CN103749967B - Sweet potato dregs fermented feed and preparation method thereof - Google Patents

Sweet potato dregs fermented feed and preparation method thereof Download PDF

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Publication number
CN103749967B
CN103749967B CN201410039421.5A CN201410039421A CN103749967B CN 103749967 B CN103749967 B CN 103749967B CN 201410039421 A CN201410039421 A CN 201410039421A CN 103749967 B CN103749967 B CN 103749967B
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bacterium liquid
potato dregs
fermentation
bacterial classification
bacillus subtilis
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CN201410039421.5A
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Chinese (zh)
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CN103749967A (en
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于会民
陈宝江
孙展英
程书梅
刘树栋
安文亭
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中国农业科学院饲料研究所
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Abstract

The invention discloses a kind of sweet potato dregs fermented feed and preparation method thereof, relate to from the obtained field of animal feed of vegetable material.With potato dregs 75-80% and wheat bran 20-25% for supplementary material, this supplementary material basis adds reagent: urea 0.75-1.5%, ammonium sulfate 0.75-1.5%, potassium dihydrogen phosphate 0.5-0.7% and magnesium sulfate 0.4-0.6%, be mixed to get fermentation substrate, be mixed into combined fermentation bacterial classification bacterium liquid with the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum, aerobic fermentation is prepared from; The weight ratio of fermentation substrate and combined fermentation bacterial classification bacterium liquid is 1:1-1.2; Four bacteria liquids are long-pending than being 1-3:1-3:1-3:1-3.The present invention can increase the true protein content of fermentation substrate, reduces crude fibre amount, improves feed palatability and digestive utilization ratio, improves sweet potato dregs nutritive value comprehensively, have economy and environment double benefit.

Description

Sweet potato dregs fermented feed and preparation method thereof
Technical field
The present invention relates to from the obtained technical field of animal of vegetable material.
Background technology
China is sweet potato producing country maximum in the world, and long-term sweet potato cultivated area is 7500 ~ 8,000 ten thousand mu, and account for 4.2% of China total cultivated area, account for the cultivated area in the world about 60%, output accounts for the world and always produces about 80%.Sweet potato dregs is the residue produced in sweet potato starch process, its water content is high, it is many certainly to carry disease germs, easily putrid and deteriorated, in dry, protein content is low, fiber content is high, usually be taken as discarded object abandon or simply utilized as low-quality feedstuff, cause the waste of resource and the pollution of environment, how development and utilization sweet potato dregs resource has become current China starch industry difficult problem in the urgent need to address.By biotechnology, particularly microbial fermentation technology process feedstuff, raising nutritive value, be the safe and efficient method of low-quality feed resource exploitation.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of sweet potato dregs fermented feed and preparation method thereof, the true protein content of fermentation substrate can be increased, reduce crude fibre amount, improve feed palatability and digestive utilization ratio, improve sweet potato dregs nutritive value comprehensively, there is economy and environment double benefit.
For solving the problems of the technologies described above, the technical solution used in the present invention is: a kind of sweet potato dregs fermented feed, with potato dregs 75-80wt% and wheat bran 20-25wt% for supplementary material, this supplementary material basis adds reagent: urea 0.75-1.5wt%, ammonium sulfate 0.75-1.5wt%, potassium dihydrogen phosphate 0.5-0.7wt% and magnesium sulfate 0.4-0.6wt%, be mixed to get fermentation substrate, be mixed into combined fermentation bacterial classification bacterium liquid with the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum, aerobic fermentation is prepared from; Water content≤the 14wt% of described potato dregs; The weight ratio of fermentation substrate and combined fermentation bacterial classification bacterium liquid is 1:1-1.2; The bacterial concentration of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum is 1 × 10 6individual/ml, combined fermentation bacterial classification bacterium liquid is mixed according to the ratio that volume ratio is 1-3:1-3:1-3:1-3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum.
