CN112841459A - Artificial feed for promoting rapid growth and propagation of springtail and preparation method thereof - Google Patents
Artificial feed for promoting rapid growth and propagation of springtail and preparation method thereof Download PDFInfo
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- CN112841459A CN112841459A CN202110140251.XA CN202110140251A CN112841459A CN 112841459 A CN112841459 A CN 112841459A CN 202110140251 A CN202110140251 A CN 202110140251A CN 112841459 A CN112841459 A CN 112841459A
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- feed
- springtail
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- propagation
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- 229930182817 methionine Natural products 0.000 claims abstract description 11
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 claims abstract description 9
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 claims abstract description 9
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- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 claims abstract description 7
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- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
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- A—HUMAN NECESSITIES
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- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
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- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
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- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
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- A—HUMAN NECESSITIES
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- A23K20/163—Sugars; Polysaccharides
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- A23K20/00—Accessory food factors for animal feeding-stuffs
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- A23K20/174—Vitamins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/22—Compounds of alkali metals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Inorganic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Birds (AREA)
- Mycology (AREA)
- Physiology (AREA)
- Insects & Arthropods (AREA)
- Fodder In General (AREA)
Abstract
The invention relates to an artificial feed for promoting rapid growth and reproduction of springtail, which comprises the following components in percentage by weight: 2-5 g/L of essential amino acid, 200g/L of protein, 2-5 g/L of vitamin, 1-4 g/L of dipotassium phosphate, 0.1-2 g/L of magnesium sulfate heptahydrate, 0.1-2 g/L of potassium chloride, 0.01-1 g/L of ferrous sulfate, 15-25 g/L of agar and the balance of water; wherein the essential amino acid is any one of lysine, tryptophan or methionine, the protein is radix Apioris Fortunei powder or bean powder, and the vitamin is vitamin C and/or vitamin E. The invention also relates to a preparation method and a using method of the feed. The feed provided by the invention greatly improves the growth speed and biomass of the springtail, the springtail can be bred into one generation after being cultured for 7d, the quantity can reach more than 600, and the requirement of actual research work of biology and entomology on the quantity of the springtail can be fully met.
Description
Technical Field
The invention belongs to the field of microorganisms, and particularly relates to an artificial feed for promoting rapid growth and propagation of springtail and a preparation method thereof.
Background
The Phyllostachys Pubescens (Hypogastrorea communis) belongs to the Phyllotreta (Collembola) family (Hypogastrorea), is the most common harmful species on mushroom beds, often floats on water surface in a large scale and is called black purple, mainly harms edible fungi such as oyster mushroom, straw mushroom and mushroom, and sometimes also harms vegetables such as iceberg and the like. The purple springtail usually propagates and lives on fertile and moist soil, weeds, occasional roads in a storehouse and animal manure, and is visible all the year round.
At present, there are many varieties of artificial insect feeds, and different insect varieties have different feed formulas, such as carposina persicae, corn borer, prodenia litura, spodoptera exigua and the like, however, although the artificial feed formulas of the insects can propagate the springtail insects, the quantity of the obtained insects is still small, and can not reach the number of the experiments, and the requirements of rapidly growing and propagating the springtail insects can not be met at all, so that the accurate screening experiment for researching the medicament of the insects is difficult to complete, and the application of the springtail insects in the aspects of biological characteristics and related researches of entomology of underground rhizome crops is severely restricted. Therefore, innovations in feed formulations and feeding modes of springtails are needed in order to obtain high-yield springtails.
Disclosure of Invention
The invention aims to provide an artificial feed for promoting the rapid growth and propagation of springtails, which solves the problems that although the conventional insect artificial feed formula can propagate the springtails, the number of the obtained insects is still small, the number of the insects can not reach the number of the insects to be tested, and the requirements for the rapid growth and propagation of the springtails can not be met at all.
The second purpose of the invention is to provide a preparation method of the artificial feed for promoting the rapid growth and reproduction of the springtail insects.
The second purpose of the invention is to provide a use method of the artificial feed for promoting the rapid growth and reproduction of the springtail insects.
