CN105724365A - Black soldier fly egg refrigeration preservation method - Google Patents
Black soldier fly egg refrigeration preservation method Download PDFInfo
- Publication number
- CN105724365A CN105724365A CN201610109328.6A CN201610109328A CN105724365A CN 105724365 A CN105724365 A CN 105724365A CN 201610109328 A CN201610109328 A CN 201610109328A CN 105724365 A CN105724365 A CN 105724365A
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- Prior art keywords
- black soldier
- soldier flies
- ovum
- soldier fly
- eggs
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- 238000000034 method Methods 0.000 title claims abstract description 20
- 238000004321 preservation Methods 0.000 title claims abstract description 16
- 238000005057 refrigeration Methods 0.000 title abstract description 3
- 241000709785 Hermetia illucens Species 0.000 title abstract 5
- 241001481656 Stratiomyidae Species 0.000 claims abstract description 53
- 102000002322 Egg Proteins Human genes 0.000 claims description 41
- 108010000912 Egg Proteins Proteins 0.000 claims description 41
- 210000004681 ovum Anatomy 0.000 claims description 41
- 235000013601 eggs Nutrition 0.000 abstract description 20
- 230000012447 hatching Effects 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 238000009395 breeding Methods 0.000 abstract 1
- 230000001488 breeding effect Effects 0.000 abstract 1
- 230000002035 prolonged effect Effects 0.000 abstract 1
- 239000005416 organic matter Substances 0.000 description 12
- 235000013305 food Nutrition 0.000 description 9
- 235000015001 Cucumis melo var inodorus Nutrition 0.000 description 6
- 240000002495 Cucumis melo var. inodorus Species 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 6
- 241000382353 Pupa Species 0.000 description 6
- 230000032669 eclosion Effects 0.000 description 6
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 6
- 238000011534 incubation Methods 0.000 description 6
- 230000013011 mating Effects 0.000 description 6
- 230000017448 oviposition Effects 0.000 description 6
- 230000002411 adverse Effects 0.000 description 3
- 238000009826 distribution Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 210000003608 fece Anatomy 0.000 description 3
- 230000000384 rearing effect Effects 0.000 description 3
- 230000007115 recruitment Effects 0.000 description 3
- 239000002917 insecticide Substances 0.000 description 2
- 239000010871 livestock manure Substances 0.000 description 2
- AILDTIZEPVHXBF-UHFFFAOYSA-N Argentine Natural products C1C(C2)C3=CC=CC(=O)N3CC1CN2C(=O)N1CC(C=2N(C(=O)C=CC=2)C2)CC2C1 AILDTIZEPVHXBF-UHFFFAOYSA-N 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 244000308495 Potentilla anserina Species 0.000 description 1
- 235000016594 Potentilla anserina Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
- 230000009571 larval growth Effects 0.000 description 1
- 230000029052 metamorphosis Effects 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0278—Physical preservation processes
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Animal Behavior & Ethology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a black soldier fly egg refrigeration preservation method. The method comprises the following steps: tiling black soldier fly eggs in a container, and refrigerating the eggs at the temperature of 0-10 DEG C under the relative humidity of 70-80 percent. According to the method disclosed by the invention, the preservation time of the black soldier fly eggs can be effectively prolonged, and a hatching rate of the eggs can be kept well. Therefore, the black soldier fly breeding efficiency can be improved, less labor is used, the total number of lots of fed black soldier flies is increased, and the method is very good for actual production.
Description
Technical field:
The invention belongs to black soldier flies and raise field, be specifically related to a kind of black soldier flies ovum stored refrigerated method.
Background technology:
Black soldier flies originates from the savanna of South America, mainly with the feces of grassland animal and corpse for food, is gradually diffused into whole American continent subsequently, to the middle of last century, has the distribution of black soldier flies to the Seattle of the U.S. from Argentine southernmost end.During the Second World War, spreading of war creates substantial amounts of corpse, and black soldier flies rapidly diffuses into the whole world along with U.S. army, has distribution in the most area in the torrid zone in the whole world, subtropical zone and temperate zone at present.Black soldier flies has distribution to record in areas such as the Guangdong of China, Guangxi, Hainan, Yunnan, Sichuan, Fujian, Hebei, Beijing, belongs to the Common Species of stratiomyiid.
Black soldier flies is a kind of insecticide of Diptera Stratiomyidae, and adult is similar to fly but individual larger.Larval feeding scope widely, is the important step in nature detrital food chain.Black soldier flies is commonly near swinery, hen house, takes food fresh pig manure, chicken manure, and larva, while converting garbage into into insecticide biological chain, is effectively reduced pollution, is a kind of desirably scavenger insect.
Heisui River Gadfly complete metamorphosis, all one's life to experience ovum, larva, pupa, adult four-stage.Adult has Flower-visiting habit, with the juice of plant and honeydew for food, generally with have brushwood greenery patches for habitat, female adult worm finds fresh organic matter as place of laying eggs, and is produced in the dry gap near organic matter by ovum.After adult eclosion can mating, start to lay eggs after about 2～3d, single female egg laying amount about 900, poly-produce.Ovum oblong, the initial stage is translucent, after fade to faint yellow, egg hatch need 2～6d.