Preferred combined fermentation bacterial classification bacterium liquid is mixed according to the ratio that volume ratio is 2:2:3:1 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum.
The preparation method of sweet potato dregs fermented feed, comprises the following steps:
(1) with potato dregs 75-80wt% and wheat bran 20-25wt% for supplementary material, this supplementary material basis adds reagent: urea 0.75-1.5wt%, ammonium sulfate 0.75-1.5wt%, potassium dihydrogen phosphate 0.5-0.7wt% and magnesium sulfate 0.4-0.6wt%, mix, make fermentation substrate; Water content≤the 14wt% of described potato dregs.
(2) above-mentioned fermentation substrate is mixed into combined fermentation bacterial classification bacterium liquid with the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum, fermentation substrate and combined fermentation bacterial classification bacterium liquid press the weight ratio Homogeneous phase mixing of 1:1-1.2,28-30 DEG C of aerobic fermentation 48-72 hour, dries and pulverizes; The bacterial concentration of described bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum is 1 × 10 6individual/ml, combined fermentation bacterial classification bacterium liquid is mixed according to the ratio that volume ratio is 1-3:1-3:1-3:1-3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum.
Preferred combined fermentation bacterial classification bacterium liquid is mixed according to the ratio that volume ratio is 2:2:3:1 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum.
Bacillus subtilis (Bacillussubtilis) 10075, is purchased from Chinese industrial Microbiological Culture Collection administrative center; Saccharomycopsis fibuligera (Endomycopsisfibuligera) 20154, Trichoderma viride (Trichodermaviride) 30498, Lactobacillus plantarum (Lactobacillusplantarum) 11016, be purchased from Chinese agriculture Microbiological Culture Collection administrative center.
The preparation of bacillus subtilis used, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum bacterium liquid can adopt prior art, the invention provides following methods.
1, the preparation of Lactobacillus plantarum bacterium liquid
The Lactobacillus plantarum that 4 DEG C of inclined-planes are preserved is connected on MRS liquid tube culture medium and activates, with 220rmin at 37 DEG C -1shaken cultivation 24h, cultivates seed as one-level; Be connected in MRS triangular flask fluid nutrient medium, with 220rmin at 37 DEG C with 1% inoculum concentration again -1shaken cultivation 24h.MRS culture medium: peptone 10g, beef extract 10g, yeast extract 5g, hydrogen citrate 2g, glucose 20g, Tween 80 1ml, sodium acetate 5g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.58g, manganese sulfate 0.25g, distilled water 1000ml, pH6.2 ~ 6.6,121 DEG C moist heat sterilization 15min.
2, the preparation of saccharomycopsis fibuligera bacterium bacterium liquid
The saccharomycopsis fibuligera bacterium that 4 DEG C of inclined-planes are preserved is connected on YEPD liquid tube culture medium and activates, with 220rmin at 30 DEG C -1shaken cultivation 24h, cultivates seed as one-level; Be connected in YEPD triangular flask fluid nutrient medium, with 220rmin at 30 DEG C with 1% inoculum concentration again -1shaken cultivation 24h.YEPD culture medium: dusty yeast 10g, peptone 20g, glucose 20g, distilled water 1000ml, pH6.0,115 DEG C of moist heat sterilization 20min.
3, the preparation of bacillus subtilis bacterium liquid
The bacillus subtilis that 4 DEG C of inclined-planes are preserved is connected on beef extract-peptone liquid tube culture medium and activates, with 220rmin at 32 DEG C -1shaken cultivation cultivates 24h, cultivates seed as one-level; Be connected in beef extract-peptone triangular flask fluid nutrient medium, with 220rmin at 32 DEG C with 1% inoculum concentration again -1shaken cultivation 24h.Beef-protein medium: beef extract 0.5g, peptone 15g, sodium chloride 5g, glucose 20g, distilled water 1000ml, pH7.0 ~ 7.2,115 DEG C moist heat sterilization 20min.
