CN108157399A - It is a kind of to be used to prevent preparation of oriental tobacco budworm and preparation method thereof - Google Patents
It is a kind of to be used to prevent preparation of oriental tobacco budworm and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to pest control technical fields, and in particular to a kind of to be used to prevent preparation of oriental tobacco budworm and preparation method thereof.The present invention is for preventing the preparation of oriental tobacco budworm mainly based on induction, wherein fragrance matter is mainly chilli powder, larva main parasitic is in chilli powder, it is very big to its attraction so as to the fragrance of capsicum, and oriental tobacco budworm has phototaxis, preparation is made to shine using fluorescein, adult also has taxis to molasses simultaneously, enhance the attracting performance of preparation, so as to make oriental tobacco budworm parasitism, look for food in preparation, in addition, preparation also has a kind of trifoliate jewelvine extract that can kill oriental tobacco budworm substance, so as to reduce the amount of oriental tobacco budworm, quality and the safety of cigarette are improved.The present invention uses raw material, wherein sucrose can be absorbed by plants in glucide, plant extracts has its smoothly degradation pathway in nature, environmental pollution is small, there is specificity simultaneously, to higher mammal and pest natural enemy safety, very little is influenced on pest natural enemy, harmful organism is not easy to produce drug resistance.
Description
Technical field
The invention belongs to pest control technical fields, and in particular to a kind of preparation and its preparation side for being used to prevent oriental tobacco budworm
Method.
Background technology
Oriental tobacco budworm also known as Heliothis assulta or tobacco budworm belong to Lepidoptera, Noctuidae in classification.Oriental tobacco budworm distribution is wide, feeding habits are miscellaneous,
Reproductive capacity is strong, resistance is strong, also the characteristic with diapause, the most serious to endanger tobacco.Many places tobacco producing region occurs in recent years
Harm is serious, such as Guizhou, Sichuan, Central China and the Yellow River and Huai He River cigarette district.It is endangered by oriental tobacco budworm, general time tobacco lost units 5% ~ 10%,
Outbreak year part can be more than 15%.Caused by the harm of oriental tobacco budworm is blade, flower, flower bud, fruit of its larval feeding host etc., because
This instar larvae and generation directly influence the extent of injury to crop.Because each department host is different, and different regions
Different geographic populations, which have differences, waits many factors, causes different regions also different to the report of oriental tobacco budworm instar larvae.Work as week
It is suitable to enclose environmental condition, insect can continuously growth and development breeding, when condition, which changes, survives unfavorable to it, insect
Meeting growth arrest development, this state is exactly diapause.Insect, which enters diapause, to be needed to receive specific signal, and this signal generally comes
Derived from environment.Temperature is an important factor for induction insect enters diapause, and laboratory is mostly that insect is induced using temperature.
In diapause at the beginning, insect can occur in vivo in order to avoid the unfavorable damage brought of the low temperature to body during this
Improve the metabolism adjustment of winter resistance.Studies have found that polyalcohol（Glycerine, mannitol, D-sorbite, threitol etc.）And carbohydrate
（Trehalose, sucrose, fructose, glucose etc.）Accumulation is apparent, these variations can be by reducing freezing point and supercooling point so as to help
Insect is helped to improve cold tolerance, when polyhydric alcohol concentration culminates, insect is just provided with cold tolerance.Recently some research hairs
Existing, the drought resisting factor of insect also includes some albumen and amino acid, and many diapause insects can generate antifreeze protein（AFP）, it is anti-
The supercooling point of body fluid can be reduced by freezing peptide.But pass through deeper into specific the study found that these antifreeze proteins can not make
Insect improves frost resistance, but by being adsorbed onto newly formed ice crystal surface, the growth of ice-nucleus is prevented, to reduce and stablize supercooling
But state.Although arrest of development during Insect Diapause, diapause is connected with being metabolized and breeding, the longevity of insect after diapause
Biological characteristics and the diapause generations such as life, fecundity and Offspring's deyelopment and diapause duration have relationship.The generation of diapause is to elder brother
Time-to-live after worm diapause is influential, and generally living through the service life after the individual diapause of diapause can be than of no diapause
Body is short, and diapause duration also influences whether the life after Insect Diapause.Although some insects experience and termination of diapause, but if by
To the influence again of envirment factor, diapause can be again introduced into.Diapause not only has an impact, but also to its filial generation female generation of diapause
Also it can be influenced, such as on reducing the developmental rate of filial generation, influencing the fecundity of filial generation.The present invention passes through certain induction skill
Art carries out Diapause inducement to oriental tobacco budworm, destroys its physiological function, reduces the developmental rate of its filial generation, influences the fecundity of filial generation,
Have great importance to the prevention and control of oriental tobacco budworm.
