CN113115869A - Microbial fermentation feed and preparation method thereof - Google Patents
Microbial fermentation feed and preparation method thereof Download PDFInfo
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- CN113115869A CN113115869A CN202010027223.2A CN202010027223A CN113115869A CN 113115869 A CN113115869 A CN 113115869A CN 202010027223 A CN202010027223 A CN 202010027223A CN 113115869 A CN113115869 A CN 113115869A
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
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- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
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Abstract
The invention discloses a microbial fermentation feed, which comprises the following raw materials in parts by weight: 20-60 parts of clay powder, 8-12 parts of plant stem and leaf powder edible for pigs, 20-55 parts of plant protein nutrient substance-containing powder, 5-20 parts of animal protein nutrient substance-containing powder and 0.5-1.5 parts of microbial fermentation strain composition. The invention also provides a preparation method of the microbial fermentation feed. The microbial fermentation feed has the advantages of improving the disease prevention and resistance of pigs, improving pork quality and improving the gastrointestinal environment of pigs, does not need to add any antibiotic, is nutritional and healthy, and the excrement discharged by the pigs after eating the feed is odorless, does not breed mosquitoes and does not pollute the environment. The preparation method of the microbial fermentation feed is simple, has low cost and is convenient for realizing large-scale industrial production.
Description
Technical Field
The invention relates to the field of feeds, in particular to a microbial fermentation feed and a preparation method thereof.
Background
In the existing feed production, natural foods such as corn, bean pulp, bran and the like are used as raw materials, and although the natural foods have good nutritional functions, the natural foods do not have the effects of disease prevention and disease resistance, and in the existing livestock and poultry breeding system, antibiotics are often added into the feed in order to improve the disease resistance and disease prevention capability of livestock and poultry. As is known to all, after livestock and poultry eat feed added with antibiotics, the antibiotics can be enriched in the bodies of the livestock and poultry, and after people eat meat products, the drug resistance of the human body is deteriorated due to the problems of antibiotic residue and the like, so that great harm can be brought to the health of human beings if the drug resistance is long-term.
To solve this problem, substances capable of replacing antibiotics in feed have been sought. Later, with the development of biotechnology, especially microbial fermentation technology, people put research perspectives into the field of microbial fermentation and have invented various microbial fermented feeds:
for example, the chinese patent application publication No. CN102326667A discloses a method for preparing fermented feed for antibiotic-free high viable bacteria livestock and poultry, comprising the following steps: 1) crushing feed base material and strain culture medium to 10-100 meshes, wherein based on 100 percent of the total weight of the feed base material, the feed base material comprises 65-70 percent of corn, 15-20 percent of soybean meal, 5-10 percent of cottonseed meal, 2-10 percent of bran and 1-4 percent of premix; inoculating mixed bacterial liquid with the total bacterial number more than 108cfu/g according to 10-40% of the total weight of the feed base material, and uniformly mixing, wherein the mixed bacterial liquid consists of aerobic bacteria and facultative anaerobic bacteria; 2) the mixed materials are put into a packaging bag with a one-way air outlet valve device for sealed packaging, and the fermented feed for the livestock and poultry without the antibiotic high viable bacteria is prepared after standing and solid state fermentation for more than 5 days; the fermented feed prepared by the method has comprehensive nutrition of the mixture, is fermented, has biological activity, does not remain, and does not generate drug resistance.
For another example, chinese patent application publication No. CN109588538A discloses a method for preparing a microbial fermented feed containing no antibiotics, wherein the fermentation raw materials comprise the following components in parts by weight: 500 parts of rice bran protein powder, 400 parts of dry bean curd residue, 200 parts of waste molasses, 50-100 parts of bean pulp, 10-20 parts of complex enzyme and 3-8 parts of compound lactobacillus leavening agent; comprises the following steps: respectively carrying out amplification culture on strains for fermentation, mixing to obtain a compound lactic acid bacteria starter, filling fermentation raw materials, the compound lactic acid bacteria starter and a compound enzyme into an airtight packaging bag, discharging air, and fermenting to obtain a microbial fermented feed without antibiotics; the strain of the compound lactobacillus leaven comprises lactobacillus plantarum, saccharomyces cerevisiae, bacillus coagulans and pediococcus pentosaceus; the complex enzyme comprises amylase, cellulase, protease and glucose oxidase; the volume ratio of the fermentation raw material to the air sealed in the packaging bag is 8-12: 1.
