CN100388884C - Method for breeding pyemote, and its culturing vessel, and method for releasing same - Google Patents

Method for breeding pyemote, and its culturing vessel, and method for releasing same Download PDF

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CN100388884C
CN100388884C CNB2005100128538A CN200510012853A CN100388884C CN 100388884 C CN100388884 C CN 100388884C CN B2005100128538 A CNB2005100128538 A CN B2005100128538A CN 200510012853 A CN200510012853 A CN 200510012853A CN 100388884 C CN100388884 C CN 100388884C
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mite
culture dish
plastic culture
grub
larva
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CN1757283A (en
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段东红
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Abstract

The present invention relates to a method for breeding pyemotes, a pyemotes culturing vessel, and a method for releasing pyemotes. The method for breeding the pyemotes is that development pyemotes are preserved in cold storage boxes with-5 to-15 DGE C around April to May. Bee aging larva, or pupae of silkworm, or grub are used as replacing hosts which are put into internal plastic petri dishes. Seed pyemotes is preferably selected from the excellence selecting methods of creeping, or hunger endurance, or cold endurance. Then collected hosts are put into the internal plastic petri dishes and are used for culturing the second generation of the pyemotes through times quantity inoculation and test tube culture. The pyemotes culturing vessel is composed of a stainless steel culturing dish and a plurality of pairs of internal and external plastic petri dishes which are stuck together; the positions close to the bottom parts of the external plastic petri dishes are provided with via holes. The pyemotes is released in the method that the pyemotes inside test tubes are poured on a pyemotes releasing box of which one side is opened. The pyemotes breeding method has the advantage of low cost and is suitable for industrial production breeding. The pyemotes culturing vessel is suitable for the pyemotes producing requirements of industrial production, and the method for releasing the pyemotes is favorable for the pyemotes quickly creeping to look for the hosts.

Description

A kind of Pu mite mating system and Pu mite incubator thereof
Technical field
The present invention relates to a kind of Pu mite mating system and Pu mite incubator thereof.
Technical background
Trunk borer is a kind of serious harm forest plants, the forestry pest that threatens forest ecology safety.Trunk borer breaks out often, and regular meeting causes the death of forest large tracts of land.Trunk borer is sought hidden life, and in its whole life from generation to generation, the overwhelming majority time all endangers in host's trunk or root, adopts the common method of preventing and treating of spraying insecticide that it is cut any ice hardly.Therefore, the control of trunk borer not only difficulty is very big, and the control cost is very high, and control efficiency is undesirable, and uses chemical pesticide also can cause serious environmental to pollute in a large number.Utilizing natural enemy insect control trunk borer is the developing direction of biological control, also is the key that continues, stablizes, effectively controls the forestry pest.The so just natural enemy insect of Pu mite (Pymeotesscolyti), it all has the good control efficiency of tool to multiple trunk borer.So, cultivate, breeding Pu mite be prevent and treat trunk borer at present, a key areas of the ecotope that conserves forests.At present; the method of domestic existing propagation in scale Pu mite; this method adopts following several alternative hosts to breed the Pu mite; weak point is all arranged: adopt the Cathay poplar roundheaded borer to breed the Pu mite; need a large amount of parasitic naturally Cathay poplar roundheaded borers of natural world of gathering; this not only is subjected to the influence of natural conditions very big, and the quantity of the Cathay poplar roundheaded borer that natural world provided be with originally can't meeting the demands, and the worm source can't be guaranteed.Adopt bloom first or cute conjunctivitis bee pupa to breed the Pu mite, its Pu mite that breeds out amount is too little, does not have practical significance on producing.Adopt the semanotus bifasciatus larva to breed the Pu mite, the requirement of propagation in scale Pu mite seldom can't be satisfied in natural semanotus bifasciatus larva source, can only lean on artificial breeding to solve.It is earlier cypress to be made trap-tree that the semanotus bifasciatus larva breeds, and inoculates semanotus bifasciatus with trap-tree then, breeds the Pu mite with the semanotus bifasciatus larva again.Adopt this method to breed the Pu mite, not only need to cut down the gloomy plant cypress of a large amount of evergreen preciousnesses and make trap-tree, and the semanotus bifasciatus amount that is produced is little, production scale also is difficult to do greatly, and cost is also higher.On average inoculate 10 semanotus bifasciatus adults by every cypress, the male and female ratio is 5: 5, and every female worm on average lays eggs 71, and the acquisition rate by 70% is calculated, every larva that cypress can be gathered 250 semanotus bifasciatuss approximately.The larva of each semanotus bifasciatus can breed out 100,000 Pu mites, prevents and treats strain trees and needs 200,000 Pu mites, promptly needs 2 semanotus bifasciatus larvas.The semanotus bifasciatus larva that every cypress produced can be satisfied the control of 125 strain trees.About 4,000,000 hectares of China annual generation moth dryness forestry pest, close 6,000 ten thousand mu, every mu of average 100 strains tree, the worm strain rate that has by 10% is calculated, there are 600,000,000 strain forests to need control, need felling 4,800,000 strain cypresses altogether, close 1 mu of forest by per 100 strain cypresses, need 4.8 ten thousand mu of cypresses of felling altogether, this is impossible fully.Cypress is the precious evergreen species of China, belongs to rare or endangered species, and is very rare in China's quantity, and basic not enough felling is used for breeding the Pu mite.Simultaneously, it is also very big to breed the workload of semanotus bifasciatus larva.
Escape phenomenon can take place because of seeking the host in the Pu mite in the process of breeding, therefore will use special-purpose incubator.Method commonly used at present is to set up little cultivation platform in common culture dish, smears repellant on the outer wall of culture dish, prevents that the Pu mite from escaping.Smear that repellant is more time-consuming takes a lot of work, be not suitable for production application on the industry.
