CN110089642A - A kind of Spodopterafrugiperda man-made feeds - Google Patents
A kind of Spodopterafrugiperda man-made feeds Download PDFInfo
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- CN110089642A CN110089642A CN201910519647.8A CN201910519647A CN110089642A CN 110089642 A CN110089642 A CN 110089642A CN 201910519647 A CN201910519647 A CN 201910519647A CN 110089642 A CN110089642 A CN 110089642A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/111—Aromatic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/195—Antibiotics
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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Abstract
It is composed of the following components the invention discloses a kind of Spodopterafrugiperda man-made feeds: kidney bean powder, wheat germ powder, maize leaves powder, soy meal, casein, yeast powder, agar, ascorbic acid, methyl p-hydroxybenzoate, sorbic acid, tetracycline, multi-vitamins.Man-made feeds raw material of the invention are easy to get, and preparation is simple, and low in cost, feeding effect is good.
Description
Technical field
The present invention relates to technical field of insect feeding more particularly to a kind of Spodopterafrugiperda man-made feeds and its preparation sides
Method, and corresponding Spodopterafrugiperda rearing method.
Background technique
Spodopterafrugiperda Spodoptera frugiperda (Smith) is commonly called as autumn armyworm (Fall Armyworm), belongs to
Lepidoptera Noctuidae Spodoptera is a kind of polyphagous pest-insect for originating in american torrid zone and subtropical zone, at " 2017
Cosmopolitian plant status report " the big plant insect in the world ten is chosen as by International Center for Agriculture and Biological Sciences, have appropriate area it is wide,
The features such as ability of migrating strong (can fly every night 100 kilometers at moth), reproduction speed are fast, gluttony is caused harm again, prevention and control difficulty is big.2016
Discovery invasion in January in year Africa is only just intruded into two years most of African on the south the Sahara in December, 2018
Country and the India in Asia, Sri Lanka, Thailand, Yemen and Burma and Bangladesh etc. are national, on January 18th, 2019
It announces the worm and invades China from Burma in state's agricultural rural area portion.
Spodopterafrugiperda biology, adaptation Journal of Sex Research, efficient medicament and recipe determination, resistance risk assessment, natural enemy are numerous
It grows, the element task of the multinomial research such as biological control requires a large amount of normal development, breeding time consistent Spodopterafrugiperda
Larva.The country there is no Spodopterafrugiperda man-made feeds to report at present.
The present invention provides a kind of man-made feeds of suitable Spodopterafrugiperda raising, together for deficiency existing for above-mentioned field
When the preparation method of the man-made feeds is provided and raises Spodopterafrugiperda method, to realize the extensive, long of standard worm sources
Phase, continuously rearing.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of Spodopterafrugiperda man-made feeds.
The invention solves another technical problem be to provide the preparation methods of above-mentioned Spodopterafrugiperda man-made feeds
The invention solves be to provide a kind of Spodopterafrugiperda rearing method there are one technical problem.
The invention solves there are one technical problem be to provide it is a kind of for raising artificial porous of Spodopterafrugiperda
Rearing tray.
For Spodopterafrugiperda man-made feeds, the technical solution adopted by the present invention is that, a kind of Spodopterafrugiperda man-made feeds,
It is composed of the following components:
Kidney bean powder, wheat germ powder, maize leaves powder, soy meal, casein, yeast powder, agar, ascorbic acid, para hydroxybenzene first
Sour methyl esters, sorbic acid, tetracycline, multi-vitamins.
