CN112493233A

CN112493233A - Cold storage method of bean hawkmoth eggs

Info

Publication number: CN112493233A
Application number: CN202011540644.1A
Authority: CN
Inventors: 陆明星; 朱晨旭; 苏颖; 徐伟; 刘兴昌; 杜予州
Original assignee: Yangzhou University
Current assignee: Yangzhou University
Priority date: 2020-12-23
Filing date: 2020-12-23
Publication date: 2021-03-16

Abstract

The invention discloses a cold storage method of a bean hawkmoth egg, which comprises the following steps: (1) placing the bean hawkmoth eggs in a culture dish, and placing wetting filter paper at the bottom of the culture dish; (2) the culture dish is placed in an incubator, stored for 1 day at 20 ℃ with humidity of 70 +/-10% under dark conditions, and then transposed for storage at 4-20 ℃. The refrigeration method can effectively delay the incubation time of the bean hawkmoth eggs and prolong the storage time of the bean hawkmoth eggs, the incubation time of the eggs can be delayed by more than 14 days, the incubation rate of the eggs can reach 90 percent at most, the cost is low, the refrigeration method has important significance for various biological characteristic researches and large-scale production of the bean hawkmoth, and provides important guarantee for artificial feeding of the bean hawkmoth.

Description

Cold storage method of bean hawkmoth eggs

Technical Field

The invention belongs to the field of large-scale production research of edible insects, and particularly relates to a cold storage method of a bean hawkmoth egg.

Background

Clanis bilineata tsingtauica Mel (Clanis bilineata Mel) belongs to Lepidoptera (Lepidotera), Sphingideae, Clanis of Leguminosae, also called Clanis bilineata, and is a common leaf-eating insect in leguminous plants. The bean hawkmoth is grown for 1-2 generations in China in one year, grown for one generation in Jiangsu in one year, and has 5 instars of larvae and backlight property. 1-2 instar larva takes a feeding leaf to form a hole or bites the edge of the feeding leaf to form an indentation; increasing the food intake of 3-4 instar larvae; when the 5 th year reaches the overeating period, the whole soybean can be eaten into light stalks. The old larvae successively fall to the ground in the last 9 th month, and overwinter in the soil with the depth of 9-12 cm such as soil heaps, ridges and the like near the bean field or leguminous plants. The bean hawkmoth is deeply liked by people in Jiangsu, Shandong, Henan and the like as an edible insect and is widely popularized as a special delicious dish. At present, the market of the bean hawkmoth products is gradually expanded, and the industry development prospect is optimistic. However, the mass breeding of the bean hawkmoth depends on the planting of the soybeans, so the growth condition and the scale of the soybeans severely restrict the breeding scale and the breeding speed of the soybeans. Although the greenhouse artificial feeding scale of the bean hawkmoth is gradually enlarged at present, the bean hawkmoth can be fed for three generations in Jiangsu year under an ideal state, but no proper soybean is usually provided when the bean hawkmoth eggs are put in the artificial propagation process, or the quality of the bean hawkmoth eggs is poor. Therefore, many of the soybean hornworm eggs hatch while waiting for release, and the soybean hornworm hatched at first time cannot eat the larvae, so that the larvae die, and huge loss is caused. And the egg period of the bean hawkmoth is short, generally 3-5 days, and a lot of larvae are hatched to cause loss particularly in the long-distance transportation process.

Disclosure of Invention

The purpose of the invention is as follows: the technical problem to be solved is to provide a cold storage method for the soybean hornworm eggs, the method can effectively prolong the storage time of the soybean hornworm eggs, and has no influence on the subsequent larva incubation.

The technical scheme is as follows: in order to solve the technical problems, the invention provides the following technical scheme:

a cold storage method for the eggs of the bean hawkmoth comprises the following steps:

(1) placing the soybean hornworm eggs in a culture dish, wherein the placing amount of the soybean hornworm eggs is proper and is not excessive so as to prevent the self-mutilation phenomenon caused by the fact that a large number of larvae hatch out at the same time, and placing wetting filter paper at the bottom of the culture dish;

(2) placing the culture dish in an incubator, storing for 1 day at 20 ℃ and humidity of 70 +/-10% under dark conditions, and then transposing for storing at 4-20 ℃, wherein when discovering that the soybean hornworm larvae need to be taken out in time in the storage process, other stored eggs are prevented from being damaged. The method can remarkably prolong the hatching time, and has high hatching rate,

in the step (1), the soybean hornworm eggs are soybean hornworm eggs laid by 1-3 days old, and the soybean hornworm eggs laid by 2 days old are preferred.

