CN110915761A - Indoor artificial propagation method for Spodoptera frugiperda in full-dark condition in young stage - Google Patents
Indoor artificial propagation method for Spodoptera frugiperda in full-dark condition in young stage Download PDFInfo
- Publication number
- CN110915761A CN110915761A CN201911231473.1A CN201911231473A CN110915761A CN 110915761 A CN110915761 A CN 110915761A CN 201911231473 A CN201911231473 A CN 201911231473A CN 110915761 A CN110915761 A CN 110915761A
- Authority
- CN
- China
- Prior art keywords
- spodoptera frugiperda
- artificial
- pupae
- larvae
- propagation method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000256251 Spodoptera frugiperda Species 0.000 title claims abstract description 73
- 238000000034 method Methods 0.000 title claims abstract description 29
- 235000013601 eggs Nutrition 0.000 claims abstract description 26
- 241000238631 Hexapoda Species 0.000 claims abstract description 15
- 238000009395 breeding Methods 0.000 claims abstract description 12
- 230000001488 breeding effect Effects 0.000 claims abstract description 12
- 230000032669 eclosion Effects 0.000 claims abstract description 10
- 238000011534 incubation Methods 0.000 claims abstract description 10
- 230000012447 hatching Effects 0.000 claims abstract description 7
- 230000003203 everyday effect Effects 0.000 claims abstract description 4
- 239000011521 glass Substances 0.000 claims abstract description 4
- 238000010008 shearing Methods 0.000 claims abstract description 3
- 239000000463 material Substances 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 239000003795 chemical substances by application Substances 0.000 claims description 12
- 239000000843 powder Substances 0.000 claims description 12
- 235000015097 nutrients Nutrition 0.000 claims description 11
- 238000007493 shaping process Methods 0.000 claims description 10
- 238000005303 weighing Methods 0.000 claims description 9
- 229920001817 Agar Polymers 0.000 claims description 8
- 239000008272 agar Substances 0.000 claims description 8
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 6
- 240000008042 Zea mays Species 0.000 claims description 6
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 6
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims description 6
- 235000005822 corn Nutrition 0.000 claims description 6
- 239000001763 2-hydroxyethyl(trimethyl)azanium Substances 0.000 claims description 3
- 235000019743 Choline chloride Nutrition 0.000 claims description 3
- 244000068988 Glycine max Species 0.000 claims description 3
- 235000010469 Glycine max Nutrition 0.000 claims description 3
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 claims description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- 241000209140 Triticum Species 0.000 claims description 3
- 235000021307 Triticum Nutrition 0.000 claims description 3
- 235000010323 ascorbic acid Nutrition 0.000 claims description 3
- 229960005070 ascorbic acid Drugs 0.000 claims description 3
- 239000011668 ascorbic acid Substances 0.000 claims description 3
- FCPVYOBCFFNJFS-LQDWTQKMSA-M benzylpenicillin sodium Chemical compound [Na+].N([C@H]1[C@H]2SC([C@@H](N2C1=O)C([O-])=O)(C)C)C(=O)CC1=CC=CC=C1 FCPVYOBCFFNJFS-LQDWTQKMSA-M 0.000 claims description 3
- 238000009835 boiling Methods 0.000 claims description 3
- 239000005018 casein Substances 0.000 claims description 3
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 claims description 3
- 235000021240 caseins Nutrition 0.000 claims description 3
- 235000012000 cholesterol Nutrition 0.000 claims description 3
- 229960003178 choline chloride Drugs 0.000 claims description 3
- SGMZJAMFUVOLNK-UHFFFAOYSA-M choline chloride Chemical compound [Cl-].C[N+](C)(C)CCO SGMZJAMFUVOLNK-UHFFFAOYSA-M 0.000 claims description 3
- BEFDCLMNVWHSGT-UHFFFAOYSA-N ethenylcyclopentane Chemical compound C=CC1CCCC1 BEFDCLMNVWHSGT-UHFFFAOYSA-N 0.000 claims description 3
- 235000013312 flour Nutrition 0.000 claims description 3
- 235000012907 honey Nutrition 0.000 claims description 3
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 claims description 3
- 229960000367 inositol Drugs 0.000 claims description 3
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 claims description 3
- 235000010199 sorbic acid Nutrition 0.000 claims description 3
