CN115316570B - Biological feed for culturing hermetia illucens and preparation method thereof - Google Patents
Biological feed for culturing hermetia illucens and preparation method thereof Download PDFInfo
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- 241000709785 Hermetia illucens Species 0.000 title claims abstract description 55
- 238000012258 culturing Methods 0.000 title claims abstract description 33
- 238000002360 preparation method Methods 0.000 title abstract description 10
- 239000007788 liquid Substances 0.000 claims abstract description 72
- 235000002566 Capsicum Nutrition 0.000 claims abstract description 46
- 239000006002 Pepper Substances 0.000 claims abstract description 46
- 241000722363 Piper Species 0.000 claims abstract description 46
- 235000016761 Piper aduncum Nutrition 0.000 claims abstract description 46
- 235000017804 Piper guineense Nutrition 0.000 claims abstract description 46
- 235000008184 Piper nigrum Nutrition 0.000 claims abstract description 46
- 239000010871 livestock manure Substances 0.000 claims abstract description 44
- 230000001580 bacterial effect Effects 0.000 claims abstract description 43
- 210000003608 fece Anatomy 0.000 claims abstract description 43
- 239000006041 probiotic Substances 0.000 claims abstract description 41
- 235000018291 probiotics Nutrition 0.000 claims abstract description 41
- 241000287828 Gallus gallus Species 0.000 claims abstract description 40
- 241000894006 Bacteria Species 0.000 claims abstract description 27
- 230000000529 probiotic effect Effects 0.000 claims abstract description 26
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 23
- 101000722833 Geobacillus stearothermophilus 30S ribosomal protein S16 Proteins 0.000 claims abstract description 22
- 241001468157 Lactobacillus johnsonii Species 0.000 claims abstract description 22
- 239000002131 composite material Substances 0.000 claims abstract description 13
- 239000000203 mixture Substances 0.000 claims description 19
- 238000002156 mixing Methods 0.000 claims description 15
- 238000000034 method Methods 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 4
- 238000007873 sieving Methods 0.000 claims description 4
- 239000000463 material Substances 0.000 abstract description 17
- 238000000855 fermentation Methods 0.000 abstract description 13
- 230000004151 fermentation Effects 0.000 abstract description 13
- 241000345998 Calamus manan Species 0.000 abstract description 12
- 235000012950 rattan cane Nutrition 0.000 abstract description 12
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- 235000016709 nutrition Nutrition 0.000 abstract description 3
- 235000019750 Crude protein Nutrition 0.000 abstract description 2
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- 150000002215 flavonoids Chemical class 0.000 abstract description 2
- 235000017173 flavonoids Nutrition 0.000 abstract description 2
- 230000036039 immunity Effects 0.000 abstract description 2
- 230000035764 nutrition Effects 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 239000000341 volatile oil Substances 0.000 abstract description 2
- 238000011282 treatment Methods 0.000 description 13
- 241000238631 Hexapoda Species 0.000 description 12
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- 238000006243 chemical reaction Methods 0.000 description 7
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- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 4
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- 240000007594 Oryza sativa Species 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
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- 235000009566 rice Nutrition 0.000 description 3
- 239000002028 Biomass Substances 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- 241000233805 Phoenix Species 0.000 description 1
- 241000011500 Phyllostachys praecox Species 0.000 description 1
- 235000008545 Phyllostachys praecox Nutrition 0.000 description 1
- 241000254109 Tenebrio molitor Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
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- 238000003306 harvesting Methods 0.000 description 1
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- 108090000623 proteins and genes Proteins 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/151—Johnsonii
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention relates to a biological feed for culturing hermetia illucens and a preparation method thereof, comprising the following components: fresh chicken manure, rattan pepper leaves and probiotic bacteria liquid, wherein the probiotic bacteria liquid accounts for 1-10% of the total mass of the fresh chicken manure and rattan pepper leaves, and the probiotic bacteria liquid at least contains lactobacillus johnsonii BS15 bacteria liquid. The invention adopts bacillus licheniformis and lactobacillus johnsonii BS15 to prepare the composite bacteria bacterial liquid according to the equal proportion, has good fermentation effect, does not inhibit the other side, has good decomposition effect on fermentation materials, saves auxiliary materials and has short fermentation time. The fermentation and decomposition of the compound probiotics can effectively decompose crude proteins, crude fats and crude fibers in chicken manure and the rattan pepper leaves to meet the nutrition requirements of the black soldier fly larvae, and after the fermentation of the compound probiotics, flavonoid substances and volatile oil in the rattan pepper leaves can inhibit harmful bacteria, so that animal immunity is improved, diseases are reduced, and the growth of the black soldier fly larvae is promoted, so that the survival rate of the black soldier fly larvae is improved.
