KR101719773B1 - Feed containing Paecilomyces tenuipes for Protaetia spp. and method of producing the same - Google Patents

Abstract

The present invention relates to feeds of flowering larva larvae containing Cordyceps sinensis and a method for producing the same. Using the feed of the present invention and the method of producing the same, it is possible to produce a flowering larva having an increased number of individuals and an increased immunity. Therefore, it can greatly contribute to productivity improvement and farm income of domestic larvae larvae farmers by maximizing the number of individuals while keeping the flowering larvae more healthy.

Description

Technical Field [0002] The present invention relates to a method of producing a feed of Paecilomyces tenuipes for Protaetia spp. and method of producing the same}

The present invention relates to feeds of flowering larva larvae containing Cordyceps sinensis and a method for producing the same.

The flowers are classified into four stages of egg, larva, pupa, and adult, among which the larval stage is 1, 2 and 3, the number of days is 11, 19 and 38 days, (9.7 to 88.3, 99.7 to 503.6, and 529.9 to 2242.8 mg, respectively) (Kim et al ., Korean J. Appl. Entomol. 2002, 41 (1): 43-47).

The larvae larvae used in domestic insect farms are completed by adding several nutrient sources based on sawdust materials. Most farmers use their own feeds, and there is a wide variety of sawdust and nutrient sources used for each feeder. Nutrient sources include compost, nutritious sawdust with added protein, pulpwood and livestock manure. Since there is no standardized information on the method of producing nutrient source, the quality of the nutrient is different according to experience or convention. Among the individuals, there is a large difference in quality per farm, and even within the same province, the weight per individual shows a large difference. Particularly, when animal manure is used, the quality of feed and hygiene are not good. Therefore, there is a case where all the insects are killed due to damage of mite, pest damage and fungus (rust). Therefore, it is urgent to develop appropriate feeds to maximize the weight gain while keeping the domestic flower larvae uniformly and disease free.

Accordingly, the present inventors have completed the present invention by developing a flower larva larva having an enhanced immunity and promoting growth and development.

Kim et al., Korean J. Appl. Entomol. 2002. 41 (1): 43-47

It is an object of the present invention to provide a feed of a flowering larva of a flower containing Cordyceps.

It is a further object of the present invention to provide a method for producing feeds of flowering larvae.

The present invention relates to a method for producing < RTI ID = 0.0 & lt; RTI ID = 0.0 > ( Protaetia spp. ) < / RTI >

As used herein, the term "flowers ignorance flow (Protaetia spp . ) Is an insect species belonging to the beetle chafer. It is a kind of insects such as spotted flowers, white spotted flowers, plain yellow flowers, manchurial spotted flowers, plain flowers, plain flowers, The developmental stage of the flower is through the stages of eggs, larvae, pupae, and adults, and the larval stage indicates the developmental process in 1, 2, and 3 days. 41 (1): 43-47 (2002)). The present invention relates to the use of the present invention as an active ingredient, Is intended to develop larval feeds among flowering plants.

The Cordyceps can be prepared by inoculating and cultivating Cordyceps mushroom on brown rice, but the Cordyceps can be prepared by a method known to those skilled in the art. In one embodiment of the present invention, the Chinese cabbage fungus was inoculated into raw brown rice and cultured. Specifically, distilled water was added to the raw brown rice and sterilized, and then Paecilomycycles Tenuipes ) were inoculated and cultured.

As used herein, the term "caterpillar fungus" is a small mushroom of the caterpillar fungus Cryptococcus spp. And most of the insects are parasitic and produce fruiting bodies in the body of insects that become host. The insects that become host are the lepidoptera, the lepidoptera, the lumberjack, and the beetle neck.

The feed may further include, but is not limited to, a mixed fermentation product of sawdust and nutrient source. In one embodiment of the present invention, the caterpillar fungus prepared by culturing the mixed fermented product obtained by mixing the sawdust and the nutrient source is added to prepare nutritional sawdust containing Cordyceps.

The sawdust can be used both as a feedstock for the flowering larva and as a feed. Sawdust can be any kind of sawdust that can be inhabited by flowering larvae, but it can be preferably oak sawdust.

