JP2008113605A - Method for breeding, supplying and raising japanese indigenous bumblebees - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a method for breeding, supplying and raising indigenous Japanese bumblebees by which the bumblebees can be raised, while making entering of a new queen bee after matting, into diapause of several months, averted. <P>SOLUTION: The method for breeding, supplying and raising the indigenous Japanese bumblebees is carried out via at least a first step for obtaining worker bees from the queen bee of the indigenous Japanese bumblebees in a small box 15; a second step for enabling eggs to make the resulting bees emerge and be raised by colonies; a third step for making the new queen bee, after the raising, to mate with male bees; a fourth step for subjecting the new queen bee after the copulation to a prescribed diapause treatment; a fifth step for raising the new queen bee, after the diapause; and a sixth step for making the method return to the first step, when the new queen bee starts laying eggs, by moving the new queen bee into the nest chamber 23 of the small box 15. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a Japanese native bumblebee breeding and feeding method suitable for use as a pollinator that performs artificial pollination of tomatoes and the like grown in the facility to collect Japanese native bumblebees that collect pollen by vibration milling It is.

  Unlike the natural world, eggplants such as tomatoes that do not secrete nectar cultivated in the facility require extra work, such as spraying phytohormonal agents, to bring fruit to fruition. A labor force is required and other artificial pollination methods have been developed from the viewpoint of reducing the burden.

  One of the methods is to install a bee hive bee hive in the cultivation facility to perform pollination work. However, the giant bee is an alien species, and when it escapes from the facility, there is a high risk of adverse effects on the Japanese natural environment and ecosystem.

For this reason, Japanese native bumblebee breeding methods that artificially breed Japanese bumblebees that do not have the risk of adversely affecting Japanese ecosystems and open the path to mass growth have already been proposed (Patent Literature). 1).
Japanese Patent No. 3421121

  Patent Document 1 discloses a breeding method in which one queen bee of a Japanese native bumblebee that was mated with 1 to 10 worker bees of a bumblebee as a helper is raised. In this case, the new queen bee after mating can be prevented from entering dormancy for several months after mating by attaching a worker bee, a worker bee, as a helper. It is said that bumblebees can be produced systematically in Japan.

  However, in the case of the disclosed technique of Patent Document 1, the new queen bee after mating must have a bee that is a worker bee as a helper. there were.

  In view of the above-mentioned problems of the prior art including Patent Document 1, the present invention is a Japanese native that can prevent a new queen bee after mating from entering a dormancy for several months without attaching a bumblebee as a helper. It aims at providing the breeding supply breeding method of bumblebees.

  The present invention has been made to achieve the above-mentioned object, and has finished mating with bumble bees of Japanese native bumblebees in the nesting chamber of a small box having a nesting chamber and a feeding chamber that are partitioned freely. The first step of obtaining Japanese bee queen bees and laying eggs to obtain working bees, and about 10 working bees in the breeding room of a large box having a nesting room and a feeding room partitioned freely. The second step of transferring the colonies to a colony and raising them as a bee group under a temperature state of 23 degrees Celsius or higher, and a new one among the bee groups reared in the second step. The third step of taking out and mating the queen bee and the male bee, the fourth step of applying a dormancy process to the new queen bee that has finished mating, and the plurality of new queen bees that have undergone the fourth step in the cage Rearing competing 5 steps, and when the new queen bees that have passed through the fifth step start laying eggs, the individual new queen bees are transferred to the nesting chamber of the small box maintained at a temperature of about 27 ° C. and returned to the first step. The main feature is to include at least a sixth step.

  In this case, in the first step, it is preferable to promote the nesting by fixing a bumble bee of a Japanese native bee to the nesting room with a wax. In addition, it is desirable to use a large box whose bottom is formed of a mesh material.

On the other hand, the dormancy treatment in the fourth step is a 1: 1 addition of 10% bone charcoal to soil obtained by mixing 30% multi-functional bio soil with 70% breeding soil and humus soil. A pre-treated soil made by spraying 6 ppm nano silver-supported collagen water on the mixture mixed in a ratio is laid in a closed space to a thickness of 1 to 2 cm, and then the new queen bee that has undergone the third step is accommodated. Processing,
The queen bee after being accommodated in the closed space is allowed to sleep in a low temperature state of 8 to 10 ° C. for about 1 to 3 weeks, and the queen bee after the sleep is taken out, gradually raised in temperature, and finally Can be performed at least through a treatment of 25 to 28 ° C.

