CN113812380A - Breeding method of hermetia illucens - Google Patents
Breeding method of hermetia illucens Download PDFInfo
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- CN113812380A CN113812380A CN202010562206.9A CN202010562206A CN113812380A CN 113812380 A CN113812380 A CN 113812380A CN 202010562206 A CN202010562206 A CN 202010562206A CN 113812380 A CN113812380 A CN 113812380A
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- feed
- hermetia illucens
- larvae
- pupae
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- 241000709785 Hermetia illucens Species 0.000 title claims abstract description 58
- 238000009395 breeding Methods 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 claims abstract description 23
- 230000001488 breeding effect Effects 0.000 claims abstract description 17
- 235000013601 eggs Nutrition 0.000 claims abstract description 8
- 238000005286 illumination Methods 0.000 claims abstract description 4
- 230000013011 mating Effects 0.000 claims abstract description 4
- 238000012258 culturing Methods 0.000 claims abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 238000011081 inoculation Methods 0.000 claims description 10
- 210000003608 fece Anatomy 0.000 claims description 8
- 241000238631 Hexapoda Species 0.000 claims description 6
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 claims description 3
- 241000382353 Pupa Species 0.000 claims description 3
- 240000008042 Zea mays Species 0.000 claims description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 3
- 235000005822 corn Nutrition 0.000 claims description 3
- 230000032669 eclosion Effects 0.000 claims description 3
- 230000003203 everyday effect Effects 0.000 claims description 3
- 235000012054 meals Nutrition 0.000 claims description 3
- 238000012827 research and development Methods 0.000 claims description 3
- 238000005303 weighing Methods 0.000 claims description 3
- 239000003973 paint Substances 0.000 claims description 2
- 238000005507 spraying Methods 0.000 claims description 2
- 240000004658 Medicago sativa Species 0.000 claims 1
- 230000012447 hatching Effects 0.000 abstract 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 11
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 11
- 239000010815 organic waste Substances 0.000 description 7
- 239000010871 livestock manure Substances 0.000 description 5
- 239000000463 material Substances 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 3
- 241000219823 Medicago Species 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241000255925 Diptera Species 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 235000021120 animal protein Nutrition 0.000 description 1
- 239000010828 animal waste Substances 0.000 description 1
- 235000021405 artificial diet Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000004634 feeding behavior Effects 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
- A23K10/38—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material from distillers' or brewers' waste
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a breeding method of hermetia illucens, which comprises the following steps: 1) collecting hermetia illucens wild population; 2) population feeding: inoculating the hermetia illucens larvae collected in the field into artificial feed in an artificial climate chamber to feed the hermetia illucens larvae to 6 days old; 3) inoculating the larvae into a target feed, and waiting for the larvae to be converted into pupae; 4) variety breeding: sorting the pupae fed from the target feed according to weight, selecting the pupae with the weight of 20 percent, collecting imagoes after the pupae emerge, mating the imagoes under the natural illumination condition to lay eggs, and hatching the eggs to obtain first generation of imagoes; 5) determination of new varieties: selecting the same target feed for the first generation of the hermetia illucens according to the method of the steps 2) to 4), and culturing the target feed until the tenth generation of the hermetia illucens is obtained, wherein the tenth generation of the hermetia illucens can be regarded as a new variety of the hermetia illucens aiming at the target feed. The method can be used for cultivating the new black soldier fly variety aiming at a certain target feed, and when the black soldier fly variety is bred by using the corresponding target feed, compared with the variety widely used at present, the weight of the larvae of the new variety is larger, the efficiency of treating the target feed is higher, and the method has good economic and social benefits.
Description
Technical Field
The invention belongs to the technical field of insect breeding, and particularly relates to a breeding method of hermetia illucens.
Background
Black soldier fly (Hermitia illucens L.) The name of the drug is hermetia illucens, which is an insect of hermetia illucens of hermetiaceae of diptera. The hermetia illucens have a wide feeding range, and the feeding behavior only occurs in a larva stage, and the larva naturally feeds on animal wastes, rotten organic matters (such as rotten meat, rotten fruits, vegetables and branches) and animal bodies.The digestive tract and symbiotic microorganisms of the hermetia illucens can efficiently decompose food ingested by the hermetia illucens for the growth and development of the body. At present, hermetia illucens is internationally used for treating various organic wastes, such as livestock and poultry manure, wine lees, agricultural product processing wastes and the like, and some countries utilize the hermetia illucens to produce animal protein for feed.