4, the preparation of trichoderma viride liquid
The Trichoderma viride that 4 DEG C of inclined-planes are preserved is connected on Cha Shi liquid tube culture medium and activates, with 220rmin at 28 DEG C -1shaken cultivation cultivates 36h, cultivates seed as one-level; Be connected in Cha Shi triangular flask fluid nutrient medium, with 220rmin at 28 DEG C with 1% inoculum concentration again -1shaken cultivation 36h.Czapek's medium: sodium nitrate 3g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.5g, potassium chloride 0.5g, ferrous sulfate 0.01g, sucrose 30g, distilled water 1000ml, pH nature, 121 DEG C of moist heat sterilization 20min.
It is raw material that the present invention mainly applies potato dregs, wheat bran is auxiliary material, add reagent on this basis: urea, ammonium sulfate, potassium dihydrogen phosphate and magnesium sulfate, be mixed to get fermentation substrate, through the combined fermentation bacterial classification bacterium liquid that the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum bacterium liquid is mixed into, aerobic fermentation is prepared from.Its principle is: potato dregs and wheat bran contain enough starch and crude fibre and a small amount of true albumen; Urea, ammonium sulfate contain the available a large amount of inorganic nitrogen of microorganism; Magnesium sulfate, potassium dihydrogen phosphate can promote that some beneficial microbe grows; Above-mentioned three class material action compensatings, application simultaneously can produce Overlay.
Application saccharomycopsis fibuligera can improve fermentation substrate true protein content; Bacillus subtilis can produce protease, improves protein utilization rate; Trichoderma viride can eccrine fiber element enzyme degradation of fibers; Lactobacillus plantarum can produce lactic acid, improves feed local flavor; Fermentation substrate is after aerobic fermentation process, and true protein content is 17.61%, and total amount improves 44.30%, and crude fiber content is 26.60%, and total amount reduces 26.60%, and has sweet-smelling smell.
The beneficial effect adopting technique scheme to produce is:
(1) the present invention can increase the true protein content of fermentation substrate, reduces crude fibre amount, improves feed palatability and digestive utilization ratio, improves sweet potato dregs nutritive value comprehensively, have economy and environment double benefit; Application the inventive method is by after fermentation substrate process, and true protein content is 17.61%, and total amount improves 44.30%, and crude fiber content is 26.60%, and total amount reduces 26.60%, and has sweet-smelling smell.
(2) product of the present invention substitutes wheat bran by 15% part by weight, can improve growing and fattening pig feed intake, daily gain, reduces feedstuff-meat ratio.
(3) the present invention is pure green product, without any side effects, does not produce incompatibility with other raw materials.
Detailed description of the invention
Potato dregs is purchased from Qinhuangdao Gao Cheng food industries Co., Ltd, and water content≤14wt%, market is on sale.
Embodiment 1
Adopt supplementary material and the reagent of following weight composition: potato dregs 160 grams, wheat bran 40 grams, 3 grams, urea, 3 grams, ammonium sulfate, potassium dihydrogen phosphate 1.2 grams, 1 gram, magnesium sulfate.
Preparation method: after above-mentioned supplementary material and reagent are weighed, input pulverizer is pulverized, drop into mixer again to mix, make fermentation substrate, then with the bacterium liquid of bacillus subtilis, button capsule yeast, Trichoderma viride and Lactobacillus plantarum for combined fermentation bacterial classification bacterium liquid, wherein the bacterial concentration of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum is 1 × 10 6individual/ml, combined fermentation bacterial classification bacterium liquid is mixed according to the ratio that volume ratio is 2:2:3:1 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum, fermentation substrate and combined fermentation bacterial classification bacterium liquid press the weight ratio Homogeneous phase mixing of 1:1,28-30 DEG C of aerobic fermentation 48 hours, dry to water content≤14wt% for 70-80 DEG C, pulverize.
