CN102550489B - Artificial propagation method for Ooencyrtus kuvanae - Google Patents
Artificial propagation method for Ooencyrtus kuvanae Download PDFInfo
- Publication number
- CN102550489B CN102550489B CN 201210000679 CN201210000679A CN102550489B CN 102550489 B CN102550489 B CN 102550489B CN 201210000679 CN201210000679 CN 201210000679 CN 201210000679 A CN201210000679 A CN 201210000679A CN 102550489 B CN102550489 B CN 102550489B
- Authority
- CN
- China
- Prior art keywords
- ovum
- encyrtid
- large moth
- days
- adult
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention discloses an artificial propagation method for Ooencyrtus kuvanae. A caesarean egg of a tussah is used as a substitute host, the treated caesarean egg is used for inoculating Ooencyrtus kuvanae, and by culturing at normal temperature, a descendant adult fly with properties the same as that of the fly cultured from an original host egg can be obtained. By the method, Ooencyrtus kuvanae can be propagated indoors on large scale, and a practical technical means for biological control of gypsymoth in forests is provided.
Description
Technical field
The present invention relates to insect and breed the field, relate in particular to the artificial fecundation method of a kind of large moth ovum encyrtid.
Background technology
Large moth ovum encyrtid (Ooencyrtus kuwanai Howard, Hymenoptera: the Encytidae) ovum of main parasitic gypsymoth (Lymantria dispar Linnaeus) is also the parasitic wasp of the lepidoptera pest ovum such as pine caterpillars (Dendrolimus spp.) and leucoma candida (Stilpnotia salicis Linnaeus).Domestic known distribution in Jilin, the province such as Liaoning, Beijing, Hunan, Sichuan, be distributed in Japan abroad, once introduce many state control gypsymoths on the ground such as West Europe, north African and North America.
Gypsymoth is all 1 year generation all over the world, April in ovum phase from August to next year.Large moth ovum encyrtid all can be parasitic to any developmental stage of gypsymoth ovum, and can successfully complete the history of life.Development duration from the ovum to the adult eclosion is 21 days under 25 ℃ of conditions, is 14 days in the time of 30 ℃.At Connecticut, USA, this honeybee can complete for 4～5 generations every year.In the warmer southern areas of weather, can complete 6～7 generations in 1 year.Generally, 1 the large moth ovum encyrtid that only sprouts wings in gypsymoth ovum.
Large moth ovum encyrtid is the Natural Enemies of gypsymoth ovum, but not high at some local parasitic rate, the parasitic rate in the Beijing area is 2% left and right.The indoor gypsymoth ovum that once adopted is bred large moth ovum encyrtid.But the gypsymoth ovum both had been not easy to obtain, and operate and certain harmfulness is arranged, so the method is difficult to the batch breeding of large moth ovum encyrtid.
Tussah (Antheraea pemyi Guerin-Meneville) ovum can be used for breeding the egg parasitoidses such as white instep anastatus japonicus (Pseudanstatus albitarsis Ashmead) and multiple oophagous trichogrammae.Egg of oak silkworm host as an alternative may have stronger parasitic potential than other Hosts ovum to parasitic wasp, and the advantage aspect preservation and rejuvenation.The correlation techniques such as the acquisition of egg of oak silkworm, preservation, inoculation are comparative maturity also.Do not find that in the open air large moth ovum encyrtid can parasitic egg of oak silkworm, up to the present, also and have no favourable report with the large moth ovum encyrtid of egg of oak silkworm breeding.
Summary of the invention
Technical problem to be solved by this invention is to provide the artificial fecundation method of a kind of large moth ovum encyrtid, uses the method the large moth ovum of indoor large-scale breeding encyrtid, to provide the technological means of practicality for woodland biological control gypsymoth.
In order to solve the problems of the technologies described above, the present invention takes following technical scheme:
The artificial fecundation method of a kind of large moth ovum encyrtid is characterized in that comprising the steps:
(a) tussah chrysalis is sprouted wings after ovum cut open the belly and get ovum ,-4～-2 ℃ of lower freezings 15～60 days;
(b) ovum after freezing was thawed under 5～12 ℃ 1～2 day;
(c) the inoculation box of putting into large moth ovum encyrtid adult of the ovum after thawing is inoculated, ovum is 3～5: 1 with the number ratio of large moth ovum encyrtid adult, and feed large moth ovum encyrtid adult take mass concentration as 15% hydromel, inoculation time is 3～5 days;
(d) the complete rear taking-up ovum of inoculation is cultivated under 22～26 ℃ and is sprouted wings large moth ovum encyrtid.
