CN108125074B - Artificial feed for athetis lepigone and feeding method thereof - Google Patents

Artificial feed for athetis lepigone and feeding method thereof Download PDF

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CN108125074B
CN108125074B CN201810209809.3A CN201810209809A CN108125074B CN 108125074 B CN108125074 B CN 108125074B CN 201810209809 A CN201810209809 A CN 201810209809A CN 108125074 B CN108125074 B CN 108125074B
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athetis lepigone
feed
distilled water
adult
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CN108125074A (en
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田彩红
封洪强
张佳佳
黄建荣
李国平
邱峰
封洪云
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Institute of Plant Protection of Henan Academy of Agricultural Sciences
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    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
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    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
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    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
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    • A23L11/30—Removing undesirable substances, e.g. bitter substances
    • A23L11/31—Removing undesirable substances, e.g. bitter substances by heating without chemical treatment, e.g. steam treatment, cooking
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Abstract

The invention belongs to the field of plant protection, and particularly discloses an artificial feed for athetis lepigone, which comprises a larva feed and an adult supplementary nutrient solution, wherein the larva feed comprises the following raw materials in parts by weight: 200-300 parts of corn flour, 110-150 parts of soybean flour, 80-100 parts of wheat germ powder, 20-35 parts of yeast powder, 3-15 parts of potassium sorbate, 5-15 parts of agar, 3-5 parts of vitamin, 3-10 parts of edible vegetable oil and 1000-1500 parts of distilled water; also provides a simple feeding method of the artificial feed. The invention has the advantages of easily obtained raw materials, low cost, convenient preparation, long storage time, comprehensive nutrient components, stable feeding effect and strong practicability, is beneficial to feeding athetis lepigone on a large scale, and ensures that the feeding process becomes reliable, simple, efficient and environment-friendly.

Description

Artificial feed for athetis lepigone and feeding method thereof
Technical Field
The invention belongs to the field of plant protection, and particularly relates to an agricultural pest-athetis lepigone artificial feed and a simple feeding method thereof.
Background
Athetis lepigoneAthetis dissimilis(Hampson) belongs to Lepidoptera, noctuidae and Venetia committee, mainly harms crops such as corn, vegetables, wheat, soybean, peanut, sweet potato and traditional Chinese medicines, is often harmed by being mixed with Athetis lepigone and has the food intake far larger than that of Athetis lepigone, and is an important pest newly generated in Huang-Huai-Hai summer corn fields after the transformation of the farming system (no-tillage after wheat harvest and iron stubble sowing) in China. The athetis lepigone mainly gnaws the base of the corn stem and the aerial root of the new born, forms a cavity, causes the heart leaves of the overground part to wither and die, and causes the plant to fall down if the soil is serious. In summer maize production areas, larvae often hide in broken wheat straws around maize seedlings and under dry branches and leaves of field crops, and have the habit of concealing harm, and once large areas occur, seedling shortage and ridge breaking are often caused. Therefore, the prevention and the treatment of the athetis lepigone seedling protection and the production preservation are urgent.
The research of insect artificial feed has been in the history of hundreds of years, and at present, the artificial feed reported at home and abroad has more than 1000 kinds, and the variety of the fed insects is nearly 900 kinds. With the development of pest comprehensive control (IPM), a large number of insects are needed in the researches of insect physiology, toxicology, ecology, pathology, microbial insecticide, radiation sterility, chemical sterility, attractant, pheromone and the like, and the insects are fed with artificial feed, so that the insects are consistent in age and good in development index, and the work can be better met. The athetis lepigone is an agricultural pest newly found in recent years, the adult is active, the larva activity is hidden, the adult is difficult to find in the field, the artificial control is difficult, the natural feed feeding is easy to pollute, and the annual feeding is difficult. Therefore, the mass propagation of the athetis lepigone not only provides test materials for biological toxicity determination, but also provides an insect source foundation for the development of biological pesticides, lays a foundation for further research on the biology, toxicology and genetics of the athetis lepigone, and urgently needs to develop artificial feed and a set of simple and rapid breeding technology for the athetis lepigone.
Disclosure of Invention
Aiming at the problems, the invention aims to provide an artificial feed formula for athetis lepigone with strong practicability and a simple and rapid feeding technology.
