CN108450419A - A kind of Trichogramma chilonis Diapause inducement method - Google Patents
A kind of Trichogramma chilonis Diapause inducement method Download PDFInfo
- Publication number
- CN108450419A CN108450419A CN201810185170.XA CN201810185170A CN108450419A CN 108450419 A CN108450419 A CN 108450419A CN 201810185170 A CN201810185170 A CN 201810185170A CN 108450419 A CN108450419 A CN 108450419A
- Authority
- CN
- China
- Prior art keywords
- bee
- trichogramma
- diapause
- trichogramma chilonis
- relative humidity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000521156 Trichogramma chilonis Species 0.000 title claims abstract description 29
- 230000005058 diapause Effects 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 10
- 241000256844 Apis mellifera Species 0.000 claims abstract description 23
- 241000753145 Sitotroga cerealella Species 0.000 claims abstract description 18
- 238000005286 illumination Methods 0.000 claims abstract description 10
- 230000003071 parasitic effect Effects 0.000 claims abstract description 10
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 6
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 6
- 210000004681 ovum Anatomy 0.000 claims abstract description 6
- 238000012258 culturing Methods 0.000 claims abstract description 5
- 210000001161 mammalian embryo Anatomy 0.000 claims abstract description 5
- 241000256618 Trichogramma Species 0.000 claims description 6
- 210000004894 snout Anatomy 0.000 claims description 3
- 230000001939 inductive effect Effects 0.000 claims 1
- 241000238631 Hexapoda Species 0.000 abstract description 12
- 238000003860 storage Methods 0.000 abstract description 7
- 206010010741 Conjunctivitis Diseases 0.000 abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 230000007774 longterm Effects 0.000 abstract description 3
- 238000009395 breeding Methods 0.000 abstract description 2
- 230000001488 breeding effect Effects 0.000 abstract description 2
- 241000607479 Yersinia pestis Species 0.000 description 5
- 239000000575 pesticide Substances 0.000 description 4
- 235000013305 food Nutrition 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241000426497 Chilo suppressalis Species 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 241000008892 Cnaphalocrocis patnalis Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241000255967 Helicoverpa zea Species 0.000 description 1
- 206010054949 Metaplasia Diseases 0.000 description 1
- 241000346285 Ostrinia furnacalis Species 0.000 description 1
- 241000500437 Plutella xylostella Species 0.000 description 1
- 230000009418 agronomic effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000021393 food security Nutrition 0.000 description 1
- 230000000266 injurious effect Effects 0.000 description 1
- 238000007726 management method Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000015689 metaplastic ossification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention discloses a kind of Trichogramma chilonis Diapause inducement method, in 25 DEG C of temperature, relative humidity 70% 90%, illumination L8：It by Trichogramma chilonis honeybee kind and kills the rice moth egg after embryo under conditions of D16 or completely black is dark and is placed in bee-receiving box according to the ratio of bee ovum ratio 1: 20 30；It is removed after 12 16h into bee, it is 25 DEG C that parasitic rice moth egg, which is put into temperature, relative humidity 70% 90%, illumination L8：Continue culture 12 in culturing room dark D16 or completely black for 24 hours；It is 10 11 DEG C that parasitic rice moth egg, which is transferred to temperature, relative humidity 70% 80%, illumination L8：It is induced 30 40 days under conditions of D16 or completely black is dark, Trichogramma chilonis can enter diapause status.The present invention can be used for the Trichogramma chilonis of long-term production storage rice moth egg breeding, lay in enough cute conjunctivitis bee products for control of insect, it can also be used to solve Trichogramma chilonis honeybee kind storage problem.
Description
Technical field
The present invention relates to agricultural pests field of biological control, and in particular to a kind of Trichogramma chilonis Diapause inducement method.
