CN111990340A - Method for treating biogas residues by using hermetia illucens - Google Patents
Method for treating biogas residues by using hermetia illucens Download PDFInfo
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- CN111990340A CN111990340A CN202010855927.9A CN202010855927A CN111990340A CN 111990340 A CN111990340 A CN 111990340A CN 202010855927 A CN202010855927 A CN 202010855927A CN 111990340 A CN111990340 A CN 111990340A
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- hermetia illucens
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- larvae
- biogas
- residue
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- 241000709785 Hermetia illucens Species 0.000 title claims abstract description 54
- 238000000034 method Methods 0.000 title claims abstract description 22
- 239000007788 liquid Substances 0.000 claims abstract description 19
- 239000000463 material Substances 0.000 claims abstract description 17
- 230000001580 bacterial effect Effects 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 238000009395 breeding Methods 0.000 claims description 15
- 230000001488 breeding effect Effects 0.000 claims description 15
- 239000000203 mixture Substances 0.000 claims description 15
- 210000001835 viscera Anatomy 0.000 claims description 9
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 8
- 241001465754 Metazoa Species 0.000 claims description 7
- 238000000855 fermentation Methods 0.000 claims description 3
- 235000012055 fruits and vegetables Nutrition 0.000 claims description 3
- 210000003608 fece Anatomy 0.000 claims description 2
- 239000010806 kitchen waste Substances 0.000 claims description 2
- 239000010871 livestock manure Substances 0.000 claims description 2
- 244000144977 poultry Species 0.000 claims description 2
- 239000010902 straw Substances 0.000 claims description 2
- 239000002699 waste material Substances 0.000 claims description 2
- 241001481656 Stratiomyidae Species 0.000 claims 1
- 229910001385 heavy metal Inorganic materials 0.000 abstract description 3
- 239000003242 anti bacterial agent Substances 0.000 abstract description 2
- 229940088710 antibiotic agent Drugs 0.000 abstract description 2
- 241000411851 herbal medicine Species 0.000 description 14
- 238000002156 mixing Methods 0.000 description 8
- 241000219112 Cucumis Species 0.000 description 7
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 7
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 7
- 235000013399 edible fruits Nutrition 0.000 description 5
- 244000005700 microbiome Species 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- 235000013311 vegetables Nutrition 0.000 description 5
- 238000005303 weighing Methods 0.000 description 5
- 241000709334 Hermetia Species 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 235000000346 sugar Nutrition 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 235000019750 Crude protein Nutrition 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 235000021120 animal protein Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000003750 conditioning effect Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 238000003900 soil pollution Methods 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
- C02F11/04—Anaerobic treatment; Production of methane by such processes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Health & Medical Sciences (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Animal Behavior & Ethology (AREA)
- General Chemical & Material Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Biodiversity & Conservation Biology (AREA)
- Molecular Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Organic Chemistry (AREA)
- Fodder In General (AREA)
Abstract
The invention provides a method for treating biogas residues by using hermetia illucens, which comprises the steps of adding crushed feeding auxiliary materials and EM bacterial liquid into the biogas residues to prepare mixed feed with the water content of 75-80%, using the mixed feed for feeding 4-day-old hermetia illucens larvae until the larvae enter a prepupation stage, and separating worm residues to obtain treated biogas residues, wherein the treated biogas residues have lower heavy metals and antibiotics and are more environment-friendly.
Description
Technical Field
The invention relates to the technical field of environmental protection, in particular to a method for treating biogas residues by using hermetia illucens.
Background
The biogas residue is a semisolid substance remained at the bottom of the fermentation tank after anaerobic digestion and a solid substance formed after biogas slurry dehydration, and contains rich organic matters, crude protein, nitrogen, phosphorus, potassium and various trace elements. At present, biogas residues are mostly used for preparing organic fertilizers, but certain limitations exist in the using process: excessive use of biogas residues can cause soil pollution and influence the normal growth of plants, and meanwhile, excessive phosphorus in the biogas residues can cause water eutrophication; the use of various additives can cause the hidden danger of pollution to the ecological system of the farmland due to the overhigh content of heavy metal and antibiotics in the biogas residues.
Therefore, a method for performing more environment-friendly treatment on the biogas residues is needed.
Disclosure of Invention
In view of the above, the invention provides a method for green treatment of biogas residues by using hermetia illucens, which has the advantages of simple process, high efficiency, low energy consumption, high additional value and easy large-scale popularization.