As an emerging breeding species, hermetia illucens do not have a breed for a specific feed at present. The varieties used at present are only American Texas varieties, Guangzhou varieties domesticated at southern China agricultural university and Wuhan varieties domesticated at Central China agricultural university, which are all derived from domestication of wild populations and are not further optimized for specific feeds. At present, feed for raising hermetia illucens is basically from organic waste in industrial and agricultural production and is characterized by a plurality of varieties. Each feed, such as bean dregs, has the characteristics of unique nutrient composition, moisture content, pH value and the like. An effective method for improving the treatment efficiency of the hermetia illucens on various organic wastes is to cultivate a new variety aiming at specific feed. Therefore, the cultivation of new black soldier fly species with independent intellectual property rights and the mastery of the core technology become the key of the high-point of the black soldier fly breeding industry.
At present, the work of breeding new black soldier fly varieties aiming at specific feed is not carried out in China.
Disclosure of Invention
The invention aims to solve the problem that no black soldier fly variety aiming at a specific feed exists at present, and provides a black soldier fly breeding method which is simple to operate, low in cost and short in period.
A method for breeding hermetia illucens comprises the following steps:
1) collecting field groups of hermetia illucens: collecting hermetia illucens larvae in the field, and bringing the hermetia illucens larvae back to a research and development base;
2) population feeding: in an artificial climate chamber, inoculating the larvae collected in the step 1) into artificial feed to feed the larvae to 6 days old, spraying water to a culture tray every day, and keeping the water content of the artificial feed at 70%;
3) inoculation to the target feed: separating the 6-day-old larvae obtained in the step 2) from the artificial feed, inoculating the larvae into the target feed, allowing the larvae to freely feed, and picking up pupae from the insect feces and the residual target feed after all the larvae are converted into the pupae;
4) variety breeding: selecting pupae with normal shape and large head, weighing by using a precision balance, properly marking all the weighed pupae, then sorting the pupae from large to small according to the weight, selecting pupae with the weight of 20 percent, collecting imagoes after the selected pupaes eclosion, waiting for the imagoes to lay eggs after mating under the natural illumination condition, and obtaining first generation of imagoes after the eggs are hatched;
5) determination of new varieties: selecting the same target feed for the first generation of the soldier fly according to the method described in the steps 2) to 4), generating the second generation of the soldier fly, and culturing until the tenth generation of the soldier fly is obtained, wherein the tenth generation of the soldier fly can be regarded as a new black soldier fly variety aiming at the target feed.
Further preferably, the climatic conditions of the artificial climate chamber in the step 2) are that the temperature is 25-30 ℃ and the relative humidity is 65-75%.
Further preferably, the artificial feed in step 2) is prepared from bran, alfalfa meal and corn distillers grains in a ratio of 5: 2: 3, and adding water to prepare the water-based paint, wherein the water content is 70-80%.
Further preferably, the inoculation amount of the inoculation in the step 2) is 1000-.
Further preferably, the inoculation amount of the inoculation in the step 3) is 500-.
Further preferably, the target feed of step 3) is a single component feed or a multi-component mixed feed.
Compared with the prior art, the breeding method of the hermetia illucens has at least the following beneficial effects or advantages.
(1) The invention provides a black soldier fly breeding method aiming at a specific target feed for the first time.
(2) The method can obtain a new black soldier fly variety, and when the black soldier fly is fed by using the corresponding target feed, compared with the variety widely used at present, the new black soldier fly variety has the advantages of larger individual weight of larvae, higher efficiency in treating the target feed and good economic and social benefits.
Detailed Description
The following describes in detail specific embodiments of the present invention. The embodiments described herein are exemplary only, and are not to be construed as limiting the invention.
The embodiment illustrates the implementation of the breeding method and the cultivated new black soldier fly variety aiming at the bean dregs, and simultaneously compares the processing capacity of the new variety on the bean dregs with that of the Wuhan variety.
Example 1: the breeding method aims at breeding a new black soldier fly variety of the bean dregs.