In order to achieve the purpose, the invention is realized by the following technical scheme:
the artificial feed for the athetis lepigone comprises a feed for larvae of the athetis lepigone, wherein the feed for the larvae of the athetis lepigone comprises the following raw materials in parts by weight: 200-300 parts of corn flour, 110-150 parts of soybean flour, 80-100 parts of wheat germ powder, 20-35 parts of yeast powder, 3-15 parts of potassium sorbate, 5-15 parts of agar, 3-5 parts of vitamin, 3-10 parts of edible vegetable oil and 1000-1500 parts of distilled water.
The Athetis lepigone larva feed preferably comprises the following raw materials in parts by weight: 220-225 parts of corn flour, 110-120 parts of soybean flour, 85-95 parts of wheat germ powder, 25-30 parts of yeast powder, 5-10 parts of potassium sorbate, 5-10 parts of agar, 3-4 parts of vitamin, 3-8 parts of edible vegetable oil and 1000-1200 parts of distilled water.
The edible vegetable oil is preferably corn germ oil; the distilled water is preferably sterilized distilled water; the vitamin is preferably vitamin H.
The Athetis lepigone larva feed is prepared by the following steps:
(1) heating the soybean meal for 5-10 minutes by small fire in a microwave oven (roasting aroma), uniformly stirring the soybean meal, the wheat germ meal, the corn meal and half of distilled water (placing the mixture in a plastic container with scales and using a loose hand-held electric stirrer), heating the mixture for 6-8 minutes by high fire in the microwave oven, and uniformly stirring the mixture again to obtain a component A;
(2) mixing the agar and the balance of distilled water (placing the mixture in another plastic container with scales and using a loose hand-held electric stirrer) and stirring uniformly, then placing the mixture in a microwave oven and heating the mixture for 6 to 8 minutes by high fire, and then stirring uniformly again to obtain a component B;
(3) placing the obtained component A in a microwave oven for further high-fire heating for 1-3 minutes, and then uniformly stirring again;
(4) placing the obtained component B in a microwave oven for further heating for 1-3 minutes by high fire (taking care not to boil and splash out of the container in the heating process);
(5) adding the obtained component A into the component B, then adding the edible vegetable oil, stirring, simultaneously adding the yeast powder, the vitamins and the potassium sorbate, uniformly stirring to obtain the athetis lepigone larva feed (preferably, distilled water is added to a constant volume of 1600 parts by volume, and the feed is further uniformly stirred, wherein the unit g of the weight part corresponds to mL of the volume part), cooling to 55-65 ℃ for later use, or storing in a refrigerator at 4 ℃.
The stirring tool is a sterilized stainless steel hand-held stirrer.
The artificial feed for the athetis lepigone also comprises a nutrient solution for supplementing adult athetis lepigone, wherein the nutrient solution for supplementing adult athetis lepigone comprises the following raw materials in parts by weight: 30-40 parts of cane sugar, 30-40 parts of honey, 0.1-0.5 part of sorbic acid, 0.2-0.6 part of ascorbic acid and 1000-1500 parts of sterilized distilled water.
The adult athetis lepigone supplementary nutrient solution is prepared by the following steps: heating the cane sugar, the honey, the sorbic acid and the sterilized distilled water in a microwave oven for 4-6 minutes by high fire, then cooling to 56-65 ℃, adding the ascorbic acid, uniformly mixing, and cooling to room temperature to obtain the honey-sugar-free water-based composite material.
The small fire heating is to set the output power of the microwave oven to be 100-150W; the high-fire heating is to set the output power of the microwave oven to 700-800W.
