KR100749227B1 - Method for preparing fermented microbial feed additives for livestock using mushroom media and fermented microbial feed for livestock using the same - Google Patents

Abstract

A method of manufacturing microbial feed supplements for livestock by inoculating aerobic and anaerobic strains into mushroom culture medium which are discarded and then culturing is provided to obtain microbial feed using the microbial feed supplements which has effects of enhancing immunity, the rate of weight gain and feed efficiency and removing odor from livestock facilities. Feed raw material with a moisture content of 25 to 78% is inoculated with 1 to 10L aerobic and anaerobic strains per ton of feed raw material and cultured. The feed material contains 50 to 70% by weight of mushroom culture medium, 1 to 5% by weight of mushroom by-products, 15 to 35% by weight of a moisture conditioning agent containing rice bran and simple feed, 5.5 to 15% by weight of corn flake, 3 to 10% by weight of pellets of palm peat, 2 to 8% by weight of pellets of wheat and barley, 2 to 8% by weight of hay and 1.5 to 6% by weight of trace components. The aerobic and anaerobic strains contain lactic acid bacteria, yeast fungus, Bacillus, photosynthetic bacteria and Actinomyces.

Description

Method for preparing fermented microbial feed additive for livestock using mushroom medium and fermented microbial feed for livestock using the same

1 is a flow chart showing the cultivation process of mycelium for the present invention

2 is a flow chart showing the manufacturing process of the present invention

The present invention relates to a high-quality fermented microbial feed fed to livestock and feed additives added to the microbial fermented feed, and more specifically, a medium containing a large amount of fiber despite the presence of a large number of nutrients, so that the medium is difficult to digest Is used for cultivating mushrooms so that it can be used as livestock feed, and microorganisms are isolated and cultured using the residues from which fiber support is decomposed. It relates to a method for producing fermented microbial feed additives for livestock using a mushroom medium maximized, and a fermented microbial feed for livestock using the same.

Mushroom is composed of moisture, carbohydrate, crude protein, crude fat, crude fiber, and ash, which not only has good protein and essential amino acid composition, but also has abundant trace elements, which plays an important role as a protein source. It is high.

Mushrooms have a good taste and aroma and are rich in various nutrients, and polysaccharides are known to be anti-cancer and immunomodulatory. They are used as health foods. Contains nutrients.

In particular, polysaccharides such as beta glucan (β-Glucan) contained in mushrooms and mushroom media are known to act to prevent the proliferation and metastasis of cancer cells by activating the immune function of cell tissues.

The medium used for mushroom cultivation contains a lot of nutrients but contains a large amount of fiber, which makes it difficult for livestock to digest.However, mushrooms are easily digested by absorbing and decomposing fiber during mushroom cultivation. Higher nutrients make it possible to effectively use nutrients such as vitamins and minerals.

In addition, the first place of livestock is a microbial depot, which contains a number of beneficial microorganisms and harmful microorganisms, and these microorganisms are responsible for decomposing livestock feed. Therefore, the role of microorganisms is absolute because the stabilization of fermentation in the first phase is the key to keeping the cells of beneficial microorganisms constant.

Therefore, by using the microbial agent cultured in the mushroom medium properly, it is possible to prepare a fermented microbial feed that can be useful for livestock by properly mixing the nutrient source and microorganism of the mushroom medium.

Conventionally, lactic acid bacteria, yeast, actinomycetes, Bacillus bacteria, photosynthetic bacteria and the like are cultured in a container together with a feed material containing mushroom medium as a microorganism. Among the above strains, microorganisms are cultured because the activity of lactic acid bacteria is the strongest. Yeast, actinomycetes, Bacillus bacteria, photosynthetic bacteria, etc. were killed in the process, and only lactic acid bacteria remained, so that the effect of the microbial preparation could not be obtained.

Nutrients demand for livestock will depend on factors such as retention, production, weight gain, lactation stage, breeding relationship, and season.In view of these fluctuations, different types of nutrients are needed to meet the needs of livestock. It is essential to use fermented microbial feed to improve feed availability and maximize useful microorganisms by mixing feeds, and to prevent the death of microorganisms in the process of culturing microorganisms to achieve the desired purpose of using fermented feed. .

In the present invention, microorganisms such as lactic acid bacteria, actinomycetes, yeasts, Bacillus bacteria, photosynthetic bacteria, etc., which have been proven effective in producing livestock feed, are cultured separately, or classified into aerobic and anaerobic fungi, and then discarded as organic waste. Inject the appropriate amount into the mushroom medium, and maximize the potency by increasing the digestion absorption by heat treatment and fermentation, and then directly used as fermented microbial feed or by mixing the general feed according to the purpose without loss of microbial preparation To provide fermented microbial feed and feed additives suitable for livestock raising.

In other words, the technical characteristics of producing fermented microbial feed and feed additives for livestock using a mushroom medium according to the present invention is to separate the screening and crushing by harvesting sanitary mushroom medium after harvesting bottle mushrooms, bag mushrooms, box cultivated mushrooms, etc. Then, in the preparation of fermented feed obtained by culturing microbial preparations such as yeast, lactic acid bacteria, Bacillus bacteria, photosynthetic bacteria, actinomycetes and mixing them with the general feed:

Culturing the lactic acid bacteria, yeast bacteria, Bacillus bacteria, photosynthetic bacteria, actinomycetes;

50 ~ 70% by weight of selected mushroom medium, 1 ~ 5% by weight of mushroom by-product, 15 ~ 35% by weight of moisture regulator made from rice bran and sweet rice, 5.5 ~ 15% by weight of corn flakes, 3 ~ 10% by weight of Pampit pellet, Adjusting the moisture of the feed material so that the water content of the mixed feed material of the barley is 2 to 8% by weight, 2 to 8% by weight of hay, and 1.5 to 6% by weight of the trace ingredient is 25 to 78%;

Lactic acid bacteria, lactic acid bacteria, yeast, Bacillus bacteria, photosynthetic bacteria, actinomycetes cultured in the liquid phase in the step using a inoculator injecting the selected liquid strain of 1 ~ 10ℓ per 1 ton of the feed material mixed with the moisture control agent to the mushroom medium in the step Obtaining a feed material inoculated with yeast, Bacillus bacteria, photosynthetic bacteria and actinomycetes, respectively;

Mixing the microbial strain evenly in each feed material inoculated with the microbial strain in this step;

The technical characteristics of the fermentation microbial feed that can be directly fed to the livestock by the step of culturing the liquid strains, respectively mixed in the feedstock in the step is characterized.