Summary of the invention
In order to overcome the above-mentioned deficiency of existing Pu mite mating system, the invention provides a kind of need not to cut down a large amount of precious forests, the Pu mite mating system that is suitable for using on the industry that cost is low.
The present invention provides a kind of Pu mite incubator that is suitable for the industry instructions for use simultaneously.
Pu mite mating system of the present invention is being prepared the alternative host of Pu mite, is selecting excellent kind of mite, is being bred the Pu mite, in the preservation of Pu mite, the transportation of Pu mite and the Pu mite dispose procedure, can directly operate with alternative host.
The key of breeding the Pu mite is to seek suitable alternative host, and the alternative host who can be used as the Pu mite has thousands of kinds, and the applicant is through years of researches and test, and the mature larva of selection honeybee, the pupa of silkworm and grub are as the alternative host of Pu mite.
Introduce the technological process of this Pu mite mating system by Fig. 1.At first be to prepare Pu mite 1 and gather host 2 among the figure, gather the host five kinds of modes are arranged, can directly buy the mature larva 3 of honeybee or gather the pupa 4 of silkworm, also can in ground, gather grub 5, also orientable numerous honeybee 6 and breed grub 7, then alternative host and Pu mite are put into feed chamber constant temperature refrigeration 8, after preferably Pu mite 9 inoculates 10 with a times amount, put into culturing room's constant temperature culture 11, after culturing room's test tube cultivation 12 goes out the Pu mite then, put into temporary room constant temperature refrigeration 13, put into finished room constant temperature refrigeration 14.After the constant temperature transportation 15, discharge natural enemy 16 to the forest land during use.
This Pu mite mating system comprises following steps successively:
One, prepares the Pu mite
The 4--5 month, buy the Pu mite developed into full swollen abdomen body in the forestry PCO phase, be put in-preserve in the refrigerating box of 5-15 ℃, select to plant a mite and prepare for carrying out orientation.
Two, prepare the alternative host of Pu mite
The present invention selects the pupa of the mature larva of honeybee or silkworm or the grub alternative host as breeding Pu mite for use.The mature larva of honeybee can directly be bought from the bee-keeping family, and grub can be gathered in farmland or forest land, and the pupa of silkworm is directly purchased to the silkworm raiser.
1, prepares Pu mite incubator
Pu mite incubator is by a stainless steel culture plate and some plastic culture dish is formed, every pair of plastic culture dish is made up of inside and outside two plastic culture dish, the interior plastic culture dish of 9 centimetres of diameters is bonded at glue in the middle of the outer plastic culture dish of 12 centimetres of diameters, the height of outer plastic culture dish is a little more than the interior plastic culture dish of the inside, and the plastic culture dish sidewall has the through hole of 2 millimeters on a circle by the position of 1 millimeter of bottom outside.During use, interior plastic culture dish that bonds together and outer plastic culture dish are put into the stainless steel culture plate, 62 centimetres of the length of stainless steel culture plate, wide be 38 centimetres, high 5 centimetres, inside and outside each stainless steel culture plate is put 15 to plastic culture dish, in the stainless steel culture plate, add clear water, the height of clear water is half of outer plastic culture dish, can prevent that the Pu mite from escaping.The glass culture dish that the also available appearance and size of outer plastic culture dish that the interior plastic culture dish that diameter is 9 centimetres, diameter are 12 centimetres is identical replaces, and the stainless steel culture plate can replace with appearance and size identical plastic culture dish dish or enamel culture plate.
2, prepare to substitute the host with any method in following three kinds of methods
The preparation of the mature larva of a, honeybee
Open beehive, the honeybee spleen of selecting to have developed into mature larva mostly takes out together with the honeybee frame, takes out mature larva gently with tweezers and put into plastic culture dish from honeycomb.The honeycomb that has sealed La Gai can remove La Gai with tweezers earlier and take out mature larva more gently.Plastic culture dish is put into 30-40 mature larva in each, puts into temperature 10-15 ℃, and relative moisture is preserved stand-by in the feed chamber of 55%-70%.
The preparation of b, mulberry silkworm chrysalis
Silkworm have silkworm in spring, summer silkworm and the autumn silkworm, its pupa all can use, and can choose according to actual conditions.Select just to have told silk, the mulberry cocoon that the cocoon shell has just been pupated is firmly put into temperature 10-15 ℃, and relative moisture is preserved stand-by in the feed chamber of 55%-70%.During inoculation, cut off the mulberry cocoon, take out mulberry silkworm chrysalis and put into plastic culture dish with scissors.Putting into the 10-20 mulberry silkworm chrysalis in the plastic culture dish in each waits to inoculate.Put into temperature 10-15 ℃ again, relative moisture is preserved stand-by in 55%70% feed chamber.
The preparation of C, grub
The grub of gathering in soil layer 15 cm range is collected, remove soil and weeds, put into plastic culture dish, put into the 30-40 grub in the plastic culture dish in each.Put into temperature 10-15 ℃ again, relative moisture is preserved stand-by in the feed chamber of 55%-70%.
Three, preferably plant mite
The present invention's power of creeping selects excellent, anti-famine to select excellent, the cold-resistant excellent three kinds preferred three kinds methods of selecting, and can adopt following arbitrary method preferably to plant mite according to different purposes, also can adopt following three kinds of methods combination preferably to plant mite.
