CN108541806B - Seed melon peel and pulp fermentation type compound feed and preparation method thereof - Google Patents

Seed melon peel and pulp fermentation type compound feed and preparation method thereof Download PDF

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CN108541806B
CN108541806B CN201810273515.7A CN201810273515A CN108541806B CN 108541806 B CN108541806 B CN 108541806B CN 201810273515 A CN201810273515 A CN 201810273515A CN 108541806 B CN108541806 B CN 108541806B
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seed melon
pulp
feed
melon peel
fermentation
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CN108541806A (en
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买尔哈巴·艾合买提
杨新平
艾尼江·尔斯曼
冯蕾
陈竞
代金平
古丽·艾合买提
秦新政
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Institute Of Microbial Applications Xinjiang Academy Of Agricultural Sciences (china Xinjiang-Armenia Bioengineering Research And Development Center)
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Institute Of Microbial Applications Xinjiang Academy Of Agricultural Sciences (china Xinjiang-Armenia Bioengineering Research And Development Center)
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/10Feeding-stuffs specially adapted for particular animals for ruminants
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass

Abstract

The invention provides a seed melon peel and pulp fermented compound feed, which comprises the following specific preparation steps: (1) pulping the seed melon pulp, and crushing seed melon peel into particles of 1-2mm for later use; then crushing the dry wheat straw into 2-3mm and crushing the soybean meal into 200-300 mu m for later use; (2) mixing seed melon peel and pulp, dry wheat straw and soybean meal according to a weight ratio of 7:2:1, scattering a fermentation microbial inoculum according to a weight ratio of 2%, fully and uniformly stirring, mixing fermentation raw materials, filling the mixture into a fermentation bag in an amount of 30 Kg/bag, and fermenting at an initial fermentation temperature of lower than 27 ℃ and a temperature of not higher than 41 ℃ during fermentation for 30 days to open the bag for feeding. The content of 17 amino acids in the feed obtained by the invention is averagely improved by 9.1 percent compared with the content of the amino acids in the raw materials, and the fermented feed has remarkable feed utilization rate and remarkable feeding effect when being applied to feeding Xinjiang fine wool sheep, Aletai sheep and Xinjiang brown cattle.

Description

Seed melon peel and pulp fermentation type compound feed and preparation method thereof
Technical Field
The invention belongs to the technical field of livestock and poultry feed resource development, and particularly relates to a method for fermenting feed by using seed melon peel and pulp.
Background
Seed melons are produced in northwest areas of China, wherein five provinces of Sinkiang, Gansu, inner Mongolia, Ningxia and Qinghai are main production areas. At present, the seed melon becomes a characteristic industry of Xinjiang, particularly in Tacheng and Aletai areas of northern Xinjiang, and the production area of the seed melon reaches 14 kilohm in 20162The above. Seed melon production is an effective way for farmers to defy poverty and become rich and increase income in poverty areas in Xinjiang at present. Especially in recent years, the national causesThe demand of the inside and outside markets on seed melons is increased, the seed melon planting area has a considerable scale, and the planting area reaches 33.3 kilohm in 20152Above, the planting area of only Xinjiang bouquet seed melons reaches 4.3 kilohm in 20162Xinjiang has become the most important and competitive seed melon production base in China.
With the expansion of the planting range, the problem of post-treatment of agricultural byproducts is followed. The fresh melon is sold only in a small part of the market due to reasons such as seasons, storage and the like, the seed melon is only used for seed extraction processing at present, but the melon seeds only account for 3% -5% of the weight of the fresh melon, and the remaining melon pulp and melon peel which account for more than 95% of the weight of the melon cannot be effectively processed and utilized in time, most of the melon pulp and melon peel are discarded as wastes, so that great resource waste is caused, meanwhile, the ecological balance of a farmland is also destroyed, and plant diseases and insect pests are caused, so that the development and application of resources are urgently needed.
The seed melon has high nutritive value, and analysis of various nutritive components of the seed melon shows that the seed melon has rich nutritive components and fresh taste, the water content of the seed melon is 5.04%, the fat content is 20.27%, the protein content is 21.28%, the water content of the peel and pulp is more than 90%, the peel and pulp contain more calcium, phosphorus, potassium and magnesium, the total sugar content of the peel is 4.52%, and the total sugar content of the pulp is 30.89%. The seed kernel and the melon peel contain more than 13 kinds of amino acids. Therefore, the seed melon peel and pulp are potential feed resources with excellent quality.
