CN115067285B - Method for obtaining white star flower scarab breeding furgus lucorum by using cane shoot straw - Google Patents
Method for obtaining white star flower scarab breeding furgus lucorum by using cane shoot straw Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention relates to the technical field of breeding of lygus lucorum, and discloses a method for obtaining white star flower scarab breeding of lygus lucorum by using cane shoot straw, wherein S1 waste is pretreated; s2, performing transformation on insects in the environment through abdomen; s3, artificially breeding natural enemy insects; s4, refrigerating and preserving the prey, treating cane shoot straw by adopting white star scara beetle larvae through belly, producing transformed larvae and pupae for breeding the horn Li Chun, improving the survival rate and fertility of the horn, reducing the culture cost and changing waste into valuables; the insect manure can be directly applied to farmland bio-organic fertilizer, and the additional yield of breeding is increased. The method is simple to operate, economical and practical, is environment-friendly, can realize innocuous treatment and resource utilization of cane shoot straw waste, can reduce the damage of the adult white star tortoises, can be used for various purposes such as population expansion or biological characteristic research or field release prevention and control of pests, and has remarkable ecological, social and economic benefits.
Description
Technical Field
The invention relates to the technical field of breeding of lygus lucorum, in particular to a method for obtaining white star flower scarab breeding of lygus lucorum by using cane shoot straw.
Background
The fork angle Li Chun Eocantheconidea furcellata (Wolff) belongs to Hemiptera of the order Hemiptera, pentatomidae, asprinae of the sub-family Yi Zhu, li Chun belong to Eocuanthecona, and is widely distributed in the south China, has a wide predatory range, relates to more than 40 larvae of lepidoptera, coleoptera, hymenoptera, hemiptera and the like, and is an important natural enemy insect for agricultural, forest, fruit and tea crop pests. Especially has strong predatory ability for lepidoptera pests such as asparagus caterpillar, plutella xylostella, prodenia litura, spodoptera frugiperda, tea geometrid and the like, and has good popularization prospect and application potential. At present, the scale breeding artificial feed of the lygus lucorum has higher cost and lower yield and survival rate, so that the yellow meal worm is mostly used as a prey for feeding.
Cane shoots are perennial shallow water gramineous plants and are planted in large areas in Anhui, zhejiang and other places in China. It is rich in saccharide, organic nitrogen, fat, protein, cellulose, amino acid, etc. and may be used as food, dish, feed, etc. and has high economic benefit. According to investigation, the yield of cane shoots accounts for 50-70% of the total mass of plants, and about 5000-kg straws can be produced in each mu of cane shoot field. Because cane shoot straw biomass is large, serious environmental pollution and resource waste can be caused by random discarding or incineration, and the existing treatment mode such as direct returning to the field can increase the disease and pest risks of the next crop; the high-temperature composting process is complex in flow and low in comprehensive utilization efficiency. Therefore, the exploration of innocent treatment and resource utilization methods of cane shoot straws is a problem to be solved urgently.
White star flower tortoise Protaetia brevitarsis (Lewis) belongs to Coleoptera, ceroniidae, eupolyphaga. The larvae have the characteristics of corrosion, large eating amount, high conversion speed and the like, can well convert agricultural organic wastes such as corn stalks, edible fungus chaff, apple wastes and the like, and the worm manure converted by the larvae through abdomen has good aggregate structure characteristics, and can be directly applied as farmland organic fertilizer. The white star flower scarab larvae and pupae have higher protein content, contain multiple vitamins, minerals and microelements, can meet the nutrition requirements of the lygus lucorum in different development stages, and can replace yellow meal worm to be used as a good host for artificial breeding of the lygus lucorum.
The method adopts the white star flower scarab to eat cane shoot straw waste, the converted larva or pupa is used as breeding feed of the lygus lucorum, and the insect manure is used as the bio-organic fertilizer. The method not only provides a new idea for recycling the cane shoot straw waste, but also can lighten the harm of the adult white star tortoises, improve the survival rate and fertility of the useful natural enemy, namely the lygus lucorum, and the bred adults can be used for various purposes such as population expansion or biological characteristic research or field release prevention and control of pests, and have remarkable ecological, social and economic benefits.
Disclosure of Invention
(one) solving the technical problems
Aiming at the defects of the prior art, the invention provides a method for obtaining the breeding of the lygus lucorum by using the cane shoot straw.
