；Yeast powder：2.0%-2.3%；Agar
Powder：1.4%-1.5%；Distilled water：80%-83%；Cholesterol：0.06%-0.07%；Soybean edible oil：0.25%-
0.30%；Ascorbic acid：0.5%-0.6%；Methyl p-hydroxybenzoate：0.23%-0.25%；Sorbic acid：0.10%-
0.12%.
That is wheat bran：30g；Grey vegetable powder：20g；Maize flour：10g；Casein：20g；Ripe soy meal：20g；Yeast powder：15g；
Agar powder：11g；Distilled water：600ml；Cholesterol：0.45g；Soybean edible oil：2.1ml；Ascorbic acid：4.2g (0.56%)；
Methyl p-hydroxybenzoate：1.75g；Sorbic acid：0.84g.
The preparation method of above-mentioned Artificial Diets For Beet Armyworm carries out according to the following steps：
(1) preparation of ripe soy meal：Soya bean is bought from the market and toasts 2h in 120 DEG C of baking ovens, then in beveller
It grinds, ground analysis for soybean powder is then put in kraft paper bag in high-temperature heat sterilization pot the 1.5h that sterilizes, after taking the dish out of the pot
Soya-bean cake is pulverized, then uniformly spreads out and places back in kraft paper bag after drying, label is put on and is placed on 5 DEG C of refrigerators and save backup；
(2) preparation of grey vegetable powder：Fresh goosefoot leaf is cleaned, dries or is placed in naturally dry under sunlight, then crushed
90 mesh sieve, and take sieve inferior lobe powder to be fitted into kraft paper bag to be placed in 120 DEG C of baking ovens and toast 0.5h, put on label and be placed on 5 DEG C of refrigerators
It saves backup；
(3) processing of wheat bran：Commercially available wheat bran is ground in beveller, is loaded in kraft paper bag simultaneously
It is placed in 120 DEG C of baking ovens and toasts 0.5h, put on label and be placed on 5 DEG C of refrigerators and save backup；
(4) commercially available maize flour is loaded in kraft paper bag to be placed in 120 DEG C of baking ovens and toasts 0.5h, put on label and be placed on 5
DEG C refrigerator saves backup；
(5) yeast powder is commercially available low sugar Angel Yeast, and being placed in 5 DEG C of refrigerators without sterilizing saves backup；
(6) ripe soy meal, grey vegetable powder, wheat bran, yeast powder, maize flour and casein are weighed respectively in the above feed ratio,
Then it is put in homogenizer；
(7) weigh agar powder to be placed in pot, the distilled water of measurement poured into pot, slowly rock pot until agar dissolve,
Aqueous agar solution is boiled again, is during which stirred continuously, is transferred in homogenizer after infusion 5min again after boiling；
(8) by the adherent powder stirring and dissolving of homogenizer, wait for that all mixtures are stirring evenly and then adding into soybean edible oil, again
It stirs evenly；
(9) make mixture fast cooling that cholesterol, the Vitamin C weighed be added afterwards to 60 DEG C or less draught cupboard opening
Acid, methyl p-hydroxybenzoate and sorbic acid, pour into porcelain dish of the preprepared through high-temperature sterilization after stirring evenly, wait for it
After cooled and solidified, it is cut into 2-3mm thin slices by knife and is placed in 5 DEG C of refrigerators and save backup.