Another technical feature of producing fermented microbial feed and feed additives for livestock using mushroom medium according to the present invention is to separate and sort and shred by harvesting sanitary mushroom medium after harvesting bottle mushrooms, bag mushrooms, box cultivated mushrooms, etc. Next, in the preparation of fermented feed obtained by culturing microbial preparations such as yeast, lactic acid bacteria, Bacillus bacteria, photosynthetic bacteria and actinomycetes, and mixing them with the general feed:

Culturing the lactic acid bacteria, yeast bacteria, Bacillus bacteria, photosynthetic bacteria, actinomycetes;

50 ~ 70% by weight of selected mushroom medium, 1 ~ 5% by weight of mushroom by-product, 15 ~ 35% by weight of moisture regulator made from rice bran and sweet rice, 5.5 ~ 15% by weight of corn flakes, 3 ~ 10% by weight of Pampit pellet, Adjusting the moisture of the feed material so that the water content of the mixed feed material of the barley is 2 to 8% by weight, 2 to 8% by weight of hay, and 1.5 to 6% by weight of the trace ingredient is 25 to 78%;

For the feedstock obtained by mixing the moisture control agent in the mushroom medium in the step, the liquid strain cultured in the liquid state in the above step is divided into anaerobic fungi, which are lactic acid bacteria, yeast, Bacillus bacteria, photosynthetic bacteria, and aerobic fungi, which are actinomycetes. Injecting the anaerobic strain and aerobic strain 1 ~ 10ℓ per ton of each feedstock by using the inoculator to the feedstock to obtain two feedstocks inoculated with anaerobic strain and aerobic strain;

Mixing the microbial strain evenly in each feed material inoculated with the microbial strain in this step;

The technical feature is to obtain a fermented microbial feed that can be fed directly to the livestock by culturing the liquid strains mixed in the feedstock in the above step.

In addition, the present invention is fermented microbial feed obtained by the above-described manufacturing process hay, corn flakes, coconut shell pellets, cotton silk, wheat bark, corn stalks, molasses, limestone, salt, vitamins and minerals, such as a combination formulation to the target livestock Characterized in that it is used as a feed additive that can be arbitrarily mixed in a suitable ratio.

That is, 25 to 45% by weight of the fermented microbial feed obtained by the above manufacturing process as an additive, 10 to 30% by weight of hay, 5 to 15% by weight of corn flakes, 4 to 12% by weight of coconut shell pellets, 5 to 15 cotton husks. Weight%, cornstalk 5 ~ 14%, molasses 1 ~ 7%, limestone 0.5 ~ 2%, salt 0.5 ~ 2%, vitamins mineral combination 1 ~ 3% To obtain fermented microbial feed for livestock.

In addition, 25 to 45% by weight of the fermented microbial feed obtained by the above manufacturing process as an additive, 10-30% by weight of hay, 5-15% by weight of corn flakes, 4-12% by weight of coconut shell pellets, 5-15% by weight of wheat hulls. %, Cornstalk 5-14 wt%, molasses 1-7 wt%, limestone 0.5-2 wt%, salt 0.5-2 wt%, vitamin-mineral combination 1-3 wt% can be used for finishing To obtain fermented microbial feed for livestock.

The feeding method of the present invention is directly fed the fermented microbial feed obtained by the above-mentioned manufacturing method to livestock, or cultured in consideration of the type and growth time of livestock. It is divided into three periods, which are divided into middle and late stages.In case of milking, two periods are divided into growth stages and lactation stages, and one or two or more actinomycetes, lactic acid bacteria, yeast bacteria, Bacillus bacteria, and photosynthetic bacteria are selected. It can be fed into the mixer at a ratio and evenly mixed with additional feed.

The present invention for achieving the above object is to collect bottled mushrooms, bag mushrooms, or box cultivated mushrooms after harvesting sanitary mushroom medium degenerated;

The mushroom medium is sorted in a separator having a drum having a maximum particle size of 10 mm or less, and the mushroom medium and foreign substances not passed are separated and discharged by vibrators or by hand; Mushroom medium and fungi, including fungi that failed to pass through the sorting machine, are shredded to 6 mm or less and transferred to a mixer using a transfer conveyor equipped with an automatic crushing device;

As a moisture control agent for controlling the moisture of the mushroom medium to be transported by mixing the rice bran, hay, bark and mixed with the sweet rice feed to adjust the moisture of the feed material to contain 25 to 75% of the water;

At this time, when the raw material contamination, steam sterilization for 5 to 30 minutes at 120 ~ 125 ℃ in the moisture control mixing process before being introduced into the inoculation process;

For strain culture, anaerobic fungi such as yeast, lactic acid bacteria, Bacillus bacteria and photosynthetic bacteria are inoculated with the strain in a liquid medium and incubated for 2-5 days at 25-35 ° C. under anaerobic conditions, and aerobic strains such as actinomycetes are 115-126 ° C. Inoculated in sterilized liquid medium for 30-90 minutes at and incubated at 45-55 ° C. for about 14 days;

Lactobacillus, yeast, Bacillus, photosynthetic, and actinomycetes cultured in liquid state were inoculated by injecting 1 ~ 10ℓ / ton with the inoculator in the process of mixing the mushroom medium and the moisture control agent. Mix in a mixer so that the liquid strains are mixed evenly;

Actinomycetes use open containers for culturing liquid strains mixed in feedstock, and lactic acid bacteria, yeast, Bacillus bacteria and photosynthetic bacteria are stored in closed containers; Lactobacillus, yeast, Bacillus, photosynthetic bacteria are maintained at 2 ~ 40 ℃, actinomycetes are maintained at 30 ~ 70 ℃, sterilization apparatus that can sterilize the interior of the culture chamber for 6-8 hours at a temperature of 65 ~ 80 ℃ Culturing the microorganisms in the feed material in a culture chamber equipped with;

In order to improve the nutrition of feed ingredients obtained by culturing microorganisms, feed additives used as fermented microbial feeds by adding a certain amount of additives such as sweet rice and hay to each target feed in the process of transporting feed materials cultured with microorganisms. Getting;

The obtained fermented microbial feed additive is mixed with a single strain or a complex strain and cultured in consideration of the type and growth time of the livestock. In the case of the beef cattle, it is divided into three periods, which are classified as growing stage, growing stage, and late stage. In the case of milking, microbial feed additives containing actinomycetes, lactic acid bacteria, yeasts, Bacillus bacteria, and photosynthetic bacteria are divided into two phases, which are divided into growth periods and milking periods. To mix; And packaging the fermented microbial feed according to animal characteristics for each strain.

The combined ratio of the microorganisms of fermented microorganisms is 25-30%, actinomycetes 25-30%, yeast 15-20%, Bacillus 15-20%, and other fungi up to 20% with respect to 100% of the combined microorganisms.

The medium used for mushroom cultivation contains many nutrients, but it contains a large amount of fiber, which makes it difficult for livestock to digest.However, mushrooms are absorbed and decomposed in the process of growing mushrooms, resulting in higher plasticizing nutrients in the mushroom by-products. Can improve the utilization of nutrients, such as minerals.

Lactic acid bacteria produce lactic acid during the culturing process to lower acidity (pH4) and to produce hydrogen peroxide (H ₂ O ₂ ) to inhibit the growth of pathogenic microorganisms and prevent soil diseases.