For another example, the Chinese patent application publication No. CN105746850A discloses a low-temperature fermented feed material, which is characterized in that the feed material is prepared by fermenting feed materials of bacillus pumilus, saccharomyces cerevisiae and lactobacillus acidophilus, the probiotic content in fresh fermented feed materials is 109-11 CFU/g, and the organic acid content after drying is 2-3%, wherein the fermented feed material is fermented at the temperature of 15-20 ℃; the feed raw materials are one or more of soybean meal, corn, rice bran meal, rapeseed meal and DDGS, preferably, the soybean meal: corn: rice bran meal: rapeseed meal: the weight ratio of DDGS is 2-5.5:2-5.5:2-3.5:1-3: 0.5-1.
Above feed prepared by microbial fermentation solves the problem brought by using antibiotics in feed to a certain extent, but the feed can be seen from the documents, the ratio of the adopted nutrient components is very large, and the feed is known by the personnel in the breeding field, the digestion function of livestock and poultry is very poor, the absorption and utilization rate of the livestock and poultry to food is very low, so that huge grain waste is caused on one hand, and on the other hand, the excrement discharged is very smelly due to incomplete digestion of too much organic matters in the intestinal tract, and germs and mosquitoes are very easy to breed, thereby bringing very adverse influence to the ecological environment.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide the microbial fermented feed which can improve the disease prevention and resistance of pigs, improve the pork quality, improve the gastrointestinal environment of the pigs, does not need to add any antibiotic, is nutritional and healthy, and can ensure that excrement discharged by the pigs after eating the feed is odorless, does not breed mosquitoes and pollute the environment, and the invention also provides the preparation method of the microbial fermented feed.
The purpose of the invention is realized by adopting the following technical scheme:
a microbial fermentation feed is prepared from the following raw materials in parts by weight: 20-60 parts of clay powder, 8-12 parts of plant stem and leaf powder edible for pigs, 20-55 parts of plant protein nutrient substance-containing powder, 5-20 parts of animal protein nutrient substance-containing powder and 0.5-1.5 parts of microbial fermentation strain composition.
In the invention, the preferable scheme is that the plant stem and leaf powder edible for pigs is one or a combination of more than two of bamboo grass powder, wormwood powder, pine needle powder, sweet potato leaf powder, lettuce leaf powder, canna powder, banana leaf powder, shepherd's purse powder and plantain powder.
In the present invention, it is preferable that the plant protein-containing nutrient substance powder is one or a combination of two or more selected from peanut powder, corn powder, soybean meal powder, rice bran powder and rapeseed meal powder.
In the present invention, it is preferable that the animal protein-containing nutrient powder is selected from fish meal and/or animal bone meal.
In the invention, the preferable scheme is that the raw materials of the microbial fermentation strain composition comprise the following strains in parts by weight: 12-18 parts of lactic acid bacteria, 15-25 parts of yeast, 3-8 parts of mould, 12-18 parts of bacillus, 8-12 parts of streptococcus, 4-6 parts of photosynthetic bacteria, 8-12 parts of bacteroides and 8-12 parts of trichoderma.
In the invention, the preferable scheme is that the preparation raw materials of the feed comprise the following components in parts by weight: 60 parts of clay powder, 5 parts of broad bamboo grass powder, 2 parts of wormwood powder, 3 parts of pine needle powder, 2 parts of peanut bran, 15 parts of corn flour, 4 parts of fish meal, 5 parts of soybean meal, 3 parts of animal bone meal and 1 part of microbial fermentation strain composition.
In the invention, the microbial fermentation feed also comprises 0.05-0.15 part of salt by weight.
The preparation method of the microbial fermentation feed comprises the following steps: diluting the microbial fermentation strain composition with 10-50 times of water to obtain fermentation strain diluent; then, according to the formula, the edible plant stem and leaf powder of the pig, the plant protein nutrient substance-containing powder, the animal protein nutrient substance-containing powder and the clay powder are measured, then the fermentation strain diluent is added, mixed and stirred evenly, and then the mixture is subjected to anaerobic fermentation for 1.5 to 2.5 hours at the temperature of 60 to 70 ℃ to obtain the feed additive.