In step (2), the filter paper is kept in a wet state when stored at 4-20 ℃.

In the step (2), the environmental humidity is 70 +/-10% when the food is stored at the temperature of 4-20 ℃.

In the step (2), the preservation time is 10-20 days at 4-20 ℃.

If the field temperature is too high, the larvae are not required to be immediately inoculated into the field after hatching, and the larvae are required to be placed indoors for 1-2 hours at the temperature of 28 ℃.

Has the advantages that:

the invention discloses a cold storage method for bean hawkmoth eggs, which can effectively delay the incubation time of the bean hawkmoth eggs and prolong the storage time, the incubation time of the eggs can be delayed for more than 14 days, the incubation rate of the eggs can reach 90 percent at most, the cost is lower, the cold storage method has important significance for various biological characteristic researches and large-scale production of the bean hawkmoth, and provides important guarantee for artificial feeding of the bean hawkmoth.

Drawings

FIG. 1 is a schematic diagram of a culture dish on which a bean hawkmoth egg is placed, and FIG. 1 is a filter paper.

FIG. 2 comparative analysis of the hatchability of the bean hawkmoth eggs from different treatments.

FIG. 3 comparison of the storage time of different treated Ottelia suzukii eggs.

(a, b, c, d, e in FIGS. 2 and 3 represent significant differences between the different treatments.)

Detailed Description

The invention will be better understood from the following examples. However, those skilled in the art will readily appreciate that the description of the embodiments is only for illustrating the present invention and should not be taken as limiting the invention as detailed in the claims.

The specific implementation steps of the cold storage and preservation technology of the bean hawkmoth eggs are divided into three steps, and the specific implementation steps are as follows:

(1) selecting effective eggs (about 2 days old) of the adult soybean hawkmoth mating and laying eggs, placing a piece of wet filter paper at the bottom of the culture dish to ensure the humidity (as shown in figure 1), and placing the culture dish in an incubator at 20 ℃ for 1 day.

(2) Taking out the culture dish containing the bean hawkmoth eggs on the next day, respectively placing the culture dishes in culture boxes with different temperatures, and simultaneously paying attention to the humidity of filter paper in the culture dish, and timely supplementing proper water without causing water accumulation. Eggs were observed daily for hatching, death and recording. Meanwhile, eggs always placed at 28 ℃ were used as a control.

(3) When the eggs in the culture dish are hatched, the eggs are immediately inoculated to soybean leaves respectively for feeding, and the growth condition is observed, the temperature of the culture box is set to be 28 +/-1 ℃, the humidity is 60%, and the photoperiod L: D is 16: 8.

The temperature setting for the refrigeration process of the present invention is based on the previous experimental data. In order to search the temperature and time which are most suitable for refrigerating the asparagus caterpillars, the incubation time and the incubation rate of eggs under different temperature-changing treatments are searched in the process of the invention.

The experiment was run with a total of 19 treatments (including controls):

treatment 1: standing at 20 deg.C for 6 days, standing at 4 deg.C for 7 days, and placing in 28 deg.C incubator;

and (3) treatment 2: standing at 20 deg.C for 6 days, standing at 12 deg.C for 7 days, and placing in 28 deg.C incubator;

and (3) treatment: standing at 20 deg.C for 13 days, and placing in 28 deg.C incubator;

and (4) treatment: standing at 20 deg.C for 6 days, standing at 4 deg.C for 7 days, and placing in 4-20 deg.C incubator;

and (4) treatment 5: standing at 20 deg.C for 6 days, standing at 12 deg.C for 7 days, and placing in 4-20 deg.C incubator;

and (6) treatment: standing at 20 deg.C for 13 days, and placing in 4-20 deg.C incubator;

and (7) treatment: standing at 20 deg.C for 11 days, standing at 4 deg.C for 7 days, and placing in 28 deg.C incubator;