- 229940075582 sorbic acid Drugs 0.000 claims description 3
- 239000004334 sorbic acid Substances 0.000 claims description 3
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 239000010935 stainless steel Substances 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 3
- 230000009469 supplementation Effects 0.000 claims description 3
- 230000009286 beneficial effect Effects 0.000 claims description 2
- 150000001875 compounds Chemical class 0.000 claims description 2
- 238000010411 cooking Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 235000019629 palatability Nutrition 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims 2
- 239000008213 purified water Substances 0.000 claims 2
- 241000256248 Spodoptera Species 0.000 claims 1
- 230000003247 decreasing effect Effects 0.000 claims 1
- 241000382353 Pupa Species 0.000 abstract 1
- 238000004166 bioassay Methods 0.000 abstract 1
- 241000607479 Yersinia pestis Species 0.000 description 3
- 235000021186 dishes Nutrition 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000255777 Lepidoptera Species 0.000 description 1
- 241000256259 Noctuidae Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 240000006394 Sorghum bicolor Species 0.000 description 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 1
- 231100000605 Toxicity Class Toxicity 0.000 description 1
- 230000003542 behavioural effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000005058 diapause Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 238000010025 steaming Methods 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Physiology (AREA)
- Birds (AREA)
- Insects & Arthropods (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention discloses an indoor artificial propagation method for Spodoptera frugiperda in the full-dark condition in the seedling stage. Adult Spodoptera frugiperda is raised in a transparent insect breeding tank according to the female-male ratio of about 1:1, and a little gauze is placed at the bottom of the tank to collect Spodoptera frugiperda eggs. Shearing off newly collected spodoptera frugiperda egg blocks on gauze every day, and placing the cut spodoptera frugiperda egg blocks into an incubation basin for incubation. After the spodoptera frugiperda larvae grow to three instars in the hatching pot, glass culture dishes are adopted for subpackaging, and the three instars and a small amount of artificial feed are placed in each culture dish and are continuously fed until the spodoptera frugiperda larvae pupate. Collecting pupa in the culture dish at later stage, and placing into a plastic box until eclosion. The raising conditions of spodoptera frugiperda in the egg stage, the larva stage and the pupal stage are that the temperature is 25 +/-0.5 ℃, the relative humidity is 65 +/-5%, and the photoperiod L and D are 0h and 24 h. The method for breeding Spodoptera frugiperda is simple and can meet the requirements of indoor bioassay tests on various insect states of Spodoptera frugiperda.
Description
Technical Field
The invention relates to a propagation method for crop pests, in particular to a propagation method for invasive pests, namely spodoptera frugiperda.
Background
Spodoptera frugiperda is native to tropical and subtropical regions of America, is a omnivorous pest of Noctuidae of Lepidotera of Lepidoptera, is harmful to more than 80 crops such as corn, rice, potato and sorghum, and can cause crop failure in harvest when serious. The method is characterized in that 1 month in 2019, the method invades Yunnan province of China, and then gradually spreads the crops which are harmful to 18 provinces (regions and cities) of the whole country.
In order to control the harm of the spodoptera frugiperda to crops in China and reduce economic loss, comprehensive treatment of the spodoptera frugiperda is urgently needed. A large number of indoor biological and behavioral observations and pesticide toxicity tests are of great significance for making perfect treatment measures, so that a large number of regularly developed test insects are obtained indoors, and the method is very important for developing research work on spodoptera frugiperda.
Domestic artificial feeds for spodoptera frugiperda have been reported, but improvements are needed in the aspect of specific artificial feeding methods.