Description
Technical Field
The invention relates to a feed, in particular to a biological feed for culturing hermetia illucens and a preparation method thereof.
Background
The black soldier fly (Hermetia illucens L.), the saprophytic soldier fly can eat livestock manure and household garbage, and high-value animal protein feed is produced, so that the black soldier fly is utilized as resources due to the characteristics of rapid propagation, large biomass, wide feeding range, high absorption and conversion rate, easy management, low feeding cost, good palatability of animals and the like, and the larvae are called as phoenix insects and become resource insects with the same names as fly maggots, yellow mealworms, barley insects and the like, so that the black soldier fly and the like are popularized worldwide. The hermetia illucens is native in the america and is widely distributed worldwide (between 40 degrees in north and south latitude). In recent years, the Chinese medicinal materials are widely spread in Guizhou, guangxi, guangdong, shanghai, yunnan, taiwan, hunan, hubei and other places. Is widely applied to the aspect of treating wastes such as chicken manure, pig manure, fresh chicken manure and the like. However, the problem of slow growth of the black soldier fly larvae can be caused by directly feeding the black soldier fly with chicken manure, and for this reason, the Chinese patent application with publication number of CN113925023A, named the method for feeding the commercial black soldier fly larvae by using the mixture of chicken manure and phyllostachys praecox as feed, discloses a preparation method of the growth feed for the black soldier fly larvae, which is to add rice bran or rice fine hulls, she Caoxian grass paddles, a small amount of lactic acid bacteria and a fecal feed starter into the chicken manure, so as to prepare the feed for the black soldier fly larvae. The feed solves the problem of low protein content in the chicken manure due to the defect of nutritional ingredients of the black soldier fly larvae fed by the pure chicken manure. However, the hermetia illucens frequently suffers from diseases due to density or environmental reasons in the cultivation process, and the diseases can cause the reduction of the survival rate of the larvae of the hermetia illucens.
The vine pepper leaves have special spicy smell similar to the vine pepper peel, and are byproducts of the vine pepper industry. The rattan pepper branches and leaves cut in rattan pepper planting management and rattan pepper branches and leaves cut during rattan pepper harvesting are usually burnt or buried as waste, are not reasonably utilized, and cause resource waste and even pollute the environment.
Disclosure of Invention
In view of the above, it is necessary to provide a biological feed for raising hermetia illucens and a preparation method thereof. The technical scheme of the invention is as follows:
in a first aspect, the invention provides a biological feed for culturing hermetia illucens, which comprises the following components:
fresh chicken manure, rattan pepper leaves and probiotic bacteria liquid, wherein the probiotic bacteria liquid accounts for 1-10% of the total mass of the fresh chicken manure and rattan pepper leaves, and the probiotic bacteria liquid at least contains lactobacillus johnsonii BS15 bacteria liquid.
Preferably, the fresh chicken manure and the vine pepper leaves are mixed according to the following weight percentage: 70-90%: 10-30%.
More preferably, the fresh chicken manure and the vine pepper leaves are mixed according to the following weight percentage: 80%:20%.
Preferably, the probiotic bacterial liquid is formed by mixing lactobacillus johnsonii BS15 bacterial liquid and bacillus licheniformis H2 bacterial liquid according to a mass ratio of 1:1.
Preferably, the addition amount of the probiotic bacteria liquid is 1-3% of the total mass of the fresh chicken manure and the vine pepper leaves.
Preferably, the water content of the biological feed for culturing the hermetia illucens is controlled to be 55-60%.