It is at present chemical prevention to oriental tobacco budworm prevention main means, chemical prevention not only largely kills pest natural enemy, enhancing
Pest resistance to insecticide pollutes environment, and agricultural chemical residue in tobacco amount is caused to increase, and seriously affects quality and the safety of cigarette.With
People to environment and the pay attention to day by day of health, tobacco leaf safety is widely paid close attention to, therefore, to protect tobacco leaf, reductionization
The use for learning synthetic pesticide is target, and the road of one pollution-free, ecological, environmentally friendly oriental tobacco budworm prevention of exploration, which has become agricultural, to hold
The inevitable choice of supervention exhibition.
Invention content
The technical problems to be solved by the invention：It is chemical prevention for main means are prevented to oriental tobacco budworm at present, chemistry
Preparation not only pollutes environment, but also agricultural chemical residue in tobacco amount is caused to increase, the problem of seriously affecting quality and the safety of cigarette,
It provides a kind of for preventing preparation of oriental tobacco budworm and preparation method thereof.
In order to solve the above technical problems, the present invention is using technical solution as described below：
A kind of preparation for being used to prevent oriental tobacco budworm, is counted in parts by weight, and the preparation for being used to prevent oriental tobacco budworm is by following component
Composition：20 ~ 30 parts of water, 12 ~ 15 parts of wrappages, 10 ~ 15 parts of glucides, 7 ~ 8 parts of fragrance matters, 3 ~ 4 parts of plant extracts, 3 ~
4 parts of fluoresceins.
The wrappage is beta-cyclodextrin.
The glucide is in mass ratio 1：2 ~ 3, sucrose, honey are mixed, obtain mixture to get carbohydrate object
Matter.
The fragrance matter preparation method is that capsicum is put into stone mortar to smash to pieces, obtains chilli powder, counts in parts by weight, take 3 ~
5 parts of water, 2 ~ 4 portions of edible oils, 1 ~ 2 portion of chilli powder, first mix chilli powder, water, and be stirred with 100 ~ 150r/min
10 ~ 15min obtains mixture, then edible oil is heated to 155 DEG C, is directly added into mixture, when adding in 80 ~ 100r/
Min is stirred, and after storage for 24 hours, filtering obtains filter residue to get fragrance matter.
The plant extracts is that trifoliate jewelvine leaf is put into pulverizer to crush, and obtains crushed material, in mass ratio 1：3 ~ 4,
Crushed material, 1.0mol/L ethanol solutions are mixed, are divided into three parts, impregnates 3,5,7 d, filtering respectively, and merges 3 filters
Liquid obtains filtrate, and filtrate in 0.01 ~ 0.03MPa of pressure is concentrated under reduced pressure, concentrate is obtained, concentrate is freeze-dried,
Dried object is collected to get plant extraction liquid.
The preparation method of the fluorescein：It counts in parts by weight, takes 50 ~ 80 parts of Fresh Egg liquid, sodium glutamate 13 ~ 15
Part, 8 ~ 10 parts of glycerine, 1 ~ 2 part of potassium dihydrogen phosphate, 0.02 ~ 0.05 part of magnesium sulfate, 0.2 ~ 0.7 part of magnesium citrate, potato starch
12 ~ 15 parts, pH7.2 ± 0.2,121 DEG C sterilizing 20min, obtain egg medium, abalone luminous bacillus are seeded to egg medium,
It is cultivated at 28 ~ 32 DEG C of temperature, obtains the bacterium colony of bacterium diameter maximum, count in parts by weight, take 10 ~ 15 parts of peptone, beef extract 15 ~ 18
Part, 15 ~ 20 parts of sodium chloride, 20 ~ 23 parts, pH7.2 ± 0.2,121 DEG C sterilizing 20min of agar obtain solid medium, by bacterium diameter most
Big bacterium colony streak inoculation obtains bacterium colony, counts in parts by weight, take 10 ~ 15 parts of peptone, ox to solid medium culture 2 ~ 3 times
15 ~ 18 parts of meat extract, 15 ~ 20 parts, pH7.2 ± 0.2,121 DEG C sterilizing 20min of sodium chloride, obtains fluid nutrient medium, by colony inoculation extremely
Fluid nutrient medium increases bacterium in 20 ~ 23 DEG C of temperature, 30 ~ 40h of culture, obtains enrichment culture medium, by enrichment culture medium with 7000r/min into
Row centrifugation 20min, breaks bacterium wall through cell squeezer, carries out centrifugation 20min again with 8000r/min, obtain supernatant, in mass ratio
1：3, Na is added in into supernatant2P2O7Dialysis, obtain level-one dialysis enzyme solution, in mass ratio 1：6, it is added in level-one dialysis enzyme solution poly-
Ethylene glycol is dialysed, and is concentrated to get fluorescein.
The preparation method includes the following steps：
（1）Water, coating agent are subjected to Hybrid Heating, temperature rise to 45 ~ 50 DEG C, and be stirred 10 with 150 ~ 200r/min ~
15min obtains stirring mixture；
（2）Glucide, fragrance matter, plant extracts, fluorescein are mixed, and stirred with 150 ~ 200r/min
It mixes, stirring mixture is added in whipping process, obtain mixture to get for preventing the preparation of oriental tobacco budworm.
