CN103931566A - Method for inducing diapause of oriental tobacco budworms - Google Patents
Method for inducing diapause of oriental tobacco budworms Download PDFInfo
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- CN103931566A CN103931566A CN201410170218.1A CN201410170218A CN103931566A CN 103931566 A CN103931566 A CN 103931566A CN 201410170218 A CN201410170218 A CN 201410170218A CN 103931566 A CN103931566 A CN 103931566A
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Abstract
The invention provides a method for inducing diapause of oriental tobacco budworms, and belongs to the technical field of insect pest prevention and control. The method comprises the following steps that (1) newly hatched larvae of the oriental tobacco budworms are bred to three ages in a culture dish with the diameter being 10-15 cm by artificial feed, then the oriental tobacco budworms are bred in a 1.2cm*10cm glass test tube, the temperature is pre-controlled to be 20-25 DEG C, and the light dark ratio is 8-11: 13-16, the light intensity is 25-35%, and the budworms are in standby application; (2) the budworms in the (1) step are transferred to a manual climatic box to be bred for 10-12 days, the temperature of the manual climatic box is 4-5 DEG C, the relative humidity of the manual climatic box is 78-86%, then the budworms are bred in a 0 DEG C refrigerator for 15-18 days, processing is repeated two to three times, and standby application is conducted; (3) at last, the budworms which are processed in the (2) step are transferred to a 25-32 DEG C box to be bred for three to four days, then normal breeding is conducted, and diapause of the oriental tobacco budworms can be achieved. According to the method for inducing diapause of the oriental tobacco budworms, operation is convenient, cost is low, the effect is good, and any bad effect can not happen.
Description
Technical field
The invention belongs to insect prevention and control technical field, be specifically related to a kind of method of inducing oriental tobacco budworm diapause.
Background technology
Oriental tobacco budworm has another name called the real noctuid of cigarette or tobacco budworm, belongs to Lepidoptera, Noctuidae in classification.Oriental tobacco budworm distributes extensively, feeding habits are assorted, fertility is strong, strong stress resistance, also has the characteristic of diapause, the most serious to endanger tobacco.There is harm seriously in many places tobacco producing region in recent years, as Guizhou, Sichuan, Central China and the Yellow River and Huai He River cigarette district.Endangered by oriental tobacco budworm, general time tobacco lost units 5%-10%, outbreak year part can exceed 15%.The harm of oriental tobacco budworm is that blade, flower, flower bud, fruit of its larval feeding host etc. causes, and therefore instar larvae and generation directly have influence on the extent of injury to crop.Because each department host's difference, and different regions different geographic populations the many factors such as there are differences, and causes different regions also different to the report of oriental tobacco budworm instar larvae.When ambient environmental conditions is suitable, the insect breeding of can continuously growing, survives when unfavorable to it when condition changes, and insect can be stagnated and be grown, and this state is exactly diapause.Insect enters diapause need to accept specific signal, and sort signal generally derives from environment.Temperature is the key factor that induction insect enters diapause, and mostly laboratory is to utilize temperature to induce insect.
In diapause at the beginning, for fear of the unfavorable damage that bring of low temperature to body during this, can there is to improve cold resistant metabolize regulate in insect in body.Studies have found that, polyalcohol (glycerine, mannitol, sorbitol, threitol etc.) and carbohydrate (trehalose, sucrose, fructose, glucose etc.) accumulation are obviously, thereby these variations can help insect to improve cold tolerance by reducing freezing point and supercooling point, in the time that polyalcohol concentration culminates, insect has just had cold tolerance.Recently some researchs find, the drought resisting factor of insect also comprises some albumen and amino acid, and many diapause insects can produce antifreeze protein (AFP), antifreeze peptide and can reduce the supercooling point of body fluid.But find through more deep concrete research, these antifreeze proteins can not make insect improve frost resistance, but by being adsorbed onto the ice crystal surface of firm formation, stop the growth of ice-nucleus, to reduce and stable supercooling state.Although arrest of development during Insect Diapause, diapause and metabolism are connected with breeding, and after diapause, the biological property such as the life-span of insect, fecundity and filial generation growth and diapause generation and diapause duration have relation.The time-to-live of the generation of diapause after to Insect Diapause is influential, and the life-span generally living through after the individual diapause of diapause can be shorter than there is no the individuality of diapause, and diapause duration also can have influence on the life after Insect Diapause.Although some insect experience termination of diapause, if be subject to the impact again of envirment factor, can enter diapause again.Diapause not only exerts an influence to mother generation of diapause, and also can affect to some extent its filial generation, for example on reduce filial generation developmental rate, affect the fecundity of filial generation.The present invention carries out diapause induction by certain inductive technology to oriental tobacco budworm, destroys its physiological function, reduce its filial generation developmental rate, affect the fecundity of filial generation, the prevention and control of oriental tobacco budworm are had great importance.
Summary of the invention
For the above-mentioned problems in the prior art, the object of the invention is to design provides a kind of technical scheme of method of inducing oriental tobacco budworm diapause, can reach prevention and control insect object by simple processing, and the method is easy to operate, cost is low, effective, can not produce any adverse effect.