In addition, by producing various bioactive substances (vitamins, amino acids, nucleic acids, etc.), antibacterial substances, and anticancer substances, crops increase plant self-defense ability, and in the case of livestock, it shows stabilization of intestinal microflora, increased feed efficiency, and increased disease resistance. .

The strain includes Lactobacillus acidophilus, a bacterium, and Streptococcus lactis, a bacterium.

Yeast is a major microorganism of heat generation during fermentation of farm by-products such as rice bran and wheat bran, and has excellent ability to decompose organic matter, and has excellent fermentation and viability under any adverse conditions.

Therefore, when it is included in the feed and sprayed on the barn, it is excellent in removing odor. In addition, amino acids, vitamins and other ingredients necessary for the growth of crops and livestock to increase the palatability of the feed, mainly used strain is Saccharomyces cerevisiae (saccharomyces cerevisiae).

Actinomycetes are natural organisms of pathogenic fungi that grow in soil and produce beneficial substances that kill antibiotics or stop growth.

High quality activated soils or soils with high organic content are characterized by high density of actinomycetes. The ability to inhibit the disease is due to the large amount of antibiotics produced by actinomycetes.

The unique smell of the soil is also the smell of actinomycetes. The reason for the post fermentation of mushroom medium after sterilization is also to cultivate actinomycetes, and it is well known that the culture of the actinomycetes is well known that there is little contamination of various germs in the process of inoculation after mushroom spawn inoculation. It is a genus of Streptomyces.

Photosynthetic bacteria are bacteria that perform photosynthesis using sunlight like plants. It is crimson and has a very high protein content in the cells and produces a large amount of various vitamins, minerals, bioactive substances and antibacterial and antiviral substances.

Photosynthetic bacteria grows while ingesting various odor generating substances such as ammonia, hydrogen sulfide, and organic acids, and thus are excellent in removing odors. Photosynthetic bacteria are also microorganisms that can prevent the gas generation damage caused by immature organic matter in the barn by performing a gas removal role.

It is also used as a microorganism for water purification in fish farms because of its excellent ability to purify contaminated dirty water. Strains mainly used in agriculture are classified as yellow non-sulfur bacteria as genus Rhodopseudomonas. It promotes rooting effect and flower bud formation, and is effectively used to promote pigmentation.

Bacillus subtillis and the like improve the absorption of feed by converting high molecular weight organic matter into low molecules.

According to the present invention, useful fungi (saccharomyces cerevisinc), such as yeast, supply essential amino acids, vitamins, trace elements and various bioactive substances produced in cells, which can lead to a qualitative improvement in productivity and quality, and intestinal tracts. In addition to stimulating metabolism, the cell culture continued after manure was excreted to reduce ammonia gas and volatile fatty acids, which are the main causes of animal manure odor.

The metabolism of the microorganisms promotes digestion by activating digestion, and many enzyme-producing strains also synthesize nutrients, such as essential amino acids, required for increased flow by producing and activating digestive enzymes that enhance fermentation of nutrients. Improve the utilization of feed, such as decomposition.

It contains dozens of strains with antibacterial activity, including streptomyces, which have a strong effect on pathogens and are also effective against latent mastitis.

Lactobacillus promotes the removal of pathogenic microorganisms and cholesterol.

Photosynthetic bacteria are extremely anaerobic bacteria that activate beneficial bacteria in the intestines of livestock.

Carotenoids in maize, etc. increase the disease resistance by eliminating toxic oxygen that promotes aging and lowering immune system activity during the metabolism of cells and enhancing the activity of T-cells that fight against pathogenic microorganisms. To prevent disease, to protect the animal's oviduct and prevent aging to increase the breeding efficiency.

Bacillus sp. Microorganisms isolated from traditional fermented foods promote the activity of hydrolase amylase, protease, etc., and greatly enhance fermentation and degrading ability to prevent diarrhea and stool.

In addition, the palatability is increased by the fermentation odor increases the feed intake.

The fermented feed using the mushroom medium of the present invention is based on the fiber raw material in which the mushroom colonies are cultured, and excellent functional feed obtained by fermenting fungi such as lactic acid bacteria, actinomycetes, yeast bacteria, Bacillus bacteria, photosynthetic bacteria and the like beneficial to livestock. to be.

Therefore, according to the present invention, by mixing the feed material and additives based on the fiber material in which the mushroom cells are cultured, fermented into fungus that is beneficial to livestock, and feeds it by the appropriate combination of excellent nutrient source and fermentation bacteria of mushrooms and heat treatment process in manufacturing It is possible to provide a high quality functional feed that can take full advantage of the probiotics.

Looking at the step of producing the fermentation microbial feed and feed additive according to the present invention in detail for each step as follows.

1 is a flow chart showing the cultivation process of mycelium for the present invention.

The cultivation process of the mycelium to obtain the mushroom medium as a raw material for producing the fermentation microbial feed and feed additive of the present invention according to the flowchart is as follows.

Stage 1 (Preparation of Cultivation Facility, S10)

Facilities required for mushroom bottle cultivation include a laboratory that can mix laboratory and sawdust materials, a sterilization and cooling room for sterilization, an inoculation room for inoculating strains, a culture room for cultivating mycelia, and aging strains after cultivation. It consists of a germ scraping room that can be made, a footing room that can produce fruiting bodies, a suppression room to improve quality, and a cultivation room that can produce mushrooms.

Stage 2 (Machinery and Instrument Preparation, S11)

A sawdust sieve for evenly sawdust, a mixer to control moisture, a bottle to hold sawdust material, a sterilizer with a boiler and sterilizer to sterilize the bottle, sterilization workbench, inoculator In addition, there must be a deaerator to remove the waste sawdust from the container after it has been scrapped and grown.

Step 3 (Preparation of Materials and Additives, S12)

The main ingredients are corn cob, sugar cane watermelon, cotton silp, cotton silt and rice bran. The types of sawdust with the maximum mixing amount not exceeding 10% are poplar, oak, and pomegranate. The materials are also different.

In addition, sawdust treatment is better to use less resin after 3-6 months left than raw sawdust. If the particles of sawdust are too thin, less than 1mm, mycelial growth is poor and quantity is low due to lack of air.

The additive adds agricultural by-products or industrial by-products such as rice bran, wheat bran, corn cob, etc. to increase the growth of the bacteria.The water content is 63 ~ to 100% by volume evenly mixed. Adjust to 68%.

4th stage (hospitalization work, S13)

Moisture-controlled sawdust broth is placed in a 850cc plastic bottle with a bottler, and squeezed up to 2cm from the top of the bottle, and the surface is flattened. Drill a hole, remove the sawdust from the bottle opening, cover the cap and sterilize. This process can be done consistently with automatic feeders and stopper machines.