The nutrient source may include all the nutrients that can be used as a feed for the flowering larva, preferably one or more nutrients selected from the group consisting of brown sugar, rice bran, soybean, and water.

The mixed fermented product of the sawdust and the nutrient source may be prepared by mixing 0.1 to 1.0% by weight of brown sugar, 1.0 to 5.0% by weight of rice bran, 1.0 to 5.0% by weight of soybean, and 30 to 40% . In one embodiment of the present invention, the mixed fermented product was fermented by mixing 60 kg of raw oats, 375 g of brown sugar, 1.5 kg of rice bran, 2 kg of soybean, and 20 L of water (Table 1). Insects have diverse nutritional requirements. Among them, rice bran and soybean were mixed as protein sources, and cheaper brown sugar was added to supply energy source carbohydrates.

The above-mentioned Chinese cabbage is inoculated into brown rice and cultured in brown rice. The brown rice in which the Chinese cabbage has been inoculated may be contained in an amount of 1 to 10 parts by weight, preferably 5 parts by weight, based on 100 parts by weight of the mixed fermented product. Not limited.

The brown rice inoculated with the Cordyceps is in the range of 0.0001 to 10 parts by weight, preferably 0.0001 to 1 part by weight, preferably 0.0001 to 0.1 part by weight, preferably 0.0001 to 0.01 part by weight, based on 100 parts by weight of sterilized brown rice, , And most preferably 0.001 to 0.01 part by weight of the composition.

When the feed of the flower infant larva containing the Cordyceps sinensis according to the present invention is fed, the number of the larvae of the flowering larva can be increased and the immunity can be increased.

In one embodiment of the present invention, the nutritional components such as crude fat and vitamins were abundant as a result of analysis of components of nutritional sawdust feed containing cordyceps, and it was found that bacteria and heavy metals such as Escherichia coli and Salmonella were not detected, (Table 2 to Table 4).

In another embodiment of the present invention, feeding the nutritional sawdust feedstuff containing Cordyceps sinensis to the larvae of the flowers and confirming the population of the larvae showed the effect of promoting the growth, and thus the productivity of the larvae of the flowers was improved (Table 6).

In another embodiment of the present invention, the quality of nutritional sawdust feedstuffs containing Cordyceps was evaluated to show excellent quality in terms of survival rate, skin color, larval odor, etc. (Tables 7 to 9).

In addition,

1) preparing a mixed fermentation product of sawdust and nutrient source;

2) culturing the Chinese cabbage fungus; And

3) flow flower solid (Protaetia comprising the step of putting the Cordyceps sinensis strain cultured in a mixed fermented product of step 1) spp . ). ≪ / RTI >

The mixed fermented product of step 1) comprises i) mixing sawdust and a nutrient source; Ii) primary fermenting the mixture of step i) for 10 to 20 days; Iii) removing the gas; And iv) a secondary fermentation step.

The sawdust and the nutrient source may include all those which can be used as a feed for the flowering larva, and preferably include those which help form and grow the flowering larva.

The sawdust can be used both as a feedstock for the flowering larva and as a feed. Sawdust can be any kind of sawdust that can be inhabited by flowering larvae, but it can be preferably oak sawdust.

The nutrient source may include all the nutrients that can be used as a feed for the non-flowering larvae. Preferably, the nutrient source may be at least one nutrient selected from the group consisting of brown sugar, rice bran, soybean, and water.

The mixed fermented product of the sawdust and the nutrient source may be prepared by mixing 0.1 to 1.0% by weight of brown sugar, 1.0 to 5.0% by weight of rice bran, 1.0 to 5.0% by weight of soybean, and 30 to 40% . In one embodiment of the present invention, the mixed fermented product was fermented by mixing 60 kg of raw oats, 375 g of brown sugar, 1.5 kg of rice bran, 2 kg of soybean, and 20 L of water (Table 1).

