CN103766288B - The a large amount of propagation method of moth knitted by coconut - Google Patents

Abstract

The invention relates to a large-scale breeding method of the coconut weaver moth. The breeding of each insect state of the coconut weaver moth is carried out under the conditions of a temperature of 25-27°C, a relative humidity of 75%±10%, and natural light. The newly hatched coconut moths are paired with males and females, introduced into the glass tube with old coconut leaves, and 10% honey water is provided to supply nutrients; after 1-2 days, the coconut moths lay eggs on the coconut leaves; the leaves with eggs are picked out , put them into an insect breeding box, with 1,000 eggs per box; the eggs can hatch into larvae after 6-7 days, and the leaves are replaced every 4 days; they pupate after 50 days, and pupae emerge into adults after 9-12 days, and enter the next A cyclic breeding process. The invention uses the old leaves of the coconut tree to raise the larvae, and the operation is simple and practical, and the instars of the weaver moth can be reproduced in batches in a continuous, balanced and batch manner, providing a large number of insect sources for the basic research and prevention and control of the weaver moth.

Description

Mass reproduction method of coconut weaver moth

technical field

The invention relates to a feeding and breeding method of insects, in particular to a mass breeding method of the weaver moth.

Background technique

Coconut weaving moth (Opisina arenosella Wallker) is a new invasive alien pest in my country. It belongs to Lepidoptera (Lepidoptera), Oecophoridae (Oecophoridae). Coconut moth. Coconut weaver moth is an important pest on palm plants. In India, Sri Lanka and Myanmar, the coconut weaver moth is a serious threat to coconut trees. In recent years, the coconut weaver moth has spread to Thailand and Malaysia in the ASEAN countries, especially in Thailand, where the insect is seriously harmful. The pest feeds on the old and new leaves of palm plants, causing the leaves of the plant to dry up, defoliation, and even the death of the entire plant in severe cases. In August 2013, the coconut weaver moth was first discovered in Wanning City, Hainan Province, my country, and has spread to Haikou, Danzhou, Baoting, Sanya, Qionghai, Wenchang and other cities and counties. According to the pest risk analysis (PRA) procedure stipulated by the International Standard for Phytosanitary Measures (ISPM), using the relevant risk analysis model, the qualitative and quantitative analysis of the risk of the coconut moth in Hainan Province and other provinces in China shows that: The risk R values of weaver moth in Hainan Province and other provinces in China were 2.37 and 2.09, respectively, which belonged to high-risk pests.

As a newly invasive pest, both basic biological research and production control require a large number of coconut moths as insect sources. Coconut weaver moth forms holes with insect feces on the back of the host leaf, and hides in them to do harm. In addition, palm plants such as coconuts are tall, so it is difficult to carry out chemical control. Foreign control experience shows that the release and utilization of natural enemies of the cocoa moth is the main measure to effectively control the cocoa moth. Most of the production of natural enemies of the cocoa moth is done with the cocoa moth as a host. Therefore, the large-scale reproduction technology of the cocoa moth is the basis for the development of biological control technology to control the reproduction of the cocoa moth by using natural enemies, and it is also the basis for carrying out other researches on the cocoa moth. At present, there is no research report on the large-scale breeding and rearing technology of coconut weaver moth at home and abroad.

Contents of the invention

The purpose of the present invention is to provide a method for mass propagation of the cocoa weaver moth, which has almost no damage to the cocoa weaver moth, low cost, high survival rate, and can obtain a large number of coco weaver moths with the same age.

