CN104996366A - Trichogramma japonicum high-efficiency reproduction method - Google Patents
Trichogramma japonicum high-efficiency reproduction method Download PDFInfo
- Publication number
- CN104996366A CN104996366A CN201510470852.1A CN201510470852A CN104996366A CN 104996366 A CN104996366 A CN 104996366A CN 201510470852 A CN201510470852 A CN 201510470852A CN 104996366 A CN104996366 A CN 104996366A
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- CN
- China
- Prior art keywords
- trichogramma
- rice
- card
- egg cards
- egg
- Prior art date
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- 238000000034 method Methods 0.000 title claims abstract description 20
- 241001238248 Trichogramma japonicum Species 0.000 title abstract 4
- 241000256844 Apis mellifera Species 0.000 claims abstract description 37
- 241000753145 Sitotroga cerealella Species 0.000 claims abstract description 20
- 230000003071 parasitic effect Effects 0.000 claims abstract description 18
- 235000015097 nutrients Nutrition 0.000 claims abstract description 12
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 11
- 229930006000 Sucrose Natural products 0.000 claims abstract description 11
- 102000002322 Egg Proteins Human genes 0.000 claims description 37
- 108010000912 Egg Proteins Proteins 0.000 claims description 37
- 210000004681 ovum Anatomy 0.000 claims description 37
- 241000256618 Trichogramma Species 0.000 claims description 25
- 230000024241 parasitism Effects 0.000 claims description 10
- 239000005720 sucrose Substances 0.000 claims description 10
- 238000009395 breeding Methods 0.000 claims description 6
- 230000001488 breeding effect Effects 0.000 claims description 6
- 239000007921 spray Substances 0.000 claims description 6
- 230000032669 eclosion Effects 0.000 claims description 3
- 210000004894 snout Anatomy 0.000 claims 2
- 241000426497 Chilo suppressalis Species 0.000 abstract description 5
- 239000000575 pesticide Substances 0.000 abstract description 5
- 235000013601 eggs Nutrition 0.000 abstract 11
- 241000098289 Cnaphalocrocis medinalis Species 0.000 abstract 1
- 239000011087 paperboard Substances 0.000 abstract 1
- 229960004793 sucrose Drugs 0.000 abstract 1
- 241000209094 Oryza Species 0.000 description 10
- 235000007164 Oryza sativa Nutrition 0.000 description 10
- 235000009566 rice Nutrition 0.000 description 10
- 238000005516 engineering process Methods 0.000 description 6
- 241000008892 Cnaphalocrocis patnalis Species 0.000 description 4
- 241000607479 Yersinia pestis Species 0.000 description 4
- 230000002265 prevention Effects 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 241000238631 Hexapoda Species 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 238000005286 illumination Methods 0.000 description 3
- 244000045947 parasite Species 0.000 description 3
- 239000003905 agrochemical Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000003449 preventive effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241001415070 Arctiinae Species 0.000 description 1
- 241000122049 Hesperiidae Species 0.000 description 1
- 241000257303 Hymenoptera Species 0.000 description 1
- 241001124569 Lycaenidae Species 0.000 description 1
- 241000256259 Noctuidae Species 0.000 description 1
- YBZUGUWOQLUNKD-PMPSAXMXSA-N Palustrine Chemical compound C1C(=O)NCCCCNCCCN2[C@H]([C@@H](O)CC)CC=C[C@@H]21 YBZUGUWOQLUNKD-PMPSAXMXSA-N 0.000 description 1
- YMALJVLSPODBKM-UHFFFAOYSA-N Palustrine Natural products C1C(=O)NCCCCNCCCN2C(C(O)CC)C=CCC21 YMALJVLSPODBKM-UHFFFAOYSA-N 0.000 description 1
- 241001622642 Parnara bada Species 0.000 description 1
- 241000255893 Pyralidae Species 0.000 description 1
- 241000256680 Trichogrammatidae Species 0.000 description 1
- 230000009418 agronomic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000443 biocontrol Effects 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 238000009329 organic farming Methods 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Provided is a trichogramma japonicum high-efficiency reproduction method. The method comprises: collecting eggs of rice moths, and sticking the eggs on paper boards to make egg cards; putting trichogramma japonicum species and eggs of rice moths in a darkroom in proportion of 1:10 to 1:4, adjusting bee species emergence parasitic environment temperature to 23 to 25 DEG C, adjusting humidity to 70% to 80%; after 90% of the egg cards have parasitic bee species, adding fresh rice moth egg cards according to 1:6-10 of proportion of the bee species and the egg cards again, properly increasing temperature of the darkroom to 25 to 28 DEG C, and maintaining the humidity; after more than 90% of the egg cards have parasitic bee species, adding fresh rice moth egg cards according to 1:3-7 of proportion of the bee species and the egg cards for the third time, the egg cards this time being sprayed with cane sugar nutrient solution, the bee species continuing to reproduce. The method can realize using the trichogramma japonicum to prevent rice leaf folders and striped rice borers, and pesticide dose is greatly reduced. The method promotes agriculture to develop towards green, organic, and ecological agriculture.