The primary fermentation may be performed indoors, and may take 10 to 20 days, preferably 14 days. In one embodiment of the present invention, 7 days after the start of the first fermentation, the internal temperature was increased to 70 ° C, and the temperature gradually decreased, and it was confirmed that the temperature dropped to about 45 ° C after 14 days from the start of fermentation.

The step of removing the gas may take 1 to 10 days, preferably 3 days. In addition, the gas is degassed outdoors, which is the process of removing gas that is harmful to insect rearing during fermentation.

The secondary fermentation can be carried out under the same conditions as the primary fermentation and can be performed indoors. The secondary fermentation may take 10 to 20 days, preferably 14 days. The reason for the fermentation is that when the insect is fed with raw fish, it enters the high temperature state due to fungus or carbon dioxide emission, and it is fermented and the insect is damaged by the heat, so the gas is completely removed I will.

The step 2) comprises: i) sterilization by adding distilled water to brown rice; Ii ) To the sterilized brown rice, Paecilomyces 0.0 > tenuipes ) < / RTI > And iii) agitating the culture.

The step of sterilization by adding distilled water to the brown rice may be performed by preparing raw brown rice and adding distilled water of 30 to 50% by weight, preferably 40% by weight of the total weight of the raw brown rice to 100 to 140 ° C, preferably 115 Lt; RTI ID = 0.0 > 125 C < / RTI > The sterilization can be performed for 10 to 30 minutes, preferably for 20 minutes.

In the step of inoculating and cultivating the Chinese rice bran with the above-mentioned brown rice, the step of culturing the rice bran, tenuipes ) and culturing at 20 to 30 占 폚, preferably 25 占 폚 for 10 to 30 days, preferably 20 days.

The brown rice inoculated with the Cordyceps is in the range of 0.0001 to 10 parts by weight, preferably 0.0001 to 1 part by weight, preferably 0.0001 to 0.1 part by weight, preferably 0.0001 to 0.01 part by weight, based on 100 parts by weight of sterilized brown rice, , And most preferably 0.001 to 0.01 part by weight of the composition.

The step of agitating the cultured microorganism may be a step of agitating the microorganism by stirring at intervals of 2 to 3 days so that the cultured microorganism does not lump.

In the step 3), 1 to 10 parts by weight, preferably 5 parts by weight, based on 100 parts by weight of the mixed fermented product may be added, but the present invention is not limited thereto .

In one embodiment of the present invention, the method for preparing flowerless larvae containing cordyceps is as follows. We prepared 60kg of raw oats and 375g of brown sugar, 1.5kg of rice bran, 4kg of soybean and 20L of water to prepare nutritious sawdust. Put the nutritional sawdust in the bag and mix it evenly so that the nutrients are well mixed. The first fermentation takes 14 days and the internal temperature rises to 70 ℃ 7days after the start, and gradually falls to 45 ℃ after 14days. In this case, it is a process of removing gas for about 3 days outdoors, and it is a process of removing gas which is harmful to insect rearing when fermentation, and then re-fermented under the same conditions as the first fermentation. Thus, about 86 kg of a mixed fermentation product of sawdust and nutrient source was prepared. On the other hand, it was prepared as follows. Raw brown rice is prepared, and 40% by weight of distilled water of raw brown rice weight is added and sterilized at 121 캜 for 20 minutes. At that time, the brown rice was sterilized and absorbed distilled water to produce 4.3 kg of sterile brown rice in the form of rice. Paecilomycycles were purchased from the National Institute of Agricultural Science and Technology tenuipes ) J2 and incubated at 25 ° C for 20 days. 0.1 g of the Chinese cabbage fungus was prepared and inoculated. Then, the culture is stirred at intervals of 2 to 3 days so as not to lump to complete the culture. Thus, 4.3 kg of brown rice seedlings inoculated with Chinese cabbage was prepared. Then, the inoculated rice bran was inoculated into the mixed fermented product of the secondary fermented sawdust and the nutrient source, and the brown rice was mixed with 5 parts by weight of 100 parts by weight of the mixed fermented product of sawdust and nutrient (FIG. 1). Specifically, about 86 kg of the mixed fermented product was mixed with 4.3 kg of brown rice seedlings inoculated with Chinese cabbage. In this way, the feeds of the flowering larvae of the flowers containing Cordyceps were prepared.