5th stage (sterilization work, S14)

Sterilize at 105 ℃ (about 1.0kg / ㎠) in autoclave for 4 ~ 6 hours. During sterilization, steam should always enter and exhaust. Sterilization time is high when sterilization time is short. After sterilization, transfer to the cooling chamber and cool the medium.

6th step (inoculation and incubation, S15)

After cooling the medium in the cooling room, transfer to the inoculation room for inoculation. Inoculation is inoculated 5 ~ 8g in the sterile chamber, sterilization of the germicidal room after sterilization of the room with ultraviolet light sterilization 3 ~ 5% or sulfur fumigation, etc. to prevent the contamination of various germs.

The mycelial growth temperature of mushrooms is in the range of 5 ~ 35 ℃ but the optimal temperature for mycelial growth is 25 ~ 28 ℃.

However, the room temperature of the incubation chamber takes into account the respiration heat inside the bottle and performs military cultivation at 16-20 ° C. to reduce the incidence of various germs. When the temperature of the culture chamber is lower than 5 ° C or higher than 30 ° C, the bacteria may stop or die.

7th step (Sorting and scraping bacteria, S16)

Inoculate the bacteria and incubate the bacteria in the culture room for 20 to 25 days. However, until the mycelial culture is completed about 90% of the culture, the bacteria are often selected and removed. The bacteria scraper removes the bottle cap from the alcohol-stained bacteria scraper and scrapes the inoculum.

8th Stage (Footing Organics, S17)

When the germicidal scraping bottle is transferred to the foot room and the room temperature is maintained at 12-15 ° C. and the humidity at 90-95%, new mycelia bloom from the inside of the bottle after 5-8 days, forming yellow and light yellow droplets. After 4 to 5 days, young mushrooms develop, grow about 0.5 seconds in the bottle, and transfer to the restraint room when the small shape of the cap is formed.

When organic fertilization is carried out, the luminosity of the grower should be adjusted to about 5 ~ 100 lux. If the lux is more than 10 lux depending on the strain, there are some mushrooms with poor quality due to the change of the shade and stem.

The period of suppression of fruiting body is about 7 ~ 10, the temperature of the suppression room is kept at 3 ~ 4 ℃, the humidity is 80 ~ 85%, and the ventilation is made 2-3 times daily to make the mushroom shade and stand strong.

9th Stage (Growth and Harvest, S18)

When moving from the restraint room to the growth room, a 12 ~ 13cm paper shade should be put around the entrance of the bottle. The growth room humidity is 75 ~ 80%, and the temperature is adjusted to 6 ~ 8 ℃ while illuminating the brightness by 50 ~ 100lux. However, if temperature, ventilation and humidity are not properly controlled, the growth of fruiting bodies may be irregular or delayed. The harvest time varies depending on the strain, but mature fruiting bodies are formed in 7-10 days in the growth room. At this time, remove the paper shade and harvest the fruiting body.

Stage 10 (Dehydration and Harvesting, S19)

Waste media are collected from the bottles, papers or boxes from which the fruiting bodies are harvested to obtain waste media for use in the present invention.

Figure 2 is a flow chart showing a process for producing a fermented microbial feed of the present invention using the mushroom medium obtained by the above-described manufacturing process.

Referring to the process of manufacturing the fermentation microbial feed and feed additive of the present invention by the flow chart as follows.

Stage 1 (Medium Collection Process, S20)

Harvest bottle mushrooms, bag mushrooms, or box cultivated mushrooms, and then collect hygienic mushroom media.

2nd stage (selection and foreign substance removal process, S21)

The mushroom medium is sorted in a sorter equipped with a 10 mm drum with a maximum passing particle size, and the mushroom medium and mushroom by-products, fungi and foreign substances that have not passed through are separated and discharged by vibrators or by hand.

3rd step (crushing process, S22)

Mushroom medium, fungi, mushrooms, etc., which have not passed through the sorting machine, are shredded to 6mm or less and transferred to a conveying conveyor equipped with an automatic crushing device. At this time, the mixing ratio of the mushroom medium, fungus, mushrooms is not limited, and usually mushrooms are mixed within 100% by volume of the total feed material to within 10% by volume.

4th stage (moisture regulator input process, S23)

To meet the conditions under which fermentation microorganisms can be cultivated in an optimal environment, the rice bran, hay, and bark are mixed in a reel, auger, or ribbon type mixer as a moisture control agent in a mushroom medium, and sweet rice feed is added thereto. Adjust the moisture content of water, which has a significant effect on the culture of bacteria according to feed characteristics.

Since the ratio of mixing rice bran, hay, and bark used to control moisture is not necessarily limited, the mixing amount of these moisture regulators is increased or decreased by the moisture of the mushroom medium at a uniformly distributed mixing ratio. Is determined.

The condition that satisfies the optimum moisture content is that the composition of the mushroom medium to contain 20 to 65% moisture when 55 to 60% moisture.

Hay, together with moisture control, forms a space to supply oxygen for fermentation microorganisms.

The bark of the bark and cotton bark and palm bark may be used.

Mushroom medium and moisture control agents are mixed and inoculated with the strain required.

5th step (preparation of fermentation microbial strain, S24)

Bacterial fermentation microorganisms are lactic acid bacteria, yeast, Bacillus bacteria, photosynthetic bacteria, fungal fungi using actinomycetes are fermented and propagated according to the conditions for each strain in each fermenter.

In the strain production process, 1 g of water is uniformly mixed with 20 g of a grain powder such as wheat flour, sterilized at 115-126 ° C. for 30-90 minutes, cooled to 20 ° C., and seeded in a liquid medium on a clean bench. Then incubated in the culture chamber.

The proper culture conditions for each strain were aerobic bacteria for 14 days at 45-55 ℃ for 14 days in a culture vessel equipped with an aeration device with oxygen supply, and anaerobic bacteria for 2-5 days at 25-35 ℃ in a sealed container. do.

6th step (inoculation process, S25)

Inoculate the lactic acid bacteria, yeast bacteria, Bacillus bacteria, photosynthetic bacteria, actinomycetes 1 to 10ℓ / ton by using an inoculator in the mushroom medium mixed with a moisture control agent in a mixer.

At this time, the inoculation of the strain can be inoculated with only one single strain, or divided into two groups of monarchs separated into anaerobic strains and aerobic strains. Sterilize at 120 ℃ for 5 ~ 30 minutes with steam sterilizer.

7th Step (Mixing Process, S26)

Mix the moisture-controlled feedstock with the density of the sprayed liquid strains to be even.

8th Step (Container Storage Process, S27)

Inoculation to feedstock To feed mixed liquid strains, feedstock is stored in a container made of a synthetic resin such as a plastic pack, a closed tone bag, or a metal such as ordinary steel, stainless steel or aluminum.

At this time, it is stored using 5 ~ 2000ℓ containers such as open plastic box or open metal container.Aerobic fungi that need oxygen like actinomycetes use open containers that can supply oxygen, lactic acid bacteria, yeast bacteria, Bacillus bacteria, photosynthetic bacteria. Anaerobic fungi that do not require oxygen, such as, use a closed container to block the oxygen supply.