Description
Technical field
The invention belongs to rice disease and pest technical field of biological control, be specifically related to a kind of Trichogramma efficient propagation method.
Background technology
Trichogramma belongs to Hymenoptera, Trichogrammatidae, hobby moves in rice field or similar palustrine, can the worm's ovum of some insects such as parasitic Pyralidae, Noctuidae, Hesperiidae, little Lycaenidae, Arctiidae, also be simultaneously the ovum phase natural enemy of the Insect Pests in Rices such as striped rice borer, rice leaf roller, rice green caterpillar, rice plant skipper, to rice pest, there is certain preventive and therapeutic effect.
The Ministry of Agriculture proposes: to the year two thousand twenty, China's agricultural will realize " control two subtracts three basic ", agricultural chemicals aspect, promote diseases and pests of agronomic crop Unifying control and green prevention and control, preferential employing Ecological Control, physical control and bio-control method, carry out the Demonstration And Extension of low toxicity and low residue agricultural chemicals, to the year two thousand twenty, staple crops damage by disease and insect green prevention and control coverage rate reaches 30%, and chemical pesticide usage amount realizes zero growth rate.In recent years, utilize Trichogramma control striped rice borer and rice leaf roller green prevention and control technology successfully to demonstrate in South Rice Region of China large area such as China Hunan, Jiangxi, Sichuan, Guangxi, greatly reduce the use of chemical pesticide.Hunan Rice Research Institute's research shows, the large area control effect of Trichogramma to rice leaf roller and striped rice borer reach respectively 69.34% ?87.10% and 69.61% ?77.29%, compared with chemical pesticide, preventive effect is suitable.
Along with this green prevention and control technology constantly expands at South Rice Region of China promoted extension, increasing to the demand of Trichogramma, existing oophagous trichogrammae propagation technique and efficiency have been difficult to meet current market demand, seriously govern that this technology is more large-area to be applied.The art of this patent is on the basis that forefathers oophagous trichogrammae cultivates, in conjunction with the physiological growth characteristic of Trichogramma, propose the in batches parasitic method being suitable for the parasitic dark surrounds of Trichogramma breeding, improving reproduction coefficient, and improve the nutrient solution of bee hatching rate and eclosion rate, effectively improve reproduction coefficient and the honeybee kind quality of Trichogramma, the large scale application that can be rice green culture technique is taken a firm foundation.
Summary of the invention
Technical problem to be solved by this invention is: the problem used in a large number for chemical agent in south rice production process, and provide a kind of Trichogramma efficient propagation method, the object of Trichogramma control rice leaf roller and striped rice borer is realized to large area, significantly reduce South Rice Region of China chemical pesticide consumption, promote the development of China's agricultural to green agriculture, organic farming, the ecological agriculture.
