CN104186430A - Method for feeding a large number of laboratory test populations of Opisina arenosella Walkers - Google Patents
Method for feeding a large number of laboratory test populations of Opisina arenosella Walkers Download PDFInfo
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- CN104186430A CN104186430A CN201410397993.0A CN201410397993A CN104186430A CN 104186430 A CN104186430 A CN 104186430A CN 201410397993 A CN201410397993 A CN 201410397993A CN 104186430 A CN104186430 A CN 104186430A
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Abstract
The invention belongs to the technical field of insect feeding and particularly discloses a method for feeding a large number of laboratory test populations of Opisina arenosella Walkers. The method is an improvement on an existing method for feeding the Opisina arenosella Walkers according to mating and oviposition characteristics of the Opisina arenosella Walkers. Mating space is enlarged, and the number of matched pairs of male and female Opisina arenosella Walkers is increased, so that female Opisina arenosella Walkers are stimulated to oviposit. In addition, the number of places where the female Opisina arenosella Walkers can oviposit is increased, the oviposition area is enlarged, and the survival rate of ova is increased. Tedious procedures of collecting ova through independent test tubes and leaf replacement during larva feeding are simplified, workload is decreased greatly, and the large number of standard test populations of Opisina arenosella Walkers with a high survival rate and simplified procedures can be obtained truly.
Description
Technical field
The present invention relates to technical field of insect feeding, more specifically, a kind of method that relates to coconut and knit the laboratory test population mass rearing of moth.
Background technology
Coconut knit moth (
opisina arenosellawallker) be that Exotic pests is newly invaded by China, belong to Lepidoptera (Lepidoptera), Zhi E section (Oecophoridae), coconut is knitted the English coconut blackheaded caterpillar by name of moth, and Chinese translation has coconut blackhead crawler belt worm, food leaf crawler belt worm and coconut palm moth moth.It is the important pests on palm plant that coconut is knitted moth, within 2014, is listed in exotic invasive pest list by the State Administration of Forestry.This worm original producton location is in India, Sri Lanka and Burma, serious harm coconut palm.Coconut was knitted moth and had been spread the Thailand and the Malaysia that spread to ASEAN countries in recent years, and especially in Thailand, this worm harm is serious.This insect takes food palm plant Lao Ye and young leaves, causes plant leaf withered, occurs fallen leaves, when serious, can cause whole strain death.In August, 2013, coconut is knitted moth and is found first in China Behavioral Change, and be diffused into Haikou, the cities and counties such as Danzhou, Bao Ting, Sanya, Qionghai, Wenchang, and Guangdong and Guangxi province.
The method of knitting moth mass rearing about coconut in prior art is not a lot.Research Institute of Environment and Plant Protection, Chinese Academy of Tropi discloses a kind of coconut and has knitted moth mass rearing method, its breeding technology is as follows: (1) coconut is knitted the raising of moth adult and brought out it and lays eggs: the segment that 1. cocoanut tree Lao Ye is cut into 8cm, put into glass tube (long 8.5cm, diameter 4.5cm), 2 of every pipes; 2. in each glass tube, introduce and a pair ofly just incubate coconut and knit moth adult, with the cotton swab that is moistened with 10% hydromel be that coconut is knitted moth Adult supplement nutrient, glass tube seals with 200 order gauzes; 3. after 1-2 days, coconut is knitted moth and is started to lay eggs, and ovum is scheduling in blade surface, often femalely on average lays eggs 200, when change blade every day, the blade with ovum is chosen, and puts into another insect box with a collection of concentrating, and every box is put 1000 ovum; (2) raising of ovum hatching and 1 instar larvae: 1. ovum started hatching after 6-7 days is sneaked into the blade with ovum by the long cocoanut tree Lao Ye of 10 15cm before hatching, and newly hatched larvae can automatically be climbed on new replacing blade and take food, and within every 3 days, changes primary vane; (3) larval phase, raises and the management of pupa: 1. 1-3 instar larvae, every box is raised 1000, within every 4 days, changes 1 blade, with the Lao Ye of young leaves mixed zone worm, by the Lao Ye with worm not with choose with the dead worm of mildew; 2. 4-8 instar larvae, every box is raised 200, within every 4 days, changes 1 blade, with the Lao Ye of young leaves mixed zone worm, by the Lao Ye with worm not with choose with the dead worm of mildew; 3. 50 days larval phases, larvae pupation after 50 days, be 10 days pupa time, do not need to change blade, but will keep ambient humidity; Turn into after adult at pupa, match and put into glass tube, add blade and honey to induce it to lay eggs, enter next captive breeding from generation to generation.