The invention is realized by the following technical scheme:
an artificial feed for promoting rapid growth and propagation of springtails comprises the following components in percentage by weight: 2-5 g/L of essential amino acid, 200g/L of protein, 2-5 g/L of vitamin, 1-4 g/L of dipotassium phosphate, 0.1-2 g/L of magnesium sulfate heptahydrate, 0.1-2 g/L of potassium chloride, 0.01-1 g/L of ferrous sulfate, 15-25 g/L of agar and the balance of water; wherein the essential amino acid is any one of lysine, tryptophan or methionine, the protein is radix Apioris Fortunei powder or bean powder, and the vitamin is vitamin C and/or vitamin E.
Further, the components and contents are as follows: 5g/L of essential amino acid, 200g/L of protein, 5g/L of vitamin, 1g/L of dipotassium phosphate, 0.5g/L of magnesium sulfate heptahydrate, 0.5g/L of potassium chloride, 0.01g/L of ferrous sulfate, 20g/L of agar and the balance of water; wherein the essential amino acid is any one of lysine, tryptophan or methionine, the protein is radix Apioris Fortunei powder or bean powder, and the vitamin is vitamin C and/or vitamin E.
Further, the pH value of the feed is 7-8.
Further, the pH value of the feed is 7.
Further, the type of the springtail is springtail (Hypogastroura communis).
Secondly, accurately weighing the essential amino acids, proteins, vitamins, dipotassium hydrogen phosphate, magnesium sulfate heptahydrate, potassium chloride, ferrous sulfate and agar according to the formula amount, mixing together, adding water to 1000mL, and sterilizing at high temperature to obtain the artificial feed for promoting the rapid growth and propagation of the springtails.
Further, the high-temperature sterilization temperature is 121 ℃, and the time is 30 min.
And thirdly, according to the using method of the artificial feed for promoting the rapid growth and propagation of the springtail, the specific feed and the rice bran are mixed according to the weight ratio of 1:1 for use.
Furthermore, the specific feed is prepared and then crushed into particles with the size of 8-15mm multiplied by 8-15mm by a crusher, and then the particles are mixed with rice bran according to the weight ratio of 1:1 for use.
Adopt above-mentioned technical scheme's positive effect: (1) the growth speed and biomass of the springtail are greatly improved due to the specific essential amino acids, proteins and vitamins suitable for the growth and propagation of the springtail, the springtail can be propagated for one generation after being cultured for 7 days, and the number of the springtail can reach more than 600, so that powerful support is provided for the next step of experiments of morphological observation, biological characteristics, physiological characteristics, medicament control screening and the like of the pests;
(2) the invention increases the reproduction quantity of the springtail, rapidly grows by skillfully utilizing the mixing of the rice bran and the solid feed on the premise of not influencing the activity of the springtail, can obtain a large number of worm bodies within 7 days, and can fully meet the requirement of actual research work of biology and entomology on the quantity of the springtail.
Detailed Description
The technical solution of the present invention is further described below with reference to specific examples and test examples, but the present invention should not be construed as being limited thereto.
In the present invention, unless otherwise specified, the methods used in the examples are techniques commonly used in the art, and all the equipment, raw materials and the like are products commonly used in the industry and are commercially available. The type of the springtail is the purple springtail (Hypogastroura communis) in the invention, and the field is collected and raised by the plant protection microorganism research institute of agricultural academy of sciences in Zhejiang province.
Example 1
This example illustrates the preparation of a feed.
The method comprises the following steps: 1g of dipotassium hydrogen phosphate, 0.5g of potassium chloride and 7 g of magnesium sulfate hydrate (MgSO)4·7H2O)0.5g, ferrous sulfate 0.01g, tryptophan 3g, bean flour 200g, vitamin C3 g, sucrose 30g and agar 20 g;
step two: mixing the weighed medicines together, and adding deionized water to a constant volume of 1000 mL;
step three: placing the prepared feed into an autoclave, sterilizing at 121 deg.C under high pressure for 30min, and taking out;
step four: the sterilized solid feed is put into a grinder to be ground into the size of 8-15mm multiplied by 8-15mm, and the ground solid feed is mixed with rice bran according to the weight ratio of 1:1 and then is put into a culture tank.