9th step (cultivation process, S28)

Lactobacillus, yeast, Bacillus, and photosynthetic bacteria are maintained at 2 to 40 ° C, actinomycetes are maintained at 30 to 70 ° C, and cultured to actinomycetes in order to maintain the conditions of the culture room suitable for the strain. Oxygen supply device to inject external air and exhaust internal air to supply oxygen to the inside, an air circulation device to reduce the temperature deviation in the culture chamber and the heat loss in the actinomycetes culture chamber, and 65 ~ 80 ℃ inside the culture chamber if necessary Incubate the fermentation microorganisms in the feed material in a culture chamber equipped with a sterilization apparatus capable of sterilization at a temperature of 6 to 8 hours.

This culture provides a fermented microbial feed additive that can be used directly as a fermented microbial feed or mixed in a general feed. The fermented microbial feed and fermented microbial feed additive are lactobacillus, yeast, bacillus and photosynthetic bacteria. As two groups of feeds and additives that are cultured or classified into aerobic and anaerobic strains, each strain can be used in combination with a compounded feed in a fixed ratio according to the characteristics of the livestock.

10th Step (Synthesis Feed Process, S29)

In order to improve the nutrition of the feed, fermented feed additives, which are fermented microorganisms, are fed to the blender, and a certain amount of additives such as sweet rice and hay are added to each target feed. The fermentation microorganisms cultured uniformly are optionally added in proportion to each strain.

11th Step (Combination Process, S30)

Considering the type and growth time of the livestock, the fungal feed is selected from actinomycetes, lactic acid bacteria, yeast bacteria, Bacillus bacteria, and photosynthetic bacteria, and mixed into an additional feed at an arbitrary ratio and mixed uniformly with additional feed.

In the case of beef cattle, it is divided into three stages, which are classified as growing stage, growing stage, and late stage of breeding. In the case of milking, it is divided into two stages which are classified as growing stage and lactation. As the feed content of protein, fat, fiber, and ash varies according to its characteristics, it is appropriately selected and mixed in consideration of nutritional balance and economic aspects.

12th Step (Packaging Process, S31)

In accordance with livestock breeding conditions and logistics characteristics, 20-30 kg of small packages, other large packages, and ton bag packaging should be packaged according to livestock farms.

13th Step (Secondary Culture Process, S32)

The fermented microbial feed packaged according to the characteristics of the livestock is gradually secondary cultured.

Fermented microbial feed of the present invention prepared by the above process is used as a feed additive to be directly fed or mixed with the general feed according to the condition of the livestock.

The salary is determined in consideration of the type of animal, the growth period, and the growing condition.For the formulation of fermented microorganisms, 35% by weight of fermented microbial feed (feed additive) is optimally mixed. Milking is about 30kg per day on the basis of 28kg of milk production per day.

The titers of the microorganisms used in the present invention are shown in Table 1 below.

Total cell count Actinomycetes leaven Lactobacillus Etc 107 ~ 109cfu 103 ~ 109cfu 107 ~ 109fu 107 ~ 109cfu 107 ~ 109cfu

The results of analyzing the components of the fermented microbial feed of the present invention are shown in Table 2 below.

Component Analysis (%) moisture Crude protein Crude fiber View minutes Other (crude fat, minerals) NDF ADF 35-60 4.5-10 14-16 1.8 ~ 6 20.7 ~ 33 25 17.5

Trace elements include calcium, phosphorus, sodium, vitamins and minerals.

The fermented microorganism is a product cultured for each strain by using a mushroom medium, fermented fiber refers to a fermented microbial feed that is mixed and packaged with a general feed according to the characteristics of the animal when the product is shipped.

In order to test the feed efficacy of the fermented microbial feed and the feed additive of the present invention, the fermented microbial feed prepared using the bottle mushroom medium was fed to 100 beef heads and various good results were obtained.

The fermentation agent (lactic acid bacteria + yeast) produced by Medi-Pal is used, and there is little smell of fly and manure. Normally, it took about 350,000 won to buy flies each year to get rid of flies, but it cost about 10,000 won to buy flies.

In addition, since the floor is dry and does not smell, the cost of litter is greatly reduced, and labor costs such as floor cleaning costs are also reduced, enabling eco-friendly livestock farming.

The use of antibiotics has decreased considerably since the mushroom medium was grown from fermented microbial feed.

The rearing of cattle with fermented microbial feed significantly reduced the respiratory diseases and significantly increased immunity.

An example of breeding cattle is shown in Table 3 below.

(Unit: KG) Fermented Microbial Feed Wheat Mace corn Short vein vitamin Incal Limestone Salt sum 2100 600 600 600 10 50 40 8 4000

An example of rearing cattle before the rearing is shown in Table 4 below.

(Unit: KG) Fermented Microbial Feed Wheat Mace corn Short vein vitamin Incal Limestone Lupines sum 900 40 300 50 3 5 2 200 1500

An example of the rearing of cattle after fertilization is shown in Table 5 below.

(Unit: KG) Fermented Microbial Feed Wheat Mace corn Short vein Salt Incal Limestone Lupines sum 350 25 350 25 1.5 0 0 250 1000

The results of the shipment grade of cows fed compound feed are shown in Table 6 below.

The number of days weight Breeding days Back fat Fillet area Conductor weight BMS Meat color Local color Organization Final rating Start Shipment month mℓ ㎡ Kg NO NO NO NO 1.11 11 heads 205 675 23 10 85 400 5 4 3 One B1 135 529 19 5 72 290 3 4 3 2 A2 135 653 23 20 74 395 6 4 3 One C1 + 130 657 23 17 82 388 4 4 3 One C1 216 729 23 16 85 451 6 4 3 One C1 + 165 605 23 12 69 361 6 4 3 One B1 + 165 623 23 12 69 356 4 4 3 One B1 135 693 23 11 110 422 7 4 3 One A1 + 170 679 23 11 102 418 5 4 3 One A1 157 679 23 14 83 417 5 4 3 One B1 158 593 23 8 87 342 5 5 3 One A1 4.20 9 heads 164 760 22 13 105 444 6 5 3 One B1 + 143 660 24 18 77 378 3 5 3 2 C2 140 653 24 16 77 383 4 5 3 One C1 136 674 22 8 89 374 5 5 3 One A1 162 651 24 13 82 377 7 5 3 One B1 + 153 654 22 10 83 379 7 5 3 One B1 + 146 646 24 14 87 360 3 5 3 2 B2 145 673 24 10 92 379 3 5 3 2 B2 446 658 24 14 77 364 6 5 3 One B1 + system 3,406 13,144 459 252 1,687 7,678 100 90 60 24

The results of the shipment grade of cows fed fermented microbial feed are shown in Table 7 below.