In the invention, the preferable scheme is that the preparation of the clay powder comprises the following steps: collecting the collected soil, drying the soil to remove water in the soil, and then crushing the soil into soil powder with the particle size of 30-200.
In the present invention, it is preferable that the soil is soil which is less than 1 m from the surface of the hillside.
Compared with the prior art, the invention has the beneficial effects that: according to the fermentation bacteria composition for the feed, disclosed by the invention, part of substances in soil are converted into protein by performing biological fermentation on the soil, and the protein is combined with rich mineral substances and trace elements contained in the soil to form a natural antibiotic-free, hormone-free and additive-free fermentation feed with rich nutrition; the strain composition can inhibit harmful bacteria in the fermentation process, the gastrointestinal environment can be improved after the pig eats the strain composition, the absorption of the feed is promoted, the feed intake of the pig is reduced, a large amount of grains are saved, and the biological competitive advantage of the strain composition is fully added to the feed absorption, so that excrement discharged after the pig eats the feed does not have odor, mosquitoes and mosquitoes, the environment is not polluted, the content of protein in the pork can be increased, the meat product of the raised pig has good taste and rich nutrition, the pork does not have antibiotics and heavy metal residues, and the health of a human body is facilitated.
Drawings
FIG. 1 is a photograph showing a microorganism-fermented feed.
Detailed Description
The present invention is further described below with reference to specific embodiments, and it should be noted that, without conflict, any combination between the embodiments or technical features described below may form a new embodiment.
A microbial fermentation feed is prepared from the following raw materials in parts by weight: 20-60 parts of clay powder, 8-12 parts of plant stem and leaf powder edible for pigs, 20-55 parts of plant protein nutrient substance-containing powder, 5-20 parts of animal protein nutrient substance-containing powder and 0.5-1.5 parts of microbial fermentation strain composition.
According to the fermentation bacteria composition for the feed, disclosed by the invention, part of substances in soil are converted into protein by performing biological fermentation on the soil, and the protein is combined with rich mineral substances and trace elements contained in the soil to form a natural antibiotic-free, hormone-free and additive-free fermentation feed with rich nutrition; the strain composition can inhibit harmful bacteria in the fermentation process, the gastrointestinal environment can be improved after the pig eats the strain composition, the absorption of the feed is promoted, the feed intake of the pig is reduced, a large amount of grains are saved, and the biological competitive advantage of the strain composition is fully added to the feed absorption, so that excrement discharged after the pig eats the feed does not have odor, mosquitoes and mosquitoes, the environment is not polluted, the content of protein in the pork can be increased, the meat product of the raised pig has good taste and rich nutrition, the pork does not have antibiotics and heavy metal residues, and the health of a human body is facilitated.
According to the invention, the plant stem and leaf powder edible for pigs is obtained by drying corresponding plant stems and leaves to remove water in the plant stems and leaves, and then crushing the plant stems and leaves into the plant stem and leaf powder edible for pigs with the particle size of 30-200, wherein the plant stems and leaves edible for pigs are stems and leaves of various plants which can be used by the existing pigs, and comprise Chinese herbal medicines, vegetables, wild herbs, other plant stems and leaves and the like, such as pleioblastus bambusoides, wormwood, pine needles, sweet potato leaves, lettuce leaves, canna, banana leaves, shepherdspurse herb and plantain herb. In specific use, one or more of the components can be selected and matched.
In the invention, the nutrient substance powder containing the plant protein can be selected from peanut powder, corn powder, soybean meal powder, rice bran powder, rapeseed meal powder and the like which are commonly used in the existing feed, and the powder is obtained by respectively drying and crushing corresponding substances such as peanut, corn, soybean meal, rice bran and the like. In specific use, one or more of the components can be selected and matched.
In the invention, the nutrient powder containing animal protein can be selected from fish meal, animal bone meal and the like which are commonly used in the existing feed. In specific use, one or more of the components can be selected and matched.