Preferably, above-mentioned Spodopterafrugiperda man-made feeds, composed of the following components:
Kidney bean powder 100g, wheat germ powder 80g, maize leaves powder 50g, soy meal 50g, casein 50g, yeast powder 60g, agar
35g, ascorbic acid 6g, methyl p-hydroxybenzoate 5g, sorbic acid 3g, tetracycline 0.25g, multi-vitamins 10g, aqua sterilisa
2000ml。
As further preferred, above-mentioned Spodopterafrugiperda man-made feeds, step in the following order is prepared:
(1) preparation of kidney bean powder: being ground into powdery for kidney bean, and ground kidney bean powder is then put into 120 DEG C of ovens and is dried
Roasting 2h, obtains kidney bean powder and is placed in 4 DEG C of environment to save, spare；
(2) preparation of wheat germ powder: taking wheat seed, is put into 50~55 DEG C of warm water of 5 times of wheat seed volume amounts, will be small
Wheat Seeds immerse warm water and are stirred continuously, and stir to water temperature drop to 30 DEG C of stoppings, impregnating 8~12h, inhale wheat seed sufficiently
Receive moisture.Then moisture is drained, and after being wrapped up with wet gauze, is placed in 25 DEG C of progress vernalization；It grows to malt to 0.3~0.8cm,
Wheat seeds are placed under sunlight and are ground into powdery after natural drying, are put into 120 DEG C of oven cooking cycle 1h, wheat germ powder is obtained and is placed in 4 DEG C
It is saved in environment, it is spare；
(3) preparation of maize leaves powder: fresh corn blade is cleaned, and is put in naturally dry under sunlight, smashes it through 90 mesh
Sieve takes the maize leaves powder under sieve to be put into enamel tray, in 120 DEG C of oven cooking cycle 0.5h, obtains maize leaves powder and be placed in 4 DEG C of environment
It saves, it is spare；
(4) preparation of soy meal: being ground into powdery for soybean, and ground soy meal is then put into 120 DEG C of ovens and is dried
Roasting 2h, obtains soy meal and is placed in 4 DEG C of environment to save, spare；
(5) yeast powder: the pigeon bought from market raises brewer's yeast used, is put into 120 DEG C of oven cooking cycle 1h, obtains ferment
Female powder and being placed in 4 DEG C of environment saves, spare；
(6) component formula for pressing above-mentioned man-made feeds, weighs kidney bean powder, wheat germ powder, maize leaves powder, soy meal, ferment respectively
Female powder and casein are put in clean blender, stir evenly, and after 850mL aqua sterilisa is added, are mixed after mixing evenly
It is spare to close object A；
(7) press above-mentioned man-made feeds component formula, weigh respectively ascorbic acid, methyl p-hydroxybenzoate, sorbic acid,
Tetracycline, vitamin are added aqua sterilisa 50mL, it is spare to obtain mixture B after mixing evenly after mixing evenly；
(8) component formula for pressing above-mentioned man-made feeds, weighs agar and is put in container, and 300ml aqua sterilisa is added, sufficiently soaks
Bubble；It takes 800mL aqua sterilisa in micro-wave oven simultaneously, is heated to 75~85 DEG C, and pour into above-mentioned agar soak, be sufficiently stirred
It is even, it is spare that agar solution is made；
(9) by agar solution, mixture A is poured into, 45~55 DEG C is down to its temperature, mixture B is added, after mixing evenly
It pours into the vessel of prior sterilization treatment, arrives Spodopterafrugiperda man-made feeds after its cooled and solidified, be put into 4 DEG C of environment
Middle preservation, it is spare.
For Spodopterafrugiperda man-made feeds high-volume, the method for continuously rearing Spodopterafrugiperda, the skill that the present invention uses
Art scheme is, comprising the following steps:
(1) preparation of porous rearing tray: the porous rearing tray includes multiple length × width × heights with same size
Raising hole, which is connected with each other and arranges, is combined into a whole rearing tray disk body；The raising hole is with the plastic film envelope with through-hole
Mouthful, the diameter of through-hole is 0.5~2.0mm above the plastic film of sealing, and the density of through-hole is 7~10/cm2；Wherein, individually
The porous rearing tray of low instar larvae that the length × width × height specification for raising hole is 5 × 5 × 3cm is poly- suitable for ovum-low instar larvae stage
Collection raising, the porous rearing tray of 3 instar larvaes that the single length × width × height specification for raising hole is 2 × 2 × 3cm are suitable for 3 instar larvaes-change
The single head in pupa stage is raised；
(2) Spodopterafrugiperda man-made feeds are put into each raising hole of the porous rearing tray of low instar larvae to 1/2 hole depth,
And punch after being handled with high-temperature sterilization, on superclean bench, making a call to a diameter in feed center position is 0.8cm circular hole；
(3) ovum-low instar larvae: the meche for having Spodopterafrugiperda ovum will be produced, bulk is cut into according to size of egg masses, is placed in band
In lid enamel tray, enamel pan bottom spreads the wet sponge that a layer thickness is 0.5cm first, applies 4 layers of dry filter paper again thereon；Keep humidity
While, avoid the direct contact wetting of pieces of an egg；After ovum gradually blackening, the porous raising of low instar larvae is put in tweezers clamping pieces of an egg
In the raising hole of disk, 1 piece of pieces of an egg is put in every raising hole, is sure not for feed to be pressed on pieces of an egg；By the porous rearing tray of low instar larvae