Background technology
The global problems such as environmental protection, bio-diversity, food security promote national governments to go into overdrive strictly to limit
The use of chemical pesticide.Biological control, the comprehensive treatment realization of diseases and pests of agronomic crop are greatly lowered chemical pesticide and use
Main path.China's " pollution-free food and biological pesticide " is classified as 21 century by government and first develops agenda, biological control of insect pests
Also it is increasingly taken seriously.The utilization of natural enemy insect is the important measures of sustainable pest management, passes through a large amount of of natural enemy insect
Rationally release can increase the population quantity of the initial natural enemy in field, substitute or reduce the use of chemical pesticide, have become nuisanceless
Change, the main means of pollution-free food production.
Trichogramma is to apply a kind of parasitic enemy insect that area is most wide, controlling object is most in the world at present, in life
There is important application value in object prevention.Trichogramma chilonis (Trichogramma chilonis) is one kind of trichogramma, right
A variety of agriculture and forestry injurious insects have good control effect, are widely used in prevention rice-stem borer, corn borer, bollworm, the soybean food heart
The agricultural pests such as worm, rice leaf roller, diamondback moth, sugarcane borer have very high application value.Currently, the utilization of trichogramma is still
Submerged release is relied primarily on, this delivery mode needs a large amount of cute conjunctivitis bee product, the cute conjunctivitis of conventional storage in the specific period
Bee storage stability is poor, cannot meet the needs of large area biological control.Diapause is to be widely present in a kind of physiology of insect world
Ecological phenomenon, is the important life history strategy that insect spends poor environment, and many insects maintain population and individual by diapause
Existence.After insect enters diapause status, not specially treated termination of diapause generally can be in the slow of low metabolism the long period
Development, or developmental condition is stagnated, diapause insect can survive for a long time not feeding at this time.Using resistance to after Diapause of Trichogramma
The characteristics of storage, induces Trichogramma chilonis diapause and realizes long term storage, can extend the shelf life of cute conjunctivitis bee product, reduces life
Cost is produced, expands the scale of production, meets the needs of large area biological control is to cute conjunctivitis bee product.In the prior art there are no at
The Trichogramma chilonis of the parasitic rice moth egg of work(induction enters the report of diapause.
Invention content
To solve the above problems, the present invention provides a kind of Trichogramma chilonis Diapause inducement method, it can be used for long-term production
The Trichogramma chilonis for storing rice moth egg breeding lays in enough cute conjunctivitis bee products, it can also be used to it is red to solve snout moth's larva Huang for control of insect
Eye bee honeybee kind storage problem.
To achieve the above object, the technical solution that the present invention takes is：
A kind of Trichogramma chilonis Diapause inducement method, includes the following steps：
In 25 DEG C, relative humidity 70%-90%, illumination L8 of temperature：By Trichogramma chilonis bee under conditions of D16 or completely black is dark
The rice moth egg after embryo is planted and kills to be placed in bee-receiving box according to the ratio of bee ovum ratio 1: 20-30；It is removed at bee, by rice moth after 12-16h
It is 25 DEG C, relative humidity 70%-90%, illumination L8 that ovum, which is put into temperature,：Continue to cultivate 12- in culturing room dark D16 or completely black
24h；Then it is 10-11 DEG C, relative humidity 70%-90%, illumination L8 rice moth egg to be transferred to temperature：Condition dark D16 or completely black
Lower induction 30-40 days, Trichogramma chilonis can enter diapause status, and Diapause rate reaches 90% or more.
The present invention realizes the regulation and control to the one Trichogramma chilonis diapause of excellent parasite, in fact by manipulating environmental factor
The stored for extended periods of existing Trichogramma chilonis, extends the shelf life of Trichogramma chilonis, reduces production cost, is Trichogramma chilonis
Anniversary metaplasia production and large area Field information provide technical guarantee.
Specific implementation mode
In order to make objects and advantages of the present invention be more clearly understood, the present invention is carried out with reference to embodiments further
It is described in detail.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to limit this hair
It is bright.