1) Collecting field groups of hermetia illucens: in suburbs or rural areas, where organic wastes are intensively stacked, such as chicken manure piles or cattle manure piles, robust, high-mobility and large-size larvae are searched and brought back to a research and development base.
2) Population feeding: in an artificial climate room with the temperature kept at 25-30 ℃ and the relative humidity of 65-75%, the larvae collected in the field are inoculated into artificial feed according to 2000 larvae per kg of artificial feed and fed to the age of 6 days, and a culture tray is sprayed with water every day to keep the water content of the artificial feed at 70%; the preparation method of the artificial feed comprises the following steps of mixing bran, alfalfa meal and corn vinasse by a weight ratio of 5: 2: 3, and adding water to prepare the mixture, so that the final water content is 70-80%.
3) Inoculating to bean dregs: separating 6-day-old larvae eating artificial feed from the artificial feed, inoculating 1000 heads/kg of bean dregs into the bean dregs, allowing the larvae to freely take food, and picking pupae from the insect manure and residual bean dregs after all the larvae are converted into the pupae; the okara was purchased from the market (water content 80%, pH = 6.2).
4) Variety breeding: selecting pupae with normal shape and large head, weighing by using a precision balance, properly marking all the weighed pupae, then sorting the pupae from large to small according to the weight, selecting pupae with the weight of 20 percent, collecting imagoes after the selected pupaes eclosion, waiting for the imagoes to lay eggs after mating under the natural illumination condition, and obtaining the first generation of imagoes after the eggs are hatched.
5) Determination of new varieties: and (3) selecting bean dregs as feed according to the method described in the steps 2) -4), generating second generation seed worms, and breeding to tenth generation seed worms, wherein the tenth generation seed worms can be regarded as a new black soldier fly variety aiming at the bean dregs and are named as BFL-DZ.
Example 2: and (4) comparing the hermetia illucens BFL-DZ variety with the Wuhan variety.
In the climatic chamber described in example 1, 6 day old BFL-DZ larvae and Wuhan variety larvae were fed with the artificial diet described in example 1, inoculated into 1kg of okara (water content 80%, pH = 6.2) at 1000 heads/kg of okara, allowed to feed freely, and after 50% of the larvae became pupated, pupae and larvae were picked out from the insect feces and residual okara. From the collected larvae, 300 heads were randomly selected, their total weight, total dry weight and average body weight, average dry weight were calculated. The residual worm manure and the residual bean dregs left after the larvae are harvested are used for analyzing the material reduction rate, the biological conversion rate and the like.
The average weight of the larvae is different between the two varieties, the average weight of the BFL-DZ variety is 27.3 percent higher than that of the Wuhan variety, and the average dry weight of the BFL-DZ variety is 35.9 percent higher than that of the Wuhan variety. This indicates that the BFL-DZ cultivar will have significant advantages when processing okara, see Table 1.
TABLE 1 comparison of Hermetia illucens BFL-DZ varieties with Wuhan variety larvae
Note: the number of experimental replicates = 3.
The material reduction rate and the biological conversion rate of the larvae between the two varieties are different, the material reduction rate of the BFL-DZ variety is 6.6 percent higher than that of the Wuhan variety, and the biological conversion rate of the BFL-DZ variety is 27.2 percent higher than that of the Wuhan variety. This indicates that the BFL-DZ cultivar will have greater processing capacity when processing okara, see Table 2.
TABLE 2 comparison of the reduction in materials and the bioconversion
Note: the number of experimental replicates = 3.
In conclusion, through the embodiment 1 and the embodiment 2, a new black soldier fly variety aiming at a target feed can be obtained according to the method, the new variety can harvest higher biomass of black soldier fly bodies, and meanwhile, the new variety has stronger processing capacity on the target feed.
The method can be widely applied to the treatment of organic wastes, and can promote the resource utilization of the black soldier fly to the organic wastes and reduce or eliminate the environmental pollution caused by the organic wastes by applying the new variety cultivated according to the method.