In a second aspect, the invention provides a preparation method of a biological feed for culturing hermetia illucens, which comprises the following steps:
(1) uniformly mixing the fresh chicken manure and the vine pepper leaves according to the weight percentage to obtain a mixture;
(2) and culturing the probiotics with a culture medium to form a probiotic bacterial liquid, sprinkling the probiotic bacterial liquid on the mixture according to the proportion, and uniformly mixing to obtain the biological feed for culturing the hermetia illucens.
Preferably, the vine pepper leaves are dried, crushed and sieved by a 60-mesh sieve.
Preferably, the step (2) includes the steps of:
culturing Lactobacillus johnsonii BS15 and Bacillus licheniformis H2 with culture medium to obtain Lactobacillus johnsonii BS15 bacterial solution and Bacillus licheniformis H2 bacterial solution, mixing, and making into 1×10 5 ～1×10 8 And (3) the CFU/ml composite probiotic bacteria liquid is sprayed on the mixture, and then the mixture is uniformly mixed.
The invention has the advantages and beneficial effects that:
the biological feed for culturing the hermetia illucens comprises fresh chicken manure, vine pepper leaf powder and composite probiotic bacteria liquid, wherein the fresh chicken manure is used as a main material, the vine pepper leaf and the composite probiotic bacteria liquid are used as auxiliary materials, and the chicken manure and the vine pepper leaf are both biomass waste, so that the full utilization of waste is realized.
The composite bacterial liquid is prepared by mixing the bacillus licheniformis bacterial liquid and the lactobacillus johnsonii BS15 bacterial liquid according to equal proportion, has good fermentation effect, does not inhibit the other side, has good decomposition effect on fermentation materials, saves auxiliary materials and has short fermentation time. The fermentation and decomposition of the compound probiotics can effectively decompose crude proteins, crude fats and crude fibers in chicken manure and the rattan pepper leaves to meet the nutrition requirements of the black soldier fly larvae, and after the fermentation of the compound probiotics, flavonoid substances and volatile oil in the rattan pepper leaves can inhibit harmful bacteria, so that animal immunity is improved, diseases are reduced, and the growth of the black soldier fly larvae is promoted, so that the survival rate of the black soldier fly larvae is improved.
Detailed Description
The present invention will be described in further detail with reference to the following examples in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the present invention.
Example 1:
the preparation method of the biological feed for culturing hermetia illucens comprises the following steps:
(1) drying and pulverizing the vine pepper leaves, and sieving with a 60-mesh sieve. Mixing 80 parts by weight of fresh chicken manure and 20 parts by weight of crushed vine pepper leaves uniformly to obtain a mixture.
(2) The preserved Lactobacillus johnsonii BS15 was cultured in an incubator at 37℃for 12 hours using 10ml of liquid MRS medium per 10. Mu.L of the extract to obtain a Lactobacillus johnsonii BS15 bacterial liquid. Culturing the preserved Bacillus licheniformis H2 in 10ml LB liquid medium per 10 μl, at 37deg.C in incubator for 15 hr to obtain Bacillus licheniformis H2 bacterial liquid, mixing 1 part by weight of Lactobacillus johnsonii BS15 bacterial liquid with 1 part by weight of Bacillus licheniformis H2 bacterial liquid to obtain 1×10 concentration 8 CFU/ml composite probiotic bacteria liquid is sprayed on the mixture and mixedAfter being uniform, the mixture is kept at 35 ℃ and sealed for 10 hours, so as to obtain the biological feed for culturing the hermetia illucens; the water content of the biological feed for culturing the hermetia illucens is controlled to be 55%.
Example 2:
the preparation method of the biological feed for culturing hermetia illucens comprises the following steps:
(1) drying and pulverizing the vine pepper leaves, and sieving with a 60-mesh sieve. Mixing 90 parts by weight of fresh chicken manure and 10 parts by weight of crushed vine pepper leaves uniformly to obtain a mixture.