Also there are some defects in above-mentioned technology, male imago mating as female in (1) space is too small: in prior art, coconut is knitted to every pair of the female male worm of moth and put into separately long 8.5cm, in the glass tube of diameter 4.5cm, raise, and find according to our research: the adult mating that coconut is knitted moth needs certain space, before mating, need male worm to carry out nuptial flight " ceremony ", female worm assortative mating male worm according to this, will contribute to stimulate female worm mate and oviposit through this ceremony; Also finding in addition that coconut is knitted moth can polyandry, and then improves egg laying amount, and 1 female 1 male pairing in prior art, has had a strong impact on the egg laying amount of female male worm mating probability and female worm.(2) place that can lay eggs for female worm is few, area is little: in prior art, the coconut blade of putting into 2 8cm in the glass tube of female male worm mating is laid eggs for female worm, because of test tube space little, blade area is limited, have a strong impact on the female worm quantity of laying eggs, and on blade, ovum is seriously stacked, greatly reduces egg hatching rate.(3) workload is large, process complexity, overall feeding efficiency is low: raise because prior art adopts female male worm single tube, be required to be each glass tube every day and change the blade of laying eggs, if need mass rearing, collecting the pairing of adult and every day, change the blade of laying eggs will increase very large workload.
Summary of the invention
The present invention is in order to overcome the above-mentioned deficiency of prior art, and the method for the laboratory test population mass rearing that a kind of coconut knits moth is provided.
As the insect of new invasion, to its biology, ecological habit, the research that the rule of harm occurs and prevent and treat method all needs a large amount of worms source, and therefore the present invention can realize the mass rearing in standardization test worm source, and in order to carry out, coconut is knitted moth correlative study and the method for preventing and treating provides basis.
To achieve these goals, the present invention is achieved by following scheme.
Coconut is knitted a method for the laboratory test population mass rearing of moth, comprises the steps:
S1. the raising of adult and laying eggs:
S11. at high 30cm, in the cylindrical transparent containers of diameter 25cm, put into 5 pairs of male female worms of the not mating of newly sprouting wings, in each container, put into the long healthy cocoanut tree blade of 10 15cm, be that coconut is knitted moth Adult supplement nutrient with the cotton balls that is moistened with 10% hydromel, vessel port is sealed with gauze simultaneously;
S12. within every 3 days, change 1 blade, change cotton balls every day 1 time, after 1 ~ 2 day, coconut is knitted moth and is started to lay eggs, and the blade with ovum and gauze is chosen in every 3 days, and with a collection of larva insect box of putting into long 30cm, wide 20cm of concentrating, every box is put 1000 ovum;
S2. ovum started hatching after 6-7 days, and now adding length is 20, the blade of 15cm, waited for that newly hatched larvae is climbed on new replacing blade automatically to take food, and within every 3 days, changed primary vane;
S3. the raising of Larva and pupa
S31. by 1-3 instar larvae, continue to raise in the insect box of long 30cm, wide 20cm, 1000, every box, changes 1 blade for every 7 days, with the Lao Ye of young leaves mixed zone worm, by the Lao Ye with worm not with choose with the dead worm of mildew;
S32. by 4-8 instar larvae, change in long 40cm, wide 30cm insect box, 400, every box, changes 1 blade for every 4 days, with the Lao Ye of young leaves mixed zone worm, by the Lao Ye with worm not with choose with the dead worm of mildew;
S32. 50 days larval phases, larvae pupation after 50 days, be 10 days pupa time, do not need to change blade, but will keep ambient humidity; In the time that pupa turns into adult soon, match and put into cylindrical transparent containers, add new blade and honey cotton balls, allow it lay eggs, enter next captive breeding from generation to generation.