Disclosure of Invention
The invention aims to provide a systematic indoor artificial propagation technology for spodoptera frugiperda in the full-dark condition in the seedling stage, which is simple and labor-saving in technical method and can quickly and effectively obtain spodoptera frugiperda in each insect state.
The technical scheme provided by the invention is as follows:
an indoor artificial propagation method for Spodoptera frugiperda in full-dark conditions in the seedling stage comprises the following steps:
A1. the artificial feed is prepared by weighing ① 400-500G of soybean flour, 50-150G of wheat germ powder, 100-250G of corn leaf powder and 1000-2000 ml of pure water, fully and uniformly mixing to form a main material of the spodoptera frugiperda artificial feed, cooking the main material for 30-60 min at 100-150 ℃ by using an autoclave, weighing 40-100G of agar powder and 1000-1500 ml of pure water by using ②, pouring the agar into a stainless steel container, boiling the water by using an electromagnetic oven to fully melt the agar to prepare a setting agent of the main material, weighing 50-100G of yeast by using ③, 20-60G of casein, 10-30G of p-hydroxyformate, 0.1-1G of cholesterol, 0.3-1.5G of inositol, 2-10G of sorbic acid, 1-5G of choline chloride, 5-20G of ascorbic acid, 2-8 compound tablets of Vb, 0.1-1G of penicillin G sodium salt, finally dissolving by using 300-1000 ml of pure water to prepare a nutrient solution, and adding the main material and the nutrient solution uniformly to form a finished product of spodoptera frugiperda artificial feed when the temperature of the main material and the setting agent is reduced to 40-60 ℃;
A2. in the adult stage: feeding male and female spodoptera frugiperda adults in a transparent insect breeding tank according to the proportion of 1:1, adding 10-15% of honey water every day, and placing a little gauze at the bottom of the tank; the total amount of male and female spodoptera frugiperda adults is 30-50;
A3. collecting egg masses: replacing the gauze at the bottom of the pot every 2 days, and shearing the spodoptera frugiperda egg blocks covered by the fuzz on the gauze taken out of the pot by using scissors and part of the gauze; putting the cut egg blocks into an incubation basin, putting two artificial feeds into the basin, wherein the positions where the egg blocks are placed are the upper surfaces of the artificial feeds; covering the incubation basin with a plastic disc to prevent larvae from escaping after eggs are incubated; incubating Spodoptera frugiperda eggs in an incubator under the conditions of 25 +/-0.5 ℃ of temperature, 65 +/-5% of relative humidity and light cycle L, D, of 0h and 24 h;
A4. larva breeding: keeping the Spodoptera frugiperda larvae to be raised in a hatching pot from egg hatching to before the Spodoptera frugiperda larvae are three years old, respectively putting the Spodoptera frugiperda larvae into glass culture dishes with the calibers of 15-20 cm for raising after the Spodoptera frugiperda larvae are three years old, putting 1-20 larvae of the Spodoptera frugiperda and three artificial feeds into each culture dish, and continuously raising until the larvae pupate; feeding Spodoptera frugiperda larvae in a culture box under the conditions of 25 +/-0.5 ℃ of temperature, 65 +/-5% of relative humidity and light cycle L, D being 0h and 24 h;
A5. collecting pupae: when the Spodoptera frugiperda pupae becomes hard and dark brown, taking the pupae out of the culture dish and putting the pupae into a plastic box until the pupae eclosion occurs; the emergence of the Spodoptera frugiperda pupae is carried out under the condition of full darkness;
A6. adult supplementation: regularly observing whether the plastic box for containing the pupae has imago eclosion or not, and connecting the newly eclosion imago into the insect feeding tank to form circulation.
According to the artificial propagation method, the artificial feed is prepared into a single block with the size of about 5cm in length, 3.5cm in width and 1.5cm in height.
The artificial propagation method can increase and decrease the content of each component in the basic formula of the artificial feed according to the proportion of the production amount of the feed.
According to the artificial propagation method, the corn leaf powder is added into the artificial feed, so that the palatability of the feed is improved.