Embodiment 1
A kind of Trichogramma chilonis Diapause inducement method, feature is, includes the following steps：
In 25 DEG C, relative humidity 70%-90%, illumination L8 of temperature：Trichogramma chilonis honeybee kind and embryo will be killed under conditions of D16
Ratio of the rice moth egg afterwards according to bee ovum than 1: 20-30 is placed in bee-receiving box；It is removed into bee after 12-16h, parasitic rice moth egg is put
It is 25 DEG C, relative humidity 70%-90%, illumination L8 to enter temperature：Continue to cultivate 12-24h in the culturing room of D16；It then will be parasitic
It is 10-11 DEG C, relative humidity 70%-90%, illumination L8 that rice moth egg, which is transferred to temperature,：It is induced 30-40 days under conditions of D16, snout moth's larva is yellow
Trichogramma can enter diapause status, and Diapause rate reaches 90% or more.
Embodiment 2
A kind of Trichogramma chilonis Diapause inducement method, includes the following steps：
In 25 DEG C of temperature, relative humidity 70%-90%, it is completely black it is dark under conditions of by Trichogramma chilonis honeybee kind and after killing embryo
Ratio of the rice moth egg according to bee ovum than 1: 20-30 is placed in bee-receiving box；It is removed into bee after 12-16h, parasitic rice moth egg is put into temperature
Degree is 25 DEG C, relative humidity 70%-90%, continues to cultivate 12-24h in completely black dark culturing room；Then parasitic rice moth egg is turned
It is 10-11 DEG C, relative humidity 70%-90% to enter temperature, it is completely black it is dark under conditions of induce 30-40 days, Trichogramma chilonis can be into
Enter diapause status, Diapause rate reaches 90% or more.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, without departing from the principle of the present invention, it can also make several improvements and retouch, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (2)
1. a kind of Trichogramma chilonis Diapause inducement method, feature is, includes the following steps：
S1, under conditions of 25 DEG C of the temperature, relative humidity 70%-90% by Trichogramma chilonis honeybee kind and kill the rice moth egg after embryo by
Ratio according to bee ovum than 1: 20-30 is placed in bee-receiving box, is removed into bee after 12-16h；
S2, parasitic rice moth egg is put into temperature is 25 DEG C, continues in the culturing room of relative humidity 70%-90% to cultivate 12-24h；
S3, parasitic rice moth egg is transferred to temperature is 10-11 DEG C, is induced 30-40 days under conditions of relative humidity 70%-80%, snout moth's larva
Yellow trichogramma can enter diapause status, and Diapause rate reaches 90% or more.
2. a kind of Trichogramma chilonis Diapause inducement method as described in claim 1, feature are, described step S1, S2 and
The inductive condition of S3 is illumination L8：D16 or completely black is dark.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810185170.XA CN108450419A (en) | 2018-02-26 | 2018-02-26 | A kind of Trichogramma chilonis Diapause inducement method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810185170.XA CN108450419A (en) | 2018-02-26 | 2018-02-26 | A kind of Trichogramma chilonis Diapause inducement method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108450419A true CN108450419A (en) | 2018-08-28 |
Family
ID=63216753
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810185170.XA Pending CN108450419A (en) | 2018-02-26 | 2018-02-26 | A kind of Trichogramma chilonis Diapause inducement method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108450419A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1397159A (en) * | 2002-09-04 | 2003-02-19 | 衡水田益生防有限责任公司 | Technology for industrially culturing trichogramma with gelechiid ova |
| JP2008113605A (en) * | 2006-11-06 | 2008-05-22 | Nakamura Kazuo | Method for breeding, supplying and raising japanese indigenous bumblebees |
