CN103766288B - The a large amount of propagation method of moth knitted by coconut - Google Patents
The a large amount of propagation method of moth knitted by coconut Download PDFInfo
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- CN103766288B CN103766288B CN201410024567.2A CN201410024567A CN103766288B CN 103766288 B CN103766288 B CN 103766288B CN 201410024567 A CN201410024567 A CN 201410024567A CN 103766288 B CN103766288 B CN 103766288B
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Abstract
The present invention relates to a kind of coconut and knit a large amount of propagation method of moth, in temperature 25��27 DEG C, relative humidity 75% �� 10%, when natural lighting, carry out the raising that each worm state of moth knitted by coconut. Just incubate coconut and knit the pairing of moth male and female, introduce in the Glass tubing with coconut Lao Ye, and provide 10% hydromel to supply nutrient; After 1-2 days, coconut knits moth egg in coconut blade; The blade having ovum is chosen, concentrates and put into an insect box, every box 1000 ovum; Ovum can be hatched as larva after spending 6-7 days, within every 4 days, changes primary vane; Pupating after 50 days, after 9-12 days, pupa turns into adult, enters the captive breeding process of next circulation. The present invention is with cocoanut tree Lao Ye breeding grub, and practicality easy and simple to handle, can carry out continuously, balanced, batch breeding coconut knit each instar of moth, knit the fundamental research of moth for coconut and prevention and control provide a large amount of worm source.
Description
Technical field
The present invention relates to the raising of a kind of insect and propagation method, it is specifically related to a kind of coconut and knits a large amount of propagation method of moth.
Background technology
Moth (OpisinaarenosellaWallker) knitted by coconut is that external harmful organism is newly invaded by China, belong to lepidopteran (Lepidoptera), Zhi E section (Oecophoridae), moth English coconutblackheadedcaterpillar by name knitted by coconut, and Chinese translation has coconut blackhead crawler belt worm, food leaf crawler belt worm and coconut palm moth moth. Moth knitted by coconut is the important pests on palm plant. In India, Sri Lanka and Burma, coconut is knitted moth and is seriously endangered coconut palm. Coconut was knitted moth and had been spread the Thailand and Malaysia that spread to ASEAN countries in recent years, and especially in Thailand, the harm of this worm is serious. This insect takes food palm plant Lao Ye and Xin Ye, causes plant leaves to dry up, fallen leaves occurs, and whole strain can be caused time serious dead. In August, 2013, coconut knits moth at China's Behavioral Change Late Cambrian, and has been diffused into the cities and counties such as Haikou, Danzhou, Bao Ting, Sanya, Qionghai, Wenchang. According to pest risk analysis (PRA) program that international Plant Quarantine Measures Standard (ISPM) specifies, utilize relevant risk analytical model, coconut is knitted moth carry out qualitative and quantitative analysis at the risk in Hainan Province and other provinces domestic and show: coconut is knitted the dangerous R value of moth in Hainan Province and other provinces domestic and is respectively 2.37 and 2.09, belongs to excessive risk harmful organism.
All need a large amount of coconuts to knit moth as the prevention and control in the insect of new invasion, fundamental biological knowledge research or production and do worm source. Coconut is knitted moth and is become duct at host's leaf back worm coproma, hides and endangers wherein, and the palm plant such as coconut is tall and big in addition, it is difficult to carry out chemical prevention. External experience of prevention and treatment shows, the release utilization of the natural enemy of moth knitted by coconut is effectively control the major measure that moth knitted by coconut. The production that moth natural enemy knitted by coconut is mostly knitted moth with coconut and is completed as host. Therefore, a large amount of breeding technologys of moth knitted by coconut is the basis that the biological prevention of moth breeding knitted by application natural enemy control coconut, is also carry out the basis that moth other side research knitted by coconut. There is no the research report knitting a large amount of propagating and breeding technology of moth about coconut at present both at home and abroad.
Summary of the invention
It is an object of the invention to provide a kind of coconut and knit a large amount of propagation method of moth, coconut is knitted moth and does not almost injure, low cost, survival rate height, a large amount of worm age consistent coconut can be obtained and knit moth.