At present, most of seed melon products are developed domestically by extracting and utilizing active ingredients in seed melon peels and pulp, and although the melon peels and pulp obtained after seeds are collected from the seed melons are directly used as feed, the seed melons are rapidly rotten and deteriorated, the feeding safety cannot be guaranteed, and the seed melons cannot be effectively used as feed. The method for processing feed raw materials and improving the nutritive value by utilizing biotechnology, particularly the microbial fermentation technology, is a safe and efficient method for developing livestock and poultry feed resources
At present, in the Xinjiang area, seed melon is mainly subjected to seed extraction processing, part of seed melon is used in the fresh food market, deep processing of seed melon peel and pulp is in the initial stage, a large-scale processing industrial chain of seed melon series products is not formed, and the seed melon peel and pulp fermented feed is processed by utilizing a microbial fermentation technology, so that the method is a good opportunity for developing the Xinjiang seed melon series product market.
Disclosure of Invention
The invention aims to provide a seed melon peel and pulp fermented composite feed and a preparation method thereof, aiming at the technical current situation that the seed melon pulp and peel can not be processed and utilized timely and effectively, most of the seed melon pulp and peel can be discarded as wastes, so that the resource waste is caused, the ecological balance of a farmland is damaged, the plant diseases and insect pests are caused, and the seed melon peel and pulp can not be used as the feed effectively due to rapid decay and deterioration. The invention utilizes the microbial fermentation technology to prepare the feed of the seed melon peel and pulp, has rich resources, simple and easy technology, is convenient to popularize, can relieve the contradiction between the development of modern animal husbandry and the insufficient sources of forage grass, and has very wide development prospect.
The invention is realized by the following technical scheme:
the invention specifically provides a seed melon peel and pulp fermented compound feed, which is specifically obtained by adopting the following preparation method:
(1) selecting fresh non-rotten seed melon peel and pulp, crushing the seed melon peel into particles, and pulping the seed melon pulp for later use;
(2) crushing the dried wheat straw and the bean pulp for later use;
(3) preparation of a microbial inoculum: the number of viable bacteria is 106—107Activating the lactobacillus acidophilus, saccharomyces cerevisiae and bacillus subtilis freeze-dried powder, and mixing in proportion to prepare a fermentation microbial inoculum;
(4) inoculating and fermenting, namely mixing the prepared seed melon peel and pulp, the dried wheat straw and the soybean meal according to a certain proportion, scattering a zymophyte agent according to a certain weight ratio, fully stirring and uniformly mixing the zymophyte materials, then filling the mixture into a fermentation bag according to the amount of 30 Kg/bag, and treading down and compacting once at the height of 20cm every time, and sealing; the initial fermentation temperature is lower than 27 ℃, the fermentation temperature is not higher than 41 ℃ during fermentation, and the fermentation can be carried out for 30 days, thus being capable of unsealing and feeding.
In the invention, the seed melon peel is preferably crushed into 1-2mm particles.
In the present invention, it is preferable that the soybean meal is pulverized to 200-300. mu.m.
In the present invention, it is preferable that the dried wheat grass is pulverized to 2 to 3 mm.
In the present invention, the number of viable bacteria is preferably 106—107Activating lactobacillus acidophilus, saccharomyces cerevisiae and bacillus subtilis, and mixing according to the weight ratio of 1:1: 1.
In the invention, preferably, the seed melon peel and pulp, the dry wheat straw and the soybean meal are mixed according to the weight ratio of 7:2: 1.
In the invention, preferably, the seed melon peel and pulp, the dry wheat straw and the soybean meal are mixed according to the weight ratio of 7:2:1, and then the mixture is scattered into the zymophyte agent according to the weight ratio of 2%.