Example 2
This example illustrates the preparation of a feed.
The method comprises the following steps: 1g of dipotassium hydrogen phosphate, 0.5g of potassium chloride and 7 g of magnesium sulfate hydrate (MgSO)4·7H2O)0.5g, ferrous sulfate 0.01g, lysine 3g, radix tetrastigme powder 200g, vitamin C1.5g, vitamin E1.5g, sucrose 30g and agar 20 g;
step two: mixing the weighed medicines together, and adding deionized water to a constant volume of 1000 mL;
step three: placing the prepared feed into an autoclave, sterilizing at 121 deg.C under high pressure for 30min, and taking out;
step four: the sterilized solid feed is put into a grinder to be ground into the size of 8-15mm multiplied by 8-15mm, and the ground solid feed is mixed with rice bran according to the weight ratio of 1:1 and then is put into a culture tank.
Example 3
This example illustrates the preparation of a feed.
The method comprises the following steps: 1g of dipotassium hydrogen phosphate, 0.5g of potassium chloride and 7 g of magnesium sulfate hydrate (MgSO)4·7H2O)0.5g, ferrous sulfate 0.01g, methionine 3g, bean flour 200g, vitamin E3 g, sucrose 30g, agar 20 g;
step two: mixing the weighed medicines together, and adding deionized water to a constant volume of 1000 mL;
step three: placing the prepared feed into an autoclave, sterilizing at 121 deg.C under high pressure for 30min, and taking out;
step four: the sterilized solid feed is put into a grinder to be ground into the size of 8-15mm multiplied by 8-15mm, and the ground solid feed is mixed with rice bran according to the weight ratio of 1:1 and then is put into a culture tank.
Example 4
This example illustrates the preparation of a feed.
The method comprises the following steps:1g of dipotassium hydrogen phosphate, 0.1g of potassium chloride and 7 g of magnesium sulfate hydrate (MgSO)4·7H2O)0.1g, ferrous sulfate 0.01g, methionine 2g, bean flour 200g, vitamin C2 g, sucrose 30g, agar 15 g;
step two: mixing the weighed medicines together, and adding deionized water to a constant volume of 1000 mL;
step three: placing the prepared feed into an autoclave, sterilizing at 121 deg.C under high pressure for 30min, and taking out;
step four: the sterilized solid feed is put into a grinder to be ground into the size of 8-15mm multiplied by 8-15mm, and the ground solid feed is mixed with rice bran according to the weight ratio of 1:1 and then is put into a culture tank.
Example 5
This example illustrates the preparation of a feed.
The method comprises the following steps: potassium phosphate dibasic 4g, potassium chloride 2g, magnesium sulfate hydrate 7 g (MgSO)4·7H2O)2g, 1g of ferrous sulfate, 5g of methionine, 200g of radix tetrastigme powder, 5g of vitamin E, 30g of sucrose and 25g of agar;
step two: mixing the weighed medicines together, and adding deionized water to a constant volume of 1000 mL;
step three: placing the prepared feed into an autoclave, sterilizing at 121 deg.C under high pressure for 30min, and taking out;
step four: the sterilized solid feed is put into a grinder to be ground into the size of 8-15mm multiplied by 8-15mm, and the ground solid feed is mixed with rice bran according to the weight ratio of 1:1 and then is put into a culture tank.
Test example 1
This test example illustrates the effect of feed with different essential amino acids on the reproductive growth of springtail.
In this example, the effect of different nitrogen sources on the propagation and growth of springtail was investigated by adding different essential amino acids based on solid czochralski medium.
The components of the Chaudhur culture medium are as follows: dipotassium hydrogen phosphate 1g, potassium chloride 0.5g, magnesium sulfate hydrate (MgSO)4·7H2O)0.5g, ferrous sulfate 0.01g, potassium nitrate 3g, sucrose 30g and agar 20 g.