1, the power of creeping is selected excellent
The Pu mite of buying is placed on 50 millimeters-80 millimeters of diameters, long 1000 millimeters-1500 millimeters a end of lucite tube, and pupa or grub that the other end is put into larva of bee or silkworm substitute the host, and Pu mite and above-mentioned alternative host's ratio is 10000--20000: 1.The two ends of lucite tube are clogged with rubber stopper, prevent that the Pu mite from runing away.Again lucite tube is placed on 20 ℃-30 ℃ of temperature, the culturing room of relative moisture 55%-80%, cleaning, ventilation cultivates.After 10-18 days, the Pu mite of lucite tube one end colonizes in the pupa of the mature larva of honeybee or silkworm or grub substitutes on the pin main body and after the surface that substitutes the host is fixing, above-mentioned alternative host is put into test tube, each test tube is put into larva or 2-5 mulberry silkworm chrysalis or 2-10 grub of 2-10 honeybee, again test tube is placed on temperature 20-30 ℃, the cleaning of relative moisture 55%-80%, the culturing room of ventilation cultivate; Can turn out preferred kind of ripe mite after 6-10 days;
2, anti-famine is selected excellent
The pupa or the alternative host of grub that put into larva of bee or silkworm in Pu mite incubator are stand-by.The kind mite of buying is taken out from refrigerating box, be placed on temperature 20-30 ℃, in the cleaning of relative moisture 55%-80%, the culturing room of ventilation, placed 15-30 days, insert the above-mentioned Pu mite incubator that substitutes the host of having put into die, stay the Pu mite of strong, anti-famine Deng the weak Pu mite of a part after, be placed on temperature 20-30 ℃, the cleaning of relative moisture 55%-80%, the culturing room of ventilation cultivate, and can turn out after 6-10 days and select excellent anti-hungry kind mite.
3, cold-resistant select excellent
The pupa or the grub of putting into larva of bee or silkworm in Pu mite incubator substitute the host, the kind mite of buying is taken out from refrigerating box in the above-mentioned Pu mite incubator of access, be placed on temperature 18-22 ℃, the cleaning of relative moisture 55%-80%, the culturing room of ventilation cultivate, and can turn out after 15-25 days and select cold-resistant kind of excellent mite.
Four, breed the Pu mite
1, doubly amount inoculation
The pupa of mature larva of the honeybee that collects or silkworm or grub are substituted the host put into plastic culture dish, put into the mature larva of 30-40 honeybee in each in plastic culture dish or the 10-20 mulberry silkworm chrysalis put into by the foster ware of each plastics or each plastic culture dish is put into 30-40 grub.Planting in the interior plastic culture dish of mite access through preferred, the ratio of the mature larva of Pu mite and honeybee, mulberry silkworm chrysalis, grub is respectively 6000--10000: 1,10000--20000: 1,6000--10000: 1.
2, cultivate
The Pu mite incubator that is placed with the alternative host who connects kind of mite is put into temperature 20-30 ℃, and the cleaning of relative moisture 55%-80%, the culturing room of ventilation cultivate.After 2-6 days, Deng the pupa of the larva of honeybee or silkworm or grub substitute the host on one's body parasitism expired the Pu mite, and when the Pu mite begins fixedly mostly, above-mentioned alternative host is moved on in the test tube, each test tube can be put larva or 2-4 mulberry silkworm chrysalis or 2-10 grub of 2-10 honeybee, clog the mouth of pipe of test tube then with cotton, be placed on 20 ℃-30 ℃ of temperature, the cleaning of relative moisture 55%-80%, the culturing room of ventilation cultivate.Can turn out ripe Pu mite after 6-12 days.
3, the preservation of Pu mite
The test tube that cultured Pu mite is housed is placed on earlier in the temporary room of 17 ℃-20 ℃ of temperature, relative moisture 55%-70% preserved 24 hours to 48 hours, and then put into-5-15 ℃, relative moisture is preserved in the 55%-70% finished room.
In order to raise the efficiency, can cultivate the alternative host of Pu mite, preferably breed mature larva and the grub of honeybee specially, mature larva of honeybee and the mating system of grub are described respectively below.
I, directed numerous honeybee
If general bee-keeping householder produces products such as honey, royal jelly, pollen, so need a large amount of workers bee.And the build of worker bee than drone want half as large more than, the Pu mite amount of breeding is also few over half, 1 worker bee can breed out 200,000 Pu mites, 1 drone then can breed out 400,000 Pu mites.Therefore, adopt the mode of directed numerous honeybee to breed the larva of honeybee, production drone as much as possible.In numerous honeybee season, one side is put the septum of a worker bee on each frame, and another side is put the septum of a drone, impels the ovum of voluminous drone on the honeybee, with the mature larvas of many production drones.
II, breed grub
Grub is the general name of chafer larva.At first prepare the farmland, interplanting pears or apple, soybean and common vegetable.Every kind 1 capable pear or apple kind 10 row soybean and 5 row vegetables, 40 centimetres of soybean line spaces, the vegetables line space is looked different cultivars and difference.Be 50 centimetres between pears or apple, soybean, vegetables apart, and between them, stamp ridge.Lure chafer with common black light lamp, various chafers all can, but to lure spot beak rutelian (Adoretus tenuimaculatus Waterhouse).In Shanxi, the chafer that lure general mid-May is spot beak rutelian mostly.Grub black light lamp is set with breeding, establishes 1-2, open black light lamp every night in mid-May-early June and lure for every mu.In mid or late June and in the scope of 15 centimetres of soil layers, gather the larva grub of chafer September.
For easy to operate, the present invention has prepared Pu mite culturing rack.Pu mite culturing rack is for the abundant parasitism that guarantees the host and fetches alternative host in process of production more easily, can enhance productivity.When preparing the alternative host of Pu mite, alternative host is placed in the Pu mite culturing rack, being housed, the culturing rack that substitutes the host puts into plastic culture dish, and in Pu mite reproductive process, inoculate, breed and discharge.Pu mite culturing rack is made of plastic, is tubular, and bottom is a round bottom, and the top is a circle, and circle and round bottom are fused by three perpendicular slices.Each Pu mite culturing rack is put into an alternative host during use, different according to the different size that substitutes the host, the Pu mite culturing rack that larva of bee, grub are used is of a size of: the Pu mite culturing rack that diameter 6--8mm, high 8--10mm, mulberry silkworm chrysalis use is of a size of: diameter 11mm, high 30mm.