Claims (6)
1. A method for breeding hermetia illucens comprises the following steps:
1) collecting field groups of hermetia illucens: collecting hermetia illucens larvae in the field, and bringing the hermetia illucens larvae back to a research and development base;
2) population feeding: in an artificial climate chamber, inoculating the larvae collected in the step 1) into artificial feed to feed the larvae to 6 days old, spraying water to a culture tray every day, and keeping the water content of the artificial feed at 70%;
3) inoculation to the target feed: separating the 6-day-old larvae obtained in the step 2) from the artificial feed, inoculating the larvae into the target feed, allowing the larvae to freely feed, and picking up pupae from the insect feces and the residual target feed after all the larvae are converted into the pupae;
4) variety breeding: selecting pupae with normal shape and large head, weighing by using a precision balance, properly marking all the weighed pupae, then sorting the pupae from large to small according to the weight, selecting pupae with the weight of 20 percent, collecting imagoes after the selected pupaes eclosion, waiting for the imagoes to lay eggs after mating under the natural illumination condition, and obtaining first generation of imagoes after the eggs are hatched;
5) determination of new varieties: selecting the same target feed for the first generation of the soldier fly according to the method described in the steps 2) to 4), generating the second generation of the soldier fly, and culturing until the tenth generation of the soldier fly is obtained, wherein the tenth generation of the soldier fly can be regarded as a new black soldier fly variety aiming at the target feed.
2. The method of breeding hermetia illucens according to claim 1, wherein the method comprises the following steps: the climatic conditions of the artificial climatic chamber in the step 2) are that the temperature is 25-30 ℃ and the relative humidity is 65-75%.
3. The method of breeding hermetia illucens according to claim 1, wherein the method comprises the following steps: the artificial feed in the step 2) is prepared from bran, alfalfa meal and corn vinasse in a ratio of 5: 2: 3, and adding water to prepare the water-based paint, wherein the water content is 70-80%.
4. The method of breeding hermetia illucens according to claim 1, wherein the method comprises the following steps: the inoculation amount of the inoculation in the step 2) is 1000-3000 heads/kg of artificial feed.
5. The method of breeding hermetia illucens according to claim 1, wherein the method comprises the following steps: the inoculation amount of the inoculation in the step 3) is 500-1500 heads/kg of target feed.
6. The method of breeding hermetia illucens according to claim 1, wherein the method comprises the following steps: the target feed in the step 3) is single-component feed or multi-component mixed feed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010562206.9A CN113812380A (en) | 2020-06-19 | 2020-06-19 | Breeding method of hermetia illucens |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010562206.9A CN113812380A (en) | 2020-06-19 | 2020-06-19 | Breeding method of hermetia illucens |
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| Publication Number | Publication Date |
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| CN113812380A true CN113812380A (en) | 2021-12-21 |
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| CN202010562206.9A Pending CN113812380A (en) | 2020-06-19 | 2020-06-19 | Breeding method of hermetia illucens |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115067285A (en) * | 2022-07-21 | 2022-09-20 | 上海市农业科学院 | Method for obtaining platysternon megacephalum breeding harlequin fleahopper harleys by using wild rice stem straws |
| CN115351055A (en) * | 2022-08-18 | 2022-11-18 | 长沙中联重科环境产业有限公司 | Method for converting kitchen waste by utilizing stratiomyiid |
| CN115716766A (en) * | 2022-11-26 | 2023-02-28 | 广西农科环保科技有限公司 | Black soldier fly sand high-grade nutrient fertilizer capable of treating ginger blast disease |
-
2020
- 2020-06-19 CN CN202010562206.9A patent/CN113812380A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115067285A (en) * | 2022-07-21 | 2022-09-20 | 上海市农业科学院 | Method for obtaining platysternon megacephalum breeding harlequin fleahopper harleys by using wild rice stem straws |
| CN115067285B (en) * | 2022-07-21 | 2023-12-05 | 上海市农业科学院 | Method for obtaining white star flower scarab breeding furgus lucorum by using cane shoot straw |
| CN115351055A (en) * | 2022-08-18 | 2022-11-18 | 长沙中联重科环境产业有限公司 | Method for converting kitchen waste by utilizing stratiomyiid |
| CN115716766A (en) * | 2022-11-26 | 2023-02-28 | 广西农科环保科技有限公司 | Black soldier fly sand high-grade nutrient fertilizer capable of treating ginger blast disease |
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Application publication date: 20211221 |