Feed high-volume, the method for continuously rearing beet armyworm, carry out according to the following steps：
(1) ovum-newly hatched larvae：There is the ovum paper of Beet Armyworm Eggs to be cut into the small scraps of paper with scissors production and is placed on commercially available 90mm
In × 15mm aseptic plastic culture dishes, after ovum blackening, it is thin to grip two pieces of above-mentioned man-made feeds of 20mm × 20mm × 2mm with tweezers
Piece is placed in culture dish, and pays attention to being sure not feed being pressed on pieces of an egg, sets 6 layers of 100mm × 150mm toilet papers in culture dish
On, culture ware lid is covered, ovum paper is abandoned after waiting for larva to hatch completely；
(2) 1-3 instar larvaes：1-3 instar larvaes are raised in culture dish always, larva 200-300 heads in each culture dish,
The above-mentioned man-made feeds thin slices of two pieces of 40mm × 20mm × 2mm are placed in each culture dish；
(3) 3 instar larvaes-pupate：It grows to 3 ages after newly hatched larvae, is allocated larva into other culture dishes with writing brush,
Each culture dish 10-17 heads are placed the above-mentioned man-made feeds thin slices of two pieces of 40mm × 20mm × 2mm in each culture dish, are waited for long to 4
Age end removes excreta, adds a block length 30mm × 20mm × above-mentioned man-made feeds of 2mm, and replace a toilet paper, supports to pre-
Just feed is eaten up when pupa time, and is allowed to pupate in culture dish；
(4) it pupates-sprouts wings：With small spoon pupa dug out after pupating 3-4 days and is respectively placed in bottom after distinguishing male and female and is placed with filter
In 100mm × 25mm glass culture dish of the scraps of paper, upper 90mm × 21cm glass paper caps are then covered, then cover on top with nylon gauze
Then portion waits for its emergence to divulge information；
(5) emergence-oviposition：Household dustbin with holes is put on into polybag as mating cage, in bottom layer overlay filter paper
Or newspaper, 10% honey is inside put as food；Using 190mm × 110mm 80g brown paper as ovum card, brown paper is using
It needs to fold 10-15 foldings before, by the adult after emergence according to male and female 2:Totally 100 adults are put into mating cage 1 ratio, mating
It is put into ovum card after one day, replaces ovum card two days later, replaces deutovum card, and replaces food daily within every two days later；
Environmental condition in the insectary is that temperature is 27 ± 1 DEG C, indoor relative humidity 40%-50%, the photoperiod
It is L14: D10.
Wherein insectary need to irradiate 2h after often raising a generation under ultraviolet lamp, and every 3 days primary by insectary windowing ventilation.
200-300 ovum are placed in the step (1) in each culture dish.
In the step (4) 50 pupas are at most put in each culture dish.
Before wherein supporting worm, small writing brush used in worm is supported, tweezers, scissors, small spoon etc. will be through high-temperature heat sterilizations, and small Mao used
Pen hair brush is soft as possible.
This feed advantageous effect of the invention is：
(1) this feed raw materials are easy to obtain, and make grey dish used in grey vegetable powder and are seen everywhere in the wild, can be in 5-
It largely taps grey dish October and dries and pulverized and sieved with plant pulverizer spare, manufacture craft is simple, and cost is relatively low, and the shelf-life is longer
(6 months), and grey dish can reduce feed viscosity, improve survival rate of larvae, percentage of pupation, reduce abnormal pupa rate, and can provide larva
Various vitamins needed for development；
(2) homemade ripe soy meal is used in this feed, the advantages of it is compared with sold soy meal in the market is
Containing a large amount of oils, it provides the linoleic acid needed for insect wing development together with edible oil, improves eclosion rate, reduces abnormal wing rate；
(3) cholesterol used contributes to the hatching of ovum in this feed, improves hatching rate；
(4) homogenizer is used during feed making, is not necessarily to hand operated mixing, so that nutritional ingredient is mixed while laborsaving
Uniformly, make larva growing way neat；
(5) beet armyworm that this feed the raises out pupa that is averaged weighs 111.8mg, and average moth weighs 100.4mg, and larva is deposited
Motility rate is up to 91%, eclosion rate 98%, and the vitality of pupa is stronger, can store and be up to 7 days in 5 DEG C of refrigerators.
This method for breeding advantageous effect of the invention is：
(1) it uses commercially available 90mm × 15mm aseptic plastics culture dish in the present invention during larva raising always, buys i.e.