The preservation of worm's ovum makes fed-batch be possibly realized, the length of holding time determine raising batch, and batch many at least have influence on raise total amount sizes.To raise the calculating of a collection of worm every 6 days, if storage life extends to 25 days, then raising batch can bring up to 4 times.Therefore, finding a kind of high-efficient simple store method, to improving black soldier flies feeding efficiency, reduce recruitment, the total number improving black soldier flies mass rearing has great importance；Meanwhile, also can avoid the adverse effect that the factors such as vile weather are brought to cultivation, to reach the predetermined purpose produced with conservation.
Summary of the invention:
It is an object of the invention to provide a kind of black soldier flies ovum stored refrigerated method of high-efficient simple, this method, to improving black soldier flies feeding efficiency, reduces recruitment, and the total number improving black soldier flies mass rearing has great importance；Meanwhile, also can avoid the adverse effect that the factors such as vile weather are brought to cultivation, to reach the predetermined purpose produced with conservation.
The black soldier flies ovum stored refrigerated method of the present invention, it is characterised in that be laid in container by black soldier flies ovum, is subsequently placed in temperature 0 DEG C～10 DEG C, relative humidity is 70%～80% time cold preservation.
Described tiling, the thickness of the black soldier flies ovum of its tiling is preferably 0.5cm.
Described container is preferably culture dish or pallet.
The black soldier flies ovum stored refrigerated method of the present invention, compared to prior art, has the advantages that
Utilizing the black soldier flies ovum stored refrigerated method of the present invention, its holding time is long, can meet the demand in batches hatched in producing, to reach the purpose carrying out producing according to plan.Normal process without preservation and the incubation rate of black soldier flies ovum directly hatched is about 70%, and after utilizing the holding time of black soldier flies ovum that the method for the present invention preserves to extend to 25 days, incubation rate also can reach more than 30%.Adopt the ovum after the inventive method cold preservation, relatively slightly postpone without the egg hatching time of cold preservation, but the brooding time taken out on the same day is basically identical, namely after the black soldier flies ovum cold preservation of not same date, take out on the same day and can hatch through 4～7 days.This is very meaningful aborning so that every batch of larval growth raised is consistent, and regularity is high.The length of holding time determine raising batch, batch many at least have influence on raise total amount sizes.Such as, to hatch a collection of calculating every 6 days, cold preservation 25 days, raising batch can increase to 4 batches, and the corresponding total amount producing black soldier flies larva also will increase to 4 times.It addition, technique of refrigeration makes conservation be possibly realized, can effectively control black soldier flies egg hatching speed, avoid the adverse effect that vile weather brings to cultivation.In sum, the present invention can improve black soldier flies cultivation efficiency, less recruitment, improves the total number of black soldier flies mass rearing, and actual production is highly beneficial.
Detailed description of the invention:
Following example are further illustrating the present invention, rather than limitation of the present invention.
Embodiment 1:
Black soldier flies pupa is placed in adult cultivation greenhouse, and the adult after emergence has Flower-visiting habit, with the juice of plant and honeydew for food, with have brushwood greenery patches for habitat.After adult eclosion can mating, female adult worm finds fresh organic matter as place of laying eggs, and is produced in the dry gap near organic matter by ovum after about 2～3d.It is afternoon that egg-laying time is generally concentrated, and continues about half an hour, collects the black soldier flies ovum produced the same day after laying eggs and terminating, evenly laid out in the culture dish that diameter is 8cm, keeps the weight of ovum in each culture dish consistent, and tiling thickness is about 0.5cm.By fill black soldier flies ovum culture dish move into temperature be 0 DEG C, relative humidity be 70% freezer in carry out cold preservation, taking-ups in 25 days is hatched, after the hatching of 4～7 days acquisition black soldier flies larva, incubation rate is 28%.
Embodiment 2:
Black soldier flies pupa is placed in adult cultivation greenhouse, and the adult after emergence has Flower-visiting habit, with the juice of plant and honeydew for food, with have brushwood greenery patches for habitat.After adult eclosion can mating, female adult worm finds fresh organic matter as place of laying eggs, and is produced in the dry gap near organic matter by ovum after about 2～3d.It is afternoon that egg-laying time is generally concentrated, and continues about half an hour, collects the black soldier flies ovum produced the same day after laying eggs and terminating, evenly laid out in the culture dish that diameter is 8cm, keeps the weight of ovum in each culture dish consistent, and tiling thickness is about 0.5cm.By fill black soldier flies ovum culture dish move into temperature be 0 DEG C, relative humidity be 70% freezer in carry out cold preservation, taking-ups in 15 days is hatched, after the hatching of 4～7 days acquisition black soldier flies larva, incubation rate is 40%.