A kind of described method of inducing oriental tobacco budworm diapause, is characterized in that comprising the following steps:
1) oriental tobacco budworm newly hatched larvae is that 10-15cm culture dish artificial feed were supported to 3 ages at diameter, then in 1.2cm × 10cm teat glass, raises, and it is 8-11:13-16 that temperature pre-control is made as 20-25 DEG C, Light To Dark Ratio, and luminous intensity is 25-35%, for subsequent use;
2) then insect in step 1) is moved to 4-5 DEG C, in climatic cabinate that relative moisture is 78-86%, raise 10-12 days, be placed in the refrigerator of 0 DEG C again and raise 15-18 days, reprocessing 2-3 time, for subsequent use;
3) finally by step 2) in insect after treatment move in 25-32 DEG C of case and raise 3-4d, then normally cultivate, can make oriental tobacco budworm diapause.
A kind of described method of inducing oriental tobacco budworm diapause, its feature is in step 1): in raising temperature pre-control at 21-24 DEG C, preferably 22-23 DEG C.
A kind of described method of inducing oriental tobacco budworm diapause, is characterized in that in step 1): Light To Dark Ratio is 9-10:12-15.
A kind of described method of inducing oriental tobacco budworm diapause, is characterized in that in step 1): luminous intensity is 28-30%.
A kind of described method of inducing oriental tobacco budworm diapause, is characterized in that step 2) in: climatic cabinate temperature is 4.5 DEG C, and relative moisture is 80-85%, and feeding time is 11 days.
A kind of described method of inducing oriental tobacco budworm diapause, is characterized in that step 2) in: the refrigerator that is placed in again 0 DEG C is raised 16-17 days.
A kind of described method of inducing oriental tobacco budworm diapause, is characterized in that in step 3): raising temperature is 28-30 DEG C.
A kind of above-mentioned method of inducing oriental tobacco budworm diapause, utilize different variations in temperature to stimulate the physiological function of destroying insect, the diapause of induction oriental tobacco budworm, shorten insect bulk life time and destroy its reproduction ability, with reduce its filial generation developmental rate, affect the fecundity of its filial generation, can reach prevention and control insect object by simple processing, and the method is easy to operate, cost is low, effective, can not produce any adverse effect.
Embodiment
The present invention is further illustrated in conjunction with specific embodiments more below.
Embodiment 1
Oriental tobacco budworm newly hatched larvae is that 10cm culture dish artificial feed were supported to 3 ages at diameter, then in 1.2cm × 10cm teat glass, raises, and it is 10:14 that temperature pre-control is made as 20 DEG C, Light To Dark Ratio, and luminous intensity is 33%.Then insect is moved to 4 DEG C, in climatic cabinate that relative moisture is 78%, process 10 days, be placed in the refrigerator of 0 DEG C again and process 15 days, reprocessing 2 times, finally moves to insect after treatment at 25 DEG C and processes 3d.Then normally cultivate, can make oriental tobacco budworm diapause.
Embodiment 2
Oriental tobacco budworm newly hatched larvae is that 15m culture dish artificial feed were supported to 3 ages at diameter, then in 1.2cm × 10cm teat glass, raises, and it is 10:14 that temperature pre-control is made as 25 DEG C, Light To Dark Ratio, and luminous intensity is 31%.Then insect is moved to 4 DEG C, in climatic cabinate that relative moisture is 86%, process 10 days, be placed in the refrigerator of 0 DEG C again and process 15 days, reprocessing 3 times, finally moves to insect after treatment at 32 DEG C and processes 3d.Then normally cultivate, can make oriental tobacco budworm diapause.
Embodiment 3
Oriental tobacco budworm newly hatched larvae is that 13m culture dish artificial feed were supported to 3 ages at diameter, then in 1.2cm × 10cm teat glass, raises, and it is 10:14 that temperature pre-control is made as 21 DEG C, Light To Dark Ratio, and luminous intensity is 29%.Then insect is moved to 4 DEG C, in climatic cabinate that relative moisture is 80%, process 10 days, be placed in the refrigerator of 0 DEG C again and process 15 days, reprocessing 3 times, finally moves to insect after treatment at 27 DEG C and processes 3d.Then normally cultivate, can make oriental tobacco budworm diapause.
Embodiment 4
Oriental tobacco budworm newly hatched larvae is that 12m culture dish artificial feed were supported to 3 ages at diameter, then in 1.2cm × 10cm teat glass, raises, and it is 10:14 that temperature pre-control is made as 22 DEG C, Light To Dark Ratio, and luminous intensity is 27%.Then insect is moved to 4 DEG C, in climatic cabinate that relative moisture is 82%, process 10 days, be placed in the refrigerator of 0 DEG C again and process 15 days, reprocessing 2 times, finally moves to insect after treatment at 29 DEG C and processes 3d.Then normally cultivate, can make oriental tobacco budworm diapause.