(2) The preserved Lactobacillus johnsonii BS15 was cultured in an incubator at 37℃for 12 hours using 10ml of liquid MRS medium per 10. Mu.L of the extract to obtain a Lactobacillus johnsonii BS15 bacterial liquid. Culturing the preserved Bacillus licheniformis H2 in 10ml LB liquid medium per 10 μl, at 37deg.C in incubator for 15 hr to obtain Bacillus licheniformis H2 bacterial liquid, mixing 0.5 part by weight of Lactobacillus johnsonii BS15 bacterial liquid with 0.5 part by weight of Bacillus licheniformis H2 bacterial liquid to obtain 1×10 concentration 7 The CFU/ml composite probiotic bacteria liquid is sprayed on the mixture, and after being uniformly mixed, the mixture is kept at 30 ℃ for sealing for 12 hours, so as to obtain the biological feed for culturing the hermetia illucens; the water content of the biological feed for culturing the hermetia illucens is controlled to be 55%.
Example 3:
the preparation method of the biological feed for culturing hermetia illucens comprises the following steps:
(1) drying and pulverizing the vine pepper leaves, and sieving with a 60-mesh sieve. And uniformly mixing 70 parts by weight of fresh chicken manure and 30 parts by weight of crushed vine pepper leaves to obtain a mixture.
(2) The preserved Lactobacillus johnsonii BS15 was cultured in an incubator at 37℃for 12 hours using 10ml of liquid MRS medium per 10. Mu.L of the extract to obtain a Lactobacillus johnsonii BS15 bacterial liquid. Culturing the preserved Bacillus licheniformis H2 in 10ml LB liquid medium per 10 μl, at 37deg.C in incubator for 15 hr to obtain Bacillus licheniformis H2 bacterial liquid, mixing 1.5 weight parts of Lactobacillus johnsonii BS15 bacterial liquid with 1.5 weight parts of Bacillus licheniformis H2 bacterial liquid to obtain 1×10 concentration 5 The CFU/ml composite probiotic bacteria liquid is sprayed on the mixture,after being uniformly mixed, the mixture is kept at 32 ℃ and sealed for 8 hours, so as to obtain the biological feed for culturing the hermetia illucens; the water content of the biological feed for culturing the hermetia illucens is controlled to be 60%.
Experimental example:
1. and (3) raising and managing hermetia illucens:
before the test, the hatched larvae are fed into the feed fermented by corn bran and rice chaff and added with a small amount of brown sugar, and after 4 days of feeding, the larvae are respectively added into 1m according to 4kg (about 80 ten thousand) larvae 3 In a volumetric cement pit. And (3) observing the feeding condition of the hermetia illucens larvae in the test process, and starting feeding when feeding is finished, wherein the feeding finishing standard is that the color of the feed changes and the texture is fluffy. And (3) beginning to collect insects in the morning after the insects are collected, separating the insects and the manure by adopting a vibration classifying screen, and weighing the fresh insects and the manure respectively. The culture room temperature is kept between 25 and 28 ℃ and the humidity is kept between 65 and 70 percent in the culture process. The light cycle (light: dark) during the test period was 16h:8h.
2. The mixture ratio of the main materials and the auxiliary materials of the hermetia illucens biological feed:
the main material of the biological feed formula for culturing the hermetia illucens is fresh chicken manure, and vine pepper leaves and compound probiotics bacterial liquid are respectively used as auxiliary materials. The composite probiotics bacterial liquid is prepared from lactobacillus johnsonii BS15 bacterial liquid and bacillus licheniformis bacterial liquid according to the mass ratio of 1: 1. The main materials and the auxiliary materials were mixed in proportion, 16 experimental treatments were set up and 4 comparative treatments were set up, wherein the 16 experimental treatments were divided into four groups as shown in table 1: the first group is a group without probiotics, and the ratio of the fresh chicken manure to the vine pepper leaves in the four embodiments is 100 parts by weight respectively: 0 parts by weight, 90 parts by weight: 10 parts by weight, 80 parts by weight: 20 parts by weight, 70 parts by weight: 30 parts by weight; the second group is that the addition amount of the compound probiotics bacterial liquid is 1% of the total mass of the fresh chicken manure and the vine pepper leaves, and the ratio of the fresh chicken manure to the vine pepper leaves in the four experimental examples is 100 parts by weight respectively: 0 parts by weight, 90 parts by weight: 10 parts by weight, 80 parts by weight: 20 parts by weight, 70 parts by weight: 30 parts by weight; the third group is that the addition amount of the compound probiotics bacterial liquid is 2% of the total mass of the fresh chicken manure and the vine pepper leaves, and the ratio of the fresh chicken manure to the vine pepper leaves in the four experimental examples is 100 parts by weight respectively: 0 parts by weight, 90 parts by weight: 10 parts by weight, 80 parts by weight: 20 parts by weight, 70 parts by weight: 30 parts by weight; the fourth group is that the addition amount of the compound probiotics bacterial liquid is 3% of the total mass of the fresh chicken manure and the vine pepper leaves, and the ratio of the fresh chicken manure to the vine pepper leaves in the four experimental examples is 100 parts by weight respectively: 0 parts by weight, 90 parts by weight: 10 parts by weight, 80 parts by weight: 20 parts by weight, 70 parts by weight: 30 parts by weight. Comparative examples 1-4 treatments the biological feed moisture content was controlled to 55% and the total mass and survival rate of the harvested larvae was counted after 9 days as shown in table 2.