The invention mainly uses seed melon peel and pulp as raw materials, uses dry wheat straw and bean pulp as auxiliary materials, and is inoculated with a fermentation inoculum prepared from lactobacillus acidophilus, saccharomyces cerevisiae and bacillus subtilis on the basis. The principle is as follows: the lactobacillus acidophilus can secrete antibiotic substances and has antagonistic action on pathogenic microorganisms; the saccharomyces cerevisiae has high vitamin and protein contents; active substances such as subtilin, polymyxin, nystatin, gramicidin and the like generated in the growth process of bacillus subtilis thallus have obvious inhibiting effect on pathogenic bacteria or conditional pathogenic bacteria of endogenous infection, can stimulate the growth and development of animal immune organs, activate T, B lymphocytes, improve the level of immunoglobulin and antibody, enhance the cellular immunity and humoral immunity functions and improve the immunity of groups, and finally the bacillus subtilis thallus self-synthesizes enzymes such as alpha-amylase, protease, lipase, cellulase and the like to play a role together with digestive enzymes in an animal body in the digestive tract. Under the action of the three bacteria, the contents of amino acids and trace elements such as potassium, calcium, iron, zinc and the like in the seed melon peel and pulp fermented feed can be obviously increased while the seed melon peel and pulp fermented feed is effectively utilized.
In the invention, the lactobacillus acidophilus, the saccharomyces cerevisiae and the bacillus subtilis are common public well-known strains, and can be purchased and obtained by common technicians in the field through public channels, and the culture conditions and the culture media of the three strains can be obtained by common reports in the field.
By implementing the technical scheme of the invention, the following beneficial effects can be achieved:
(1) the content of 17 amino acids in the seed melon peel and pulp fermented compound feed provided by the invention is averagely improved by 9.1% compared with that of the raw material amino acids, and the seed melon peel and pulp fermented feed provided by the invention is very rich in amino acids.
(2) The seed melon peel and pulp fermented composite feed provided by the invention is added into daily ration according to a proportion of 5% to feed Xinjiang fine wool sheep, the daily gain (g) is 101.8+28.9, the daily feed intake (g) is 1554.8+153, the daily dry matter feed intake (g) is 716.9+79.5, the feed efficiency (%) is 15.3+5.3, and the dry matter feed efficiency (%) is 7.0+2.7, so that the seed melon peel and pulp meets the feeding effect of fermented feed, and the seed melon peel and pulp fermented composite feed has obvious effect and feed utilization rate.
(3) The seed melon peel and pulp fermented composite feed provided by the invention is added into daily ration according to the proportion of 5% to feed Aletan sheep, the daily gain (g) is 121.8+24.9, the daily feed intake is 1948.8+129.5, the daily dry matter feed intake is 986.58+87.2, the feed efficiency is 16.0+5.2, and the dry matter feed efficiency is 8.1+3.5, so that the seed melon peel and pulp fermented composite feed meets the feeding effect of fermented feed and has obvious effect and feed utilization rate.
(4) The seed melon peel and pulp fermented composite feed provided by the invention is added into daily ration in a proportion of 5% to feed Xinjiang brown cattle, the daily gain (g) is 140.2+26.1, the daily feed intake is 2523.6+88.7, the daily dry matter feed intake is 1317.9+109.6, the feed efficiency is 18.0+3.4, and the dry matter feed efficiency is 9.4+4.2, so that the seed melon peel and pulp fermented composite feed meets the feeding effect of fermented feed and has obvious effect and feed utilization rate.
(5) According to response surface tests, the key process parameters of the seed melon peel and pulp fermented compound feed are as follows: the grain size of the seed melon peel is 1-2mm, the grain size of the bean pulp is 200-6—107. Tests prove that the more preferable seed melon peel granularity is 1.5mm, the bean pulp granularity is 250 mu m, and the viable count is 5.5 multiplied by 106. Seed melon peel and pulp fermentation type composite material obtained by optimized processThe feed has more vivid yellow green and strong acid flavor, and the feeding effect is better.
(6) The seed melon peel and pulp fermentation type compound feed is added into daily ration in a proportion of 5% to achieve a remarkable feeding effect; wherein the feed efficiency and the dry matter feed efficiency of Xinjiang fine hair sheep fed by the seed melon skin and pulp fermented compound feed are respectively 1.5 times and 2.2 times of the feed efficiency and the dry matter feed efficiency of Xinjiang fine hair sheep fed by the feed only by the feed; when the seed melon peel and pulp fermented compound feed is added for feeding, the feed efficiency and the dry matter feed efficiency of the Aletan sheep are respectively 1.7 times and 1.9 times of the feed efficiency and the dry matter feed efficiency of the Aletan sheep when only the daily ration is fed; when the seed melon peel and pulp fermented composite feed is added for feeding, the feed efficiency and the dry matter feed efficiency of the Xinjiang brown cattle are respectively 1.6 times and 2.1 times of the feed efficiency and the dry matter feed efficiency of the Xinjiang brown cattle when only the daily ration is fed.