  In addition, the dormancy treatment in the fourth step is performed by examining individual differences of the new queen bee that has undergone the third step in water at 25 to 30 ° C. in which 0.1 cc of 6 ppm nanosilver-carrying collagen water is mixed with 100 cc of water. While being submerged until it becomes a dead state, removing water adhering to the body surface after removing from water, and removing the water, it is immediately put in a closed space of 25-30 ° C. and becomes nano silver serving as food The first feeding flight process in which the sugar solution and pollen to which the supported collagen is added is given and the above process including submersion is repeated until the next process is repeated and the same process as the first feeding flight process is performed within one week 2 It may be performed through at least the second feeding flight process.

  Furthermore, the dormancy process in the fourth step includes a process of accommodating the new queen bee that has passed through the third step in a closed space, and the new queen bee stops its movement and becomes anesthetized. A process of supplying carbon dioxide gas in the closed space, leaving it in the atmosphere for 30 to 70 minutes, taking it out and raising it for several days, and then storing the queen bee in the closed space again, Carbon dioxide gas may be supplied into the closed space until it stops moving and becomes anesthetized, and may be removed through at least 30 to 70 minutes and then removed. .

  According to the first aspect of the present invention, the queen bee of Japanese bumblebees lays eggs, emerges and mates with the bumblebees of Japanese bumblebees, and then the dormant treatment is applied to the new queen bees. By avoiding overwintering, Japanese bumblebees can be bred systematically while realizing generational changes with a cycle shorter than one year.

  Therefore, in the cultivation facility of solanaceous plants such as tomatoes, the Japanese native bumblebees should be used as a pollinator for artificial pollination by installing a nest box formed with colonies made of Japanese native bumblebees. Can do. Moreover, since the bumblebees used are native to Japan, even if they escape from the facility, there is no risk of serious adverse effects on the Japanese ecosystem.

  According to the invention of claim 2, since the bumble bee of Japanese bumblebees is fixed in the nesting room with the wax, the nesting of the queen bee in the first step can be further promoted.

  According to invention of Claim 3, since the bottom face of the large box which has a nesting room and a feeding room is formed with the net material, the working bee in the 2nd step can be reared more hygienically as a bee group. .

  According to the invention of claim 4, the sleep process in the fourth step is performed in a low temperature state by accommodating the queen bee that has finished mating after laying the pretreated soil in a closed space to a thickness of 1 to 2 cm. Since it is performed by gradually raising the temperature after sleeping, the new queen bee can avoid overwintering and start laying eggs while effectively preventing mold. Moreover, basically overwintering can be avoided if it is carried out for more than two weeks, and there is no particular problem even if the processing is continued further.

  According to the invention of claim 5, the dormancy process in the fourth step is to submerge the queen bee until it becomes a temporary death state and take it out into a closed space of 25 to 30 ° C. Same as the first feeding and flying treatment within one week, and the first feeding and flying treatment in which the added sugar solution (to prevent mold generated in the sugar solution and to remove viruses etc.) and pollen are given. By carrying out the novel method of passing through the second feeding and flying process for performing the treatment easily, the new queen bee can avoid the overwintering and start the egg laying.

  According to the invention of claim 6, a new nap treatment using carbon dioxide is applied to a new queen bee of Japanese bumblebees that has finished mating with a bumblebee of Japanese bumblebees. The queen bee can avoid spawning and start spawning.

  FIG. 1 is an overall perspective view showing an example of a net gauge in which a new queen bee is temporarily accommodated in the course of the method of the present invention. According to the figure, the gauge 11 can be used to temporarily insert a new queen bee that is in the process of dormancy, to fly a new queen bee after mating, or to promote spawning. A chamber 12 is provided.

  The method of the present invention comprises a first step of obtaining a worker bee from a queen bee of Japanese native bumblebees including a Japanese native bee, a second step of raising eggs by colony and rearing, and a new queen bee and a male bee after breeding. The third step of mating, the fourth step of applying a predetermined dormancy treatment to the new queen bee after mating, the fifth step of breeding the new queen bee after the dormancy treatment, and a small time when the new queen bee starts laying eggs It is performed by passing through at least the sixth step of moving into the nesting chamber 24 of the box 21 and returning to the first step.

