CN108849763A - A kind of peach horn worm method for breeding - Google Patents

A kind of peach horn worm method for breeding Download PDF

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Publication number
CN108849763A
CN108849763A CN201810792589.1A CN201810792589A CN108849763A CN 108849763 A CN108849763 A CN 108849763A CN 201810792589 A CN201810792589 A CN 201810792589A CN 108849763 A CN108849763 A CN 108849763A
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CN
China
Prior art keywords
peach
breeding
ovum
leaf
hawkmoth
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CN201810792589.1A
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Chinese (zh)
Inventor
张华普
张怡
李占文
姜彩鸽
樊仲庆
王国珍
宋双
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Institute of Plant Protection of Ningxia Academy of Agriculture and Forestry Sicience
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Institute of Plant Protection of Ningxia Academy of Agriculture and Forestry Sicience
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Priority to CN201810792589.1A priority Critical patent/CN108849763A/en
Publication of CN108849763A publication Critical patent/CN108849763A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention belongs to agricultural insect breeding technology fields, disclose a kind of peach horn worm method for breeding.Include the following steps:(1) worm's ovum is collected;(2) the ovum phase raises:First oviposition is placed in 33 DEG C of weather incubator and is hatched, newly hatched larvae is obtained;(3) larval phase, is raised:It will be raised in 29 DEG C of weather incubators of newly hatched larvae dislocation, until raising until 3 ages;It will be raised in 25 DEG C of 3 instar larvae dislocation of weather incubator, until raising until 6 instar larvaes.Humidity is 55% in above-mentioned breeding process mesoclimate incubator, and the photoperiod is 14L:10D, intensity of illumination are 1500lx.Compared with prior art, the beneficial effect of the application is that method for breeding is simple, and the survival rate of larva is high, especially can be improved the survival rate of low instar larvae, and then can provide a large amount of host for peach hawkmoth disk braconid wasp parasitism.