Raising hole is raised with being put into artificial climate receptacle after narrow meshed plastic film sealing, until larva grew to for 3 ages, raising
Period supplements fresh man-made feeds by feeding situation according to Spodopterafrugiperda man-made feeds；By the plastics of sealing when supplement feed
Film is opened, after adding feed, again with plastic film sealing；
(4) 3 instar larvaes-pupate: Spodopterafrugiperda is put into each raising hole of the porous rearing tray of 3 instar larvaes and is manually raised
3 instar larvae single head of Spodopterafrugiperda is accessed raising hole, by the raising hole of the porous rearing tray of 3 instar larvaes with band to 1/2 hole depth by material
Artificial climate receptacle is put into after the plastic film sealing of aperture to raise to pupating, according to feed by feeding situation during raising,
Add fresh man-made feeds；The plastic film of sealing is opened when supplement feed, after adding feed, again with narrow meshed plastics
Film seal；
(5) it pupates-sprouts wings: after pupating 3~4 days, opening the raising hole of plastic packaging and take out mature pupa, after distinguishing male and female,
Bottom is respectively placed in be covered with wet filter paper, top opening and cover the length × width × height specification of nylon grenadine as 15 × 8 × 5cm's
Insect box waits its emergence；
(6) emergence-oviposition: prepare the cage with mesh that length × width × height specification is 20 × 30 × 25cm as mating
Cage；Spodopterafrugiperda adult after emergence is put into mating cage according to the ratio of sex ration 2: 1 for laying eggs；It is hung in cage white
Dyed yarn cloth is used as oviposition medium, puts honey sugar and water mixture as food in cage；Mating is put into oviposition card after one day, later every
The primary oviposition card of its replacement, and supplement food；Honey sugar and water mixture is by honey: sugar: water by weight 0.5: 0.5: 9 prepare and
At.
Preferably, needing to irradiate 2h after the every raising generation of artificial climate receptacle in the UV lamp, and every 3 days will be artificial
The windowing ventilation of weather receptacle is primary.
Preferably, all non-disposable utensils needed for supporting worm, it is necessary to pass through sterilization treatment, wherein glass and enamelware
For tool with 121 DEG C of high-temperature sterilization 20min, plastic ware, which is equal under ultraviolet lamp, irradiates 2h.
Preferably, the environmental condition of artificial climate receptacle is 27 ± 1 DEG C, larva raising relative humidity 40~50%,
Imago breeding relative humidity 60~70%, photoperiod illumination: dark=14: 10h.
For porous rearing tray, the technical solution adopted by the present invention is that, including cubic shaped and opening up raising
Hole；The opening edges in multiple raising holes are combined into a whole porous rearing tray disk body along being connected with each other simultaneously to arrange, and when raising waits raising
It supports after being put into feed and worm's ovum or larva in hole, i.e., is raised after the opening for raising hole being applied the plastic film sealing with through-hole
It supports.
Preferably, the diameter of through-hole is 0.5~2.0mm above plastic film, the density of through-hole is 7~10/cm2；
The length × width × height specification in single raising hole is respectively 2 × 2 × 3cm or 5 × 5 × 3cm.
Preferably, porous rearing tray disk body is by one piece plastics plate, integrally compacting is formed.
The beneficial effects of the present invention are:
(1) raw material involved in man-made feeds formula of the invention are easy to obtain, and manufacture craft is simple, at low cost
It is honest and clean, long shelf-life.
(2) kidney bean powder used in man-made feeds of the invention, containing saponin(e, uremic enzyme and a variety of globulin etc. it is unique at
Point, have and improve immunity of organisms, the ability to enhance disease resistance can significantly improve Spodopterafrugiperda survival rate of larvae, pupate
Rate reduces abnormal pupa rate.
(3) the maize leaves powder that man-made feeds of the invention use can increase the fiber content of feed, reduce feed viscosity,
Feed oxygen content is improved, larva living environment is improved.
(4) method for melting agar in man-made feeds manufacturing process of the invention using micro-wave oven reheating, guarantees fine jade
While rouge viscosity, solves the problems, such as to be easy viscous pot during open firing agar, is charred and agar is caused to lose, while
It avoids in heating process, the moisture loss as caused by transpiration.
(5) it during man-made feeds breeding grub of the invention, has used disposably by multiple raising hole arrangement groups
At porous rearing tray, that is, tear open and use, it is cheap without sterilizing, abandon, saved needed for a large amount of cleanings, sterilizing after
Manually.
(6) method for breeding of the invention is during Adult worms producting eggs, uses white meche as oviposition medium, and by its
Mating cage is hung on, is easily changed, adult escapes during avoiding egg collection.
Detailed description of the invention
The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.
Fig. 1 is the main view (bottom) of the porous rearing tray embodiment of the present invention.