Five, the transportation of Pu mite
Need grow distance when between forest, discharging the Pu mite on a large scale and transport the Pu mite.For guaranteeing long distance safe transport Pu mite in enormous quantities, improve the yield rate of Pu mite, reduce Pu mite lethality, from refrigerating box, take out and adorning the test tube of having cultivated the Pu mite, test tube is put in the breathable plastic box, place 100-200 test tube in each breathable plastic box, again plastic casing is put into the transportation incubator.The transportation incubator is provided with the low pressure refrigerator, and refrigerator directly uses 12 volts of power supplys of automobile.The temperature of transportation incubator is controlled at 10 ℃-15 ℃.Can place 500 breathable plastic boxes in each transportation incubator.
Six, use
The Pu mite is mainly used in the control of forestry moth dryness pest, can effective parasitic red turpentine beetle (Dendroctonus Valens Le Conte), anoplophora glabripennis (Anoplophoraglabripennis (Motsch.)) waits and eat into dryness forestry pest.
1, method for releasing
Prepare the Pu mite and discharge box, the Pu mite is discharged the lacing film nail of box on the host tree that is endangered by trunk borer.Open the cotton plug of test tube, cultured Pu mite is in vitro put into the Pu mite together with Pu mite culturing rack discharge box, test tube is inserted in the hole that discharges box, cotton plug is hung got final product in the tree.
2, optional the taking off of burst size stated any delivery mode
A, control formula discharge.
When adopting individual plant control formula to discharge, invade the hole by each and put into ten thousand Pu mites of 2-4.The computing formula of Pu mite burst size is: invade ten thousand/hole, hole * (2-4).
B, preventive discharge.
When adopting the individual plant preventive to discharge, thousand Pu mites of 2-4 are put in each intrusion hole.The computing formula of Pu mite burst size is: invade thousand/hole, hole * (2-4).
Pu mite mating system of the present invention, select for use larva of honeybee or pupa or the grub of silkworm to be alternative host, the honeybee particularly pupa of honeybee and silkworm is cultured in a large number in China, all can collect throughout the year, the pupa that all can collect the mature larva of honeybee and silkworm in 1 year from the May to October is the host as an alternative, this just in time matches with the control of trunk borer season, grub also can be gathered on a large scale, and grub is adopted the farmland large-scale production, and technology is simple, efficient is very high, above-mentioned alternative host is not only convenient for production, wide material sources, host source abundance, the host originates guaranteed, and scale collection in enormous quantities, a large amount of hosts of substituting can be provided in the short time, satisfy the requirement that the Pu mite breeds on the industry, highly efficient in productivity, low production cost.The present invention provides the necessary precondition condition for breeding the Pu mite on the industry, can well realize that the batch production of Pu mite is bred.And production technology is easy, can produce the natural enemy Pu mite of control forestry pest at short notice, for the control of forestry pest and the ecotope allusion quotation that conserves forests have been decided the basis.Such natural enemy is bred the needs that factory just can satisfy the forest preventing and treating trunk borer in the whole nation.The advantage that the present invention gives prominence to is to cut down a large amount of precious forests, helps the ecotope that conserves forests.With the larva of honeybee as an alternative the host to breed the Pu mite be example: the whole nation has 600,000,000 strain forests every year and is eaten into the harm of dryness forestry pest and need control at present, on average need 200,000 Pu mites by every strain tree, each larva of bee can breed out 200,000 Pu mites, be that every strain trees need a larva of bee, 600,000,000 strain trees need 600,000,000 larva of bees.Annual foster 100 hive bees in the bee-keeping family of a medium-scale, every hive bee can produce 200,000 young honeybees in 1 year, each bee-keeping family can provide 2,000 ten thousand young honeybees in 1 year, and the larva of bee of gathering 30 bee-keeping families is that raw material is produced the Pu mite, just can produce the required Pu mite of national forest trunk borer biological control.And adopt other mating systems obviously is impossible realize.Pu mite mating system production cost of the present invention is low, provides the larva of 2,000 ten thousand honeybees to calculate every year by each bee-keeping family, and 0.5 fen of every larva of bee cost less than obviously is that other any alternative hosts are incomparable, has extremely significantly price advantage.With the pupa of silkworm and grub host as an alternative, to do to substitute the host with the semanotus bifasciatus larva and compare, cost is also very low.Pu mite incubator employing water of the present invention is done and is prevented escaping agent, and technology is easy, and effect is fine, is suitable for production requirement on the industry.
Description of drawings
Fig. 1 is the process chart of this Pu mite mating system.
Fig. 2 is the vertical view of the used Pu mite incubator of the present invention.
Fig. 3 is the cutaway view along A-A line among Fig. 2, and Fig. 2 amplifies relatively.
Fig. 4 is that the Pu mite discharges box embodiment one outline drawing.
Fig. 5 is that the Pu mite discharges box embodiment two outline drawings
Fig. 6 is a Pu mite culturing rack outline drawing, in kind relatively the amplification.
Fig. 7 is Pu mite culturing rack user mode figure.
Among the above-mentioned figure:
1, prepare Pu mite 2, gather host 3, the mature larva 4 of field bee, gather the pupa 5 of silkworm, gather grub 6, directed numerous honeybee 7, breed grub 8, feed chamber constant temperature refrigeration 9, preferred kind mite 10, doubly the amount inoculation 11, culturing room's constant temperature culture 12, culturing room's test tube cultivates 13, temporary room constant temperature refrigeration 14, finished room constant temperature refrigeration 15, constant temperature transportation 16, discharge natural enemy 17, interior plastic culture dish 18, outer plastic culture dish 19, stainless steel culture plate 20, water 21, hole 22, hole 23, funnel discharges box 24, liberation port 25, lacing film 26, through hole 27, insertion hole 28, test tube mouth 29, finger type test tube 30, rectangle liberation port 31, lacing film 32, through hole 33, rectangle discharges box 34, insertion hole 35, circle 36, perpendicular slice 37, round bottom 38, Pu mite culturing rack 39, mulberry silkworm chrysalis
Embodiment
Describe the embodiment of this Pu mite mating system and Pu mite incubator thereof below in detail according to embodiment and accompanying drawing thereof, but the specific embodiment of the present invention is not limited to following embodiment.
