CN102550495B - Method for culturing and domesticating fly species - Google Patents
Method for culturing and domesticating fly species Download PDFInfo
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- CN102550495B CN102550495B CN2012100548958A CN201210054895A CN102550495B CN 102550495 B CN102550495 B CN 102550495B CN 2012100548958 A CN2012100548958 A CN 2012100548958A CN 201210054895 A CN201210054895 A CN 201210054895A CN 102550495 B CN102550495 B CN 102550495B
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- 238000000034 method Methods 0.000 title claims abstract description 17
- 238000012258 culturing Methods 0.000 title abstract 4
- 230000012447 hatching Effects 0.000 claims abstract description 15
- 244000144972 livestock Species 0.000 claims abstract description 9
- 244000144977 poultry Species 0.000 claims abstract description 9
- 102000002322 Egg Proteins Human genes 0.000 claims description 24
- 108010000912 Egg Proteins Proteins 0.000 claims description 24
- 210000004681 ovum Anatomy 0.000 claims description 24
- 210000003608 fece Anatomy 0.000 claims description 16
- 239000000463 material Substances 0.000 claims description 13
- 241000257159 Musca domestica Species 0.000 claims description 11
- 235000013305 food Nutrition 0.000 claims description 10
- 235000015099 wheat brans Nutrition 0.000 claims description 10
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 9
- 239000010931 gold Substances 0.000 claims description 9
- 229910052737 gold Inorganic materials 0.000 claims description 9
- 239000010871 livestock manure Substances 0.000 claims description 9
- 241000382353 Pupa Species 0.000 claims description 8
- 241001465754 Metazoa Species 0.000 claims description 6
- 238000009395 breeding Methods 0.000 claims description 6
- 230000001488 breeding effect Effects 0.000 claims description 6
- 235000013601 eggs Nutrition 0.000 claims description 6
- 230000035800 maturation Effects 0.000 claims description 6
- 239000002994 raw material Substances 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 241000196324 Embryophyta Species 0.000 claims description 4
- 239000006227 byproduct Substances 0.000 claims description 4
- 230000003203 everyday effect Effects 0.000 claims description 4
- 238000011534 incubation Methods 0.000 claims description 4
- 230000013011 mating Effects 0.000 claims description 4
- 108090000623 proteins and genes Proteins 0.000 claims description 4
- 241000251468 Actinopterygii Species 0.000 claims description 3
- 230000000855 fungicidal effect Effects 0.000 claims description 3
- 239000000417 fungicide Substances 0.000 claims description 3
- 239000002893 slag Substances 0.000 claims description 3
- 239000013589 supplement Substances 0.000 claims description 3
- 241000287828 Gallus gallus Species 0.000 claims description 2
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 2
- 241000209140 Triticum Species 0.000 claims description 2
- 235000021307 Triticum Nutrition 0.000 claims description 2
- 230000000507 anthelmentic effect Effects 0.000 claims description 2
- 239000008280 blood Substances 0.000 claims description 2
- 210000004369 blood Anatomy 0.000 claims description 2
- 235000015277 pork Nutrition 0.000 claims description 2
- 239000002689 soil Substances 0.000 claims description 2
- 241000255925 Diptera Species 0.000 abstract description 7
- 241000894007 species Species 0.000 abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 229940079593 drug Drugs 0.000 abstract 1
- 230000019617 pupation Effects 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
- 230000001502 supplementing effect Effects 0.000 abstract 1
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 4
- 229920002554 vinyl polymer Polymers 0.000 description 4
- 230000001902 propagating effect Effects 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 2
- 238000004064 recycling Methods 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000012286 potassium permanganate Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a method for culturing and domesticating fly species, relating to a method for artificially domesticating wild flies. The method comprises the following steps: collecting wild fly blows; hatching the fly blows; culturing maggots; carrying out drug-resistant screening on the maggots; carrying out pupation and raising; culturing and domesticating flies; and supplementing fly species germplasm. The method has the following advantages: fly species sources can be stably obtained in quantity; high-quality species sources are provided for carrying out resource treatment on livestock and poultry excrement by utilizing maggots on a large scale; and besides, proper fly species are domesticated aiming at actual culture conditions, thus being beneficial to actual production and culture.
Description
Technical field
The present invention relates to a kind of wild flies artificial acclimation method, especially relate to domestication's technology of wild reddish tone gold fly (Chrysomia megcephala) and wild housefly (Musca domestica).