The technical scheme of the invention is realized as follows: the invention provides a method for treating biogas residues by using hermetia illucens, which comprises the following steps:
adding crushed feeding auxiliary materials and EM bacterial liquid into biogas residues to prepare mixed feed;
step two, adding the mixed feed obtained in the step one into a black soldier fly breeding pond, breeding black soldier fly larvae until the larvae enter a prepupa stage, and obtaining a residue mixture;
and step three, separating the worm residue mixture obtained in the step two to obtain hermetia illucens larvae and feed residues.
On the basis of the technical scheme, preferably, in the step one, the feeding auxiliary materials are one or a mixture of more of animal internal organs, Chinese herbal medicine dregs, mushroom dregs and melon, fruit and vegetable skins.
On the basis of the above technical scheme, preferably, in the step one, the particle size of the crushed feeding auxiliary material is 60-100 meshes.
On the basis of the technical scheme, preferably, the biogas residues are obtained by performing anaerobic fermentation on one or a mixture of more of kitchen waste, wet waste, poultry manure and straws.
Still more preferably, the water content of the mixed feed obtained in the first step is 75-80%.
On the basis of the technical scheme, preferably, in the step one, the dry basis mass ratio of the feeding auxiliary materials to the biogas residues is 1: (1-1.5).
On the basis of the technical scheme, preferably, the black soldier fly larvae bred in the step two are larvae of 4 days old.
On the basis of the technical scheme, preferably, in the second step, the temperature for breeding the hermetia illucens larvae is 30-35 ℃, and the relative humidity of air is 70-85%.
The preparation method of the EM bacterial liquid comprises the following steps: according to EM solid original strain: brown sugar: uniformly mixing water in a ratio of 10:100:1000, sealing, fermenting at 35-37 ℃, shaking once every 1-2 days, fermenting for 5-7 days to obtain EM liquid strain, mixing the EM liquid strain: brown sugar: mixing water at a ratio of 1:1:10, sealing, maintaining the temperature at 35-37 deg.C, shaking once every 1-2 days, and fermenting for 5-7 days to obtain EM bacterial liquid.
Compared with the prior art, the method for treating biogas residues by using hermetia illucens has the following beneficial effects:
(1) the biogas residues are rich in protein nutrition, but the nutritional ingredients such as oil, vitamins, dietary fibers and sugar are low, the crushed feeding auxiliary materials and the biogas residues can be mixed to play a good conditioning role, so that the moisture, carbon-nitrogen ratio, porosity and the like in the biogas residues are all adjusted, the requirement of high-quality growth of hermetia illucens can be met, the hermetia illucens is utilized to realize rapid conversion and utilization, the biogas residues and the feeding auxiliary materials are converted into animal protein feed, the biogas residues are better recycled and treated, and the treated biogas residues are more environment-friendly;
(2) meanwhile, the EM bacterial liquid is added in the treatment process, so that the utilization rate of the biogas residues can be effectively improved, the black soldier fly larvae can obtain more nutrition, and the EM bacterial liquid can also eliminate the pollution of the biogas residues, so that the black soldier fly larvae is more environment-friendly.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
Example 1
Animal internal organs and Chinese herbal medicine dregs are used as feeding auxiliary materials
Respectively weighing 50 parts of fish viscera and 50 parts of Chinese herbal medicine dregs, respectively crushing, crushing to 60 meshes, adding the crushed fish viscera and the Chinese herbal medicine dregs into 100 parts of biogas dreg dry base, adding EM (effective microorganisms) bacterial liquid, mixing and stirring uniformly to obtain mixed feed, adjusting the adding amount of the EM bacterial liquid to enable the water content of the mixed feed to be 75%, adding the obtained mixed feed into a culture pond of the hermetia illucens to culture the hermetia illucens larvae, putting 4-day-old hermetia illucens larvae into the culture pond, adding 0.02kg of 4-day-old hermetia larvae into each kg of the mixed feed, feeding the mixture at the temperature of 30-35 ℃ and the relative humidity of 70-85%, obtaining a residue mixture when the larvae enter a prepupation stage, and separating the residue to obtain the hermetia illucens larvae and the feed residue.
Example 2
Melon, fruit, vegetable and skin and Chinese herbal medicine dregs are used as feeding auxiliary materials
Respectively weighing 50 parts of melon and fruit vegetable skins and 50 parts of Chinese herbal medicine dregs, respectively crushing the melon and fruit vegetable skins and the Chinese herbal medicine dregs into 100 meshes, adding the crushed melon and fruit vegetable skins and the crushed Chinese herbal medicine dregs into 110 parts of biogas residue dry base, adding EM (effective microorganisms) bacterial liquid, uniformly mixing and stirring to obtain mixed feed, adjusting the adding amount of the EM bacterial liquid to ensure that the water content of the mixed feed is 76%, adding the obtained mixed feed into a black soldier fly breeding pond to breed black soldier fly larvae, putting 4-day-old black soldier fly larvae into the breeding pond, adding 0.03kg of 4-day-old black soldier fly larvae into each kg of the mixed feed, breeding at the temperature of 30-35 ℃, feeding the black soldier fly larvae into a prepuputation stage to obtain a pest residue mixture, and separating the pest residues to obtain the black soldier fly larvae and the feed residues.