  Of these, the first step is to place a Japanese native bumblebee queen bee that has finished mating with a Japanese native bumblebee male bee in the nesting room of a small box having a nesting room and a feeding room partitioned freely. It is prepared for putting and laying eggs to obtain working bees.

  That is, in the first step, the bee queen of Japanese bumblebees that have finished mating with the bumblebee of Japanese bumblebees using the small box 15 shown in FIG. Get.

  The small box 15 used in this case has an opening surface 17 as an upper surface and a box body 16 having a plurality of vent holes 16a having a diameter of about 3 mm, and a transparent top plate that covers the opening surface 17 so as to be freely opened and closed. 28.

  Of these, the box body 16 is composed of a bottom plate 18 made of agathis material having a length of 90 mm, a width of 180 mm, and a thickness of 8 mm, and an agathis material having a height of 60 mm, a width of 150 mm, and a thickness of 8 mm arranged upright on the bottom plate. Bonding for woodworking front and rear side plates 19 and left and right side plates 20 made of agathis material having a height of 60 mm, a width of 74 mm, and a thickness of 8 mm, which are positioned between the side plates 19 and 19 and arranged upright on the bottom plate 18 It is formed by adhering using an agent and a nail material 21 made of stainless steel nails or the like.

  Moreover, the box body 16 has an internal space partitioned at a central position in the width direction by a partition plate 22 made of agathis material having a height of 60 mm, a width of 74 mm, and a thickness of 8 mm. The right side can be secured as the feeding chamber 24. In addition, the partition plate 22 includes a notch 22 a at the lower corner of the back side so that a worker bee after emergence can move between the nesting chamber 23 and the feeding chamber 24.

  Further, the box body 16 has a notch 20a formed at the lower center of the right side plate 20, and an opening 25 secured between the notch 20a and the bottom plate 18 projecting in the shape of a tongue. The feeder 26 containing the sugar solution can be inserted through the insertion portion 26a. The insertion portion 26a side is covered with a net member 27 made of a stainless steel net or the like so that worker bees and the like do not escape.

  The transparent top plate 28 includes a left top plate portion 28a made of a transparent acrylic resin having a thickness of 2 mm that covers the left opening surface divided by the partition plate 22, and a transparent acrylic film having a thickness of 2 mm that covers the right opening surface. It is composed of a right top plate portion 28b made of resin, and each is disposed so as to be openable and closable via a stainless hinge 29.

  In the nesting chamber 23 of the small box 15 formed in this way, a moth (not shown) of a bumblebee native to Japan is fixed on the bottom plate 18 with a wax, and the nesting of a queen bee can be promoted. It is like that.

  In the nesting chamber 23, about 60% sucrose solution (including a mixture of 0.1 cc of nanosilver-carrying collagen water with respect to 100 cc of sugar solution) and undried fresh pollen or the A dumpling-shaped pollen made from fresh pollen is placed on the cap. Further, in the feeding chamber 24, about 60% sugar solution (such as sucrose solution) (for prevention of mold generated in the sugar solution and removal of viruses etc., nanosilver-supported collagen water with respect to 100 cc of the sugar solution) An insertion portion 26a of a feeder 26 containing a mixture of 0.1 cc) is placed.

  In the second step, when there are about 10 working bees in the nesting room 34 in the bin 31 having the nesting room 34 and the feeding room 35 that are freely detachable, the colony 36 is transferred to each colony 36 and 23 degrees Celsius or more. The colony 36 is emerged under the temperature condition, and is prepared for breeding as a bee group.

  That is, in the second step, when there are about 10 working bees in the small box 15, a colony (not shown) that has grown sufficiently is taken out from the small box 15 together with a plate piece and used as a hive box as shown in FIG. (For example, 20 cm in length × 28 cm in width × 13 cm in depth) The colony 36 is emerged by moving into a suitable temperature state of 23 degrees Celsius or more after being transferred to the inside 31.