Embodiment 2
Adopt supplementary material and the reagent of following weight composition: potato dregs 160 grams, wheat bran 40 grams, 3 grams, urea, 1.5 grams, ammonium sulfate, potassium dihydrogen phosphate 1.2 grams, 1 gram, magnesium sulfate.
Preparation method: be that combined fermentation bacterial classification bacterium liquid is mixed according to the ratio that volume ratio is 1:1:1:1 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum with the difference of embodiment 1; Fermentation substrate and combined fermentation bacterial classification bacterium liquid press the weight ratio Homogeneous phase mixing of 1:1.2.
Embodiment 3
Adopt supplementary material and the reagent of following weight composition: potato dregs 150 grams, wheat bran 50 grams, 3 grams, urea, 3 grams, ammonium sulfate, potassium dihydrogen phosphate 1.2 grams, 1 gram, magnesium sulfate.
Preparation method: be that combined fermentation bacterial classification bacterium liquid is mixed according to the ratio that volume ratio is 1:2:2:2 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum with the difference of embodiment 1; Fermentation substrate and combined fermentation bacterial classification bacterium liquid press the weight ratio Homogeneous phase mixing of 1:1.1.
Embodiment 4
Adopt supplementary material and the reagent of following weight composition: potato dregs 150 grams, wheat bran 50 grams, 3 grams, urea, 1.5 grams, ammonium sulfate, potassium dihydrogen phosphate 1.2 grams, 1 gram, magnesium sulfate.
Preparation method: be that combined fermentation bacterial classification bacterium liquid is mixed according to the ratio that volume ratio is 1:3:3:3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum with the difference of embodiment 1; Fermentation substrate and combined fermentation bacterial classification bacterium liquid press the weight ratio Homogeneous phase mixing of 1:1.05.
Embodiment 5
Adopt supplementary material and the reagent of following weight composition: potato dregs 160 grams, wheat bran 40 grams, 1.5 grams, urea, 1.5 grams, ammonium sulfate, potassium dihydrogen phosphate 1.2 grams, 1 gram, magnesium sulfate.
Preparation method: be that combined fermentation bacterial classification bacterium liquid is mixed according to the ratio that volume ratio is 2:1:2:3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum with the difference of embodiment 1; Fermentation substrate and combined fermentation bacterial classification bacterium liquid press the weight ratio Homogeneous phase mixing of 1:1.15.
Embodiment 6
Adopt supplementary material and the reagent of following weight composition: potato dregs 150 grams, wheat bran 50 grams, 1.5 grams, urea, 1.5 grams, ammonium sulfate, potassium dihydrogen phosphate 1.2 grams, 1 gram, magnesium sulfate.
Preparation method: be that combined fermentation bacterial classification bacterium liquid is mixed according to the ratio that volume ratio is 2:2:3:2 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum with the difference of embodiment 1.
Embodiment 7
Adopt supplementary material and the reagent of following weight composition: sweet potato dregs 156 grams, wheat bran 44 grams, 2 grams, urea, 2.5 grams, ammonium sulfate, potassium dihydrogen phosphate 1 gram, 1.2 grams, magnesium sulfate.
Preparation method: be that combined fermentation bacterial classification bacterium liquid is mixed according to the ratio that volume ratio is 2:3:1:1 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum with the difference of embodiment 1.
Embodiment 8
Adopt supplementary material and the reagent of following weight composition: sweet potato dregs 152 grams, wheat bran 48 grams, 2.5 grams, urea, 2 grams, ammonium sulfate, potassium dihydrogen phosphate 1.4 grams, 0.8 gram, magnesium sulfate.
Preparation method: combine fermented bacterium bacterium liquid with the difference of embodiment 1 and mixed according to the ratio that volume ratio is 3:1:3:2 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum; Fermentation substrate and combined fermentation bacterial classification bacterium liquid press the weight ratio Homogeneous phase mixing of 1:1.2.
Embodiment 9
Adopt supplementary material and the reagent of following weight composition: sweet potato dregs 154 grams, wheat bran 46 grams, 1.7 grams, urea, 1.8 grams, ammonium sulfate, potassium dihydrogen phosphate 1.3 grams, 0.9 gram, magnesium sulfate.