Background technology
At present, livestock and poultry cultivation had become first of China's agricultural pollution source already.The fly maggot efficiency of food conversion is high, and reproduction speed is fast, and protein content is high, and has a liking for coprophagy just, thereby is one of optimal species of feces of livestock and poultry biological treatment.In actual production process, the most important condition of utilizing fly maggot recycling treatment feces of livestock and poultry is to obtain the fly kind provenance that is fit to propagate artificially, the wild flies of namely taming, so the domestication of wild flies is extremely important.Chinese patent CN101779610 discloses a kind of wild housefly domesticating method.Method is: 1, obtains the maggot kind, from lavatory, fishes for fly maggot, be poured on after cleaning in the fly maggot breeding material and cultivate, and ripe rear separation automatically in 2～3 days, the ripe fly maggot of collection is put in plastic basin and sprinkles wheat bran and with woven bag, cover; 2, sterilization, sift out pupa after pupating, with the potassium permanganate liquid disinfectant; 3, hatching fly maggot, be about to pupa and be placed in a small amount of wheat bran of plastic basin covering, treats its nymphosis; 4, domestication fly, cultivated for 4 generations continuously in breeding room, domestication is completed.
In addition; in process of production; except obtaining fly kind provenance, to adapt to certain diet composition, temperature range, enviromental conditions for spawning and limited activity space, fly maggot larva drug resistance etc. also extremely important according to propagating condition domestication fly artificially, is conducive to large-scale cultivation.
Summary of the invention
The cultivation domesticating method that the purpose of this invention is to provide a kind of fly fly kind.
The present invention includes following steps:
1) collect wild fly blow;
2) fly blow hatching;
3) fly maggot is cultivated;
4) fly maggot selection resistance to fungicide;
5) pupate, raise;
6) cultivation of fly domestication;
7) fly kind germplasm supplements;
In step 1) in, the concrete grammar of the wild fly blow of described collection can be: the morning when weather is better, morning when particularly spring and autumn is fine, to collect the ovum material is placed in collection ovum dish, be positioned over the place that open-air fly often haunts, after fly lays eggs, collect the collection ovum dish that contains fly blow in the time of at dusk; Describedly be positioned over the place that open-air fly often haunts and preferably be positioned over and sheltered from heat or light, avoid the place of direct sunlight.
In step 2) in, the concrete grammar of described fly blow hatching can be: the fly blow that will collect the collection of ovum dish is placed in incubation plate, take fresh pig manure as hatching medium, and with moistening wheat bran, cover on fly blow, then putting into incubator box hatches, fly blow has been hatched into larva substantially, take out incubation plate or will hatch between humidity turn down, avoid the fly maggot larva to escape greatly because of humidity; The temperature of described hatching can be 30～36 ℃, and the humidity of hatching can be 75%～90%, and the time of hatching can be 10～15h.
In step 3) in, the concrete grammar that described fly maggot is cultivated can be: the fly maggot larva after hatch is put into the cultivation of cultivation material, treats that it reaches maturity; Described cultivation material can be selected at least a in fresh feces of livestock and poultry, agricultural byproducts, animal raw materials etc., described feces of livestock and poultry can be selected from a kind of in pig manure, chicken manure etc., described agricultural byproducts can be selected from least a in wheat skin, vinasse, bean dregs etc., optional a kind of in animal blood, pork, the flesh of fish etc. of described animal raw materials; It is for housefly that described fully-developed requires, and the fly maggot body surface is yellow by leucismus, more than Individual Quality reaches 20mg, namely is considered as maturation; , for reddish tone gold fly, more than the fly maggot Individual Quality reaches 85mg, namely be considered as maturation.
In step 4) in, the concrete grammar of described fly maggot selection resistance to fungicide can be: when the fly maggot provenance is cultivated domestication, select plant to use the ight soil raw material during anthelmintic to cultivate, the also smooth growth fully-developed fly maggot of surviving is the provenance that filters out.
In step 5) in, the described concrete grammar of pupating, raising can be: collect ripe fly maggot, pupate after sterilization, can put into the fly breeding room and raise after having pupated; The temperature of pupating can be 20～35 ℃, and the temperature of raising can be 18～38 ℃, adds auxiliary material in the process of pupating, and described auxiliary material can adopt at least a in fly maggot slag, moistening wheat bran etc.
In step 6) in, the concrete grammar of the cultivation domestication of described fly can be: pupa is put into kind of a room, as 1st generation fly kind, when fly starts to sprout wings, can put into food dish, water pond etc., take food for fly, and set time every day adds and replacing, plants room and sweeps 1 time in every 2～3 days; After emergence, after housefly 4～7 days, reddish tone gold fly started to lay eggs in a large number after 10 days, at this moment put into collection ovum dish collection ovum, and the ovum of having collected is pressed step 2)～6) operation, obtain 2nd generation fly kind, by this analogize obtain the 3rd generation the fly kind, the 4th generation the fly kind, the 5th generation fly kind etc., in fly kind domestication process, per generation the fly kind should separately cultivate, avoid raising together with, can accelerate domestication speed.