Compared with other methods, advantageous effects are the present invention：
（1）The present invention for preventing the preparation of oriental tobacco budworm mainly based on induction, wherein, fragrance matter is mainly chilli powder, children
Worm main parasitic is in chilli powder, so that the fragrance of capsicum is very big to its attraction, and oriental tobacco budworm has phototaxis, utilizes fluorescence
Element makes preparation shine, while adult also has taxis to molasses, enhances the attracting performance of preparation, and so as to make, oriental tobacco budworm is parasitic, looks for food
In preparation, in addition, preparation also has a kind of trifoliate jewelvine extract that can kill oriental tobacco budworm substance, so as to reduce the amount of oriental tobacco budworm,
Improve quality and the safety of cigarette.
（2）The present invention uses raw material, and wherein sucrose can be absorbed by plants in glucide, and plant extracts is in nature
There is its smoothly degradation pathway on boundary, and environmental pollution is small, while has specificity, right to higher mammal and pest natural enemy safety
Pest natural enemy influences very little, and harmful organism is not easy to produce drug resistance.
Figure of description
Fig. 1 is effective insect prevention rate line chart of the prevention oriental tobacco budworm medicament of the present invention.
Specific embodiment
Wrappage is beta-cyclodextrin.
Glucide is in mass ratio 1：2 ~ 3, sucrose, honey are mixed, obtain mixture to get glucide.
Fragrance matter preparation method is that capsicum is put into stone mortar to smash to pieces, obtains chilli powder, counts in parts by weight, takes 3 ~ 5 parts
Water, 2 ~ 4 portions of edible oils, 1 ~ 2 portion of chilli powder, first mix chilli powder, water, and 10 are stirred with 100 ~ 150r/min ~
15min obtains mixture, then edible oil is heated to 155 DEG C, is directly added into mixture, while add in while with 80 ~ 100r/min into
Row stirring, after storage for 24 hours, filtering obtains filter residue to get fragrance matter.
Plant extracts is that trifoliate jewelvine leaf is put into pulverizer to crush, and obtains crushed material, in mass ratio 1：3 ~ 4, by powder
Mince, 1.0mol/L ethanol solutions are mixed, be divided into three parts, respectively impregnate 3,5,7 d, filtering, and merge 3 filtrates, obtain
Filtrate in 0.01 ~ 0.03MPa of pressure is concentrated under reduced pressure, obtains concentrate, concentrate is freeze-dried by filtrate, collects
Dried object is to get plant extraction liquid.
The preparation method of fluorescein：It counts in parts by weight, takes 50 ~ 80 parts of Fresh Egg liquid, 13 ~ 15 parts of sodium glutamate is sweet
8 ~ 10 parts of oil, 1 ~ 2 part of potassium dihydrogen phosphate, 0.02 ~ 0.05 part of magnesium sulfate, 0.2 ~ 0.7 part of magnesium citrate, potato starch 12 ~ 15
Part, pH7.2 ± 0.2,121 DEG C of sterilizing 20min obtain egg medium, abalone luminous bacillus are seeded to egg medium, in temperature
It is cultivated at 28 ~ 32 DEG C, obtains the bacterium colony of bacterium diameter maximum, count in parts by weight, take 10 ~ 15 parts of peptone, 15 ~ 18 parts of beef extract, chlorine
Change 15 ~ 20 parts of sodium, 20 ~ 23 parts, pH7.2 ± 0.2,121 DEG C sterilizing 20min of agar obtain solid medium, by the bacterium of bacterium diameter maximum
Streak inoculation is fallen to solid medium culture 2 ~ 3 times, bacterium colony is obtained, counts in parts by weight, takes 10 ~ 15 parts of peptone, beef extract 15 ~
18 parts, 15 ~ 20 parts, pH7.2 ± 0.2,121 DEG C sterilizing 20min of sodium chloride obtain fluid nutrient medium, colony inoculation to liquid are trained
It supports base increasing bacterium and cultivates 30 ~ 40h for 20 ~ 23 DEG C in temperature, obtain enrichment culture medium, enrichment culture medium is centrifuged with 7000r/min
20min breaks bacterium wall through cell squeezer, carries out centrifugation 20min again with 8000r/min, obtains supernatant, in mass ratio 1：3, to
Na is added in supernatant2P2O7Dialysis, obtain level-one dialysis enzyme solution, in mass ratio 1：6, add in polyethylene glycol to level-one dialysis enzyme solution
Dialysis, concentrates to get fluorescein.
A kind of preparation for being used to prevent oriental tobacco budworm, is counted in parts by weight, and the preparation for being used to prevent oriental tobacco budworm is by following
Into being grouped as：20 ~ 30 parts of water, 12 ~ 15 parts of wrappages, 10 ~ 15 parts of glucides, 7 ~ 8 parts of fragrance matters, 3 ~ 4 parts of plant extracts
Object, 3 ~ 4 parts of fluoresceins.