In the process of preparing the artificial feed, the main material, the shaping agent and the nutrient solution are uniformly mixed by the artificial propagation method, the temperature is controlled to be 45-50 ℃, substances in the nutrient solution are easy to deform when the temperature is too high, and the substances are easy to solidify when the temperature is too low, so that the main material, the shaping agent and the nutrient solution are not beneficial to being fully and uniformly mixed.
The artificial propagation method is characterized in that in order to enable the spodoptera frugiperda imagoes to have enough activity space, the diameter of the insect breeding tank is 35-50 cm, and the height of the insect breeding tank is 20-40 cm.
The artificial propagation method is carried out after pupae are fully dissolved and become hard when the pupae of the Spodoptera frugiperda are collected so as to avoid damaging the pupae.
The indoor artificial propagation technology for Spodoptera frugiperda in the full-dark condition in the seedling stage has the following advantages:
1. the Spodoptera frugiperda is fed with artificial feed in the whole process, so that the dependence of feeding Spodoptera frugiperda on fresh and living plants is greatly reduced, and the optimization on time investment and space utilization is realized.
2. The Spodoptera frugiperda is fed in a standardized manner by adopting the artificial feed, so that standard unified insect test can be provided for indoor determination of biological characteristics such as ecological adaptability, drug resistance and the like of the Spodoptera frugiperda, and the accuracy and the feasibility of an experimental result are improved.
3. The characteristics that Spodoptera frugiperda does not have diapause are fully combined and utilized, the larva of Spodoptera frugiperda is raised under the full-dark condition, so that the time that the Spodoptera frugiperda larva crawls around is reduced, and the risk of killing the Spodoptera frugiperda larva among individuals during group raising is reduced.
Detailed Description
The present invention will be described in detail with reference to specific examples.
1. The artificial feed is prepared by ① weighing 450G of soybean flour, 100G of wheat germ powder, 150G of corn leaf powder and 1500ml of pure water, fully and uniformly mixing to form a main material of the spodoptera frugiperda artificial feed, steaming the main material for 40min at 120 ℃ by using an autoclave, weighing 60G of agar powder and 1200ml of pure water by ②, pouring the agar powder and the 1200ml of pure water into a stainless steel container, boiling the water by using an electromagnetic oven to fully melt the agar to prepare a shaping agent of the main material, ③ weighing 80G of yeast, 40G of casein, 15G of p-hydroxy formate, 0.6G of cholesterol, 0.7G of inositol, 6G of sorbic acid, 3G of choline chloride, 14G of ascorbic acid, composite Vb 5 tablets and 0.3G of penicillin G sodium salt, finally dissolving the raw material by using 500ml of pure water to prepare a nutrient solution, ④ adding the main material, the shaping agent and the nutrient solution together and fully and uniformly mixing when the temperature of the main material and the shaping agent is reduced to 50 ℃, and cooling to form.
2. In the adult stage: 30 male and female spodoptera frugiperda adults are respectively raised in a transparent insect breeding tank, 10% of honey water is added every day so that spodoptera frugiperda can smoothly reach the sexual maturity stage, a little gauze is placed at the bottom of the tank so that spodoptera frugiperda can lay egg blocks on the gauze, and the later-stage egg collection is facilitated.
3. Collecting egg masses: the gauze at the bottom of the pot is replaced every 2 days, the gauze taken out of the pot is carefully checked, and spodoptera frugiperda egg masses covered by the fuzz on the gauze are cut off together with part of the gauze by scissors. Putting the cut egg blocks into an incubation basin, putting two artificial feed blocks (the size of a single artificial feed block is about 5cm, the width is 3.5cm and the height is 1.5cm) into the basin, and putting the incubation block on the upper surface of the artificial feed. The incubation basin is covered with a plastic disc to prevent larvae from escaping after eggs are incubated. Incubation of spodoptera frugiperda eggs is carried out in an incubator under the conditions that the temperature is 25 +/-0.5 ℃, the relative humidity is 65 +/-5%, and the photoperiod L: D is 0h:24 h.
