CN1948457A - Method of increasing wild cordyceps yield - Google Patents
Method of increasing wild cordyceps yield Download PDFInfo
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- CN1948457A CN1948457A CN 200610123384 CN200610123384A CN1948457A CN 1948457 A CN1948457 A CN 1948457A CN 200610123384 CN200610123384 CN 200610123384 CN 200610123384 A CN200610123384 A CN 200610123384A CN 1948457 A CN1948457 A CN 1948457A
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- 238000000034 method Methods 0.000 title claims abstract description 25
- 241000190633 Cordyceps Species 0.000 title claims description 12
- 241000330899 Hepialus Species 0.000 claims abstract description 104
- 239000002689 soil Substances 0.000 claims abstract description 34
- 241001248610 Ophiocordyceps sinensis Species 0.000 claims description 77
- 239000003085 diluting agent Substances 0.000 claims description 23
- 241000233866 Fungi Species 0.000 claims description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 208000015181 infectious disease Diseases 0.000 claims description 8
- 241000802118 Thitarodes gonggaensis Species 0.000 claims description 6
- 238000005507 spraying Methods 0.000 claims description 6
- 241000330914 Thitarodes oblifurcus Species 0.000 claims description 4
- 244000005700 microbiome Species 0.000 claims description 4
- 244000304222 Melaleuca cajuputi Species 0.000 claims description 3
- 235000001167 Melaleuca cajuputi Nutrition 0.000 claims description 3
- 240000001307 Myosotis scorpioides Species 0.000 claims description 2
- 150000001875 compounds Chemical class 0.000 claims description 2
- 238000011081 inoculation Methods 0.000 claims description 2
- 206010020880 Hypertrophy Diseases 0.000 abstract description 13
- 238000009395 breeding Methods 0.000 abstract description 11
- 230000001488 breeding effect Effects 0.000 abstract description 11
- 206010011732 Cyst Diseases 0.000 abstract 2
- 208000031513 cyst Diseases 0.000 abstract 2
- 238000010790 dilution Methods 0.000 abstract 2
- 239000012895 dilution Substances 0.000 abstract 2
- 210000004681 ovum Anatomy 0.000 description 15
- 230000004720 fertilization Effects 0.000 description 11
- 102000002322 Egg Proteins Human genes 0.000 description 9
- 108010000912 Egg Proteins Proteins 0.000 description 9
- 241000238631 Hexapoda Species 0.000 description 8
- 235000013601 eggs Nutrition 0.000 description 6
- 239000007921 spray Substances 0.000 description 5
- 241000382353 Pupa Species 0.000 description 4
- 241000235349 Ascomycota Species 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 241001480006 Clavicipitaceae Species 0.000 description 2
- 241000221775 Hypocreales Species 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 244000283482 Alloteropsis cimicina Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241000288673 Chiroptera Species 0.000 description 1
- 235000017898 Digitaria ciliaris Nutrition 0.000 description 1
- 241000130660 Hepialidae Species 0.000 description 1
- 241000330919 Hepialus renzhiensis Species 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 241000257211 Sordariomycetidae Species 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 230000032696 parturition Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
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Abstract
This invention relates to a method of increasing the production of Qinghai-Tibet plateau wild aweto, including putting Hepialus zygote or/and Hepialus larva at Hepialus Suitable habitat: when Hepialus larva grows at 6-8 instar, thecaspore which is ejected by hiascent of aweto ascocarp cupular part is infected to form worm breeding daughter cyst, the aweto naturally grows at Hepialus Suitable habitat of Qinghai-Tibet plateau; Or meanwhile collecting aweto thecaspore to prepare thecaspore dilution or/and aweto hyphostroma and conidiospore are prepared to be hyphostroma and conidiospore dilution, carrying out conspergere to infect, so that it can efficiently infect Hepialus larva to forming worm breeding daughter cyst eumycete; In May of the next year, after Qinghai-Tibet plateau taele is melted, when aweto stroma outerop soil surface, and before stroma head ascocarp has not grow hypertrophy, reaping aweto. according to this method, it can reap 14.4-165 ten thousand aweto by putting 40-370 ten thousand Hepialus zygote or/and Hepialus larva at Hepialus Suitable habitat per hectare.