TABLE 1 proportions of major and minor ingredients of hermetia illucens biological feed
Table 2 proportion of Main Material and auxiliary Material of comparative examples 1 to 4
Table 3 influence of different main materials, auxiliary materials, probiotic liquid on the growth performance of hermetia illucens larvae n=4
As can be seen from Table 3, the insect-receiving quality and the insect-receiving quality of the experimental examples 6-8, 10-12 and 14-16 are not significantly different (P > 0.05). Comparison of larval yield, experimental examples 11 and 15 treat both groups significantly higher than the other groups, and the feed to insect ratio was significantly lower than the other groups (P < 0.05). Survival rate comparison, treatment of experimental examples 11, 14, 15, 16 was significantly higher than other treatments (P < 0.05). Dry matter conversion was compared, the 11 th, 14 th, 15 th experimental example treatments were significantly higher than the other treatments (P < 0.05). The most cost-saving probiotic bacteria liquid is added from the economic benefit, and the ratio of fresh chicken manure to vine pepper leaves is 80 percent by weight: the addition amount of the 20% of the probiotic bacteria liquid is 2% of the total mass of the fresh chicken manure and the vine pepper leaves, and the probiotic bacteria liquid is the optimal proportion.
The treatment raw material of the 1 st experimental example is only fresh chicken manure, the larva yield, the survival rate and the dry matter conversion rate of the chicken manure are all obviously lower than those of the 6 th to 8 th experimental examples, the 10 th to 12 th experimental examples and the 14 th to 16 th experimental examples, and the feed worm ratio is obviously higher than those of the 6 th to 8 th experimental examples, the 10 th to 12 th experimental examples and the 14 th to 16 th experimental examples.
The 2 nd to 4 th experimental examples are treated without adding composite probiotics bacterial liquid, the larva yield, survival rate and dry matter conversion rate are all obviously lower than those of the 6 th to 8 th experimental examples, the 10 th to 12 th experimental examples and the 14 th to 16 th experimental examples, and the feed and insect ratio is obviously higher than those of the 6 th to 8 th experimental examples, the 10 th to 12 th experimental examples and the 14 th to 16 th experimental examples.
The treatment of the experimental examples 5, 9 and 13 is that the larva yield, the survival rate and the dry matter conversion rate of the treatment are all obviously lower than those of the treatment of the experimental examples 6-8, 10-12 and 14-16, and the feed and insect ratio is obviously higher than those of the treatment of the experimental examples 6-8, 10-12 and 14-16.
In comparative example 1, the stem pepper leaves are replaced by corn stalks, and the corn stalks contain more than 30% of carbohydrate, 2% -4% of protein and 0.5% -1% of fat, but can only provide pure taste nutrients, the corn stalks are matched with other raw materials, so that the disease prevention effect on the black soldier fly is not achieved, and harmful bacteria such as mould and the like are often present in the natural source stalks, so that the survival rate of the black soldier fly is reduced.
In comparative example 2, the composite probiotic bacteria liquid is replaced by the bacillus licheniformis bacteria liquid, the fermentation effect of the bacillus licheniformis bacteria liquid on biological feed is limited, and the promotion effect on the yield and survival rate of the hermetia illucens larvae is poor.