A kind of preparation method for being used to prevent the preparation of oriental tobacco budworm as described in claim 1, which includes as follows
Step：
（1）Water, coating agent are subjected to Hybrid Heating, temperature rise to 45 ~ 50 DEG C, and be stirred 10 with 150 ~ 200r/min ~
15min obtains stirring mixture；
（2）Glucide, fragrance matter, plant extracts, fluorescein are mixed, and stirred with 150 ~ 200r/min
It mixes, stirring mixture is added in whipping process, obtain mixture to get for preventing the preparation of oriental tobacco budworm.
Embodiment 1
Wrappage is beta-cyclodextrin.
Glucide is in mass ratio 1：2, sucrose, honey are mixed, obtain mixture to get glucide.
Fragrance matter preparation method is that capsicum is put into stone mortar to smash to pieces, obtains chilli powder, counts in parts by weight, take 3 parts of water,
2 portions of edible oils, 1 portion of chilli powder, chilli powder, water are first mixed, and be stirred 10min with 100r/min, obtain mixture,
Edible oil is heated to 155 DEG C again, is directly added into mixture, is stirred when adding in 80r/min, after storage for 24 hours, mistake
Filter, obtains filter residue to get fragrance matter.
Plant extracts is that trifoliate jewelvine leaf is put into pulverizer to crush, and obtains crushed material, in mass ratio 1：3, it will crush
Object, 1.0mol/L ethanol solutions are mixed, and are divided into three parts, are impregnated 3,5,7 d, filtering respectively, and merge 3 filtrates, must be filtered
Filtrate in pressure 0.01MPa is concentrated under reduced pressure, obtains concentrate, concentrate is freeze-dried by liquid, collects dried object,
Up to plant extraction liquid.
The preparation method of fluorescein：It counts in parts by weight, takes 50 parts of Fresh Egg liquid, 13 parts of sodium glutamate, 8 parts of glycerine,
1 part of potassium dihydrogen phosphate, 0.02 part of magnesium sulfate, 0.2 part of magnesium citrate, 12 parts, pH7.2 ± 0.2,121 DEG C sterilizings of potato starch
20min obtains egg medium, and abalone luminous bacillus is seeded to egg medium, cultivated at 28 DEG C of temperature, and it is maximum to obtain bacterium diameter
Bacterium colony is counted in parts by weight, takes 10 parts of peptone, 15 parts of beef extract, 15 parts of sodium chloride, 20 parts, pH7.2 ± 0.2 of agar, and 121
DEG C sterilizing 20min, obtain solid medium, by the bacterium colony streak inoculation of bacterium diameter maximum to solid medium culture 2 times, obtain bacterium colony,
It counts in parts by weight, takes 10 parts of peptone, 15 parts of beef extract, 15 parts, pH7.2 ± 0.2,121 DEG C sterilizing 20min of sodium chloride are obtained
Colony inoculation to fluid nutrient medium is increased bacterium and cultivates 30h for 20 DEG C in temperature, obtained enrichment culture medium, will increase bacterium training by fluid nutrient medium
Nutrient solution carries out centrifugation 20min with 7000r/min, breaks bacterium wall through cell squeezer, carries out centrifugation 20min again with 8000r/min,
Supernatant, in mass ratio 1：3, Na is added in into supernatant2P2O7Dialysis, obtain level-one dialysis enzyme solution, in mass ratio 1：6, Xiang Yi
Grade dialysis enzyme solution adds in polyethylene glycol dialysis, concentrates to get fluorescein.
A kind of preparation for being used to prevent oriental tobacco budworm, is counted in parts by weight, and the preparation for being used to prevent oriental tobacco budworm is by following
Into being grouped as：20 parts of water, 12 parts of wrappages, 10 parts of glucides, 7 parts of fragrance matters, 3 parts of plant extracts, 3 parts of fluoresceins.
A kind of preparation method for being used to prevent the preparation of oriental tobacco budworm as described in claim 1, which includes as follows
Step：
（1）Water, coating agent are subjected to Hybrid Heating, temperature rise is stirred 10min to 45 DEG C, and with 150r/min, must stir
Mix mixture；
（2）Glucide, fragrance matter, plant extracts, fluorescein are mixed, and are stirred with 150r/min, is stirred
Stirring mixture is added in during mixing, obtains mixture to get for preventing the preparation of oriental tobacco budworm.
Embodiment 2
Wrappage is beta-cyclodextrin.
Glucide is in mass ratio 1：2, sucrose, honey are mixed, obtain mixture to get glucide.
Fragrance matter preparation method is that capsicum is put into stone mortar to smash to pieces, obtains chilli powder, counts in parts by weight, take 4 parts of water,
3 portions of edible oils, 1 portion of chilli powder, chilli powder, water are first mixed, and be stirred 13min with 125r/min, obtain mixture,
Edible oil is heated to 155 DEG C again, is directly added into mixture, is stirred when adding in 90r/min, after storage for 24 hours, mistake
Filter, obtains filter residue to get fragrance matter.