Technique scheme, can do following optimization:
(a) tussah chrysalis is sprouted wings after ovum cut open the belly and get ovum ,-3 ℃ of lower freezings 40 days;
(b) ovum after freezing was thawed under 10 ℃ 1.5 days;
(c) the inoculation box of putting into large moth ovum encyrtid adult of the ovum after thawing is inoculated, and ovum is 4: 1 with the number ratio of large moth ovum encyrtid adult, and feeds large moth ovum encyrtid adult take mass concentration as 15% hydromel, and inoculation time is 4 days;
(d) the complete rear taking-up ovum of inoculation is cultivated under 25 ℃ and is sprouted wings large moth ovum encyrtid.
The invention has the advantages that: the normal ovum that produces after the female worm of tussah sprouts wings can not be as the host of large moth ovum encyrtid, ovum after the present invention sprouts wings to the female worm of tussah is processed, make the ovum after processing can be used as the Hosts of large moth ovum encyrtid, thereby completed the mass artificial propagation of large moth ovum encyrtid.Statistics shows, the female individuals of the large moth ovum encyrtid of above-mentioned artificial propagation is larger than the female individuals of sprouting wings in gypsymoth ovum, in vigorous health, the male difference in size is not obvious, in addition, the large moth ovum encyrtid that the large moth ovum encyrtid of artificial propagation and gypsymoth ovum are sprouted wings is all not remarkable in aspect difference such as life-span, male and female sex ratios, illustrates that the filial generation of this artificial fecundation method breeding is very similar to the filial generation of large moth ovum encyrtid natural propagation.
Embodiment
Embodiment one: the acquisition of Hosts ovum
Utilize egg of oak silkworm as an alternative the host breed large moth ovum encyrtid.Buy female tussah chrysalis on market.Pupa is before use in 0～5 ℃ of preservation.Tussah chrysalis is put under 25 ℃ of room temperature conditions during use, it is sprouted wings for becoming moth.Then female one-tenth moth is dissected, obtained to dissect ovum, ovum is cleaned, dried, reject abnormal ovum and obtain normal ovum.
Embodiment two:
(a) tussah chrysalis is sprouted wings after ovum cut open the belly and get ovum ,-4 ℃ of lower freezings 15 days;
(b) with the transition 2 days of thawing in the climatic cabinate of the ovum after freezing under 5 ℃;
(c) the inoculation box of putting into large moth ovum encyrtid adult of the ovum after thawing is inoculated, ovum is 3: 1 (300 ovum of 100 large moth ovum encyrtid inoculations) with the number ratio of large moth ovum encyrtid adult, and feed large moth ovum encyrtid adult take mass concentration as 15% hydromel, inoculation time is 5 days;
(d) the complete rear taking-up ovum of inoculation is cultivated under 22 ℃, and sprouting wings about 19 days large moth ovum encyrtid, maximum 8 the large moth ovum encyrtids that sprout wings of ovum, 2 large moth ovum encyrtids of minimum emergence, 5.2 large moth ovum encyrtids of average every ovum emergence.
Embodiment three:
(a) tussah chrysalis is sprouted wings after ovum cut open the belly and get ovum ,-2 ℃ of lower freezings 60 days;
(b) with the transition 1 day of thawing in the climatic cabinate of the ovum after freezing under 12 ℃;
(c) the inoculation box of putting into large moth ovum encyrtid adult of the ovum after thawing is inoculated, ovum is 5: 1 (500 ovum of 100 large moth ovum encyrtid inoculations) with the number ratio of large moth ovum encyrtid adult, and feed large moth ovum encyrtid adult take mass concentration as 15% hydromel, inoculation time is 3 days;
(d) the complete rear taking-up ovum of inoculation is cultivated under 26 ℃, and sprouting wings about 17 days large moth ovum encyrtid, maximum 9 the large moth ovum encyrtids that sprout wings of ovum, 3 large moth ovum encyrtids of minimum emergence, 5.5 large moth ovum encyrtids of average every ovum emergence.
Embodiment four:
(a) tussah chrysalis is sprouted wings after ovum cut open the belly and get ovum ,-3 ℃ of lower freezings 40 days;
(b) with the transition 1.5 days of thawing in the climatic cabinate of the ovum after freezing under 10 ℃;
(c) the inoculation box of putting into large moth ovum encyrtid adult of the ovum after thawing is inoculated, ovum is 4: 1 (400 ovum of 100 large moth ovum encyrtid inoculations) with the number ratio of large moth ovum encyrtid adult, and feed large moth ovum encyrtid adult take mass concentration as 15% hydromel, inoculation time is 4 days;
(d) the complete rear taking-up ovum of inoculation is cultivated under 25 ℃, and sprouting wings about 18 days large moth ovum encyrtid, maximum 11 the large moth ovum encyrtids that sprout wings of ovum, 3 large moth ovum encyrtids of minimum emergence, 6 large moth ovum encyrtids of average every ovum emergence.