The invention also provides a method for feeding athetis lepigone by using the artificial feed for the athetis lepigone, which comprises the following steps:
(1) preparing a larva feed tray: injecting the athetis lepigone larva feed (filled into a sterilized squeeze bottle) into a disposable feed tray (the depth of a hole is 1/3-2/3 cm, the diameter of the bottom of the hole is 1-3 cm, and the depth of the hole is 3-5 cm); then placing the mixture on an ultra-clean workbench for sterilization (ultraviolet lamp irradiation for 15-20 minutes), and controlling the temperature to room temperature at the wind speed of 0.4-0.6 m/s;
(2) egg-first hatched larva: inoculating the primarily hatched larvae of athetis lepigone to the feed tray, connecting 3-5 heads of each hole, coating a preservative film, and punching holes on the fresh-keeping film by using pins of 0.5-0.7 mm to facilitate the breathing of insects; and then placing the mixture at the temperature of 25.5-26.5 ℃ and the photoperiod of 16L: feeding the seeds to 3-4 years old in an incubator with 8D and 70-80% of humidity;
(3) larva of 3-4 th age-pupation: and (3) moving out the larvae of the athetis lepigone in the feed tray by using a small brush pen, inoculating 1-3 insects into a new feed tray, placing the feed tray at a temperature of 25.5-26.5 ℃ and a light cycle of 16L: feeding pupation in an incubator with 8D and humidity of 70-80%;
(4) preparing a honeypot for supplementing nutrient solution to adults: subpackaging the adult athetis lepigone supplementary nutrient solution into containers, preferably (disposable) plastic bottles (with the diameter of 3-5 cm and the height of 3-8 cm, and holes on the bottles), rolling sterilized absorbent cotton into a wick shape, penetrating the wick shape into the liquid level to absorb the nutrient solution, and reserving 1-3 cm on the wick shape for the adult athetis lepigone to absorb the nutrient solution;
(5) pupal stage-adult: picking out pupae with dark brown epidermis, separating male pupae from female pupae (under a microscope), rinsing the pupae for 2-3 times, and cleaning the pupae with distilled water; then, the male and female pupae are placed into a culture dish after being paired, the culture dish and a prepared adult nutrient solution supplementing honeypot are placed into a spawning cage (a nylon gauze which is rinsed cleanly is hung in the cage to enable the female moth to spawn), and then the spawning cage is placed in a 16L photoperiod: 8D, in an incubator with the humidity of 70-80% until the third day after eclosion of the imagoes;
(6) adult-egg: collecting nylon egg cloth in the spawning cage, slightly shaking the nylon egg cloth in distilled water for 3-5 times to enable the eggs to fall off, rinsing the rinsing liquid for 1-4 times, washing the eggs with sterilized distilled water, collecting the eggs on moisture-preserving filter paper, and then placing the moisture-preserving filter paper in a self-sealing bag to wait for larvae to hatch out;
(7) and (4) after the larvae are hatched, repeating the steps 1, 2, 3, 4, 5 and 6 in sequence, and circulating to finish subculture.
Preferably, the rinsing liquid used for rinsing is an aqueous solution containing 0.12-0.16 g/L of sodium hypochlorite and 6.0-7.2 g/L of sodium chloride.
The invention has the following positive and beneficial effects:
(1) the method for preparing the athetis lepigone larva feed is quick, simple and convenient, and a technician can independently complete large-scale feed preparation work after simple training.
(2) The artificial feed for the athetis lepigone is low in price and easy to obtain raw materials, the soybean meal is roasted in a microwave oven with small fire to be fragrant, toxic substance saponin in the raw soybean meal can be converted into nutrient substances suitable for absorption, and the artificial feed has remarkable economic benefit.
(3) The potassium sorbate used in the invention is a relatively safe food preservative, has high antibacterial performance, mainly inhibits the growth and reproduction of mould, has inhibiting effect on bacteria, mould and saccharomycetes, greatly improves the capability of resisting bacteria of larvae, and greatly reduces the death rate of the larvae; the edible corn germ oil is rich in vitamin A, D, E, vitamin E complex and 80-85% of linoleic acid and other unsaturated fatty acids, and can be well absorbed by insect organisms.
(4) The main component of the vitamin used in the invention is vitamin H, which is an essential substance for synthesizing vitamin C, and is an important substance for maintaining normal metabolism of fat and protein, and can promote absorption of lepidoptera larvae.
(5) The larvae of the athetis lepigone fed by the larvae feed are fed by the bionic feed, the survival rate of the larvae is high, the life history is shortened by 5-10 days compared with that of the larvae fed by corn seedlings which are purely used as natural food, and the egg laying rate and the weight of hundred eggs of the female insects are remarkably higher than that of the larvae fed by the corn seedlings purely used.