In order to achieve the above purpose, the present invention provides the following technical solutions: a method for obtaining white star flower scarab breeding furgus lucorum by using cane shoot straws,
s1, pretreatment of waste: crushing the harvested cane shoot straws uniformly by adopting a small crusher;
s2 transformation of insects in environment through abdomen: inoculating white-star flower scarab larvae in a conversion treatment container, feeding white-star flower scarab larvae by using crushed straws in S1 according to the mass ratio of 1:8-10, and treating for 30-50 days, wherein insect manure is used as a bio-organic fertilizer, and the larvae and pupae are used for artificial breeding;
s3, artificial breeding of natural enemy insects: in the breeding device, the white star flower scarab pupa or the white star flower scarab larva harvested in the S2 are placed for feeding the lygus lucorum in a certain proportion every day, and meanwhile, a water source is supplemented. 1-2 years nymphs: 1 worm pupa is put in every 5-10 heads; 3-5 year old nymphs: 1 larva is put in every 3-5 heads; adults: 1-2 larvae are put in every 1 head until spawning; collecting eggs by adopting folding paper sheets;
s4, refrigerating and preserving the hunting: and (3) placing the redundant larva and pupa of the scarab beetle in the S3 in a low-temperature environment for cold storage.
Preferably, the S1 cane shoot straw is crushed to be 0.5-2 cm in length.
Preferably, the S2 environment condition is 25-30 ℃, the humidity is 70% +/-10%, and the sun is dark.
Preferably, the S2 conversion treatment container is a plastic box, the length of the plastic box is 150-200 cm, the width of the plastic box is 80-100 cm, the height of the plastic box is 60-80 cm, the plastic box is covered with 20-30 cm, water is added to adjust the humidity to 10-20%, and crushed straws are uniformly paved on the soil surface.
Preferably, the larva of the S2 white star flower scarab beetle is 2 years old or 3 years old, according to 2-4 kg/m 2 And (5) inoculating.
Preferably, the S2 is used for separating and collecting the insect manure through a 30-45-mesh screen.
Preferably, the temperature of the artificial climate box is adjusted to 25-30 ℃, the humidity is 70% +/-10%, the photoperiod is 14 L:10D, L is short-term Light, D is short-term Dark, and the illumination intensity is 30000-40000 Lux.
Preferably, the S3 breeding device is an 80-mesh nylon net cage with a zipper and an operation port (the length is 50-60 cm, the width is 30-40 cm and the height is 20-30 cm), adults and nymphs are fed separately, and nymphs with consistent ages are fed together.
Preferably, the S3 is selected to feed the pupae or the initial 3-year larva which just enter the eclosion state; and S3, placing the absorbent cotton balls after water absorption into a nylon net cage to supplement water sources.
Preferably, the refrigerating and preserving temperature of the S4 is 4-8 ℃, the preserving humidity is 50% +/-10%, and the preserving time is not more than 30 days.
Compared with the prior art, the invention provides a method for obtaining the breeding of the lygus lucorum by using the cane shoot straw, which has the following beneficial effects:
the wild rice stem straw is processed by the white star flower scarab larvae through the abdomen, and the transformed larvae and pupae are produced for raising and breeding the horn Li Chun, so that the survival rate and the fertility of the wild rice stem straw are improved, the cultivation cost is reduced, and waste is changed into valuable; the insect manure can be directly applied to farmland bio-organic fertilizer, and the additional yield of breeding is increased. The method is simple to operate, economical and practical, is environment-friendly, can realize innocuous treatment and resource utilization of cane shoot straw waste, can reduce the damage of the adult white star tortoises, can be used for various purposes such as population expansion or biological characteristic research or field release prevention and control of pests, and has remarkable ecological, social and economic benefits.
Drawings
FIG. 1 is a flow chart of a method for obtaining breeding stinkbug of white star tortoises by using cane shoot straw;
FIG. 2 is a table diagram showing the effect of feeding white flower beetle pupae on the growth and development of the cross horn Li Chun nymphs in the invention;
FIG. 3 is a chart showing the effect of feeding white-star scarab beetle pupae on the physiological parameters of the adult cross Li Chun and the hatching rate of offspring eggs.
Description of the embodiments
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to figures 1-2 of the drawings,
examples
Crushing and pre-treating the harvested cane shoot straws to ensure that the length of the cane shoot straws reaches 0.5-1 cm, covering 20-30 cm with soil in a plastic box (150-200 cm long, 80-100 cm wide and 60-80 cm high), adding water to adjust the humidity to 10-20%, and regulating the humidity according to 2-4 kg/m 2 Inoculating 3-instar larvae of scarab beetles, uniformly spreading crushed straws on the soil surface (the ratio of insect materials is 1:8), controlling the environmental conditions to be 25-30 ℃ and the humidity to be 70% +/-10%, darkening and shading, separating the larvae and the insect manure by adopting a screen with 30-45 meshes after conversion treatment for 30 days, and using the obtained insect manure as a bio-organic fertilizer, wherein the larvae and pupae are used for artificial breeding.