Embodiment five:
The Time dependent of egg of oak silkworm preservation under-4～-2 ℃ of conditions the success rate of large moth ovum encyrtid breeding.0～7 day (not containing 7 days) of preservation, egg of oak silkworm is perishable mouldy after taking out; 7～15 days (not containing 15 days) of preservation can breed large moth ovum encyrtid, but large moth ovum encyrtid breeding success rate is low, is 5%; Preservation 15～60 days, the one-tenth rate of large moth ovum encyrtid breeding reaches 80%, and effect is best.
Embodiment six:
Individual Size: each 30 of the moth ovum encyrtids female great and mighty or powerful that hatches in each 30 of the moth ovum encyrtids female great and mighty or powerful that egg of oak silkworm is hatched and gypsymoth ovum are measured respectively size, obtain data.Statistics shows, the female large moth ovum encyrtid individuality that the mean value of the large moth ovum encyrtid female individuals that of sprouting wings from egg of oak silkworm than sprouting wings in gypsymoth ovum is larger, significant difference; And the mean value of male size is than gypsymoth ovum the individual less than normal of the large moth ovum encyrtid that sprout wings, but difference is not remarkable.
Life-span: the large moth ovum encyrtid that the gypsymoth ovum is sprouted wings, drone life-span average out to 15.95 days, queen bee life-span average out to 20.94 days; The large moth ovum encyrtid that egg of oak silkworm is sprouted wings, drone life-span average out to 10.38 days, queen bee life-span average out to 15.01 days.Can satisfy the needs as the biological and ecological methods to prevent plant disease, pests, and erosion factor.
Male and female sex ratio: respectively the sprout wings male and female sex of the large moth ovum encyrtid of 1000 that of gypsymoth ovum and egg of oak silkworm is tested, show that egg of oak silkworm the sprout wings female and male ratio of the large moth ovum encyrtid that of the large moth ovum encyrtid that and gypsymoth ovum of sprouting wings does not have significant difference.
Claims (2)
1. the artificial fecundation method of a large moth ovum encyrtid, is characterized in that comprising the steps:
(a) cut open the belly after tussah chrysalis is sprouted wings and get ovum ,-4～-2 ℃ of lower freezings 15～60 days;
(b) ovum after freezing was thawed under 5～12 ℃ 1～2 day;
(c) the inoculation box of putting into large moth ovum encyrtid adult of the ovum after thawing is inoculated, ovum is 3～5: 1 with the number ratio of large moth ovum encyrtid adult, and feed large moth ovum encyrtid adult take mass concentration as 15% hydromel, inoculation time is 3～5 days;
(d) the complete rear taking-up ovum of inoculation is cultivated under 22～26 ℃ and is sprouted wings large moth ovum encyrtid.
2. the artificial fecundation method of large moth ovum encyrtid as claimed in claim 1 is characterized in that:
(a) cut open the belly after tussah chrysalis is sprouted wings and get ovum ,-3 ℃ of lower freezings 40 days;
(b) ovum after freezing was thawed under 10 ℃ 1.5 days;
(c) the inoculation box of putting into large moth ovum encyrtid adult of the ovum after thawing is inoculated, and ovum is 4: 1 with the number ratio of large moth ovum encyrtid adult, and feeds large moth ovum encyrtid adult take mass concentration as 15% hydromel, and inoculation time is 4 days;
(d) the complete rear taking-up ovum of inoculation is cultivated under 25 ℃ and is sprouted wings large moth ovum encyrtid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210000679 CN102550489B (en) | 2012-01-04 | 2012-01-04 | Artificial propagation method for Ooencyrtus kuvanae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210000679 CN102550489B (en) | 2012-01-04 | 2012-01-04 | Artificial propagation method for Ooencyrtus kuvanae |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102550489A CN102550489A (en) | 2012-07-11 |
| CN102550489B true CN102550489B (en) | 2013-11-06 |
Family
ID=46398146
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201210000679 Expired - Fee Related CN102550489B (en) | 2012-01-04 | 2012-01-04 | Artificial propagation method for Ooencyrtus kuvanae |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102550489B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2728337C1 (en) * | 2019-10-08 | 2020-07-29 | Федеральное бюджетное учреждение "Всероссийский научно-исследовательский институт лесоводства и механизации лесного хозяйства (ФБУ ВНИИЛМ) | Method for cultivation of laboratory culture of entomophage egg parasite ooencyrtus kuvanae (howard) |