(6) The method can be used for subculturing athetis lepigone, has strong practicability, regular development period and stable feeding effect, and provides a large amount of insect sources with consistent chemical properties for scientific research and production needs.
(7) The egg laying rate of the adult supplemental nutrient solution used by the invention is obviously improved compared with that of the prior art which uses honey or cane sugar alone. The wick-shaped device of the self-made honeypot is beneficial to more convenient absorption of adult insects, is beneficial to flying away after absorption, avoids the moth from being adhered to viscous nutrient solution, reduces the casualties of the adult insects, avoids the adult insects from spawning on objects dipped with the nutrient solution, and improves the survival rate of eggs.
Detailed Description
The present invention will be described in further detail with reference to specific examples, but the present invention is not limited to these examples.
Example 1
An artificial feed for athetis lepigone comprises an athetis lepigone larva feed and an adult athetis lepigone supplementary nutrient solution, wherein the athetis lepigone larva feed formula comprises: 200g of corn flour, 110g of soybean flour, 80g of wheat germ powder, 20g of yeast powder, 3g of potassium sorbate, 5g of agar, 3g of vitamin (vitamin H), 3g of corn germ oil and 1000mL of sterilized distilled water in parts by weight; the formula of the adult athetis lepigone supplementary nutrient solution comprises the following components: 35g of cane sugar, 35g of honey, 0.3g of sorbic acid, 0.4g of ascorbic acid and 1000mL of sterilized distilled water.
The Athetis lepigone larva feed is prepared by the following steps:
(1) heating the soybean meal in a microwave oven with soft fire for 8 minutes (roasting aroma), then uniformly stirring the soybean meal, the wheat germ meal, the corn meal and half of distilled water (placing the mixture in a plastic container with scales and using a loose hand-held electric stirrer), then placing the mixture in the microwave oven with the soft fire for heating for 7 minutes, and then uniformly stirring again to obtain a component A;
(2) mixing the agar and the balance of distilled water (placing the mixture in another plastic container with scales and using a loose hand-held electric stirrer) and stirring uniformly, then placing the mixture in a microwave oven and heating the mixture on high fire for 7 minutes, and then stirring uniformly again to obtain a component B;
(3) placing the obtained component A in a microwave oven for further heating for 2 minutes by high fire, and then uniformly stirring again;
(4) placing the obtained component B in a microwave oven and heating with high fire for 2 minutes (taking care not to boil and splash out of the container during heating);
(5) adding the obtained component A into the component B, then adding the edible vegetable oil, stirring, simultaneously adding the yeast powder, the vitamins and the potassium sorbate, uniformly stirring to obtain the athetis lepigone larva feed, adding distilled water to a constant volume of 1600mL, and cooling to 60 ℃ for later use, or storing in a refrigerator at 4 ℃.
The adult athetis lepigone supplementary nutrient solution is prepared by the following steps: heating the sucrose, the honey, the sorbic acid and the sterilized distilled water in a microwave oven with high fire for 5 minutes, then cooling to 60 ℃, adding the ascorbic acid, uniformly mixing, and cooling to room temperature to obtain the honey-sugar-free water-based composite material.
Example 2
An artificial feed for athetis lepigone comprises an athetis lepigone larva feed and an adult athetis lepigone supplementary nutrient solution, wherein the athetis lepigone larva feed formula comprises: 220g of corn flour, 115g of soybean flour, 90g of wheat germ powder, 25g of yeast powder, 6g of potassium sorbate, 8g of agar, 4g of vitamin (vitamin H), 8g of corn germ oil and 1200mL of sterilized distilled water in parts by weight; the formula of the adult athetis lepigone supplementary nutrient solution comprises the following components: 36g of sucrose, 32g of honey, 0.3g of sorbic acid, 0.4g of ascorbic acid and 1200mL of sterilized distilled water. Both were prepared in the same manner as in example 1.