The present invention can be used to produce a flowering larva having an increased number of individuals and an increased immunity by using a feedstuff of a flowering larvae containing a caterpillar fungus and a method for producing the same. In other words, it was possible to improve the productivity by promoting the growth and development of the larvae through the development of the feed containing the Cordyceps militaris which can strengthen the immunity so that the larvae can be raised in a healthy manner. Development The plant that consumed the caterpillar fodder containing the flower feeds the color of the medium, which is the digestive organ of the caterpillar larvae, is transparent and the activity is very high even in the sawdust which is transparent and the skin is clear, Respectively. On the other hand, when a larva is used as an edible insect, there is a step of shortening the larvae for several days in order to remove black streaks in the body. Since this procedure is omitted, weight loss due to fasting does not occur, This technology can maximize the number of individuals while feeding the larvae more healthily, which can contribute to the productivity improvement and the improvement of the farm household income of the domestic flower larvae.

FIG. 1 is a view showing a process for preparing a flower larva larva containing a Chinese cabbage fungus.
Fig. 2 is a graph showing weight gain tendency of flower larvae according to each feed.
Fig. 3 is a diagram showing the growth rate of the larvae in early 3 rd generation (at the 38th day of rearing) in the order of growth superiority.
Fig. 4 is a diagram showing the difference in skin color and growth of flowers larvae according to the nutrient source:
Left, control group; And
Uh, feed E.

Hereinafter, the present invention will be described in more detail in the following Examples. It should be noted, however, that the following examples are illustrative only and do not limit or limit the scope of the present invention. It will be understood by those of ordinary skill in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

< Example  1> Flower plain  Production of larval feed

Nutritious sawdust containing Chinese caterpillar fungus was prepared from the larvae of the flowers.

Add the nutrient source to raw fish and mix. 60kg of oatmeal prepared from raw oats, 375g of brown sugar, 1.5kg of rice bran, 4kg of soybeans and 20L of water were prepared. Put the nutritional sawdust in the bag and mix it evenly so that the nutrients are well mixed. The first fermentation takes 14 days and the internal temperature rises to 70 ℃ 7days after the start, and gradually falls to 45 ℃ after 14days. At this time, degassing is performed for about 3 days outdoors, and it is a process of removing gas which is harmful to insect rearing during fermentation, and then subjected to a second re-fermentation under the same conditions as the first. The reason for the fermentation is that when the insect is fed with raw fish, it enters the high temperature state due to fungus or carbon dioxide emission, and it is fermented and the insect is damaged by the heat, so the gas is completely removed I will. Thus, about 86 kg of a mixed fermentation product of sawdust and nutrient source was prepared.

On the other hand, it was prepared as follows. Raw brown rice is prepared, and 40% by weight of distilled water of raw brown rice weight is added and sterilized at 121 캜 for 20 minutes. At that time, the brown rice was sterilized and absorbed distilled water to produce 4.3 kg of sterile brown rice in the form of rice. Paecilomycycles were purchased from the National Institute of Agricultural Science and Technology tenuipes ) J2 and incubated at 25 ° C for 20 days. 0.1 g of Cordyceps bacillus was prepared and inoculated, and 0.002 part by weight of Cordyceps mellifera was inoculated against 100 parts by weight of sterilized brown rice. Then, the culture is stirred at intervals of 2 to 3 days so as not to lump to complete the culture. Thus, 4.3 kg of brown rice seedlings inoculated with Chinese cabbage was prepared.

Then, the inoculated rice bran was inoculated into the mixed fermented product of the secondary fermented sawdust and the nutrient source, and the brown rice was mixed with 5 parts by weight of 100 parts by weight of the mixed fermented product of sawdust and nutrient (FIG. 1). Specifically, about 86 kg of the mixed fermented product was mixed with 4.3 kg of brown rice seedlings inoculated with Chinese cabbage.

In this way, nutritional sawdust (feed E) containing caterpillar fungus, which is a feed of larva, was prepared.