| RU2730035C1 (en) * | 2019-10-08 | 2020-08-14 | Федеральное бюджетное учреждение "Всероссийский научно-исследовательский институт лесоводства и механизации лесного хозяйства (ФБУ ВНИИЛМ) | Method for mass rearing of egg parasite ooencyrtus kuvanae (howard) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103918613B (en) * | 2014-04-23 | 2016-04-27 | 安徽师范大学 | A method for parasitic wasp artificial propagation in pupa time, and raise bees box and preparation method |
| CN105145495B (en) * | 2015-09-10 | 2017-10-27 | 延安大学 | Jujube tree steals the artificial breeding method of poison moth |
| KR102032946B1 (en) * | 2017-09-06 | 2019-10-16 | 순천대학교 산학협력단 | Artificial egg bag for rearing Oorencyrtus nezarae |
| KR102086772B1 (en) * | 2017-09-06 | 2020-03-09 | 순천대학교 산학협력단 | A method for artificial rearing Oorencyrtus nezarae |
| CN107455343A (en) * | 2017-09-09 | 2017-12-12 | 中国农业科学院植物保护研究所 | Predatory Mites are cut open the belly the acquisition methods of ovum |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05103563A (en) * | 1991-10-11 | 1993-04-27 | Sumitomo Chem Co Ltd | Method for rearing parasitic bee |
| FR2835701A1 (en) * | 2002-02-14 | 2003-08-15 | Univ Rennes | Rearing the parasitic wasp Trybliographa rapae, for use in biological control of Delia flies, especially cabbage flies, by producing fly larvae in cruciferous vegetables then infesting with wasp larvae |
| CN1279809C (en) * | 2005-01-26 | 2006-10-18 | 北京市农林科学院 | Artificial breeding and producing method for trichogramma |
-
2012
- 2012-01-04 CN CN 201210000679 patent/CN102550489B/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2728337C1 (en) * | 2019-10-08 | 2020-07-29 | Федеральное бюджетное учреждение "Всероссийский научно-исследовательский институт лесоводства и механизации лесного хозяйства (ФБУ ВНИИЛМ) | Method for cultivation of laboratory culture of entomophage egg parasite ooencyrtus kuvanae (howard) |
| RU2730035C1 (en) * | 2019-10-08 | 2020-08-14 | Федеральное бюджетное учреждение "Всероссийский научно-исследовательский институт лесоводства и механизации лесного хозяйства (ФБУ ВНИИЛМ) | Method for mass rearing of egg parasite ooencyrtus kuvanae (howard) |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102550489A (en) | 2012-07-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102550489B (en) | Artificial propagation method for Ooencyrtus kuvanae | |
| CN102318571B (en) | Culture method of hybrid new variety of Argopecten irradias and Argopecten purpuratus | |
| CN103168751B (en) | Expanding propagation method for aphidius gifuensis ashmeads | |
| CN100388884C (en) | Method for breeding pyemote, and its culturing vessel, and method for releasing same | |
| CN105766803B (en) | Egg of oak silkworm mixes breeding trichogramma dendrolimi and Trichogramma Ostriniae method for host | |
| Tikader et al. | Muga silkworm, Antheraea assamensis (Lepidoptera: Saturniidae)-an overview of distribution, biology and breeding. | |
| CN102742532A (en) | Cultivation method of novel strain of crassostrea gigas with pure purple left shells | |
| CN103004704A (en) | Method for breeding Scleroderma guani Xiao et Wu by using barley pests as intermediate hosts | |
| CN103141443B (en) | A kind of regulate and control the method for beet webworm Ah lattice ichneumon wasp diapause | |
| CN102144621A (en) | Induced scleroderma guani breeding method | |
| CN102870742A (en) | Large-scale artificial feeding method for eretmocerus hayati | |
| CN102669086A (en) | Novel method for preventing hyphantria cunea by means of Chouioia cunea Yang | |
| CN102524193A (en) | Large-scale raising method of dominant parasitoid wasps of liriomyza sativae | |
| CN108450418A (en) | A kind of method of biological control fall webworms | |
| Zheng et al. | Development and reproduction of the hoverfly Eupeodes corollae (Diptera: Syrphidae) | |
| CN109042542B (en) | Method for breeding propylaea japonica by using semi-artificial feed | |
| CN102440185A (en) | Fast culturing method for lily virus-free seed ball | |
| CN103349182B (en) | Feed for artificially feeding trichoferus campestris larvae and application thereof | |
| CN104823889A (en) | Breeding method for scallop hybridization breeding | |
| Gupta et al. | Life history of Aphis pomi De Geer (green apple aphid) on apple plantations in Jammu Province, J&K, India | |
| CN103704070B (en) | The biological control method of a kind of mythimna separata | |
| CN106508813B (en) | Wild-imitating propagation method for caesalpinia punctata | |
| CN103931566A (en) | Method for inducing diapause of oriental tobacco budworms | |
| CN102972353B (en) | Three-dimensional scale mixed feeding method for ophraella communa and epiblema strenuana | |
| EP4003021A1 (en) | Feed stuff for beneficial organisms that can be used in integrated pest management |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131106 Termination date: 20150104 |
|
| EXPY | Termination of patent right or utility model |