Description
Technical field
The present invention relates to a kind of method that improves Qinghai-Tibet wild cordyceps yield.
Background technology
Cordyceps sinensis [Cordyceps sinensis (Berkeley) Saccardo] is that lepidopteran Hepialidae bat belongs to a kind of entomogenous fungi that (Hepialus) larva is formed by the parasitic back of China pilose spore (Hirsutella sinensis X.J.liu et al.), the Ascomycota (Ascomycota) that belongs to mycota (Fungi), Ascomycetes (Ascomycetcs), excrement shell bacterium subclass (Sordariomycetidae), Hypocreales (Hypocreales), Clavicipitaceae (Clavicipitaceae), Chinese caterpillar fungus Pseudomonas [Cordyceps (Frey) Link.] a kind of, its formal name used at school is Cordyceps sinensis (Berkeley) Saccardo.Discover that the Cordyceps sinensis perfect stage that is distributed in Qinghai-Tibet producing regions such as Tibet, Qinghai, Sichuan, Gansu, Yunnan all belongs to same species [Cordyceps sinensis (Berkeley) Saccardo].The Cordyceps sinensis stroma was grown and is exposed native face after May, Qinghai-Tibet Platean frozen soil melted then, June, the stroma head was grown hypertrophy rapidly, form ascoma and thecaspore, sophisticated thecaspore ejects from the ascoma open top, with infecting the Hepialus larva that moves in the soil in snow-broth and the rainwater infiltration soil, in May next year with the temperature rises, the Cordyceps sinensis stroma is broken through the soil.Along with the increase of people to the Cordyceps sinensis demand, Cordyceps sinensis is excavated the potential eco-catastrophe of being brought by transition, causes the Cordyceps sinensis resource to face the threat of petering out.
The Hepialus insect of hitherto reported surpasses 60 kinds, and wherein Yunnan is 20 kinds, 17 kinds in Tibet, 12 kinds in Sichuan, 9 kinds in Qinghai, 9 kinds in Gansu.Hepialus larva is an omnivorous insect, and battalion's tunnel like troglodytism is a food with tender of plant in soil.Though different types of bat has certain difference on biology and ecology, be characterized in needing to experience veryer long larval stage, and have tangible digenesis phenomenon.Bat has typical Characteristics of Vertical Distribution, and its distribution lower limit is at the 3000m of latitude regional height above sea level by north, and at the about 2500m of latitude regional height above sea level by north; Be limited to height above sea level 5100m in the distribution; The growth height above sea level that adapts to most is 3600～5000m; Optimum soil for growth is high and cold grassy marshland and high-cold scrub soil.Because bat is finished a life cycle and need experience ovum, larva, pupa, adult four-stage, annual April October to next year frozen soil phase Hepialus larva in soil, be in dormant state, the growth and development stage that Hepialus larva need experience for 6～8 length of times approximately just can be pupated, concrete fate is different because of kind, need 996d approximately as the cajaput Hepialus larva, the about 1000d of Hepialus kangdingensis Chu et Wang, Hepialus gonggaensis 875～1040d, and all can in soil, find the difference Hepialus larva in the length of times all the year round.Annual late May part Hepialus larva formation pupa turns into adult 6～August.Adult is many o'clock to sprout wings in every day 17～20, near the female moth of post-coitum looses ovum while fluttering immediately and originates in several square metres the grassy marshland thick grass or in the shrubbery vegetation, and about 500 of general every female moth eggs.The comprehensive restriction control of breeding multiple ecological factors such as being subjected to ecogeography distribution, food, vegetation, Soil structure, temperature, humidity and natural enemy of Hepialus insect in the physical environment, the breed speed of Hepialus larva in physical environment has become the key factor that restricts yield of Qinghai Tibet plateau cordyceps.
Summary of the invention
The purpose of this invention is to provide a kind of method that improves yield of Qinghai Tibet plateau cordyceps.
The present invention is by throwing in hepialus larva insect species breeding hepialus larva to improve wild cordyceps yield at Qinghai-Tibet Hepialus insect suitable habitat.