Pu mite incubator embodiment
The Pu mite incubator that Fig. 2 and Fig. 3 describe is made of 15 interior plastic culture dish 17 and 15 outer plastic culture dish 18 and a stainless steel culture plate 19.Interior plastic culture dish 17 diameters are 9 centimetres, and height is 8 millimeters.Outer plastic culture dish 18 diameters are 12 centimetres, and height is 1 centimetre, and outer plastic culture dish 18 sidewalls have 8 through holes such as hole 21 by the position of 1 millimeter of bottom.The length of stainless steel culture plate 19 is respectively 62 centimetres, 38 centimetres and 2 centimetres, and the bottom is water-tight.
Interior plastic culture dish 17 stick with glue outer plastic culture dish 18 in the middle of, again 15 outer plastic culture dish 18 are put into stainless steel culture plate 19, a stainless steel culture plate 19 is put 15 outer plastic culture dish 18, adds clear water in stainless steel culture plate 19, and the height of clear water is 6 millimeters.
Interior plastic culture dish 17 among Fig. 2 and Fig. 3, plastic culture dish 18 equal useable glass materials are made, also can be all made of plastic, and stainless steel culture plate 19 can be made with plastics or enamel.
The Pu mite discharges box embodiment one
It is infundibulate that Pu mite among Fig. 4 discharges box, and the funnel mouth that discharges box 23 at funnel has liberation port 24 and lacing film 25, at lacing film 25 through hole 26 is arranged, and in hopper base insertion hole 27 is arranged.On trunk, the liberation port 24 that funnel is discharged box 23 is buckled in trunk, hangs on the nail the nail nail.Finger type test tube 29 inserts insertion hole 27, refers to that type test tube mouth 28 is tilted to down, refers to that type test tube mouth 28 is inserted into funnel and discharges in the box 23.The diameter of the insertion hole 27 of present embodiment be 8 millimeters this.
The Pu mite discharges box embodiment two
It is rectangle that Pu mite among Fig. 5 discharges box, discharging box 33 in rectangle has rectangle liberation port 30 and lacing film 31 and through hole 32, at 33 ends of rectangular release box insertion hole 34 is arranged, and refers to that type test tube 29 inserts insertion hole 34, finger type test tube mouth 28 is tilted to down, refers to that type test tube mouth 28 is inserted in the release box 33.The diameter of the insertion hole 34 of present embodiment is 8 millimeters.
Pu mite culturing rack embodiment
The Pu mite culturing rack of present embodiment is made of plastic, sees Fig. 6, Fig. 7, is tubular, and bottom is a round bottom 37, and the top is a circle 35, and circle 35 and round bottom 37 are fused by three perpendicular slices 36.Present embodiment is that the Pu mite culturing rack 38 of mulberry silkworm chrysalis 39 is of a size of: diameter 12mm, high 30mm.
Mating system embodiment one
Its steps in sequence is as follows:
One, prepares the Pu mite
The 4--5 month, buy the Pu mite developed into full swollen abdomen body in the forestry PCO phase, be put in-preserve in the refrigerating box of 5-15 ℃, select to plant a mite and prepare for carrying out orientation.
Two, prepare the alternative host of Pu mite
Present embodiment is selected the alternative host of the mature larva of honeybee as breeding Pu mite for use,
1, prepares Pu mite incubator, the Pu mite incubator that promptly above-mentioned Pu mite incubator embodiment describes.
Plastic culture dish put 15 pairs in each stainless steel culture plate inside and outside adds clear water in stainless steel culture plate 19, the height of clear water is 6 millimeters.
2, prepare Pu mite culturing rack, the Pu mite culturing rack that promptly above-mentioned Pu mite culturing rack embodiment describes.
Alternative host is placed on inoculation in the Pu mite culturing rack 38, breeds and discharges.
3, directed numerous honeybee
In beehive, one side is put the septum of a worker bee on each frame, and another side is put the septum of a drone, and in time takes the mature larva of the drone of firm capping away, promotes the ovum of queen bee fecund drone, with the mature larva of many production drones.
4, the preparation of the mature larva of honeybee
Open beehive, the honeybee spleen of selecting to have developed into mature larva mostly takes out together with the honeybee frame, takes out mature larva gently with tweezers and put into Pu mite culturing rack from honeycomb, and Pu mite culturing rack is put into plastic culture dish.The honeycomb that has sealed La Gai can remove La Gai with tweezers earlier and take out mature larva more gently.Put into the mature larva of 40 honeybees in each in the plastic culture dish, put into temperature 10-15 ℃, relative moisture is preserved stand-by in the feed chamber of 55%-70%.
Three, select excellent kind of mite
Can select excellent, anti-famine to select with the power of creeping according to different purposes and excellent, cold-resistantly select excellent three kinds of preferred any methods preferably to plant mite, employing also capable of being combined be selected excellent.Present embodiment is to adopt the power of creeping to select excellent.
The Pu mite of buying is placed on 50 millimeters of diameters, long 1500 millimeters a end of lucite tube, and the other end is put into the larva of bee of putting into Pu mite culturing rack, and the ratio of the larva of Pu mite and honeybee is 10000: 1.The two ends of lucite tube are clogged with rubber stopper, prevent that the Pu mite from runing away.Again lucite tube is placed on 20 ℃-30 ℃ of temperature, the culturing room of relative moisture 55%-80%, cleaning, ventilation cultivates.After 10 days, colonize in the mature larva of honeybee and after the surface that substitutes the host is fixing Deng the Pu mite of lucite tube one end, the larva of the alternative host honeybee of having fixed the Pu mite is on one's body put into test tube together with Pu mite culturing rack, put into the larva of 5 honeybees in each test tube, again test tube is placed on temperature 20-30 ℃, the cleaning of relative moisture 55%-80%, the culturing room of ventilation cultivate.Can turn out the ripe excellent kind of mite that select after 8 days.