  The large box 31 used in this case is formed using a cedar board having a thickness of 1 cm. The inside of the large box 31 is partitioned into a nesting chamber 34 and a feeding chamber 35 through a partition plate 33 having a through hole 33a having a diameter of about 2 cm through which a bee can pass in the same manner as the small box 11 of FIG. A colony 36 is placed together with a plate piece 44 in the chamber 34. In addition, when the bottom 31a of the large box 31 is formed with a net material made of stainless steel net or the like, it can be dropped naturally through the net material without depositing excrement etc., so it is more hygienic. A breeding environment can be secured.

  In the nesting chamber 34, fresh pollen that has not been kneaded is placed in a cap similar to that used in the small box 11, or kneaded pollen 40 is placed directly on the bee after emergence. Gave. In addition, the top plate 32 that covers the upper surface opening of the large box 31 is formed using a transparent acrylic plate having a large number of ventilation holes (not shown), so that the bees inside do not escape. For example, it is fastened with a gummed tape 37 or the like.

  A sucrose solution 43 containing a mixture of 100 cc of sugar solution at a ratio of 0.1 cc of nanosilver-carrying collagen water is used to prevent mold generated in the sugar solution and remove viruses and the like. An insertion hole 39 was formed in the side plate 38, and a feeder 41 was inserted from the outside through the insertion hole 39 so as to be given to the bees inside. In this case, about 60% of the sucrose solution 43 was supplied to the feeder 41 after covering the insertion part 41a with a net member 42 made of stainless steel net or the like so that the bees would not escape outside. In addition, it is necessary to change the bait once every two days so that the bait is not cut. In addition, since the feeding chamber 35 is also a place where excrement and the like are accumulated, it is necessary to clean the inside of the feeding chamber 35 at the time of changing the feed so that the inside is always kept clean.

  The large box 31 used as a hive was placed in a room where the room was kept at 23 to 27 ° C. and humidity of about 50%. At that time, bone charcoal was placed in the nest to maintain an appropriate humidity.

  The third step is prepared for taking out and mating the new queen bee and the male bee from the bee group reared in the second step.

  That is, in the third step, the new queen bee and the male bee are taken out from the bee group that emerged from the colony 36 in the second step and mated. The mating was performed using the net gauge 11 shown in FIG. Since the gauge 11 includes three compartments 12, it can be used efficiently. Each compartment 12 was mated with more than twice as many male bees as the number of new queen bees.

  In this case, the gauges 11 were mated indoors by arranging them under a window side into which sunlight was inserted or a fluorescent tube close to the wavelength of sunlight. When a fluorescent tube close to the wavelength of sunlight is used, the mating rate can be increased even in rainy or cloudy weather. The mated pair was removed from the compartment 12 and separated. In addition, the new queen bee after mating is transferred to the compartment 12 of the gauge 11 having the same structure and prepared in the room, and about 60% sucrose solution (to prevent mold generated in the sugar solution and In order to remove viruses and the like, a sugar solution (including a mixture of 0.1 cc of nanosilver-supported collagen water with 100 cc of water) and kneaded pollen were given.

  In the fourth step, dormancy processing is performed on the new queen bee that has finished mating. The dormancy processing method includes the following three methods, which can be appropriately selected as desired.

  The first method is to add an antibacterial substance that is harmless to insects to a mixture of soil and humus soil in a 1: 1 ratio, which is a mixture of breeding soil and land stabilization soil (multifunctional bio soil). Japanese bee queen bee that finished mating with Japanese bee bumble bee in the third step after laying pre-treated soil with sprayed water in a closed space to a thickness of 1-2 cm , A process of allowing the queen bee after being housed in the closed space to sleep in a low temperature state of 8 to 10 ° C. for about 1 to 3 weeks, and taking out the queen bee after the sleep, It is performed by raising the temperature and finally passing through a treatment of 25 to 28 ° C.

  In this case, as the breeding soil, a sintered granular volcanic ash substrate soil that is porous but has sufficient hardness from the viewpoint of maintaining an appropriate pH value and maintaining endurance is used. The soil produced by the “method for producing floor soil or filter medium” according to Japanese Patent No. 2917140 can be suitably used. In addition, as for the landing stable soil, the soil obtained as “bottom soil and filter medium of appreciation fish tank” according to Japanese Patent No. 2603202 can be suitably used from the viewpoint of obtaining stable pH value.