The number of days weight Breeding days Back fat Fillet area Conductor weight BMS Meat color Local color Organization Final rating Start Shipment month mℓ ㎡ Kg NO NO NO NO 9.8 4 heads 140 670 23 10 91 421 One 5 3 2 B3 140 624 23 9 86 407 6 4 3 One B1 + 140 708 23 9 92 447 6 4 3 One B1 + 140 600 23 3 88 368 4 5 3 One A1 9.9 6 heads 160 644 19 7 84 398 7 5 3 One A1 + 160 626 19 11 79 389 5 4 3 One B1 150 672 23 14 84 434 6 4 3 One B1 + 150 670 19 14 84 437 6 4 3 One B1 + 150 670 23 30 80 440 6 4 3 One C1 + 140 736 23 7 90 459 4 4 3 One A1 8.27 1 head 150 641 23 7 87 386 7 5 3 One A1 + 10.12 Two Heads 120 700 24 15 86 449 6 4 3 One C1 + 130 710 24 9 95 451 4 4 3 One A1 10.14 Two Heads 135 761 24 13 100 457 7 5 3 One B1 + 120 634 24 6 110 385 7 5 3 One A1 + 10.27 Two Heads 160 676 20 9 86 386 4 5 3 One B1 145 765 20 8 88 415 5 5 3 One A1 11.11 1 head 150 610 21 6 91 357 7 4 3 One A1 + 12.4 6 heads 135 733 22 12 102 445 7.1 5 3 1.2 1 + A 130 732 22 16 99 444 5.1 5 3 1.3 1C 140 605 23 12 85 367 8.1 5 3 1.2 1 ++ A 145 817 22 15 93 496 6.3 5 3 1.2 1 + B 143 693 23 9 85 420 2.2 5 3 2.1 2A 140 580 25 8 83 352 5.1 5 3 1.3 1A

The results of the shipment grade of the cows fed the compound feeds are shown in Table 8 below.

The number of days weight Breeding days Back fat Fillet area Conductor weight BMS Meat color Local color Organization Final rating earmark Shipment month m kg NO NO NO 1.10 6 heads 333 546 22 13 83 321 8 5 3 One 1 ++ B 323 670 23 8 92 395 8 5 3 One 1 ++ A 334 576 22 6 79 328 8 5 3 One 1 ++ A 321 685 23 18 85 379 3 5 3 One 2B 324 668 23 9 76 403 8 5 3 One 1 ++ B 3215 664 23 18 78 394 4 5 3 One 1C 1.18 325 680 23 8 78 392 8 5 3 One 1 ++ B 1.26 6 heads 222 * 23 8 85 429 6 5 3 One 1 + B 328 * 23 11 86 365 9 5 3 One 1 ++ B 341 * 23 9 96 352 7 5 3 One 1 + A 326 Average 23 8 99 383 9 5 3 One 1 ++ A 2103 674 23 9 111 397 7 5 3 One 1 + A 2. 23 10 heads 326 * 697 24 11 90 408 3 5 3 One 2A 3223 726 24 13 88 420 6 5 3 One 1 + B 335 711 24 21 76 416 8 5 3 One 1 ++ C 3217 839 24 23 85 506 4 5 3 One 1C 3218 740 24 16 74 420 5 4 3 One 1C 3221 830 24 12 82 497 8 5 3 One 1 ++ B 3220 662 24 12 89 392 8 4 3 One 1 ++ B 3219 747 24 18 75 446 7 4 3 One 1 + C 329 763 24 10 108 465 9 4 3 One 1 ++ A 328 624 24 6 83 376 8 5 3 One 1 ++ A

The results of the comparative analysis of the compound feed and the fermented microbial feed are shown in Table 9 below.

division Compound Feed Fermented Microbial Feed Six Grade B Award 78% Grade C 22% Grade B or higher 80.5% Grade C 19.5% Meat quality Level 1 Award 73% Level 2 Award 27% Grade 1 or higher 91.4% Grade 2 or lower 8.6% Live weight 685 kg 683 kg Conductor weight 398 kg 410 kg Conductor rate 58.1% 60.1% Feed cost 30% savings Environment Occurrence of various diseases, odors and pests in the barn Disease free, pleasant growth reduces treatment costs and eliminates odors, resulting in fewer pests

The results from the use of mushroom media as feeds were quite encouraging.

41 cattle were fed and fed with a general formula, 78% of grade B and above, 22% of grade C, and 73% of grade C and 27%, respectively.

46 cows were bred and shipped as fermented microbial feed of the present invention. In terms of meat, grade B or higher is 80.5%, grade C 19.5%, and grade 1 or higher is 91.4% and grade 2 or less is 8.6%.

Comparing these two groups, cattle raised with fermented microbial feeds had a B grade of 2.55 higher than those fed compound feed, and 18.45 higher than meat grade 1 or higher.

Upgrading grades in cattle breeding farms requires considerable effort, which is a dramatic result.

Monthly average test results are shown in Table 10.

Black month Gyeongsan-woo Milking cow Delivery head Acetic acid delivery flux Retention rate Protein Solidity Somatic cells Correction head Pregnancy head Days left Dry days Average 500 thousand or more 0501 39 31 2 10 36.4 3.5 3.32 8.93 94 One 7 0 164 73 0502 39 32 One 10 35.4 4.29 3.33 8.86 131 3 0 0 168 71 0503 39 0 0 10 35.4 0 0 0 0 0 0 0 168 71 0504 39 26 7 8 38.7 3.82 3.44 9.09 192 2 0 0 170 79 0505 39 22 One 8 37.5 3.93 3.3 8.78 144 One 0 0 170 80 0506 39 0 0 8 37.5 0 0 0 0 0 0 0 170 80 0507 33 29 7 11 32.3 4.34 3.01 8.47 148 3 0 0 159 82 0508 36 26 3 10 31.1 3.87 3.17 8.7 291 5 0 0 159 82 0509 34 27 5 10 33.6 3.72 3.14 8.71 148 One 0 0 182 81 0510 34 0 0 10 33.6 0 0 0 0 0 0 0 182 81 0511 37 31 10 12 34.4 3.95 3.34 8.94 267 4 0 0 250 87 0512 37 0 0 12 34.4 0 0 0 0 0 0 0 250 87 0601 37 29 3 12 42.6 3.82 3.4 8.82 156 2 0 0 375 84

January individual test results are shown in Table 11 and Table 12.