The purpose of the present invention is achieved through the following technical solutions:

A method for mass propagation of coconut weaver moths, comprising the following steps:

(1) Breeding of adults of coconut weaver moth and induction of oviposition

a. the old leaves of the coconut tree are cut into small sections of 8 cm, put into a glass tube with a length of 8.5 cm and a diameter of 4.5 cm, and place 2 pieces in each glass tube;

b. Introduce a pair of newly hatched weaver moth adults into each of the glass tubes, use a cotton swab dipped in 10% honey water of mass concentration to supplement nutrition for the weaver moth adults, and seal the glass tubes with 200 mesh gauze;

c. After 1-2 days, the adults of the coconut weaver moth begin to lay eggs, and the eggs are laid on the surface of the leaves. Each female coconut weaver moth lays an average of 200 eggs. When the leaves are replaced every day, the leaves with eggs are picked out. Concentrate and put in another insect-raising box, and put 1000 eggs in each box;

(2) Egg hatching and rearing of 1st instar larvae

The eggs begin to hatch after 6-7 days. Before hatching, mix 10 pieces of 15cm-long old coconut leaves into the leaves with eggs. The newly hatched larvae will climb to the newly replaced leaves to feed, and replace them every 3 days. blade;

(3) Feeding of larvae and management of pupae

a. 1-3 instar larvae, feed 1000 per box, change the leaves every 4 days, mix the new leaves with the old leaves with insects, and pick the old leaves without insects and dead insects with mildew out;

b. 4-8 instar larvae, feed 200 larvae per box, replace the leaves every 4 days, mix the new leaves with the old leaves with worms, and pick the old leaves without worms and dead insects with mildew out;

c. The larval stage is 50 days. After 50 days, the larvae pupate. The pupal stage is 10 days. It is not necessary to replace the leaves, but the ambient humidity should be kept; Cotton swabs dipped in 10% honey water induced the adult moths to lay eggs and enter the next cycle of feeding and breeding.

In order to meet the healthy development of the cocoon weaver moth, the breeding conditions of each insect state are temperature 25-27 ℃, relative humidity 70%±10%, and natural light.

In order to improve the survival rate of the coconut weaver moth, the host used is the mature leaves of the coconut tree. Choose dark green leaves without disease spots and insect bites. The leaves are kept fresh and cannot be stained with water. Controlled in a freezer at 4°C, the storage period is up to 10 days.

Coconut leaves are held together with rubber bands to keep them hydrated, prolong their life, and provide shelter for the coco moth.

In order to increase the amount of eggs laid and the hatching rate, each glass tube was introduced with only one pair of newly emerged adults of the cocoa moth, and 10% honey was provided.

In order to prevent the degradation of the long-term breeding population, the wild coco moth population is replaced every year.

In order to produce intensively and meet the living space of the coconut weaver moth, the insect-raising box of the present invention is 25cm long, 15cm wide and 8cm high, and the lid center of the insect-raising box is provided with a rectangular mouth with a length of 11cm and a width of 4cm. Paste 200 mesh gauzes with glue; the insect raising box is placed on the insect raising frame, and the insect raising frame is made of universal angle steel, 2m long, 0.6m wide, and 1.8m high. Each iron frame is divided into four layers, the height of the bottom layer from the ground is 0.2m, and the distance between each layer is 0.4m. Each layer is equipped with a 40w fluorescent tube; four legs stand on a tray filled with water to prevent ants from invading the raised coconuts weaving moth.

In order to prevent the occurrence of epidemic diseases during the breeding process of coconut weaver moth, the insect breeding room is disinfected with 3% hydrogen peroxide spray every month; the breeding utensils are also regularly disinfected with 70% alcohol.

This rearing method is mainly used in mass production of each worm state of the coconut weaver moth.

The beneficial effect of the present invention is that: the present invention uses the old leaves of the coconut tree to raise the larvae of all ages, reduces the production cost, is simple and practical to operate, and can carry out continuous, balanced and batch breeding of the larvae of all ages of the coconut weaver moth. The average hatching rate of eggs of coconut weaver moth is 87%, the average survival rate of 1st instar larvae is 90%, the average survival rate of 2nd instar larvae is 92%, the average survival rate of 3rd instar larvae is 93%, and the survival rate of 4th instar larvae is The average survival rate of 5th instar larvae is 97%, the average survival rate of 6th instar larvae is 97%, the average survival rate of 7th instar larvae is 98%, the average survival rate of 8th instar larvae is 99%, and the average pupal eclosion rate is 99%. Raising coconut weaver moths with old coconut leaves is cheap and easy to obtain.

detailed description

Coconut weaver moth mass propagation method described in the embodiment of the present invention, comprises the following steps:

(1) Take old coconut leaves that have not been sprayed, have no disease spots and pest damage in the field, and put them in a freezer with a temperature controlled at 4°C, and they must not be stained with water. Open a 11cm long and 4cm wide opening in the center of the lid of the sealed plastic fresh-keeping box, and then seal it with 200 mesh gauze to make an insect breeding box; the insect raising box is placed on the insect raising frame, and the insect raising frame is made of universal angle steel Made, the insect rack is 2m long, 0.6m wide, and 1.8m high. Each insect rack is divided into four layers, the height of the bottom layer from the ground is 0.2m, and the distance between each layer is 0.4m. 40W fluorescent tube. The insect rack is placed in an insect culture room with a temperature of 25-27°C, a relative humidity of 70%±10%, and natural light;

(2), cut the old leaves of the coconut tree into small sections of 8 cm, put them into a glass tube with a length of 8.5 cm and a diameter of 4.5 cm, then introduce a pair of newly hatched adults of the coconut moth, and use a glass tube dipped in 10% honey water Cotton swabs supplement nutrition for the coconut weaver moth adults, allowing them to feed and lay eggs. After 1-2 days, the coconut weaver moth begins to lay eggs. The eggs are laid on the surface of the leaves. The leaves are replaced once a day. out, put the same batch into another insect breeding box, and put 1000 eggs in each box;

(3) The eggs start to hatch after 6-7 days, and the humidity of the environment should be maintained during the period; before hatching, mix about 10 pieces of old coconut leaves with a length of 15cm into the leaves with eggs, and the newly hatched larvae will automatically climb to the old leaves Feed and develop from above; take out the newly hatched larvae, and put the same batch into the same insect rearing box; the first instar larvae period is about 6 days, and the old leaves of the coconut tree are replaced every 3 days;

(4) The 2nd instar larvae period of the coconut weaver moth is about 6 days, the 3rd instar larvae period is about 7 days, the 4th instar larvae period is about 7 days, the 5th instar larvae period is about 5 days, and the 6th instar larvae period is about 5 days days, the 7th instar larvae period is about 6 days, the 8th instar larvae period is about 8 days, and the larvae period is 50 days in total; according to the food intake, 1-3 instar larvae can feed 1,000 heads per box; 4-8 instar larvae, Each box can raise 200 heads; during the period, keep the environmental humidity and the leaves without water loss, and pay attention to the occurrence of coconut weaver moth epidemics. The insect breeding room is disinfected with 3% hydrogen peroxide spray every month; the breeding utensils are also regularly disinfected with 70% alcohol;

(5) After 50 days, the coconut moth enters the pupal stage, which lasts about 10 days. During this period, the leaves do not need to be replaced, but the ambient humidity must be maintained. After the pupae emerge into adults, they are paired and placed in a glass tube, and new leaves are replaced. And add the cotton swab that is dipped in 10% honey water, allow it to feed and lay eggs, enter the raising process of next cycle.

The larvae of the coconut weaver moth mass propagation method described in the embodiments of the present invention are judged based on the width of the peeled head shell at each instar.

As shown in Table 1, the breeding of the coconut weaver moth mass propagation method described in the embodiments of the present invention is through the test results.

Table 1 Feeding effect of each insect state of coconut weaver moth

According to the results obtained in Table 1, it can be concluded that the hatching rate of coconut weaver moth eggs is 87% on average, the survival rate of 1st instar larvae is 90% on average, the survival rate of 2nd instar larvae is 92% on average, and the 3rd instar larvae The average survival rate of larvae is 93%, the average survival rate of 4th instar larvae is 95%, the average survival rate of 5th instar larvae is 97%, the average survival rate of 6th instar larvae is 97%, the average survival rate of 7th instar larvae is 98%, and the average survival rate of 8th instar larvae is 98%. The average larval survival rate was 99%, and the average pupal emergence rate was 99%.

The present invention is not limited to the above-mentioned best implementation mode, anyone can draw other various forms of products under the inspiration of the present invention, but no matter make any changes in its shape or structure, all those with the same or similar features as the present application Approximate technical solutions all fall within the protection scope of the present invention.