Method of the present invention is that fertilization bat worm's ovum or/and the Hepialus larva of fertilization bat egg hatch is invested in Qinghai-Tibet bat suitable habitat as hepialus larva insect species, is effectively promoted Qinghai-Tibet bat suitable habitat Hepialus larva quantity and breeds density; When the Hepialus larva in the bat suitable habitat is grown to 6～8 length of times, the thecaspore that launches through the Cordyceps sinensis ascoma open top of bat suitable habitat self-sow infects and forms entomogenous fungi, in May next year Qinghai-Tibet Platean frozen soil melt the back and grow at the Cordyceps sinensis stroma and expose native face and the stroma head ascoma Cordyceps sinensis of in time gathering before not growing hypertrophy yet.
Method according to the invention described above, when the Hepialus larva in the bat suitable habitat of throwing in hepialus larva insect species is grown to 6～8 length of times, can also adopt artificial challenge's method simultaneously again, comprise: collect the Cordyceps sinensis thecaspore and be mixed with the thecaspore diluent or/and Cordyceps mycelium and conidium are mixed with mycelium and conidium diluent, carrying out the artificial spraying then infects, to improve infection rate, effectively infect the Hepialus larva that moves in the soil and form entomogenous fungi; Thereby further improve the output of Cordyceps sinensis.
Method according to the invention described above; usually throw in Hepialus larva 40～3,700,000 of 40～3,700,000 in bat zygote or bat incubating oosperm at per hectare bat suitable habitat, perhaps the per hectare bat suitable habitat larva totally 40～3,700,000 (head) of throwing in bat zygote and bat incubating oosperm.Per hectare throw in bat zygote or/and the bat suitable habitat of Hepialus larva can be gathered Cordyceps sinensis 14.4～1,650,000 piece.
The fertilization bat worm's ovum that is invested in Qinghai-Tibet bat suitable habitat of the present invention can be gathered in the crops its zygote of producing through post-coitum by catching the bat adult from other bat suitable habitat; Perhaps cultivate Hepialus larva to adult, from this hepialus genus captive field, catch the bat adult again and gather in the crops its zygote of producing through post-coitum by set up the warmhouse booth hepialus genus captive field at the bat suitable habitat.The described Hepialus larva that is invested in the fertilization bat egg hatch of Qinghai-Tibet bat suitable habitat can directly be obtained by the method for resulting fertilization bat worm's ovum by common artificial incubation.
The collection worm summer grass seed method ascosporous that the present invention relates to is normally: the fresh Cordyceps sinensis that will launch not yet from the thecaspore that gather the Qinghai-Tibet Platean inserts in the glassware that fills sterilized water, make Cordyceps sinensis stroma ascoma exceed the water surface (add usually in the glassware amount of sterilized water be about be dipped to the Cordyceps sinensis length about 1/3rd that inserts) up, and place glassware under the sunlight or 30W～60W UV-lamp under shine 8～24h after, the Cordyceps sinensis thecaspore launches in sterilized water, collects the sophisticated Cordyceps sinensis thecaspore that launches in sterilized water through separating (can adopt the isolating method of common whizzer); It is standby thecaspore can be placed 4 ℃ of refrigerators to preserve; The Cordyceps sinensis thecaspore diluent concentration of preparation is generally: water and thecaspore part by weight 1000000: 0.5～1.Can adopt spraying method during use, every 10M
2The area usage quantity is 20～100g thecaspore diluent.
The compound method of Cordyceps mycelium that the present invention relates to and conidium diluent is normally: fresh Cordyceps sinensis thecaspore or the fresh winter worm summer herb thallus that will gather from the Qinghai-Tibet Platean, according to common industrial microorganism method separate, purifying, inoculation and cultivate and be mycelium and conidium; The mycelium and the conidium diluent concentration of preparation are generally: water and mycelium and conidium part by weight are 1000000: 2～3.Can adopt spraying method during use, every 10M
2The area usage quantity is 20～100g mycelium and conidium diluent.
The Hepialus insect suitable habitat that the present invention relates to comprises Qinghai-Tibet high and cold grassy marshland herbaceous plant vegetation region and/or the scrub plant district of height above sea level from 2900 meters to 5000m.