It is an object of the invention to be achieved through the following technical solutions:
The a large amount of propagation method of moth knitted by a kind of coconut, comprises the following steps:
(1) coconut is knitted the raising of moth adult and is brought out it and lays eggs
A. the segment that cocoanut tree Lao Ye is cut into 8cm, to put into length be 8.5cm, diameter is the Glass tubing of 4.5cm, places 2 in each Glass tubing;
B. in Glass tubing described in each introduce one to just incubating coconut knit moth adult, be that moth Adult supplement nutrient knitted by coconut with the cotton swab being moistened with mass concentration 10% hydromel, described Glass tubing seals with 200 order yarn nets;
C.1-2 behind sky, coconut is knitted moth adult and is started to lay eggs, ovum be scheduling in blade surface, often only moth adult eggs on average 200 knitted by female coconut, is chosen by the blade with ovum when every day changes blade, concentrates for same batch and puts into another insect box, and every box puts 1000 ovum;
(2) raising of egg hatching and 1 instar larvae
Ovum started hatching after 6-7 days, was mixed in the blade with ovum by the cocoanut tree Lao Ye of 10 15cm length before hatching, and newly hatched larvae can be climbed to voluntarily and take food on the blade of new replacing, within every 3 days, changes primary vane;
(3) larval stage, raises the management with pupa
A.1-3 instar larvae, every box raises 1000, within every 4 days, changes 1 blade, new leaf is mixed in the Lao Ye being placed on band worm, chosen by the dead worm of the Lao Ye of not band worm and band mildew;
B.4-8 instar larvae, every box raises 200, within every 4 days, changes 1 blade, new leaf is mixed in the Lao Ye being placed on band worm, chosen by the dead worm of the Lao Ye of not band worm and band mildew;
C. larval stage 50 days, larvae pupation after 50 days, pupa time is 10 days, does not need to change blade, but to be kept ambient moisture; After pupa turns into adult, carry out pairing and put into described Glass tubing, add blade and knit moth Adult worms producting eggs with the cotton swab induction coconut being moistened with 10% hydromel, enter the captive breeding of next circulation.
Knit the healthy development of moth to meet coconut, each worm state rearing conditions is temperature 25��27 DEG C, relative humidity 70% �� 10%, natural lighting.
Knit the survival rate of moth to improve coconut, the mature leaf that host used launches for cocoanut tree, select blackish green and without the leaf of scab and insect bite, blade keeps fresh, and moisture can not be speckled with, cocoanut tree Lao Ye can leave temperature in and control in the refrigerator-freezer of 4 DEG C, and staging life reaches 10 days.
In order to keep cocoanut tree leaf moisture, extend work-ing life, and providing coconut to knit the hidden living environment of moth, coconut leaf bungee bands together.
In order to improve egg laying amount and hatching rate, often prop up Glass tubing and only introduce one the coconut just sprouted wings is knitted moth adult, and 10% honey is provided.
In order to prevent Long-term breeding population deterioration, moth population knitted by the wild coconut of annual replacing.
In order to intensive manufacture, and meeting the living space that moth knitted by coconut, the long 25cm of the insect box of the present invention, wide 15cm, high 8cm, it is 11cm, the wide rectangle hole for 4cm that the lid central authorities at insect box are provided with long, pastes upper 200 object yarn nets with glue; Insect box is placed on foster worm frame, and described foster worm frame is made up of omnipotent angle steel, long 2m, wide 0.6m, high 1.8m. Each brandreth divides four layers, and lowest layer terrain clearance is 0.2m, every interlamellar spacing 0.4m, is furnished with 1 40w fluorescent tube for every layer; Four legs stand on the pallet being filled with water, and moth knitted by the coconut preventing ant infringement from raising.
In order to prevent coconut from knitting the generation of popular disease in moth feeding process, insectary is monthly with 3% hydrogen peroxide spraying disinfection; Breeding facility also regularly uses 70% alcohol disinfecting.
This method for breeding is mainly used in batch production coconut and knits each worm state of moth.
The invention has the beneficial effects as follows: the present invention raises each instar larvae with cocoanut tree Lao Ye, reduces production cost, and practicality easy and simple to handle, can carry out continuously, balanced, batch breeding coconut knit each instar larvae of moth. The hatching rate of moth ovum knitted by coconut is on average 87%, the survival rate of 1 instar larvae is on average 90%, the survival rate of 2 instar larvaes is on average 92%, the survival rate of 3 instar larvaes is on average 93%, 4 instar larvae survival rates be on average 95%, 5 instar larvae survival rates is on average 97%, 6 instar larvae survival rates are on average 97%, 7 instar larvae survival rates are on average 98%, 8 instar larvae survival rates is on average 99%, and pupa eclosion rate is on average 99%. Utilize coconut Lao Ye to raise coconut and knit moth, with low cost, it is easy to obtain.