Preparation method: combine fermented bacterium bacterium liquid with the difference of embodiment 1 and mixed according to the ratio that volume ratio is 3:2:1:3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum; Fermentation substrate and combined fermentation bacterial classification bacterium liquid press the weight ratio Homogeneous phase mixing of 1:1.1,28-30 DEG C of aerobic fermentation 72 hours.
Embodiment 10
Adopt supplementary material and the reagent of following weight composition: sweet potato dregs 158 grams, wheat bran 42 grams, 2.2 grams, urea, 2.7 grams, ammonium sulfate, potassium dihydrogen phosphate 1.1 grams, 1.1 grams, magnesium sulfate.
Preparation method: combine fermented bacterium bacterium liquid with the difference of embodiment 1 and mixed according to the ratio that volume ratio is 3:3:2:1 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum, 28-30 DEG C of aerobic fermentation 60 hours.
Its using method of sweet potato dregs fermented feed of the present invention is: substitute the wheat bran of 15% at growing-finishing pigs diets moderate with this product, other raw materials are constant.
Experimental example:
This sweet potato dregs fermented feed affects experimental design to growing-finishing pig production performance
1.1 experimental animals and grouping
Select age in days to differ and be no more than 7 days, about 30 kilograms pigs 120 that kind is identical, identical according to genetic background, that body weight is close, sex ratio is consistent principle is divided into 2 process at random, often 6 repetitions are established in process, and often repeat 10, between each group, starting weight difference is not remarkable.
Control group: basal diet of feeding; Test group: feed and substitute the test daily ration of equivalent wheat bran with 15% sweet potato dregs fermented feed.
1.2 test daily ration and Treatment Design
With reference to NRC(1998) growing-finishing pig nutrition requirements in conjunction with production practices preparation basal diet.
Table 1 Diet Formula and trophic level
1.3 feeding and management
Test is carried out in same confined swine housing, raises on the net, and humiture is suitable for; Free choice feeding and drinking-water.Immunity disinfectant program is undertaken by pig farm conventional method.Test is responsible for by special messenger, manages in strict accordance with test requirements document.Feeding and management is carried out with reference to the feeding and management code of this product boar.Test period 40 days.
1.4. test method
On-test and at the end of in units of individuality, empty stomach is carried out to all test pig and weighs, record body weight in detail; In units of repeating, feed intake is recorded in detail in process of the test; Detailed record pig death and superseded situation, cut in time when there is dead naughty pig and expect and weigh.
1.5 Testing index
The average daily gain of growth performance index, average daily ingestion amount and feedstuff-meat ratio.
1.6 data processings and statistical analysis technique
ANOVA in data acquisition SPSS13.0 statistical software carries out variance analysis; Pat everywhere and carry out the significance of difference by Duncan method between mean and compare, result of the test data represent with mean+SD.
2. results and analysis
Table 2 is on the impact of growing-finishing pig production performance
note: not significantly (P>0.05), the different person of colleague's shoulder mark represents significant difference (P<0.05) to the identical person's difference of colleague's shoulder mark lowercase.
As can be seen from Table 2, test group off-test weight ratio control group improves 2.62%(P>0.05); Average daily gain improves 5.60%(P>0.05 than control group); Average daily ingestion amount improves 0.55%(P>0.05 than control group); Feedstuff-meat ratio reduces 4.85%(P>0.05 than control group), although difference is not remarkable, be all improved trend.
3. conclusion
Can be obtained to draw a conclusion by result of the test:
With sweet potato dregs fermented feed equivalent substitution wheat bran in growing and fattening pig feed, growing and fattening pig feed intake, daily gain can be improved, reduce feedstuff-meat ratio;
Because this product is safe green feedstuff, and cost is significantly lower than substitute-wheat bran, and therefore produce market has a extensive future.