The Hepialus insect that the present invention relates to (being called for short bat) comprising: the Chinese caterpillar fungus bat, Hepialus minyuancus (Hepialus minyuancus), the cajaput bat, Hepialus lagii Yan (Hepialus lagii Yan), Hepialus gonggaensis (Hepialus gonggaensis S.Q.Fu ﹠amp; T.F.Fuang), Hepialus guidera Yan (Hepialus guidera Yan), Hepialus oblifurcus Chu et Wang (Hepialus oblifurcus Chu et Wang), Baima bat, the people props up bat (Hepialusrenz hiensis), Deqie bat, Altay bat, such as bat (Hepialus bi ruensisHepial Fu a Huang) and scholar bat (Hepialus xuezheiensis sp.nov.), can be wherein one or more.
Embodiment
Below in conjunction with embodiment the present invention is described in further detail.
Embodiment one:
Throw in 1,250,000 of Hepialus lagii Yan worm's ovums as hepialus larva insect species breeding hepialus larva in Qinghai-Tibet bat suitable habitat per hectare; When the Hepialus larva in the bat suitable habitat is grown to 6～8 length of times, launch thecaspore through the Cordyceps sinensis ascoma open top of bat suitable habitat self-sow and infect the Hepialus larva move in the soil and form entomogenous fungi, in May next year Qinghai-Tibet Platean frozen soil melt the back and grow at the Cordyceps sinensis stroma and expose native face and before the stroma head ascoma is not grown hypertrophy yet, in time gather Cordyceps sinensis.By statistics, the bat suitable habitat of per hectare input bat worm's ovum can be gathered in the crops about 420,000 of Cordyceps sinensis.
Embodiment two:
Qinghai-Tibet alpine condition Hepialus insect suitable habitat per hectare throw in Hepialus minyuancus and Hepialus gonggaensis zygote totally 370 ten thousand as worm kind breeding hepialus larva; When the Hepialus larva in the bat suitable habitat was grown to 6～8 length of times, the thecaspore with the fresh Cordyceps sinensis of collecting of gathering from the Qinghai-Tibet Platean launches was mixed with the thecaspore diluent by water and thecaspore part by weight, every 10M at 1000000: 1
2Area sprays infection with 100g thecaspore diluent, can effectively infect the Hepialus larva that moves in the soil and form entomogenous fungi; After May next year, Qinghai-Tibet Platean frozen soil melted, expose native face and before the stroma head ascoma is not grown hypertrophy yet, in time gather Cordyceps sinensis in the growth of Cordyceps sinensis stroma.By statistics, the bat suitable habitat of per hectare input bat adult can be gathered in the crops about 1,650,000 of Cordyceps sinensis.
Embodiment three:
Drop into 3,000,000 in Hepialus guidera Yan zygote as worm kind breeding hepialus larva in Qinghai-Tibet alpine condition Hepialus insect suitable habitat per hectare; When the Hepialus larva in the bat suitable habitat is grown to 6～8 length of times, with the fresh Cordyceps sinensis thecaspore of collecting, be mixed with the thecaspore diluent at 1000000: 0.5 by water and thecaspore part by weight, and fresh Cordyceps sinensis further cultivated through industrial microorganism separation and Culture technology usually be mycelium and conidium, be mixed with mycelium and conidium diluent at 1000000: 2 by water and mycelium and conidium part by weight; Every 10M
2Area uses thecaspore diluent and mycelium and each 20g of conidium diluent to spray infection, can effectively infect the Hepialus larva that moves in the soil and form entomogenous fungi; After May next year, Qinghai-Tibet Platean frozen soil melted, expose native face and before the stroma head ascoma is not grown hypertrophy yet, in time gather Cordyceps sinensis in the growth of Cordyceps sinensis stroma.By statistics, the bat suitable habitat of per hectare input bat adult can be gathered in the crops about 121.5 ten thousand of Cordyceps sinensis.