Example 3
An artificial feed for athetis lepigone comprises an athetis lepigone larva feed and an adult athetis lepigone supplementary nutrient solution, wherein the athetis lepigone larva feed formula comprises: 260g of corn flour, 120g of soybean flour, 80g of wheat germ powder, 30g of yeast powder, 10g of potassium sorbate, 8g of agar, 4g of vitamin (vitamin H), 6g of corn germ oil and 1500mL of sterilized distilled water in parts by weight; the formula of the adult athetis lepigone supplementary nutrient solution comprises the following components: 40g of cane sugar, 30g of honey, 0.3g of sorbic acid, 0.4g of ascorbic acid and 1200mL of sterilized distilled water. Both were prepared in the same manner as in example 1.
Example 4
An artificial feed for athetis lepigone comprises an athetis lepigone larva feed and an adult athetis lepigone supplementary nutrient solution, wherein the athetis lepigone larva feed formula comprises: 300g of corn flour, 110g of soybean flour, 100g of wheat germ powder, 35g of yeast powder, 15g of potassium sorbate, 10g of agar, 5g of vitamin (vitamin H), 10g of corn germ oil and 1500mL of sterilized distilled water in parts by weight; the formula of the adult athetis lepigone supplementary nutrient solution comprises the following components: 38g of sucrose, 36g of honey, 0.4g of sorbic acid, 0.6g of ascorbic acid and 1200mL of sterilized distilled water. Both were prepared in the same manner as in example 1.
Example 5
An artificial feed for athetis lepigone comprises an athetis lepigone larva feed and an adult athetis lepigone supplementary nutrient solution, wherein the athetis lepigone larva feed formula comprises: 225g of corn flour, 125g of soybean flour, 90g of wheat germ powder, 25g of yeast powder, 10g of potassium sorbate, 8g of agar, 4g of vitamin (vitamin H), 6g of corn germ oil and 1200mL of sterilized distilled water in parts by weight; the formula of the adult athetis lepigone supplementary nutrient solution comprises the following components: 37g of sucrose, 30g of honey, 0.5g of sorbic acid, 0.4g of ascorbic acid and 1400mL of sterilized distilled water. Both were prepared in the same manner as in example 1.
Example 6
An artificial feed for athetis lepigone comprises an athetis lepigone larva feed and an adult athetis lepigone supplementary nutrient solution, wherein the athetis lepigone larva feed formula comprises: 220g of corn flour, 150g of soybean flour, 95g of wheat germ powder, 25g of yeast powder, 6g of potassium sorbate, 8g of agar, 3g of vitamin (vitamin H), 8g of corn germ oil and 1200mL of sterilized distilled water in parts by weight; the formula of the adult athetis lepigone supplementary nutrient solution comprises the following components: 33g of sucrose, 35g of honey, 0.3g of sorbic acid, 0.5g of ascorbic acid and 1100mL of sterilized distilled water. Both were prepared in the same manner as in example 1.
Example 7
An artificial feed for athetis lepigone comprises an athetis lepigone larva feed and an adult athetis lepigone supplementary nutrient solution, wherein the athetis lepigone larva feed formula comprises: 280g of corn flour, 130g of soybean flour, 85g of wheat germ powder, 25g of yeast powder, 8g of potassium sorbate, 12g of agar, 4g of vitamin (vitamin H), 6g of corn germ oil and 1300mL of sterilized distilled water in parts by weight; the formula of the adult athetis lepigone supplementary nutrient solution comprises the following components: 36g of sucrose, 33g of honey, 0.4g of sorbic acid, 0.6g of ascorbic acid and 1300mL of sterilized distilled water. Both were prepared in the same manner as in example 1.