The cylindrical transparent containers of the high 30cm of the present invention, diameter 25cm provides the nuptial flight space of male worm, and can meet 1 female many male mating patterns, has greatly improved the success rate of male and female mating, the female worm quantity of laying eggs; In addition, each container inner wall surrounding is fixed the long coconut blade of 10 15cm and is laid eggs for female worm, and container top adopts double-deck 200 order gauze sealings.According to our research, female worm is except coconut blade is laid eggs, and in gauze hole, can lay eggs in a large number in seam place.This technology has increased female worm spawning place area greatly, has improved female worm egg laying amount, and because the space of laying eggs is large, area is wide, ovum does not need excessively stacked, and survival rate of ovum is improved greatly.
Within every 3 days, collect the blade and have the sealing of a laying eggs gauze of laying eggs in each container, jointly put into insect box (the long 40cm that enough fresh coconut blades are housed, wide 30cm), change weekly primary vane, choose to have taken food and be not with the cured leaf sheet of worm and mouldy bad blade to choose.This technology has been simplified the complicated procedures of independent test tube receipts ovum and larva raising replacing blade, has greatly reduced workload, has really realized coconut and has knitted the mass rearing of moth population.
It is the newfound Alien Invasive Species of China in 2014 that coconut is knitted moth, and its indoor mass rearing technology is still immature.In existing breeding technology: every pair of (1) female male imago is put into separately in glass tube (long 8.5cm, diameter 4.5cm) and raised; (2) the coconut blade of in vitro putting into 2 8cm is laid eggs for female worm; (3) needing to collect the blade of laying eggs of each test tube puts into insect box again and carries out larva raising.
But find according to our research: the adult mating that coconut is knitted moth needs certain space, needs male worm to carry out nuptial flight " ceremony " before mating, female worm assortative mating male worm according to this, will contribute to female worm to lay eggs through this ceremony; Also find that in addition coconut knits moth and can lay eggs by polyandry.In prior art, 1 female 1 hero is put into small space mate and oviposit, has affected female male worm Mating success.In prior art, each long coconut blade of 2 8cm of in vitro only putting into is laid eggs for female worm in addition, and little because of test tube space, blade area is limited, has a strong impact on the female worm quantity of laying eggs, and likely makes ovum stacked, increases egg mortality.The blade of laying eggs of changing each glass tube every day, for mass rearing, workload is too large, and program is too complicated.
Compared with prior art, the present invention has following beneficial effect:
The present invention knits moth mating habit according to coconut, increases mating space, increases female male worm pairing number, stimulates female worm to lay eggs.Adult mate and oviposit place adopts cylinder transparent type container (high 30cm, diameter 25cm), puts into 5 pairs of female worms of hero in every container.This technology provides the nuptial flight space of male worm, and can meet 1 female many male mating patterns, has greatly improved the success rate of male and female mating, the female worm quantity of laying eggs;
The present invention increases female worm spawning place, increases the area of laying eggs, and improves survival rate of ovum.Each container inner wall surrounding is fixed the long coconut blade of 10 15cm and is laid eggs for female worm, and container top adopts double-deck 200 order gauze sealings.According to our research, female worm is except coconut blade is laid eggs, and in gauze hole, can lay eggs in a large number in seam place.This technology has increased female worm spawning place area greatly, has improved female worm egg laying amount, and because the space of laying eggs is large, area is wide, ovum does not need excessively stacked, and survival rate of ovum is improved greatly.
Simplify and raise flow process, reduce workload, really realize conveniently mass rearing.Within every 3 days, collect the blade and have the sealing of a laying eggs gauze of laying eggs in each container, within every 4-7 days, change an insect box intra vane, choose to have taken food and be not with the cured leaf sheet of worm and mouldy bad blade to choose.This technology has been simplified independent test tube and receives ovum and larva raising and change the complicated procedures of blade, has greatly reduced workload, can really realize and obtain that survival rate is high, program simplification, a large amount of standardized coconut knit moth Experimental population.
embodiment
Further illustrate the present invention below in conjunction with specific embodiment, but embodiment does not limit in any form to the present invention.Unless stated otherwise, the method and apparatus that the present invention adopts is the art conventional method and equipment.Unless stated otherwise, agents useful for same of the present invention and material are commercial.
embodiment 1
S1. coconut is knitted the raising of moth adult and is laid eggs:
S11. in cylindrical transparent containers (high 30cm, diameter 25cm), put into male worm and the female worm of 5 pairs of not mating of newly sprouting wings.