In the invention, as for the microbial fermentation strain composition, the raw materials of the microbial fermentation strain composition comprise the following strains in parts by weight: 12-18 parts of lactic acid bacteria, 15-25 parts of yeast, 3-8 parts of mould, 12-18 parts of bacillus, 8-12 parts of streptococcus, 4-6 parts of photosynthetic bacteria, 8-12 parts of bacteroides and 8-12 parts of trichoderma.
In the invention, the lactic acid bacteria can be selected from various lactic acid bacteria used in the fermentation process of feed or food, such as bifidobacterium adolescentis, lactobacillus bulgaricus, streptococcus lactis, lactobacillus plantarum and lactobacillus lactis; in the actual use process, one or more than two combinations can be selected for matching.
In the invention, the mould can be selected from various moulds used in the fermentation process of feed or food, such as aspergillus niger, streptomyces, aspergillus sulfureus and aspergillus oryzae, and one or more than two moulds can be selected to be matched in the actual use process.
In the invention, the bacillus can be selected from various bacillus used in the fermentation process of feed or food, such as bacillus subtilis, bacillus amyloliquefaciens, bacillus lysinate, bacillus pumilus and bacillus licheniformis, and one or more of the bacillus can be selected to be matched in the actual use process.
In the invention, the streptococcus can be selected from various streptococcus used in the process of fermenting feed or food, such as streptococcus thermophilus and streptococcus citruscitrate, and one or more of streptococcus thermophilus and streptococcus citruscitrate can be selected to be matched in the actual use process.
In the invention, the light and bacteria can be selected from various light and bacteria used in the fermentation process of feed or food, such as rhodopseudomonas palustris and rhodobacter sphaeroides, and one or more than two of the light and bacteria can be selected for matching in the actual use process.
In the invention, the bacteroides can be selected from various bacteroides used in the fermentation process of feeds or foods, such as amylophaga bacteroides, bacteroides hirsutus, ruminophagus bacteroides, bacteroides succinogenes and bacteroides fragilis, and one or more of the bacteroides can be selected to be matched in the actual use process.
In the invention, the yeast can be selected from various yeasts used in the process of fermenting feed or food, such as candida utilis, pichia pastoris, saccharomyces boulardii and saccharomyces cerevisiae, and one or more than two of the yeasts can be selected for matching in the actual use process.
Each of the strains of the present invention can be obtained from a commercially available source.
In the invention, 0.05 to 0.15 part of salt by weight can be added into the feed in order to balance the nutrition of the feed.
The preparation method of the microbial fermentation feed comprises the following steps: diluting the microbial fermentation strain composition with 10-50 times of water to obtain fermentation strain diluent; then, according to the formula, the edible plant stem and leaf powder of the pig, the plant protein nutrient substance-containing powder, the animal protein nutrient substance-containing powder and the clay powder are measured, then the fermentation strain diluent is added, mixed and stirred evenly, and then the mixture is subjected to anaerobic fermentation for 1.5 to 2.5 hours at the temperature of 60 to 70 ℃ to obtain the feed additive.
In the invention, the preparation of the clay powder comprises the following steps: collecting the collected soil, drying the soil to remove water in the soil, and then crushing the soil into soil powder with the particle size of 30-200. Selecting soil powder, namely selecting soil with the distance of less than 1 meter from the ground surface; in order to make the feed healthier, the soil is selected from soil which is 1 m away from the hillside ground, the soil is pollution-free, does not contain harmful substances such as antibiotics and pesticide residues, has extremely low heavy metal content, and does not cause damage to the raised pigs.
The feed prepared by the method can be directly eaten by pigs, and can be granulated and dried for convenient storage and transportation, and can be correspondingly bagged, packaged and the like.
Example 1
A microbial fermentation feed is prepared from the following raw materials in parts by weight: 60 parts of clay powder, 5 parts of broad bamboo grass powder, 2 parts of wormwood powder, 3 parts of pine needle powder, 2 parts of peanut bran, 15 parts of corn flour, 4 parts of fish meal, 5 parts of soybean meal, 3 parts of animal bone meal and 1 part of microbial fermentation strain composition.