The embodiment of this technology is: collect rice moth egg and to be sticked on cardboard and to make ovum card; Trichogramma kind honeybee and rice moth egg card are placed in darkroom in the ratio of 1:10 ~ 14, and kind of a bee eclosion parasitic environment temperature is adjusted to 23 ?25 DEG C, humidity 70% ?80%; After ovum card more than 90% is by honeybee kind parasitism, again add fresh rice moth egg card according to the ratio of honeybee kind and ovum card 1:6 ~ 10, suitably raise darkroom temperature to 25 ?28 DEG C, humidity is constant; After ovum card more than 90% is by honeybee kind parasitism, adds fresh rice moth egg card according to the ratio third time of honeybee kind and ovum card 1:3 ~ 7, this deutovum card sprays sucrose nutrient solution, continues breeding.
In technique scheme, described sucrose nutrient solution is 5% sucrose nutrient solution.
Compared with prior art, beneficial effect of the present invention is:
First, Trichogramma can parasitic rice moth egg under illumination and dark two kinds of situations, prior art is normally parasitic under illumination condition, the present invention studies discovery by experiment, under dark condition, the life cycle of Trichogramma slightly extends, and is better than illumination condition to the parasitic effects of rice moth egg, significantly can improve the fertility of Trichogramma.
The second, fresh rice moth egg card divides by the method that quantity is successively decreased by the present invention allows for three times Trichogramma honeybee kind parasitic, and compared with existing disposable Parasite technology, point secondary parasite can ensure the freshness of rice moth egg, thus makes rice moth egg more parasitic.
3rd, the parasitic cycle of existing oophagous trichogrammae propagation technique is short, general 2 ?within 3 days, whole parasitic processes can be completed, therefore, the nutritional supplementation in parasitic processes does not come into one's own always.The present invention by parasitic cycle stretch-out, ensure that the parasitic ability of later stage Trichogramma by point secondary parasite, honeybee card and ovum card sprays 5% sucrose nutrient solution, can improve the vigor of Trichogramma, thus improve the reproduction coefficient of Trichogramma further.
Test effect:
According to upper table, the result of the test on May 14th, 2015 and June 4 shows, compared with the existing propagation technique of Trichogramma, the present invention effectively can improve the reproduction coefficient of Trichogramma, and reproduction coefficient reaches as high as 1:27.9.
Embodiment
Embodiment 1:
Collect rice moth egg and make ovum card; Trichogramma honeybee kind and ovum card are placed in darkroom in the ratio of 1:12, and darkroom temperature is adjusted to 23 DEG C, humidity 70%; After ovum card more than 90% is by honeybee kind parasitism, add fresh ovum card according to the ratio of honeybee kind and ovum card 1:8, suitably raise darkroom temperature to 25 DEG C, humidity is constant; After ovum card more than 90% is by honeybee kind parasitism, again add fresh ovum card according to the ratio of honeybee kind and ovum card 1:5, this deutovum card sprays 5% sucrose nutrient solution, continues breeding.
Embodiment 2:
Collect rice moth egg and make ovum card; Trichogramma honeybee kind and ovum card are placed in darkroom in the ratio of 1:10, and darkroom temperature is adjusted to 24 DEG C, humidity 75%; After ovum card more than 90% is by honeybee kind parasitism, add fresh ovum card according to the ratio of honeybee kind and ovum card 1:6, suitably raise darkroom temperature to 26 DEG C, humidity is constant; After ovum card more than 90% is by honeybee kind parasitism, again add fresh ovum card according to the ratio of honeybee kind and ovum card 1:3, this deutovum card sprays 5% sucrose nutrient solution, continues breeding.
Embodiment 3:
Collect rice moth egg and make ovum card; Trichogramma honeybee kind and ovum card are placed in darkroom in the ratio of 1:14, and darkroom temperature is adjusted to 25 DEG C, humidity 80%; After ovum card more than 90% is by honeybee kind parasitism, add fresh ovum card according to the ratio of honeybee kind and ovum card 1:10, suitably raise darkroom temperature to 28 DEG C, humidity is constant; After ovum card more than 90% is by honeybee kind parasitism, again add fresh ovum card according to the ratio of honeybee kind and ovum card 1:7, this deutovum card sprays 5% sucrose nutrient solution, continues breeding.