Drawings
Fig. 1 shows a graph of response surface curves of seed melon peel particle size and soybean meal particle size to efficacy scores of seed melon peel and pulp fermented composite feeds.
Fig. 2 is a graph showing the response surface curve of the seed melon peel granularity and viable cell count to the efficacy score of the seed melon peel and pulp fermented compound feed.
Fig. 3 is a graph showing the response surface curve of the soybean meal granularity and viable cell count to the efficacy score of the seed melon peel and pulp fermented compound feed.
Detailed Description
The present invention will be described below by way of examples, but the present invention is not limited to the following examples.
The seed melon peel and pulp, the dry wheat straw, the soybean meal, the lactobacillus acidophilus, the saccharomyces cerevisiae and the bacillus subtilis can be purchased through public channels, and equipment and instruments adopted in the process are common equipment in the field.
All materials, reagents and equipment selected for use in the present invention are well known in the art, but do not limit the practice of the invention, and other reagents and equipment well known in the art may be suitable for use in the practice of the following embodiments of the invention.
The first embodiment is as follows: preparation of seed melon peel and pulp fermentation type compound feed
Selecting fresh non-rotten seed melon peel and pulp, crushing the seed melon peel into particles with the particle size of 1-2mm, and pulping the seed melon pulp for later use; pulverizing dry herba Tritici Aestivi to 2-3mm, and pulverizing soybean meal to 200-; preparation of a microbial inoculum: the number of viable bacteria is 106—107Activating lactobacillus acidophilus, saccharomyces cerevisiae and bacillus subtilis, and mixing according to the weight ratio of 1:1:1 to prepare a fermentation microbial inoculum; and (3) inoculating and fermenting, namely mixing the prepared seed melon peel and pulp, the dried wheat straw and the soybean meal according to the weight ratio of 7:2:1, scattering a fermentation microbial inoculum according to the weight ratio of 2%, fully and uniformly stirring the mixed fermentation raw materials, filling the mixture into a fermentation bag according to the amount of 30 Kg/bag, and compacting and sealing the mixture once at the height of 20 cm. Each group is divided into 3 groups, the initial fermentation temperature is lower than 27 ℃, the fermentation temperature is not higher than 41 ℃, and the fermentation time is 30 days, so that the food can be unsealed and fed.
Detection results of various indexes after fermentation
(1) Sensory evaluation
Table 1: sensory evaluation of seed melon peel and pulp fermented compound feed
Sample (I) Color Flavor (I) and flavor (II)
Control sample Brown yellow Sour taste
Sample No Yellow green Sour flavor
Sample No. 2 Yellow green Sour flavor
Sample No. three Yellow green Sour flavor
(2) Organic acid measurement results
Table 2: organic acid content of seed melon peel and pulp fermentation type compound feed
Figure BDA0001613120570000071
(3) Other nutrient determination
Table 3: seed melon peel and pulp fermentation type composite feed nutrient content
Control Sample 1 Sample 2 Sample 3
Total energy (%) 13.95 15.86 15.99 15.32
Crude protein content (%) 18.77 19.02 19.13 19.08
Determination of pH 4.01 4.40 4.43 4.48
Coarse ash (%) 10.78 11.66 10.98 11.34
Neutral detergent fiber (%) 66.70 66.71 66.68 66.56
Acid detergent fiber (%) 41.78 42.91 41.81 42.88
Hemicellulose (%) 24.72 23.92 23.87 22.91
Cellulose (%) 28.17 27.72 27.72 27.78
(4) Determination of amino acid content in seed melon peel and pulp fermented compound feed
Table 4: seed melon peel and pulp fermentation type compound feed amino acid content (mg/L)
Figure BDA0001613120570000081
Figure BDA0001613120570000091
From table 4, it can be seen that the amino acid content of the seed melon peel and pulp fermented compound feed is significantly increased compared with the amino acid content of the seed melon peel and pulp.