Four, breed the Pu mite
1, doubly amount inoculation
The mature larva of the honeybee that collects is put into Pu mite culturing rack, and Pu mite culturing rack is put into plastic culture dish, puts into the mature larva of 40 honeybees in each in the plastic culture dish.Putting into Pu mite incubator through preferred kind mite, the ratio of the mature larva of Pu mite and honeybee is 8000: 1.
2, cultivate
The Pu mite incubator that connects kind of mite is put into temperature 20-30 ℃, and the cleaning of relative moisture 55%-80%, the culturing room of ventilation cultivate.After 3 days, expired the Pu mite Deng parasitism on the larva body of honeybee, and when the Pu mite begins fixedly mostly, the larva that substitutes the host honeybee is moved on in the test tube together with Pu mite culturing rack, each test tube can be put 5 larva of bees, clog the mouth of pipe of test tube then with cotton, be placed on 20 ℃-30 ℃ of temperature, the cleaning of relative moisture 55%-80%, the culturing room of ventilation cultivate.Can turn out ripe second generation Pu mite after 8 days, every larva of bee on average can breed out 200,000 Pu mites.
3, the preservation of Pu mite
Preserved 30 hours the temporary room that the test tube of having cultivated the Pu mite is placed on 17 ℃-20 ℃ of temperature, relative moisture 55%-70% earlier is housed, and then put into temperature and be-5-15 ℃, the finished room of relative moisture 55%-70% is preserved.
Five, the transportation of Pu mite
Take out from refrigerating box and adorning the test tube of having cultivated the Pu mite, test tube is put into the breathable plastic box, promptly have the hamper that has of ventilation hole, put into 100 test tubes in each breathable plastic box, test tube keeps flat, and again plastic casing is put into the transportation incubator.The transportation incubator is provided with the low pressure refrigerator, and refrigerator directly uses 12 volts of power supplys of automobile.The temperature of transportation incubator is controlled at 10 ℃-15 ℃.Can place 500 breathable plastic boxes in each transportation incubator.
Six, use
The Pu mite that present embodiment is cultivated is applied to the anti-smelting of forestry pest red turpentine beetle.
1, method for releasing
Prepare the Pu mite and discharge box, the end that the Pu mite discharges box has a lacing film, and the Pu mite is discharged the lacing film nail of box on the host tree that is endangered by trunk borer.Open the cotton plug of test tube, cultured Pu mite is in vitro poured into discharge in the box, test tube is inserted in the hole that discharges box, cotton plug is hung in the tree.Present embodiment adopts funnel to discharge box 23.
2, burst size
Present embodiment adopts individual plant control formula to discharge, and invades the hole by each and discharges 20,000 Pu mites.The computing formula of Pu mite burst size is: invade ten thousand/hole of hole * 2-4.
Present embodiment is prepared 50,000 pairs of inside and outside plastic culture dish altogether, 3500 of stainless steel culture plates, and 2,000 ten thousand in test tube, is bred each 8 of chamber, finished rooms at feed chamber.The Pu mite discharges 2,000 ten thousand in box, and 2,000 ten thousand of the mature larvas of field bee are bred about 4,000,000,000 of Pu mite.Can prevent 2,000,000 mu of forestry pests of smelting--red turpentine beetle.
Mating system embodiment two
Its steps in sequence is as follows:
One, prepares the Pu mite
Silkworm in spring, summer silkworm the or when autumn, silkworm developed into mature larva, buy the Pu mite that has developed into full swollen abdomen body in market, put into-refrigerating box of 5-15 ℃ preserves, prepares for carrying out an orientation selection kind mite.
Two, prepare the alternative host of Pu mite
Present embodiment is selected the alternative host of the pupa of silkworm as breeding Pu mite for use
1, prepares Pu mite incubator and Pu mite culturing rack.
2, the preparation of mulberry silkworm chrysalis
Select just to have told silk, the mulberry cocoon that the cocoon shell has just been pupated is firmly put into temperature 10-15 ℃, and relative moisture is preserved stand-by in the feed chamber of 55%-70%.During inoculation, cut off the mulberry cocoon with scissors, mulberry silkworm chrysalis is put into Pu mite culturing rack, and Pu mite culturing rack is put into plastic culture dish.Putting into 20 mulberry silkworm chrysalis in the plastic culture dish in each waits to inoculate.
Three, select excellent kind of mite
Can select excellent, anti-famine to select with the power of creeping according to different purposes excellent, cold-resistantly selects excellent three kinds of preferred any methods preferably to plant mite, also can compound employing select excellent.It is excellent that present embodiment adopts anti-famine to select, and it is stand-by to put into the mulberry silkworm chrysalis of putting into Pu mite culturing rack in interior plastic culture dish.The kind mite of buying is taken out from refrigerating box, be placed on temperature 20-30 ℃, in the cleaning of relative moisture 55%-80%, the culturing room of ventilation, placed approximately 15 days, after death stay the Pu mite incubator that inserts the mulberry silkworm chrysalis of having put into Pu mite culturing rack behind the Pu mite of strong, anti-famine Deng the weak Pu mite of a part, be placed on temperature 20-30 ℃, the cleaning of relative moisture 55%-80%, the culturing room of ventilation cultivate, and can turn out after 8 days and select excellent anti-hungry kind mite.The employing power of creeping is selected when excellent, and each in vitro puts into 4 mulberry silkworm chrysalis.