Fig. 2 is the top view of the porous rearing tray embodiment of the present invention.
Fig. 3 is partial view at the A of Fig. 2.
Fig. 4 is the rearview of the porous rearing tray embodiment of the present invention.
Marked in the figure: 1- disk body, 2- raises hole, 3- plastic film, 4- through-hole.
Specific embodiment
Embodiment 1
A kind of Spodopterafrugiperda man-made feeds, composed of the following components:
Kidney bean powder 100g, wheat germ powder 80g, maize leaves powder 50g, soy meal 50g, casein 50g, yeast powder 60g, agar
35g, ascorbic acid 6g, methyl p-hydroxybenzoate 5g, sorbic acid 3g, tetracycline 0.25g, multi-vitamins 10g, aqua sterilisa
2000ml。
Embodiment 2
Above-mentioned Spodopterafrugiperda man-made feeds, step in the following order are prepared:
(1) preparation of kidney bean powder: buying kidney bean from market, is ground into powdery using medicinal herb grinder, then will be ground
Kidney bean powder be put into enamel tray, be placed in 120 DEG C of oven cooking cycle 2h, obtain kidney bean powder and be placed in 4 DEG C of refrigerators saving, it is spare；
(2) preparation of wheat germ powder: buying wheat seed from market, is put into 50~55 DEG C of warm water of 5 times of seed volume amounts,
Seed is immersed into warm water and is stirred continuously, is stirred to water temperature drop to 30 DEG C of stoppings, 8~12h is impregnated, seed is made to fully absorb water
Point, then moisture is drained, and after being wrapped up with wet gauze, be placed in 25 DEG C of progress vernalization；To malt length to 0.5cm or so, by wheat
Kind is placed in naturally dry under sunlight, is ground into powdery using medicinal herb grinder, is put into enamel tray in 120 DEG C of oven cooking cycle 1h, obtains
To wheat germ powder and 4 DEG C of refrigerators preservations are placed in, it is spare；
(3) preparation of maize leaves powder: fresh corn blade is cleaned, and is put in naturally dry under sunlight, smashes it through 90 mesh
Sieve takes the lower maize leaves powder of sieve to be put into enamel tray, is placed in 120 DEG C of oven cooking cycle 0.5h, obtains maize leaves powder and is placed in 4 DEG C of refrigerators guarantors
It deposits, it is spare；
(4) preparation of soy meal: buying soybean from market, is ground into powdery using medicinal herb grinder, then will be ground
Soy meal be put into enamel tray, 120 DEG C of oven cooking cycle 2h obtain soy meal and are placed in 4 DEG C of refrigerators saving, spare；
(5) yeast powder: the pigeon bought from market raises brewer's yeast used, is put into enamel tray, 120 DEG C of oven cooking cycles
1h obtains yeast powder and is placed in 4 DEG C of refrigerators preservations, spare；
(6) by the component formula of the man-made feeds of embodiment 1, kidney bean powder, wheat germ powder, maize leaves powder, soybean are weighed respectively
Powder, yeast powder and casein are put in clean blender, stir evenly, after 850mL aqua sterilisa is added, after mixing evenly
It is spare to mixture A；
(7) by the component formula of the man-made feeds of embodiment 1, ascorbic acid, methyl p-hydroxybenzoate, mountain are weighed respectively
Pears acid, tetracycline, vitamin are added aqua sterilisa 50mL, it is spare to obtain mixture B after mixing evenly after mixing evenly；
(8) it by the component formula of the man-made feeds of embodiment 1, weighs agar and is put in container, 300ml aqua sterilisa is added,
Sufficiently impregnate；It takes 800mL aqua sterilisa in micro-wave oven simultaneously, is heated to 80 DEG C or so, and pour into above-mentioned agar soak, sufficiently stirs
It mixes uniformly, it is spare that agar solution is made；
(9) agar solution is poured into mixture A, is down to 50 DEG C or so to its temperature, adds mixture B, stir evenly
It is poured into the enamel tray of prior sterilization treatment afterwards, arrives Spodopterafrugiperda man-made feeds after its cooled and solidified, and be put into 4
DEG C refrigerator saves backup.