Embodiment 3:
Black soldier flies pupa is placed in adult cultivation greenhouse, and the adult after emergence has Flower-visiting habit, with the juice of plant and honeydew for food, with have brushwood greenery patches for habitat.After adult eclosion can mating, female adult worm finds fresh organic matter as place of laying eggs, and is produced in the dry gap near organic matter by ovum after about 2～3d.It is afternoon that egg-laying time is generally concentrated, and continues about half an hour, collects the black soldier flies ovum produced the same day after laying eggs and terminating, evenly laid out in the culture dish that diameter is 8cm, keeps the weight of ovum in each culture dish consistent, and tiling thickness is about 0.5cm.By fill black soldier flies ovum culture dish move into temperature be 10 DEG C, relative humidity be 80% freezer in carry out cold preservation, taking-ups in 25 days is hatched, after the hatching of 4～7 days acquisition black soldier flies larva, incubation rate is 30%.
Embodiment 4:
Black soldier flies pupa is placed in adult cultivation greenhouse, and the adult after emergence has Flower-visiting habit, with the juice of plant and honeydew for food, with have brushwood greenery patches for habitat.After adult eclosion can mating, female adult worm finds fresh organic matter as place of laying eggs, and is produced in the dry gap near organic matter by ovum after about 2～3d.It is afternoon that egg-laying time is generally concentrated, and continues about half an hour, collects the black soldier flies ovum produced the same day after laying eggs and terminating, evenly laid out in the culture dish that diameter is 8cm, keeps the weight of ovum in each culture dish consistent, and tiling thickness is about 0.5cm.By fill black soldier flies ovum culture dish move into temperature be 5 DEG C, relative humidity be 75% freezer in carry out cold preservation, taking-ups in 25 days is hatched, after the hatching of 4～7 days acquisition black soldier flies larva, incubation rate is 43%.
Comparative example:
Black soldier flies pupa is placed in adult cultivation greenhouse, and the adult after emergence has Flower-visiting habit, with the juice of plant and honeydew for food, with have brushwood greenery patches for habitat.After adult eclosion can mating, female adult worm finds fresh organic matter as place of laying eggs, and is produced in the dry gap near organic matter by ovum after about 2～3d.It is afternoon that egg-laying time is generally concentrated, continue about half an hour, the black soldier flies ovum produced the same day is collected after laying eggs and terminating, evenly laid out in the culture dish that diameter is 8cm, keep the weight of ovum in each culture dish consistent, tiling thickness is about 0.5cm, it is subsequently placed in room temperature 25 DEG C, through 3-7 days, the black soldier flies larva of about 70% will hatch out gradually, it is impossible to the production time according to expectation hatches, and is unfavorable for the preservation of black soldier flies ovum.
Claims (3)
1. a black soldier flies ovum stored refrigerated method, it is characterised in that be laid in container by black soldier flies ovum, is subsequently placed in temperature 0 DEG C～10 DEG C, relative humidity is 70%～80% time cold preservation.
2. black soldier flies ovum stored refrigerated method according to claim 1, it is characterised in that described tiling, the thickness of the black soldier flies ovum of its tiling is 0.5cm.
3. black soldier flies ovum stored refrigerated method according to claim 1, it is characterised in that described container is culture dish or pallet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610109328.6A CN105724365A (en) | 2016-02-26 | 2016-02-26 | Black soldier fly egg refrigeration preservation method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610109328.6A CN105724365A (en) | 2016-02-26 | 2016-02-26 | Black soldier fly egg refrigeration preservation method |
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| Publication Number | Publication Date |
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| CN105724365A true CN105724365A (en) | 2016-07-06 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610109328.6A Pending CN105724365A (en) | 2016-02-26 | 2016-02-26 | Black soldier fly egg refrigeration preservation method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112493233A (en) * | 2020-12-23 | 2021-03-16 | 扬州大学 | Cold storage method of bean hawkmoth eggs |
| WO2021145760A1 (en) * | 2020-01-17 | 2021-07-22 | Protix B.V. | Method for controlling hatching of black soldier fly eggs |
| EP4072286A4 (en) * | 2019-12-10 | 2024-01-17 | Yeda Res & Dev | Cryopreserved insects and methods for producing same |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613169A (en) * | 2012-04-26 | 2012-08-01 | 南京农业大学 | Method for prolonging preservation period of rice leafroller eggs |
| CN104823927A (en) * | 2015-06-04 | 2015-08-12 | 云南农业大学 | Method for preserving plusia agnata staudinger eggs |
-
2016
- 2016-02-26 CN CN201610109328.6A patent/CN105724365A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613169A (en) * | 2012-04-26 | 2012-08-01 | 南京农业大学 | Method for prolonging preservation period of rice leafroller eggs |
| CN104823927A (en) * | 2015-06-04 | 2015-08-12 | 云南农业大学 | Method for preserving plusia agnata staudinger eggs |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4072286A4 (en) * | 2019-12-10 | 2024-01-17 | Yeda Res & Dev | Cryopreserved insects and methods for producing same |
| WO2021145760A1 (en) * | 2020-01-17 | 2021-07-22 | Protix B.V. | Method for controlling hatching of black soldier fly eggs |
| CN112493233A (en) * | 2020-12-23 | 2021-03-16 | 扬州大学 | Cold storage method of bean hawkmoth eggs |
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Application publication date: 20160706 |
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