Plant extracts is that trifoliate jewelvine leaf is put into pulverizer to crush, and obtains crushed material, in mass ratio 1：3, it will crush
Object, 1.0mol/L ethanol solutions are mixed, and are divided into three parts, are impregnated 3,5,7 d, filtering respectively, and merge 3 filtrates, must be filtered
Filtrate in pressure 0.02MPa is concentrated under reduced pressure, obtains concentrate, concentrate is freeze-dried by liquid, collects dried object,
Up to plant extraction liquid.
The preparation method of fluorescein：It counts in parts by weight, takes 60 parts of Fresh Egg liquid, 14 parts of sodium glutamate, 9 parts of glycerine,
1 part of potassium dihydrogen phosphate, 0.03 part of magnesium sulfate, 0.6 part of magnesium citrate, 13 parts, pH7.2 ± 0.2,121 DEG C sterilizings of potato starch
20min obtains egg medium, and abalone luminous bacillus is seeded to egg medium, cultivated at 30 DEG C of temperature, and it is maximum to obtain bacterium diameter
Bacterium colony is counted in parts by weight, takes 13 parts of peptone, 16 parts of beef extract, 17 parts of sodium chloride, 22 parts, pH7.2 ± 0.2 of agar, and 121
DEG C sterilizing 20min, obtain solid medium, by the bacterium colony streak inoculation of bacterium diameter maximum to solid medium culture 2 times, obtain bacterium colony,
It counts in parts by weight, takes 13 parts of peptone, 16 parts of beef extract, 17 parts, pH7.2 ± 0.2,121 DEG C sterilizing 20min of sodium chloride are obtained
Colony inoculation to fluid nutrient medium is increased bacterium and cultivates 35h for 22 DEG C in temperature, obtained enrichment culture medium, will increase bacterium training by fluid nutrient medium
Nutrient solution carries out centrifugation 20min with 7000r/min, breaks bacterium wall through cell squeezer, carries out centrifugation 20min again with 8000r/min,
Supernatant, in mass ratio 1：3, Na is added in into supernatant2P2O7Dialysis, obtain level-one dialysis enzyme solution, in mass ratio 1：6, Xiang Yi
Grade dialysis enzyme solution adds in polyethylene glycol dialysis, concentrates to get fluorescein.
A kind of preparation for being used to prevent oriental tobacco budworm, is counted in parts by weight, and the preparation for being used to prevent oriental tobacco budworm is by following
Into being grouped as：25 parts of water, 14 parts of wrappages, 14 parts of glucides, 7 parts of fragrance matters, 3 parts of plant extracts, 3 parts of fluoresceins.
A kind of preparation method for being used to prevent the preparation of oriental tobacco budworm as described in claim 1, which includes as follows
Step：
（1）Water, coating agent are subjected to Hybrid Heating, temperature rise is stirred 13min to 47 DEG C, and with 185r/min, must stir
Mix mixture；
（2）Glucide, fragrance matter, plant extracts, fluorescein are mixed, and are stirred with 185r/min, is stirred
Stirring mixture is added in during mixing, obtains mixture to get for preventing the preparation of oriental tobacco budworm.
Embodiment 3
Wrappage is beta-cyclodextrin.
Glucide is in mass ratio 1：3, sucrose, honey are mixed, obtain mixture to get glucide.
Fragrance matter preparation method is that capsicum is put into stone mortar to smash to pieces, obtains chilli powder, counts in parts by weight, take 5 parts of water,
4 portions of edible oils, 2 portions of chilli powders, chilli powder, water are first mixed, and be stirred 15min with 150r/min, obtain mixture,
Edible oil is heated to 155 DEG C again, is directly added into mixture, is stirred when adding in 100r/min, after storage for 24 hours,
Filtering, obtains filter residue to get fragrance matter.
Plant extracts is that trifoliate jewelvine leaf is put into pulverizer to crush, and obtains crushed material, in mass ratio 1：4, it will crush
Object, 1.0mol/L ethanol solutions are mixed, and are divided into three parts, are impregnated 3,5,7 d, filtering respectively, and merge 3 filtrates, must be filtered
Filtrate in pressure 0.03MPa is concentrated under reduced pressure, obtains concentrate, concentrate is freeze-dried by liquid, collects dried object,
Up to plant extraction liquid.