Example 2
A microbial fermentation feed is prepared from the following raw materials in parts by weight: 20 parts of clay powder, 5 parts of broad bamboo grass powder, 3 parts of wormwood powder, 4 parts of pine needle powder, 2 parts of peanut bran, 45 parts of corn powder, 8 parts of fish meal, 8 parts of soybean meal, 4.5 parts of animal bone powder and 0.5 part of microbial fermentation strain composition.
Example 3
A microbial fermentation feed is prepared from the following raw materials in parts by weight: 40 parts of clay powder, 5 parts of broadleaf bamboo grass powder, 2 parts of wormwood powder, 1 part of pine needle powder, 5 parts of peanut bran, 30 parts of corn flour, 3 parts of fish meal, 10.5 parts of soybean meal, 2 parts of animal bone meal and 1.5 parts of microbial fermentation bacteria composition.
Example 4
A microbial fermentation feed is prepared from the following raw materials in parts by weight: 60 parts of clay powder, 5 parts of broad bamboo grass powder, 2 parts of wormwood powder, 3 parts of pine needle powder, 2 parts of peanut bran, 15 parts of corn flour, 4 parts of fish meal, 5 parts of soybean meal, 3 parts of animal bone meal, 1 part of microbial fermentation strain composition and 0.1 part of salt.
Example 5
A microbial fermentation feed is prepared from the following raw materials in parts by weight: 50 parts of clay powder, 5 parts of broad bamboo grass powder, 2 parts of wormwood powder, 1 part of pine needle powder, 7 parts of peanut bran, 34 parts of corn flour, 5 parts of fish meal, 10.5 parts of soybean meal, 15 parts of animal bone meal and 1.5 parts of microbial fermentation strain composition.
In each of examples 1 to 5, the soil powder was prepared by drying the soil on the slope of a mountain 1 m or less from the ground surface, and pulverizing the soil powder to a particle size of 30 to 200.
In the above examples 1 to 5, the raw materials for preparing the microbial fermentation strain composition include the following strains in parts by weight: 15 parts of lactic acid bacteria, 20 parts of saccharomycetes, 5 parts of mould, 15 parts of bacillus, 10 parts of streptococcus, 5 parts of photosynthetic bacteria, 10 parts of bacteroides and 10 parts of trichoderma.
Preparing a culture medium: dissolving glucose 20g, peptone 20g, yeast extract 10g, and agar 20g with distilled water, diluting to 1000mL, sterilizing at 121 deg.C for 20min-30min, and adjusting pH to 5.0-5.5 to obtain culture medium.
The compositions of examples 1 to 4 were inoculated into the above-mentioned medium in the respective proportions, and anaerobically cultured at 35 ℃ for 24 hours to obtain a microorganism strain composition.
Wherein, the lactobacillus is selected from lactobacillus with the preservation number of CCTCC NO: m2010150 lactobacillus plantarum LP, wherein the yeast is selected from lactobacillus plantarum LP with the preservation number of CGMCC NO: 2.281 and the preservation numbers of candida utilis and mould are CGMCC NO: 7927 Aspergillus niger and Bacillus are selected with the preservation number of CGMCC NO: 4628 the preservation numbers of the bacillus subtilis and the streptococcus are CGMCC No: 7697 Streptococcus thermophilus and photosynthetic bacteria are selected with preservation number of CGMCC NO: 1.5007 Rhodopseudomonas palustris and Bacteroides with preservation number of CGMCC NO: 10685 the bacteroides fragilis and trichoderma with the preservation number of CGMCC NO: 10928 Trichoderma sp.
The present application does not relate to the biological material preservation procedure, and the materials referred to in the examples are all known materials in the art.
The feeds of the above examples 1-5 were all prepared by the following steps: diluting the microbial fermentation strain composition with 10-50 times of water to obtain fermentation strain diluent; then, according to the formula, the edible plant stem and leaf powder of the pig, the plant protein nutrient substance-containing powder, the animal protein nutrient substance-containing powder and the clay powder are measured, then the fermentation strain diluent is added, mixed and stirred evenly, and then the mixture is subjected to anaerobic fermentation for 1.5 to 2.5 hours at the temperature of 60 to 70 ℃ to obtain the feed additive.