4. Larva breeding: the method comprises the steps of keeping Spodoptera frugiperda larvae to be raised in a hatching pot from egg hatching to three-year-old before Spodoptera frugiperda larvae, feeding the Spodoptera frugiperda larvae into glass culture dishes with the diameter of 18cm after the three-year-old larvae are raised, and continuously raising until the larvae pupate, wherein 10 heads and three artificial feeds (a single plate is about 5cm in length, 3.5cm in width and 1.5cm in height) are placed into each culture dish. The Spodoptera frugiperda larvae are raised in a culture box under the conditions that the temperature is 25 +/-0.5 ℃, the relative humidity is 65 +/-5%, and the photoperiod L: D is 0h:24 h.
5. Collecting pupae: when the Spodoptera frugiperda pupae becomes hard and dark brown, taking the pupae out of the culture dish and putting the pupae into a plastic box until the pupae eclosion occurs; the feeding conditions in the pupal stage are that the temperature is 25 +/-0.5 ℃, the relative humidity is 65 +/-5%, and the photoperiod L: D is 0h:24 h.
6. Adult supplementation: regularly observing whether the plastic box for containing the pupae has imago eclosion or not, and connecting the newly eclosion imago into the insect feeding tank to form virtuous cycle.
It will be understood that modifications and variations can be made by persons skilled in the art in light of the above teachings and all such modifications and variations are intended to be included within the scope of the invention as defined in the appended claims.
Claims (7)
1. An indoor artificial propagation method for Spodoptera frugiperda in full-dark conditions at the young stage is characterized by comprising the following steps:
A1. the artificial feed is prepared by ① weighing 400-500G of soybean flour, 50-150G of wheat germ powder, 100-250G of corn leaf powder and 1000-2000 ml of pure water, fully mixing the materials to form a main material of the spodoptera frugiperda artificial feed, cooking the main material for 30-60 min at 100-150 ℃ by using an autoclave, ② weighing 40-100G of agar powder and 1000-1500 ml of purified water, pouring the obtained mixture into a stainless steel container, boiling the water by using an electromagnetic oven to fully melt the agar, and preparing a shaping agent of the main material, ③ weighing 50-100G of yeast, 20-60G of casein, 10-30G of p-hydroxyformate, 0.1-1G of cholesterol, 0.3-1.5G of inositol, 2-10G of sorbic acid, 1-5G of choline chloride, 5-20G of ascorbic acid, 2-8 compound tablets, 0.1-1G of penicillin G sodium salt, finally dissolving the obtained mixture by using 300-1000 ml of purified water to prepare a nutrient solution, ④, and when the temperature of the main material and the shaping agent are reduced to 40-60 ℃, the main material is fully mixed with the spodoptera frugiura artificial feed, and then the finished product is cooled;
A2. in the adult stage: feeding male and female spodoptera frugiperda adults in a transparent insect breeding tank according to the proportion of 1:1, adding 10-15% of honey water every day, and placing a little gauze at the bottom of the tank; the total amount of male and female spodoptera frugiperda adults is 30-50;
A3. collecting egg masses: replacing the gauze at the bottom of the pot every 2 days, and shearing the spodoptera frugiperda egg blocks covered by the fuzz on the gauze taken out of the pot by using scissors and part of the gauze; putting the cut egg blocks into an incubation basin, putting two artificial feeds into the basin, wherein the positions where the egg blocks are placed are the upper surfaces of the artificial feeds; covering the incubation basin with a plastic disc to prevent larvae from escaping after eggs are incubated; incubating Spodoptera frugiperda eggs in an incubator under the conditions of 25 +/-0.5 ℃ of temperature, 65 +/-5% of relative humidity and light cycle L, D, of 0h and 24 h;
A4. larva breeding: keeping the Spodoptera frugiperda larvae to be raised in a hatching pot from egg hatching to before the Spodoptera frugiperda larvae are three years old, respectively putting the Spodoptera frugiperda larvae into glass culture dishes with the calibers of 15-20 cm for raising after the Spodoptera frugiperda larvae are three years old, putting 1-20 larvae of the Spodoptera frugiperda and three artificial feeds into each culture dish, and continuously raising until the larvae pupate; feeding Spodoptera frugiperda larvae in a culture box under the conditions of 25 +/-0.5 ℃ of temperature, 65 +/-5% of relative humidity and light cycle L, D being 0h and 24 h;
A5. collecting pupae: when the Spodoptera frugiperda pupae becomes hard and dark brown, taking the pupae out of the culture dish and putting the pupae into a plastic box until the pupae eclosion occurs; the emergence of the Spodoptera frugiperda pupae is carried out under the condition of full darkness;
A6. adult supplementation: regularly observing whether the plastic box for containing the pupae has imago eclosion or not, and connecting the newly eclosion imago into the insect feeding tank to form circulation.
