CN101326902B - Method for inducing diapause of Trichogramma ostriniae by constant temperature and storage method - Google Patents
Method for inducing diapause of Trichogramma ostriniae by constant temperature and storage method Download PDFInfo
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- CN101326902B CN101326902B CN200810055331XA CN200810055331A CN101326902B CN 101326902 B CN101326902 B CN 101326902B CN 200810055331X A CN200810055331X A CN 200810055331XA CN 200810055331 A CN200810055331 A CN 200810055331A CN 101326902 B CN101326902 B CN 101326902B
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- temperature
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- diapause
- oophagous trichogrammae
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- 238000003860 storage Methods 0.000 title claims abstract description 33
- 230000005058 diapause Effects 0.000 title claims abstract description 27
- 230000001939 inductive effect Effects 0.000 title claims abstract description 17
- 238000000034 method Methods 0.000 title claims abstract description 16
- 241000406562 Trichogramma ostriniae Species 0.000 title claims description 15
- 210000004681 ovum Anatomy 0.000 claims abstract description 18
- 238000004519 manufacturing process Methods 0.000 claims abstract description 14
- 241000256618 Trichogramma Species 0.000 claims abstract description 10
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 5
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 5
- 241000256844 Apis mellifera Species 0.000 claims description 31
- 241000346285 Ostrinia furnacalis Species 0.000 claims description 25
- 238000005057 refrigeration Methods 0.000 claims description 12
- 238000005286 illumination Methods 0.000 claims description 5
- 230000006698 induction Effects 0.000 claims description 3
- 238000011084 recovery Methods 0.000 claims description 3
- 239000012467 final product Substances 0.000 claims description 2
- 230000007774 longterm Effects 0.000 abstract description 9
- 238000009395 breeding Methods 0.000 abstract description 5
- 230000001488 breeding effect Effects 0.000 abstract description 5
- 238000005516 engineering process Methods 0.000 abstract description 5
- 241000607479 Yersinia pestis Species 0.000 abstract description 4
- 230000016853 telophase Effects 0.000 abstract 1
- 230000003071 parasitic effect Effects 0.000 description 7
- 241000753145 Sitotroga cerealella Species 0.000 description 3
- 240000008042 Zea mays Species 0.000 description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 3
- 241000238631 Hexapoda Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 239000004744 fabric Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000011031 large-scale manufacturing process Methods 0.000 description 2
- 230000017448 oviposition Effects 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 241000256837 Apidae Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 230000032669 eclosion Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 210000004894 snout Anatomy 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
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Abstract
The invention provides a constant-temperature inducing maize-borer oophagous trichogrammae diapause storage method. The collected maize-borer oophagous trichogrammaes which have developed to the ovum telophase from the middle larva stage adopt constant-temperature inducing maize-borer oophagous trichogrammae diapause, and are stored under a specific temperature and humidity, and the development of the maize-borer oophagous trichogrammae is restored by changing the prior temperature to use. The method is applicable to oophagous trichogrammae breeding by using small gelechiid ovum; the technology matches large-scale oophagous trichogrammae production; the method is applicable to long-term production and storage of maize-borer oophagous trichogrammaes; thereby, sufficient maize-borer oophagous trichogrammaes are prepared for pest control, and the method can also be used to store breeding maize-borer oophagous trichogrammaes.
Description
Technical field
The present invention relates to the diapause storage method of insect, particularly the long term storage method of the advantage of foodstuff pest corn borer parasite-corn borer oophagous trichogrammae.
Background technology
Corn borer oophagous trichogrammae (Trichogramma ostriniae Pang et Chen) is the advantage natural enemy of the most of regional parasitic corn primary pest corn borer of China, low-cost, this honeybee kind of large-scale production and be used for the corn borer control effectively Pest Control cause harm raising China grain yield.At present, China has successfully realized raising the corn borer oophagous trichogrammae with gelechiid ova or rice moth egg, in view of the oophagous trichogrammae storage stability of routine breeding poor, utilize the characteristic of anti-storage after the oophagous trichogrammae diapause, inducing diapause of Trichogramma ostriniae also realizes that long term storage is to prolong the oophagous trichogrammae effective production time, expand the scale of production one of optimal path that reduces production costs.At present, the existing result of study with rice moth egg breeding corn borer oophagous trichogrammae of China shows, by alternating temperature induce (respectively 20～25 ℃ with 10～15 ℃ of two temperature sections in select preference temperature, alternating temperature is handled.With it Dui Ying constant temperature induce for: in a temperature section, select preference temperature, single Temperature Treatment.) can make the corn borer oophagous trichogrammae of parasitic rice moth egg enter diapause.Also do not induce the diapause of Trichogramma ostriniae storing technology of parasitic gelechiid ova in the prior art.