Example 8
The method for feeding the athetis lepigone by using the artificial feed for the athetis lepigone in the embodiment comprises the following steps of:
(1) preparing a larva feed tray: injecting the athetis lepigone larva feed (filled into a sterilized squeeze bottle) into a disposable feed tray (until the depth of a hole is 1/3-2/3, the diameter of the bottom of the hole is 2cm, and the depth of the hole is 4 cm); then placing the mixture on an ultra-clean workbench for sterilization (ultraviolet lamp irradiation for 15-20 minutes), and controlling the temperature to room temperature at the wind speed of 0.5 m/s;
(2) egg-first hatched larva: inoculating the primarily hatched larvae of athetis lepigone to the feed tray, connecting 4 heads to each hole, coating a preservative film, and punching holes on the fresh-keeping film by using pins of 0.5-0.7 mm to facilitate the breathing of the larvae; and then placing the mixture at the temperature of 25.5-26.5 ℃ and the photoperiod of 16L: feeding the seeds to 3-4 years old in an incubator with 8D and 70-80% of humidity;
(3) larva of 3-4 th age-pupation: and (3) moving out the athetis lepigone larvae in the feed tray by using a small brush pen, inoculating the larvae onto a new feed tray, inoculating 2 larvae per hole, placing the larvae at the temperature of 25.5-26.5 ℃ and the light cycle of 16L: feeding pupation in an incubator with 8D and humidity of 70-80%;
(4) preparing a honeypot for supplementing nutrient solution to adults: subpackaging the adult athetis lepigone supplementary nutrient solution into containers, preferably (disposable) plastic bottles (with the diameter of 3cm and the height of 4cm, and the upper surface perforated), rolling the sterilized absorbent cotton into a wick shape, penetrating the wick shape into the liquid level to absorb the nutrient solution, and reserving 3cm above the wick shape for the adult athetis lepigone to absorb the nutrient solution;
(5) pupal stage-adult: picking out pupa with dark brown epidermis, separating male and female pupae (under microscope), rinsing pupae for 3 times, and cleaning with distilled water; then, the male and female pupae are placed into a culture dish after being paired, the culture dish and a prepared adult nutrient solution supplementing honeypot are placed into a spawning cage (a nylon gauze which is rinsed cleanly is hung in the cage to enable the female moth to spawn), and then the spawning cage is placed in a 16L photoperiod: 8D, in an incubator with the humidity of 70-80% until the third day after eclosion of the imagoes;
(6) adult-egg: collecting nylon egg cloth in the spawning cage, shaking gently in distilled water for 4 times to make the eggs fall off, rinsing with rinsing solution for 3 times, washing with sterilized distilled water, collecting the eggs on moisture-preserving filter paper, and placing the moisture-preserving filter paper in a self-sealing bag to allow larvae to hatch out;
(7) and (4) after the larvae are hatched, repeating the steps 1, 2, 3, 4, 5 and 6 in sequence, and circulating to finish subculture. The rinsing liquid used for rinsing is an aqueous solution containing 0.15g/L of sodium hypochlorite and 7.0g/L of sodium chloride.
The present invention is not limited to the above-described embodiments, and various changes may be made by those skilled in the art, and any changes equivalent or similar to the present invention are intended to be included within the scope of the claims.

Claims (6)

1. An artificial feed for athetis lepigone comprises an athetis lepigone larva feed and an adult athetis lepigone supplementary nutrient solution, and is characterized in that: the Athetis lepigone larva feed is prepared from the following raw materials in parts by weight: 220-225 parts of corn flour, 110-120 parts of soybean flour, 85-95 parts of wheat germ powder, 25-30 parts of yeast powder, 5-10 parts of potassium sorbate, 5-10 parts of agar, 3-4 parts of vitamin, 3-8 parts of edible vegetable oil and 1000-1200 parts of distilled water;
the edible vegetable oil is corn germ oil; the vitamin is vitamin H;
the adult athetis lepigone supplementary nutrient solution is prepared from the following raw materials in parts by weight: 30-40 parts of cane sugar, 30-40 parts of honey, 0.1-0.5 part of sorbic acid, 0.2-0.6 part of ascorbic acid and 1000-1500 parts of sterilized distilled water;
the Athetis lepigone larva feed is prepared by the following steps:
(1) heating the soybean meal in a microwave oven with soft fire for 5-10 minutes, then uniformly stirring the soybean meal, the wheat germ meal, the corn meal and half of distilled water, then heating the mixture in the microwave oven with the soft fire for 6-8 minutes, and then uniformly stirring the mixture again to obtain a component A;
(2) uniformly mixing and stirring the agar and the balance of distilled water, heating the mixture for 6-8 minutes in a microwave oven with high fire, and then uniformly stirring the mixture again to obtain a component B;
(3) placing the obtained component A in a microwave oven for further high-fire heating for 1-3 minutes, and then uniformly stirring again;
(4) placing the obtained component B in a microwave oven for further high-fire heating for 1-3 minutes;
(5) adding the obtained component A into the component B, then adding the edible vegetable oil, stirring, simultaneously adding the yeast powder, the vitamins and the potassium sorbate, uniformly stirring to obtain the athetis lepigone larva feed, and cooling to 55-65 ℃ for later use, or storing in a refrigerator at 4 ℃.