2. The artificial propagation method according to claim 1, characterized in that: the artificial feed is made into single block with a length of about 5cm, a width of 3.5cm and a height of 1.5 cm.
3. The artificial propagation method according to claim 1, characterized in that: the contents of the components in the basic formula of the artificial feed can be increased and decreased in proportion according to the production amount of the feed.
4. The artificial propagation method according to claim 1, characterized in that: corn leaf powder is added into the artificial feed to improve the palatability of the feed.
5. The artificial propagation method according to claim 1, characterized in that: in the process of preparing the artificial feed, the main material, the shaping agent and the nutrient solution are uniformly mixed, the temperature is controlled to be 45-50 ℃, substances in the nutrient solution are easy to deform when the temperature is too high, and the shaping agent is easy to solidify when the temperature is too low, so that the main material, the shaping agent and the nutrient solution are not beneficial to uniform mixing.
6. The artificial propagation method according to claim 1, characterized in that: in order to enable the spodoptera frugiperda imagoes to have enough activity space, the diameter of the insect breeding tank is 35-50 cm, and the height of the insect breeding tank is 20-40 cm.
7. The artificial propagation method according to claim 1, characterized in that: when collecting the pupae of the Spodoptera frugiperda, the pupae should be fully pupated and become hard so as to avoid damaging the pupae.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911231473.1A CN110915761B (en) | 2019-12-05 | 2019-12-05 | Indoor artificial propagation method for Spodoptera frugiperda in full-dark condition in young stage |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911231473.1A CN110915761B (en) | 2019-12-05 | 2019-12-05 | Indoor artificial propagation method for Spodoptera frugiperda in full-dark condition in young stage |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110915761A true CN110915761A (en) | 2020-03-27 |
| CN110915761B CN110915761B (en) | 2021-11-26 |
Family
ID=69856800
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911231473.1A Active CN110915761B (en) | 2019-12-05 | 2019-12-05 | Indoor artificial propagation method for Spodoptera frugiperda in full-dark condition in young stage |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110915761B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111449028A (en) * | 2020-06-09 | 2020-07-28 | 贵阳学院 | Winter laboratory breeding method for Spodoptera frugiperda |
| CN111735939A (en) * | 2020-06-08 | 2020-10-02 | 仲恺农业工程学院 | Kit, special feed bottle and method for rapidly identifying drug resistance of spodoptera frugiperda |
| CN111758673A (en) * | 2020-07-16 | 2020-10-13 | 中国热带农业科学院环境与植物保护研究所 | Method for expanding propagation of spodoptera frugiperda trichogramma by preserving spodoptera frugiperda egg masses |
| CN112075390A (en) * | 2020-09-15 | 2020-12-15 | 广东省农业科学院植物保护研究所 | Spodoptera frugiperda parasitic wasp propagation method |
| CN112841459A (en) * | 2021-02-02 | 2021-05-28 | 浙江省农业科学院 | Artificial feed for promoting rapid growth and propagation of springtail and preparation method thereof |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2004295386A1 (en) * | 2003-12-01 | 2005-06-16 | Syngenta Participations Ag | Insect resistant cotton plants and methods of detecting the same |