Embodiment 3
Using above-mentioned Spodopterafrugiperda man-made feeds high-volume, the method for continuously rearing Spodopterafrugiperda, including following step
It is rapid:
(1) preparation of porous rearing tray: porous rearing tray includes the raising of multiple length × width × heights with same size
Hole, which is connected with each other and arranges, is combined into a whole rearing tray disk body, and raises hole with the plastic film sealing with through-hole, sealing
Plastic film above the diameter of through-hole be 0.5~2.0mm, the density of through-hole is 7~10/cm2；Wherein, hole is individually raised
Length × width × height specification be 5 × 5 × 3cm the porous rearing tray of low instar larvae be suitable for the aggregation of ovum-low instar larvae stage raising,
The porous rearing tray of 3 instar larvaes that the length × width × height specification in single raising hole is 2 × 2 × 3cm, which is suitable for 3 instar larvaes-, pupates the stage
Single head raising；
(2) Spodopterafrugiperda man-made feeds are put into each raising hole of the porous rearing tray of low instar larvae to 1/2 hole depth,
And punch after being handled with high-temperature sterilization, on superclean bench, making a call to a diameter in feed center position is 0.8cm circular hole；
(2) ovum-low instar larvae: the meche for having Spodopterafrugiperda ovum will be produced, bulk is cut into according to size of egg masses, is placed in band
In lid enamel tray, enamel pan bottom spreads the wet sponge that a layer thickness is 0.5cm first, applies 4 layers of dry filter paper again thereon；Keep humidity
While, avoid the direct contact wetting of pieces of an egg；After ovum gradually blackening, the porous raising of low instar larvae is put in tweezers clamping pieces of an egg
In the raising hole of disk, 1 piece of pieces of an egg is put in every raising hole, is sure not for feed to be pressed on pieces of an egg；By the porous rearing tray of low instar larvae
Raising hole is raised with being put into artificial climate receptacle after plastic film sealing (i.e. plastic packaging), until larva grew to for 3 ages；Raising
Period supplements fresh man-made feeds by feeding situation according to Spodopterafrugiperda man-made feeds；By the plastics of sealing when supplement feed
Film is opened, after adding feed, again with plastic film sealing；
(3) 3 instar larvaes-pupate: 3 instar larvaes that the length × width × height specification for preparing single raising hole is 2 × 2 × 3cm are more
Hole rearing tray is put into Spodopterafrugiperda man-made feeds to 1/2 hole depth in each raising hole of the porous rearing tray of 3 instar larvaes, incites somebody to action
3 instar larvae single head of Spodopterafrugiperda access raising hole, (i.e. with plastic film sealing by the raising hole of the porous rearing tray of 3 instar larvaes
Plastic packaging) after be put into artificial climate receptacle and raise to pupating, fresh man-made feeds are during which added by feeding situation according to feed；
The plastic film of sealing is opened when supplement feed, after adding feed, again with plastic film sealing；
(4) it pupates-sprouts wings: after pupating 3~4 days, taking out mature pupa and be respectively placed in bottom after distinguishing male and female and be covered with
The insect box that the length × width × height specification of wet filter paper, top opening and covering nylon grenadine is 15 × 8 × 5cm, waits its emergence；
(5) emergence-oviposition: prepare the cage with mesh that length × width × height specification is 20 × 30 × 25cm as mating
Cage；Spodopterafrugiperda adult after emergence is amounted to 90 adults according to the ratio of sex ration 2: 1 to be put into mating cage for producing
Ovum；White meche is hung in cage and is used as oviposition medium, and put in cage by honey: sugar: water is formulated by weight 0.5: 0.5: 9
Honey sugar and water mixture is as food；Mating is put into oviposition card after one day, the primary oviposition card of replacement daily later, and supplements food
Object.
Need to pay attention to following problems:
1) need to irradiate 2h in the UV lamp after the every raising generation of artificial climate receptacle, and every 3 days raise artificial climate
It is primary to support room windowing ventilation.
2) all non-disposable utensils needed for supporting worm, it is necessary to pass through sterilization treatment, wherein 121 DEG C of high-temperature sterilizations of glass wares
20min, plastic ware, which is equal under ultraviolet lamp, irradiates 2h.
3) environmental condition of artificial climate receptacle is 27 ± 1 DEG C, larva raising relative humidity 40~50%, imago breeding
Relative humidity 60~70%, photoperiod illumination: dark=14: 10h.
Embodiment 4
It is a kind of as disposable porous rearing tray, by 25 cubic shapeds and 2 groups opening up of raising hole
At (Fig. 1 is seen in bottom), wherein the opening edge in 25 raising holes along interconnection and arranges the porous raising for being combined into one piece of entirety
Disk disk body 1 (Fig. 2) when raising, is stayed in raising hole and is put into after feed and worm's ovum or larva i.e. to raising the opening in hole with band
There is the plastic film sealing of through-hole 4, i.e., is raised again after raising hole 2 being applied the plastic film 3 (Fig. 3) with through-hole, in addition
Entire rearing tray disc surfaces after plastic packaging are as shown in Figure 4.