In step 7) in, the concrete grammar that described fly kind germplasm supplements can be: by step 1) collect open-air fly ovum, after cultivating into ripe fly maggot and nymphosis, fill in kind of room together with the pupa of the good fly kind of original domestication, make its mating, increase fly kind gene diversity, avoid the fly kind to degenerate.
The invention has the advantages that and can stablize, obtain in a large number fly kind provenance, for scale utilizes fly maggot recycling treatment feces of livestock and poultry, provide the high-quality provenance; In addition, for actual cultivating condition, tame out suitable fly kind, be conducive to the actual production cultivation.
Embodiment
Embodiment 1
The domestication of fly kind is carried out in mound pipunculid maggot production base, Nanjing County, Fujian Province, in the morning in November fresh pig manure being placed on vinyl disc, dividing and makes 10 dishes, and under near the tree shade the placement pig farm, the collection ovum dish collection that will be mainly the housefly fly blow when the dusk is fetched.And with a small amount of moistening wheat bran, cover on fly blow, then put into incubator box, keep 30～36 ℃ of temperature, humidity 75%～90%.In the 2nd day morning, fly blow is hatched substantially as the fly maggot larva, the fly maggot larva after hatching is put into the proper amount of fresh pig manure cultivate, and approximately after 4 days, housefly reaches maturity.Collect ripe fly maggot, putting into vinyl disc after sterilization and adding a small amount of fly maggot slag is that auxiliary material is pupated, and can put into the fly breeding room and raise after having pupated.When starting have fly to sprout wings out, can put into the confession flies such as food dish, water pond and take food, food dish and water pond added between 4:30～5:30 point and change afternoon every day, planted room and swept 1 time in every 2～3 days.Approximately started to lay eggs in 4th after sprouting wings, in the morning approximately 8:30 put into collection ovum dish collection ovum.The ovum collected, by the above-mentioned steps operation, is obtained 2nd generation fly kind, by this analogize obtain the 3rd generation the fly kind, the 4th generation the fly kind, the 5th generation fly kind etc.Domestication fly has out comparatively adapted to propagating environment artificially after about the 4th generation, and this moment, domestication was completed substantially.In raising afterwards, collect per month appropriate open-air housefly fly blow, wait cultivate maturation and pupate after put into the cultivation of sprouting wings of kind of room together with the housefly pupa of former artificial raising, make its mating, increase fly kind gene diversity, avoid the fly kind to degenerate.
Embodiment 2
The domestication of fly kind is carried out in fly kind cultivation base, quiet city in the Nanjing County, Fujian Province, moistening wheat bran is sprinkled during a small amount of milk powder is placed on vinyl disc in the morning in October, divide and make 10 dishes, be positioned under the shade in the place that open-air reddish tone gold fly often haunts, the collection ovum dish that will contain reddish tone gold fly fly blow when the dusk is collected and is fetched.And with a small amount of moistening wheat bran, cover on fly blow, then put into incubator box, keep 30～36 ℃ of temperature, humidity 75%～90%.In the 2nd day morning, fly blow is hatched substantially as the fly maggot larva, the fly maggot larva after hatching is put into the proper amount of fresh pig manure cultivate, and approximately after 5～6 days, fly maggot is reached maturity.Collect ripe fly maggot, putting into vinyl disc after sterilization and adding a small amount of moistening wheat bran is that auxiliary material is pupated, and can put into the fly breeding room and raise after having pupated.When starting have fly to sprout wings out, can put into the confession flies such as food dish, water pond and take food, dish adds and replacing between 4:30～5:30 point afternoon every day with water pond, and the kind room was swept 1 time in every 2～3 days.Approximately started to lay eggs in 7～10 days after sprouting wings, in the morning approximately 8:30 put into collection ovum dish collection ovum.The ovum collected, by the above-mentioned steps operation, is obtained 2nd generation fly kind, by this analogize obtain the 3rd generation the fly kind, the 4th generation the fly kind, the 5th generation fly kind etc.Domestication fly has out comparatively adapted to propagating environment artificially after about 4～5 generations, and this moment, domestication was completed substantially.In raising afterwards, collect per month the fly blow of appropriate open-air reddish tone gold fly, wait cultivate maturation and pupate after put into the cultivation of sprouting wings of kind of room together with the pupa of the reddish tone gold fly of former artificial raising, make its mating, increase fly kind gene diversity, avoid the fly kind to degenerate.