< Example  2> Flower plain  Analysis of composition of larvae feed

<2-1> Preparation of feed

For the larval development, six feeds were used for the test. Six feeds were prepared as follows. First, the control group is oak sawdust sold in the market, and it is finely pulverized and used as feed for hatching insects. Fodder A and Fodder B are prototypes, commercialized products produced in domestic farms that raise blossom larvae. Feed C (Farm 1) was crushed and stored for a certain period of time, while Feed D (Farm 2) was mixed with 10% by weight of cow dung which was fermented in oak sawdust for a long time. Feed E was prepared using the nutritional sawdust containing the Chinese cabbage seeds prepared in Example 1 above. The ingredients and the number of fermentations used in the preparation of the six feeds are shown in Table 1.

division characteristic ingredient Fermentation
collection contrast No treatment 60 kg of raw oats, 20 liters of water - Feed A Prototype Oak rawpepper 60 kg, brown sugar 375 g, yeast 1.9 liters, lime 187 cc, rice bran 1.5 kg, soybean 4 kg, water 20 liters Episode 2 Feed B Prototype 60 kg of oak rawpot, 100 g of brown sugar, 1 kg of rice bran, 2 kg of soybean, 20 liters of water Episode 2 Feed C Farmhouse preparation Oak wood, water 1 time Feed D Farmhouse preparation Oak seeds, 10% bovine, water 1 time Feed E Containing Cordyceps 60 kg of oats, 375 g of brown sugar, 1.5 kg of rice bran, 4 kg of soybeans, 20 liters of water, Episode 2

<2-2> Analysis of composition of feed

The nutritional composition of each feed prepared in Example <2-1> was analyzed.

As a result, the dietary contents of diets were varied, and crude protein content tended to be higher for feed D and crude fat E for feed. Feed E showed the lowest value compared to the other experimental groups (Table 2).

In addition, vitamin A was significantly higher in feed D (farmhouse 2) than other farms, and other vitamins and E. coli were not detected. In particular, E. coli O157: H7 and Salmonella spp. Were common in six diets (Table 3).

Other heavy metals such as arsenic, cadmium, mercury and lead were mostly detected as negative or trace amounts (Table 4).

From these results, it can be seen that feed E (nutritional sawdust containing caterpillar fungus) is abundant in nutrients, and bacteria and heavy metals such as Escherichia coli and Salmonella can not be detected, and thus it is safe for flower larva.

sign division main ingredient moisture Crude protein Crude fat Crude fiber Views min Sulfur (S) One contrast 44.73 1.03 0.42 36.13 1.45 0.01 2 Feed A 37.52 2.85 0.23 40.56 1.64 0.03 3 Feed B 59.52 1.30 0.26 21.75 1.07 0.02 4 Feed C 79.66 1.80 0.10 6.90 3.48 0.03 5 Feed D 66.94 4.13 0.13 8.45 13.09 0.20 6 Feed E 54.85 2.44 0.53 17.20 0.93 0.03

division main ingredient Vitamin A (㎍ / 100g) Vitamin B1 ( mg / 100g) Vitamin B2 ( mg / 100g) Vitamin C ( mg / 100g) Total diet
fiber (%) E. coli O157 : H7 Salmonellaspp .(Qualitative) contrast 20.21 0.00 0.04 0.00 72.61 Non-detection Non-detection Feed A 27.42 0.00 0.01 0.00 79.37 Non-detection Non-detection Feed B 63.86 0.00 0.00 0.00 54.08 Non-detection Non-detection Feed D 165.32 0.00 0.00 0.00 20.18 Non-detection Non-detection Feed E 26.69 0.00 0.04 0.00 41.09 Non-detection Non-detection

* Feed C (farmyard preparation) can not collect samples due to larval mortality

sign division main ingredient arsenic( As ) cadmium( CD ) Mercury( Hg ) lead( Pb ) One contrast 0.00 0.00 0.10 0.00 2 Feed A 0.00 0.00 0.03 0.00 3 Feed B 0.00 0.00 0.05 0.00 4 Feed C 0.00 0.00 0.02 0.00 5 Feed D 0.00 0.00 0.08 0.00 6 Feed E 0.00 0.00 0.03 0.00

< Example  3> Flower plain  Effect of growth promoting effect of larvae feed

The six feeds prepared in Example <2-1> were fed to the larvae of the flowers and their weight gain was examined. Flower larvae are preserved in the Insect Industry and Environmental Insect Laboratory of the Department of Agricultural Biology, National Academy of Agricultural Sciences. White spider blooms ( Protaetia brevitarsis seulensis (Kolbe) larvae were purchased and used for experiments.