Embodiment four:
Throw in 2,450,000 of the larvas of Hepialus minyuancus fertilization egg hatch in Qinghai-Tibet alpine condition Hepialus insect suitable habitat per hectare; When the Hepialus larva in the bat suitable habitat is grown to 6～8 length of times, open the door through the Cordyceps sinensis ascoma top of bat suitable habitat self-sow and to launch thecaspore and infect the Hepialus larva that moves in the soil and form entomogenous fungi, in May next year Qinghai-Tibet Platean frozen soil melt the back and grow at the Cordyceps sinensis stroma and expose native face and before the stroma head ascoma is not grown hypertrophy yet, in time gather Cordyceps sinensis.By statistics, the bat suitable habitat of per hectare input bat pupa can be gathered in the crops about 750,000 of Cordyceps sinensis.
Embodiment five:
Throw in 1,650,000 of Hepialus gonggaensis worm's ovums as hepialus larva insect species breeding hepialus larva in the suitable per hectare of giving birth to of Qinghai-Tibet alpine condition Hepialus insect; When the Hepialus larva in the bat suitable habitat is grown to 6～8 length of times, launch the thecaspore infection through the Cordyceps sinensis ascoma open top of bat suitable habitat self-sow and form entomogenous fungi, after May next year, Qinghai-Tibet Platean frozen soil melted, expose native face and before the stroma head ascoma is not grown hypertrophy yet, in time gather Cordyceps sinensis in the growth of Cordyceps sinensis stroma.By statistics, the bat suitable habitat of per hectare input bat worm's ovum can be gathered in the crops about 58.5 ten thousand of Cordyceps sinensis.
Embodiment six:
Throw in 2,000,000 of the larvas of Hepialus guidera Yan fertilization egg hatch in Qinghai-Tibet alpine condition Hepialus insect suitable habitat per hectare; When the Hepialus larva in the bat suitable habitat is grown to 6～8 length of times, launch thecaspore through the Cordyceps sinensis ascoma open top of bat suitable habitat self-sow and infect the Hepialus larva move in the soil and form entomogenous fungi, in May next year Qinghai-Tibet Platean frozen soil melt the back and grow at the Cordyceps sinensis stroma and expose native face and before the stroma head ascoma is not grown hypertrophy yet, in time gather Cordyceps sinensis.By statistics, the bat suitable habitat of per hectare input bat worm's ovum can be gathered in the crops about 690,000 of Cordyceps sinensis.
Embodiment seven:
Throw in 1,700,000 of Hepialus oblifurcus Chu et Wang fertilization worm's ovums as worm kind breeding hepialus larva in Qinghai-Tibet alpine condition Hepialus insect suitable habitat per hectare; When the Hepialus larva in the bat suitable habitat is grown to 6～8 length of times, adopt the fresh Cordyceps sinensis of gathering to collect the thecaspore that launches, be mixed with the thecaspore diluent at 1000000: 0.6 by water and thecaspore part by weight, every 10M from the Qinghai-Tibet Platean
2The area usage quantity is that 80g sprays infection, can effectively infect the Hepialus larva that moves in the soil and form entomogenous fungi, after May next year, Qinghai-Tibet Platean frozen soil melted, expose native face and before the stroma head ascoma is not grown hypertrophy yet, in time gather Cordyceps sinensis in the growth of Cordyceps sinensis stroma.By statistics, the bat suitable habitat of per hectare input bat adult can be gathered in the crops about 720,000 of Cordyceps sinensis.
Embodiment eight:
Throw in 1,350,000 of Hepialus lagii Yan fertilization worm's ovums in Qinghai-Tibet alpine condition Hepialus insect suitable habitat per hectare, the bat worm's ovum is hatched naturally at the bat suitable habitat and is larva, throws in simultaneously by the be fertilized larva of egg hatches of 1,250,000 scholar bats; When the Hepialus larva in the bat suitable habitat is grown to 6～8 length of times, it is mycelium and conidium that fresh Cordyceps sinensis is further cultivated through common industrial microorganism separation and Culture technology, be mixed with mycelium and conidium diluent, every 10M at 1000000: 3 in water and mycelium and conidium ratio
2The area usage quantity is that 60g sprays infection, can effectively infect the Hepialus larva that moves in the soil and form entomogenous fungi, after May next year, Qinghai-Tibet Platean frozen soil melted, expose native face and before the stroma head ascoma is not grown hypertrophy yet, in time gather Cordyceps sinensis in the growth of Cordyceps sinensis stroma.By statistics, the bat suitable habitat of per hectare input bat adult can be gathered in the crops about 540,000 of Cordyceps sinensis.