Four, breed the Pu mite
1, doubly amount inoculation
Basic identical with mating system embodiment one, difference is: the ratio of Pu mite and mulberry silkworm chrysalis is 10000: 1.
2, cultivate
Basic identical with mating system embodiment one, difference is: each mulberry silkworm chrysalis on average can be bred out 100,000 Pu mites.
3, the preservation of Pu mite is identical with mating system embodiment one.
Five, the transportation transportation resources of Pu mite is basic identical with mating system embodiment one.
Six, the Pu mite of application present embodiment cultivation is applied to the anti-smelting of forest moth dryness pest.
1, method for releasing is identical with mating system embodiment one.
2, burst size is identical with mating system embodiment one.
Present embodiment is prepared 100,000 pairs of inside and outside plastic culture dish altogether, 5000 of stainless steel culture plates, and 2,000 ten thousand in test tube, is bred each 8 of chamber, finished rooms at feed chamber.The Pu mite discharges 2,000 ten thousand in box, gathers 2,000 ten thousand of mulberry silkworm chrysalis, breeds about 2,000,000,000 of Pu mite.Can prevent 1,000,000 mu of forestry pests of smelting anoplophora glabripennis.
Mating system embodiment three
Its steps in sequence is as follows:
One, it is identical with mating system embodiment one that preparation Pu mite is prepared the Pu mite.
Two, prepare the alternative host of Pu mite
Present embodiment is selected the alternative host of grub as breeding Pu mite for use,
1, prepares Pu mite incubator and Pu mite culturing rack
2, the preparation of grub
The grub of gathering in soil layer 15 cm range is collected, remove soil and weeds, be placed in the Pu mite culturing rack, Pu mite culturing rack is put into plastic culture dish, puts into temperature 10-15 ℃, and relative moisture is preserved stand-by in the feed chamber of 55%-70%.
Three, select excellent kind of mite
Can select excellent, anti-famine to select with the power of creeping according to different purposes excellent, cold-resistantly selects excellent three kinds of preferred any methods preferably to plant mite, also can compound employing select excellent.Present embodiment be adopt cold-resistant select excellent, in Pu mite incubator, put into the grub of putting into Pu mite culturing rack, the kind mite of buying is taken out from refrigerating box in the above-mentioned Pu mite incubator of access, be placed on temperature 18-22 ℃, the cleaning of relative moisture 55%-80%, the culturing room of ventilation cultivate, and can turn out after 20 days and select cold-resistant kind of excellent mite.
Four, breed the Pu mite
1, doubly the amount inoculation is identical with mating system embodiment one.
2, cultivation is identical with mating system embodiment one, and each grub on average can breed out 200,000 Pu mites.
3, the preservation of Pu mite is identical with mating system embodiment one.
Five, the transportation transportation resources of Pu mite is identical with mating system embodiment one.
Six, use
The Pu mite that present embodiment is cultivated is applied to the anti-smelting of forest moth dryness pest.
1, method for releasing is identical with mating system embodiment one.
2, burst size is identical with mating system embodiment one.
Present embodiment is prepared 50,000 pairs of inside and outside plastic culture dish altogether, 3500 of stainless steel culture plates, and 2,000 ten thousand in test tube, is bred each 8 of chamber, finished rooms at feed chamber.The Pu mite discharges 2,000 ten thousand in box, and 2,000 ten thousand of the mature larvas of field bee are bred about 4,000,000,000 of Pu mite.Can prevent 1,000,000 mu of forestry pests of smelting red turpentine beetle.

Claims (5)

1. Pu mite mating system, its mating system comprises following steps successively:
One, prepares the Pu mite
The 4--5 month, buy fully-developed Pu mite in the forestry PCO phase, be put in-preserve in the refrigerating box of 5-15 ℃;
Two, prepare the alternative host of Pu mite
(1), prepares Pu mite incubator
Pu mite incubator is by a stainless steel culture plate and some plastic culture dish is formed, every pair of plastic culture dish is made up of inside and outside two plastic culture dish, the interior plastic culture dish of 9 centimetres of diameters is bonded at glue in the middle of the outer plastic culture dish of 12 centimetres of diameters, the height of outer plastic culture dish is a little more than interior plastic culture dish, and the plastic culture dish sidewall has the through hole of 2 millimeters on a circle by the position of 1 millimeter of bottom outside; The interior plastic culture dish that bonds together is put into the watertight stainless steel culture plate in bottom with outer plastic culture dish;
(2), prepare to substitute the host with following any method
The preparation of the mature larva of a, honeybee
Open beehive, the honeybee spleen of selecting to have developed into mature larva mostly takes out together with the honeybee frame, from honeycomb, take out mature larva and put into plastic culture dish with tweezers, plastic culture dish is put into the mature larva of 30-40 honeybee in each, put into temperature 10-15 ℃, relative moisture is preserved in the feed chamber of 55%-70%;
The preparation of b, mulberry silkworm chrysalis
Select just to have told silk, the mulberry cocoon that the cocoon shell is not pupated is firmly as yet put into temperature 10-15 ℃, and relative moisture is preserved in the feed chamber of 55%-70%; During inoculation, cut off the mulberry cocoon, take out mulberry silkworm chrysalis, put into plastic culture dish with scissors; Putting into 10-20 mulberry silkworm chrysalis in the plastic culture dish in each waits to inoculate; Put into temperature 10-15 ℃ again, relative moisture is preserved in the feed chamber of 55%-70%;
The preparation of C, grub
The grub of gathering in soil layer 15 cm range is collected, put into plastic culture dish after removing soil and weeds, put into 30-40 grub in plastic culture dish in each, put into temperature 10-15 ℃ again, relative moisture is preserved in the feed chamber of 55%-70%;
Three, select excellent kind of mite
Select excellent method preferably to plant mite with following any
