CN102106319A - Method for propagating trichogramma pintoi voegele indoors by grapholitha molesta busck egg - Google Patents
Method for propagating trichogramma pintoi voegele indoors by grapholitha molesta busck egg Download PDFInfo
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- CN102106319A CN102106319A CN 201110009934 CN201110009934A CN102106319A CN 102106319 A CN102106319 A CN 102106319A CN 201110009934 CN201110009934 CN 201110009934 CN 201110009934 A CN201110009934 A CN 201110009934A CN 102106319 A CN102106319 A CN 102106319A
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- fruit moth
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- oriental fruit
- oophagous trichogrammae
- black dull
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Abstract
The invention provides a method for propagating the trichogramma pintoi voegele indoors by the grapholitha molesta busck egg. The method comprises the steps of: taking the grapholitha molesta busck egg as the host of a parasite, and continuously propagating the trichogramma pintoi voegele generation by generation under the laboratory conditions. The grapholitha molesta busck egg is taken as the host of the parasite, the artificial propagation of the trichogramma pintoi voegele is not limited by the time and the space and can be continuously propagated by means of subculture, the trichogramma pintoi voegele propagating quality is high, the parasitic rate is stable, and the method has various advantages, so that the method offers a new approach for preventing the trichogramma pintoi voegele from being propagated in quantity in the field of the biological prevention of the farming and forestry pests.
Description
Technical field
This research relates to the insect egg parasitoids---the artificial fecundation method of black dull oophagous trichogrammae, particularly a kind of biological control technical field of utilizing the method for breeding black dull oophagous trichogrammae in the oriental fruit moth ooecium to belong to pest.
Background technology
Black dull oophagous trichogrammae Trichogramma pintoi Voegele is the insect egg parasitoids---oophagous trichogrammae a kind of, it is the natural enemy that a class has the value utilized very much, being distributed widely in countries in the world, is one of egg parasitoids honeybee kind of studying at present and be most widely used in the biological control of insect pests in the world wide.Utilize the oophagous trichogrammae pest control, not only reduced the use of the chemical pesticide in the control of insect practice, reduced chemical pesticide residual in agricultural product, and have an important practice significance for protection environment and the bio-diversity that improves in the natural environment.
In the numerous bee technology of the existing oophagous trichogrammae of China, mainly use large-scale ovum to do vector, as egg of oak silkworm.Its advantage is that bee yield is big, economy, but numerous honeybee kind the control efficiency of target pest is often had very big difference.In recent years, people in order to improve numerous honeybee kind the control efficiency of target pest is attempted with small-sized ovum as the patent application of patent publication No. CN1397159A, to utilize the technology of industrially culturing trichogramma with gelechiid ova as the vector breeding oophagous trichogrammae exactly.
Oriental fruit moth Grapholitha molesta Busck is a kind of global fruit tree pest insect, blazons Asia, Europe, America and Australia.China is not except that appearing in the newspapers in Tibet, and other each province (city, district) all has generation, and the past takes place general especially with North China, Central China, and harm is serious.But in recent years, with the adjustment of the industrial structure, very big variation has taken place in the tillage and cultivation system, provinces and regions such as northwest Shaanxi, Gansu, and fruit had become the mainstay industry of local agricultural production already, and the generation harm of oriental fruit moth obviously increases the weight of.
Summary of the invention
The purpose of present technique provides a kind of method of breeding black dull oophagous trichogrammae with the oriental fruit moth ovum.Utilize the technology of the black dull oophagous trichogrammae of a large amount of breedings of oriental fruit moth ovum, may further comprise the steps:
(1) collection of oriental fruit moth ovum and processing
With product in the oriental fruit moth 24h after the ovum on the template takes out together with template, blow away the scale on the template gently, with the cotton balls that speckles with 75% ethanol wiping paper gently, the ultraviolet ray that is put in light intensity 21.2Lux then is irradiation 2h down, to kill the embryo in the oriental fruit moth ovum.
(2) preservation of oriental fruit moth ovum
To produce on template, and bind up with gauze together with template through the oriental fruit moth ovum of UV treatment and be put in lid and be drilled with in the round plastic lunch box of aperture, and place the moistening cotton balls of a finger size and preserve moisture, in 4 ℃ of refrigerators, preserve.
(3) breeding of black dull oophagous trichogrammae
A. the oriental fruit moth ovum that will preserve in advance takes out from refrigerator puts into 2cm * 15cm test tube, each test tube is put 300 approximately, inserted black dull oophagous trichogrammae queen bee than 1: 30 by the honeybee ovum, the absorbent cotton cotton thread that is about 2cm, dips in 5% hydromel is sticked in vitro tube wall apart from the about 2cm of mouth of pipe place, as the extra-nutrition source of black dull oophagous trichogrammae adult.
B. seal with the degreasing tampon, put 26 ℃ of temperature, humidity RH 60% ± 10%, cultivates in the dark fully climatic cabinate.Behind the 48h, become honeybee to shift out black dull oophagous trichogrammae, the oriental fruit moth ovum continues to cultivate in climate box.
C. checking after cultivating 5d in the climatic cabinate, become black by the oriental fruit moth ovum of black dull oophagous trichogrammae parasitism, still is not milky by the ovum of parasitism.
D. after continuing to cultivate 10d, black dull oophagous trichogrammae of new generation becomes honeybee to begin to sprout wings successively, can carry out subculture and raise.
The present invention can successfully raise on the basis of oriental fruit moth indoor, for new way and the new method of seeking the oriental fruit moth biological control, utilize the oriental fruit moth ovum to breed black dull oophagous trichogrammae, the result shows, black dull oophagous trichogrammae can be the host with the oriental fruit moth ovum fully, successfully finish ontogeny and successive propagation, this lays a good foundation for utilizing black dull oophagous trichogrammae control oriental fruit moth, has the potential value of large-scale promotion.
The advantage of present technique is, adopts the oriental fruit moth ovum as vector, and artificial propagation is not subjected to the restriction of time and region, and numerous honeybee kind is many, but extensive use, and also the performance of numerous honeybee quality is obviously, and parasitic rate and eclosion rate are higher, demonstrate multiple advantage.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in detail.
Present technique utilizes the oriental fruit moth ovum to breed black dull oophagous trichogrammae, comprises following technical process:
(1) collection of oriental fruit moth ovum and processing
With product in the oriental fruit moth 24h after the ovum on the template takes out together with template, blow away the scale on the template gently, with the cotton balls that speckles with 75% ethanol wiping paper gently, the ultraviolet ray that is put in light intensity 21.2Lux then is irradiation 2h down, to kill the embryo in the oriental fruit moth ovum.
(2) preservation of oriental fruit moth ovum
To produce on template, and bind up with gauze together with template through the oriental fruit moth ovum of UV treatment and be put in lid and be drilled with in the round plastic lunch box of aperture, and place the moistening cotton balls of a finger size and preserve moisture, in 4 ℃ of refrigerators, preserve.
(3) breeding of black dull oophagous trichogrammae
A. the oriental fruit moth ovum that will preserve in advance takes out from refrigerator puts into 2cm * 15cm test tube, each test tube is put 300 approximately, inserted black dull oophagous trichogrammae queen bee than 1: 30 by the honeybee ovum, the absorbent cotton cotton thread that is about 2cm, dips in 5% hydromel is sticked in vitro tube wall apart from the about 2cm of mouth of pipe place, as the extra-nutrition source of black dull oophagous trichogrammae adult.
B. seal with the degreasing tampon, put 26 ℃ of temperature, humidity RH 60% ± 10%, cultivates in the dark fully climatic cabinate.Behind the 48h, become honeybee to shift out black dull oophagous trichogrammae, the oriental fruit moth ovum continues to cultivate in climate box.
C. checking after cultivating 5d in the climatic cabinate, become black by the oriental fruit moth ovum of black dull oophagous trichogrammae parasitism, still is not milky by the ovum of parasitism.
D. after continuing to cultivate 10d, black dull oophagous trichogrammae of new generation becomes honeybee to begin to sprout wings successively, can carry out subculture and raise.
(4) evaluation of reproductive effect
The applicant uses method of the present invention in 15 generations of the black dull oophagous trichogrammae of indoor continuous breeding, measures the main life parameters of each worm attitude respectively in the 1st, 5,10,15 generations, and the result shows that the life parameters difference between each from generation to generation is very little, and population development is neat (seeing attached list) relatively.Illustrate that the employing method can satisfy the requirement that the indoor subculture of black dull oophagous trichogrammae is raised fully, and can promote aborning, by a large amount of breeding control oriental fruit moth.But from subordinate list also as can be seen, reported that with other oophagous trichogrammae indoors artificial raising technology is the same, along with the increase of propagation generation number, the degradation phenomena of black dull oophagous trichogrammae also clearly.So suggestion utilizes the oriental fruit moth ovum to breed about 10 generations in actual use, the ovum of using egg of oak silkworm or other hosts carries out the rejuvenation of honeybee kind.
The indoor effect detection result who utilizes the oriental fruit moth ovum to breed black dull oophagous trichogrammae of subordinate list
Should be understood that, for those of ordinary skills, can be improved according to the above description or conversion, and all these improvement and conversion all should belong to the protection domain of claims of the present invention.
Claims (4)
1. a method of utilizing the black dull oophagous trichogrammae of breeding in the oriental fruit moth ooecium is characterized in that, may further comprise the steps:
(1) collection of oriental fruit moth ovum and processing;
(2) preservation of oriental fruit moth ovum; To produce on template, and bind up with gauze together with template through the oriental fruit moth ovum of UV treatment and be put in lid and be drilled with in the round plastic lunch box of aperture, and place the moistening cotton balls of a finger size and preserve moisture, in 4 ℃ of refrigerators, preserve;
(3) breeding of black dull oophagous trichogrammae:
(31) the oriental fruit moth ovum that will preserve in advance takes out from refrigerator puts into 2cm * 15cm test tube, and each test tube is put 300 approximately, inserts black dull oophagous trichogrammae queen bee than 1: 30 by the honeybee ovum;
(32) seal with the degreasing tampon, put 26 ℃ of temperature, humidity RH 60% ± 10%, cultivates in the dark fully climatic cabinate;
(33) behind the cultivation 10d, black dull oophagous trichogrammae of new generation becomes honeybee to begin to sprout wings successively, can carry out subculture and raise.
2. the method for claim 1, it is characterized in that, described step (1): with product in the oriental fruit moth 24h after the ovum on the template takes out together with template, blow away the scale on the template gently, with the cotton balls that speckles with 75% ethanol wiping paper gently, 2h is shone in the ultraviolet ray that is put in light intensity 21.2Lux then down, to kill the embryo in the oriental fruit moth ovum.
3. the method for claim 1, it is characterized in that, after described step (31) inserts black dull oophagous trichogrammae queen bee, the absorbent cotton cotton thread that is about 2cm, dips in 5% hydromel is sticked in vitro tube wall apart from the about 2cm of mouth of pipe place, as the extra-nutrition source of black dull oophagous trichogrammae adult.
4. the method for claim 1 is characterized in that, described step (32) behind the cultivation 48h, shifts out black dull oophagous trichogrammae queen bee, and the oriental fruit moth ovum continues to cultivate in climate box; Checking after cultivating 5d in the climatic cabinate, become black by the oriental fruit moth ovum of black dull oophagous trichogrammae parasitism, still is not milky by the ovum of parasitism.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100099348A CN102106319B (en) | 2011-01-18 | 2011-01-18 | Method for propagating trichogramma pintoi voegele indoors by grapholitha molesta busck egg |
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| CN2011100099348A CN102106319B (en) | 2011-01-18 | 2011-01-18 | Method for propagating trichogramma pintoi voegele indoors by grapholitha molesta busck egg |
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| CN102106319A true CN102106319A (en) | 2011-06-29 |
| CN102106319B CN102106319B (en) | 2012-06-27 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103782969A (en) * | 2014-03-03 | 2014-05-14 | 广西壮族自治区林业科学研究院 | Method for breeding trichogramma pintoi indoors and corcyra cephalonica raising fodder |
| CN103918613A (en) * | 2014-04-23 | 2014-07-16 | 安徽师范大学 | Pupal-stage parasitic wasp artificial-propagation method, wasp-keeping box and manufacturing method of wasp-keeping box |
| CN104996366A (en) * | 2015-08-04 | 2015-10-28 | 湖南省水稻研究所 | Trichogramma japonicum high-efficiency reproduction method |
| CN111011313A (en) * | 2019-12-30 | 2020-04-17 | 中国医学科学院药用植物研究所云南分所 | Method for improving female proportion of trichogramma offspring and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1397159A (en) * | 2002-09-04 | 2003-02-19 | 衡水田益生防有限责任公司 | Technology for industrially culturing trichogramma with gelechiid ova |
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2011
- 2011-01-18 CN CN2011100099348A patent/CN102106319B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1397159A (en) * | 2002-09-04 | 2003-02-19 | 衡水田益生防有限责任公司 | Technology for industrially culturing trichogramma with gelechiid ova |
Non-Patent Citations (2)
| Title |
|---|
| 《昆虫学报》 19780302 山西省运城地区农科所 暗黑赤眼蜂生物学特征观察 第21卷, 第1期 2 * |
| 《赤眼蜂繁殖及田间应用技术》 20000831 中国农业科学院生物防治研究所组 第四章赤眼蜂寄主卵的生产与利用 , 1 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103782969A (en) * | 2014-03-03 | 2014-05-14 | 广西壮族自治区林业科学研究院 | Method for breeding trichogramma pintoi indoors and corcyra cephalonica raising fodder |
| CN103782969B (en) * | 2014-03-03 | 2015-08-19 | 广西壮族自治区林业科学研究院 | A kind of method of indoor propagation Trichogramma pintoi and rice moth culturing feed |
| CN103918613A (en) * | 2014-04-23 | 2014-07-16 | 安徽师范大学 | Pupal-stage parasitic wasp artificial-propagation method, wasp-keeping box and manufacturing method of wasp-keeping box |
| CN103918613B (en) * | 2014-04-23 | 2016-04-27 | 安徽师范大学 | A method for parasitic wasp artificial propagation in pupa time, and raise bees box and preparation method |
| CN104996366A (en) * | 2015-08-04 | 2015-10-28 | 湖南省水稻研究所 | Trichogramma japonicum high-efficiency reproduction method |
| CN104996366B (en) * | 2015-08-04 | 2017-07-14 | 湖南省水稻研究所 | A kind of Trichogramma efficient propagation method |
| CN111011313A (en) * | 2019-12-30 | 2020-04-17 | 中国医学科学院药用植物研究所云南分所 | Method for improving female proportion of trichogramma offspring and application thereof |
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| CN102106319B (en) | 2012-06-27 |
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