2. The artificial feed for athetis lepigone according to claim 1, wherein: and (5) adding distilled water into the obtained athetis lepigone larva feed to reach a constant volume of 1600 parts, further uniformly stirring, cooling and storing, wherein the volume part corresponds to the unit g of the weight part in unit mL.
3. The artificial feed for athetis lepigone according to claim 1, wherein: the adult athetis lepigone supplementary nutrient solution is prepared by the following steps: heating the cane sugar, the honey, the sorbic acid and the sterilized distilled water in a microwave oven for 4-6 minutes by high fire, then cooling to 56-65 ℃, adding the ascorbic acid, uniformly mixing, and cooling to room temperature to obtain the honey-sugar-free water-based composite material.
4. The artificial feed for athetis lepigone according to claim 1 or 3, wherein: the small fire heating is to set the output power of the microwave oven to be 100-150W; the high-fire heating is to set the output power of the microwave oven to 700-800W.
5. A method for rearing Athetis lepigone by using the Athetis lepigone artificial diet of claim 1, comprising the steps of:
(1) preparing a larva feed tray: injecting the athetis lepigone larva feed into a feed tray until the depth of the holes is 1/3-2/3; then placing the mixture on a sterilized ultra-clean workbench, and controlling the temperature to room temperature at the wind speed of 0.4-0.6 m/s;
(2) egg-first hatched larva: inoculating the primarily hatched larvae of athetis lepigone to the feed tray, connecting 3-5 heads of each hole, coating a preservative film, and punching holes on the fresh-keeping film by using pins of 0.5-0.7 mm to facilitate the breathing of insects; and then placing the mixture at the temperature of 25.5-26.5 ℃ and the photoperiod of 16L: feeding the seeds to 3-4 years old in an incubator with 8D and 70-80% of humidity;
(3) larva of 3-4 th age-pupation: and (3) moving out the larvae of the athetis lepigone in the feed tray by using a small brush pen, inoculating 1-3 insects into a new feed tray, placing the feed tray at a temperature of 25.5-26.5 ℃ and a light cycle of 16L: feeding pupation in an incubator with 8D and humidity of 70-80%;
(4) preparing a honeypot for supplementing nutrient solution to adults: filling the adult athetis lepigone supplementary nutrient solution into a container, rolling sterilized absorbent cotton into a wick shape, penetrating the wick shape into the liquid level to absorb the nutrient solution, and reserving 1-3 cm above the wick shape for the adult athetis lepigone to absorb the nutrient solution;
(5) pupal stage-adult: picking out pupae with dark brown epidermis, separating male pupae from female pupae, rinsing the pupae for 2-3 times, and cleaning the pupae with distilled water; then, the male and female pupae are paired and placed into a culture dish, the culture dish and prepared adult supplement nutrient solution honeypots are placed into a spawning cage, and then the spawning cage is placed in a position with a photoperiod of 16L: 8D, in an incubator with the humidity of 70-80% until the third day after eclosion of the imagoes;
(6) adult-egg: collecting nylon egg cloth in the spawning cage, slightly shaking the nylon egg cloth in distilled water for 3-5 times to enable the eggs to fall off, rinsing the rinsing liquid for 1-4 times, washing the eggs with sterilized distilled water, collecting the eggs on moisture-preserving filter paper, and then placing the moisture-preserving filter paper in a self-sealing bag to wait for larvae to hatch out;
(7) and (4) after the larvae are hatched, repeating the steps 1, 2, 3, 4, 5 and 6 in sequence, and circulating to finish subculture.
6. The feeding method according to claim 5, wherein: the diameter of the bottom of each hole of the feed tray is 1-3 cm, and the depth of each hole is 3-5 cm; the container in the step (4) is a plastic bottle with the diameter of 3-5 cm and the height of 3-8 cm, and a hole is formed in the upper surface of the plastic bottle; the rinsing liquid used for rinsing is an aqueous solution containing 0.12-0.16 g/L of sodium hypochlorite and 6.0-7.2 g/L of sodium chloride.
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