Example 3
Mushroom dregs and Chinese herbal medicine dregs are used as feeding auxiliary materials
Respectively weighing 50 parts of mushroom residues and 50 parts of Chinese herbal medicine residues, respectively crushing, crushing to a granularity of 60 meshes, adding the crushed mushroom residues and the crushed Chinese herbal medicine residues into 120 parts of biogas residue dry base, adding EM (effective microorganisms) liquid, mixing and stirring uniformly to obtain mixed feed, adjusting the addition amount of the EM liquid to ensure that the water content of the mixed feed is 77%, adding the obtained mixed feed into a culture pond of hermetia illucens to culture the hermetia illucens larvae, putting 4-day-old hermetia illucens larvae into the culture pond, adding 0.04kg of 4-day-old hermetia larvae into each kg of the mixed feed, feeding the mixture at the temperature of 30-35 ℃ under the condition of relative humidity of 70-85%, obtaining a residue mixture when the larvae enter a prepupation stage, and separating the residue to obtain the hermetia illucens larvae and feed residues.
Example 4
Animal internal organs are used as feeding auxiliary materials
Weighing 100 parts of animal viscera, crushing the animal viscera into 100 meshes, adding the crushed animal viscera into 130 parts of biogas residue dry base, adding EM (effective microorganisms) liquid, mixing and stirring the mixture uniformly to obtain mixed feed, adjusting the addition of the EM liquid to ensure that the water content of the mixed feed is 78%, adding the obtained mixed feed into a Hermetia illucens breeding pond to breed the Hermetia illucens larvae, putting 4-day-old Hermetia illucens larvae into the breeding pond, adding 0.04kg of 4-day-old Hermetia illucens larvae into each kg of the mixed feed, breeding the Hermetia illucens larvae at 30-35 ℃ under the condition of relative humidity of 70-85%, obtaining a residue mixture when the larvae enter a prepupation stage, and separating the residue to obtain the Hermetia illucens larvae and the feed residue.
Example 5
Adopting melon, fruit and vegetable skin, mushroom residue and Chinese herbal medicine residue as feeding auxiliary materials
Respectively weighing 20 parts of melon and fruit vegetable skins, 30 parts of mushroom residues and 50 parts of Chinese herbal medicine residues, respectively crushing, wherein the granularity of the crushed materials is 100 meshes, adding the crushed mushroom residues and the crushed Chinese herbal medicine residues into 150 parts of biogas residue dry base, adding EM (effective microorganisms) bacterial liquid, uniformly mixing and stirring to obtain mixed feed, adjusting the adding amount of the EM bacterial liquid to enable the water content of the mixed feed to be 80%, adding the obtained mixed feed into a hermetia illucens breeding pond to breed the hermetia illucens larvae, putting 4-day-old hermetia illucens larvae into the breeding pond, adding 0.05kg of 4-day-old hermetia larvae into each kg of the mixed feed, breeding at the temperature of 30-35 ℃, feeding the larvae under the condition of 70-85% relative humidity, obtaining an insect residue mixture after the larvae enter a prepuputation stage, and separating the insect residues to obtain the hermetia illucens larvae and feed residues.
The untreated biogas residues are used as comparative examples, the untreated biogas residues and the feed residues finally produced in examples 1 to 5 are respectively subjected to freezing, drying and sieving treatment, and the heavy metal content and the antibiotic content are respectively detected after the treatment, and the results are as follows:
based on data, the treatment method can effectively remove harmful components in the biogas residues, and meanwhile, the biogas residues can also be used for feeding hermetia illucens larvae, and the treatment method is mild, environment-friendly, high in utilization efficiency and good in application prospect.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (9)
1. A method for treating biogas residues by using hermetia illucens is characterized by comprising the following steps:
adding crushed feeding auxiliary materials and EM bacterial liquid into biogas residues to prepare mixed feed;
step two, adding the mixed feed obtained in the step one into a black soldier fly breeding pond, breeding black soldier fly larvae until the larvae enter a prepupa stage, and obtaining a residue mixture;
and step three, separating the worm residue mixture obtained in the step two to obtain hermetia illucens larvae and feed residues.
2. The method for treating biogas residue with black soldier flies in claim 1, wherein in the first step, the feeding auxiliary materials are one or a mixture of several of animal viscera, herb residues, mushroom residues, fruits and vegetable skins.