The developmental stage of flower ignorance is egg, larva, pupa, and adult stage. The larva stage is 1, 2, 3, and the developmental stage is 11, 19, 38 days A Study on the Growth Characteristics, Kim Cheol-hak, Jun-suk Lee, Min Soo Park, Gyu-Taek Park, Korean Society of Applied Entomology, 41 (1): 43-47 (2002)). In this study, larval stages were investigated during the development stage, and then the breeding period was stopped for protection of growth. The larval metamorphosis was affected by the microscopic changes such as physical contact and vibration, resulting in anomalies, metamorphosis, other physiological disorders, lethal diseases, etc. Therefore, direct contact was stopped and political rearing was carried out immediately before the pupa Table 5).

term 1 week 2 weeks 3 weeks 4 weeks 5 weeks 6 weeks 7 weeks 8 weeks 9 weeks Developmental stage 1st (11th) 2 nd (19th) Third (38th)

Table 6 shows the body weight of individual feeds after feeding, and FIG. 2 shows the growth rate among the individual feeds. According to the results of the study, the growth rate of the nutrient - free control group was 1.5 times higher than that of the non - nutritive control group after 6 weeks. The growth rate of feed A (prototype 1) and feed E And 5.8 times and 6.5 times, respectively. In the case of feed E, the growth at 7 weeks showed that the growth was equivalent to that of feed A (prototype 1), indicating that the feed E had a growth promoting effect. And improved results.

feed Research
Day 1 1 week 2 weeks 3 weeks 4 weeks 5 weeks 6 weeks 7 weeks 8 weeks 9 weeks contrast
(sawdust) 0.4 ± 0.21 0.54 + 0.18 0.57 0.21 0.44 0.27 0.59 0.25 0.50 + - 0.33 0.77 + - 0.38 0.68 ± 0.35 0.58 ± 0.54 0.59 ± 0.73 Feed A 0.40 0.27 0.95 + 0.22 1.23 + - 0.20 1.43 + - 0.33 1.83 + - 0.34 1.88 + - 0.34 2.22 0.30 2.21 ± 0.27 2.37 ± 0.18 2.33 ± 0.22 Feed B 0.397 + 0.33 0.527 0.20 0.556 + - 0.20 0.627 + 0.22 0.704 0.27 0.715 0.30 0.707 0.30 0.967 0.32 1.117 ± 0.33 0.84 0.36 Feed C 0.409 ± 0.29 0.678 + 0.24 0.83 0.26 0.93 + - 0.27 1.05 + 0.29 1.46 0.32 - - - - Feed D 0.397 + 0.18 0.969 + 0.41 1.068 ± 0.45 1.179 + - 0.41 1.414 + - 0.41 1.615 + - 0.34 1.753 + - 0.28 1.663 + - 0.31 1.823 + - 0.27 1.482 + 0.26 Feed E 0.407 0.21 1.235 + - 0.26 1.732 + - 0.22 1.986 ± 0.21 2.16 ± 0.19 2.209 ± 0.17 2.282 + 0.15 2.39 0.20 2.485 ± 0.20 2.61 ± 0.16

< Example  4> Flower plain  Larvae feed  Feeding  Comparison of post-development status

After 9 weeks of feeding, the survival rate of the larvae of each flower was examined as in Example 3 above. As a result, the survival rate was 20% in control, 90% in feed A and B, and 100% in feed D and E, respectively. Decreased populations during rearing are presumed to be the cause of homologous predation due to malnutrition or stress. The survival rate of larvae, larval skin color, larval odor, and major characteristics after 9 weeks of feeding were shown in Table 7.