It can be used, without sterilizing, and it is cheap, cost is relatively low；
(2) it is not necessarily to single head picking after larva grew to 3 ages in the present invention, need to be only allocated with writing brush into other culture dishes, and
In each culture dish can as many as breeding grub 17, greatly reduce labour and feeding time, realize that extensive batch is raised；
(3) use hygienic paper cap on culture dish in the present invention, toilet paper is advantageous in that it can be absorbed in feeding environment
Extra moisture makes larva from because of the excessive drowned danger of humidity, and humidity in feeding environment can be made to be maintained at children in turn
Worm is suitble to the range of growth, indoor in feeding process to be not necessarily to humidifier, while suitable humidity can make feed be not easy to air-dry, and 3
Instar larvae-only need to add a feed during pupating, and be not easy fungal infection, and time saving and energy saving province's feed is in addition hygienic
Paper also provides space for larvae pupation；
(4) it uses the 190mm × 110mm 80g brown paper repeatedly folded as ovum card, caters to beet armyworm aggregation oviposition
Characteristic, beet armyworm prefers to lay eggs at backlight folding line, and egg laying amount is more.
Specific implementation mode
With reference to embodiment, the present invention is described in further detail.
Embodiment 1 (prepares 735g man-made feeds)
Wheat bran：30g；Grey vegetable powder：20g；Corn flour：10g；Casein：20g；Ripe soy meal：20g；Yeast powder：15g；Fine jade
Cosmetics：11g；Distilled water：600ml；Cholesterol：0.45g；Soybean edible oil：2.1ml；Ascorbic acid：4.2g；Para hydroxybenzene first
Sour methyl esters：1.75g；Sorbic acid：0.84g.
2 feed formulation method of embodiment:
In implementation process, homogenizer is soaked in the chlorine water of 400mg/L and sterilizes 20min, to prevent being raised in preparation process
Material sense bacterium.
(1) preparation of ripe soy meal：Good quality soybeans are bought from the market and toast 2h in 120 DEG C of baking ovens, then in grinding
Ground in instrument, grind as possible it is finely ground, then by ground analysis for soybean powder be put in self-control kraft paper bag in it is damp and hot in high temperature
Sterilize 1.5h in autoclave, pulverizes the soya-bean cake to have gone out on newspaper after taking the dish out of the pot, and then uniformly spreads out and places back in ox after drying
Mulberry paper bag, puts on label and is placed on 5 DEG C of refrigerators and save backup；
(2) preparation of grey vegetable powder：Fresh goosefoot leaf is cleaned, is dried in baking oven after natural drying in the sun, then powder
90 mesh of broken mistake sieves, and takes sieve inferior lobe powder to be fitted into kraft paper bag to be placed in 120 DEG C of baking ovens and toast 0.5h, puts on label and be placed on 5 DEG C
Refrigerator saves backup；
(3) processing of wheat bran：Commercially available wheat bran is ground in beveller, is loaded in kraft paper bag simultaneously
It is placed in 120 DEG C of baking ovens and toasts 0.5h；
(4) commercially available Corn bread is loaded in kraft paper bag to be placed in 120 DEG C of baking ovens and toasts 0.5h, put on label and be placed on
5 DEG C of refrigerators save backup；
(5) yeast powder is commercially available low sugar Angel Yeast, and being placed in 5 DEG C of refrigerators without sterilizing saves backup；
(6) ripe soy meal, grey vegetable powder, wheat bran, yeast powder, Corn bread bits and cheese are weighed respectively in the above feed ratio
Then element is put in homogenizer in disposable paper cup；
(7) weigh agar powder to be placed in pot, the distilled water of measurement poured into pot, slowly rock pot until agar dissolve,
Open electromagnetic oven, be adjusted to maximum until agar boil, be during which stirred continuously, after middle gear infusion 5min is adjusted to after boiling,
It is transferred in homogenizer；
(8) the adherent powder stirring and dissolving of homogenizer is opened homogenizer power supply and is stirred using low or first gear, waited for chopsticks
All mixtures are stirring evenly and then adding into soybean edible oil, are again stirring for uniformly；
(9) make mixture fast cooling that cholesterol, the Vitamin C weighed be added afterwards to 60 DEG C or less draught cupboard opening
Acid, methyl p-hydroxybenzoate and sorbic acid, pour into porcelain dish of the preprepared through high-temperature sterilization after stirring evenly, wait for it
After cooled and solidified, it is cut into 2-3mm thin slices by knife and is placed in 5 DEG C of refrigerators and save backup.