Example two: evaluation of quality efficacy of seed melon peel and pulp fermented compound feed by different preparation process parameters
The evaluation of the efficacy of the seed melon peel and pulp fermented compound feed in the first example is shown in table 5.
Table 5: efficacy scoring criteria (score of 30)
Figure BDA0001613120570000092
Note that the total score is the sum of scores of all indexes
The response surface method optimizes the formula, and the test factors and levels are shown in table 6:
table 6: response surface test factors and horizon table
Figure BDA0001613120570000093
Table 7: response surface test design and results
Figure BDA0001613120570000094
Figure BDA0001613120570000101
Response surface test design and results:
the experimental design analysis was performed according to the Box-Benhnken center combinatorial design principle, and the results are shown in Table 7. Fitting the experimental data of the table 7 by adopting a multivariate fitting method through Design expert8.0.6 to obtain a secondary polynomial regression model of the efficacy score R, the seed melon peel granularity (A), the bean pulp granularity (B) and the viable count (C), wherein the secondary polynomial regression model comprises the following steps:
R=27.00-0.12A+0.13B+0.25C-0.25AC+0.25BC-3.00A2-2.50B2-2.75C2
the interaction of the factors in the response surface analysis is described in detail in figures 1 to 3. The key technological parameters of the seed melon peel and pulp fermented compound feed obtained by the response surface test are as follows: the grain size of the seed melon peel is 1-2mm, the grain size of the bean pulp is 200-6—107. Tests prove that the more preferable seed melon peel granularity is 1.5mm, the bean pulp granularity is 250 mu m, and the viable count is 5.5 multiplied by 106
Example three: feeding effect of seed melon peel and pulp fermentation type compound feed
Based on the seed melon peel and pulp fermentation type compound feed implemented in the example one, the compound feed is added into daily ration in a proportion of 5% to feed sheep. The sheep variety in the experiment is Xinjiang fine wool sheep, the sheep is 120 days old in day, and through a 60-day feeding experiment, the daily gain (g) is 101.8+28.9, the daily feed intake is 1554.8+153, the daily dry matter feed intake is 716.9+79.5, the feed efficiency is 15.3+5.3, and the dry matter feed efficiency is 7.0+ 2.7.
Example four: feeding effect of seed melon peel and pulp fermentation type compound feed
Based on the seed melon peel and pulp fermentation type compound feed implemented in the example one, the compound feed is added into daily ration in a proportion of 5% to feed sheep. The sheep variety in the experiment is an alexan sheep which is 120 days old in day, and through a 60-day feeding experiment, the daily gain (g) is 121.8+24.9, the daily feed intake is 1948.8+129.5, the daily dry matter feed intake is 986.58+87.2, the feed efficiency is 16.0+5.2, and the dry matter feed efficiency is 8.1+ 3.5.
Example five: feeding effect of seed melon peel and pulp fermentation type compound feed
Based on the seed melon peel and pulp fermentation type compound feed implemented in the example one, the compound feed is added into daily ration at a ratio of 5% to feed cattle. The cattle variety in the experiment is Xinjiang brown cattle, the cattle is 120 days old, and through a 60-day feeding experiment, the daily gain (g) is 140.2+26.1, the daily feed intake is 2523.6+88.7, the daily dry matter feed intake is 1317.9+109.6, the feed efficiency is 18.0+3.4, and the dry matter feed efficiency is 9.4+ 4.2.