and (4) treatment 8: standing at 20 deg.C for 11 days, standing at 12 deg.C for 7 days, and placing in 28 deg.C incubator;

and (4) treatment: standing at 20 deg.C for 18 days, and placing in 28 deg.C incubator;

and (4) treatment 10: standing at 20 deg.C for 11 days, standing at 4 deg.C for 7 days, and placing in 4-20 deg.C incubator; ,

and (3) treatment 11: standing at 20 deg.C for 11 days, standing at 12 deg.C for 7 days, and placing in 4-20 deg.C incubator;

and (4) treatment 12: standing at 20 deg.C for 18 days, and placing in 4-20 deg.C incubator;

and (4) treatment: standing at 20 deg.C for 1 day, and placing in 4 deg.C incubator;

and (4) processing: standing at 20 deg.C for 1 day, and placing in 12 deg.C incubator;

and (4) treatment: placing in an incubator at 20 deg.C;

and (3) treatment 16: standing at 20 deg.C for 1 day, standing at 4 deg.C for 11 days, and placing in 4-20 deg.C incubator;

and (6) treatment 17: standing at 20 deg.C for 1 day, standing at 12 deg.C for 11 days, and placing in 4-20 deg.C incubator;

and (4) treatment: standing at 20 deg.C for 12 days, and placing in 4-20 deg.C incubator;

comparison: placing in an incubator at 28 ℃. 30 identical day-old and uniformly colored Oncomelania pisifera eggs were placed in each dish, and each treatment was repeated 3 times.

The research of the test results shows that: the hatchability of treatments 13-18 was higher and not significantly different from the control group (table 1, table 4 and figure 2). Hatchability obtained from the remaining groups of treatments differed significantly from the control group, with

treatments

4 and 5 being the lowest hatchability. In terms of the storage time, the eggs of treatment 4 had the longest storage time, and the eggs of treatment 18 had the shortest storage time (tables 2 to 3).

The reason for finally selecting the eggs to be treated at 4-20 ℃ is as follows: (1) under refrigeration in treatments 13-18, the hatchability of the eggs was high, up to 90%. The difference from the control group is not significant. (2) The treatment 13-18 can delay the incubation of the eggs for 13-20 days, and basically meets the aim of prolonging the preservation time of the eggs in the process of scale propagation of the bean hawkmoth (tables 2-3 and figures 2-3). However, the mortality rate for the treatment of 13-15 g of the soybean hornworm eggs was higher than that for the treatment of 16-18 g (Table 4). Therefore, the low-temperature treatment technology for the soybean hornworm eggs finally selected in the patent is 20 ℃, the soybean hornworm eggs are placed for 1 day and are transposed to be stored at the temperature of 4-20 ℃.

TABLE 1 control group hatchability

TABLE 2 incubation dates and storage times of eggs in treatment groups

TABLE 3 preservation time (days) for different treated eggs

TABLE 4 incubation of different treated eggs

Claims

1. A cold storage method for a bean hawkmoth egg is characterized by comprising the following steps:

(1) placing the bean hawkmoth eggs in a culture dish, and placing wetting filter paper at the bottom of the culture dish;

(2) the culture dish is placed in an incubator, stored for 1 day at 20 ℃ with humidity of 70 +/-10% under dark conditions, and then transposed for storage at 4-20 ℃.

2. The method for cold preservation of the bean hawkmoth eggs according to claim 1, wherein in the step (1), the bean hawkmoth eggs are the bean hawkmoth eggs laid for 1-3 days old.

3. The method for cold preservation of a soybean hornworm egg according to claim 1, wherein in the step (2), the filter paper is kept in a wet state during preservation at 4 to 20 ℃.

4. The method for cold preservation of a soybean hornworm egg according to claim 1, wherein in the step (2), the ambient humidity is 70 ± 10% when the soybean hornworm egg is preserved at 4 to 20 ℃.

5. The method for cold preservation of a bean hawkmoth egg according to claim 1, wherein in the step (2), the egg is preserved at 4-20 ℃ for 10-20 days.

6. The method for cold preservation of the eggs of the bean hawkmoth as claimed in claim 1, wherein in the step (2), the incubator is an intelligent climatic chamber with model number of RXZ-280A.