Lysine, tryptophan, methionine, glycine, glutamic acid, phenylalanine, proline, tyrosine, leucine and isoleucine are respectively used for replacing potassium nitrate in the Chaudou culture medium, so that other components in each feed are inconvenient to maintain, and the feed with 10 different nitrogen sources is prepared.
The preparation method of the feed and the feeding method of the springtail comprise the following steps:
the method comprises the following steps: 1g of dipotassium hydrogen phosphate, 0.5g of potassium chloride and 7 g of magnesium sulfate hydrate (MgSO)4·7H2O)0.5g, ferrous sulfate 0.01g, vitamin 3g, sucrose 30g, agar 20 g;
step two: mixing the weighed medicines together, and adding deionized water to a constant volume of 1000 mL;
step three: placing the prepared feed into an autoclave, sterilizing at 121 deg.C under high pressure for 30min, and taking out;
step four: putting the sterilized solid feed into a grinder, grinding to 8-15mm multiplied by 8-15mm, and putting the ground solid feed into a culture tank for later use;
step five: pouring the collected springtails into a culture tank, carrying out shading feeding, and counting the number of heads and the number of eggs of the springtails in the culture tank after 15 days;
TABLE 1 reproduction and growth of springtail with different essential amino acid feeds
As can be seen from the data in Table 1, different nitrogen sources have a greater effect on the growth of springtail. For different nitrogen sources, experiments show that the springtail grows relatively fast and has large breeding amount in feeds taking lysine, tryptophan and methionine as nitrogen sources, but grows relatively slow and has limited breeding in feeds taking glycine, glutamic acid and phenylalanine as nitrogen sources, but basically does not breed in feeds taking proline, tyrosine, leucine, isoleucine and potassium nitrate, and the springtail for test dies. In general, suitable nitrogen sources for the growth and reproduction of springtail are lysine, tryptophan, methionine.
Test example 2
This test example illustrates the effect of different protein feeds on the reproductive growth of springtail.
Preparation and springtail culture of optimized feed taking lysine as nitrogen source and sucrose as carbon source
The method comprises the following steps: 1g of dipotassium hydrogen phosphate, 0.5g of potassium chloride and 7 g of magnesium sulfate hydrate (MgSO)4·7H2O)0.5g, ferrous sulfate 0.01g, lysine 3g, sucrose 30g, agar 20 g;
step two: mixing the weighed medicines together, adding deionized water to a constant volume of 1000mL, and adjusting the pH to 7;
step three: placing the prepared feed into an autoclave, sterilizing at 121 deg.C under high pressure for 30min, and taking out;
step four: putting the sterilized solid feed into a grinder, grinding to 8-15mm multiplied by 8-15mm, and putting the ground solid feed into a culture tank for later use;
step five: pouring the collected springtails into a culture tank, carrying out shading feeding, and counting the number of heads and the number of eggs of the springtails in the culture tank after 15 days;
(II) preparation of optimized feed of rice flour and bean flour and springtail culture
The method comprises the following steps: 1g of dipotassium hydrogen phosphate, 0.5g of potassium chloride and 7 g of magnesium sulfate hydrate (MgSO)4·7H2O)0.5g, ferrous sulfate 0.01g, lysine 3g and agar 20 g;
step two: respectively adding 200g of rice flour, 200g of bean flour, 200g of radix tetrastigme powder and 30g of cane sugar into the feed medicine in the first step;
step three: adding deionized water into the feed to a constant volume of 1000mL, adding agar, stirring uniformly, adjusting the pH to 7, and respectively preparing a rice feed, a bean powder feed and a radix tetrastigme powder feed;
step three: placing the prepared different types of feed into an autoclave, sterilizing at 121 deg.C for 30min, and taking out;
step four: putting the sterilized solid feed into a grinder, grinding to 8-15mm multiplied by 8-15mm, and putting the ground solid feed into a culture tank for later use;
step five: pouring the collected springtails into a culture tank, carrying out shading feeding, and counting the number of heads and the number of eggs of the springtails in the culture tank after 15 days;
TABLE 2 reproduction and growth of springtail with different protein feeds
As can be seen from the data in Table 2, different proteins have a greater effect on the growth of springtail. For different proteins, experiments show that the springtail grows relatively fast and produces a large amount of feed in the feed of the radix tetrastigme powder and the bean powder, but grows relatively slow and produces limited feed in the feed of the rice powder and the cane sugar. In general, the proteins suitable for the growth and reproduction of springtail are radix tetrastigme powder and bean powder.