The preparation method of fluorescein：It counts in parts by weight, takes 80 parts of Fresh Egg liquid, 15 parts of sodium glutamate, 10 parts of glycerine,
2 parts of potassium dihydrogen phosphate, 0.05 part of magnesium sulfate, 0.7 part of magnesium citrate, 15 parts, pH7.2 ± 0.2,121 DEG C sterilizings of potato starch
20min obtains egg medium, and abalone luminous bacillus is seeded to egg medium, cultivated at 32 DEG C of temperature, and it is maximum to obtain bacterium diameter
Bacterium colony is counted in parts by weight, takes 15 parts of peptone, 18 parts of beef extract, 20 parts of sodium chloride, 23 parts, pH7.2 ± 0.2 of agar, and 121
DEG C sterilizing 20min, obtain solid medium, by the bacterium colony streak inoculation of bacterium diameter maximum to solid medium culture 3 times, obtain bacterium colony,
It counts in parts by weight, takes 15 parts of peptone, 18 parts of beef extract, 20 parts, pH7.2 ± 0.2,121 DEG C sterilizing 20min of sodium chloride are obtained
Colony inoculation to fluid nutrient medium is increased bacterium and cultivates 40h for 23 DEG C in temperature, obtained enrichment culture medium, will increase bacterium training by fluid nutrient medium
Nutrient solution carries out centrifugation 20min with 7000r/min, breaks bacterium wall through cell squeezer, carries out centrifugation 20min again with 8000r/min,
Supernatant, in mass ratio 1：3, Na is added in into supernatant2P2O7Dialysis, obtain level-one dialysis enzyme solution, in mass ratio 1：6, Xiang Yi
Grade dialysis enzyme solution adds in polyethylene glycol dialysis, concentrates to get fluorescein.
A kind of preparation for being used to prevent oriental tobacco budworm, is counted in parts by weight, and the preparation for being used to prevent oriental tobacco budworm is by following
Into being grouped as：30 parts of water, 15 parts of wrappages, 15 parts of glucides, 8 parts of fragrance matters, 4 parts of plant extracts, 4 parts of fluoresceins.
A kind of preparation method for being used to prevent the preparation of oriental tobacco budworm as described in claim 1, which includes as follows
Step：
（1）Water, coating agent are subjected to Hybrid Heating, temperature rise is stirred 15min to 50 DEG C, and with 200r/min, must stir
Mix mixture；
（2）Glucide, fragrance matter, plant extracts, fluorescein are mixed, and are stirred with 200r/min, is stirred
Stirring mixture is added in during mixing, obtains mixture to get for preventing the preparation of oriental tobacco budworm.
Embodiment 4
Wrappage is beta-cyclodextrin.
Glucide is in mass ratio 1：3, sucrose, honey are mixed, obtain mixture to get glucide.
Plant extracts is that trifoliate jewelvine leaf is put into pulverizer to crush, and obtains crushed material, in mass ratio 1：4, it will crush
Object, 1.0mol/L ethanol solutions are mixed, and are divided into three parts, are impregnated 3,5,7 d, filtering respectively, and merge 3 filtrates, must be filtered
Filtrate in pressure 0.03MPa is concentrated under reduced pressure, obtains concentrate, concentrate is freeze-dried by liquid, collects dried object,
Up to plant extraction liquid.
A kind of preparation for being used to prevent oriental tobacco budworm, is counted in parts by weight, and the preparation for being used to prevent oriental tobacco budworm is by following
Into being grouped as：30 parts of water, 15 parts of wrappages, 15 parts of glucides, 4 parts of plant extracts.
A kind of preparation method for being used to prevent the preparation of oriental tobacco budworm as described in claim 1, which includes as follows
Step：
（1）Water, coating agent are subjected to Hybrid Heating, temperature rise is stirred 15min to 50 DEG C, and with 200r/min, must stir
Mix mixture；
（2）Glucide, plant extracts are mixed, and are stirred with 200r/min, stirring is added in whipping process
Mixture obtains mixture to get for preventing the preparation of oriental tobacco budworm.
Experiment：100 oriental tobacco budworms are taken, the medicament of the preparation of embodiment 4 of 50g after waiting for a period of time, finds to attract about 73
Oriental tobacco budworm.It follows that the medicament of the adding carbohydrate substance prepared by embodiment 4 has oriental tobacco budworm higher attraction.
Embodiment 5
Wrappage is beta-cyclodextrin.
Fragrance matter preparation method is that capsicum is put into stone mortar to smash to pieces, obtains chilli powder, counts in parts by weight, take 5 parts of water,
4 portions of edible oils, 2 portions of chilli powders, chilli powder, water are first mixed, and be stirred 15min with 150r/min, obtain mixture,
Edible oil is heated to 155 DEG C again, is directly added into mixture, is stirred when adding in 100r/min, after storage for 24 hours,
Filtering, obtains filter residue to get fragrance matter.
Plant extracts is that trifoliate jewelvine leaf is put into pulverizer to crush, and obtains crushed material, in mass ratio 1：4, it will crush
Object, 1.0mol/L ethanol solutions are mixed, and are divided into three parts, are impregnated 3,5,7 d, filtering respectively, and merge 3 filtrates, must be filtered
Filtrate in pressure 0.03MPa is concentrated under reduced pressure, obtains concentrate, concentrate is freeze-dried by liquid, collects dried object,
Up to plant extraction liquid.