In comparative example 3, after the bacillus licheniformis bacteria liquid in the composite probiotic bacteria liquid is changed into the bacillus subtilis bacteria liquid, the larva pest yield, the survival rate and the dry matter conversion rate of comparative example 3 are obviously reduced, and the feed worm ratio is increased.
The mass ratio of the lactobacillus johnsonii BS15 bacterial liquid to the bacillus licheniformis H2 bacterial liquid in the comparative example 4 is 1:3, after the proportion of the bacillus licheniformis H2 bacterial liquid is increased, the fermentation effect of the lactobacillus johnsonii BS15 bacterial liquid is possibly interfered, so that the overall fermentation effect is influenced, the larva insect yield, the survival rate and the dry matter conversion rate are obviously reduced, and the feed and insect ratio is increased.
The foregoing examples illustrate only a few embodiments of the invention and are described in detail herein without thereby limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.
Claims (6)
1. The biological feed for culturing the hermetia illucens is characterized by comprising the following components:
fresh chicken manure, vine pepper leaves and probiotics bacterial liquid,
the fresh chicken manure and the vine pepper leaves are mixed according to the weight percentage: 70-90%: 10-30%;
the probiotics bacterial liquid is formed by mixing lactobacillus johnsonii BS15 bacterial liquid and bacillus licheniformis H2 bacterial liquid according to a mass ratio of 1:1;
the addition amount of the probiotic bacteria liquid is 1-3% of the total mass of the fresh chicken manure and the vine pepper leaves.
2. The biological feed for culturing hermetia illucens according to claim 1, wherein: the fresh chicken manure and the vine pepper leaves are mixed according to the weight percentage: 80%:20%.
3. The biological feed for culturing hermetia illucens according to claim 1, wherein: the water content of the biological feed for culturing the hermetia illucens is controlled to be 55-60%.
4. A method for preparing the biological feed for raising hermetia illucens according to any one of claims 1-3, comprising the following steps:
(1) uniformly mixing fresh chicken manure and vine pepper leaves according to weight percentage to obtain a mixture;
(2) and culturing the probiotics with a culture medium to form a probiotic bacterial liquid, sprinkling the probiotic bacterial liquid on the mixture according to a proportion, and uniformly mixing to obtain the biological feed for culturing the hermetia illucens.
5. The method for preparing the biological feed for culturing hermetia illucens according to claim 4, which is characterized in that: the step (1) further comprises: and (3) drying the vine pepper leaves, crushing, and sieving with a 60-mesh sieve.
6. The method for preparing the biological feed for culturing hermetia illucens according to claim 4, which is characterized in that: the step (2) comprises the following steps:
culturing Lactobacillus johnsonii BS15 and Bacillus licheniformis H2 with culture medium to obtain Lactobacillus johnsonii BS15 bacterial solution and Bacillus licheniformis H2 bacterial solution, mixing, and making into 1×10 5 ～1×10 8 And (3) the CFU/ml composite probiotic bacteria liquid is sprayed on the mixture, and then the mixture is uniformly mixed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210960882.0A CN115316570B (en) | 2022-08-11 | 2022-08-11 | Biological feed for culturing hermetia illucens and preparation method thereof |
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Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103243041A (en) * | 2012-07-27 | 2013-08-14 | 四川农业大学 | Bacillus licheniformis H2 and application thereof |
| KR20140054531A (en) * | 2012-10-29 | 2014-05-09 | 공영경 | Feedstuff for carnivorous freshwater fish farming and its manufacturing method |