  Moreover, by combining nano-silver (antibacterial substance) with the breeding soil and the landing stable nourishment soil, it is possible to create a more stable nest when used as a nest floor of Japanese bumblebees. Become. In this case, for example, 10% bone charcoal is added to the soil obtained by mixing 30% landing stable nutrient soil (multifunctional bio-soil) with 70% breeding soil and humus soil at a ratio of 1: 1. A pretreated soil obtained by spraying 6 ppm of nanosilver-supported collagen water on the mixture can be suitably used. Bone charcoal is a component that helps to prevent mold in enclosed spaces and at the same time maintain moderate humidity regardless of outside air temperature and humidity, and also helps prevent salt replenishment and the generation of mites and viruses. Become.

  The second technique is to submerge the Japanese bumblebee queen bee, which has finished mating with the bumblebee of Japanese bumblebee in the third step, in a temperature of 25 to 30 ° C. Treatment, removing water adhering to the body surface after being taken out from water, and immediately after removing the water, put it in a closed space of 25-30 ° C. For prevention of mold and removal of viruses, etc., sugar solution (including 100 cc of water mixed with 0.1 cc of nanosilver-supported collagen water) and pollen are given and submerged until the next treatment It is performed through at least a first feeding flight process for flying while repeating the above process and a second feeding flight process for performing the same process as the first feeding flight process within one week.

  In the third method, the new queen bee of the Japanese bumblebee that finished mating with the bumblebee of the Japanese bumblebee in the third step is accommodated in the closed space, and the movement of the new queen bee stops. Carbon dioxide gas is supplied into the closed space until an anesthesia is applied, and after leaving it in the atmosphere for 30 to 70 minutes, it is taken out and bred for several days, and then the new queen bee is again treated. After being accommodated in the closed space, the carbon dioxide gas is supplied into the closed space until the new queen bee stops its movement and becomes anesthetized, and left in the atmosphere for 30 to 70 minutes. The process is performed through at least the extraction process.

  In particular, in the third method, the present inventors discovered that the sensitivity of Japanese black bee to carbon dioxide anesthesia is different from that of Japanese bee wasp, and this finding (the effect of anesthesia because of the early awakening after anesthesia). Based on the assumption that the anesthesia treatment time using carbon dioxide was changed based on the assumption that it was weak, it was found that it was possible to rear the animal more reliably, and it came to completion.

  That is, first of all, the new queen bee of the Japanese black bee that has finished mating with the Japanese bee of the Japanese black bee is formed of three compartments 12 with a height of 18 cm and a height and width of 35 cm each, for example. 1 is placed in an empty compartment 12 of the net gauge 11 shown in FIG. 1, and about 60% sucrose solution and freshly dried raw pollen or kneaded pollen with the fresh pollen are placed on the cap. It is put in a state where it is placed, and after flying for one week while giving it, it is accommodated in a closed space of a container (not shown) that can be freely covered with a lid such as a test tube.

  Next, a tube drawn out from the carbon dioxide cylinder is inserted through a gap between the lids of the container, and carbon dioxide is poured into the closed space. In this case, the carbon dioxide is supplied until the contained new queen bee stops its movement and becomes anesthetized.

  When the movement of the new queen bee stops, after confirming its state, the container is firmly covered and left in the atmosphere for 30 to 70 minutes. After the standing time, the container is opened and the new queen bee is taken out from the closed space.

  The removed new queen bee is placed in a vacant compartment 12 in the gauge 11 shown in FIG. 1, and about 60% sucrose solution (to prevent mold generated in the sugar solution and to remove viruses, etc.) Therefore, a sugar solution (including a mixture of 0.1 cc of nanosilver-supported collagen water with 100 cc of water) and undried fresh pollen or kneaded pollen with the fresh pollen placed on the cap And keep it for about 2 days while giving it.

  After about two days, the new queen bee is accommodated again in the closed space of the container, and carbon dioxide is poured into the closed space following the same procedure as the previous time. The supply of carbon dioxide in this case is performed until the new queen bee housed in the same manner as before stops its movement and becomes anesthetized, and is left in the atmosphere for 30 to 70 minutes. . After the elapse of the standing time, the container is opened, the new queen bee is taken out from the closed space, returned to the compartment 12 of the gauge 11, and the sleep process for the new queen bee is completed.