Application example-cultivation of pigs
The feed prepared in the embodiment 1-5 is taken respectively, then the pig breeding is carried out in the following mode, the breeding is carried out in the same farm and is divided into five rows, five piglets in each row have weight difference of no more than 500g, and the specific breeding method comprises the following steps:
and (3) a piglet feeding stage: feeding 1.8 jin of feed to each pig every day, and feeding the feed 1 time every day, wherein the temperature of the pigsty is kept between 25 and 30 ℃, and the humidity of the pigsty is kept between 50 and 70 percent; the piglet feed is prepared from the following raw materials in percentage by weight:
a feeding stage in a growth period: feeding 6 jin of feed to each pig every day, and feeding the feed 1 time every day, wherein the temperature of the pigsty is kept between 25 and 30 ℃, and the humidity of the pigsty is kept between 50 and 70 percent; the feed in the growing period is prepared from the following raw materials in percentage by weight:
and (3) feeding stage in fattening period: feeding 10 jin of feed to each pig every day, feeding the feed 1 time every day, keeping the temperature of the pigsty at 25-30 ℃, and keeping the humidity of the pigsty at 50-70%.
In the process of feeding the feed to the pigs, the feed containing soil components stays in the gastrointestinal environment of the pigs for a long time, so that the stay time of the feed in the digestive system of the pigs is prolonged, the absorption efficiency of the pigs on nutrient substances in the feed is greatly improved, the nutrient conversion rate is greatly improved, and the amino acid content and the protein content of the obtained pork are obviously improved compared with the pork quality obtained by traditional breeding.
The feed is adopted for breeding, so that the food intake of pigs is greatly reduced, a large amount of food is further saved, in addition, the excrement discharged by the pigs does not contain excessive ammonia and other substances with odor, the air of the breeding environment is good, and no odor is generated; on the other hand, the feed can create a good probiotic growth environment in the process of passing through a digestive system, so that the pig grows healthily, the immunity, disease prevention and resistance are enhanced, and the meat quality is better; meanwhile, after the pig digests the absorbed biological proteins and other nutrients, certain nutrients still exist in excretion of the pig, and meanwhile, more microbial fermentation strains exist, and can continue to exert biological competitive advantages in excrement and inhibit the growth of other microorganisms and fly eggs, so that breeding of mosquitoes and flies and other harmful bacteria on a breeding site is avoided, the breeding environment of the pig is better, and the meat quality of the fed pig is better.
Pork quality detection
The meat quality of the pork bred in example 1 was examined, and the examination results are shown in tables 1 to 4.
1. Sample state: solid state; sample number: 1759 g; and (3) packaging condition: bagging with plastic; detecting items: crude fat, saturated fatty acid, stearic acid, palmitic acid, unsaturated fatty acid, oleic acid, linolenic acid, linoleic acid, aspartic acid, methionine, threonine, serine, valine, phenylalanine, leucine, tyrosine, lysine, proline, arginine, histidine, glycine, alanine, isoleucine, glutamic acid, amino acids (16 kinds of amino acids total amount); the results of the examination are shown in Table 1.
TABLE 1
2. Sample state: solid state; sample number: 1759 g; and (3) packaging condition: bagging with plastic; detecting items: oxytetracycline, florfenicol, lead, cadmium, total mercury and total arsenic; the results of the examination are shown in Table 2.
TABLE 2
3. Sample state: solid state; sample number: 1759 g; and (3) packaging condition: bagging with plastic; detecting items: energy, protein, carbohydrate, sodium, fat, nutrient profile, moisture, ash; the results of the specific examination are shown in tables 3 to 4.