A kind of preparation for being used to prevent oriental tobacco budworm, is counted in parts by weight, and the preparation for being used to prevent oriental tobacco budworm is by following
Into being grouped as：30 parts of water, 15 parts of wrappages, 8 parts of fragrance matters, 4 parts of plant extracts.
A kind of preparation method for being used to prevent the preparation of oriental tobacco budworm as described in claim 1, which includes as follows
Step：
（1）Water, coating agent are subjected to Hybrid Heating, temperature rise is stirred 15min to 50 DEG C, and with 200r/min, must stir
Mix mixture；
（2）Fragrance matter, plant extracts are mixed, and are stirred with 200r/min, stirring is added in whipping process
Mixture obtains mixture to get for preventing the preparation of oriental tobacco budworm.
Experiment：100 oriental tobacco budworms are taken, the medicament of the preparation of embodiment 5 of 50g after waiting for a period of time, finds to attract about 62
Oriental tobacco budworm.It follows that the medicament of the addition fragrance matter prepared by embodiment 5 has oriental tobacco budworm higher attraction.
Embodiment 6
Wrappage is beta-cyclodextrin.
Plant extracts is that trifoliate jewelvine leaf is put into pulverizer to crush, and obtains crushed material, in mass ratio 1：4, it will crush
Object, 1.0mol/L ethanol solutions are mixed, and are divided into three parts, are impregnated 3,5,7 d, filtering respectively, and merge 3 filtrates, must be filtered
Filtrate in pressure 0.03MPa is concentrated under reduced pressure, obtains concentrate, concentrate is freeze-dried by liquid, collects dried object,
Up to plant extraction liquid.
The preparation method of fluorescein：It counts in parts by weight, takes 80 parts of Fresh Egg liquid, 15 parts of sodium glutamate, 10 parts of glycerine,
2 parts of potassium dihydrogen phosphate, 0.05 part of magnesium sulfate, 0.7 part of magnesium citrate, 15 parts, pH7.2 ± 0.2,121 DEG C sterilizings of potato starch
20min obtains egg medium, and abalone luminous bacillus is seeded to egg medium, cultivated at 32 DEG C of temperature, and it is maximum to obtain bacterium diameter
Bacterium colony is counted in parts by weight, takes 15 parts of peptone, 18 parts of beef extract, 20 parts of sodium chloride, 23 parts, pH7.2 ± 0.2 of agar, and 121
DEG C sterilizing 20min, obtain solid medium, by the bacterium colony streak inoculation of bacterium diameter maximum to solid medium culture 3 times, obtain bacterium colony,
It counts in parts by weight, takes 15 parts of peptone, 18 parts of beef extract, 20 parts, pH7.2 ± 0.2,121 DEG C sterilizing 20min of sodium chloride are obtained
Colony inoculation to fluid nutrient medium is increased bacterium and cultivates 40h for 23 DEG C in temperature, obtained enrichment culture medium, will increase bacterium training by fluid nutrient medium
Nutrient solution carries out centrifugation 20min with 7000r/min, breaks bacterium wall through cell squeezer, carries out centrifugation 20min again with 8000r/min,
Supernatant, in mass ratio 1：3, Na is added in into supernatant2P2O7Dialysis, obtain level-one dialysis enzyme solution, in mass ratio 1：6, Xiang Yi
Grade dialysis enzyme solution adds in polyethylene glycol dialysis, concentrates to get fluorescein.
A kind of preparation for being used to prevent oriental tobacco budworm, is counted in parts by weight, and the preparation for being used to prevent oriental tobacco budworm is by following
Into being grouped as：30 parts of water, 15 parts of wrappages, 4 parts of plant extracts, 4 parts of fluoresceins.
A kind of preparation method for being used to prevent the preparation of oriental tobacco budworm as described in claim 1, which includes as follows
Step：
（1）Water, coating agent are subjected to Hybrid Heating, temperature rise is stirred 15min to 50 DEG C, and with 200r/min, must stir
Mix mixture；
（2）Plant extracts, fluorescein are mixed, and are stirred with 200r/min, it is mixed that stirring is added in whipping process
Object is closed, obtains mixture to get for preventing the preparation of oriental tobacco budworm.
Experiment：100 oriental tobacco budworms are taken, the medicament of the preparation of embodiment 6 of 50g after waiting for a period of time, finds to attract about 81
Oriental tobacco budworm.It follows that the medicament of the adding carbohydrate substance prepared by embodiment 6 has oriental tobacco budworm higher attraction.
Comparative example：The medicament of the improvement oriental tobacco budworm of Qingdao Chemical Engineering Technology Co., Ltd production
Method：One piece of 4 mu of big ground is selected, is classified as four groups, using as the medicament prepared by embodiment and comparative example.Detection
The control effect of interior oriental tobacco budworm for 24 hours after medication.
From the foregoing, it will be observed that it is good for preventing the effect of the preparation of oriental tobacco budworm prevention oriental tobacco budworm prepared by the present invention, and will not
It pollutes the environment, is a kind of safety and efficient oriental tobacco budworm control agent, is worthy to be popularized and uses.
