CN1256863A

CN1256863A - Extensively aseptic rearing method for domestic fly and its larva

Info

Publication number: CN1256863A
Application number: CN 00100284
Authority: CN
Inventors: 卢永军
Original assignee: Individual
Current assignee: Individual
Priority date: 2000-01-14
Filing date: 2000-01-14
Publication date: 2000-06-21

Abstract

The present invention relates to a large-scale aseptic rearing method of domestic fly and its larva, and is characterized by including the following steps: using the Beijing domestic fly cultured by Inst. of Animals, Chinese Academy of Sciences as fly seed, then utilizing the optimized aseptic fly mumia to be emerged to make eclosion and egg-laying in rearing cage, then collecting and hatching to obtain larva, through a certain cultivating time, controlling body weight of larva, tray dividing, optimizing, screening, aseptic treatment and special culture and propagation so as to implement invented large-scale cultivation of domestic fly.

Description

Housefly and the aseptic cultural method of larva scale thereof

Housefly (Musca domestica vicina Maquart) belongs to that a kind of distributed areas are wide, life cycle is short, fertility is high, adaptability is strong, is easy to the holometabolan of artificial feeding.The used Musca diptera of the present invention, Aristocera, Nuscidae, Musca Beijing housefly.From the latter stage seventies; just the someone propagates the research of housefly and larva thereof artificially; but the technology that is suitable for the batch production breeding scale is not then appeared in the newspapers, and the present invention is practicable from raw material resources, technical feasibility or equipment and economic benefit with regard to its large-scale cultivation.

The purpose of this invention is to provide a kind ofly through Beijing housefly that optimization selects, can be suitable for the aseptic cultural technique of scale, this technology has high yield, low cost, is easy to the characteristics of industrial aquaculture.

Narrate technical scheme of the present invention below in conjunction with embodiment:

The used fly kind of the present invention is drawn the animal institute from the Chinese Academy of Sciences, through the ectogenesis number for after standby.

The raising of adult: will be after preferred sterile fly puparium to be sprouted wings, place the emergence dish, and put it in the adult breeding cage, be furnished with foodstuff dish, drinking-water dish in the breeding cage, pupa can be sprouted wings in common 3～4 days, and the fly pupa of placing in the breeding cage is advisable with about 8000～10000.After treating the pupa emergence about 5%, get final product feeding, feed water, adult foodstuff and water, change once every day.The drinking-water dish is put into sponge or gauze, for the adult drinking-water that stops.Breeding cage will have numbering and indicate lay eggs date and egg laying amount.

The collection of ovum and hatching: 1～3 purpose adult that sprouts wings just begins mating, and take out the emergence dish in the cage this moment, and ovigerous disk is put into cage.Adult lay eggs with every morning 8 up at 3 o'clock in afternoon quantity maximum, be advisable to get the ovum secondary every day, and the ovigerous disk that more renews simultaneously.Adult lays eggs and eliminated at once in back about 15 days.The egg laying amount of adult depends on nutrition condition, the especially larval phase in larva and two stages of adult.It is in 60～70% the medium that the ovum of collecting is seeded in water content equably, under 25～30 ℃ of conditions of room temperature, generally can hatch at 8～12 hours and is larva, and can raise (adjustment cultivation density) this moment according to actual conditions branch dish.Culture plate will have numbering and indicate inoculation time.

The cultivation of larva is cultured: the larva that hatches is 25-30 ℃ cultivating temperature, and moisture content in medium is under the 60-70% condition, cultivates 3-4 days in culture plate, and its whose body weight can increase weight and reach more than 15 milligrams.Cultivate after 2-3 days, excessive as finding the larva cultivation density, then can consider to divide once more dish.Between culture period, the interior temperature of attention medium can not be crossed low or too high, and larval density can not be excessive, otherwise be unfavorable for the normal growth of larva, even cause death.

The seed selection of pupa:

Kind of worm is optimized screening: in the larvae cultivation process, therefrom select individual sturdy, neat independent raising pupate (with weight 40/best below the gram).After giving over to the larva raising pupa that kind of worm uses, cross 16 order dusting covers, the emergence dish of then it being packed into is prepared to sprout wings.

The collection of larva: larvae cultivation is to 3-4 days (also claiming 3 ages or 4 instar larvaes), i.e. timely separated and collected.The characteristics of at first utilizing the negative phototaxis of larva promptly to keep in dark place are placed on culture plate on the operating desk, under the light source irradiation, successively peel off medium, until the exposed a large amount of larvas in culture plate bottom; Screen out residual medium and cross small grub through bolter again, its pack in time is made for uses.

