CN106376545A - Method for efficiently preserving Sclernderma guani - Google Patents
Method for efficiently preserving Sclernderma guani Download PDFInfo
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- CN106376545A CN106376545A CN201610831707.6A CN201610831707A CN106376545A CN 106376545 A CN106376545 A CN 106376545A CN 201610831707 A CN201610831707 A CN 201610831707A CN 106376545 A CN106376545 A CN 106376545A
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- 238000000034 method Methods 0.000 title claims abstract description 14
- 241000238631 Hexapoda Species 0.000 claims abstract description 5
- 206010039710 Scleroderma Diseases 0.000 claims description 31
- 241000256844 Apis mellifera Species 0.000 claims description 19
- 238000012856 packing Methods 0.000 claims description 17
- 238000004321 preservation Methods 0.000 claims description 17
- 241000254171 Curculionidae Species 0.000 claims description 9
- 210000000582 semen Anatomy 0.000 claims description 7
- 238000010792 warming Methods 0.000 claims description 7
- 241001147398 Ostrinia nubilalis Species 0.000 claims description 5
- 241000382353 Pupa Species 0.000 claims description 5
- 239000002917 insecticide Substances 0.000 claims description 5
- 241001609695 Anoplophora glabripennis Species 0.000 claims description 4
- 241001481710 Cerambycidae Species 0.000 claims description 4
- 241001609692 Anoplophora chinensis Species 0.000 claims description 2
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 2
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 2
- 241000541482 Curculio davidi Species 0.000 claims description 2
- 241000255777 Lepidoptera Species 0.000 claims description 2
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 2
- 241001035899 Xylotrechus grayii Species 0.000 claims description 2
- 239000011425 bamboo Substances 0.000 claims description 2
- 238000009395 breeding Methods 0.000 claims description 2
- 230000001488 breeding effect Effects 0.000 claims description 2
- 230000013011 mating Effects 0.000 claims description 2
- 238000012546 transfer Methods 0.000 claims description 2
- 241001525902 Sesiidae Species 0.000 claims 2
- 241000027431 Anoplophora Species 0.000 claims 1
- 241001330002 Bambuseae Species 0.000 claims 1
- 241000219000 Populus Species 0.000 claims 1
- 210000004894 snout Anatomy 0.000 claims 1
- 238000005286 illumination Methods 0.000 abstract description 6
- 230000004083 survival effect Effects 0.000 abstract description 5
- 241000257303 Hymenoptera Species 0.000 abstract description 3
- 238000003860 storage Methods 0.000 abstract description 3
- 238000001816 cooling Methods 0.000 abstract description 2
- 235000013601 eggs Nutrition 0.000 abstract description 2
- 230000007774 longterm Effects 0.000 abstract description 2
- 238000007710 freezing Methods 0.000 abstract 1
- 230000008014 freezing Effects 0.000 abstract 1
- 238000010438 heat treatment Methods 0.000 abstract 1
- 230000002035 prolonged effect Effects 0.000 abstract 1
- 230000019617 pupation Effects 0.000 abstract 1
- 230000032669 eclosion Effects 0.000 description 3
- 210000003141 lower extremity Anatomy 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241001124657 Bethylidae Species 0.000 description 1
- 241000254173 Coleoptera Species 0.000 description 1
- 241000590988 Danainae Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 244000082204 Phyllostachys viridis Species 0.000 description 1
- 241000255893 Pyralidae Species 0.000 description 1
- 241000467911 Sclerodermus guani Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0278—Physical preservation processes
- A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Husbandry (AREA)
- Animal Behavior & Ethology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a method for efficiently preserving Sclernderma guani, belonging to the technical field of insect cultivation. The method comprises the following steps: putting mated Sclernderma guani female bees on insect larvae or pupae in the early pupation period to lay eggs; after culture and cocooning, growing in a culture box at 20-30 DEG C under the humidity of 40-60% for 6-8 days; and cooling to 1-4 DEG C at the rate of 2-4 DEG C/hour, and preserving the bee cocoons for 120-360 days. When the Sclernderma guani is in use, the process comprises the following steps: heating the preserved bee cocoons to 8-12 DEG C at the rate of 2-4 DEG C/hour, and keeping the temperature for 4-6 hours; transferring the bee cocoons to natural illumination conditions or an artificial lighting envelopment with the illuminance of 200-500lux, and reviving at 20-30 DEG C under the humidity of 50-70% for 48-72 hours, thereby naturally emerging into Sclernderma guani adults. The cold storage time of the Sclernderma guani can be prolonged to 180-360 days at the low temperature of 1-4 DEG C in the form of cocoons, and the survival percent after long-term freezing can be ensured.
Description
Technical field
The invention belongs to cultured insect technical field.
Background technology
Scleroderma Xiao et Wu (Scleroderma guani Xiao et Wu), belongs to Hymenoptera, Bethylidae, is that one kind can be wide
The general Biological control type insecticide for preventing and treating Lepidoptera, coleoptera, Hymenoptera class pest, it has quite huge agricultural economy
Potentiality.The preservation of Scleroderma Xiao et Wu all the time is all that the honeybee that becomes after sprouting wings is carried out 3～10 DEG C of cold preservation, storage time with use
≤ 120 days, carried out putting honeybee using after front recovery.Existing document and research show that the one-tenth honeybee low temperature of Scleroderma Xiao et Wu stores pole
Limit is about 120 days, has no and the prior art indicate that the swollen lower limb honeybee of tubular type can store in the case of cold preservation cocoon.
Content of the invention
It is an object of the invention to provide a kind of method of efficient preservation Scleroderma Xiao et Wu, i.e. a kind of preservation Scleroderma Xiao et Wu
The method of cocoon, to extend the holding time of Scleroderma Xiao et Wu.
The inventive method is according to the following steps：
1st, by the Scleroderma Xiao et Wu queen bee of 2～3 days after successful mating, be placed into as host, a length of 10mm of body～
So as to lay eggs on the worm pupa at the insect larvae of 30mm or nymphosis initial stage；Described insecticide can be：The longicorn beetle of coleoptera Cerambycidae
(Anoplophora chinensis), anoplophora glabripennis (Anoplophora glabripennis), coffee borer
(Xylotrechus grayii Whit) etc.；The Semen Castaneae weevil (Curculio davidi Fairmaire) of Curculionidae, bamboo as
Worm (Otidognathus davidis Fair) etc.；The Pyrausta nubilalis (Hubern). (Ostrinia nubilalis) of Lepidoptera Pyralidae；Clearwing
Poplar clearwing moth (Parathrene tabaniformis [Rottenberg]) of moth section etc.；The preferred Semen Castaneae weevil of described insecticide.
2nd, will by the Host transfer of ovipositing hosts to cultivate box or raise pipe in, box or raise bottom of the tube be encased inside tissue paper or
Rice paper, stays conventinal breeding after air-vent, after Scleroderma Xiao et Wu is cocoond 8～24 hours, in humidity after the packing paper cocoond is collected
40%～60%, the culture box relaying supervention that 20 DEG C～30 DEG C of temperature educates 6～8 days.
3rd, the honeybee cocoon after the completion of previous step is cooled to 1～4 DEG C with the speed reducing by 2～4 DEG C per hour, at such a temperature
Preserve honeybee cocoon 120 days～360 days.
When the 4th, needing, the honeybee cocoon of previous step preservation is taken out, is warming up to 8～12 with the speed raising 2～4 DEG C per hour
DEG C, keep 4～6 hours；Then under the conditions of the tissue paper with cocoon being transferred to natural lighting or 200lux～500lux Manual light
Under luminous environment, recover 48～72 hours in the environment of 20 DEG C～30 DEG C of temperature, relative humidity 50%～70%, you can natural plumage
Turn to Scleroderma Xiao et Wu adult.
In the case of other conditions identical, using the store method of method for preserving of the present invention and traditional Scleroderma Xiao et Wu,
The survival rate comparative test result of Scleroderma Xiao et Wu is as follows：
| The preservation time | The survival rate of the inventive method | Tradition becomes honeybee refrigeration survival rate |
| 60 days | 92.3% | 84.3% |
| 90 days | 87.9% | 74.7% |
| 120 days | 80.4% | 14.6% |
| 180 days | 75.1% | 1.2% |
| 250 days | 57.9% | 0% |
| 300 days | 46.4% | 0% |
| 360 days | 33.2% | 0% |
Note：The host of contrast test is Semen Castaneae weevil larva.
The positive effect of the present invention：In a low temperature of 1 DEG C～4 DEG C use cocoon mode can by tubular type swell lower limb honeybee stored refrigerated when
Between increase to 180～360 days, improve the storage cycle of Scleroderma Xiao et Wu, and ensure Scleroderma Xiao et Wu after long-term frozen
Survival rate, greatly improves the production ability of regulation and control of Scleroderma Xiao et Wu.
Specific embodiment：
Embodiment 1：The transparent of bottom belt packing paper will be placed into by the pupa of the coffee borer of Scleroderma Xiao et Wu ovipositing hosts
After cellar culture is cocoond 8 hours to Scleroderma Xiao et Wu larva in culture box, take out the packing paper with cocoon and remove debris on packing paper, place
Continue culture 6 days in humidity 40%, the incubator of 30 DEG C of temperature, after taking-up, be cooled to 1 with 2 DEG C of the speed of lowering the temperature per hour
DEG C, it is transferred to 1 DEG C of constant temperature refrigerator and preserve 120 days.After the cocoon of cold preservation is taken out, it is warming up in one hour 4 at a temperature of 8 DEG C of holdings are somebody's turn to do
Hour；Be then transferred to environment be illumination 200lux, humidity 50%, culture 72 hours i.e. visible one-tenth honeybee plumage in 20 DEG C of environment of temperature
Change.
Embodiment 2：The transparent of bottom belt packing paper will be placed into by the Pyrausta nubilalis (Hubern). mature larva of Scleroderma Xiao et Wu ovipositing hosts
After cellar culture is cocoond 24 hours to Scleroderma Xiao et Wu larva in culture box, take out the packing paper with cocoon and remove debris on packing paper, put
Put humidity 60%, continue culture 8 days in the incubator of 20 DEG C of temperature, after taking-up, be cooled to 4 with 4 DEG C of the speed of lowering the temperature per hour
DEG C, it is transferred to 4 DEG C of constant temperature refrigerators and preserve 180 days.After the cocoon of cold preservation is taken out, be warming up in one hour 12 DEG C keep should at a temperature of
6 hours, be transferred to environment be illumination 500lux, humidity 70%, culture 48 hours i.e. visible one-tenth bee eclosion in 30 DEG C of environment of temperature.
Embodiment 3：The saturating of bottom belt packing paper will be placed into by the Semen Castaneae weevil mature larva of Scleroderma Xiao et Wu ovipositing hosts
After cellar culture is cocoond 20 hours to Scleroderma Xiao et Wu larva in bright culture box, take out the packing paper with cocoon and remove debris on packing paper,
It is placed into humidity 40%, continues culture 8 days in the incubator of 20 DEG C of temperature, with 4 DEG C of the speed cooling of lowering the temperature per hour after taking-up
To 1 DEG C, it is transferred to 1 DEG C of constant temperature refrigerator and preserves 360 days.After the cocoon of cold preservation is taken out, it is warming up to 8 DEG C in one hour and keeps this temperature
Lower 6 hours, be transferred to environment be illumination 200lux, humidity 50%, culture 48 hours i.e. visible one-tenth honeybee plumage in 30 DEG C of environment of temperature
Change.
Embodiment 4：The transparent culture of bottom belt packing paper will be placed into by the Semen Castaneae weevil pupa of Scleroderma Xiao et Wu ovipositing hosts
After cellar culture is cocoond 8 hours to Scleroderma Xiao et Wu larva in box, take out the packing paper with cocoon and remove debris on packing paper, be placed into wet
Degree 60%, continue culture 6 days in the incubator of 30 DEG C of temperature, be cooled to 4 DEG C with 2 DEG C of the speed of lowering the temperature per hour after taking-up, turn
Move to 4 DEG C of constant temperature refrigerators to preserve 280 days.After the cocoon of cold preservation is taken out, be warming up in one hour 12 DEG C keep should at a temperature of 4 little
When, be transferred to environment be illumination 200lux, humidity 50%, culture 72 hours i.e. visible one-tenth bee eclosion in 30 DEG C of environment of temperature.
Example 5：The transparent training of bottom belt packing paper will be placed into by the pupa of the anoplophora glabripennis of Scleroderma Xiao et Wu ovipositing hosts
After cellar culture is cocoond 18 hours to Scleroderma Xiao et Wu larva in foster box, take out the packing paper with cocoon and remove debris on packing paper, place
Continue culture 6 days in humidity 40%, the incubator of 30 DEG C of temperature, after taking-up, be cooled to 4 with 2 DEG C of the speed of lowering the temperature per hour
DEG C, it is transferred to 4 DEG C of constant temperature refrigerators and preserve 200 days.After the cocoon of cold preservation is taken out, it is warming up in one hour 4 at a temperature of 8 DEG C of holdings are somebody's turn to do
Hour, be transferred to environment be illumination 200lux, humidity 50%, culture 72 hours i.e. visible one-tenth bee eclosion in 20 DEG C of environment of temperature.
Claims (3)
1. a kind of method of efficient preservation Scleroderma Xiao et Wu is it is characterised in that according to the following steps：
(1), by the Scleroderma Xiao et Wu queen bee of 2～3 days after successful mating, it is placed into as host, a length of 10mm～30mm of body
Insect larvae or the worm pupa at nymphosis initial stage on so as to spawning；
(2), box will extremely be cultivated by the Host transfer of ovipositing hosts or raise in pipe, be encased inside tissue paper or a surname in box or raising bottom of the tube
Paper, stays conventinal breeding after air-vent, after Scleroderma Xiao et Wu is cocoond 8～24 hours, in humidity 40% after the packing paper cocoond is collected
～60%, the culture box relaying supervention that 20 DEG C～30 DEG C of temperature educates 6～8 days；
(3), the honeybee cocoon after the completion of previous step is cooled to 1～4 DEG C with the speed reducing by 2～4 DEG C per hour, protects at such a temperature
Deposit honeybee cocoon 120 days～360 days；
(4), when, needing, the honeybee cocoon of previous step preservation is taken out, is warming up to 8～12 DEG C with the speed raising 2～4 DEG C per hour,
Keep 4～6 hours；Then under the conditions of the tissue paper with cocoon being transferred to natural lighting or 200lux～500lux the Manual light ring of light
Under border, recover 48～72 hours in the environment of 20 DEG C～30 DEG C of temperature, relative humidity 50%～70%, you can naturally turn into
Scleroderma Xiao et Wu adult.
2. as claimed in claim 1 efficiently preservation Scleroderma Xiao et Wu method it is characterised in that：Insecticide described in step (1) is：
The longicorn beetle (Anoplophora chinensis) of coleoptera Cerambycidae or anoplophora glabripennis (Anoplophora
) or coffee borer (Xylotrechus grayii Whit) glabripennis；Or the Semen Castaneae weevil of Curculionidae
(Curculio davidi Fairmaire) or bamboo curculionid (Otidognathus davidis Fair)；Or Lepidoptera snout moth
The Pyrausta nubilalis (Hubern). (Ostrinia nubilalis) of section；Poplar clearwing moth (the Parathrene tabaniformis of Aegeriidae
[Rottenberg]).
3. as claimed in claim 2 efficiently preservation Scleroderma Xiao et Wu method it is characterised in that：Insecticide described in step (1) is
Semen Castaneae weevil.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610831707.6A CN106376545B (en) | 2016-09-19 | 2016-09-19 | A kind of method of efficient preservation Scleroderma Xiao et Wu |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610831707.6A CN106376545B (en) | 2016-09-19 | 2016-09-19 | A kind of method of efficient preservation Scleroderma Xiao et Wu |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106376545A true CN106376545A (en) | 2017-02-08 |
| CN106376545B CN106376545B (en) | 2019-09-17 |
Family
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610831707.6A Expired - Fee Related CN106376545B (en) | 2016-09-19 | 2016-09-19 | A kind of method of efficient preservation Scleroderma Xiao et Wu |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106962298A (en) * | 2017-05-26 | 2017-07-21 | 何振贤 | A kind of method of the grizzled bar longicorn in utilization Kazakhstan Zhong Tuifengfang Zhiduo County |
| CN107114326A (en) * | 2017-05-17 | 2017-09-01 | 中国林业科学研究院森林生态环境与保护研究所 | A kind of artificial breeding method of the swollen leg honeybee of Monochamus alternatus Hope and the application in preventing and treating forest trunk borer |
| WO2023274430A3 (en) * | 2022-01-18 | 2023-02-23 | 安徽科技学院 | Method for prolonging storage period of egg masses of telenomus remus nixon |
| CN116250510A (en) * | 2023-04-26 | 2023-06-13 | 伊犁哈萨克自治州林业科学研究院 | Artificial rearing method for leg-swelling bees |
| CN116250510B (en) * | 2023-04-26 | 2024-06-04 | 伊犁哈萨克自治州林业科学研究院 | Artificial rearing method for leg-swelling bees |
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| CN103355261A (en) * | 2013-07-30 | 2013-10-23 | 天津拓华生物科技有限责任公司 | Application of thyestillageblerifacldermann larva as host to artificial propagation of scleroderma guani |
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2016
- 2016-09-19 CN CN201610831707.6A patent/CN106376545B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1663368A (en) * | 2004-03-03 | 2005-09-07 | 内蒙古自治区林业科学研究院 | Scleroderma guani parasitism control for loghorn beetle |
| CN103355261A (en) * | 2013-07-30 | 2013-10-23 | 天津拓华生物科技有限责任公司 | Application of thyestillageblerifacldermann larva as host to artificial propagation of scleroderma guani |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107114326A (en) * | 2017-05-17 | 2017-09-01 | 中国林业科学研究院森林生态环境与保护研究所 | A kind of artificial breeding method of the swollen leg honeybee of Monochamus alternatus Hope and the application in preventing and treating forest trunk borer |
| CN107114326B (en) * | 2017-05-17 | 2020-07-10 | 中国林业科学研究院森林生态环境与保护研究所 | Artificial breeding method of Monochamus alternatus Hope and application of method in prevention and treatment of trunk-boring pests in forest trees |
| CN106962298A (en) * | 2017-05-26 | 2017-07-21 | 何振贤 | A kind of method of the grizzled bar longicorn in utilization Kazakhstan Zhong Tuifengfang Zhiduo County |
| WO2023274430A3 (en) * | 2022-01-18 | 2023-02-23 | 安徽科技学院 | Method for prolonging storage period of egg masses of telenomus remus nixon |
| CN116250510A (en) * | 2023-04-26 | 2023-06-13 | 伊犁哈萨克自治州林业科学研究院 | Artificial rearing method for leg-swelling bees |
| CN116250510B (en) * | 2023-04-26 | 2024-06-04 | 伊犁哈萨克自治州林业科学研究院 | Artificial rearing method for leg-swelling bees |
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