Example six:
feeding Xinjiang fine wool sheep, Aletai sheep and Xinjiang brown cattle which are 120 days old by day with daily ration only, and feeding for 60 days to observe feeding effect, wherein the test group is group A; feeding 120-day-old Xinjiang fine wool sheep, Aletai sheep and Xinjiang brown cattle with a mixed feed which is prepared by mixing 5% of seed melon peel and pulp fermented compound feed added into daily ration, feeding for 60 days, and observing the feeding effect, wherein the test group is a group B; A. the physiological conditions of Xinjiang fine wool sheep, Aletai sheep and Xinjiang brown cattle in the group B are similar. The observations are as follows:
table 8: feeding effect comparison table
Figure BDA0001613120570000111
Figure BDA0001613120570000121
As can be seen from Table 8, the seed melon peel and pulp fermented compound feed has a remarkable feeding effect when being added into daily ration in a proportion of 5%; the feed efficiency and the dry matter feed efficiency of the Xinjiang fine wool sheep in the group B are respectively 1.5 times and 2.2 times of those of the Xinjiang fine wool sheep in the group A; the feed efficiency and dry matter feed efficiency of group B of Aletan sheep are respectively 1.7 times and 1.9 times of those of group A of Aletan sheep; the feed efficiency and dry matter feed efficiency of the Xinjiang brown cattle in the group B are respectively 1.6 times and 2.1 times of those of the Xinjiang brown cattle in the group A.
By implementing the seed melon peel and pulp fermented composite feed and the preparation method thereof provided by the invention, the seed melon pulp and the melon peel can be processed and utilized timely and effectively, resources are saved, the ecological balance of farmlands is protected, the competitiveness of a Xinjiang seed melon production base is favorably improved, and meanwhile, the safe and efficient seed melon peel and pulp fermented composite feed obtained by utilizing a microbial fermentation technology is favorable for relieving the current animal husbandry development and avoiding an important way of competing for grains by people and livestock.
As described above, the present invention can be preferably implemented, and the above-mentioned embodiments only describe the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various changes and modifications of the technical solution of the present invention made by those skilled in the art without departing from the design spirit of the present invention shall fall within the protection scope defined by the present invention.

Claims (6)

1. The seed melon peel and pulp fermented compound feed is characterized by being prepared by the following steps:
(1) selecting fresh non-rotten seed melon peel and pulp, crushing the seed melon peel into particles, and pulping the seed melon pulp for later use;
(2) crushing the dried wheat straw and the bean pulp for later use;
(3) preparation of a microbial inoculum: the number of viable bacteria is 106—107 Activating the lactobacillus acidophilus, saccharomyces cerevisiae and bacillus subtilis freeze-dried powder, and mixing according to a weight ratio to prepare a fermentation microbial inoculum;
(4) inoculating and fermenting, namely mixing the prepared seed melon peel and pulp, the dried wheat straw and the soybean meal according to the weight ratio, scattering a zymophyte agent according to the weight ratio, fully and uniformly stirring and mixing the fermentation raw materials, then filling the mixture into a fermentation bag according to the amount of 30 Kg/bag, and treading down and compacting once at the height of 20cm every time, and sealing; the initial fermentation temperature is lower than 27 ℃, the fermentation temperature is not higher than 41 ℃ during fermentation, and the fermentation can be carried out for 30 days, thus being capable of unsealing and feeding.
2. The seed melon peel and pulp fermented compound feed as claimed in claim 1, wherein in the preparation method, the seed melon peel is crushed into 1-2mm particles in the step (1).
3. The seed melon peel and pulp fermentation type composite feed as claimed in claim 1, wherein the preparation method comprises the steps of (2) pulverizing the dried wheat straw to 2-3mm and pulverizing the soybean meal to 200-300 μm.
4. The seed melon peel and pulp fermented compound feed as claimed in claim 1, wherein the preparation method of the microbial inoculum in step (3) is characterized in that the viable count of the microbial inoculum is 106—107 The lactobacillus acidophilus, the saccharomyces cerevisiae and the bacillus subtilis are activated and then mixed according to the weight ratio of 1:1:1 to prepare the lactobacillus acidophilus/saccharomyces cerevisiae/bacillus subtilis composite material.
5. The seed melon peel and pulp fermented compound feed as claimed in claim 1, wherein in the preparation method, in the step (4), the seed melon peel and pulp, the dry wheat straw and the soybean meal are mixed according to a weight ratio of 7:2: 1.
6. The seed melon peel and pulp fermented compound feed as claimed in claim 1, wherein in the preparation method, in the step (4), the seed melon peel and pulp, the dry wheat straw and the soybean meal are mixed according to a weight ratio of 7:2:1, and then the fermenting agent is sprinkled according to a weight ratio of 2%.
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CN105454672A (en) * 2015-12-10 2016-04-06 内蒙古农业大学 Preparation method of corn processing by-product fermented feed and used composite microbial agent
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