The diameter of through-hole is 1.0mm above the plastic film of sealing, and the density of through-hole is 9/cm2。
Hole will be raised with narrow meshed plastic film sealing, can guarantee the relatively closed of larva raising space, prevent children
Worm escape.Aperture on plastic film also basic guarantee raising hole inner air flow simultaneously, grows for larva and provides aerobic ring
Border, and the raising hole after sealing can reduce pathogenic bacterial infection as best one can, and avoid different breeding room cross-infections, improve
Survival rate of larvae.
Using one piece plastics plate, integrally compacting is formed the porous rearing tray disk body 1 of the present embodiment, is killed before factory
Bacterium disinfection and aseptic packaging, when use, which tears open, to be used, and without sterilizing, is directly abandoned after use.
Each raising hole size specification is depending on the larva worm age grade section raised.In the present embodiment, hole is individually raised
Length × width × height specification be respectively 2 × 2 × 3cm or two kinds of 5 × 5 × 3cm, wherein individually raising hole specification is 2 × 2 × 3cm
The ovum suitable for Spodopterafrugiperda-low instar larvae stage aggregation raising, single hole specification of raising is that 5 × 5 × 3cm is suitable for grass
3 instar larvaes-of ground Beet armyworm pupate the stage single head raising.
Application method:
Insect feedstuff first is added in each raising hole in monolith disposably porous rearing tray disk body, and in feed
The circular hole that a diameter is 0.8cm is made a call to puncher, insect worm's ovum or larva is put into the circular hole of insect raising, reuses plastics
Each raising hole is added plastic film sealing by film capping machine, and finally upper tens pin holes are pricked in face on a plastic film, is sent into people
Work weather culturing room carries out insect culture, directly abandons after the completion of insect culture.
The disposable porous rearing tray that multiple raising holes of the present embodiment rearrange uses the children in Spodopterafrugiperda
Worm breeding process, that is, tear open and use, cheap without sterilizing, abandons after, saves artificial needed for a large amount of cleanings, sterilizing.
Embodiment 5
Compliance test result
Selected insect source: worm sources come from Anhui Province Huangshan corn field, raise through indoor corn tissue to the F1 generation of oviposition
Newly hatched larvae.
For trying feed: being produced by the man-made feeds formula of embodiment 1 and the feed producing method of embodiment 2.
Test method: the method for breeding of embodiment 3 is pressed in test worm raising.3 repetitions of every processing, every repetition is for trying larva 30
Head, artificial climate receptacle environmental condition: 27 ± 1 DEG C, larva raising relative humidity 40~50%, imago breeding relative humidity 60
~70%, the illumination of photoperiod: dark=14: 10h.In breeding process record larvae alive number, emergence number, oviposition figureofmerit,
Larva goes through phase, pupa time, preoviposition period, calculates survival rate of larvae, eclosion rate, single female average accumulated egg laying amount.
Test data is analyzed using SAS software, and data are indicated with mean+SD in text.
Test result:
With the effect such as table 1 of the man-made feeds raising Spodopterafrugiperda of the present embodiment:
Table 1
| Survival rate of larvae (%) | 83.3±0.027 |
| Eclosion rate (%) | 93.4±1.73 |
| Larva goes through the phase (d) | 17.3±1.20 |
| Pupa time (d) | 10.4±1.41 |
| Preoviposition period (d) | 1.5±1.49 |
| Single female eggs on average amount (grain) | 480.8±166.16 |
As seen from Table 1, the Spodopterafrugiperda raised with the man-made feeds of the present embodiment, survival rate of larvae 83.3%, plumage
Rate is 93.4%, single female eggs on average amount 480.8.
The embodiments of the present invention described above are not intended to limit the scope of the present invention.It is any in the present invention
Spirit and principle within made modifications, equivalent substitutions and improvements etc., should be included in claim protection model of the invention
Within enclosing.
Claims (10)
1. a kind of Spodopterafrugiperda man-made feeds, which is characterized in that composed of the following components:
Kidney bean powder, wheat germ powder, maize leaves powder, soy meal, casein, yeast powder, agar, ascorbic acid, P-hydroxybenzoic acid first
Ester, sorbic acid, tetracycline, multi-vitamins.
2. Spodopterafrugiperda man-made feeds as described in claim 1, which is characterized in that composed of the following components:
Kidney bean powder 100g, wheat germ powder 80g, maize leaves powder 50g, soy meal 50g, casein 50g, yeast powder 60g, agar 35g resist
Bad hematic acid 6g, methyl p-hydroxybenzoate 5g, sorbic acid 3g, tetracycline 0.25g, multi-vitamins 10g, aqua sterilisa 2000ml.