S12. healthy cocoanut tree blade is cut into 15cm and grow, put 10 in each container, the cotton balls that use is moistened with 10% hydromel is that coconut is knitted moth Adult supplement nutrient, and vessel port is sealed with 200 order gauzes.
S13. within every 3 days, change 1 blade, change the cotton balls that is moistened with 10% hydromel for 1 time every day, honey extends coconut and knits the moth life-span, and improves egg laying amount and survival rate;
S14. after 1-2 days, coconut is knitted moth and is started to lay eggs, and ovum is scheduling on the sealing gauze of blade surface and container, the blade with ovum and gauze is chosen in every 3 days, puts into long 30cm with a collection of concentrating, and in the larva insect box of wide 20cm, every box is put 1000 ovum;
S2. the raising of ovum hatching and 1 instar larvae
The ovum of step S1 results started hatching after 6-7 days, and now adding length is 20, the blade of 15cm, waited for that newly hatched larvae is climbed on new replacing blade automatically to take food, and within every 3 days, changed primary vane;
S3. the raising of Larva and pupa
S31. by 1-3 instar larvae, continue at long 30cm, in the insect box of wide 20cm, raise, 1000, every box, changes 1 blade for every 7 days, with the Lao Ye of young leaves mixed zone worm, by the Lao Ye with worm not with choose with the dead worm of mildew;
S32. by 4-8 instar larvae, change to long 40cm, in wide 30cm insect box, 400, every box, changes 1 blade for every 4 days, with the Lao Ye of young leaves mixed zone worm, by the Lao Ye with worm not with choose with the dead worm of mildew;
S33. 50 days larval phases, larvae pupation after 50 days, be 10 days pupa time, do not need to change blade, but will keep ambient humidity; In the time that pupa turns into adult soon, match and put into cylindrical transparent containers, add new blade and honey cotton balls, allow it lay eggs, enter next captive breeding from generation to generation.
Knit the healthy development of moth in order to meet coconut, each worm state raising condition is 25～27 DEG C of temperature, relative moisture 70% ± 10%, natural lighting.
The insect box (30cm × 20cm × 8cm, 40 × 30cm × 10cm) of two kinds of sizes of the present invention, being provided with long in lid central authorities is 15cm, the wide rectangle hole for 8cm, and the upper 200 object gauzes that fasten with glue.Described foster worm frame is made up of universal steel angle, long 2 m, wide 0.6 m, high 1.8 m.Each brandreth divides four layers, the bottom is liftoff 0.2 m, and every interlamellar spacing 0.4 m, is furnished with 1 40w fluorescent tube for every layer; Four legs stand on the pallet being filled with water, and prevent that the coconut of ant infringement raising from knitting moth.
In order to prevent that coconut from knitting in moth feeding process, the generation of popular disease, 3% hydrogen peroxide spraying disinfection is monthly used in insectary; Breeding facility is also regularly used 70% alcohol disinfecting.
Coconut described in embodiment 1 is knitted the raising process result of the test of moth amount reproduction method as shown in table 1.
Table 1 coconut is knitted the each worm state of moth feeding effect
The result drawing according to table 1, can reach a conclusion: coconut is knitted the incubation rate average out to 84.50% of moth ovum, the survival rate average out to 98.67% of 1 instar larvae, the survival rate average out to 95.24% of 2 instar larvaes, survival rate average out to 90.71%, 4 instar larvae survival rate average out to 89.57%, the 5 instar larvae survival rate average out to 83.07% of 3 instar larvaes, 6 ~ 8 instar larvae survival rate average out to 72.14%, the survival rate average out to 88.85% of pupa.