Abbreviated name Mountain car Milking days Achievement 305 Daily Maturity Last breed flux Somatic cells Retention rate Protein Solidity MUN flux Maintenance flux Maintenance Modified date Cum code 34 4 367 25.1 57 3.96 3.48 8.91 no 15881 492 15371 477 none none 35-1 2 273 44.3 157 3.61 3.27 8.64 no 14180 541 14281 561 none none 38 2 246 0 0 0 0 0 no 12965 453 12701 455 none none 40 One 308 38 65 3.64 3.31 8.84 no 12822 538 14204 614 none none 24 3 386 0 0 0 0 0 no 12479 444 13103 465 20041103 H-292 31 One 154 44 11 3.88 3.09 8.74 no 12433 439 13444 477 none none 27 5 289 46.4 155 3.08 2.94 8.1 no 12234 441 11879 434 none none 18-1 4 291 32.8 140 4.06 3.8 9 no 11948 513 11530 503 none none 45 2 1115 40.9 112 3.84 3.44 8.84 no 11540 413 11466 414 20041228 H-292 30 2 877 0 0 0 0 0 no 11376 375 12257 409 20040526 H-288 49 4 155 46.3 390 3.91 3.26 8.63 no 11072 451 11834 481 none none

Abbreviated name Mountain car Milking days Achievement 305 Daily Maturity Last breed flux Somatic cells Retention rate Protein Solidity MUN flux Maintenance flux Maintenance Modified Date Cum code 21 One 284 44.4 91 4.04 3.78 9.03 no 10948 437 11827 488 none none 66 One 383 0 0 0 0 0 no 10925 431 11147 443 none none 16 2 118 46.4 140 3.74 3.59 8.86 no 10625 396 11098 412 none none 22-1 One 384 32 387 3.58 3.13 9.15 no 10126 404 10717 437 none none 6 3 119 57.8 63 3.85 3.28 8.85 no 9965 379 10392 393 none none 19 One 253 32.8 143 3.93 3.46 9 no 9422 413 10259 462 none none 17-1 2 410 56.2 11 3.85 3.24 8.82 no 9287 374 9502 386 none none 12 2 265 29.9 74 4.17 3.8 9.11 no 9164 343 9059 347 none none 48 6 263 0 0 0 0 0 no 8673 370 8697 372 none none 43 One 186 45.3 8 4.25 3.77 9.25 no 8652 332 9693 377 none none 29 One 299 0 0 0 0 0 no 8511 326 9036 355 none none 5 6 141 37.4 18 3.83 3.59 8.74 no 8198 261 9019 303 none none 15 One 139 0 0 0 0 0 no 8673 370 8697 372 none none 4 5 14 47.3 21 3.71 3.29 8.77 no 0 0 0 0 none none 20 4 12 60.5 100 3.19 2.85 8.26 no 0 0 0 0 none none 2 3 64 47 1082 3.32 3.18 8.25 no 0 0 0 0 none none 25-1 3 60 45 73 3.82 3.28 8.85 no 0 0 0 0 none none 46 3 60 44.8 682 3.85 3.19 8.85 no 0 0 0 0 none none 42 3 805 0 0 0 0 0 no 0 0 0 0 none none 47-1 3 64 44.3 73 3.56 3.03 8.59 no 0 0 0 0 none none 10-1 2 59 47.4 85 4.03 3.6 9 no 0 0 0 0 none none 50 2 63 36 111 3.85 3.64 8.87 no 0 0 0 0 none none 67 2 11 40 67 4.17 3.81 9.1 no 0 0 0 0 none none 11-2 One 73 39.8 69 4.25 3.86 9.19 no 0 0 0 0 none none 44 One 70 39.8 67 4.08 3.32 9 no 0 0 0 0 none none 36 One 63 43 80 4.18 4.04 9.26 no 0 0 0 0 none none

Test results of the results of farming in another farm are shown in Table 13 and Table 14.

month Degree butterfat Somatic cells Effective bacterial count Collecting flow rate (ℓ) 1 week 2 weeks 1 week 2 weeks Ten thousand Rating January the first half 4.0 4.1 139 Grade 1 157 Grade 1 5 1A 9,435.1 January Second half 3.9 4.0 150 Grade 1 133 Grade 1 10 1A 10,259.5 February the first half 3.9 4.1 320 Grade 2 228 Grade 2 9 1A 9,049.5 February Second half 4.0 3.8 177 Grade 1 195 Grade 1 8 1A 8,249.7 In March the first half 4.1 4.1 114 Grade 1 149 Grade 1 18 1A 9,924.0 In March Second half 4.0 3.8 146 Grade 1 111 Grade 1 5 1A 10,822.0 April the first half 4.1 3.8 100 Grade 1 121 Grade 1 11 1A 10,257.4 April Second half 4.0 3.9 112 Grade 1 148 Grade 1 52 1B 10,827.0 In May the first half 4.0 4.0 179 Grade 1 240 Grade 2 8 1A 10,182.4 In May Second half 4.0 3.9 133 Grade 1 135 Grade 1 7 1A 10,679.3 June the first half 3.8 3.9 120 Grade 1 155 Grade 1 5 1A 9,446.0 June Second half 3.9 3.9 128 Grade 1 153 Grade 1 9 1A 9,429.6 First half subtotal 4.0 3.9 149 Grade 1 159 Grade 1 13 1A 118,606.5 In July the first half 4.0 3.9 241 Grade 2 186 Grade 1 9 1A 8,658.1 In July Second half 4.1 3.8 314 Grade 2 245 Grade 2 21 1A 8,755.9 August the first half 4.2 4.3 156 Grade 2 279 Grade 2 19 1A 7,781.8 August Second half 3.9 3.9 269 Grade 1 187 Grade 1 8 1A 9,241.8 September the first half 3.9 4.0 208 Grade 2 176 Grade 1 5 1A 8,823.9 September Second half 3.8 3.8 261 Grade 2 223 Grade 2 15 1A 8,525.5 October the first half 3.9 3.9 286 Grade 2 192 Grade 1 18 1A 8,553.0 October Second half 4.0 4.0 293 Grade 2 183 Grade 1 28 1A 10,208.4 November the first half 4.0 3.9 188 Grade 1 151 Grade 1 47 1B 8,891.7 November Second half 4.0 4.0 150 Grade 1 157 Grade 1 36 1B 9,356.7 December the first half 4.1 4.3 211 Grade 2 163 Grade 1 70 1B 9,593.2 December Second half 4.3 4.3 115 Grade 1 130 Grade 1 13 1A 10,352.3 Subtotal of the second half 4.0 4.0 224 Grade 2 186 Grade 1 24 1A 108,640.3 sum 4.0 4.0 185 Grade 1 172 Grade 1 18 1A 227,248.8

For milking, milk fat grades are shown in Table 14.

Measurement method: 5-point method, 1 (lowest) to 5 (highest) Reference value Farmhouse 3.8 ~ 4.2 3.8-5

Although the base value of milk fat is 3.8-4.2, the farmhouse using the present invention shows 3.8-5 points, indicating that the milk fat grade is high.

For milking, the number of somatic cells by grade is shown in Table 15.

Rating standard National average (%) Farmhouse One 200,000 31.2 92% 2 20-350,000 32.5 8% 3 35-500,000 17.8 0% 4 50-750,000 11.5 0% 5 More than 750,000 7% 0%

In the case of farms using the present invention, it can be seen that there is no grade 3 or less in the number of somatic cells.

In case of milking, the number of effective bacteria by grade is shown in Table 16.