| CN101449670A (en) * | 2007-11-30 | 2009-06-10 | 武汉亿农丰生物工程有限公司 | Yellow meal worm larva breeding method |
| CN103070309A (en) * | 2013-01-09 | 2013-05-01 | 中国热带农业科学院环境与植物保护研究所 | Prodenia litura artificial feed, preparation method thereof and method for raising prodenia litura |
| CN205667254U (en) * | 2016-06-12 | 2016-11-02 | 新疆师范大学 | A kind of Chrysopa larva raising dish |
| CN108077191A (en) * | 2017-12-29 | 2018-05-29 | 四川省农业科学院植物保护研究所 | A kind of Spodoptera litura larvae group breeding method |
| CN109965088A (en) * | 2019-03-01 | 2019-07-05 | 河南省农业科学院植物保护研究所 | Spodopterafrugiperda larva artificial feed, preparation method and application |
| CN109984098A (en) * | 2019-05-15 | 2019-07-09 | 山西农业大学 | A kind of oriental fruit months experimental population expanding propagation method |
| CN110089642A (en) * | 2019-06-15 | 2019-08-06 | 安徽省农业科学院植物保护与农产品质量安全研究所 | A kind of Spodopterafrugiperda man-made feeds |
| CN110367407A (en) * | 2019-09-02 | 2019-10-25 | 浙江省农业科学院 | A kind of Spodopterafrugiperda larva artificial feed and its preparation method and application |
-
2019
- 2019-12-05 CN CN201911231473.1A patent/CN110915761B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2004295386A1 (en) * | 2003-12-01 | 2005-06-16 | Syngenta Participations Ag | Insect resistant cotton plants and methods of detecting the same |
| CN101449670A (en) * | 2007-11-30 | 2009-06-10 | 武汉亿农丰生物工程有限公司 | Yellow meal worm larva breeding method |
| CN103070309A (en) * | 2013-01-09 | 2013-05-01 | 中国热带农业科学院环境与植物保护研究所 | Prodenia litura artificial feed, preparation method thereof and method for raising prodenia litura |
| CN205667254U (en) * | 2016-06-12 | 2016-11-02 | 新疆师范大学 | A kind of Chrysopa larva raising dish |
| CN108077191A (en) * | 2017-12-29 | 2018-05-29 | 四川省农业科学院植物保护研究所 | A kind of Spodoptera litura larvae group breeding method |
| CN109965088A (en) * | 2019-03-01 | 2019-07-05 | 河南省农业科学院植物保护研究所 | Spodopterafrugiperda larva artificial feed, preparation method and application |
| CN109984098A (en) * | 2019-05-15 | 2019-07-09 | 山西农业大学 | A kind of oriental fruit months experimental population expanding propagation method |
| CN110089642A (en) * | 2019-06-15 | 2019-08-06 | 安徽省农业科学院植物保护与农产品质量安全研究所 | A kind of Spodopterafrugiperda man-made feeds |
| CN110367407A (en) * | 2019-09-02 | 2019-10-25 | 浙江省农业科学院 | A kind of Spodopterafrugiperda larva artificial feed and its preparation method and application |
Non-Patent Citations (6)
| Title |
|---|
| YUKINORI MUKAI 等: "Reduced cannibalistic behavior of African catfish, Clarias gariepinus, larvae under dark and dim conditions", 《ZOOLOGICAL SCIENCE》 * |
| 杨明进 等: "《宁夏水稻主要病虫草害识别与防治》", 31 December 2018, 阳光出版社 * |
| 王熠 等: "光周期对黏虫生长发育和生殖的影响", 《植物保护学报》 * |
| 王铁生: "《中国人参》", 31 December 2001, 辽宁科学技术出版社 * |
| 赵胜园 等: "草地贪夜蛾与斜纹夜蛾的形态特征和生物学习性比较", 《中国植保导刊》 * |
| 金明弟 等: "《蔬菜职业农民技术指南》", 31 May 2018, 上海科学技术出版社 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111735939A (en) * | 2020-06-08 | 2020-10-02 | 仲恺农业工程学院 | Kit, special feed bottle and method for rapidly identifying drug resistance of spodoptera frugiperda |
| CN111449028A (en) * | 2020-06-09 | 2020-07-28 | 贵阳学院 | Winter laboratory breeding method for Spodoptera frugiperda |