Embodiment nine:
Drop into 400,000 of Hepialus minyuancus fertilization worm's ovums as hepialus larva insect species breeding hepialus larva in Qinghai-Tibet alpine condition Hepialus insect suitable habitat per hectare; When the Hepialus larva in the bat suitable habitat is grown to 6～8 length of times, launch thecaspore through the Cordyceps sinensis ascoma open top of bat suitable habitat self-sow and infect the Hepialus larva move in the soil and form entomogenous fungi, in May next year Qinghai-Tibet Platean frozen soil melt the back and grow at the Cordyceps sinensis stroma and expose native face and before the stroma head ascoma is not grown hypertrophy yet, in time gather Cordyceps sinensis.By statistics, the bat suitable habitat of per hectare input bat pupa can be gathered in the crops about 14.4 ten thousand of Cordyceps sinensis.
Embodiment ten:
Drop into 2,500,000 of Hepialus guidera Yan fertilization worm's ovums as hepialus larva insect species breeding hepialus larva in Qinghai-Tibet alpine condition Hepialus insect suitable habitat per hectare; When the Hepialus larva in the bat suitable habitat is grown to 6～8 length of times, launching thecaspore through the Cordyceps sinensis ascoma open top of bat suitable habitat self-sow infects, and adopt the fresh Cordyceps sinensis of gathering to collect the thecaspore that launches from the Qinghai-Tibet Platean, be mixed with the thecaspore diluent at 1000000: 0.5 by water and thecaspore part by weight, every 10M
2The area usage quantity is that 30g sprays infection, make the Hepialus larva that moves in the soil form entomogenous fungi, after May next year, Qinghai-Tibet Platean frozen soil melted, expose native face and before the stroma head ascoma is not grown hypertrophy yet, in time gather Cordyceps sinensis in the growth of Cordyceps sinensis stroma.By statistics, the bat suitable habitat of per hectare input Hepialus larva can be gathered in the crops about 990,000 of Cordyceps sinensis.
Claims (6)
1. method that improves yield of Qinghai Tibet plateau cordyceps, this method comprises: throw in bat zygote or/and the Hepialus larva of bat incubating oosperm at Qinghai-Tibet bat suitable habitat, when the Hepialus larva in the bat suitable habitat is grown to 6～8 length of times, the thecaspore natural infection of launching through the Cordyceps sinensis ascoma open top of bat suitable habitat self-sow forms entomogenous fungi, exposes native face in the growth of Cordyceps sinensis stroma after May next year, Qinghai-Tibet Platean frozen soil melted, and the stroma head ascoma is not grown the loose Cordyceps sinensis of in time gathering before yet.
2. in accordance with the method for claim 1, it is characterized in that when the Hepialus larva of bat suitable habitat is grown to 6～8 length of times, collect the Cordyceps sinensis thecaspore and be mixed with the thecaspore diluent or/and Cordyceps mycelium and conidium are mixed with mycelium and conidium diluent, carry out the artificial spraying then, infect Hepialus larva and form entomogenous fungi.
3. according to claim 1 or 2 described methods; it is characterized in that describedly throwing in bat zygote or/and the quantity of the Hepialus larva of bat incubating oosperm is at Qinghai-Tibet bat suitable habitat: in Hepialus larva 40～3,700,000 that per hectare bat suitable habitat is thrown in 40～3,700,000 in bat zygote or bat incubating oosperm, perhaps per hectare bat suitable habitat larva totally 40～3,700,000 or the head of throwing in bat zygote and bat incubating oosperm.
4. according to claim 1 or 2 described methods, it is characterized in that described bat comprises: Chinese caterpillar fungus bat, Hepialus minyuancus, cajaput bat, Hepialus lagii Yan, Hepialus gonggaensis, Hepialus guidera Yan, Hepialus oblifurcus Chu et Wang, Baima bat, people prop up bat, Deqie bat, Altay bat, such as in bat and the scholar bat one or more.