Test example 3
This test example illustrates the effect of an optimized feed of different vitamins on the reproduction and growth of springtail.
The preparation method of the feed is the same as that of the experimental example 2, different vitamins are added into different feeds, the prepared different types of feeds are placed in an autoclave, and the feeds are taken out for standby after being autoclaved at 121 ℃ for 30 min; then putting the sterilized solid feed into a grinder, grinding to 8-15mm multiplied by 8-15mm, and putting the ground solid feed into a culture tank for later use; and finally, pouring the collected springtails into a culture tank, and raising the springtails in a shading mode.
The measurement results are shown in table 3, in which a 1: soybean flour lysine feed; a2: radix tetrastigme lysine feed; a3: soybean flour lysine feed +3g vitamin C; a4: radix tetrastigme lysine feed +3g vitamin C; a5: soybean flour lysine feed +3g vitamin E; a6: radix tetrastigme lysine feed +3g vitamin E; a7: soybean flour lysine feed +1.5g vitamin C +1.5g vitamin E; a8: the feed is prepared from radix tetrastigme lysine, 1.5g vitamin C and 1.5g vitamin E, the feeding temperature is 26 ℃, the number of heads and eggs of springtails in the culture tank are counted after continuous culture is carried out for 15 days, and the numerical value in the table is an average value of 3 times of repetition.
TABLE 3 reproduction and growth of springtails in different vitamin-supplemented feeds
As can be seen from the data in table 3, the addition of different vitamins has a greater effect on the growth status of the springtail. For different vitamins, experiments show that the springtail grows relatively fast and has large reproduction amount in the feed containing the radix tetrastigme powder and the bean powder, but the feed added with the vitamin C and the vitamin E can obviously accelerate the reproduction capability of the springtail, the reproduction capability of the springtail is doubled and the number of eggs is increased in the feed added with the vitamin C and the vitamin E at the same time. Therefore, the addition of vitamin C and vitamin E is easier for the growth and reproduction of the springtail.
Test example 4
This test example illustrates the effect of different feed mix types on the reproduction and growth of springtail.
Preparation of Chaochi formula feed added with lysine, radix tetrastigme powder and vitamins and springtail feeding
The method comprises the following steps: 1g of dipotassium hydrogen phosphate, 0.5g of potassium chloride and 7 g of magnesium sulfate hydrate (MgSO)4·7H2O)0.5g, ferrous sulfate 0.01g, lysine 3g, radix tetrastigme powder 200g, vitamin C1.5g, vitamin E1.5g, agar 20 g;
step two: mixing the weighed medicines together, adding deionized water to a constant volume of 1000mL, and adjusting the pH to 7;
step three: placing the prepared optimized feed into an autoclave, sterilizing at 121 deg.C for 30min, and taking out;
step four: putting the sterilized solid feed into a grinder, grinding to 8-15mm multiplied by 8-15mm, and putting the ground solid feed into a culture tank for later use;
step five: pouring the collected springtails into a culture tank, carrying out shading feeding, and counting the number of heads and the number of eggs of the springtails in the culture tank after 15 days;
(II) preparation of optimized feed added with rice bran and springtail feeding
Step (ii) ofFirstly, the method comprises the following steps: 1g of dipotassium hydrogen phosphate, 0.5g of potassium chloride and 7 g of magnesium sulfate hydrate (MgSO)4·7H2O)0.5g, ferrous sulfate 0.01g, lysine 3g, radix tetrastigme powder 200g, vitamin C1.5g, vitamin E1.5g, agar 20 g;
step two: mixing the weighed medicines together, adding deionized water to a constant volume of 1000mL, and adjusting the pH to 7;
step three: placing rice bran and the prepared optimized feed into an autoclave, sterilizing at 121 ℃ for 30min under high pressure, and taking out for later use;
step four: putting the sterilized solid feed into a grinder, grinding to 8-15mm multiplied by 8-15mm, mixing the ground solid feed and rice bran according to the proportion of 1:1, 1:2 and 2:1, and placing the mixture in a culture tank for later use;
step five: pouring the collected springtails into a culture tank, carrying out shading feeding, and counting the number of heads and the number of eggs of the springtails in the culture tank after 15 days;