Extensive, long-term, the continuously rearing method of 3 beet armyworm of embodiment
Environmental condition in the insectary of artificial feeding beet armyworm is that temperature is 27 ± 1 DEG C, and indoor relative humidity is
40%-50%, photoperiod L14: D10, and need insectary irradiating 2h under ultraviolet lamp after often raising a generation, use 400mg/L
Disinfectant spray disinfectant 20min, and every 3 days are primary by insectary windowing ventilation.It is in feeding process it is also noted that indoor clean and tidy
Totally, the intermediate polypide such as thoughts bacterium or virus infection will clean out insectary in time.Before supporting worm, small writing brush used in worm is supported,
Tweezers, scissors, small spoon etc. will be through high-temperature heat sterilizations, and small writing brush hairbrush used is soft as possible.
(1) ovum-newly hatched larvae：There is the ovum paper of Beet Armyworm Eggs to be cut into the small scraps of paper with scissors production and is placed on commercially available 90mm
In × 15mm aseptic plastic culture dishes, 200-300 ovum are placed in each culture dish, and after ovum blackening, two pieces are gripped with tweezers
20mm × 20mm × 2mm feed thin slices are placed in culture dish, and pay attention to be sure not feed being pressed on pieces of an egg, set 6 layers it is (i.e. commercially available
3 layers of two segment doubling of toilet paper) 100mm × 150mm toilet papers on culture dish, covering culture ware lid, wait for larva to hatch
Ovum paper is abandoned after complete；
(2) 1-3 instar larvaes：1-3 instar larvaes are raised in culture dish always, larva 200-300 heads in each culture dish,
Two pieces of 40mm × 20mm × 2mm feed thin slices are placed in each culture dish；
(3) 3 instar larvaes-pupate：It grows to 3 ages after newly hatched larvae, is allocated larva into other culture dishes with writing brush,
Each culture dish 10-17 heads place two pieces of 40mm × 20mm × 2mm feed thin slices in each culture dish, wait for long removing to 4 age ends
Excreta adds a block length 30mm × 20mm × 2mm feeds, and replaces a toilet paper, supports to just feed quilt when prepupal period
It eats up, and is allowed to pupate in culture dish；
(4) it pupates-sprouts wings：With small spoon pupa dug out after pupating 3-4 days and is respectively placed in bottom after distinguishing male and female and is placed with filter
In 100mm × 25mm glass culture dish of the scraps of paper, upper 90mm × 21cm glass paper caps are then covered, then cover on top with nylon gauze
Portion is divulging information.50 pupas are at most put in each culture dish, then wait for its emergence；
(5) emergence-oviposition：Household dustbin with holes is put on into polybag as mating cage, in bottom layer overlay filter paper
Or newspaper, 10% honey is inside put as food.Using 190mm × 110mm 80g brown paper as ovum card, brown paper is using
It needs to fold 10-15 foldings before.By the adult after emergence according to male and female 2:Totally 100 adults are put into mating cage 1 ratio, mating
It is put into ovum card after one day, replaces ovum card two days later, replaces deutovum card, and replaces food daily within every two days later；
(6) continue, by above-mentioned steps (1), (2), (3), (4), (5) circulate operation continuously rearing, constantly to provide required worm
Source, until raising terminates.
4 man-made feeds compliance test result of embodiment
Test material and method：
Selected insect source：The winter generation adult that worm sources are acquired from Qingdao of Shandong province field is raised through indoors artificial feed
The Beet Armyworm Eggs in 10 generations.
For trying feed：Embodiment 1,2 described man-made feeds and preparation method thereof.
Test method：Test worm method for breeding often handles 5 repetitions with reference to 3 the method for embodiment, often repeats for trying larva
20, the survival rate of larvae of larva, eclosion rate, larva are recorded in feeding process and goes through phase, pupa time, percentage of pupation, preoviposition period, plumage
4d number of copulations after 4d egg laying amounts, emergence, pupa weight, the moth indexs such as again after change.This experiment and 2 months -2015 years in October, 2014 exist
It is completed in Plant Protection institute, Chinese Academy of Agricultral Sciences laboratory.