Claims (3)
1. coconut is knitted a method for the laboratory test population mass rearing of moth, it is characterized in that, comprises the steps:
S1. the raising of adult and laying eggs:
S11. at high 30cm, in the cylindrical transparent containers of diameter 25cm, put into 5 pairs of male female worms of the not mating of newly sprouting wings, in each container, put into the long healthy cocoanut tree blade of 10 15cm, be that coconut is knitted moth Adult supplement nutrient with the cotton balls that is moistened with 10% hydromel, vessel port is sealed with gauze simultaneously;
S12. within every 3 days, change 1 blade, change cotton balls every day 1 time, after 1 ~ 2 day, coconut is knitted moth and is started to lay eggs, and the blade with ovum and gauze is chosen in every 3 days, and with a collection of larva insect box of putting into long 30cm, wide 20cm of concentrating, every box is put 1000 ovum;
S2. ovum started hatching after 6-7 days, and now adding length is 20, the blade of 15cm, waited for that newly hatched larvae is climbed on new replacing blade automatically to take food, and within every 3 days, changed primary vane;
S3. the raising of Larva and pupa
S31. by 1-3 instar larvae, continue to raise in the insect box of long 30cm, wide 20cm, 1000, every box, changes 1 blade for every 7 days, with the Lao Ye of young leaves mixed zone worm, by the Lao Ye with worm not with choose with the dead worm of mildew;
S32. by 4-8 instar larvae, change in long 40cm, wide 30cm insect box, 400, every box, changes 1 blade for every 4 days, with the Lao Ye of young leaves mixed zone worm, by the Lao Ye with worm not with choose with the dead worm of mildew;
S32. 50 days larval phases, larvae pupation after 50 days, be 10 days pupa time, do not need to change blade, but will keep ambient humidity; In the time that pupa turns into adult soon, match and put into cylindrical transparent containers, add new blade and honey cotton balls, allow it lay eggs, enter next captive breeding from generation to generation.
2. coconut according to claim 1 is knitted the method for the laboratory test population mass rearing of moth, it is characterized in that, and high 30cm, the cylindrical transparent containers of diameter 25cm provides nuptial flight space for male worm, and can meet 1 female many male mating patterns.
3. coconut according to claim 1 is knitted the method for the laboratory test population mass rearing of moth, it is characterized in that, and double-deck 200 order gauze sealings for cylindrical transparent containers, female worm is except coconut blade is laid eggs, and in gauze hole, can lay eggs in a large number in seam place.
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| CN105145495A (en) * | 2015-09-10 | 2015-12-16 | 延安大学 | Artificial breeding method for jujube tree porthesia similis |
| CN107279073A (en) * | 2017-08-17 | 2017-10-24 | 广州市林业和园林科学研究院 | A kind of coconut knits the indoor feeding method of moth |
| CN108770790A (en) * | 2018-03-23 | 2018-11-09 | 西南林业大学 | A kind of Dendrolimus kikuchii method for breeding |
| CN108849763A (en) * | 2018-07-18 | 2018-11-23 | 宁夏农林科学院植物保护研究所（宁夏植物病虫害防治重点实验室） | A kind of peach horn worm method for breeding |
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| CN103843724B (en) * | 2014-01-26 | 2015-09-23 | 西北农林科技大学 | A kind of artificial group breeding method of prodenia litura |
| CN103960201A (en) * | 2014-05-12 | 2014-08-06 | 山西省农业科学院农产品质量安全与检测研究所 | Plutella xylostella indoor reproduction method |
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| CN105145495A (en) * | 2015-09-10 | 2015-12-16 | 延安大学 | Artificial breeding method for jujube tree porthesia similis |
| CN105145495B (en) * | 2015-09-10 | 2017-10-27 | 延安大学 | Jujube tree steals the artificial breeding method of poison moth |
| CN107279073A (en) * | 2017-08-17 | 2017-10-24 | 广州市林业和园林科学研究院 | A kind of coconut knits the indoor feeding method of moth |
| CN107279073B (en) * | 2017-08-17 | 2021-04-23 | 广州市林业和园林科学研究院 | Indoor breeding method for coconut leaf moths |
| CN108770790A (en) * | 2018-03-23 | 2018-11-09 | 西南林业大学 | A kind of Dendrolimus kikuchii method for breeding |
| CN108849763A (en) * | 2018-07-18 | 2018-11-23 | 宁夏农林科学院植物保护研究所（宁夏植物病虫害防治重点实验室） | A kind of peach horn worm method for breeding |
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