3. The method for treating biogas residue with hermetia illucens according to claim 1, wherein in the first step, the granularity of the crushed feeding auxiliary materials is 60-100 meshes.
4. The method for treating biogas residue with hermetia illucens according to claim 1, wherein the biogas residue is obtained by anaerobic fermentation of one or more of kitchen waste, wet waste, poultry manure and straw.
5. The method of claim 1, wherein the mixed feed obtained in the first step has a water content of 75-80%.
6. The method for treating biogas residues by using hermetia illucens as claimed in claim 1, wherein in the first step, the dry basis mass ratio of the feeding auxiliary materials to the biogas residues is 1: (1-1.5).
7. The method of claim 1, wherein the hermetia illucens larvae bred in step two are 4-day-old larvae.
8. The method for processing the biogas residue with the hermetia illucens as claimed in claim 1, wherein in the second step, the temperature for breeding the hermetia illucens larvae is 30-35 ℃, and the relative humidity of air is 70-85%.
9. The method for treating biogas residue with hermetia illucens according to claim 1, wherein in the second step, the feed is mixed: the mass ratio of the hermetia illucens larvae is 1: (0.02-0.05).
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010855927.9A CN111990340A (en) | 2020-08-24 | 2020-08-24 | Method for treating biogas residues by using hermetia illucens |
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| CN202010855927.9A CN111990340A (en) | 2020-08-24 | 2020-08-24 | Method for treating biogas residues by using hermetia illucens |
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| CN202010855927.9A Pending CN111990340A (en) | 2020-08-24 | 2020-08-24 | Method for treating biogas residues by using hermetia illucens |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114586744A (en) * | 2020-12-04 | 2022-06-07 | 中国科学院分子植物科学卓越创新中心 | Method for simultaneously realizing resource treatment of kitchen waste and traditional Chinese medicine dregs through hermetia illucens |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105945031A (en) * | 2016-05-19 | 2016-09-21 | 湖南民兴智能科技有限公司 | Kitchen garbage treatment method |
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| CN110115252A (en) * | 2019-06-27 | 2019-08-13 | 天津易世东生态科技有限公司 | A kind of method of black soldier flies biodegrade kitchen garbage |
| CN110250115A (en) * | 2019-07-23 | 2019-09-20 | 南宁利腾农业科技有限公司 | In the method for black soldier flies processing pigeon dung |
| CN110484570A (en) * | 2019-09-03 | 2019-11-22 | 江南大学 | A method of utilizing kitchen garbage co-producing hydrogen and insect protein |
| AU2020101383A4 (en) * | 2020-07-16 | 2020-08-20 | Zhejiang University Of Science And Technology | Biogas residue-based mechanized earthworm composting breeding system |
-
2020
- 2020-08-24 CN CN202010855927.9A patent/CN111990340A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105945031A (en) * | 2016-05-19 | 2016-09-21 | 湖南民兴智能科技有限公司 | Kitchen garbage treatment method |
| CN109090043A (en) * | 2018-07-30 | 2018-12-28 | 开平市华声生物科技有限公司 | A kind of black soldier flies larva cultural method |
| CN110115252A (en) * | 2019-06-27 | 2019-08-13 | 天津易世东生态科技有限公司 | A kind of method of black soldier flies biodegrade kitchen garbage |
| CN110250115A (en) * | 2019-07-23 | 2019-09-20 | 南宁利腾农业科技有限公司 | In the method for black soldier flies processing pigeon dung |
| CN110484570A (en) * | 2019-09-03 | 2019-11-22 | 江南大学 | A method of utilizing kitchen garbage co-producing hydrogen and insect protein |
| AU2020101383A4 (en) * | 2020-07-16 | 2020-08-20 | Zhejiang University Of Science And Technology | Biogas residue-based mechanized earthworm composting breeding system |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114586744A (en) * | 2020-12-04 | 2022-06-07 | 中国科学院分子植物科学卓越创新中心 | Method for simultaneously realizing resource treatment of kitchen waste and traditional Chinese medicine dregs through hermetia illucens |
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Effective date of registration: 20220324 Address after: 430000 1st floor, test center building of Luther environmental protection industry R & D base, No. 51, Jiulonghu street, East Lake New Technology Development Zone, Wuhan, Hubei Province Applicant after: Luther biological environmental protection technology (Wuhan) Co.,Ltd. Address before: 430000 south of Gaoxin 2nd Road and east of Guanggu 7th Road, Donghu New Technology Development Zone, Wuhan City, Hubei Province Applicant before: PROO Biotechnology (Wuhan) Co.,Ltd. |
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Application publication date: 20201127 |