Description

A kind of peach horn worm method for breeding
Technical field
The invention belongs to agricultural insect breeding technology field, in particular to a kind of peach horn worm method for breeding.
Background technique
Peach hawkmoth is also known as peach horn worm, jujube hawkmoth, peach sparrow etc., belongs to Lepidoptera, Sphingidae, mainly with larva gnaw jujube, The fruit tree leafs such as peach, apple, pears, apricot, wild jujube, grape, pears and cherry are harm, belong to polyphagous pest-insect.Larva 1 year 2 generations of generation, Serious with the harm of 2nd generation larva, time of origin is July~August.Larva body is big, eats a lot, totally 6 age, and the 4th age started as gluttony Phase often eats up blade by branch, and is shifted after treetop spray is gnawed light, eats up full leaf piece when seriously occurring, Tree body photosynthetic area and nutrient accumulation is caused to reduce, yield decline, tree vigo(u)r decline.Investigation shows that peach hawkmoth is equal in the Ningxia whole district It is distributed.7~8 days its ovum phases, every female moth egg 150~450 have stronger fertility.
At present both at home and abroad in the improvement of peach hawkmoth, chemistry of pesticide means and insecticidal lamp are relied primarily on.Pesticide is killing peach While hawkmoth, but also field natural enemy quantity falls sharply, and with the long-time service of pesticide, peach hawkmoth itself can also generate anti-medicine Property, to great outburst occur, form more serious cause harm.Meanwhile pesticide can also form the environment such as water, soil and destroy;Residual Pesticide in fruit etc. also will form pesticide residue.Insecticidal lamp only has trapping effect to peach hawkmoth adult, to larva without any Effect, and caused by peach hawkmoth is mainly larva the harm of trees, so the effect of insecticidal lamp and its limited;In addition, desinsection Lamp price, trapping are limited in scope, are easy to be lost.Therefore, exploitation biological control method prevention and control peach hawkmoth harm, environmental protection and There is major application value in food safety.
Natural enemy insect is the important component of biological control, plays particularly important tune in control pest population density Control effect.Find that peach horn worm has a kind of natural parasite --- peach hawkmoth disk braconid wasp, endoparasitism, development in research Period is short.Field investigation shows that peach hawkmoth disk braconid wasp can reach 30% or so, Er Qieduo to peach horn worm mean parasitized rate Before number peach hawkmoth dies of serious harm by parasitic larva, this has weight to its harm of the population quantity and inhibition of control peach hawkmoth It acts on, with good development and application prospects.
Natural enemy large-scaled propugation is the important link in natural enemy development and utilization work, is the premise for carrying out Field information release; And to carry out natural enemy scale and expand numerous required a large amount of natural enemy host, therefore, the present invention provides a kind of peach horn worm method for breeding, Host source is provided for large-scale breeding peach hawkmoth disk braconid wasp, to reach development and application peach hawkmoth disk braconid wasp to prevent and treat peach The purpose of hawkmoth.
Summary of the invention
It is an object of the present invention to for a large amount of peach hawkmoth hosts needed for existing large-scale breeding peach hawkmoth disk braconid wasp and A kind of peach horn worm method for breeding developed.
To achieve the goals above, the technical solution that the application uses for:A kind of peach horn worm method for breeding, including such as Lower step:
S1, worm's ovum is collected
Peach hawkmoth adult is traped in late May~late July, and collects the ovum of peach hawkmoth adult;
S2, the raising of ovum phase
Select the same period first, well-developed just oviposition is placed in culture dish, then, covered in first oviposition fresh Blade, finally will just oviposition be placed in 33 DEG C of temperature, humidity 55%, photoperiod 14L:The weather of 10D, intensity of illumination 1500lx Hatch in incubator, ovum is known as newly hatched larvae once hatching, and immediately separately raises newly hatched larvae with ovum;
S3, larval phase raising
Immediately by 29 DEG C of newly hatched larvae dislocation, humidity 55%, photoperiod 14L:The weather training of 10D, intensity of illumination 1500lx It supports and is raised in case, until raising until 3 ages;
By 25 DEG C of 3 instar larvae dislocation, humidity 55%, photoperiod 14L:10D, intensity of illumination 1500lx weather incubator in Raising, until raising until 6 instar larvaes.
Further, the newly hatched larvae is placed on Φ 9mm culture dish and is raised, and 3~6 instar larvaes are placed on Φ 15mm Culture dish is raised.
Further, the blade is jujube leaf, peach leaves, apple leaf, leaf of pear tree, Folium Pruni, spina date leaf, vine One of leaf and cherry leaf.
Further, peach hawkmoth adult is trapped using frequency ventilating type solar insect-killing light in the S1.
Further, the weather incubator is intelligent weather incubator.
Compared with prior art, the beneficial effects of the invention are as follows:The peach horn worm method for breeding of the application is easy to operate, The survival rate of larva is high, and especially the survival rate of low instar larvae is high, can provide for peach hawkmoth disk braconid wasp parasitism and largely post It is main.
Specific embodiment
Technological means of the invention, creation characteristic, achieving the goal is easy to understand with effect in order to make, below in conjunction with Technical solution of the present invention is clearly and completely described in the specific embodiment of the invention.
Embodiment
A kind of peach horn worm method for breeding, includes the following steps:
S1, worm's ovum is collected
Frequency ventilating type solar insect-killing light (PS-15VI-2 type) is utilized in late May~late July, traps peach hawkmoth adult Oviposition, adult amount is more to trap mid-June~mid-July, and usual peach hawkmoth produces ovum in the insect collecting bag of insecticidal lamp Side pocket wall on, and ovum is in middle part uniformly dispersing, easily collecting.After collecting peach hawkmoth ovum grain, takes back interior and be put into intelligent weather training It supports and is saved in case.The ovum of different time is separated, carries out label, when the time does not know, can sentence according to the external appearance characteristic of ovum grain Disconnected egg-laying time.
S2, the raising of ovum phase
Firstly, from the ovum of collection, the same period is selected, well-developed just oviposition is placed in culture dish, then, first Fresh blade is covered in oviposition, and first oviposition is finally placed in 33 DEG C, humidity 55%, photoperiod 14L:10D, intensity of illumination Hatching in the intelligent weather incubator (MLR-352H-PC type) of 1500lx, ovum are known as newly hatched larvae once hatching, and immediately will be first It incubates larva and is separately raised with ovum;