| CN105433066A (en) * | 2015-12-25 | 2016-03-30 | 广东省农业科学院蚕业与农产品加工研究所 | Method for producing insect protein feed by treating excrements of livestock by virtue of Hermetia illucens |
| CN105941975A (en) * | 2016-05-20 | 2016-09-21 | 四川农业大学 | Broiler feed on basis of lactobacillus johnsonii BS15 and method for preparing broiler feed |
| CN106387354A (en) * | 2016-08-31 | 2017-02-15 | 四川农业大学 | Novel tenebrio molitor bio-fermented feed and preparation method thereof |
| CN107347804A (en) * | 2017-08-28 | 2017-11-17 | 宁夏甏菜文化传播有限公司 | Utilize black soldier flies and the method for the green organic chicken of source bacterium cyclic culture |
| CN108849770A (en) * | 2018-08-20 | 2018-11-23 | 四川鑫诚发畜牧机械有限公司 | Fowl and animal excrement automated processing equipment based on black soldier flies raising |
| CN109090043A (en) * | 2018-07-30 | 2018-12-28 | 开平市华声生物科技有限公司 | A kind of black soldier flies larva cultural method |
| CN109757627A (en) * | 2019-03-26 | 2019-05-17 | 四川十分美环境卫生管理有限公司 | A kind of feed and preparation method that can promote the growth of black soldier flies larva |
| CN113018320A (en) * | 2021-02-04 | 2021-06-25 | 四川农业大学 | Application of lactobacillus johnsonii BS15 in preparation of medicine for preventing and/or treating intestinal injury caused by chronic fluorosis |
| CN113925023A (en) * | 2021-10-18 | 2022-01-14 | 江苏函夏新农业科技有限公司 | Method for breeding black soldier fly commercial larvae by mixing chicken manure and lysimachia foenum-graecum as feed |
| CN114431335A (en) * | 2022-02-22 | 2022-05-06 | 黑龙江省科学院高技术研究院 | Biological feed for breeding hermetia illucens |
-
2022
- 2022-08-11 CN CN202210960882.0A patent/CN115316570B/en active Active
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103243041A (en) * | 2012-07-27 | 2013-08-14 | 四川农业大学 | Bacillus licheniformis H2 and application thereof |
| KR20140054531A (en) * | 2012-10-29 | 2014-05-09 | 공영경 | Feedstuff for carnivorous freshwater fish farming and its manufacturing method |
| CN105433066A (en) * | 2015-12-25 | 2016-03-30 | 广东省农业科学院蚕业与农产品加工研究所 | Method for producing insect protein feed by treating excrements of livestock by virtue of Hermetia illucens |
| CN105941975A (en) * | 2016-05-20 | 2016-09-21 | 四川农业大学 | Broiler feed on basis of lactobacillus johnsonii BS15 and method for preparing broiler feed |
| CN106387354A (en) * | 2016-08-31 | 2017-02-15 | 四川农业大学 | Novel tenebrio molitor bio-fermented feed and preparation method thereof |
| CN107347804A (en) * | 2017-08-28 | 2017-11-17 | 宁夏甏菜文化传播有限公司 | Utilize black soldier flies and the method for the green organic chicken of source bacterium cyclic culture |
| CN109090043A (en) * | 2018-07-30 | 2018-12-28 | 开平市华声生物科技有限公司 | A kind of black soldier flies larva cultural method |
| CN108849770A (en) * | 2018-08-20 | 2018-11-23 | 四川鑫诚发畜牧机械有限公司 | Fowl and animal excrement automated processing equipment based on black soldier flies raising |
| CN109757627A (en) * | 2019-03-26 | 2019-05-17 | 四川十分美环境卫生管理有限公司 | A kind of feed and preparation method that can promote the growth of black soldier flies larva |
| CN113018320A (en) * | 2021-02-04 | 2021-06-25 | 四川农业大学 | Application of lactobacillus johnsonii BS15 in preparation of medicine for preventing and/or treating intestinal injury caused by chronic fluorosis |
| CN113925023A (en) * | 2021-10-18 | 2022-01-14 | 江苏函夏新农业科技有限公司 | Method for breeding black soldier fly commercial larvae by mixing chicken manure and lysimachia foenum-graecum as feed |
| CN114431335A (en) * | 2022-02-22 | 2022-05-06 | 黑龙江省科学院高技术研究院 | Biological feed for breeding hermetia illucens |
Non-Patent Citations (1)
| Title |
|---|
| A VALUE A D D E D M A N U R E M A N A G E M E N T SYSTEM USING THE BLACK SOLDIER FLY;D. Craig Sheppard;《Bioresource Technology 》;第50卷;第275-279页 * |
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