Data statistics：
Analysis of experimental data uses SAS softwares (version is The SAS System for Windows V8), data in text
Indicated with average value ± standard error (Mean ± SE).
Test result：
The effect that man-made feeds raise beet armyworm is as follows：
From top it can be seen that：Beet armyworm is raised with the feed, and the beet armyworm pupa that be averaged weighs 111.8mg, just emergence
Moth weighs 100.4 ㎎, and larva average viability is 91%, eclosion rate 98%, the average single female ovipositions 729.8 of 4d after emergence.
In conclusion this feed feeding effect is preferable, long-term, extensive, continuously rearing beet armyworm is achieved the purpose that.
Claims (6)
1. a kind of Artificial Diets For Beet Armyworm is made of following composition：
Wheat bran：30g；Grey vegetable powder：20g；Maize flour：10g；Casein：20g；Ripe soy meal：20g；Yeast powder：15g；Agar powder：
11g；Distilled water：600ml；Cholesterol：0.45g；Soybean edible oil：2.1ml；Ascorbic acid：4.2g；P-hydroxybenzoic acid first
Ester：1.75g；Sorbic acid：0.84g.
2. the preparation method of Artificial Diets For Beet Armyworm described in claim 1 carries out according to the following steps：
(1) preparation of ripe soy meal：Soya bean is bought from the market and toasts 2h in 120 DEG C of baking ovens, is then ground in beveller
It crushes, ground analysis for soybean powder is then put in kraft paper bag in high-temperature heat sterilization pot the 1.5h that sterilizes, by beans after taking the dish out of the pot
Cake pulverizes, and then uniformly spreads out and places back in kraft paper bag after drying, and puts on label and is placed on 5 DEG C of refrigerators and saves backup；
(2) preparation of grey vegetable powder：Fresh goosefoot leaf is cleaned, naturally dry under sunlight is dried or be placed in, then crushed 90 mesh
Sieve takes sieve inferior lobe powder to be fitted into kraft paper bag to be placed in 120 DEG C of baking ovens and toast 0.5h, puts on label and is placed on 5 DEG C of refrigerators preservations
It is spare；
(3) processing of wheat bran：Commercially available wheat bran is ground in beveller, loaded on being placed in simultaneously in kraft paper bag
0.5h is toasted in 120 DEG C of baking ovens, label is put on and is placed on 5 DEG C of refrigerators and save backup；
(4) commercially available Corn bread is loaded in kraft paper bag to be placed in 120 DEG C of baking ovens and toasts 0.5h, put on label and be placed on 5 DEG C
Refrigerator saves backup；
(5) yeast powder is commercially available low sugar Angel Yeast, and being placed in 5 DEG C of refrigerators without sterilizing saves backup；
(6) ripe soy meal, grey vegetable powder, wheat bran, yeast powder, maize flour and casein are weighed respectively in the above feed ratio, then
It is put in homogenizer；
(7) it weighs agar powder to be placed in pot, the distilled water of measurement is poured into pot, slowly rock pot until agar dissolving, then will
Aqueous agar solution boils, and is during which stirred continuously, and is transferred in homogenizer after infusion 5min again after boiling；
(8) it by the adherent powder stirring and dissolving of homogenizer, waits for that all mixtures are stirring evenly and then adding into soybean edible oil, is again stirring for
Uniformly；
(9) make mixture fast cooling that the cholesterol weighed, ascorbic acid, right be added afterwards to 60 DEG C or less draught cupboard opening
Methyl hydroxybenzoate and sorbic acid pour into porcelain dish of the preprepared through high-temperature sterilization after stirring evenly, wait for its cooling
After solidification, it is cut into 2-3mm thin slices by knife and is placed in 5 DEG C of refrigerators and save backup.