Summary of the invention
The objective of the invention is to solve above difficulty, the method for a kind of long term storage with the corn borer oophagous trichogrammae of gelechiid ova large-scale production is provided.The corn borer oophagous trichogrammae is the important natural enemy of parasitic corn borer, is the advantage natural enemy of the most of regional corn field of China, by artificial release and protection utilization corn borer is had the better controlled effect.At present, propagation technique with parasitic granule ovum oophagous trichogrammaes such as gelechiid ova factory farming corn borer oophagous trichogrammaes is comparatively ripe, can not long term storage cause production scale little for solving oophagous trichogrammae, a series of problems such as production cost height have very urgent requirement to the long term storage of this honeybee kind.By experiment more than 3 years and production practices, formed the inducing diapause of Trichogramma ostriniae by constant temperature storing technology that a cover can be combined closely with the gelechiid parasitic bee, use this technology oophagous trichogrammae production and can shift to an earlier date more than 6 months, the oophagous trichogrammae eclosion rate after the storage is still up to more than 90%.
Purpose of the present invention realizes by following measure:
1, inducing diapause of Trichogramma ostriniae by constant temperature: at temperature 20-28 ℃, the corn borer oophagous trichogrammae that under the humidity 60%-90% condition gelechiid ova and emergence feed was fed is inserted numerous beehive, after connecing honeybee 24-30 hour, blow away surperficial oophagous trichogrammae and gelechiid ova (having supplied with the ovipositing hosts of corn borer oophagous trichogrammae) is collected.The collected corn borer oophagous trichogrammae that grows ovum latter stage to larva mid-term, continues under the humidity 70%-90% condition to grow at temperature 20-28 ℃ in numerous honeybee chamber.Continue to grow after 0-24 hour (being to grow about 24-54 hour in numerous honeybee chamber accumulative total after gelechiid ova connects honeybee) and pack gelechiid ova into shallow uncovered bottle, and change over to after sealing with black cloth under temperature 10.5-12.5 ℃, relative moisture 70%-90% condition and induce 5-6 week, can make 90% above corn borer oophagous trichogrammae enter the diapause attitude.
2, the low-temperature storage of diapause of Trichogramma ostriniae honeybee: after the low temperature induction, change oophagous trichogrammae over to low-temperature storage, condition of storage is temperature 2-5 ℃, and the best storage time is 3-5 month.
3, the recovery of refrigeration honeybee is grown: after refrigeration finished, according to the production demand, the 7-10 days in advance oophagous trichogrammaes with refrigeration changed over to and recover to grow to get final product, and recover developmental condition and are: temperature 20-28 ℃, and humidity 60%-90%, periodicity of illumination 14L:10D.After recovering to grow, the corn borer oophagous trichogrammae more than 90% can sprout wings in 10 days.Oophagous trichogrammae vitality after the experience refrigeration will be weaker than non-refrigeration honeybee, and spawning rate and egg laying amount are significantly increased after 10% the hydromel of feeding.
The inducing temperature of above-mentioned constant temperature inducing maize snout moth's larva oophagous trichogrammae is any steady temperature in 10.5-12.5 ℃, does not need alternating temperature to handle between induction period.
Above-mentioned to induce the condition of storage of the oophagous trichogrammae of diapause be temperature 2-5 ℃, do not need transformation temperature between the storage life, and the best storage time is 3-5 month under this condition.
Above-mentioned inducing diapause of Trichogramma ostriniae by constant temperature and storage method, oophagous trichogrammae through the recovery developmental condition after the long term storage is: temperature 20-28 ℃, humidity 60%-90%, periodicity of illumination 14L:10D.
Inducing diapause of Trichogramma ostriniae also realizes that long term storage can prolong the effective production time of oophagous trichogrammae, expands the scale of production, and reduces production costs.Be the oophagous trichogrammae of control of insect deposit capacity, also can be used for solving corn borer oophagous trichogrammae kind honeybee and preserve problem.Oophagous trichogrammae vitality after the experience refrigeration will be weaker than non-refrigeration honeybee, and spawning rate and egg laying amount are significantly increased after 10% the hydromel of feeding.
Embodiment
Embodiment 1: with July 10 this year needs 1,000,000 corn borer oophagous trichogrammaes be example, breeding diapause corn borer oophagous trichogrammae method is as follows:
1, numerous honeybee: at first January 30 with the capacity gelechiid ova and plant honeybee in proportion (40g gelechiid ova, 100,000 corn borer oophagous trichogrammae kind honeybees) insert numerous beehive, numerous honeybee room temperature is controlled between temperature 20-28 ℃, relative moisture is controlled between the 70%-90%.