Fig. 3 shows the growth rate of the larvae in early 3 rd generation (at day 38). Feed E, feed A, feed D, feed C, feed B, and control. Fig. 4 is a photograph comparing the skin color and the growth amount of the flower larvae according to the nutrient source. On the left is the flower larvae that feed the control feed and the right side is the flower larva that feeds the feed E. In FIG. 3 and FIG. 4, the skin color and growth of the flower larvae fed with feed E to the extent that they could be visually confirmed were excellent.

In order to evaluate the quality of each feed, scores were given as shown in Table 8. As a result, the feed E of 6 kinds of feeds was found to have the highest quality in terms of survival rate, skin color, larva odor, etc. (Table 9).

division survival
ratio(%) larva
Skin color Larva odor Main characteristics Decrease entity
cause contrast 20 Skin appears black Mushroom smell Allogeneic predation due to malnutrition
Growth decline phenomenon, difference in growth amount Malnutrition Feed A 90 Polish Fish meal odor Domestic universal commercial product, new irregular pus, homologous predation, epidermal gloss, built-in black poop Feed B 90 Matte Fish meal odor Sawdust is very similar to the growth pattern Feed C 0 Matte Smell of soil Developmental disruption, uneven development, diarrhea, anal dirt Malnutrition Feed D 100 Scabbard, matte Odor Growth rate Early deterioration Gradual deterioration, slowing of movement, Feed E 100 Insect epidermal gloss
Light yellow skin Mushroom incense,
Weak foul fish From 1 week after administration, it was remarkably good growth,

division Part Three Pus Standard deviation standard 5 (best 20, excellent 15, average 10, poor 5, very poor 1) weight The relative growth rate of the maximum growth rate test area and the minimum growth rate test area is divided into five stages Survival rate Application of relative standard to survival rate value in treatment area, 5 step classification Gloss, color Identify the skin, which is a larval health scale, and classify it into 5 levels (with or without gloss, skin color) smell Whether the smell of the larva to be used for food is good or not is classified into 5 stages

division contrast
(Raw fish) Feed A Feed B Feed C Feed D Feed E Pus 5 18 15 One 10 20 weight 5 18 10 One 15 20 Survival rate 5 18 10 One 15 20 Polish One 18 10 5 15 20 smell 10 15 10 5 One 20 Total score 26 87 55 13 56 100

Claims (9)

Mixed fermentation of sawdust and nutrient source; And brown rice inoculated with Paecilomyces tenuipes , as feedstuffs for raising larvae of white spotted moth larvae from day 1 to day 3,
The nutrient source is characterized by comprising brown sugar, rice bran, soybean and water,
Wherein the brown rice inoculated with the snow white caterpillar fungus is contained in an amount of 1 to 10 parts by weight based on 100 parts by weight of the mixed fermented product.
delete delete delete 1) preparing a mixed fermentation product of sawdust and nutrient source;
2) culturing the Chinese cabbage fungus; And
3) feeding the bacterium Enterobacteriaceae cultured in the mixed fermentation product of step 1) to the first to third instar larvae,
The nutrient source is characterized by comprising brown sugar, rice bran, soybean and water,
The step 2) comprises: i) adding distilled water to brown rice and sterilization at 100 to 130 ° C; Ii ) inoculating and culturing the sterilized brown rice with Paecilomyces tenuipes ; And iii) agitating the culture to produce a brown rice inoculated with the bacterium,
The step of feeding the cultured bacterium of step 3) is carried out by adding 1 to 10 parts by weight of the brown rice inoculated with the Chinese cabbage strain to 100 parts by weight of the mixed fermented product. Feed production method for rearing larvae.
6. The method of claim 5,
The mixed fermented product of step 1) comprises i) mixing sawdust and a nutrient source; Ii) primary fermenting the mixture of step i) for 10 to 20 days; Iii) removing the gas; And iv) a second fermentation step. The method for producing a feed for raising larvae of white spotted moth larvae of one to three days old. delete delete delete

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