TABLE 3
Detecting items | Basis of detection | The result of the detection |
(Energy) | GB 28050-2011 | 476kJ/100g |
Protein | First method of GB 5009.5-2016 | 20.6g/100g |
Carbohydrate compound | GB 28050-2011 | Not detected (<0.5g/100g) |
Sodium salt | First method of GB 5009.91-2017 | 27.1mg/100g |
Fat | First method of GB 5009.6-2016 | 3.4g/100g |
Moisture content | First method of GB 5009.3-2016 | 74.3g/100g |
Ash content | First method of GB 5009.4-2016 | 1.2g/100g |
Nutrient composition table | GB 28050-2011 | See Table 4 |
TABLE 4
Detecting items | Each 100 g | Reference value of nutrient |
(Energy) | 476 kilojoule | 6% |
Protein | 20.6 g | 34% |
Fat | 3.4 g | 6% |
Carbohydrate compound | 0g | 0% |
Sodium salt | 27 mg of | 1% |
The inventor also can detect the detection result with the approximate data by detecting the pigs fed by the feed obtained in other embodiments through the method, and the pig fed by the feed has the advantages of good meat quality, high nutritional value, no harmful substances such as antibiotics and the like.
The above embodiments are only preferred embodiments of the present invention, and the protection scope of the present invention is not limited thereby, and any insubstantial changes and substitutions made by those skilled in the art based on the present invention are within the protection scope of the present invention.
Claims (10)
1. The microbial fermentation feed is characterized in that the preparation raw materials of the feed comprise the following components in parts by weight: 20-60 parts of clay powder, 8-12 parts of plant stem and leaf powder edible for pigs, 20-55 parts of plant protein nutrient substance-containing powder, 5-20 parts of animal protein nutrient substance-containing powder and 0.5-1.5 parts of microbial fermentation strain composition.
2. The microbial fermented feed according to claim 1, wherein the plant stem and leaf powder edible for pigs is selected from one or a combination of more than two of bamboo grass powder, wormwood powder, pine needle powder, sweet potato leaf powder, lettuce leaf powder, canna powder, banana leaf powder, shepherd's purse powder and plantain powder.
3. The microbial fermented feed according to claim 1, wherein the plant protein-containing nutrient powder is one or a combination of two or more selected from peanut powder, corn flour, soybean meal, rice bran powder and rapeseed meal.
4. The microbial fermented feed according to claim 1, wherein the animal protein-containing nutrient powder is selected from fish meal and/or animal bone meal.
5. The microbial fermentation feed as claimed in claim 1, wherein the raw materials of the microbial fermentation strain composition comprise the following strains in parts by weight: 12-18 parts of lactic acid bacteria, 15-25 parts of yeast, 3-8 parts of mould, 12-18 parts of bacillus, 8-12 parts of streptococcus, 4-6 parts of photosynthetic bacteria, 8-12 parts of bacteroides and 8-12 parts of trichoderma.
6. The microbial fermented feed according to claim 1, wherein the raw materials for preparing the feed comprise the following components in parts by weight: 60 parts of clay powder, 5 parts of broad bamboo grass powder, 2 parts of wormwood powder, 3 parts of pine needle powder, 2 parts of peanut bran, 15 parts of corn flour, 4 parts of fish meal, 5 parts of soybean meal, 3 parts of animal bone meal and 1 part of microbial fermentation strain composition.
7. The microbially fermented feed according to any one of claims 1 to 6, further comprising 0.05 to 0.15 parts by weight of a salt.
8. The method for producing a microbial fermented feed according to any one of claims 1 to 6, characterized by comprising the steps of: diluting the microbial fermentation strain composition with 10-50 times of water to obtain fermentation strain diluent; then, according to the formula, the edible plant stem and leaf powder of the pig, the plant protein nutrient substance-containing powder, the animal protein nutrient substance-containing powder and the clay powder are measured, then the fermentation strain diluent is added, mixed and stirred evenly, and then the mixture is subjected to anaerobic fermentation for 1.5 to 2.5 hours at the temperature of 60 to 70 ℃ to obtain the feed additive.
9. The method for preparing a microbial fermented feed according to claim 8, wherein the preparation of the soil powder comprises the following steps: collecting the collected soil, drying the soil to remove water in the soil, and then crushing the soil into soil powder with the particle size of 30-200.
10. The method of claim 9, wherein the soil is less than 1 m from the surface of the hillside.
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CN114947010A (en) * | 2022-03-28 | 2022-08-30 | 西南大学 | Microbial silage additive for reed and triarrhena sacchariflora silage |
CN115736093A (en) * | 2022-11-11 | 2023-03-07 | 上海家膳侍生物科技有限公司 | Biological fermentation nutrient solution, preparation method thereof and application thereof in animal feed |
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