3. Artificial Diets For Beet Armyworm high-volume described in claim 1, the method for continuously rearing beet armyworm, according to the following steps
It carries out：
(1) ovum-newly hatched larvae：By production have the ovum paper of Beet Armyworm Eggs with scissors be cut into the small scraps of paper be placed on commercially available 90mm ×
In 15mm aseptic plastic culture dishes, after ovum blackening, the claim that two block sizes are 20mm × 20mm × 2mm is gripped with tweezers
Artificial Diets For Beet Armyworm described in 1 is placed in culture dish, and pay attention to be sure not feed being pressed on pieces of an egg, set 6 layers of 100mm ×
150mm toilet papers abandon ovum paper on culture dish, covering culture ware lid after waiting for larva to hatch completely；
(2) 1-3 instar larvaes：1-3 instar larvaes are raised in culture dish always, larva 200-300 heads in each culture dish, each
Two pieces of 40mm × 20mm × 2mm Artificial Diets For Beet Armyworms described in claim 1 are placed in culture dish；
(3) 3 instar larvaes-pupate：It grows to 3 ages, is allocated larva into other culture dishes with writing brush, each after newly hatched larvae
Culture dish 10-17 heads place the beet armyworm people described in claim 1 of two pieces of 40mm × 20mm × 2mm in each culture dish
Work feed waits for long to 4 age ends removing excreta, addition one block length 30mm × 20mm × 2mm beet armyworms described in claim 1
Man-made feeds, and a toilet paper is replaced, it supports to just feed is eaten up when prepupal period, and be allowed to pupate in culture dish；
(4) it pupates-sprouts wings：With small spoon pupa dug out after pupating 3-4 days and is respectively placed in bottom after distinguishing male and female and is placed with filter paper
100mm × 25mm glass culture dish in, then cover upper 90mm × 21cm glass paper caps, then covering on top with nylon gauze uses
With ventilation, its emergence is then waited for；
(5) emergence-oviposition：Using household dustbin with holes put on polybag as mating cage, in bottom layer overlay filter paper or
Newspaper inside puts 10% honey as food；Using 190mm × 110mm 80g brown paper as ovum card, brown paper is before the use
10-15 foldings need to be folded, by the adult after emergence according to male and female 2:Totally 100 adults are put into mating cage 1 ratio, mate one day
After be put into ovum card, replace ovum card two days later, replace deutovum card within every two days later, and replace food daily；
Environmental condition in the insectary is that temperature is 27 ± 1 DEG C, indoor relative humidity 40%-50%, photoperiod L14
∶D10。
4. according to the method described in claim 3, wherein insectary need to irradiate 2h, and every 3 after often raising a generation under ultraviolet lamp
It is primary by insectary windowing ventilation.
5. according to the method described in claim 3,200-300 ovum are placed in the step (1) in each culture dish.
6. according to the method described in claim 3, at most putting 50 pupas in each culture dish in the step (4).
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| CN105145495B (en) * | 2015-09-10 | 2017-10-27 | 延安大学 | Jujube tree steals the artificial breeding method of poison moth |
| CN105192263A (en) * | 2015-10-09 | 2015-12-30 | 刘新霞 | Armadillidium breeding fodder and preparation method thereof |
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| CN106306389A (en) * | 2016-08-19 | 2017-01-11 | 华巧波 | Fodder for improving breeding efficiency of beet armyworm |
| CN106259221B (en) * | 2016-08-19 | 2019-08-27 | 华巧波 | A method of raising beet armyworm |
| CN106942157B (en) * | 2017-03-04 | 2019-10-11 | 云南农业大学 | A kind of Moringa Nao snout moth's larva method for breeding using man-made feeds |
| CN106954765B (en) * | 2017-04-11 | 2021-03-02 | 广东人为峰健康管理有限公司 | Insect feed and preparation method and application thereof |
| CN110089642A (en) * | 2019-06-15 | 2019-08-06 | 安徽省农业科学院植物保护与农产品质量安全研究所 | A kind of Spodopterafrugiperda man-made feeds |
| CN111328935B (en) * | 2020-04-08 | 2022-12-23 | 四川省农业科学院植物保护研究所 | Feed for beet armyworm and preparation method and application thereof |
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