  For this reason, according to the third method, a unique nap treatment with a low mortality rate using carbon dioxide is performed twice on the new Japanese queen bee of Japanese black bee that has finished mating with the Japanese bee of Japanese black bee. The new queen bee can avoid spawning and start spawning.

  Incidentally, under the above conditions, anesthesia treatment (dormant treatment) was performed on 228 Japanese native bee by the third method under the above conditions, and for 209 eggs (91.67%) that laid eggs, The number of individuals that did not lay eggs was 19 (8.33%). The number of individuals that formed colonies (30 or more worker bees) was 165 (72.37%), whereas the number of individuals that did not form colonies was 63 (27.63%).

  In the fifth step, a plurality of new queen bees that have finished sleeping after the fourth step are competingly raised in the cage 11. Specifically, after flying each new queen bee in the compartment 12 of the cage 11 for 3 days, the bumble bees and pseudo moths of bumble bees native to Japan are put in a state of being fixed to the board pieces with wax, and A plurality of new queen bees are also put in a companion breeding in the compartment 12 of the cage 11, thereby creating a competitive relationship with each other to promote spawning and increase efficiency.

  In the sixth step, when the new queen bee that has passed through the fifth step starts laying eggs, each new queen bee is moved into the nesting chamber 23 of the small box 15 of FIG. 2 maintained at an internal temperature of about 27 degrees Celsius. Return to the first step and repeat this.

  For this reason, according to the present invention, it is possible to lay eggs in a queen bee of Japanese native bumblebees, and to apply a dormancy treatment to a new queen bee after maturing with a male bee of Japanese native bumblebees to avoid overwintering it can. For this reason, Japanese native bumblebees can be reared systematically while realizing generational changes with a cycle shorter than one year.

  Therefore, in the cultivation facility of solanaceous plants such as tomatoes, the Japanese native bumblebees should be used as a pollinator for artificial pollination by installing a nest box formed with colonies made of Japanese native bumblebees. Can do. Moreover, since the bumblebees used are native to Japan, even if they escape from the facility, there is no risk of serious adverse effects on the Japanese ecosystem.

  It has been confirmed that the dormancy process (fourth step) according to the present invention is effective when it is performed again when it is difficult to lay eggs in the fifth step or when the nest is abandoned on the way. In that case, it is necessary to reduce the processing conditions in any sleep processing. Specifically, in the case of the first method (low temperature processing method), the time for exposure to low temperature is set in a short time. In the case of the second method (water treatment method), the time for submerging is shortened and the light asphyxia is set. In the case of the third method (anesthetic treatment), the treatment time is shortened by about 5 to 15 minutes.

  Further, in the dormancy process (fourth step) in the present invention, as a method for avoiding the overwintering among the first technique (low temperature treatment method), the second technique (water treatment method) and the third technique (anesthetic treatment). By adopting an appropriate one at random, it is possible to avoid the inconvenience that the sensitivity, which tends to occur in an individual, becomes weaker or less effective.

  Further, it is preferable that all the small boxes 15 and the large boxes 31 are placed with sodium chloride in a small container such as a cap of a plastic bottle. In addition, it is preferable to put bone charcoal in all the small boxes 15 and the large boxes 31. In this case, in the small box 15, one cup of a PET bottle cap is contained in the large box 31. It is preferable to store 3 to 4 cups of PET bottle caps. By doing so, it is possible to achieve humidity adjustment in the box and prevention of mold generation.

  The Japanese bumblebees to which the present invention is applied include black bee, bumble bee, Miyama bumble bee, bumble bee, Ezo bumble bee, Ezo bumble bee, tramal bee and the like.

The whole perspective view which shows an example of the gauge made from the net used when putting a new queen bee in the middle of a dormancy process temporarily, flying a new queen bee after mating, or encouraging egg-laying. The schematic perspective view which shows an example of the small box used when obtaining a worker bee from the queen bee of Japanese black bee or making a colony. The schematic perspective view which shows typically an example of the large box which transfers a colony when the worker bee in a small box becomes around ten heads.