Rating Bacteria National average Farm average 1A 1-3 thousand 79.4 99% 1B 3 ~ 100,000 14.9 1% other 2 10-250,000 3.8 0% 3 25-500,000 Etc 0% 4 500,000 or more Etc 0%

In the case of a farmhouse using the present invention, it can be seen that there are no grade 2 or less in the number of effective bacteria.

In the case of milking, the average fertility rate is 70%, while the fertility rate of farms using the fermented microbial feed of the present invention exceeds 90%.

In the present invention, the medium used for cultivating mushrooms is beneficial and has many nutrients, but it is difficult to digest and absorb livestock due to a large amount of fiber, but the fiber in the process of harvesting mycelia for use as livestock feed The fermented microbial feed can be manufactured using the destroyed mushroom medium, so that the polysaccharides that act as anticancer and immune regulators such as β-Glucan of the mushroom medium can be ingested by the livestock. It can be used as an adjuvant means to prevent mad cow disease by acting to prevent the proliferation and metastasis of cancer cells, significantly reduce the use of antibiotics, strengthen immunity to disease, improve feed efficiency, increase the body weight, It may have effects such as increased retention rate, increased reproduction success rate and odor removal.

In particular, the inoculation culture of lactic acid bacteria, yeast, actinomycetes, Bacillus bacteria, photosynthetic bacteria and the like as the main raw material of the mushroom medium is inoculated and cultured, and other microorganisms are killed by the lactic acid bacteria during the culture of the microorganisms. There is no use effect that can maximize the effect of the fermentation microorganisms.

In addition, when cultivation with normal feed, water should be added because the general feed has about 10% water content, but at least 6 hours are needed to make the optimized water content. In addition, since the moisture content of the mushroom medium has its own optimized water content of 50-70%, it is easy to culture, and there is also an effect on the manufacturing process that can shorten manufacturing costs and time.

Claims (5)

After harvesting bottle mushrooms, bag mushrooms, and box cultivated mushrooms, harvest and sanitize mushroom pads, separate and screen them, and then cultivate microbial agents such as yeast, lactic acid bacteria, Bacillus bacteria, photosynthetic bacteria and actinomycetes and mix them with general feed. In preparing fermented feeds: Culturing the lactic acid bacteria, yeast bacteria, Bacillus bacteria, photosynthetic bacteria, actinomycetes; 50 ~ 70% by weight of selected mushroom medium, 1 ~ 5% by weight of mushroom by-product, 15 ~ 35% by weight of moisture regulator made from rice bran and sweet rice, 5.5 ~ 15% by weight of corn flakes, 3 ~ 10% by weight of Pampit pellet, Adjusting the moisture of the feedstock such that the water content of the mixed feed of barley mixed pellet 2-8% by weight, hay 2-8% by weight, and trace ingredients 1.5-6% by weight is 25-78%; Lactic acid bacteria, lactic acid bacteria, yeast, Bacillus bacteria, photosynthetic bacteria, actinomycetes cultured in the liquid phase in the step using a inoculator injecting the selected liquid strain of 1 ~ 10ℓ per 1 ton of the feed material mixed with the moisture control agent to the mushroom medium in the step Obtaining a feed material inoculated with yeast, Bacillus bacteria, photosynthetic bacteria and actinomycetes, respectively; Mixing the microbial strain evenly in each feed material inoculated with the microbial strain in this step; Method of producing a fermentation microorganism feed additive for livestock using a mushroom medium, characterized in that the step of culturing the liquid strain mixed in each of the feedstock in the step can be directly fed to the livestock or used as a feed additive. After harvesting bottle mushrooms, bag mushrooms, and box cultivated mushrooms, harvest and sanitize mushroom pads, separate and screen them, and then cultivate microbial agents such as yeast, lactic acid bacteria, Bacillus bacteria, photosynthetic bacteria and actinomycetes and mix them with general feed. In preparing fermented feeds: Culturing the lactic acid bacteria, yeast bacteria, Bacillus bacteria, photosynthetic bacteria, actinomycetes; 50 ~ 70% by weight of selected mushroom medium, 1 ~ 5% by weight of mushroom by-product, 15 ~ 35% by weight of moisture regulator made from rice bran and sweet rice, 5.5 ~ 15% by weight of corn flakes, 3 ~ 10% by weight of Pampit pellet, Adjusting the moisture of the feedstock such that the water content of the mixed feed of barley mixed pellet 2-8% by weight, hay 2-8% by weight, and trace ingredients 1.5-6% by weight is 25-78%; For the feedstock obtained by mixing the moisture control agent in the mushroom medium in the step, the liquid strain cultured in the liquid state in the above step is divided into anaerobic fungi, which are lactic acid bacteria, yeast, Bacillus bacteria, photosynthetic bacteria, and aerobic fungi, which are actinomycetes. Injecting the anaerobic strain and aerobic strain 1 ~ 10ℓ per ton of each feedstock by using the inoculator to the feedstock to obtain two feedstocks inoculated with anaerobic strain and aerobic strain; Mixing the microbial strain evenly in each feed material inoculated with the microbial strain in this step; Method of producing a fermentation microorganism feed additive for livestock using a mushroom medium, characterized in that the step of culturing the liquid strain mixed in each of the feedstock in the step can be directly fed to the livestock or used as a feed additive. 50 to 70% by weight of mushroom medium, 1 to 5% by weight of mushroom by-product, 15 to 35% by weight of moisture control agent, 5.5 to 15% by weight of corn flakes, 3 to 10% by weight of pampite pellets, 2 to 8 weight of mixed pellets of wheat and barley Aerobic fungi and anaerobic fungi were mixed with 2% to 8% by weight of hay and 2% to 8% by weight of trace ingredients. Inoculate 1-10l per ton of feedstock and incubate them directly to feed animals or feed additives. Fermented microbial feed for livestock, characterized in that it can be used as. 10 to 30% by weight of hay, 5 to 15% by weight of corn flakes, 4 to 12% by weight of coconut shell pellets, cotton sheath, with 25 to 45% by weight of fermented microbial feed obtained by the process of claim 1 or 2 as an additive. 5 to 15% by weight, 5 to 14% by weight of corn stalks, 1 to 7% by molasses, 0.5 to 2% by weight of limestone, 0.5 to 2% by weight of salt, 1 to 3% by weight of a combination of vitamins and minerals Fermented microbial feed for livestock, characterized in that it can be used as useful. 10 to 30% by weight of hay, 5 to 15% by weight of corn flakes, 4 to 12% by weight of coconut shell pellets, wheat flour, using 25 to 45% by weight of the fermented microbial feed obtained by the process of claim 1 or 2 as an additive. 5 to 15% by weight, 5 to 14% by weight corn cobs, 1 to 7% by molasses, 0.5 to 2% by weight limestone, 0.5 to 2% by weight salt, 1 to 3% by weight of vitamin-mineral combination Fermented microbial feed for livestock, characterized in that it can be used for.

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