3. Spodopterafrugiperda man-made feeds as claimed in claim 2, which is characterized in that step in the following order is prepared into
It arrives:
(1) preparation of kidney bean powder: being ground into powdery for kidney bean, and ground kidney bean powder is then put into 120 DEG C of oven cooking cycle 2h,
It obtains kidney bean powder and is placed in 4 DEG C of environment to save, it is spare；
(2) preparation of wheat germ powder: taking wheat seed, is put into 50~55 DEG C of warm water of 5 times of wheat seed volume amounts, by Wheat Species
Son immerses warm water and is stirred continuously, and stirs to water temperature drop to 30 DEG C of stoppings, impregnating 8~12h, wheat seed is made to fully absorb water
Point.Then moisture is drained, and after being wrapped up with wet gauze, is placed in 25 DEG C of progress vernalization；It is long to 0.3~0.8cm to malt, by wheat
Kind is placed under sunlight is ground into powdery after natural drying, is put into 120 DEG C of oven cooking cycle 1h, obtains wheat germ powder and be placed in 4 DEG C of environment
Middle preservation, it is spare；
(3) preparation of maize leaves powder: fresh corn blade is cleaned, and is put in naturally dry under sunlight, is smashed it through 90 meshes, is taken
Maize leaves powder under sieve is put into enamel tray, in 120 DEG C of oven cooking cycle 0.5h, obtains maize leaves powder and be placed in 4 DEG C of environment to save,
It is spare；
(4) preparation of soy meal: being ground into powdery for soybean, and ground soy meal is then put into 120 DEG C of oven cooking cycle 2h,
It obtains soy meal and is placed in 4 DEG C of environment to save, it is spare；
(5) yeast powder: the pigeon bought from market raises brewer's yeast used, is put into 120 DEG C of oven cooking cycle 1h, obtains yeast powder
And be placed in 4 DEG C of environment and save, it is spare；
(6) by the component formula of man-made feeds described in claim 2, kidney bean powder, wheat germ powder, maize leaves powder, soybean are weighed respectively
Powder, yeast powder and casein are put in clean blender, stir evenly, after 850mL aqua sterilisa is added, after mixing evenly
It is spare to mixture A；
(7) by the component formula of man-made feeds described in claim 2, ascorbic acid, methyl p-hydroxybenzoate, mountain are weighed respectively
Pears acid, tetracycline, vitamin are added aqua sterilisa 50mL, it is spare to obtain mixture B after mixing evenly after mixing evenly；
(8) it by the component formula of man-made feeds described in claim 2, weighs agar and is put in container, 300m1 aqua sterilisa is added,
Sufficiently impregnate；It takes 800mL aqua sterilisa in micro-wave oven simultaneously, is heated to 75~85 DEG C, and pour into above-mentioned agar soak, sufficiently stirs
It mixes uniformly, it is spare that agar solution is made；
(9) by the agar solution, mixture A is poured into, 45~55 DEG C is down to its temperature, mixture B is added, after mixing evenly
It pours into the vessel of prior sterilization treatment, arrives Spodopterafrugiperda man-made feeds after its cooled and solidified, be put into 4 DEG C of environment
Middle preservation, it is spare.