Embodiment
The a large amount of propagation method of moth knitted by coconut described in the embodiment of the present invention, comprises the following steps:
(1), field take not spray medicine, without scab and insect infringement cocoanut tree Lao Ye, be placed on temperature and control in the refrigerator-freezer of 4 DEG C, and moisture can not be speckled with, for subsequent use; Lid central authorities at the sealed plastic crisper of long 25cm, wide 15cm, high 8cm open the mouth of a long 11cm, wide 4cm, then seal with 200 object yarn nets, make insect box; Insect box is placed on foster worm frame, supports worm frame and is made up of omnipotent angle steel, support worm frame length be 2m, wide for 0.6m, high be 1.8m, each foster worm frame divides four layers, and lowest layer terrain clearance is 0.2m, every interlamellar spacing 0.4m, is furnished with the fluorescent tube of 1 40W for every layer. Foster worm is placed on that temperature is 25-27 DEG C, relative humidity is 70% �� 10%, in the insectary of natural lighting;
(2), cocoanut tree Lao Ye is cut into the segment of 8cm, to put into length be 8.5cm, diameter is the Glass tubing of 4.5cm, then coconut is knitted and is incubated adult at the beginning of moth by introducing 1, is that moth Adult supplement nutrient knitted by coconut with the cotton swab being moistened with 10% hydromel, allows it take food and lay eggs, after 1-2 days, coconut is knitted moth and is started to lay eggs, ovum be scheduling in blade surface, every day changes primary vane, change blade time the blade with ovum is chosen, concentrating for same batch and put into another insect box, every box puts 1000 ovum;
(3), ovum started after 6-7 days hatching, period to be kept the humidity of environment; Before hatching, the cocoanut tree Lao Ye of about 10 15cm length being mixed into the blade with ovum, newly hatched larvae can automatically be climbed to and take food growth on Lao Ye; To have newly hatched larvae to take out, and concentrate for same batch and put into same insect box; About 6 days of 1 instar larvae phase, within every 3 days, change 1 old blade of cocoanut tree;
(4), coconut knit the moth 2 instar larvae phase and be about 6 days, 3 instar larvae phases were about 7 days, and 4 instar larvae phases were about 7 days, and 5 instar larvae phases were about 5 days, and 6 instar larvae phases were about 5 days, and 7 instar larvae phases were about 6 days, and 8 instar larvae phases were about 8 days, and larval stage amounts to 50 days; According to food ingestion, 1-3 instar larvae, every box can raise 1000; 4-8 instar larvae, every box can raise 200; Period to be kept ambient moisture and blade not dehydration, is also noted that coconut is knitted moth prevailing disease and occurred, and insectary is monthly with 3% hydrogen peroxide spraying disinfection; Breeding facility also regularly uses 70% alcohol disinfecting;
(5), after 50 days, coconut is knitted moth and is entered pupa time, about 10 days of pupa time, period does not need to change blade, but to be kept ambient moisture, after pupa turns into adult, carry out pairing and put into Glass tubing, the blade that more renews also adds the cotton swab being moistened with 10% hydromel, allows it take food and lays eggs, and enters the feeding process of next circulation.
Coconut described in the embodiment of the present invention knits larva each length of time of a large amount of propagation method of moth taking head capsule width of peeling as basis for estimation.
The raising that a large amount of propagation method of moth knitted by coconut described in the embodiment of the present invention is as shown in table 1 through test-results.
The each worm state feeding effect of moth knitted by table 1 coconut
According to the result that table 1 draws, can reach a conclusion: the hatching rate of moth ovum knitted by coconut is on average 87%, the survival rate of 1 instar larvae is on average the survival rate of 90%, 2 instar larvaes be on average the survival rate of 92%, 3 instar larvaes is on average 93%, 4 instar larvae survival rates are on average 95%, 5 instar larvae survival rates are on average 97%, 6 instar larvae survival rates be on average 97%, 7 instar larvae survival rates is on average 98%, 8 instar larvae survival rates are on average 99%, and pupa eclosion rate is on average 99%.
The present invention is not limited to above-mentioned preferred forms; anyone can draw the product of other various forms under the enlightenment of the present invention; no matter but in its shape or structure, do any change; every have the technical scheme identical or close with the application, all drops within protection scope of the present invention.
