CN105145495A - Artificial breeding method for jujube tree porthesia similis - Google Patents
Artificial breeding method for jujube tree porthesia similis Download PDFInfo
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- CN105145495A CN105145495A CN201510573938.7A CN201510573938A CN105145495A CN 105145495 A CN105145495 A CN 105145495A CN 201510573938 A CN201510573938 A CN 201510573938A CN 105145495 A CN105145495 A CN 105145495A
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- 238000009395 breeding Methods 0.000 title claims abstract description 26
- 244000126002 Ziziphus vulgaris Species 0.000 title claims abstract description 23
- 235000008529 Ziziphus vulgaris Nutrition 0.000 title claims abstract description 17
- 241000483002 Euproctis similis Species 0.000 title abstract description 7
- 239000002689 soil Substances 0.000 claims abstract description 25
- 231100000614 poison Toxicity 0.000 claims description 59
- 239000002574 poison Substances 0.000 claims description 59
- 102000002322 Egg Proteins Human genes 0.000 claims description 15
- 108010000912 Egg Proteins Proteins 0.000 claims description 15
- 210000004681 ovum Anatomy 0.000 claims description 15
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 claims description 9
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- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 claims description 9
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- 238000005266 casting Methods 0.000 claims description 6
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- 235000013601 eggs Nutrition 0.000 abstract description 7
- 239000003053 toxin Substances 0.000 abstract description 4
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- 239000002435 venom Substances 0.000 description 1
- 231100000611 venom Toxicity 0.000 description 1
- 210000001048 venom Anatomy 0.000 description 1
- 235000019786 weight gain Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention discloses an artificial breeding method for jujube tree porthesia similis. The method includes the specific steps that porthesia similis eggs are placed in a raising box to be incubated; after the porthesia similis eggs are incubated into larvae, feed is placed into the raising box, and the top of the raising box is blocked by a black-out cloth; the temperature and humidity of the interior of the raising box are recorded in the morning and evening each day, the replacing frequency of the feed is 3-5 each day, and raising is conducted; the death number, the ecdysis frequency and the cocooning number of the larvae are counted every two days, the cocooned larvae on the same day are placed in a raising box with wet soil of 3-5 mm laid on the bottom, and the top of the raising box is blocked by a black-out cloth until the porthesia similis is emerged into adults. According to the method, jujube tree leaves, euonymus bungeanus leaves and cabbage leaves which can be obtained easily are used as feed, the artificial breeding method is adopted, large-scale propagation of the porthesia similis is achieved, and a foundation is provided for development and utilization of new toxin; moreover, target spot test insects can be provided for study of pharmacology and the like.
Description
Technical field
The invention belongs to technical field of insect feeding, be specifically related to the artificial breeding method that a kind of jujube tree steals poison moth.
Background technology
Steal poison moth (Porthesiasimilis) and have another name called mulberry poison moth, yellow tail poison moth, belong to Lepidoptera (Lepidoptera) Lymantriidae (Lymantriidae), be a kind of polyphagy insect, be domesticly mainly distributed in northeast, North China, northwest and Central China; Mainly be distributed in Japan, the Korea peninsula and Russia abroad.Stealing poison moth can the multiple shrub such as serious harm oak class, Chinese ash, willow, Chinese parasol tree, hawthorn, apple, mulberry, oil tea, serious can by leaf eat everything up when occurring; But steal poison moth whole body tool many places poison gland, human contact, to the malicious hair stolen on poison moth, can cause dermatitis, have cause lymph inflammation (kingdom's profit etc. Gansu Agriculture University's journal, 2011,04:53-56; Fan Changmi. northwest gardening (fruit tree), 2011,04:29-30).Therefore breeding in enormous quantities is conducive to it gathers venom in a large number, for toxicity, pharmaceutical research, and be conducive to the development and utilization of novel toxin medicine, preparation.Meanwhile, relative to other laboratory animal, steal the feeding cost that poison moth is lower, target spot can be provided to try worm for pharmaceutical research.