Claims (2)
1. a snout moth's larva oophagous trichogrammae efficient propagation method, is characterized in that, collects rice moth egg and to be sticked on cardboard and to make ovum card; Trichogramma kind honeybee and rice moth egg card are placed in darkroom in the ratio of 1:10 ~ 14, and kind of a bee eclosion parasitic environment temperature is adjusted to 23 ?25 DEG C, humidity 70% ?80%; After ovum card more than 90% is by honeybee kind parasitism, again add fresh rice moth egg card according to the ratio of honeybee kind and ovum card 1:6 ~ 10, suitably raise darkroom temperature to 25 ?28 DEG C, humidity is constant; After ovum card more than 90% is by honeybee kind parasitism, adds fresh rice moth egg card according to the ratio third time of honeybee kind and ovum card 1:3 ~ 7, this deutovum card sprays sucrose nutrient solution, continues breeding.
2. snout moth's larva oophagous trichogrammae efficient propagation method according to claim 1, is characterized in that, described sucrose nutrient solution is 5% sucrose nutrient solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510470852.1A CN104996366B (en) | 2015-08-04 | 2015-08-04 | A kind of Trichogramma efficient propagation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510470852.1A CN104996366B (en) | 2015-08-04 | 2015-08-04 | A kind of Trichogramma efficient propagation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104996366A true CN104996366A (en) | 2015-10-28 |
| CN104996366B CN104996366B (en) | 2017-07-14 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510470852.1A Active CN104996366B (en) | 2015-08-04 | 2015-08-04 | A kind of Trichogramma efficient propagation method |
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| CN (1) | CN104996366B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106689063A (en) * | 2016-12-07 | 2017-05-24 | 中国科学院动物研究所 | Artificial breeding production method of trichogramma japonicum |
| CN108056070A (en) * | 2018-01-16 | 2018-05-22 | 吉林农业大学 | Utilize the method for white snow moths attracted by lamplight breeding trichogramma |
| CN111011313A (en) * | 2019-12-30 | 2020-04-17 | 中国医学科学院药用植物研究所云南分所 | Method for improving female proportion of trichogramma offspring and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1647626A (en) * | 2005-01-26 | 2005-08-03 | 北京市农林科学院 | Artificial breeding and producing method for trichogramma |
| CN101438691A (en) * | 2007-11-23 | 2009-05-27 | 北京市农林科学院 | Mass propagation method of Oophagous trichogrammae from artificial ovum |
| CN102106319A (en) * | 2011-01-18 | 2011-06-29 | 西北农林科技大学 | Method for propagating trichogramma pintoi voegele indoors by grapholitha molesta busck egg |
-
2015
- 2015-08-04 CN CN201510470852.1A patent/CN104996366B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1647626A (en) * | 2005-01-26 | 2005-08-03 | 北京市农林科学院 | Artificial breeding and producing method for trichogramma |
| CN101438691A (en) * | 2007-11-23 | 2009-05-27 | 北京市农林科学院 | Mass propagation method of Oophagous trichogrammae from artificial ovum |
| CN102106319A (en) * | 2011-01-18 | 2011-06-29 | 西北农林科技大学 | Method for propagating trichogramma pintoi voegele indoors by grapholitha molesta busck egg |
Non-Patent Citations (2)
| Title |
|---|
| 王卫华 等: ""中间寄主对甘蓝夜蛾赤眼蜂寄生行为的影响"", 《植物保护》 * |
| 陈红印 等: ""以米蛾卵为寄主繁殖玉米螟赤眼蜂的质量控制技术"", 《昆虫天敌》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106689063A (en) * | 2016-12-07 | 2017-05-24 | 中国科学院动物研究所 | Artificial breeding production method of trichogramma japonicum |
| CN108056070A (en) * | 2018-01-16 | 2018-05-22 | 吉林农业大学 | Utilize the method for white snow moths attracted by lamplight breeding trichogramma |
| CN111011313A (en) * | 2019-12-30 | 2020-04-17 | 中国医学科学院药用植物研究所云南分所 | Method for improving female proportion of trichogramma offspring and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104996366B (en) | 2017-07-14 |
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