Claims (3)
1. the cultivation domesticating method of fly fly kind is characterized in that comprising the following steps:
1) collect wild fly blow: in the morning when fine, will collect the ovum material and be placed in collection ovum dish, be positioned over and sheltered from heat or light, avoid the place of direct sunlight, after fly lays eggs, collect the collection ovum dish that contains fly blow in the time of at dusk;
2) fly blow hatching: the fly blow that will collect the collection of ovum dish is placed in incubation plate, take fresh pig manure as hatching medium, and with moistening wheat bran, cover on fly blow, then putting into incubator box hatches, fly blow has been hatched into larva substantially, take out incubation plate or will hatch between humidity turn down, avoid the fly maggot larva to escape greatly because of humidity;
3) fly maggot is cultivated: the fly maggot larva after hatch is put into the cultivation of cultivation material, treats that it reaches maturity; Described cultivation material is selected at least a in fresh feces of livestock and poultry, agricultural byproducts, animal raw materials; Described feces of livestock and poultry is selected from a kind of in pig manure, chicken manure, and described agricultural byproducts are selected from least a in wheat skin, vinasse, bean dregs, and described animal raw materials is selected from a kind of in animal blood, pork, the flesh of fish;
4) fly maggot selection resistance to fungicide: when the fly maggot provenance is cultivated domestication, select plant to use the ight soil raw material during anthelmintic to cultivate, the also smooth growth fully-developed fly maggot of surviving is the provenance that filters out;
5) pupate, raise: collect ripe fly maggot, pupate after sterilization, namely put into the fly breeding room and raise after having pupated; The temperature of pupating is 20～35 ℃, and the temperature of raising is 18～38 ℃, adds auxiliary material in the process of pupating, and described auxiliary material adopts at least a in fly maggot slag, moistening wheat bran;
6) cultivation of fly domestication: pupa is put into kind of a room,, as 1st generation fly kind, when fly starts to sprout wings, can put into food dish, water pond, for fly, take food, and set time every day adds and replacing, plants room and sweeps 1 time in every 2～3 days; After emergence, housefly is after 4～7 days, and reddish tone gold fly started to lay eggs in a large number after 10 days, at this moment puts into collection ovum dish collection ovum, and the ovum of having collected is pressed step 2)～6) operate, obtain 2nd generation fly kind, by this analogize obtain the 3rd generation the fly kind, the 4th generation the fly kind, the 5th generation the fly kind;
7) fly kind germplasm supplements: collect open-air fly ovum by step 1), after cultivating into ripe fly maggot and nymphosis, fill in kind of room together with the pupa of the good fly kind of original domestication, make its mating, increase fly kind gene diversity, avoid the fly kind to degenerate.
2. the cultivation domesticating method of fly fly kind as claimed in claim 1, is characterized in that in step 2) in, the temperature of described hatching is 30～36 ℃, and the humidity of hatching is 75%～90%, and the time of hatching is 10～15h.
3. the cultivation domesticating method of fly fly kind as claimed in claim 1, is characterized in that described fully-developed requirement is, for housefly, the fly maggot body surface is yellow by leucismus, more than Individual Quality reaches 20mg, namely is considered as maturation; , for reddish tone gold fly, more than the fly maggot Individual Quality reaches 85mg, namely be considered as maturation.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012100548958A CN102550495B (en) | 2012-03-05 | 2012-03-05 | Method for culturing and domesticating fly species |
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| CN2012100548958A CN102550495B (en) | 2012-03-05 | 2012-03-05 | Method for culturing and domesticating fly species |
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| CN102550495A CN102550495A (en) | 2012-07-11 |
| CN102550495B true CN102550495B (en) | 2013-11-20 |
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| CN103125450B (en) * | 2013-02-07 | 2014-11-26 | 浙江大学 | Fly maggot cultivating method using bean dregs |
| CN103141446B (en) * | 2013-03-20 | 2015-01-14 | 周辛平 | Method and device for producing fly larvae |
| CN103181370B (en) * | 2013-04-07 | 2015-02-11 | 哈尔滨艾利姆农业科技有限公司 | Culture medium for housefly larve |
| CN103181371B (en) * | 2013-04-07 | 2015-03-04 | 哈尔滨艾利姆农业科技有限公司 | Culture medium for housefly egg-collecting bed |
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| CN104285913A (en) * | 2014-11-04 | 2015-01-21 | 凌征雄 | Housefly breeding method |
| CN106035249A (en) * | 2016-08-14 | 2016-10-26 | 何丽凌 | Method for breeding housefly and maggot biological feed for chickens |
| CN106035248A (en) * | 2016-08-14 | 2016-10-26 | 何丽凌 | Method for breeding housefly maggots serving as biological feed for horses |
| CN106070087A (en) * | 2016-08-14 | 2016-11-09 | 何丽凌 | A kind of duck biological feedstuff housefly maggot cultural method |
| CN113841607B (en) * | 2021-09-08 | 2023-03-24 | 上海惠和种业有限公司 | Method for seed production and pollination of vegetables by using flies |
| CN114868708A (en) * | 2022-04-15 | 2022-08-09 | 中南大学 | Breeding and domesticating method for Boettcherisca peregrina |
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