Explanation of symbols

DESCRIPTION OF SYMBOLS 11 Cage 12 Compartment 15 Small box 16 Box main body 16a Vent hole 17 Opening surface 18 Bottom plate 19 Side plate 20 Side plate 20a Notch 21 Nail material 22 Partition plate 22a Notch 23 Nesting chamber 24 Feeding chamber 25 Opening 26 Feeder 26a Insertion 27 Net material 28 Transparent top plate 28a Right top plate portion 28b Left top plate portion 29 Hinge 31 Large box 31a Bottom surface 32 Top plate 33 Partition plate 33a Through hole 34 Nesting chamber 35 Feeding chamber 36 Colony 37 Gum tape 38 Side plate 39 Insertion hole 40 Pollen 41 Feeder 42 Net material 43 Sucrose solution 44 Board piece

Claims (6)

A honey bee queen bee that has finished mating with a Japanese bee bumble bee is placed in the nesting room of a small box having a nesting room and a feeding room that are freely detachable, and a worker bee is obtained by laying eggs. The first step;
When the number of worker bees reaches about 10 in the breeding room of a large box having a nesting room and a feeding room partitioned so as to be freely accessible, the colonies are transferred to each colony under a temperature state of 23 degrees Celsius or more. The second step of bringing out the pupae and raising them as bees,
A third step of taking out and mating a new queen bee and a male bee from the bee group raised in the second step;
A fourth step of applying dormancy treatment to the new queen bee after mating;
A fifth step of competingly breeding a plurality of new queen bees in the cage after having undergone the fourth step;
When the new queen bee that has passed through the fifth step starts laying eggs, each new queen bee is transferred into the nesting chamber of the small box maintained at a temperature of about 27 ° C. and is returned to the first step. And a breeding / feeding / raising method of Japanese native bumblebees characterized by comprising: The method for breeding and feeding Japanese native bumblebees according to claim 1, wherein in the first step, nesting is promoted by fixing a bumble bee of Japanese bumblebees with wax in the nesting room. The method for breeding and feeding Japanese bumblebees according to claim 1 or 2, wherein a bottom surface of the large box is formed of a net material. In the fourth step, the dormancy treatment is performed by adding a 10% bone charcoal to a soil obtained by mixing 30% of multifunctional bio soil with 70% of the breeding soil and humus soil at a ratio of 1: 1. A pre-treated soil made by spraying 6 ppm nano-silver-bearing collagen water on the mixture and laying it in a closed space to a thickness of 1 to 2 cm, and then receiving the new queen bee that has undergone the third step; ,
A process of allowing the queen bee after being housed in the closed space to sleep in a low temperature state of 8 to 10 ° C. for about 1 to 3 weeks;
4. Breeding and breeding of Japanese native bumblebees according to any one of claims 1 to 3, wherein the queen bee after dormancy is taken out, and the temperature is gradually raised and finally treated to 25 to 28 ° C. Method. The dormancy treatment in the fourth step is a temporary death while observing the individual difference of the new queen bee that has undergone the third step in water of 5 to 30 ° C. in which 0.1 cc of 6 ppm nanosilver-carrying collagen water is mixed with 100 cc of water. A process of submerging until it reaches a state,
Removing water adhering to the body surface after taking it out of the water;
Immediately after removing the water, put it in a closed space of 25-30 ° C., give the sugar solution and pollen to which nano silver-supporting collagen as a feed is added, and fly while repeating the above treatment including submersion until the next treatment The first feeding flight process,
The breeding / feeding / raising method of Japanese native bumblebees according to any one of claims 1 to 3, wherein the second feeding / flying process is performed through at least a second feeding / flying process in which the same process as the first feeding / flying process is performed within one week. The dormancy process in the fourth step is a process of accommodating the new queen bee that has undergone the third step in a closed space;
A process of supplying carbon dioxide gas into the closed space until the new queen bees stop moving and becoming anesthetized, leaving it in the atmosphere for 30 to 70 minutes, taking it out and raising it for several days; ,
After that, the queen bee is accommodated again in the closed space, and carbon dioxide gas is supplied into the closed space until the queen bee stops its movement and becomes anesthetized. The method for breeding and feeding Japanese native bumblebees according to any one of claims 1 to 3, wherein the treatment is carried out through at least the process of leaving after standing for 45 minutes.

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