2, inducing diapause of Trichogramma ostriniae: connect honeybee and blow away kind of a honeybee after 24 hours, gelechiid ova is collected be placed on the continuation growth of numerous honeybee chamber.(connect honeybee after about 50 hours) puts into shallow uncovered bottle with oophagous trichogrammae to connect behind the honeybee the 3rd day, changes over to after sealing with black cloth to be controlled under temperature 10.5-12.5 ℃, humidity 70%-90% condition to begin to induce the oophagous trichogrammae diapause.
3, the storage of diapause oophagous trichogrammae: after inducing 35 days under 10.5-12.5 ℃ of the room temperature of control, most oophagous trichogrammaes have entered diapause worm attitude, oophagous trichogrammae are changed over to from temperature 10.5-12.5 ℃ begin refrigeration under the 2-3 ℃ of condition.
4, deepfreeze is after 3 months, use preceding 9 days of honeybee at needs, the corn borer oophagous trichogrammae after the refrigeration is taken out down from refrigerated condition, be placed on 22 ℃ of temperature, humidity 60%, periodicity of illumination 14L:10D recovers down to grow and makes the honeybee card that the field hangs up or put into according to purposes and raises box and further feed.For example estimate July 10 and put honeybee, will refrigerate honeybee July 1 and take out, and it is standby to make the honeybee card in 5 days; According to putting into some raising boxes respectively after the total honeybee of the parasitic rate bigness scale amount, before emergence, to give to add 10% hydromel in the box and get food for the oophagous trichogrammae that sprouts wings, oophagous trichogrammae sprouts wings to get and can connect honeybee in proportion after the food and expand numerous.
Embodiment 2: the primary condition of inducing diapause of Trichogramma ostriniae by constant temperature and storage method is identical with embodiment 1 with step, and the condition of storage of wherein inducing the oophagous trichogrammae of diapause is temperature 3-5 ℃, and the storage time is 5 months.The oophagous trichogrammae of long term storage recovers developmental condition: 28 ℃ of temperature, humidity 90%, periodicity of illumination 14L:10D.
Claims (1)
1. inducing diapause of Trichogramma ostriniae by constant temperature and storage method is characterized in that this method comprises:
(1) inducing diapause of Trichogramma ostriniae by constant temperature: the corn borer oophagous trichogrammae that under temperature 20-28 ℃, humidity 60%-90% condition gelechiid ova and emergence feed was fed is inserted numerous beehive and connects honeybee, the corn borer oophagous trichogrammae that ovum latter stage to larva mid-term is grown in collection continues to grow under 20-28 ℃ of numerous honeybee room temperature, humidity 70%-90% condition, continue to grow after 0-24 hour, gelechiid ova changed under temperature 10.5-12.5 ℃, relative moisture 70%-90% condition induce 5-6 week, make the corn borer oophagous trichogrammae enter the diapause attitude;
(2) low-temperature storage of diapause of Trichogramma ostriniae honeybee: after the low temperature induction, change oophagous trichogrammae over to low-temperature storage, the condition of low-temperature storage is 2-5 ℃, storage time 3-5 month;
(3) recovery of refrigeration honeybee is grown: after refrigeration finished, according to the production demand, the 7-10 days in advance oophagous trichogrammaes with refrigeration changed over to and recover to grow to get final product, and recover developmental condition and are: temperature 20-28 ℃, and humidity 60%-90%, periodicity of illumination 14L:10D.
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Cited By (1)
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| CN103141443A (en) * | 2013-03-08 | 2013-06-12 | 中国农业科学院草原研究所 | Method for regulating and controlling diapause of agryponflexorius |
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| CN104041462A (en) * | 2014-07-03 | 2014-09-17 | 湖南农业大学 | Method for regulating and controlling diapause of microplitis syntretomorpha |
| CN104255660A (en) * | 2014-09-01 | 2015-01-07 | 吉林农业大学 | Method for alternately storing trichogramma at intermediate and low temperature in two steps |
| CN104255669B (en) * | 2014-09-24 | 2017-01-18 | 中国农业科学院植物保护研究所 | Diapause induction method of harmonia dimidiata |
| CN104351140B (en) * | 2014-09-24 | 2017-10-17 | 中国农业科学院植物保护研究所 | Red shoulder ladybug Diapause termination method |
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Patent Citations (3)
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|---|---|---|---|---|
| CN85100223A (en) * | 1985-04-01 | 1985-09-10 | 武汉大学 | Raise artificial host egg's card of oophagous trichogrammae |
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| CN103141443A (en) * | 2013-03-08 | 2013-06-12 | 中国农业科学院草原研究所 | Method for regulating and controlling diapause of agryponflexorius |
| CN103141443B (en) * | 2013-03-08 | 2015-09-16 | 中国农业科学院草原研究所 | A kind of regulate and control the method for beet webworm Ah lattice ichneumon wasp diapause |
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