Claims (7)
1. a kind of preparation for being used to prevent oriental tobacco budworm, which is characterized in that count in parts by weight, the system for being used to prevent oriental tobacco budworm
Agent consists of the following compositions：20 ~ 30 parts of water, 12 ~ 15 parts of wrappages, 10 ~ 15 parts of glucides, 7 ~ 8 parts of fragrance matters, 3 ~ 4 parts
Plant extracts, 3 ~ 4 parts of fluoresceins.
2. it is used to prevent the preparation of oriental tobacco budworm according to claim 1, which is characterized in that the wrappage is beta-cyclodextrin.
3. it is used to prevent the preparation of oriental tobacco budworm according to claim 1, which is characterized in that the glucide is in mass ratio
1：2 ~ 3, sucrose, honey are mixed, obtain mixture to get glucide.
4. according to claim 1 for preventing the preparation of oriental tobacco budworm, which is characterized in that the fragrance matter preparation method is
Capsicum is put into stone mortar and is smashed to pieces, chilli powder is obtained, counts in parts by weight, takes 3 ~ 5 parts of water, 2 ~ 4 portions of edible oils, 1 ~ 2 portion of chilli powder,
First chilli powder, water are mixed, and 10 ~ 15min is stirred with 100 ~ 150r/min, obtains mixture, then edible oil is added
Heat is directly added into 155 DEG C in mixture, is stirred when adding in 80 ~ 100r/min, and after storage for 24 hours, filtering must filter
Slag is to get fragrance matter.
5. it is used to prevent the preparation of oriental tobacco budworm according to claim 1, which is characterized in that the plant extracts is by trifoliate jewelvine
Leaf is put into pulverizer and crushes, and obtains crushed material, in mass ratio 1：3 ~ 4, crushed material, 1.0mol/L ethanol solutions are mixed
Close, be divided into three parts, respectively impregnate 3,5,7 d, filtering, and merge 3 filtrates, obtain filtrate, by filtrate pressure 0.01 ~
0.03MPa is concentrated under reduced pressure, and obtains concentrate, and concentrate is freeze-dried, and collects dried object to get plant extraction liquid.
6. it is used to prevent the preparation of oriental tobacco budworm according to claim 1, which is characterized in that the preparation method of the fluorescein：
It counts in parts by weight, takes 50 ~ 80 parts of Fresh Egg liquid, 13 ~ 15 parts of sodium glutamate, 8 ~ 10 parts of glycerine, 1 ~ 2 part of potassium dihydrogen phosphate,
0.02 ~ 0.05 part of magnesium sulfate, 0.2 ~ 0.7 part of magnesium citrate, 12 ~ 15 parts, pH7.2 ± 0.2,121 DEG C sterilizings of potato starch
20min obtains egg medium, and abalone luminous bacillus is seeded to egg medium, is cultivated at 28 ~ 32 DEG C of temperature, obtains bacterium diameter most
Big bacterium colony, is counted in parts by weight, takes 10 ~ 15 parts of peptone, 15 ~ 18 parts of beef extract, 15 ~ 20 parts of sodium chloride, agar 20 ~ 23
Part, pH7.2 ± 0.2,121 DEG C of sterilizing 20min obtain solid medium, by the bacterium colony streak inoculation of bacterium diameter maximum to solid culture
Base culture 2 ~ 3 times, obtains bacterium colony, counts in parts by weight, takes 10 ~ 15 parts of peptone, 15 ~ 18 parts of beef extract, 15 ~ 20 parts of sodium chloride,
PH7.2 ± 0.2,121 DEG C of sterilizing 20min, obtains fluid nutrient medium, and colony inoculation to fluid nutrient medium is increased bacterium in temperature 20 ~ 23
DEG C culture 30 ~ 40h, obtain enrichment culture medium, enrichment culture medium be subjected to centrifugation 20min with 7000r/min, is broken through cell squeezer
Bacterium wall carries out centrifugation 20min with 8000r/min again, obtains supernatant, in mass ratio 1：3, Na is added in into supernatant2P2O7Thoroughly
Analysis, obtain level-one dialysis enzyme solution, in mass ratio 1：6, polyethylene glycol dialysis is added in level-one dialysis enzyme solution, is concentrated to get fluorescein.
A kind of 7. preparation method for being used to prevent the preparation of oriental tobacco budworm as described in claim 1, which is characterized in that the preparation method
Include the following steps：
（1）Water, coating agent are subjected to Hybrid Heating, temperature rise to 45 ~ 50 DEG C, and be stirred 10 with 150 ~ 200r/min ~
15min obtains stirring mixture；
（2）Glucide, fragrance matter, plant extracts, fluorescein are mixed, and stirred with 150 ~ 200r/min
It mixes, stirring mixture is added in whipping process, obtain mixture to get for preventing the preparation of oriental tobacco budworm.
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