Claims (4)

1. a sweet potato dregs fermented feed, it is characterized in that: with potato dregs 75-80wt% and wheat bran 20-25wt% for supplementary material, this supplementary material basis adds reagent: urea 0.75-1.5wt%, ammonium sulfate 0.75-1.5wt%, potassium dihydrogen phosphate 0.5-0.7wt% and magnesium sulfate 0.4-0.6wt%, be mixed to get fermentation substrate, combined fermentation bacterial classification bacterium liquid is mixed into the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum, 28-30 DEG C of aerobic fermentation 48-72 hour, dries pulverizing and is prepared from; Water content≤the 14wt% of described potato dregs; The weight ratio of fermentation substrate and combined fermentation bacterial classification bacterium liquid is 1:1-1.2; The bacterial concentration of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum is 1 × 10 6individual/ml, combined fermentation bacterial classification bacterium liquid is mixed according to the ratio that volume ratio is 1-3:1-3:1-3:1-3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum.
2. sweet potato dregs fermented feed according to claim 1, is characterized in that described combined fermentation bacterial classification bacterium liquid is mixed according to the ratio that volume ratio is 2:2:3:1 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum.
3. a preparation method for sweet potato dregs fermented feed as claimed in claim 1, is characterized in that, comprise the following steps:
(1) with potato dregs 75-80wt% and wheat bran 20-25wt% for supplementary material, this supplementary material basis adds reagent: urea 0.75-1.5wt%, ammonium sulfate 0.75-1.5wt%, potassium dihydrogen phosphate 0.5-0.7wt% and magnesium sulfate 0.4-0.6wt%, mix, make fermentation substrate; Water content≤the 14wt% of described potato dregs;
(2) above-mentioned fermentation substrate is mixed into combined fermentation bacterial classification bacterium liquid with the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum, fermentation substrate and combined fermentation bacterial classification bacterium liquid press the weight ratio Homogeneous phase mixing of 1:1-1.2,28-30 DEG C of aerobic fermentation 48-72 hour, dries and pulverizes; The bacterial concentration of described bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum is 1 × 10 6individual/ml, combined fermentation bacterial classification bacterium liquid is mixed according to the ratio that volume ratio is 1-3:1-3:1-3:1-3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum.
4. the preparation method of sweet potato dregs fermented feed according to claim 3, is characterized in that described combined fermentation bacterial classification bacterium liquid is mixed according to the ratio that volume ratio is 2:2:3:1 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum.
CN201410039421.5A 2014-01-27 2014-01-27 Sweet potato dregs fermented feed and preparation method thereof CN103749967B (en)

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CN104366116A (en) * 2014-11-26 2015-02-25 邱君 Pig feed
CN104431338A (en) * 2014-12-31 2015-03-25 山东圣琪生物有限公司 Sweet potato dreg type fermented feed and production method thereof
CN105614068B (en) * 2015-12-25 2019-08-20 中国农业大学 A kind of sweet potato beverage residue protein feed and preparation method thereof
CN106173205B (en) * 2016-07-27 2019-12-03 浙江农林大学 A kind of fermented type sweet potato dregs protein feed and the sheep cultural method using its progress
CN106173377B (en) * 2016-07-27 2019-12-03 浙江农林大学 A kind of sweet potato residue fermented feed of ruminant and its application
CN106804875A (en) * 2017-01-19 2017-06-09 淮阴师范学院 A kind of sweet potato residue fermented feed and preparation method and application
CN106942470A (en) * 2017-03-22 2017-07-14 南京致润生物科技有限公司 Biological fermentation feed produced using agricultural wastes and its preparation method and application
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CN103371271A (en) * 2012-04-30 2013-10-30 罗福仲 Processing method for biological fermentation feed auxiliary material with sweet potato peel slag
CN102696875A (en) * 2012-06-26 2012-10-03 广西九通王环保生物工程有限公司 Method for preparing biological feed by taking manioc residues as raw materials
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