4. Spodopterafrugiperda man-made feeds high-volume as described in claim 1, the method for continuously rearing Spodopterafrugiperda, including with
Lower step:
(1) preparation of porous rearing tray: the porous rearing tray includes the raising of multiple length × width × heights with same size
Hole, which is connected with each other and arranges, is combined into a whole porous rearing tray disk body；The raising hole is with the plastic film envelope with through-hole
Mouthful, the diameter of through-hole is 0.5~2.0mm above the plastic film of sealing, and the density of through-hole is 7~10/cm2；Wherein, individually
The porous rearing tray of low instar larvae that the length × width × height specification for raising hole is 5 × 5 × 3cm is poly- suitable for ovum-low instar larvae stage
Collection raising, the porous rearing tray of 3 instar larvaes that the single length × width × height specification for raising hole is 2 × 2 × 3cm are suitable for 3 instar larvaes-change
The single head in pupa stage is raised；
(2) Spodopterafrugiperda man-made feeds are put into 1/2 hole depth in each raising hole of the porous rearing tray of low instar larvae, be used in combination
Punch after high-temperature sterilization processing, on superclean bench, making a call to a diameter in feed center position is 0.8cm circular hole；
(3) ovum-low instar larvae: the meche for having Spodopterafrugiperda ovum will be produced, bulk is cut into according to size of egg masses, is placed in cover ward off
In porcelain dish, enamel pan bottom spreads the wet sponge that a layer thickness is 0.5cm first, applies 4 layers of dry filter paper again thereon；Keep the same of humidity
When, avoid the direct contact wetting of pieces of an egg；After ovum gradually blackening, the porous rearing tray of low instar larvae is put in tweezers clamping pieces of an egg
It raises in hole, 1 piece of pieces of an egg is put in every raising hole, is sure not for feed to be pressed on pieces of an egg；By the raising of the porous rearing tray of low instar larvae
Hole is raised with being put into artificial climate receptacle after narrow meshed plastic film sealing, until larva grew to for 3 ages, during raising
According to Spodopterafrugiperda man-made feeds by feeding situation, fresh man-made feeds are supplemented；By the plastic film of sealing when supplement feed
It opens, after adding feed, again with narrow meshed plastic film sealing；
(4) 3 instar larvaes-pupate: Spodopterafrugiperda man-made feeds are put into extremely in each raising hole of the porous rearing tray of 3 instar larvaes
3 instar larvae single head of Spodopterafrugiperda is accessed raising hole, by the raising hole of the porous rearing tray of 3 instar larvaes with band aperture by 1/2 hole depth
Plastic film sealing after be put into artificial climate receptacle and raise to pupating, according to feed by feeding situation, addition during raising
Fresh man-made feeds；The plastic film of sealing is opened when supplement feed, after adding feed, again with narrow meshed plastic film
Sealing；
(5) it pupates-sprouts wings: after pupating 3~4 days, opening the raising hole of plastic packaging and take out mature pupa, after distinguishing male and female, respectively
It is placed in bottom and is covered with the feeding worm that the length × width × height specification of wet filter paper, top opening and covering nylon grenadine is 15 × 8 × 5cm
Box waits its emergence；
(6) emergence-oviposition: prepare the cage with mesh that length × width × height specification is 20 × 30 × 25cm as the cage that mates；It will
Spodopterafrugiperda adult after emergence is put into mating cage according to the ratio of sex ration 2: 1 for laying eggs；White yarn is hung in cage
Cloth is used as oviposition medium, puts honey sugar and water mixture as food in cage；Mating is put into oviposition card after one day, daily more later
Primary oviposition card is changed, and supplements food；The honey sugar and water mixture is by honey: sugar: water by weight 0.5: 0.5: 9 prepare and
At.
5. Spodopterafrugiperda rearing method as claimed in claim 4, it is characterised in that: the artificial climate receptacle is every
Need to irradiate 2h after a raising generation in the UV lamp, and every 3 days primary by the windowing ventilation of artificial climate receptacle.
6. Spodopterafrugiperda rearing method as claimed in claim 4, it is characterised in that: all non-onces needed for supporting worm
Sex apparatus, it is necessary to pass through sterilization treatment, wherein with 121 DEG C of high-temperature sterilization 20min, plastic ware is equal to purple for glass and enamel utensil
2h is irradiated under outer lamp.
7. Spodopterafrugiperda rearing method as claimed in claim 4, it is characterised in that: the artificial climate receptacle
Environmental condition is 27 ± 1 DEG C, larva raising relative humidity 40~50%, imago breeding relative humidity 60~70%, photoperiod light
According to: dark=14: 10h.
8. a kind of porous rearing tray, which is characterized in that including cubic shaped and opening up raising hole；Multiple raisings
The opening edge in hole is combined into a whole porous rearing tray disk body along being connected with each other simultaneously to arrange, and is put into feeding in when raising hole to be raised
After material and worm's ovum or larva, i.e., raised after the opening for raising hole being applied narrow meshed plastic film sealing.
9. porous rearing tray as claimed in claim 8, which is characterized in that the diameter of aperture is 0.5 above the plastic film
~2.0mm, the density of aperture are 7~10/cm2；The length × width × height specification in the single raising hole is respectively 2 × 2 × 3cm
Or 5 × 5 × 3cm.
10. porous rearing tray as claimed in claim 8, which is characterized in that the porous rearing tray disk body is by one piece plastics plate
Integrally compacting is formed material.
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| CN110353133A (en) * | 2019-08-12 | 2019-10-22 | 云南省农业科学院农业环境资源研究所 | A kind of man-made feeds of Spodopterafrugiperda larva and its application |
| CN110367407A (en) * | 2019-09-02 | 2019-10-25 | 浙江省农业科学院 | A kind of Spodopterafrugiperda larva artificial feed and its preparation method and application |
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