5. in accordance with the method for claim 2, it is characterized in that the thecasporous method of described collection Cordyceps sinensis is: the fresh Cordyceps sinensis that will launch not yet from the thecaspore that gather the Qinghai-Tibet Platean inserts in the glassware that fills sterilized water, make Cordyceps sinensis stroma ascoma exceed the water surface up, and place glassware under the sunlight or 30W～60W UV-lamp under shine 8～24h after, separated and collected is launched the sophisticated Cordyceps sinensis thecaspore in sterilized water; The concentration of described Cordyceps sinensis thecaspore diluent is: water and thecaspore part by weight 1000000: 0.5～1; Every 10M when spraying
2The area usage quantity is 20～100g thecaspore diluent.
6. in accordance with the method for claim 2, the compound method that it is characterized in that described Cordyceps mycelium and conidium diluent is: fresh Cordyceps sinensis thecaspore or the fresh Cordyceps sinensis that will gather from the Qinghai-Tibet Platean, according to common industrial microorganism method separate, purifying, inoculation and cultivate and be mycelium and conidium, be 1000000: 2～3 to be mixed with mycelium and conidium diluent by water and mycelium and conidium part by weight again; Every 10M when spraying
2The area usage quantity is 20～100g mycelium and conidium diluent.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101637102B (en) * | 2009-05-20 | 2014-01-22 | 董婉如 | Method for field culture of cordyceps sinensis and vegetation recovery in alpine-cold cordyceps growing areas |
| CN104145712A (en) * | 2014-07-19 | 2014-11-19 | 福建农林大学 | Method for using bamboo forest to cultivate cordyceps sobolifera |
| CN104920066A (en) * | 2015-05-28 | 2015-09-23 | 生展生物科技股份有限公司 | Method for improving infection rate of hosts of parasites of cordyceps sinensis |
| TWI611018B (en) * | 2016-03-22 | 2018-01-11 | 廣東東陽光藥業有限公司 | Method and system of cultivating ophiocordyceps sinensis |
| CN112088717A (en) * | 2020-10-14 | 2020-12-18 | 青海大学 | Method for cultivating cordyceps sinensis in field |
| CN117063780A (en) * | 2023-10-17 | 2023-11-17 | 四川朕源生物科技有限公司 | Cordyceps sinensis ascospore harvesting culture medium and harvesting method thereof |
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2006
- 2006-11-08 CN CN 200610123384 patent/CN1948457A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101637102B (en) * | 2009-05-20 | 2014-01-22 | 董婉如 | Method for field culture of cordyceps sinensis and vegetation recovery in alpine-cold cordyceps growing areas |
| CN104145712A (en) * | 2014-07-19 | 2014-11-19 | 福建农林大学 | Method for using bamboo forest to cultivate cordyceps sobolifera |
| CN104145712B (en) * | 2014-07-19 | 2016-01-27 | 福建农林大学 | A kind of method utilizing bamboo forest cultivation cicada fungus |
| CN104920066A (en) * | 2015-05-28 | 2015-09-23 | 生展生物科技股份有限公司 | Method for improving infection rate of hosts of parasites of cordyceps sinensis |
| CN104920066B (en) * | 2015-05-28 | 2018-10-26 | 生展生物科技股份有限公司 | The method for improving host of Cordyceps sinensis infection rate |
| TWI611018B (en) * | 2016-03-22 | 2018-01-11 | 廣東東陽光藥業有限公司 | Method and system of cultivating ophiocordyceps sinensis |
| CN112088717A (en) * | 2020-10-14 | 2020-12-18 | 青海大学 | Method for cultivating cordyceps sinensis in field |
| CN117063780A (en) * | 2023-10-17 | 2023-11-17 | 四川朕源生物科技有限公司 | Cordyceps sinensis ascospore harvesting culture medium and harvesting method thereof |
| CN117063780B (en) * | 2023-10-17 | 2023-12-26 | 四川朕源生物科技有限公司 | Cordyceps sinensis ascospore harvesting culture medium and harvesting method thereof |
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