the measurement results are shown in table 4, in which a 1: the feed of the invention; a2: mixing the crushed solid feed and rice bran according to the proportion of 1: 2; a3: mixing the crushed solid feed and rice bran according to a ratio of 2: 1; a4: the crushed solid feed is cultured at the temperature of 26 ℃ for 15d continuously, and then the number of heads and eggs of the springtail in the culture tank are counted, and the numerical value in the table is the average value of 3 times of repetition.
TABLE 4 Effect of different feed mix types on the reproduction and growth of springtails
As can be seen from the results in table 4, different feed mix types had a greater effect on the growth conditions of the springtail. For the feed element mixed with the rice bran in different proportions, the propagation speed of the springtails can be obviously increased, the distance between feeds can be increased due to the increase of the rice bran, and the activity and spawning sites for the springtails are better provided. Experiments have found that the springtail grows relatively fast and breeds a lot in the feed mixed with the pulverized solid feed and the rice bran in a ratio of 1:1, while the springtail has a relatively weak breeding capacity in the feed mixed with the pulverized solid feed and the rice bran in a ratio of 1:2 and 2: 1. Therefore, the feed mixed by the crushed solid feed and the rice bran according to the ratio of 1:1 is easier to grow and propagate the springtails.
The terms and expressions which have been employed herein as terms of description and not of limitation are not intended to be exclusive and there is no intention in the use of such terms and expressions of excluding any equivalents of the features shown and described or their equivalents, but it is recognized that various modifications are possible within the scope of the invention claimed. Thus, it is believed that while the invention has been set forth herein in a variety of specific embodiments and with any features described in detail, it is believed that alterations to the design disclosed herein will be resorted to those skilled in the art, and that such alterations will be consistent with the appended claims. The contents of the articles, patents, patent applications, and all other documents, and the useful electronic information mentioned and cited herein are incorporated by reference in their entirety, to the extent any portion of this disclosure is specifically identified. The applicants have the right to incorporate into this application any and all information and material from such articles, patents, patent applications, or other documents as part of the disclosure of this patent specification.
The growth speed and biomass of the springtail are greatly improved due to the specific essential amino acids, proteins and vitamins suitable for the growth and propagation of the springtail, the springtail can be propagated for one generation after being cultured for 7 days, and the number of the springtail can reach more than 600, so that powerful support is provided for the next step of experiments of morphological observation, biological characteristics, physiological characteristics, medicament control screening and the like of the pests;
the invention increases the reproduction quantity of the springtail, rapidly grows by skillfully utilizing the mixing of the rice bran and the solid feed on the premise of not influencing the activity of the springtail, can obtain a large number of worm bodies within 7 days, and can fully meet the requirement of actual research work of biology and entomology on the quantity of the springtail.
Claims (9)
1. An artificial feed for promoting rapid growth and reproduction of springtails, which is characterized in that: the components and contents are as follows: 2-5 g/L of essential amino acid, 200g/L of protein, 2-5 g/L of vitamin, 1-4 g/L of dipotassium phosphate, 0.1-2 g/L of magnesium sulfate heptahydrate, 0.1-2 g/L of potassium chloride, 0.01-1 g/L of ferrous sulfate, 15-25 g/L of agar and the balance of water; wherein the essential amino acid is any one of lysine, tryptophan or methionine, the protein is radix Apioris Fortunei powder or bean powder, and the vitamin is vitamin C and/or vitamin E.