Raise the influence of the temperature, humidity of room temperature, temperature, medium to housefly and larval growth thereof:

Raise room temperature, Temperature Influence:

The cultivation temperature in housefly (adult) vegetative period should be at 20-32 ℃, is advisable with 24-30 ℃, and relative air humidity is advisable with 60-80%, and the border is observed factually, and when cultivating temperature at 24-30 ℃, the food ingestion of adult is the most vigorous, the output maximum.Therefore in breeding process, should control and adjust indoor temperature and humidity, and ventilation in time, pure and fresh with temperature in the holding chamber and air.

The height that the larval growth phase is cultivated temperature and humidity is bigger to the speed influence of its growth.Suitable temperature and humidity, larval growth speed is fast and neat, because the larva happiness is grown in humidity, the temperature adapt circumstance, to absorb enough nutrition.Therefore, should control and cultivate temperature at 20-32 ℃, be good with 25-30 ℃ especially, and humidity should be controlled at about 60-75%.

The temperature of larva medium, the influence of humidity:

The temperature of larva medium, humidity have a significant impact the growth of larva, and generally speaking, in moisture content in medium 50-80% scope, larva all can survive and can grow to pupating.But medium overdrying or the wet excessively growth that all is unfavorable for larva, suitable water content should be between 60-70%, and temperature is between 25-30 ℃.

The lay eggs control of the rhythm and pace of moving things of housefly:

In breeding process, the rhythm and pace of moving things of laying eggs of understanding and grasping housefly is very important, the full egg-laying period rhythm and pace of moving things of housefly, under constant temperature, adult has significantly spawning peak twice, is for the first time after emergence 6-9 days, and egg laying amount accounts for about 29% of total ovum amount of full egg-laying period, for the second time spawning peak is after emergence 12-18 days, and egg laying amount accounts for about 44% of total ovum amount.From the time, adult has spawning peak three times: the peak accounted for about 11% of total ovum amount at 10 o'clock in the morning for the first time; The peak is 2 o'clock in the afternoon for the second time, accounts for about 17% of total ovum amount; The peak accounted for about 11% of total ovum amount at late 10 o'clock for the third time.

, should control and culture temperature, humidity, light application time in the egg-laying period adult.

Points for attention: in culturing overall process, should particularly note, prevent to plant in the breeding cage fly fly escape and the flying into of outside fly.

Claims

1, a kind of housefly and the aseptic cultural method of larva scale thereof; be that " Beijing housefly " cultivated with Chinese Academy of Sciences animal is as the fly kind; sterile fly puparium to be sprouted wings after utilizing it preferred; in breeding cage, make its emergence and lay eggs; collect then and hatching becomes larva, through certain cultivation time, control larva body weight; carry out branch dish, realize large-scale cultivation through optimization, screening, aseptic process, special ectogenesis.

2, a kind of housefly according to claim 1 and the aseptic cultural method of larva scale thereof; it is characterized in that: sterile fly puparium to be sprouted wings after preferred; being to have placed emergence dish breeding cage, is advisable with 8000～10000 in every unit, treats that water is fed in the pupa emergence back feeding about 5%.

3, a kind of housefly according to claim 1 and the aseptic cultural method of larva scale thereof; it is characterized in that: above-mentioned pupa emergence back was put into ovigerous disk on the 1st～3; get ovum every day 2 times; it is in 60～70% the medium that the ovum of collecting is seeded in water content equably; under 25～30 ℃ of conditions, cultivating promptly became larva in 8～12 hours.

4, a kind of housefly according to claim 1 and the aseptic cultural method of larva scale thereof is characterized in that: larva in culture plate, cultivate 3～4 days, when whose body weight reaches more than 15 milligrams, cultivated again 2～3 days.

5, a kind of housefly according to claim 1 and the aseptic cultural method of larva scale thereof is characterized in that: select individual sturdy neat independent raising pupa from the above-mentioned larva of cultivating, cross 16 order dusting cover input emergence dishes.

6, a kind of housefly according to claim 1 and the aseptic cultural method of larva scale thereof is characterized in that: larva becomes the cultivation temperature of housefly at 20～32 ℃, and humidity is advisable with 60～80%.