It is required to the newly hatched larvae that point removal has just been hatched daily, and replaces fresh blade for ovum;
Each newly hatched larvae, which is placed in an independent Φ 9mm culture dish, to be continued to raise, and is fed with fresh blade Newly hatched larvae, it is ensured that newly hatched larvae has food can be with feeding, and larva can be avoided to climb by larva " locking " in culture dish Culture dish out.
S3, larval phase raising
Because the death rate of low instar larvae is higher, to strengthen management.It, immediately will be first after newly hatched larvae just hatches It incubates larva and is separately positioned at the culture dish dislocation that an independent Φ 9mm culture dish continues to raise, while will fill newly hatched larvae 29 DEG C, humidity 55%, photoperiod 14L:The middle raising of weather incubator (MLR-352H-PC type) of 10D, intensity of illumination 1500lx, Until raising until 3 ages;
After larva entered for 3 age, larva is transferred in Φ 15mm culture dish and continues to raise, each culture dish raising one A larva;And the culture dish for filling 3 instar larvaes is placed on 25 DEG C, photoperiod 14L:The intelligent gas of 10D, intensity of illumination 1500lx Wait raising in incubator (MLR-352H-PC type);It needs to provide sufficient fresh blade during raising, replaces one daily Secondary blade, and excrement is cleared up, until raising until 6 instar larvaes.
It should be noted that blade used in raising is with petiolate blade, petiole is wrapped up with wet cotton balls, blade surface There is no water, if there is water on blade surface, dead larva can be glued.Because of low instar larvae fragility, 1~2 instar larvae need not when replacing blade Single head larva is shifted, fresh blade is directly covered on original blade, peach horn worm, which is understood, oneself climbs to fresh leaf On piece.When having to transfer larva, polypide is gently stirred by writing brush or insect tweezer, moves to fresh leaf on piece.
As a preferred embodiment of the invention, blade is jujube leaf, peach leaves, apple leaf, leaf of pear tree, Folium Pruni, wild jujube One of leaf, grape leaf and cherry leaf.
In order to probe into influence of the different temperatures to the hatching rate of ovum and the survival rate of larva, it is real that we have done following comparison It tests.
Comparative example 1
Comparative example 1 and the difference of embodiment are:When comparative example 1 raises peach hawkmoth, the raising of ovum phase and larva are raised During supporting, carry out at that same temperature;And embodiment is to the temperature during the raising of peach hawkmoth ovum phase and larva raising It is different.Remaining method for breeding of comparative example 1 is identical as embodiment.
Comparative example 1 is provided with 4 for trying temperature, respectively 20 DEG C, 25 DEG C, 28 DEG C and 33 DEG C altogether.We supply this four The survival rate of egg hatching rate and each instar larvae counts at a temperature of examination, statistical result such as table 1.
Peach hawkmoth egg hatching rate and its larva survival rate under the different constant temperature of table 1
As known from Table 1, (1) peach hawkmoth can complete to develop at a temperature of 4 for trying, but ovum incubates under different temperatures Rate is different with the survival rate of each instar larvae.(2) hatching rate of ovum highest at 33 DEG C;The survival rate of low age (1~2 age) larva The highest at 28 DEG C;The survival rate of 3~6 instar larvaes highest at 25 DEG C.The survival rate of low age (1~2 age) larva at (3) 33 DEG C It is relatively low;The hatching rate of ovum is relatively low at 28 DEG C;At 25 DEG C the survival rate of the hatching rate of ovum and low instar larvae relatively compared with It is low;The hatching rate of ovum and the survival rate of low instar larvae are extremely low at 20 DEG C.
In order to further probe into influence of the temperature to low age (1~2 age) larva survival rate, we have been done as follows to having a competition It tests:
Comparative example 2
The ovum phase raising of comparative example 2 is identical as embodiment, i.e., is to raise at 33 DEG C to ovum.Comparative example 2 and reality The difference for applying example is:Comparative example 2 at a constant temperature raises larva, and embodiment is when to larva raising It is the temperature raising different according to the different set in larva worm age.
Comparative example 2 is provided with 3 for trying temperature, respectively 28 DEG C, 29 DEG C and 30 DEG C altogether.We are to this 3 for trying temperature The survival rate of lower low instar larvae, 3 instar larvaes and 4 instar larvaes counts, statistical result such as table 2.
The survival rate of peach hawkmoth low instar larvae under 2 different temperatures of table
As known from Table 2, the survival rate of low instar larvae highest at 29 DEG C.
Intensity of illumination is also visited to during peach horn worm raising in the peach horn worm method for breeding of the application Study carefully, therefore do following comparative experiments, probes into influence of the intensity of illumination to peach horn worm survival rate.
Comparative example 3
Comparative example 3 is only that intensity of illumination is different from the difference of embodiment.Comparative example 3 is provided with 3 strong for examination illumination altogether Degree, respectively 1500lx, 3000lx and 14000lx.We unite to these three survival rates for larva under examination intensity of illumination Meter, as a result such as table 3.
The survival rate of peach horn worm under the different light intensity of table 3
By table 3 it is found that peach horn worm only intensity of illumination be 1500lx when, the survival rate highest of larva.
In conclusion by the way that 1~comparative example of comparative example 3 is compared, it has been found that only use feeding described herein The method of supporting (by ovum, low instar larvae and 3~6 instar larvaes be placed in it is different at a temperature of raise, while intensity of illumination is 1500lx), the survival rate of the hatching rate and peach horn worm that just can ensure that peach hawkmoth ovum reaches maximization, can especially ensure The survival rate of low instar larvae reaches maximization, so as to provide a large amount of host for peach hawkmoth disk braconid wasp parasitism;And this The peach horn worm method for breeding of application is easy to operate, and rearing conditions are easy to control.
Disclosed above is only presently preferred embodiments of the present invention, and still, the embodiment of the present invention is not limited to this, Ren Heben What the technical staff in field can think variation should all fall into protection scope of the present invention.