Claims (9)

1. a method for mass propagation of coconut weaver moth, is characterized in that, comprises the following steps: (1) Breeding of coconut weaver moth adults and inducing their oviposition: a. Cut the old leaves of the coconut tree into small sections of 8 cm, put them into glass tubes, and place 2 pieces in each glass tube; B. Introduce a pair of newly hatched weaver moth adults into each glass tube, and use a cotton swab dipped in honey water with a mass concentration of 10% to supplement nutrition for the weaver moth adults, and the glass tubes are sealed with 200 mesh gauze; c. After 1-2 days, the adults of the coconut weaver moth begin to lay eggs, and the eggs are laid on the surface of the leaves. Each female coconut weaver moth lays an average of 200 eggs. When the leaves are replaced every day, the leaves with eggs are picked out. Concentrate and put in another insect-raising box, and put 1000 eggs in each box; (2) Egg hatching and rearing of 1st instar larvae: The eggs begin to hatch after 6-7 days. Before hatching, mix 10 pieces of 15cm-long old coconut leaves into the leaves with eggs. The newly hatched larvae will climb to the newly replaced leaves to feed, and replace them every 3 days. blade; (3) Feeding of larvae and management of pupae: a. 1-3 instar larvae, feed 1000 per box, change the leaves every 4 days, mix the new leaves with the old leaves with insects, and pick the old leaves without insects and dead insects with mildew out; b. 4-8 instar larvae, feed 200 larvae per box, replace the leaves every 4 days, mix the new leaves with the old leaves with worms, and pick the old leaves without worms and dead insects with mildew out; c. The larval stage is 50 days, and after 50 days, the larvae pupate, and the pupal stage is 10 days, without changing the leaves, but keeping the ambient humidity; after the pupae emerge into adults, pair them into the glass tube, add the leaves and Cotton swabs dipped in 10% honey water induced the adult moths to lay eggs, and entered the next cycle of feeding and breeding. The feeding conditions of each worm state were temperature 25-27°C, relative humidity 70%±10%, and natural light. 2. The mass propagation method of the coconut weaver moth according to claim 1, characterized in that: the host of the coconut weaver moth is a scattered mature coconut leaf, and the leaves remain bright green and do not lose water. 3. The mass propagation method of the coconut weaver moth according to claim 1, characterized in that: when the larvae are replaced, new leaves are used to mix the old leaves with worms, and they are tied together with rubber bands, but should not be too tight. 4. method for large-scale propagation of coconut weaver moth according to claim 1, is characterized in that: described glass tube length is 8.5cm, and diameter is 4.5cm, only puts a pair of coconut weaver moth adults just eclosion in every glass tube , and provide 10% honey. 5. The method for mass breeding of coconut weaver moths according to claim 1, characterized in that: the insect breeding room needs to be kept clean and disinfected with 3% hydrogen peroxide spray every month; the breeding utensils should also be regularly disinfected with 70% alcohol. 6. The method for mass propagation of the coconut weaver moth according to claim 1, characterized in that: the obtained old leaves of the coconut tree need to be stored in a freezer with a temperature controlled at 4°C. 7. method for mass breeding of coconut weaver moth according to claim 1, is characterized in that: the length of the insect-raising box is 25cm, the width is 15cm, and the height is 8cm; It is 11cm, and the width is the rectangular mouth of 4cm, and described rectangular mouth is provided with 200 order gauzes. 8. method for massive breeding of coconut weaver moth according to claim 1, is characterized in that: described insect-raising box is placed on the insect-raising frame, and described insect-raising frame is made of universal angle steel, and the length of insect-raising frame is 2m, The width is 0.6m and the height is 1.8m. The insect rack is divided into four layers. The height of the bottom layer from the ground is 0.2m, and the distance between each layer is 0.4m. Stand on four feet on a tray filled with water. 9. The mass propagation method of the weaver moth according to claim 1, characterized in that: the adults of the weaver moth are collected once a year from the field, and all the original species are replaced.