Summary of the invention
The object of the present invention is to provide a kind of jujube tree to steal the artificial breeding method of poison moth, cultivated by the method for artificial breeding and steal poison moth in enormous quantities, for novel toxin development and utilization provides basis, and target spot can be provided to try worm for researchs such as pharmacology.
The technical solution adopted in the present invention is, jujube tree steals the artificial breeding method of poison moth, specifically implements according to the following steps:
Step 1: robber poison moth ovum is placed on raise in box and hatches;
Step 2: after robber poison moth egg hatching becomes larva, put into feed in raising box, and by the gobo shutoff of its top; Every day, sooner or later temp. and humidity in box raised in record, and the replacing speed of feed is every day 3 ~ 5 times, raises;
Step 3: every two days statistics dead larvae number, number of times of casting off a skin, a number of cocooing, and be covered with in the raising box of 3 ~ 5mm wet soil bottom the larva cocoond on the same day is placed into, the gobo shutoff of its top, until steal poison moth emerging adult, to obtain final product.
Feature of the present invention is also,
In step 1, the bottom of raising box is equipped with the wet soil that thickness is 2 ~ 5mm.
Wet soil is through ultra violet lamp 30 ~ 50min gained.
In step 2, feed is the one in silk flosssilk wadding wood leaf, jujube leaf or Chinese cabbage leaf.
In step 2, raising temperature in box is 26 ~ 30 DEG C, and relative moisture is 60 ~ 70%.
Gained adult is placed on bottom and is equipped with captive breeding in the open-top receptacle of 1 ~ 2mm wet soil; Be placed with in open-top receptacle and steal poison moth ovum collecting rod and nutritional supplementation liquid.
Steal hardboard or branch bar that poison moth ovum collecting rod is monolayer medical gauze parcel.
Nutritional supplementation liquid to be concentration be 10% syrup or 3% hydromel.
The invention has the beneficial effects as follows, utilize the jujube leaf, silk flosssilk wadding wood leaf and the Chinese cabbage leaf that easily obtain as feed, adopt artificial breeding method, achieve the large-scale breeding stealing poison moth, for novel toxin development and utilization provides basis, and target spot can be provided to try worm for researchs such as pharmacology.
Accompanying drawing explanation
Fig. 1 is the long variation diagram of body of the robber poison moth that the embodiment of the present invention 1 is bred;
Fig. 2 is the body weight change figure of the robber poison moth that the embodiment of the present invention 1 is bred;
Fig. 3 is the long variation diagram of body of the robber poison moth that the embodiment of the present invention 2 is bred;
Fig. 4 is the body weight change figure of the robber poison moth that the embodiment of the present invention 2 is bred;
Fig. 5 is the long variation diagram of body of the robber poison moth that the embodiment of the present invention 3 is bred;
Fig. 6 is the body weight change figure of the robber poison moth that the embodiment of the present invention 3 is bred.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention is described in detail.
Jujube tree of the present invention steals the artificial breeding method of poison moth, specifically implements according to the following steps:
Step 1: robber poison moth ovum is placed on raise in box and hatches;
Step 2: after robber poison moth egg hatching becomes larva, put into feed in raising box, and by the gobo shutoff of its top; Every day, sooner or later temp. and humidity in box raised in record, and the replacing speed of feed is every day 3 ~ 5 times, raises;
Step 3: every two days statistics dead larvae number, number of times of casting off a skin, a number of cocooing, and be covered with in the raising box of 3 ~ 5mm wet soil bottom the larva cocoond on the same day is placed into, the gobo shutoff of its top, until steal poison moth emerging adult, to obtain final product.Wherein:
In step 1, the bottom of raising box is equipped with the wet soil that thickness is 2 ~ 5mm.
Wet soil is through ultra violet lamp 30 ~ 50min gained.
In step 2, feed is the one in silk flosssilk wadding wood leaf, jujube leaf or Chinese cabbage leaf.
In step 2, raising temperature in box is 26 ~ 30 DEG C, and relative moisture is 60 ~ 70%.
Gained adult is placed on bottom and is equipped with captive breeding in the open-top receptacle of 1 ~ 2mm wet soil; Be placed with in open-top receptacle and steal poison moth ovum collecting rod and nutritional supplementation liquid.Wherein, hardboard or branch bar that poison moth ovum collecting rod is monolayer medical gauze parcel is stolen; Nutritional supplementation liquid to be concentration be 10% syrup or 3% hydromel.
Embodiment 1
With silk flosssilk wadding wood leaf for poison moth is stolen in feed breeding:
Step 1, gets the crisper of 0.6L, is used for moisturizing by spreading the soil layer being about 2-5mm bottom it, to place it under uviol lamp sterilizing 30 minutes.
Step 2, will be divided into six zonules bottom the crisper in step 1, each region inserts 2-3 sheet fresh silk flosssilk wadding wood leaf, the water moisturizing that uniform sprinkling is appropriate.
Step 3, covers with black cloth after putting into larva and is being tightened by mouth with rubber band.
Step 4, every day is recording room temp. and humidity sooner or later, and controlling its temperature is 26 ~ 30 DEG C, and relative moisture is 60 ~ 70%, and changes silk flosssilk wadding wood leaf in time.
Step 5, every two days statistics once dead number, number of times of casting off a skin, number of cocooing.The larva cocoond on the same day being placed into bottom is covered with in the crisper of 3-5mm wet soil, hides rubber band sealing with black cloth.
Step 6, collects with the UP pipe of 3-5ml after turning into adult, before being placed into closed window during collection, and single only collection.
Step 7, after adult enters UP pipe, pins the mouth of pipe with thumb rapidly, then puts into rapidly the beaker of 1000ml.
Step 8, beaker bottom spreads the wet soil of about 1-2mm, and the hardboard distance rim of a cup entering to be wrapped with hospital gauze (individual layer) at beaker oblique cutting is about 1.5cm (be used for collecting and steal poison moth ovum).
Step 9, puts into the sterilized penicillin bottle of (or hydromel of 3%) that 10% syrup is housed at the another side of beaker, puts into absorbent cotton or filter paper exceeds bottle 2-3cm in this penicillin bottle.
Step 10, beaker mouth black cloth hides rubber band sealing.
Step 11, after Adult worms producting eggs, changes gauze in time.
Step 12, hatches the crisper that the wet soil being covered with 1-2mm put into by the gauze with ovum.Second generation larva after hatching treats that next time, experiment used.
The long variation diagram of body of the robber poison moth that the present embodiment is bred as shown in Figure 1; Steal the body weight change of poison moth as shown in Figure 2; Silk flosssilk wadding wood leaf is that diet steals poison moth as can be seen from Figure, and its body weight gain significantly increases in raising for 9 days afterwards.
Embodiment 2
With jujube leaf for poison moth is stolen in feed breeding:
Step 1, gets the crisper of 0.6L, is used for moisturizing by spreading the soil layer being about 2-5mm bottom it, to place it under uviol lamp sterilizing 30 minutes.
Step 2, will be divided into six zonules bottom the crisper in step 1, each region inserts the fresh jujube tree leaf of 2-3 sheet, the water moisturizing that uniform sprinkling is appropriate.
Step 3, covers crisper mouth with black cloth after putting into larva, is tightened by mouth with rubber band.
Step 4, every day is recording room temp. and humidity sooner or later, and controlling its temperature is 26 ~ 30 DEG C, and relative moisture is 60 ~ 70%, and changes jujube leaf in time.
Step 5, every two days statistics once dead number, number of times of casting off a skin, number of cocooing.The larva cocoond on the same day being placed into bottom is covered with in the crisper of 3-5mm wet soil, hides the sealing of sturdy pines band with black cloth.
Step 6, collects with the UP pipe of 3-5ml after turning into adult, before being placed into closed window during collection, and single only collection.
Step 7, after adult enters UP pipe, pins the mouth of pipe with thumb rapidly, then puts into rapidly the beaker of 1000ml.
Step 8, beaker bottom spreads the wet soil of about 1-2mm, and the hardboard distance rim of a cup entering to be wrapped with hospital gauze (individual layer) at beaker oblique cutting is about 1.5cm (be used for collecting and steal poison moth ovum).
Step 9, puts into the sterilized penicillin bottle of (or hydromel of 3%) that 10% syrup is housed at the another side of beaker, puts into absorbent cotton or filter paper exceeds bottle 2-3cm in this penicillin bottle.
Step 10, beaker mouth black cloth hides the sealing of sturdy pines band.
Step 11, after Adult worms producting eggs, changes gauze in time.
Step 12, hatches the crisper that the wet soil being covered with 1-2mm put into by the gauze with ovum.Second generation larva after hatching treats that next time, experiment used.
The long variation diagram of body of the robber poison moth that the present embodiment is bred as shown in Figure 3; Steal the body weight change of poison moth as shown in Figure 4; As can be seen from Figure with jujube leaf for diet steals poison moth, 18 days precursors are long to be increased rapidly, and body weight then slowly increases; Be in the stage of stable development gradually along with time lengthening body is long, body weight is then in weightening finish fast.
Embodiment 3
With Chinese cabbage leaf for poison moth is stolen in feed breeding:
Step 1, by commercial Chinese cabbage leaf clean water 2-3 time, stand-by.
Step 2, gets the crisper of 0.6L, is used for moisturizing by spreading the soil layer being about 2-3mm bottom it, to place it under uviol lamp sterilizing 30 minutes.
Step 3, is divided into six zonules bottom box, each region keeps flat the fresh Chinese cabbage leaf of 2-3 sheet.
Step 4, covers with black cloth after putting into larva and is being tightened by mouth with rubber band.
Step 5, every day is recording room temp. and humidity sooner or later, and controlling its temperature is 26 ~ 30 DEG C, and relative moisture is 60 ~ 70%, and changes Chinese cabbage leaf in time.
Step 6, every two days statistics once dead number, number of times of casting off a skin, number of cocooing.The larva cocoond on the same day being placed into bottom is covered with in the crisper of 3-5mm wet soil, hides the sealing of sturdy pines band with black cloth.
Step 7, collects with the UP pipe of 3-5ml after turning into adult, before being placed into closed window during collection, and single only collection.
Step 8, after adult enters UP pipe, pins the mouth of pipe with thumb rapidly, then puts into rapidly the beaker of 1000ml.
Step 9, beaker bottom spreads the wet soil of about 1-2mm, and the hardboard distance rim of a cup entering to be wrapped with hospital gauze (individual layer) at beaker oblique cutting is about 1.5cm (be used for collecting and steal poison moth ovum).
Step 10, puts into the sterilized penicillin bottle of (or hydromel of 3%) that 10% syrup is housed at the another side of beaker, puts into absorbent cotton or filter paper exceeds bottle 2-3cm in this penicillin bottle.
Step 11, beaker mouth black cloth hides the sealing of sturdy pines band.
Step 12, after Adult worms producting eggs, changes gauze in time.
Step 13, hatches the crisper that the wet soil being covered with 1-2mm put into by the gauze with ovum.Second generation larva after hatching treats that next time, experiment used.
The long variation diagram of body of the robber poison moth that the present embodiment is bred as shown in Figure 5; Steal the body weight change of poison moth as shown in Figure 6; As can be seen from Figure along with Chinese cabbage leaf is that diet steals poison moth, its body length and body weight increase gradually along with feeding time is passed, and rate of rise was accelerated from the 9th day.
Claims (8)
1. jujube tree steals the artificial breeding method of poison moth, it is characterized in that, specifically implements according to the following steps:
Step 1: robber poison moth ovum is placed on raise in box and hatches;
Step 2: after robber poison moth egg hatching becomes larva, put into feed in raising box, and by the gobo shutoff of its top; Every day, sooner or later temp. and humidity in box raised in record, and the replacing speed of feed is every day 3 ~ 5 times, raises;
Step 3: every two days statistics dead larvae number, number of times of casting off a skin, a number of cocooing, and be covered with in the raising box of 3 ~ 5mm wet soil bottom the larva cocoond on the same day is placed into, the gobo shutoff of its top, until steal poison moth emerging adult, to obtain final product.