Claims (9)
1. a large amount of propagation method of moth knitted by a coconut, it is characterised in that, comprise the following steps:
(1) coconut is knitted the raising of moth adult and is brought out it and lays eggs:
A., the segment that cocoanut tree Lao Ye is cut into 8cm, puts into Glass tubing, places 2 in each Glass tubing;
B. in each Glass tubing introduce one to just incubating coconut knit moth adult, be that moth Adult supplement nutrient knitted by coconut with the cotton swab of the hydromel being moistened with mass concentration 10%, described Glass tubing seals with 200 order yarn nets;
C.1-2 behind sky, coconut is knitted moth adult and is started to lay eggs, ovum be scheduling in blade surface, often only moth adult eggs on average 200 knitted by female coconut, is chosen by the blade with ovum when every day changes blade, concentrates for same batch and puts into another insect box, and every box puts 1000 ovum;
(2) raising of egg hatching and 1 instar larvae:
Ovum started hatching after 6-7 days, was mixed in the blade with ovum by the cocoanut tree Lao Ye of 10 15cm length before hatching, and newly hatched larvae can be climbed to voluntarily and take food on the blade of new replacing, within every 3 days, changes primary vane;
(3) larval stage, raises the management with pupa:
A.1-3 instar larvae, every box raises 1000, within every 4 days, changes 1 blade, new leaf is mixed in the Lao Ye being placed on band worm, chosen by the dead worm of the Lao Ye of not band worm and band mildew;
B.4-8 instar larvae, every box raises 200, within every 4 days, changes 1 blade, new leaf is mixed in the Lao Ye being placed on band worm, chosen by the dead worm of the Lao Ye of not band worm and band mildew;
C. larval stage 50 days, larvae pupation after 50 days, pupa time is 10 days, does not need to change blade, but to be kept ambient moisture; After pupa turns into adult, carry out pairing and put into described Glass tubing, add blade and knit moth Adult worms producting eggs with the cotton swab induction coconut being moistened with 10% hydromel, enter the captive breeding of next circulation, each worm state rearing conditions is temperature 25��27 DEG C, relative humidity 70% �� 10%, natural lighting.
2. a large amount of propagation method of moth knitted by coconut according to claim 1, it is characterised in that: the host of moth knitted by described coconut is the ripe coconut leaf scattered, and blade keeps bud green and not dehydration.
3. a large amount of propagation method of moth knitted by coconut according to claim 1, it is characterised in that: larva raising, when changing blade, utilizes new leaf mixed zone worm Lao Ye, and utilizes bungee to band together, but should not be excessively tight.
4. a large amount of propagation method of moth knitted by coconut according to claim 1, it is characterised in that: described Glass tubing length is 8.5cm, and diameter is 4.5cm, often props up and only puts one in Glass tubing the coconut just sprouted wings is knitted moth adult, and provides 10% honey.
5. a large amount of propagation method of moth knitted by coconut according to claim 1, it is characterised in that: insectary need to keep clean, monthly with 3% hydrogen peroxide spraying disinfection; Breeding facility also regularly to be used 70% alcohol disinfecting.
6. a large amount of propagation method of moth knitted by coconut according to claim 1, it is characterised in that: the cocoanut tree Lao Ye obtained need to be stored in temperature and control in the refrigerator-freezer of 4 DEG C.
7. a large amount of propagation method of moth knitted by coconut according to claim 1, it is characterized in that: described insect box length be 25cm, wide for 15cm, high be 8cm, it is 11cm, the wide rectangle hole for 4cm that lid central authorities at described insect box are provided with long, and described rectangle hole is provided with 200 object yarn nets.
8. a large amount of propagation method of moth knitted by coconut according to claim 1, it is characterized in that: described insect box is placed on foster worm frame, described foster worm frame is made up of omnipotent angle steel, support worm frame length be 2m, wide for 0.6m, high be 1.8m, supporting worm frame and divide four layers, lowest layer terrain clearance is 0.2m, and every interlamellar spacing is 0.4m, every layer is furnished with 1 power is the fluorescent tube of 40W, and four legs supporting worm frame stand on the pallet being filled with water.
9. a large amount of propagation method of moth knitted by coconut according to claim 1, it is characterised in that: knit moth adult from field acquisition coconut every year, all change original seed worm.
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| CN104186430B (en) * | 2014-08-14 | 2016-06-29 | 中国热带农业科学院椰子研究所 | The method of the laboratory test population mass rearing of moth knitted by Cortex cocois radicis |
| CN107410217B (en) * | 2017-05-16 | 2023-06-06 | 中国热带农业科学院环境与植物保护研究所 | Breeding device and method for experimental population of cassava single-claw mites |
| CN112471079B (en) * | 2020-12-11 | 2022-02-01 | 兰州大学 | Indoor artificial feeding method for Qinghai-Tibet plateau grassland caterpillars |
| CN112772573B (en) * | 2021-01-27 | 2022-09-16 | 广西壮族自治区农业科学院植物保护研究所 | Indoor artificial breeding method for acrossodes amabilis |
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