The 80 mesh nylon net cage with zipper and operation mouth is used for cultivating (length 50-60 cm, width 30-40 cm, height 20-30 cm) fork angle Li Chun, and absorbent cotton balls are added to supplement water source. Regulating the temperature of a manual climate box to 25-30 ℃, controlling the humidity to 70% +/-10%, and illuminating 14L to 10D, wherein the illumination intensity is 30000-40000 Lux. Selecting white flower scarab beetle pupa or 3-year-old initial larva which just enters into the eclosion state, feeding according to a certain proportion, and 1-2-year-old larva: 1 pupa is put in every 5 nymphs; age 3-5: 1 larva is put in every 3 nymphs; adults: every 1 head is put with 2 larvae until spawning. Fresh pupae and larvae are replenished every day, absorbent cotton balls are replaced, adult and nymphs of the horn Li Chun are fed separately, nymphs with consistent age are fed together, and eggs are collected by adopting folded paper sheets. And (5) placing the redundant larva and pupa of the scarab beetle at the temperature of 4-8 ℃ and the humidity of 50% +/-10% for preservation, wherein the time is not more than 30 days.
Examples
Crushing and pre-treating the harvested cane shoot straws to ensure that the length of the cane shoot straws reaches 1-2 cm, earthing 20-30 cm in a plastic box (150-200 cm long, 80-100 cm wide and 60-80 cm high), adding water to adjust the humidity to 10-20%, and regulating the humidity to 2-4 kg/m 2 Inoculating 2-instar larvae of scarab beetles, uniformly spreading crushed straws on the soil surface (the ratio of insect materials is 1:10), controlling the environmental conditions to be 25-30 ℃ and the humidity to be 70% +/-10%, and separating the larvae and the insect manure by adopting a 30-45 mesh screen after darkness and light shading conversion treatment for 50 days, thereby obtaining the scarab beetlesThe insect manure is used as a biological organic fertilizer, and the larvae and pupae are used for artificial breeding.
The 80 mesh nylon net cage with zipper and operation mouth is used for cultivating (length 50-60 cm, width 30-40 cm, height 20-30 cm) fork angle Li Chun, and absorbent cotton balls are added to supplement water source. Regulating the temperature of a manual climate box to 25-30 ℃, controlling the humidity to 70% +/-10%, and illuminating 14L to 10D, wherein the illumination intensity is 30000-40000 Lux. Selecting white flower scarab beetle pupa or 3-year-old initial larva which just enters into the eclosion state, feeding according to a certain proportion, and 1-2-year-old larva: 1 pupa is put in every 10 nymphs; age 3-5: 1 larva is put in every 5 heads; adults: 1 larva is put in every 1 head until spawning. Fresh pupae and larvae are replenished every day, absorbent cotton balls are replaced, adult and nymphs of the horn Li Chun are fed separately, nymphs with consistent age are fed together, and eggs are collected by adopting folded paper sheets. And (5) placing the redundant larva and pupa of the scarab beetle at the temperature of 4-8 ℃ and the humidity of 50% +/-10% for preservation, wherein the time is not more than 30 days.
Control example 1 (Artificial feed)
Reference is made to patent application 201810311825.3 for a slightly modified artificial feed formulation for lygus lucorum. The artificial feed formula comprises 30 parts by weight of maggot powder, 10 parts by weight of egg yolk, 8 parts by weight of honey, 5 parts by weight of royal jelly, 3 parts by weight of yeast powder, 10 parts by volume of fresh milk and 5 parts by volume of vegetable juice (the ratio of 1 part by weight to 1 part by volume=1 g/mL). And (3) placing the mixture in a magnetic stirrer, stirring at 25-30 ℃ for 20-30 min to uniformly mix, and naturally cooling to obtain the artificial feed. The 80 mesh nylon net cage with zipper and operation mouth is used for cultivating (length 50-60 cm, width 30-40 cm, height 20-30 cm) fork angle Li Chun, and absorbent cotton balls are added to supplement water source. Regulating the temperature of a manual climate box to 25-30 ℃, controlling the humidity to 70% +/-10%, and adding artificial feed for feeding when the illumination is 14L to 10D and the illumination intensity is 30000-40000 Lux. Fresh feed is replenished every day, absorbent cotton balls are replaced, adult nymphs and nymphs of the horn Li Chun are fed separately, nymphs with consistent age are fed together, and eggs are collected by using folded paper sheets.
Control example 2 (yellow mealworm)
The 80 mesh nylon net cage with zipper and operation mouth is used for cultivating (length 50-60 cm, width 30-40 cm, height 20-30 cm) fork angle Li Chun, and absorbent cotton balls are added to supplement water source. Regulating the temperature of a manual climate box to 25-30 ℃, controlling the humidity to 70% +/-10%, and illuminating 14L to 10D, wherein the illumination intensity is 30000-40000 Lux. Fresh yellow meal worm living pupae or 2-4-year-old larvae are selected to be fed according to a certain proportion, and the ages of 1-2 are as follows: 1 pupa is put in every 5 nymphs; age 3-5: 1 larva is put in every 3 nymphs; adults: every 1 head is put with 2 larvae until spawning. Fresh pupae and larvae are replenished every day, absorbent cotton balls are replaced, adult and nymphs of the horn Li Chun are fed separately, nymphs with consistent age are fed together, and eggs are collected by adopting folded paper sheets.