Embodiment 5
Oriental tobacco budworm newly hatched larvae is that 11m culture dish artificial feed were supported to 3 ages at diameter, then in 1.2cm × 10cm teat glass, raises, and it is 10:14 that temperature pre-control is made as 23 DEG C, Light To Dark Ratio, and luminous intensity is 35%.Then insect is moved to 4 DEG C, in climatic cabinate that relative moisture is 84%, process 10 days, be placed in the refrigerator of 0 DEG C again and process 15 days, reprocessing 3 times, finally moves to insect after treatment at 30 DEG C and processes 3d.Then normally cultivate, can make oriental tobacco budworm diapause.
Embodiment 6
Oriental tobacco budworm newly hatched larvae is that 10cm culture dish artificial feed were supported to 3 ages at diameter, then in 1.2cm × 10cm teat glass, raises, and it is 10:14 that temperature pre-control is made as 24 DEG C, Light To Dark Ratio, and luminous intensity is 25%.Then insect is moved to 4 DEG C, in climatic cabinate that relative moisture is 85%, process 10 days, be placed in the refrigerator of 0 DEG C again and process 15 days, reprocessing 2 times, finally moves to insect after treatment at 31 DEG C and processes 3d.Then normally cultivate, can make oriental tobacco budworm diapause.
Further illustrate beneficial effect of the present invention by test below
Table 1 embodiment 1-6 processes lower oriental tobacco budworm diapause result
| Process | Worm pupa number | The not initial body weight of diapause pupa (mg) | The initial body weight of diapause pupa (mg) |
| Embodiment 1 | 50 | 273.22±5.95 | 238.38±6.27 |
| Embodiment 2 | 50 | 276.04±4.38 | 231.41±4.88 |
| Embodiment 3 | 50 | 283.30±3.83 | 234.47±2.5 |
| Embodiment 4 | 50 | 284.45±2.16 | 239.28±4.13 |
| Embodiment 5 | 50 | 282.16±3.27 | 230.26±5.57 |
| Embodiment 6 | 50 | 279.42±4.10 | 235.35±3.15 |
Note: in table, data are mean value ± standard error (Mean ± SE)
Result of the test shows: embodiment 1-6 oriental tobacco budworm after treatment grows and is all obviously suppressed, and can produce diapause effect to it, and wherein embodiment 2 is better than embodiment 1, and embodiment 3 is better than embodiment 4, and embodiment 5 is better than embodiment 6.
Claims (7)
1. induce a method for oriental tobacco budworm diapause, it is characterized in that comprising the following steps:
1) oriental tobacco budworm newly hatched larvae is that 10-15cm culture dish artificial feed were supported to 3 ages at diameter, then in 1.2cm × 10cm teat glass, raises, and it is 8-11:13-16 that temperature pre-control is made as 20-25 DEG C, Light To Dark Ratio, and luminous intensity is 25-35%, for subsequent use;
2) then insect in step 1) is moved to 4-5 DEG C, in climatic cabinate that relative moisture is 78-86%, raise 10-12 days, be placed in the refrigerator of 0 DEG C again and raise 15-18 days, reprocessing 2-3 time, for subsequent use;
3) finally by step 2) in insect after treatment move in 25-32 DEG C of case and raise 3-4d, then normally cultivate, can make oriental tobacco budworm diapause.
2. a kind of method of inducing oriental tobacco budworm diapause as claimed in claim 1, its feature is in step 1): in raising temperature pre-control at 21-24 DEG C, preferably 22-23 DEG C.
3. a kind of method of inducing oriental tobacco budworm diapause as claimed in claim 1, is characterized in that in step 1): Light To Dark Ratio is 9-10:12-15.
4. a kind of method of inducing oriental tobacco budworm diapause as claimed in claim 1, is characterized in that in step 1): luminous intensity is 28-30%.
5. a kind of method of inducing oriental tobacco budworm diapause as claimed in claim 1, is characterized in that step 2) in: climatic cabinate temperature is 4.5 DEG C, and relative moisture is 80-85%, and feeding time is 11 days.
6. a kind of method of inducing oriental tobacco budworm diapause as claimed in claim 1, is characterized in that step 2) in: the refrigerator that is placed in again 0 DEG C is raised 16-17 days.
7. a kind of method of inducing oriental tobacco budworm diapause as claimed in claim 1, is characterized in that in step 3): raising temperature is 28-30 DEG C.
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| CN104255669A (en) * | 2014-09-24 | 2015-01-07 | 中国农业科学院植物保护研究所 | Diapause induction method of harmonia dimidiata |
| CN104351140A (en) * | 2014-09-24 | 2015-02-18 | 中国农业科学院植物保护研究所 | Leis dimidiata diapause relieving method |
| CN108157399A (en) * | 2017-12-11 | 2018-06-15 | 常州市协旺纺织品有限公司 | It is a kind of to be used to prevent preparation of oriental tobacco budworm and preparation method thereof |
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| CN104351140A (en) * | 2014-09-24 | 2015-02-18 | 中国农业科学院植物保护研究所 | Leis dimidiata diapause relieving method |
| CN108157399A (en) * | 2017-12-11 | 2018-06-15 | 常州市协旺纺织品有限公司 | It is a kind of to be used to prevent preparation of oriental tobacco budworm and preparation method thereof |
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