(1), the power of creeping is selected excellent
The Pu mite of buying is placed on an end of the lucite tube of 30 millimeters-50 millimeters of diameters, 1000 millimeters-1500 millimeters of length, pupa or grub that the other end is put into larva of bee or silkworm substitute the host, and the ratio that larva of Pu mite and honeybee or the pupa of silkworm or grub substitute the host is 10000--20000: 1; The two ends of lucite tube are clogged with rubber stopper, again lucite tube is placed on 20 ℃-30 ℃ of temperature, the culturing room of relative moisture 55%-80%, cleaning, ventilation cultivates; After 10-18 days, the Pu mite of lucite tube one end colonizes in the pupa of the mature larva of honeybee or silkworm or grub substitutes on the pin main body and after the surface that substitutes the host is fixing, above-mentioned alternative host is put into test tube, each test tube is put into larva or 2-5 mulberry silkworm chrysalis or 2-10 grub of 2-10 honeybee, again test tube is placed on temperature 20-30 ℃, the cleaning of relative moisture 55%-80%, the culturing room of ventilation cultivate; Can turn out preferred kind of ripe mite after 6-10 days;
(2), anti-famine is selected excellent
The pupa or the alternative host of grub that put into larva of bee or silkworm in Pu mite incubator are stand-by; The kind mite of buying is taken out from refrigerating box, be placed on temperature 20-30 ℃, the cleaning of relative moisture 55%-80%, the culturing room of ventilation, placed 15-30 days, die, stay Deng the weak Pu mite of a part and insert the larva of putting honeybee well behind the Pu mite of strong, anti-famine or pupa or the grub of silkworm substitutes in host's the interior plastic culture dish, be placed on temperature 20-30 ℃, the cleaning of relative moisture 55%-80%, the culturing room of ventilation cultivate, and can turn out after 6-10 days and select excellent anti-hungry kind mite;
(3), cold-resistant select excellent
The pupa or the grub of putting into larva of bee or silkworm in Pu mite incubator substitute the host, the kind mite of buying is taken out from refrigerating box in the interior plastic culture dish of access, be placed on temperature 18-22 ℃, the cleaning of relative moisture 55%-80%, the culturing room of ventilation cultivate, and can turn out after 15-25 days and select cold-resistant kind of excellent mite;
Four, breed the Pu mite
(1), doubly amount inoculation
The pupa of mature larva of the honeybee that collects or silkworm or grub are substituted the host put into plastic culture dish, put into the mature larva of 30-40 honeybee in each interior plastic culture dish or each plastic culture dish is put into 10-20 mulberry silkworm chrysalis or each plastic culture dish is put into 30-40 grub; Selecting excellent kind mite to insert in the interior plastic culture dish, the ratio of the mature larva of Pu mite and honeybee, mulberry silkworm chrysalis, grub is respectively 6000--10000: 1,10000--20000: 1,6000--10000: 1;
(2), cultivate
The Pu mite incubator that is placed with the alternative host who connects kind of mite is put into temperature 20-30 ℃, the cleaning of relative moisture 55%-80%, the culturing room of ventilating cultivated 2-5 days, Deng the pupa of the larva of honeybee or silkworm or grub substitute the host on one's body parasitism expired the Pu mite, and when the Pu mite begins fixedly mostly, pupa of larva that substitutes the host honeybee or silkworm or the alternative host of grub are moved on in the test tube, each test tube can be put the larva of 2-10 honeybee, or 2-4 mulberry silkworm chrysalis, or 2-10 grub, clog the mouth of pipe of test tube then with cotton, be placed on 20 ℃-30 ℃ of temperature, the cleaning of relative moisture 55%-80%, the culturing room of ventilating cultivated 6-12 days, can turn out ripe Pu mite.
2. Pu mite mating system according to claim 1 is characterized in that: when preparing the alternative host of Pu mite, and directed numerous honeybee earlier, in numerous honeybee season, one side is put the septum of a worker bee on each frame, and another side is put the septum of a drone.
3. Pu mite mating system according to claim 1 is characterized in that: when preparing the alternative host of Pu mite, breed grub earlier; In the farmland, interplanting pears or apple, soybean and common vegetable; Every kind 1 capable pear or apple kind 10 row soybean and 5 row vegetables, 40 centimetres of soybean line spaces are 50 centimetres apart between pears or apple, soybean, vegetables, and stamp ridge between them; Lure chafer with common black light lamp, to lure spot beak rutelian; Grub black light lamp is set with breeding, establishes 1-2 for every mu, annual in mid or late June and in the scope of 15 centimetres of soil layers, gather the larva grub of chafer September.
4. according to claim 1 or 2 or 3 described Pu mite mating systems, it is characterized in that: when preparing the alternative host of Pu mite, alternative host is placed in the Pu mite culturing rack, puts into plastic culture dish the culturing rack that substitutes the host is housed, in Pu mite reproductive process, inoculate, breed; Pu mite culturing rack is made of plastic, is tubular, and bottom is a round bottom, and the top is a circle, and circle and round bottom are fused by three perpendicular slices; Each Pu mite culturing rack is put into an alternative host.
5. Pu mite incubator, it is by a stainless steel culture plate and some plastic culture dish is formed, every pair of plastic culture dish is made up of inside and outside two plastic culture dish, the interior plastic culture dish of 9 centimetres of diameters is bonded at glue in the middle of the outer plastic culture dish of 12 centimetres of diameters, the height of outer plastic culture dish is a little more than interior plastic culture dish, and the plastic culture dish sidewall has the through hole of 2 millimeters on a circle by the position of 1 millimeter of bottom outside; The interior plastic culture dish that bonds together is put into the watertight stainless steel culture plate in bottom with outer plastic culture dish.
CNB2005100128538A 2005-09-23 2005-09-23 Method for breeding pyemote, and its culturing vessel, and method for releasing same Expired - Fee Related CN100388884C (en)

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