| CN111758673A (en) * | 2020-07-16 | 2020-10-13 | 中国热带农业科学院环境与植物保护研究所 | Method for expanding propagation of spodoptera frugiperda trichogramma by preserving spodoptera frugiperda egg masses |
| CN111758673B (en) * | 2020-07-16 | 2021-11-23 | 中国热带农业科学院环境与植物保护研究所 | Method for expanding propagation of spodoptera frugiperda trichogramma by preserving spodoptera frugiperda egg masses |
| CN112075390A (en) * | 2020-09-15 | 2020-12-15 | 广东省农业科学院植物保护研究所 | Spodoptera frugiperda parasitic wasp propagation method |
| CN112841459A (en) * | 2021-02-02 | 2021-05-28 | 浙江省农业科学院 | Artificial feed for promoting rapid growth and propagation of springtail and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110915761B (en) | 2021-11-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110915761B (en) | Indoor artificial propagation method for Spodoptera frugiperda in full-dark condition in young stage | |
| CN100388884C (en) | Method for breeding pyemote, and its culturing vessel, and method for releasing same | |
| CN102356811B (en) | Formula of feed for chilo suppressalis artificial feeding, preparation method of the feed, and large-scale feeding method of chilo suppressalis | |
| CN101796940A (en) | Super flour weevil culture technology | |
| CN112868606B (en) | Method for indoor raising sugarcane striped rice borers or dibbler | |
| CN110583914A (en) | Spodoptera frugiperda semi-artificial feed and preparation method and feeding method thereof | |
| CN101810154A (en) | Method for industrially producing zophobas morio | |
| CN105746435A (en) | Method for propagating chrysopa pallens larvae through rice moth eggs | |
| CN110916022A (en) | Artificial feed for spodoptera frugiperda and feeding method | |
| CN108813114B (en) | Artificial feed for corn borer larvae as well as preparation method and application thereof | |
| CN112602860A (en) | Artificial feed for feeding wide-band excellent aphids and preparation method and application thereof | |
| CN102578432B (en) | Artificial feed preparation and simplified feeding method for Athelis lepigone larva | |
| CN110179016A (en) | A kind of man-made feeds of Spodopterafrugiperda and preparation method thereof | |
| CN110169492B (en) | Artificial feed for harmonia axyridis larvae as well as preparation method and application of artificial feed | |
| CN114651793B (en) | Artificial breeding of trichogramma obscura and method for preventing and treating spodoptera frugiperda by using trichogramma obscura | |
| CN109198308B (en) | Special feed for artificial feeding of coffee moth larvae and artificial feeding method thereof | |
| CN103478486A (en) | Diamondback moth larva pure artificial feed and preparation method thereof | |
| CN112471082B (en) | Broadband preferred aphid breeding method | |
| CN111066732B (en) | Method for breeding orius sauteri | |
| CN111789202A (en) | Artificial feed for fall webworms and preparation method and application thereof | |
| CN113100358A (en) | Artificial feed for promoting growth of lepidoptera insects | |
| CN112471368A (en) | Artificial feed for cowpea pod borer larvae and preparation method thereof | |
| CN108142382B (en) | Method for breeding dichocrocis punctiferalis on large scale | |
| CN102835596B (en) | Artificial feed of proxenus lepigone larva | |
| CN116982681B (en) | Spodoptera frugiperda artificial feed and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |