CN102550489A - Artificial propagation method for Ooencyrtus kuvanae - Google Patents
Artificial propagation method for Ooencyrtus kuvanae Download PDFInfo
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- CN102550489A CN102550489A CN2012100006795A CN201210000679A CN102550489A CN 102550489 A CN102550489 A CN 102550489A CN 2012100006795 A CN2012100006795 A CN 2012100006795A CN 201210000679 A CN201210000679 A CN 201210000679A CN 102550489 A CN102550489 A CN 102550489A
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Abstract
The invention discloses an artificial propagation method for Ooencyrtus kuvanae. A caesarean egg of a tussah is used as a substitute host, the treated caesarean egg is used for inoculating Ooencyrtus kuvanae, and by culturing at normal temperature, a descendant adult fly with properties the same as that of the fly cultured from an original host egg can be obtained. By the method, Ooencyrtus kuvanae can be propagated indoors on large scale, and a practical technical means for biological control of gypsymoth in forests is provided.
Description
Technical field
The present invention relates to insect breeding field, relate in particular to the artificial fecundation method of a kind of big moth ovum encyrtid.
Background technology
Big moth ovum encyrtid (Ooencyrtus kuwanai Howard; Hymenoptera: the Encytidae) ovum of main parasitic gypsymoth (Lymantria dispar Linnaeus) also is the parasitic wasp of pine caterpillars (Dendrolimus spp.) and leucoma candida lepidoptera pest ovum such as (Stilpnotia salicis Linnaeus).Domestic known distribution in Jilin, province such as Liaoning, Beijing, Hunan, Sichuan, be distributed in Japan abroad, once introduce many state control gypsymoths on ground such as West Europe, north African and North America.
Gypsymoth all is 1 year generation all over the world, April in ovum phase from August to next year.Big moth ovum encyrtid all can be parasitic to any developmental stage of gypsymoth ovum, and can successfully accomplish the history of life.Development duration from the ovum to the adult eclosion is 21 days under 25 ℃ of conditions, is 14 days in the time of 30 ℃.At Connecticut, USA, this honeybee can accomplish for 4～5 generations every year.In the warmer southern areas of weather, can accomplish 6～7 generations in 1 year.Generally speaking, 1 the big moth ovum encyrtid that only sprouts wings in gypsymoth ovum.
Big moth ovum encyrtid is the main natural enemy of gypsymoth ovum, but not high at some local parasitic rate, the parasitic rate in the Beijing area is about 2%.The indoor gypsymoth ovum that once adopted is bred big moth ovum encyrtid.But the gypsymoth ovum both had been not easy to obtain, and operate and certain harmfulness is arranged, so this method is difficult to the batch breeding of big moth ovum encyrtid.
Tussah (Antheraea pernyi Guerin-Meneville) ovum can be used for breeding egg parasitoidses such as white instep anastatus japonicus (Pseudanstatus albitarsis Ashmead) and multiple oophagous trichogrammae.Egg of oak silkworm has stronger parasitic potential than other alternative host egg to parasitic wasp as substituting the host, and the advantage aspect preservation and rejuvenation.Correlation techniques such as the acquisition of egg of oak silkworm, preservation, inoculation are comparative maturity also.Do not find that in the open air big moth ovum encyrtid can parasitic egg of oak silkworm, up to the present, also not seeing has the report that utilizes egg of oak silkworm to breed big moth ovum encyrtid.
Summary of the invention
Technical problem to be solved by this invention provides the artificial fecundation method of a kind of big moth ovum encyrtid, uses this method the technological means of practicality to be provided for woodland biological control gypsymoth the big moth ovum of indoor large-scale breeding encyrtid.
In order to solve the problems of the technologies described above, the present invention takes following technical scheme:
The artificial fecundation method of a kind of big moth ovum encyrtid is characterized in that comprising the steps:
(a) after being sprouted wings, tussah chrysalis gets the ovum of cutting open the belly ,-4～-2 ℃ of following freezings 15～60 days;
(b) ovum of cutting open the belly behind the freezing was thawed under 5～12 ℃ 1～2 day;
(c) the inoculation box of putting into big moth ovum encyrtid adult of the ovum of cutting open the belly after will thawing is inoculated; The number ratio of ovum and big moth ovum encyrtid adult of cutting open the belly is 3～5: 1; And be that 15% hydromel is fed big moth ovum encyrtid adult with mass concentration, inoculation time is 3～5 days;
(d) the inoculation back that finishes is taken out the ovum of cutting open the belly and is cultivated to sprout wings down at 22～26 ℃ and big moth ovum encyrtid.
Technique scheme, can do following optimization:
(a) after being sprouted wings, tussah chrysalis gets the ovum of cutting open the belly ,-3 ℃ of following freezings 40 days;
(b) ovum of cutting open the belly behind the freezing was thawed under 10 ℃ 1.5 days;
(c) the inoculation box of putting into big moth ovum encyrtid adult of the ovum of cutting open the belly after will thawing is inoculated; The number ratio of ovum and big moth ovum encyrtid adult of cutting open the belly is 4: 1; And be that 15% hydromel is fed big moth ovum encyrtid adult with mass concentration, inoculation time is 4 days;
(d) the inoculation back that finishes is taken out the ovum of cutting open the belly and is cultivated to sprout wings down at 25 ℃ and big moth ovum encyrtid.
The invention has the advantages that: the normal ovum that is produced after the female worm of tussah sprouts wings can not be as the host of big moth ovum encyrtid; The ovum of cutting open the belly after the present invention sprouts wings to the female worm of tussah is handled; Make the ovum of cutting open the belly after the processing can be used as the alternative host of big moth ovum encyrtid, thereby accomplished the mass artificial propagation of big moth ovum encyrtid.Statistics shows; The female individuals of the big moth ovum encyrtid of above-mentioned artificial propagation is bigger than the female individuals of sprouting wings in the gypsymoth ovum; In vigorous health, the male difference in size is not obvious, in addition; The big moth ovum encyrtid that the big moth ovum encyrtid of artificial propagation and gypsymoth ovum are sprouted wings is all not remarkable in aspect difference such as life-span, male and female sex ratios, explains that the filial generation of this artificial fecundation method breeding is very similar with the filial generation of big moth ovum encyrtid natural propagation.
Embodiment
Embodiment one: substitute the acquisition of host egg
Utilize egg of oak silkworm to breed big moth ovum encyrtid as substituting the host.Buy female tussah chrysalis on the market.Pupa is before use in 0～5 ℃ of preservation.During use tussah chrysalis is put under 25 ℃ of room temperature conditions, it is sprouted wings for becoming moth.Then female one-tenth moth is dissected, obtained to dissect ovum, ovum is cleaned, dried, reject abnormal ovum and obtain normal ovum.
Embodiment two:
(a) after being sprouted wings, tussah chrysalis gets the ovum of cutting open the belly ,-4 ℃ of following freezings 15 days;
(b) with the transition 2 days of thawing in the climatic cabinate of ovum under 5 ℃ of cutting open the belly behind the freezing;
(c) the inoculation box of putting into big moth ovum encyrtid adult of the ovum of cutting open the belly after will thawing is inoculated; The number ratio of ovum and big moth ovum encyrtid adult of cutting open the belly is 3: 1 (300 ovum of 100 big moth ovum encyrtids inoculations); And be that 15% hydromel is fed big moth ovum encyrtid adult with mass concentration, inoculation time is 5 days;
(d) inoculate the back taking-up that finishes and cut open the belly ovum 22 ℃ of cultivations down, sprouting wings about 19 days big moth ovum encyrtid, maximum 8 the big moth ovum encyrtids that sprout wings of ovum, 2 big moth ovum encyrtids of minimum emergence, 5.2 big moth ovum encyrtids of average every ovum emergence.
Embodiment three:
(a) after being sprouted wings, tussah chrysalis gets the ovum of cutting open the belly ,-2 ℃ of following freezings 60 days;
(b) with the transition 1 day of thawing in the climatic cabinate of ovum under 12 ℃ of cutting open the belly behind the freezing;
(c) the inoculation box of putting into big moth ovum encyrtid adult of the ovum of cutting open the belly after will thawing is inoculated; The number ratio of ovum and big moth ovum encyrtid adult of cutting open the belly is 5: 1 (500 ovum of 100 big moth ovum encyrtids inoculations); And be that 15% hydromel is fed big moth ovum encyrtid adult with mass concentration, inoculation time is 3 days;
(d) inoculate the back taking-up that finishes and cut open the belly ovum 26 ℃ of cultivations down, sprouting wings about 17 days big moth ovum encyrtid, maximum 9 the big moth ovum encyrtids that sprout wings of ovum, 3 big moth ovum encyrtids of minimum emergence, 5.5 big moth ovum encyrtids of average every ovum emergence.
Embodiment four:
(a) after being sprouted wings, tussah chrysalis gets the ovum of cutting open the belly ,-3 ℃ of following freezings 40 days;
(b) with the transition 1.5 days of thawing in the climatic cabinate of ovum under 10 ℃ of cutting open the belly behind the freezing;
(c) the inoculation box of putting into big moth ovum encyrtid adult of the ovum of cutting open the belly after will thawing is inoculated; The number ratio of ovum and big moth ovum encyrtid adult of cutting open the belly is 4: 1 (400 ovum of 100 big moth ovum encyrtids inoculations); And be that 15% hydromel is fed big moth ovum encyrtid adult with mass concentration, inoculation time is 4 days;
(d) inoculate the back taking-up that finishes and cut open the belly ovum 25 ℃ of cultivations down, sprouting wings about 18 days big moth ovum encyrtid, maximum 11 the big moth ovum encyrtids that sprout wings of ovum, 3 big moth ovum encyrtids of minimum emergence, 6 big moth ovum encyrtids of average every ovum emergence.
Embodiment five:
The time of egg of oak silkworm preservation under-4～-2 ℃ of conditions is determining the success rate of big moth ovum encyrtid breeding.0～7 day (not containing 7 days) of preservation, it is perishable mouldy that egg of oak silkworm takes out the back; 7～15 days (not containing 15 days) of preservation can breed big moth ovum encyrtid, but big moth ovum encyrtid breeding success rate is low, is 5%; Preservation 15～60 days, the one-tenth rate of big moth ovum encyrtid breeding reaches 80%, and effect is best.
Embodiment six:
Individual size: each 30 of the big moth ovum of male and female encyrtids that hatch in each 30 of the big moth ovum of male and female encyrtids that egg of oak silkworm is hatched and the gypsymoth ovum are measured size respectively, obtain data.Statistics shows, the female big moth ovum encyrtid individuality that the mean value of the big moth ovum encyrtid female individuals that of from egg of oak silkworm, sprouting wings than sprouting wings in the gypsymoth ovum is bigger, significant difference; And the mean value of male size is than gypsymoth ovum the individual less than normal of the big moth ovum encyrtid that sprout wings, but difference is not remarkable.
Life-span: the big moth ovum encyrtid that the gypsymoth ovum is sprouted wings, drone life-span average out to 15.95 days, queen bee life-span average out to 20.94 days; The big moth ovum encyrtid that egg of oak silkworm is sprouted wings, drone life-span average out to 10.38 days, queen bee life-span average out to 15.01 days.Can satisfy needs as the biological and ecological methods to prevent plant disease, pests, and erosion factor.
Male and female sex ratio: respectively the sprout wings male and female sex of 1000 big moth ovum encyrtid of gypsymoth ovum and egg of oak silkworm is tested, show that egg of oak silkworm the sprout wings female and male ratio of the big moth ovum encyrtid that of the big moth ovum encyrtid that and gypsymoth ovum of sprouting wings does not have significant difference.
Claims (2)
1. the artificial fecundation method of one kind big moth ovum encyrtid is characterized in that comprising the steps:
(a) after being sprouted wings, tussah chrysalis gets the ovum of cutting open the belly ,-4～-2 ℃ of following freezings 15～60 days;
(b) ovum of cutting open the belly behind the freezing was thawed under 5～12 ℃ 1～2 day;
(c) the inoculation box of putting into big moth ovum encyrtid adult of the ovum of cutting open the belly after will thawing is inoculated; The number ratio of ovum and big moth ovum encyrtid adult of cutting open the belly is 3～5: 1; And be that 15% hydromel is fed big moth ovum encyrtid adult with mass concentration, inoculation time is 3～5 days;
(d) the inoculation back that finishes is taken out the ovum of cutting open the belly and is cultivated to sprout wings down at 22～26 ℃ and big moth ovum encyrtid.
2. the artificial fecundation method of big moth ovum encyrtid as claimed in claim 1 is characterized in that:
(a) after being sprouted wings, tussah chrysalis gets the ovum of cutting open the belly ,-3 ℃ of following freezings 40 days;
(b) ovum of cutting open the belly behind the freezing was thawed under 10 ℃ 1.5 days;
(c) the inoculation box of putting into big moth ovum encyrtid adult of the ovum of cutting open the belly after will thawing is inoculated; The number ratio of ovum and big moth ovum encyrtid adult of cutting open the belly is 4: 1; And be that 15% hydromel is fed big moth ovum encyrtid adult with mass concentration, inoculation time is 4 days;
(d) the inoculation back that finishes is taken out the ovum of cutting open the belly and is cultivated to sprout wings down at 25 ℃ and big moth ovum encyrtid.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103918613A (en) * | 2014-04-23 | 2014-07-16 | 安徽师范大学 | Pupal-stage parasitic wasp artificial-propagation method, wasp-keeping box and manufacturing method of wasp-keeping box |
| CN105145495A (en) * | 2015-09-10 | 2015-12-16 | 延安大学 | Artificial breeding method for jujube tree porthesia similis |
| CN107455343A (en) * | 2017-09-09 | 2017-12-12 | 中国农业科学院植物保护研究所 | Predatory Mites are cut open the belly the acquisition methods of ovum |
| KR20190027227A (en) * | 2017-09-06 | 2019-03-14 | 순천대학교 산학협력단 | A method for artificial rearing Oorencyrtus nezarae |
| KR20190027226A (en) * | 2017-09-06 | 2019-03-14 | 순천대학교 산학협력단 | Artificial egg bag for rearing Oorencyrtus nezarae |
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| RU2728337C1 (en) * | 2019-10-08 | 2020-07-29 | Федеральное бюджетное учреждение "Всероссийский научно-исследовательский институт лесоводства и механизации лесного хозяйства (ФБУ ВНИИЛМ) | Method for cultivation of laboratory culture of entomophage egg parasite ooencyrtus kuvanae (howard) |
| RU2730035C1 (en) * | 2019-10-08 | 2020-08-14 | Федеральное бюджетное учреждение "Всероссийский научно-исследовательский институт лесоводства и механизации лесного хозяйства (ФБУ ВНИИЛМ) | Method for mass rearing of egg parasite ooencyrtus kuvanae (howard) |
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Cited By (9)
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| CN103918613A (en) * | 2014-04-23 | 2014-07-16 | 安徽师范大学 | Pupal-stage parasitic wasp artificial-propagation method, wasp-keeping box and manufacturing method of wasp-keeping box |
| CN103918613B (en) * | 2014-04-23 | 2016-04-27 | 安徽师范大学 | A method for parasitic wasp artificial propagation in pupa time, and raise bees box and preparation method |
| CN105145495A (en) * | 2015-09-10 | 2015-12-16 | 延安大学 | Artificial breeding method for jujube tree porthesia similis |
| CN105145495B (en) * | 2015-09-10 | 2017-10-27 | 延安大学 | Jujube tree steals the artificial breeding method of poison moth |
| KR20190027227A (en) * | 2017-09-06 | 2019-03-14 | 순천대학교 산학협력단 | A method for artificial rearing Oorencyrtus nezarae |
| KR20190027226A (en) * | 2017-09-06 | 2019-03-14 | 순천대학교 산학협력단 | Artificial egg bag for rearing Oorencyrtus nezarae |
| KR102032946B1 (en) | 2017-09-06 | 2019-10-16 | 순천대학교 산학협력단 | Artificial egg bag for rearing Oorencyrtus nezarae |
| KR102086772B1 (en) | 2017-09-06 | 2020-03-09 | 순천대학교 산학협력단 | A method for artificial rearing Oorencyrtus nezarae |
| CN107455343A (en) * | 2017-09-09 | 2017-12-12 | 中国农业科学院植物保护研究所 | Predatory Mites are cut open the belly the acquisition methods of ovum |
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