Claims (5)

1. a kind of peach horn worm method for breeding, which is characterized in that include the following steps:
S1, worm's ovum is collected
Peach hawkmoth adult is traped in late May~late July, and collects the ovum of peach hawkmoth adult;
S2, the raising of ovum phase
Select the same period first, well-developed just oviposition is placed in culture dish, then, cover fresh leaf in first oviposition First oviposition is finally placed in 33 DEG C of temperature, humidity 55%, photoperiod 14L by piece:The weather incubator of 10D, intensity of illumination 1500lx Middle hatching, ovum is known as newly hatched larvae once hatching, and immediately separately raises newly hatched larvae with ovum;
S3, larval phase raising
By 29 DEG C of newly hatched larvae dislocation, humidity 55%, photoperiod 14L:10D, intensity of illumination 1500lx weather incubator in raise It supports, until raising until 3 ages;
By 25 DEG C of 3 instar larvae dislocation, humidity 55%, photoperiod 14L:10D, intensity of illumination 1500lx weather incubator in raise It supports, until raising until 6 instar larvaes.
2. a kind of peach horn worm method for breeding as described in claim 1, which is characterized in that the newly hatched larvae is placed on Φ 9mm culture dish is raised, and 3~6 instar larvaes are placed on Φ 15mm culture dish and are raised.
3. a kind of peach horn worm method for breeding as described in claim 1, which is characterized in that the blade is jujube leaf, peach One of leaf, apple leaf, leaf of pear tree, Folium Pruni, spina date leaf, grape leaf and cherry leaf.
4. a kind of peach horn worm method for breeding as described in claim 1, which is characterized in that in the S1 too using frequency ventilating type Positive energy insecticidal lamp traps peach hawkmoth adult.
5. a kind of peach horn worm method for breeding as described in claim 1, which is characterized in that the weather incubator is intelligence Weather incubator.
CN201810792589.1A 2018-07-18 2018-07-18 A kind of peach horn worm method for breeding Pending CN108849763A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3338704B1 (en) * 2002-01-31 2002-10-28 株式会社九州メディカル Method of treating plant food waste using larvae of Lepidoptera noctuids, and feed using the product
CN104186430A (en) * 2014-08-14 2014-12-10 中国热带农业科学院椰子研究所 Method for feeding a large number of laboratory test populations of Opisina arenosella Walkers
CN106857414A (en) * 2017-04-06 2017-06-20 海南省农业科学院植物保护研究所 The mass rearing method of prodenia litura
CN108125074A (en) * 2018-03-14 2018-06-08 河南省农业科学院植物保护研究所 Double committee's noctuid man-made feeds and its method for breeding

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3338704B1 (en) * 2002-01-31 2002-10-28 株式会社九州メディカル Method of treating plant food waste using larvae of Lepidoptera noctuids, and feed using the product
CN104186430A (en) * 2014-08-14 2014-12-10 中国热带农业科学院椰子研究所 Method for feeding a large number of laboratory test populations of Opisina arenosella Walkers
CN106857414A (en) * 2017-04-06 2017-06-20 海南省农业科学院植物保护研究所 The mass rearing method of prodenia litura
CN108125074A (en) * 2018-03-14 2018-06-08 河南省农业科学院植物保护研究所 Double committee's noctuid man-made feeds and its method for breeding

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Title
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鞠瑞亭 等: "温度对褐边绿刺蛾实验种群生长发育及存活的影响", 《植物保护学报》 *

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Application publication date: 20181123