4 groups of lygus lucorum, 200 heads per group, 3 replicates per group were fed with the feed formulations and methods of example 1, example 2, control 1, control 2, respectively. After the test is finished, 50 heads are randomly picked up for each repetition, and the physiological parameter differences of nymphs and adults after different foods are picked up by statistical analysis. The data are shown in figures 2-3 below.
The data in figures 2-3 are mean ± standard error, and the different lower case letters after the same column of data represent significant differences after ANOVA single factor analysis of variance and Turkey's multiple comparisons, p <0.05.
From the results of the table, compared with the artificial feed, the survival rate of the nymphs, the weight of the adults, the life span of the females, the spawning amount and the egg hatching rate of the example 1 are obviously improved, and the period of the total development duration of the nymphs and the spawning early period are obviously shortened. The difference between the example 1 and the yellow meal worm control group is not great, so that the yellow meal worm pupa feeding fork angle Li Chun can be completely replaced, and the normal growth and development of nymphs and adults are ensured. Compared with the example 2, the life span and spawning amount of the female insects in the example 1 are obviously improved, the development period of nymphs and the spawning period of adults are obviously shortened, so that better effect can be obtained in practical application by putting and feeding according to the example 1.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (5)
1. A method for obtaining a white star flower scarab breeding furgus lucorum by using cane shoot straw is characterized by comprising the following steps:
s1, pretreatment of waste: crushing the harvested cane shoot straws uniformly by adopting a small crusher;
s2 transformation of insects in environment through abdomen: inoculating white-star flower scarab larvae in a conversion treatment container, feeding white-star flower scarab larvae by using crushed straws in S1 according to the mass ratio of 1:8-10, and treating for 30-50 days, wherein insect manure is used as a bio-organic fertilizer, and the larvae and pupae are used for artificial breeding;
s3, artificial breeding of natural enemy insects: in the breeding device, the white star flower scarab pupa or larva harvested in the S2 is placed for feeding the lygus lucorum in a certain proportion every day, and meanwhile, a water source is supplemented, and 1-2 years old nymphs: 1 worm pupa is put in every 5-10 heads; 3-5 year old nymphs: 1 larva is put in every 3-5 heads; adults: 1-2 larvae are put in every 1 head until spawning; collecting eggs by adopting folding paper sheets;
s4, refrigerating and preserving the hunting: placing the redundant larva and pupa of the scarab beetle in the S3 in a low-temperature environment for cold storage;
crushing the S1 cane shoot straws to a length of 0.5-2 cm;
the environmental condition of the S2 is 25-30 ℃, the humidity is 70% +/-10%, and the environment is dark and light-proof;
the S2 conversion treatment container is a plastic box, the length of the container is 150-200 cm, the width of the container is 80-100 cm, the height of the container is 60-80 cm, the soil is covered with 20-30 cm, water is added to adjust the humidity to 10-20%, and crushed straws are uniformly paved on the soil surface;
the larva of the S2 white star flower scarab beetle is 2 years old or 3 years old, according to 2-4 kg/m 2 Inoculating;
and S2, separating and collecting the insect manure through a 30-45-mesh screen.
2. The method for obtaining the white star tortoise breeding furuncle stink by utilizing cane shoot straws according to claim 1, which is characterized by comprising the following steps: and S3, regulating the temperature of the artificial climate box to be 25-30 ℃ and the humidity to be 70% +/-10%, wherein the photoperiod is 14 L:10D, L is short-term Light, D is short-term Dark, and the illumination intensity is 30000-40000 Lux.
3. The method for obtaining the white star tortoise breeding furuncle stink by utilizing cane shoot straws according to claim 1, which is characterized by comprising the following steps: the S3 breeding device is an 80-mesh nylon net cage with a zipper and an operation port, the length is 50-60 cm, the width is 30-40 cm, the height is 20-30 cm, adults and nymphs are separately bred, and the nymphs with the same age are bred together.
4. The method for obtaining the white star tortoise breeding furuncle stink by utilizing cane shoot straws according to claim 1, which is characterized by comprising the following steps: s3, selecting the insect pupae or the initial larvae of 3 years to feed; and S3, placing the absorbent cotton balls after water absorption into a nylon net cage to supplement water sources.
5. The method for obtaining the white star tortoise breeding furuncle stink by utilizing cane shoot straws according to claim 1, which is characterized by comprising the following steps: the refrigerating and preserving temperature of the S4 is 4-8 ℃, the preserving humidity is 50% +/-10%, and the preserving time is not more than 30 days.
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