| CN101326902A (en) * | 2008-07-04 | 2008-12-24 | 衡水田益生防有限责任公司 | Method for inducing diapause of Trichogramma ostriniae by constant temperature and storage method |
| CN105766803A (en) * | 2016-03-15 | 2016-07-20 | 吉林农业大学 | Method for blended breeding of trichogramma dendrolimi and trichogramma ostriniae with oak silkworm eggs as hosts |
-
2018
- 2018-02-26 CN CN201810185170.XA patent/CN108450419A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1397159A (en) * | 2002-09-04 | 2003-02-19 | 衡水田益生防有限责任公司 | Technology for industrially culturing trichogramma with gelechiid ova |
| JP2008113605A (en) * | 2006-11-06 | 2008-05-22 | Nakamura Kazuo | Method for breeding, supplying and raising japanese indigenous bumblebees |
| CN101326902A (en) * | 2008-07-04 | 2008-12-24 | 衡水田益生防有限责任公司 | Method for inducing diapause of Trichogramma ostriniae by constant temperature and storage method |
| CN105766803A (en) * | 2016-03-15 | 2016-07-20 | 吉林农业大学 | Method for blended breeding of trichogramma dendrolimi and trichogramma ostriniae with oak silkworm eggs as hosts |
Non-Patent Citations (1)
| Title |
|---|
| 李丽英等: "低温诱导赤眼蜂滞育与寄主的关系", 《昆虫天敌》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Nepi et al. | Nectar in plant–insect mutualistic relationships: from food reward to partner manipulation | |
| Lu et al. | Rice pest management by ecological engineering: a pioneering attempt in China | |
| Long et al. | Mechanism and capacities of reducing ecological cost through rice–duck cultivation | |
| Khan et al. | Assessment of the potential of Napier grass (Pennisetum purpureum) varieties as trap plants for management of Chilo partellus | |
| Zhu et al. | Selection of nectar plants for use in ecological engineering to promote biological control of rice pests by the predatory bug, Cyrtorhinus lividipennis,(Heteroptera: Miridae) | |
| CN103891659B (en) | Method for breeding Odontobutis obscura through fish-rice symbiosis in pool | |
| Qualset et al. | In California:‘Agrobiodiversity’key to agricultural productivity | |
| Sable et al. | Impact of global warming on insect behavior-A review | |
| CN103766324B (en) | Fructus Jujubae garden utilizes the method for red neck normal room cocoon honeybee Biological control green plant bug | |
| Goulson | Bumblebees | |
| Altieri et al. | Vegetational designs to enhance biological control of insect pests in agroecosystems | |
| Adunola et al. | Impact of climate change on armyworm infestation on maize in Nigeria: A review | |
| Singh et al. | Ant attendance of the cotton aphid is beneficial for okra plants: deciphering multitrophic interactions | |
| Huang et al. | Changes in the photosynthetic characteristics of cotton leaves infested by invasive mealybugs tended by native ant species | |
| CN108450419A (en) | A kind of Trichogramma chilonis Diapause inducement method | |
| Boopal et al. | Potato a suitable alternative host for brinjal fruit shoot and fruit borer Leucinodes orbonalis Guenee (Lepidoptera: Pyralidae). | |
| CN104996366A (en) | Trichogramma japonicum high-efficiency reproduction method | |
| Srinivas et al. | Bio-efficacy of insecticides against fruit fly, Bactrocera cucurbitae (Coquillett) in cucumber | |
| Huang et al. | Integrated control of sugarcane diseases and pests | |
| CN108902054A (en) | A kind of mythimna separata manual intelligent cultural method | |
| Chandana et al. | Biology of green lacewing, Chrysoperla zastrowi sillemi (Esben-Petersen) on cabbage aphid, Brevicoryne brassicae L. | |
| Ogah et al. | Biological control of African rice gall midge (Orseolia oryzivora, Harris and Gagné) in Nigeria: a review | |
| Stavridis et al. | Deviations from prediction model of predatory effect for Coccinellidae | |
| Handiseni et al. | Pest resistance management strategies: A mini review of the case of cotton (Gossypium hirsutum) in Zimbabwe | |
| Li et al. | Mass production and application of natural enemy insects in China |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180828 |
|
| RJ01 | Rejection of invention patent application after publication |