2. jujube tree according to claim 1 steals the artificial breeding method of poison moth, it is characterized in that, in step 1, the bottom of raising box is equipped with the wet soil that thickness is 2 ~ 5mm.
3. jujube tree according to claim 3 steals the artificial breeding method of poison moth, and it is characterized in that, described wet soil is through ultra violet lamp 30 ~ 50min gained.
4. jujube tree according to claim 1 steals the artificial breeding method of poison moth, it is characterized in that, in step 2, feed is the one in silk flosssilk wadding wood leaf, jujube leaf or Chinese cabbage leaf.
5. jujube tree according to claim 4 steals the artificial breeding method of poison moth, it is characterized in that, in step 2, raising temperature in box is 26 ~ 30 DEG C, and relative moisture is 60 ~ 70%.
6. jujube tree according to claim 1 steals the artificial breeding method of poison moth, it is characterized in that, gained adult is placed on bottom and is equipped with captive breeding in the open-top receptacle of 1 ~ 2mm wet soil; Be placed with in open-top receptacle and steal poison moth ovum collecting rod and nutritional supplementation liquid.
7. jujube tree according to claim 6 steals the artificial breeding method of poison moth, it is characterized in that, the hardboard that described robber poison moth ovum collecting rod is monolayer medical gauze parcel or branch bar.
8. jujube tree according to claim 6 steals the artificial breeding method of poison moth, it is characterized in that, described nutritional supplementation liquid to be concentration be 10% syrup or 3% hydromel.
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| CN201510573938.7A CN105145495B (en) | 2015-09-10 | 2015-09-10 | Jujube tree steals the artificial breeding method of poison moth |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550489A (en) * | 2012-01-04 | 2012-07-11 | 河北大学 | Artificial propagation method for Ooencyrtus kuvanae |
| CN102885008A (en) * | 2012-10-31 | 2013-01-23 | 河北大学 | Artificial propagation method of Coccygomimus disparis |
| CN103960201A (en) * | 2014-05-12 | 2014-08-06 | 山西省农业科学院农产品质量安全与检测研究所 | Plutella xylostella indoor reproduction method |
| CN104186430A (en) * | 2014-08-14 | 2014-12-10 | 中国热带农业科学院椰子研究所 | Method for feeding a large number of laboratory test populations of Opisina arenosella Walkers |
| CN104757333A (en) * | 2015-04-23 | 2015-07-08 | 中国农业科学院植物保护研究所 | Artificial feed for asparagus caterpillar, preparation of artificial feed and feeding method |
| CN105379683A (en) * | 2015-11-13 | 2016-03-09 | 云南农业大学 | Artificial breeding method of gelechiid moths |
-
2015
- 2015-09-10 CN CN201510573938.7A patent/CN105145495B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550489A (en) * | 2012-01-04 | 2012-07-11 | 河北大学 | Artificial propagation method for Ooencyrtus kuvanae |
| CN102885008A (en) * | 2012-10-31 | 2013-01-23 | 河北大学 | Artificial propagation method of Coccygomimus disparis |
| CN103960201A (en) * | 2014-05-12 | 2014-08-06 | 山西省农业科学院农产品质量安全与检测研究所 | Plutella xylostella indoor reproduction method |
| CN104186430A (en) * | 2014-08-14 | 2014-12-10 | 中国热带农业科学院椰子研究所 | Method for feeding a large number of laboratory test populations of Opisina arenosella Walkers |
| CN104757333A (en) * | 2015-04-23 | 2015-07-08 | 中国农业科学院植物保护研究所 | Artificial feed for asparagus caterpillar, preparation of artificial feed and feeding method |
| CN105379683A (en) * | 2015-11-13 | 2016-03-09 | 云南农业大学 | Artificial breeding method of gelechiid moths |
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| CN105145495B (en) | 2017-10-27 |
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