2. The artificial feed for promoting rapid growth and propagation of springtail according to claim 1, wherein the artificial feed comprises: the components and contents are as follows: 5g/L of essential amino acid, 200g/L of protein, 5g/L of vitamin, 1g/L of dipotassium phosphate, 0.5g/L of magnesium sulfate heptahydrate, 0.5g/L of potassium chloride, 0.01g/L of ferrous sulfate, 20g/L of agar and the balance of water; wherein the essential amino acid is any one of lysine, tryptophan or methionine, the protein is radix Apioris Fortunei powder or bean powder, and the vitamin is vitamin C and/or vitamin E.
3. The artificial feed for promoting rapid growth and propagation of springtail as claimed in claim 1 or 2, wherein: the pH value of the feed is 7-8.
4. The artificial feed for promoting rapid growth and reproduction of springtails according to claim 3, wherein: the pH value of the feed is 7.
5. The artificial feed for promoting rapid growth and propagation of springtail as claimed in claim 1 or 2, wherein: the type of the springtail is purple springtail (Hypogastroura communis).
6. A method for preparing the artificial feed for promoting the rapid growth and propagation of the springtail insects according to any one of claims 1 to 5, which is characterized by comprising the following steps: accurately weighing essential amino acids, proteins, vitamins, dipotassium hydrogen phosphate, magnesium sulfate heptahydrate, potassium chloride, ferrous sulfate and agar according to the formula ratio, mixing together, adding water to 1000mL, and sterilizing at high temperature to obtain the feed.
7. The method of claim 7, wherein: the high-temperature sterilization temperature is 121 ℃, and the time is 30 min.
8. The use method of the artificial feed for promoting the rapid growth and propagation of the springtail insects according to any one of claims 1 to 5, is characterized in that: the specific feed and the rice bran are mixed according to the weight ratio of 1:1 for use.
9. Use according to claim 8, characterized in that: the specific feed is prepared into particles with the size of 8-15mm multiplied by 8-15mm by a pulverizer, and the particles are mixed with rice bran according to the weight ratio of 1:1 for use.
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| NL2030624A NL2030624B1 (en) | 2021-02-02 | 2022-01-20 | Artificial Feed for Accelerating Rapid Growth and Reproduction of Collembolan and Preparation Method Thereof |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102415358A (en) * | 2011-09-14 | 2012-04-18 | 中国科学院东北地理与农业生态研究所 | Moisture-preserving springtail culture matrix and preparation method thereof |
| CN106721677A (en) * | 2017-01-04 | 2017-05-31 | 中国科学院沈阳应用生态研究所 | A kind of springtail implantation nutrient solution and preparation method thereof |
| CN110915761A (en) * | 2019-12-05 | 2020-03-27 | 山西农业大学 | Indoor artificial propagation method for Spodoptera frugiperda in full-dark condition in young stage |
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- 2021-02-02 CN CN202110140251.XA patent/CN112841459A/en active Pending
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102415358A (en) * | 2011-09-14 | 2012-04-18 | 中国科学院东北地理与农业生态研究所 | Moisture-preserving springtail culture matrix and preparation method thereof |
| CN106721677A (en) * | 2017-01-04 | 2017-05-31 | 中国科学院沈阳应用生态研究所 | A kind of springtail implantation nutrient solution and preparation method thereof |
| CN110915761A (en) * | 2019-12-05 | 2020-03-27 | 山西农业大学 | Indoor artificial propagation method for Spodoptera frugiperda in full-dark condition in young stage |
Non-Patent Citations (2)
| Title |
|---|
| 尹钢: "《昆虫》", 30 April 2013, 北京工业大学出版社, pages: 175 - 177 * |
| 赵岩: "跳虫培养瓶和培养基的选用及优化", 《湖南农业科学》, 31 December 2011 (2011-12-31), pages 89 - 90 * |
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